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IRON BIOAVAILABILITY FROM POTATO: A COMPLEX INTERPLAY BETWEEN ENHANCERS AND INHIBITORS FROM THE MATRIX Lisa Miranda, Christian Iammarino, Hannah Deußer, Johanna Ziebel, Danièle Evers Centre de Recherche Public-Gabriel Lippmann, Départment EVA, 41, rue du Brill, L-4422 Luxembourg; miranda@lippmann.lu Keywords: ascorbic acid, Caco-2 cells, chlorogenic acid, Iron, Potato, Dietary iron deficiency is the most common nutritional disorder in the world. Increasing the amount of bioavailable dietary iron could prevent iron deficiency and anaemia. Potato is one of the most consumed crops worldwide; it has been described to be a good source of minerals, such as potassium and magnesium and some cultivars have been shown to be rich in iron and zinc. The aim of this work is to evaluate iron bioavailability for human consumption from different potato cultivars and to study the impact of enhancers of iron bioavailability (e.g. ascorbic acid) or inhibitors present in potato, such as chlorogenic acid. Caco-2 cells were used to evaluate the bioaccessibility of iron; the abundance of the ferritin protein was used as a marker of iron uptake. Investigations were done on in vitro digested potato extracts as well as on separated compounds exposed to Caco-2 cells. ICP-MS, Elisa and western blot results on Caco-2 cells show that Fe(II) is absorbed in presence or not of ascorbic acid; however, its absorption is enhanced by ascorbic acid. Fe(III) uptake is mostly due to the presence of ascorbic acid. Chlorogenic acid inhibits Fe(III) and Fe(II) uptake induced by ascorbic acid. Different potato cultivars submitted to an in vitro gastrointestinal digestion displayed different polyphenol profiles; results will be presented on the iron uptake by Caco-2 cells as influenced by these different profiles and thus illustrate the impact and nature of enhancers and inhibitors of iron uptake in potato.
HOW TO ENHANCE NUTRIENT ASSIMILATION IMPROVING BIOAVAILABILITY Juan Navarro-Aviñó 1,2 , C. Cioranu 2 , Felix Macías 2 , Pilar Hernandez 2 , Luis Sanz 2 , Amparo García 2 , Leonor Lapeña 1 , Pilar García-Agustín 1 . 1 Department of Experimental Sciences, Area of Vegetable Physiology, ESTCE, University Jaume I Post Code 12071, Castellón, Spain 2 ABBA Gaia, UPV, Camino de Vera s.n., Post Code 46022 Valencia, Spain Corresponding author. Tel.: +34 96 1109969; E-mail address: jpavinyo@upvnet.upv.es (J. Navarro-Aviñó) or avino@camn.uji.es. Keywords: bioavailability, Cupper, diet, micronutrients, Zinc. Abstract This work shows that, increase of availability of micronutrients, such as Cu and Zn, displays direct correlation between the accumulation capacity of a species (insertion of a gene increases the internal concentration of nutrients), and increased bioavailability. Therefore bioavailability can be increased by inserting this single gene. Objectives The ultimate goal is to find a method, transportable to food crops (human diet), to increase biovailability Methods Wild type, and plants overexpressing a phytochelatin synthase, were grown in pots containing two types of industrial sludge. After several months of growth, the accumulation of micronutrients inside the plants, growth medium and in leachate were measured. Results and conclusions There is a greater presence of micronutrients in the leachate from pots containing overexpressed-gene insertion plants and a greater decrease in the concentration in the growth medium. Since the amount of micronutrient accumulated is greater in the plants which contain the gene insert, can be concluded that gene overexpression, results in higher bioavailability. The percentage reduction of micronutrient in the middle of the pot, ranged from 78 to 87%. Consequently, this gene insertion may be a way to increase the efficiency of accumulation of trace elements in a plant, reducing costs and increasing the nutritional value of a crop.
