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INVESTIGATIONS OF FERRITIN GENE EXPRESSION IN TWO SOLANUM TUBEROSUM CULTIVARS DIFFERING IN TUBER IRON CONTENT Sylvain Legay, Danièle Evers Centre de Recherche Public-Gabriel Lippmann Department ‘Environment and Agro-biotechnologies’ 41, rue du Brill L-4422 Belvaux, Luxembourg Keywords: ferritin, gene expression, iron, potato Iron deficiency is the most important micronutrient deficiency in the world especially in developing countries where more than 50% of the anemias are thought to be due to iron deficiency. Potato is an important staple food and constitutes a good source of potassium and vitamin C, but remains less valuable as a source of iron. Thus, iron biofortification of potato tubers is a crucial strategy for these populations. Ferritin proteins are known to be one of the main components of the iron transport and storage in plant. In potato, until now, the involvement of ferritins in tuber iron accumulation has not been well described. The present work aimed to study ferritin gene expression in tubers with low and high iron content. Two Solanum tuberosum cultivars (Princess and Amandine) were evaluated for the iron content in tubers. In the peel, a higher iron content was observed in Princess (38.97µg/g DW) compared to Amandine (29.82µg/g DW). In the flesh, this trend remained the same with higher amounts (26.57µg/g DW) in Princess compared to 22.33µg/g DW in Amandine. Using an in silico approach, three different ferritin isoforms were isolated from the EST databases and from each isoform, specific qPCR primers were designed. All the ferritin isoforms were expressed in the tubers and seemed to be correlated with iron content.
EARLY RESPONSES IN ROOT MERISTEM OF PISUM SATIVUM AND ARABIDOPSIS THALIANA INDUCED BY COPPER AND SELENIUM Nóra Lehotai, Andrea Pető, Gábor Feigl, Máté Weisz, László Erdei, Zsuzsanna Kolbert Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, P.O.B. 654., 6701 Szeged, Hungary E-mail: lehotai.nora@gmail.com Keywords: long-term copper exposure, long-term selenium exposure, morphological parameters, nitric oxide, viability Selenium (Se) is an essential trace element for animals and humans, which enters the food chain through plants. High excess of this metalloid can be toxic, however Se plays an important role in preventing against a number of diseases. Copper (Cu) is an essential microelement in plants, but at a toxic level its symptoms are dangerous for human health as well. Its deficiency is rare but a high concentration of it may cause nausea and diarrhea, tissue injury and disease, liver damage etc. In our experimental system we used two pea cultivars (Pisum sativum L. cv. Rajnai törpe = Petit Provencal and. cv. Lincoln) and wild type (Col-0) Arabidopsis thaliana as model organism to investigate the physiological responses to long-term copper and short- and long-term selenium exposure. After treatment with different concentrations of Cu and Se, we studied growth parameters, biomass, the level of nitric oxide (NO)-, reactive oxygen species (ROS) and viability in the meristem cells of the plants using in vivo and in situ microscopic methods. We concluded that the effect of treatments is time- and concentration dependent in this stress condition. The increasing concentration of Se/Cu decreased the viability and this lead to serious growth inhibition and decreased biomass.
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PROCEEDING OF
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INTERNATIONAL SCIENTIFIC COMMITTEE:
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Second Annual Conference and MC Mee
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16:30 16:30- Zeshan Hassan, Sangita
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processes 16:10 Coffee break 16:30
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HOW TO INFLUENCE THE CD CONTENT IN
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ZN UPTAKE BY WHEAT IN THE PRESENCE
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NANOSIMS: A TECHNIQUE FOR SUBCELLUL
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IN VITRO MINERAL AVAILABILITY IN PE
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RECENT ADVANCES IN COMPARTMENTATION
Page 23 and 24: production, breeding and processing
Page 25 and 26: SUBSTANTIAL NATURAL VARIATION IN MI
Page 27 and 28: ADAPTIVE MECHANISMS OF HYPERACCUMUL
Page 29 and 30: BREEDING CROPS FOR BETTER NUTRITION
Page 31 and 32: Fig 1, 190x showing biochar’s var
Page 33 and 34: V. SIMULTANEOUS BIOFORTIFICATION OF
Page 35 and 36: PCP1 AND ATOSA1: PROTEINS INVOLVED
Page 37 and 38: FROM ENZYMATIC BROWNING IN FRUITS A
Page 39 and 40: PROGRESS IN THE UNDERSTANDING OF CA
Page 41 and 42: THE ROLE OF BIOCHAR IN THE PHYTOREM
Page 43 and 44: PHYSIOLOGICAL CHARACTERIZATION AND
Page 45: ARSENIC IN RICE: GASTROINTESTINAL B
Page 49 and 50: SALINITY AND MYCORRHIZAL SYMBIOSIS:
Page 51 and 52: ALIMURGIC HERBS AS POTENTIAL SOURCE
Page 53 and 54: EFFECTS OF BIOCHAR AND GREENWASTE C
Page 55 and 56: UPTAKE OF ARSENIC INTO TOMATO PLANT
Page 57 and 58: EFFECTS OF SELENIUM ON THE GROWTH P
Page 59 and 60: EFFECT OF ADDITION OF SULPHUR FERTI
Page 61 and 62: I. SIMULTANEOUS BIOFORTIFICATION OF
Page 63 and 64: EFFECTS OF BIO-(PHYTO-, FUNGI) GENI
Page 65 and 66: ASSESSMENT OF HEAVY METAL (PB, CD,
Page 67 and 68: ZINC FLUXES INTO THE DEVELOPING BAR
Page 69 and 70: THE PRESENCE OF COPPER (Cu 2+ ) IN
Page 71 and 72: DETECTION OF CADMIUM DISTRIBUTION I
Page 73: DETERMINATION OF POLLUTION OF DRINK
Page 77 and 78: BUCKWHEAT PRODUCTION ON ACID AND LO
Page 79 and 80: THE IMPACT OF HEAVY METALS ON RED C
Page 81 and 82: HOW TO ENHANCE NUTRIENT ASSIMILATIO
Page 83 and 84: CONTROLLING CADMIUM ACCUMULATION IN
Page 85 and 86: RESPONSES OF SORGHUM PLANTS TO STRE
Page 87 and 88: TO BE ECO, OR NOT TO BE? - SOME ASP
Page 89 and 90: III. SIMULTANEOUS BIOFORTIFICATION
Page 91 and 92: TRACE ELEMENTS, GLUTATHIONE AND DIA
Page 93 and 94: COMPOST INCORPORATION IN SOIL DECRE
Page 95 and 96: INFLUENCE OF PUTRESCINE APPLICATION
Page 97 and 98: POSSIBLE NICOTIANAMINE INVOLVEMENT
Page 99 and 100: NUTRITIONAL QUALITY OF SELECTED SPR
Page 101: HOW DOES THE GREEN REVOLUTION AFFEC
Page 105 and 106: Aarts Mark; Lab. of Genetics, Wagen
Page 107 and 108: Lacatusu Radu; National Research In
Page 109: Schwitzguebel Jean-Paul; EPFL, Labo
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