Book with abstracts from the COST Action 0905 meeting in ... - UMB
Book with abstracts from the COST Action 0905 meeting in ... - UMB
Book with abstracts from the COST Action 0905 meeting in ... - UMB
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TRANSPORT AND DEPOSITION OF IRON AND ZINC IN THE WHEAT<br />
AND BARLEY GRAIN<br />
IDENTIFICATION OF THE TRANSCRIPTOME CORRELATED WITH HEAVY<br />
METAL TRANSPORT GENE MANIPULATION FOR IMPROVED NUTRITIONAL<br />
VALUE OF THE WHEAT AND BARLEY GRAIN<br />
Søren Borg, Behrooz Darbani, Birgitte Tauris, Shah<strong>in</strong> Noeparvar, Preben B. Holm<br />
The University of Aarhus, Faculty of Science and Technology, Department of Molecular<br />
Biology and Genetics, Research Centre Flakkebjerg, DK-4200 Slagelse, Denmark<br />
The research area comprises molecular characterization of genes encod<strong>in</strong>g z<strong>in</strong>c and<br />
iron transporters and genes <strong>in</strong>volved <strong>in</strong> z<strong>in</strong>c and iron b<strong>in</strong>d<strong>in</strong>g and deposition <strong>in</strong> <strong>the</strong> develop<strong>in</strong>g<br />
barley gra<strong>in</strong> and <strong>the</strong> manipulation of <strong>the</strong>se genes by transformation to improve <strong>the</strong> z<strong>in</strong>c and<br />
iron content of <strong>the</strong> barley endosperm. We have previously performed microarray studies based<br />
on laser capture microdissection and have established <strong>the</strong> first roadmap for z<strong>in</strong>c and iron<br />
transport <strong>in</strong>to <strong>the</strong> barley gra<strong>in</strong> (Tauris et al.2009; Borg et al.2009). We have as one of our<br />
primary goals to develop this roadmap fur<strong>the</strong>r by <strong>in</strong>troduc<strong>in</strong>g next generation sequenc<strong>in</strong>g on<br />
laser capture microdissected tissues compris<strong>in</strong>g <strong>the</strong> transport pathway <strong>from</strong> <strong>the</strong> vegetative<br />
tissues <strong>in</strong>to <strong>the</strong> endosperm. Our second objective is to use this knowledge to improve iron and<br />
z<strong>in</strong>c content and bioavailability of <strong>the</strong> endosperm by gene manipulation by transformation.<br />
Our hypo<strong>the</strong>sis is that it is possible to <strong>in</strong>crease <strong>the</strong> content of essential m<strong>in</strong>erals <strong>in</strong> <strong>the</strong> wheat<br />
endosperm by transgenic modulation of m<strong>in</strong>eral transporters and deposition <strong>in</strong> <strong>the</strong> endosperm.<br />
Gene manipulation:<br />
• Bypass <strong>the</strong> normal deposition of z<strong>in</strong>c <strong>in</strong> <strong>the</strong> prote<strong>in</strong> storage vacuole of <strong>the</strong> aleurone by<br />
tissue-specific down-regulation of <strong>the</strong> vacuolar z<strong>in</strong>c transporter, MTP1.<br />
• Generate barley plants over express<strong>in</strong>g <strong>the</strong> HvHMA2 z<strong>in</strong>c transporter us<strong>in</strong>g unload<strong>in</strong>g<br />
zone specific promoters.<br />
• Generation of transgenic wheat overexpress<strong>in</strong>g <strong>the</strong> wheat TaFer1 gene under <strong>the</strong><br />
control of <strong>the</strong> wheat high molecular weight gluten<strong>in</strong>e 1Dx5 promoter <strong>in</strong> modern<br />
CIMMYT wheat l<strong>in</strong>es.<br />
• Bypass <strong>the</strong> normal deposition of iron <strong>in</strong> <strong>the</strong> prote<strong>in</strong> storage vacuole of <strong>the</strong> aleurone by<br />
tissue-specific down-regulation of vacuolar iron transporter, Vit1.<br />
• Cross <strong>the</strong> transgenic l<strong>in</strong>es <strong>with</strong> l<strong>in</strong>es express<strong>in</strong>g phytase to improve <strong>the</strong> bioavailability<br />
of divalent m<strong>in</strong>eral cations <strong>in</strong> <strong>the</strong> digestive tract of humans and livestock<br />
The work is supported by HarvestPlus, 2033 K Street, NW, Wash<strong>in</strong>gton DC 20006-1002,<br />
USA<br />
References<br />
Borg S, Br<strong>in</strong>ch-Pedersen H, Tauris B and Holm P B 2009 Iron transport, deposition and<br />
bioavailability <strong>in</strong> <strong>the</strong> wheat and barley gra<strong>in</strong>. Plant and Soil 325, 15-24.<br />
Tauris B, Borg S, Gregersen P L and Holm P B 2009 A roadmap for z<strong>in</strong>c traffick<strong>in</strong>g <strong>in</strong> <strong>the</strong><br />
develop<strong>in</strong>g barley gra<strong>in</strong> based on laser capture microdissection and gene expression profil<strong>in</strong>g.<br />
Journal of Experimental Botany 60, 1333-1347.