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PROCEEDING OF
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INTERNATIONAL SCIENTIFIC COMMITTEE:
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Second Annual Conference and MC Mee
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16:30 16:30- Zeshan Hassan, Sangita
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processes 16:10 Coffee break 16:30
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HOW TO INFLUENCE THE CD CONTENT IN
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ZN UPTAKE BY WHEAT IN THE PRESENCE
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NANOSIMS: A TECHNIQUE FOR SUBCELLUL
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IN VITRO MINERAL AVAILABILITY IN PE
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RECENT ADVANCES IN COMPARTMENTATION
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production, breeding and processing
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SUBSTANTIAL NATURAL VARIATION IN MI
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ADAPTIVE MECHANISMS OF HYPERACCUMUL
Page 29 and 30: BREEDING CROPS FOR BETTER NUTRITION
Page 31 and 32: Fig 1, 190x showing biochar’s var
Page 33 and 34: V. SIMULTANEOUS BIOFORTIFICATION OF
Page 35 and 36: PCP1 AND ATOSA1: PROTEINS INVOLVED
Page 37 and 38: FROM ENZYMATIC BROWNING IN FRUITS A
Page 39 and 40: PROGRESS IN THE UNDERSTANDING OF CA
Page 41 and 42: THE ROLE OF BIOCHAR IN THE PHYTOREM
Page 43 and 44: PHYSIOLOGICAL CHARACTERIZATION AND
Page 45: ARSENIC IN RICE: GASTROINTESTINAL B
Page 49 and 50: SALINITY AND MYCORRHIZAL SYMBIOSIS:
Page 51 and 52: ALIMURGIC HERBS AS POTENTIAL SOURCE
Page 53 and 54: EFFECTS OF BIOCHAR AND GREENWASTE C
Page 55 and 56: UPTAKE OF ARSENIC INTO TOMATO PLANT
Page 57 and 58: EFFECTS OF SELENIUM ON THE GROWTH P
Page 59 and 60: EFFECT OF ADDITION OF SULPHUR FERTI
Page 61 and 62: I. SIMULTANEOUS BIOFORTIFICATION OF
Page 63 and 64: EFFECTS OF BIO-(PHYTO-, FUNGI) GENI
Page 65 and 66: ASSESSMENT OF HEAVY METAL (PB, CD,
Page 67 and 68: ZINC FLUXES INTO THE DEVELOPING BAR
Page 69 and 70: THE PRESENCE OF COPPER (Cu 2+ ) IN
Page 71 and 72: DETECTION OF CADMIUM DISTRIBUTION I
Page 73 and 74: DETERMINATION OF POLLUTION OF DRINK
Page 75 and 76: EARLY RESPONSES IN ROOT MERISTEM OF
Page 77 and 78: BUCKWHEAT PRODUCTION ON ACID AND LO
Page 79: THE IMPACT OF HEAVY METALS ON RED C
Page 83 and 84: CONTROLLING CADMIUM ACCUMULATION IN
Page 85 and 86: RESPONSES OF SORGHUM PLANTS TO STRE
Page 87 and 88: TO BE ECO, OR NOT TO BE? - SOME ASP
Page 89 and 90: III. SIMULTANEOUS BIOFORTIFICATION
Page 91 and 92: TRACE ELEMENTS, GLUTATHIONE AND DIA
Page 93 and 94: COMPOST INCORPORATION IN SOIL DECRE
Page 95 and 96: INFLUENCE OF PUTRESCINE APPLICATION
Page 97 and 98: POSSIBLE NICOTIANAMINE INVOLVEMENT
Page 99 and 100: NUTRITIONAL QUALITY OF SELECTED SPR
Page 101: HOW DOES THE GREEN REVOLUTION AFFEC
Page 105 and 106: Aarts Mark; Lab. of Genetics, Wagen
Page 107 and 108: Lacatusu Radu; National Research In
Page 109: Schwitzguebel Jean-Paul; EPFL, Labo
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