Book with abstracts from the COST Action 0905 meeting in ... - UMB

I. SIMULTANEOUS BIOFORTIFICATION OF Zn AND Fe IN
Triticum aestivum L. – A TRANSGENERATION SURVEY
Ana Luísa Fernando 1 , Fernanda Simões 2 , Ana Rita Costa 2 , José Matos 2 , Fernando
Henrique Reboredo 1 , António Eduardo Leitão 3 , José Cochicho Ramalho 3 , Ana Sofia
Almeida 2 , Benvindo Maçãs 2 , José Prates Coutinho 2 , Paula Scotti-Campos 2 , Isabel P. Pais 2 ,
Maria Manuela Abreu da Silva 4 , Maria Paula Duarte 1 , Luis Filipe Goulão 3 , Ana Isabel
Ribeiro 2 , Fernando Cebola Lidon 1
1 DCTB, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da
Torre, 2829-516 Caparica, Portugal.
2 Instituto Nacional de Recursos Biológicos, Estrada de Gil Vaz, Apartado 6, 7350-591
Elvas / Estrada do Paço do Lumiar, 22 Ed. 5, 1649-038 Lisboa / URGEMP, Quinta do
Marquês, 2784-505, Oeiras, Portugal.
3 Centro de Ecofisiologia, Bioquímica e Biotecnologia Vegetal, Instituto de Investigação
Científica Tropical, Quinta do Marquês, 2784-505 Oeiras, Portugal.
4 ESE Almeida Garrett, Grupo Universidade Lusófona, COFAC, Palácio de Santa Helena,
Largo do Sequeira nº 7, Lisboa, Portugal.
Keywords: Iron, Triticum biofortificatiom, Scanning electron microscopy, Zinc
Certified seeds of Triticum aestivum L. cv Nabão (0Ts) were grown in walk-in growth
chamber (EHHF10000, Aralab, Portugal), under environmental controlled conditions (80%
RH; 24/20ºC day/night temperatures; PPFD of ca 700 µmol m -2 s -1 , 12 h photoperiod), in
pots irrigated with a standard solution (1Ts), during 1 month following germination.
Thereafter, until the physiological maturity, half of the pots kept the same irrigation
solution (1Ts), while the other half was submitted to a five fold concentration of all the
nutrients (1T5s). The seeds 1T5s were sown and grown again with the initial standard
nutrient solution (2Ts) and with solutions containing 5, 7, 10 and 13 times fold the
concentration of all the nutrients (2T5s, 2T7s, 2T10s and 2T13s, respectively). It was
found that, relatively to 0Ts, in the grains of 1Ts, 1T5s, 2Ts, 2T5s, 2T7s, 2T10s and 2T13s,
Zn concentration increased about 7.18, 6.10, 9.92, 8.87, 4.73, 9.00 and 9.23 fold, whereas
Fe augmented 1.01, 1.35, 3.91, 4.95, 5.10, 8.98 and 11.06 times, respectively.
Additionally, by SEM it was established that seeds of the 2T7s, 2T10 and 2T13s became
increasingly shrunken. Although, a higher development of the spikelets occurred in the
ears of 2T5s and 2T7s, a genetic homogeneity was found to persist at DNA level, as judged
by AFLP markers. Nevertheless, differences at the transcription level are expected and will
be evaluated in a near future. Considering the wheat commercial relevance for the farmers,
it is concluded that, under the defined growth conditions, 2T5s is the treatment that might
fit best a simultaneous biofortification of Zn and Fe.