Book with abstracts from the COST Action 0905 meeting in ... - UMB

V. SIMULTANEOUS BIOFORTIFICATION OF Zn AND Fe IN
Triticum aestivum L. – SEED NUTRITIONAL EVALUATION
António Eduardo Leitão 1 , Maria Paula Duarte 2 , Maria Manuela Abreu da Silva 3 , Benvindo
Maçãs 4 , José Prates Coutinho 4 , Ana Sofia Almeida 4 , Ana Luísa Fernando 2 , Paula Scotti-
Campos 4 , Isabel P. Pais 4 , José Cochicho Ramalho 1 , Luis Filipe Goulão 2 , Ana Isabel
Ribeiro 1 , Fernanda Simões 4 , Ana Rita Costa 4 , José Matos 4 , Fernando Henrique Reboredo 2 ,
Fernando Cebola Lidon 2
1 Centro de Ecofisiologia, Bioquímica e Biotecnologia Vegetal, Instituto de Investigação
Científica Tropical, Quinta do Marquês, 2784-505 Oeiras, Portugal.
2 DCTB, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da
Torre, 2829-516 Caparica, Portugal.
3 ESE Almeida Garrett, Grupo Universidade Lusófona, COFAC, Palácio de Santa Helena,
Largo do Sequeira nº 7, Lisboa, Portugal.
4 Instituto Nacional de Recursos Biológicos, Estrada de Gil Vaz, Apartado 6, 7350-591
Elvas,/ Estrada do Paço do Lumiar, 22 Ed. 5, 1649-038 Lisboa/ URGEMP, Quinta do
Marquês, 2784-505, Oeiras, Portugal.
Keywords: Glicids, Nutritional value, Proteins, Triticum biofortification.
Certified seeds of Triticum aestivum L. cv Nabão (0Ts) were grown in walk-in growth
chamber (EHHF10000, Aralab, Portugal), under environmental controlled conditions (80%
RH; 24/20ºC day/night temperatures; PPFD of ca 700 µmol m -2 s -1 , 12 h photoperiod), in
pots irrigated with a standard solution (1Ts), during 1 month following germination.
Thereafter, until the end of the reproductive phase, half of the pots kept the same irrigation
solution (1Ts), but the others were submitted to a five fold concentration of all nutrients
(1T5s). The seeds obtained from 1T5s plants were grown again under the initial standard
solution of nutrients (2Ts) and to 5, 7 and 10 times higher concentration of all the nutrients
(2T5s, 2T7s and 2T10s). It was found that, relatively to 0Ts, in the seeds of 2Ts, 2T5s,
2T7s and 2T10s, Zn concentration increased about 9.92, 8.87, 4.73 and 9.00 fold, whereas
Fe augmented 3.91, 4.95, 5.10 and 8.98 times. Moreover, the yield of the ears strongly
decreased and the seeds became shrunken in 2T7s and 2T10. In the grains, relatively to
0Ts, raffinose, sucrose and starch showed, respectively, a 1.05, 1.29 and 1.04 fold increase
in 2Ts, followed by a decrease to 96% / 86% / 76%, 66% / 59% / 53% and 45% / 35% /
47%, in 2T5s, 2T7s and 2T10s. The highest concentration of glucose and fructose was
found in 2T7s (11.3 and 15.3 fold) and 2T10s (8.0 and 14.7 fold). Relatively to 0Ts, the
contents of total protein increased 1.95, 3.01, 3.03 and 3.02 in 2Ts, 2T5s, 2T7s and 2T10s,
respectively. Moreover, the amount of organic matter reached 98.3% in 0Ts, but in 2Ts,
2T5s, 2T7s and 2T10s was about 96.9, 95.9%, 96.3% and 95.5%, respectively. Relatively
to 0Ts, the ashes in 2Ts, 2T5s, 2T7s and 2T10s showed 1.82, 2.12, 2.41 and 2.68 fold
increases. The results suggest that at a nutritional level, 2T5s is the best option for
simultaneous biofortification of Zn and Fe.