Book with abstracts from the COST Action 0905 meeting in ... - UMB

More documents

Recommendations

Info

CHARACTERIZATION OF ARABIDOPSIS MUTANTS AFFECTED IN SEED IRON STORAGE. Viviane Mary 1 , Magali Schnell Ramos 1 , Jérôme Giraudat 1 , Hicham Khodja 2 , Sébastien Thomine 1 1 Institut des Sciences du Végétal, CNRS, Gif-sur-Yvette, France, 2 CEA, IRAMIS, SIS2M, LEEL and CNRS, UMR 3299, SIS2M, LEEL, F-91191 Gif-sur- Yvette, France. Keywords: Iron, Mutants, Seed, Transport, Vacuole. To improve seed iron (Fe) content and availability, it is crucial to decipher the mechanisms which control Fe storage during seed development. In Arabidopsis thaliana seeds, Fe is stored in the vacuoles of cells surrounding the vasculature of the embryo. The AtVIT1 transporter is involved in Fe influx into vacuoles (Kim et al., Science 314:1295-98), while AtNRAMP3 and AtNRAMP4 function redundantly in Fe retrieval from vacuoles during germination (Lanquar et al., EMBO J. 24:4041-51). When germinated under Fe deficient conditions, nramp3nramp4 double mutant development is arrested as a consequence of impaired Fe mobilization. To identify novel genes involved in seed Fe homeostasis, we screened an EMS mutagenized population of nramp3nramp4 for mutations restoring the growth of nramp3nramp4 seedlings on low Fe. In parallel to their genetic characterization, we classified the mutant candidates based on their seed Fe content and Fe localization pattern. Among them, two mutants display a pattern of Fe localization similar to the vit1-1 knockout mutant and carry mutations in the AtVIT1 gene. This result establishes a functional link between Fe loading in vacuoles by AtVIT1 and its retrieval by AtNRAMP3 and AtNRAMP4. Other mutants with distinct Fe localization patterns and contents are currently under investigation.
PCP1 AND ATOSA1: PROTEINS INVOLVED IN CHLOROPLAST IRON HOMEOSTASIS IN ARABIDOPSIS. Anna Manara, Giovanni Dal Corso, Antonella Furini Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona (Italy) Keywords: Activity-of-bc1-complex family, chloroplast, Fe This work is aimed at investigating the involvement in metal homeostasis of two Arabidopsis thaliana proteins belonging to the Activity-of-bc 1 -complex family. The first, PCP1, is homologous to a Brassica juncea gene modulated upon cadmium treatment; the second, AtOSA1, is a stress-related protein involved in plant response to oxidative stress. With the exception of a pale-green phenotype and a reduced total chlorophyll content in atosa1 and atosa1/pcp1 mutants, knock-out plants do not show any morphological/developmental abnormalities under standard conditions. Lack of PCP1 and AtOSA1 have no effects on photosynthetic performances, even though both are localised in chloroplasts. No differences in PSI and PSII protein composition were observed in mutants, with the exception of a reduction of Fe-containing Rieske and Cyt f proteins. These differences suggest that mutant plants could be impaired in homeostasis of metal ions integrated into the photosynthetic apparatus. Indeed, mutant plants present a decrease in thylakoid Fe content in comparison to WT and showed a reduced growth and pronounced leaf chlorosis when cultured under Fe deficiency. Conversely, plants overexpressing PCP1 or AtOSA1 have an increased Fe content in thylakoids. In WT plants the expression of both genes is reduced by Fe deficiency. These data indicate a possible involvement of PCP1 and AtOSA1 proteins in chloroplast Fe homeostasis.
Page 1: PROCEEDING OF
Page 4 and 5: INTERNATIONAL SCIENTIFIC COMMITTEE:
Page 6 and 7: Second Annual Conference and MC Mee
Page 8 and 9: 16:30 16:30- Zeshan Hassan, Sangita
Page 10 and 11: processes 16:10 Coffee break 16:30
Page 13 and 14: HOW TO INFLUENCE THE CD CONTENT IN
Page 15 and 16: ZN UPTAKE BY WHEAT IN THE PRESENCE
Page 17 and 18: NANOSIMS: A TECHNIQUE FOR SUBCELLUL
Page 19 and 20: IN VITRO MINERAL AVAILABILITY IN PE
Page 21 and 22: RECENT ADVANCES IN COMPARTMENTATION
Page 23 and 24: production, breeding and processing
Page 25 and 26: SUBSTANTIAL NATURAL VARIATION IN MI
Page 27 and 28: ADAPTIVE MECHANISMS OF HYPERACCUMUL
Page 29 and 30: BREEDING CROPS FOR BETTER NUTRITION
Page 31 and 32: Fig 1, 190x showing biochar’s var
Page 33: V. SIMULTANEOUS BIOFORTIFICATION OF
Page 37 and 38: FROM ENZYMATIC BROWNING IN FRUITS A
Page 39 and 40: PROGRESS IN THE UNDERSTANDING OF CA
Page 41 and 42: THE ROLE OF BIOCHAR IN THE PHYTOREM
Page 43 and 44: PHYSIOLOGICAL CHARACTERIZATION AND
Page 45: ARSENIC IN RICE: GASTROINTESTINAL B
Page 49 and 50: SALINITY AND MYCORRHIZAL SYMBIOSIS:
Page 51 and 52: ALIMURGIC HERBS AS POTENTIAL SOURCE
Page 53 and 54: EFFECTS OF BIOCHAR AND GREENWASTE C
Page 55 and 56: UPTAKE OF ARSENIC INTO TOMATO PLANT
Page 57 and 58: EFFECTS OF SELENIUM ON THE GROWTH P
Page 59 and 60: EFFECT OF ADDITION OF SULPHUR FERTI
Page 61 and 62: I. SIMULTANEOUS BIOFORTIFICATION OF
Page 63 and 64: EFFECTS OF BIO-(PHYTO-, FUNGI) GENI
Page 65 and 66: ASSESSMENT OF HEAVY METAL (PB, CD,
Page 67 and 68: ZINC FLUXES INTO THE DEVELOPING BAR
Page 69 and 70: THE PRESENCE OF COPPER (Cu 2+ ) IN
Page 71 and 72: DETECTION OF CADMIUM DISTRIBUTION I
Page 73 and 74: DETERMINATION OF POLLUTION OF DRINK
Page 75 and 76: EARLY RESPONSES IN ROOT MERISTEM OF
Page 77 and 78: BUCKWHEAT PRODUCTION ON ACID AND LO
Page 79 and 80: THE IMPACT OF HEAVY METALS ON RED C
Page 81 and 82: HOW TO ENHANCE NUTRIENT ASSIMILATIO
Page 83 and 84: CONTROLLING CADMIUM ACCUMULATION IN
Page 85 and 86:
RESPONSES OF SORGHUM PLANTS TO STRE
Page 87 and 88:
TO BE ECO, OR NOT TO BE? - SOME ASP
Page 89 and 90:
III. SIMULTANEOUS BIOFORTIFICATION
Page 91 and 92:
TRACE ELEMENTS, GLUTATHIONE AND DIA
Page 93 and 94:
COMPOST INCORPORATION IN SOIL DECRE
Page 95 and 96:
INFLUENCE OF PUTRESCINE APPLICATION
Page 97 and 98:
POSSIBLE NICOTIANAMINE INVOLVEMENT
Page 99 and 100:
NUTRITIONAL QUALITY OF SELECTED SPR
Page 101:
HOW DOES THE GREEN REVOLUTION AFFEC
Page 105 and 106:
Aarts Mark; Lab. of Genetics, Wagen
Page 107 and 108:
Lacatusu Radu; National Research In
Page 109:
Schwitzguebel Jean-Paul; EPFL, Labo
show all

Book with abstracts from the COST Action 0905 meeting in ... - UMB

Create successful ePaper yourself

Delete template?

Save as template?