CURRICULAM VITAE - Australasian Plant Pathology Society
CURRICULAM VITAE - Australasian Plant Pathology Society
CURRICULAM VITAE - Australasian Plant Pathology Society
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Dr. K. SRINIVASAN, M.Sc., Ph.D.,<br />
<strong>CURRICULAM</strong> <strong>VITAE</strong><br />
IRCSET Postdoctoral Research Scientist<br />
Shannon Applied Biotechnology Centre<br />
Institute of Technology, Tralee, Kerry, Ireland<br />
E-mail: seenu.cas@gmail.com/srinivasan.krishnamoorthy@staff.ittralee.ie<br />
Mobile no. 00353863291293<br />
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Research Experience Summary:<br />
I have completed my Ph.D., degree in Applied Microbiology - Botany (Specialization in<br />
<strong>Plant</strong> <strong>Pathology</strong>) from July 2003 to November 2007 at Centre for Advance Studies in Botany,<br />
University of Madras, India. After that, I have worked as a Postdoctoral Fellow at Agroinnova,<br />
University of Torino, Italy from October 2007 to September 2008. I have worked as a Postdoctoral<br />
Molecular <strong>Plant</strong> Biologist at same Centre in Italy from January 2009 to September 2009.<br />
Currently, I am working as a Senior Postdoctoral Molecular <strong>Plant</strong> Biologist at Shannon Applied<br />
Biotechnology Centre, Institute of Technology, Ireland from March 2010 to June 2012.<br />
I am an expert (from 2002 to 2012) in the emerging fields of plant protection and<br />
production, plant molecular biology, plant biochemistry, plant physiology, agronomy, induced<br />
systemic resistance, plant microbial/fungal genetics, plant biotechnology and plant-microbe<br />
interactions and also with good hands-on experience in both classical and molecular techniques<br />
with bioinformatics tools.<br />
Based on my research experience, I have used more than 20 biological control agents and<br />
several commercial fungicides/pesticides to control the plant pathogens/diseases. I have handled<br />
more than 11 different crops with 10 various plant fungal and plant viral pathogens. I have good<br />
experiences in glasshouse and field based research in plant pathology including diagnosis. I have<br />
conducted 3 different field trials in India and Ireland using biocontrol agents and commercial<br />
fungicides against natural fungal and viral diseases. I have published 21 research articles in<br />
international and national journals, 21 abstracts published in national and international<br />
conferences proceedings, 2 book chapters, 8 manuscripts are in review, 89 bacterial and fungal<br />
gene sequences submitted to NCBI-USA, 11 conferences attended, 3 training programmes, 2 years’<br />
teaching experience in graduate and post-graduate levels, 6 invited lectures in different countries<br />
and received the best oral presentation award.<br />
Professional experience<br />
Awards/Scholarships/Fellowships/Membership<br />
(I)<br />
Received the IRCSET Postdoctoral Research Award (http://www.shannonabc.ie/staff.php)<br />
from Shannon Applied Biotechnology Centre, Institute of Technology, Tralee, Ireland<br />
(September 2011 to September 2012).<br />
(1) IRCSET- EMPOWER Postdoctoral Fellowship Enterprise Partnership Scheme - 'Natural<br />
Bioresources for Induction of <strong>Plant</strong> Disease Resistance', [€118,920,<br />
(http://ldrive.ittralee.ie/Research_Tab/Research_And_Enterprise/funding/successfulgrants.<br />
html) collaborated with Department of Botany and <strong>Plant</strong> Science, National University of<br />
Ireland, Galway, Ireland (www.nuigalway.ie/botany/botany_staff.html) and Brandon<br />
Products Ltd., (Collaborative Company), Tralee, Co.Kerry, Ireland,<br />
www.brandonproducts.com.index.html].<br />
(2) Enterprise Ireland - Innovation Voucher - 'Development of natural bioresources consortia<br />
for crop protection' [€6050, www.itt.ie].
(II)<br />
(III)<br />
(IV)<br />
(V)<br />
(VI)<br />
Received the Postdoctoral Research Fellowship from Shannon Applied Biotechnology<br />
Centre, Institute of Technology, Tralee, Ireland (March 2010 to August 2011).<br />
Research titles:<br />
(1): Seaweed extracts and their refined fractions, shellfish waste chitin evaluated in<br />
tomato plants against fungal diseases;<br />
(2): Investigation of defence related gene expressions on Arabidopsis thaliana using<br />
seaweed extracts;<br />
(3): Seaweed extracts and their refined fraction improves barley’s germination,<br />
growth and yield parameters.<br />
Received the Postdoctoral Research Fellowship from Agroinnova, University of Torino,<br />
Turin, Italy www.agroinnova.org. Research title: “Molecular characterization of formae<br />
speciales differentiation of pathogenic Fusarium oxysporum on vegetable crops in Italy”.<br />
Received the Postdoctoral Research Fellowship under the mutual cooperation agreement<br />
between Italian Ministry of University and Research (MIUR) and the Indian Ministry of Science<br />
and Technology (October 2007 to September 2008), Agroinnova, University of Torino,<br />
Turin, Italy www.agroinnova.org. Research title: “Biological control of Fusarium wilt on<br />
vegetable crops using different bacterial strains”.<br />
Won the Best Oral Presentation (Title: ISR mediated biological control of Sunflower<br />
Necrosis Virus Disease by using consortium of biocontrol agents) at the National Symposium<br />
on Biotechnological Methods for Crop Disease Management. Annamalai University, Annamalai<br />
Nagar, India, March 3 - 4, 2006.<br />
Member of European <strong>Plant</strong> Science Organization (EPSO), Europe from May 2010 to till date<br />
(www.epsoweb.org/membership).<br />
(VII) Member of American Phytopathological <strong>Society</strong>, USA - from August 2008 to August 2009.<br />
(VIII) Life time member of Chennai Science Club Organization, India.<br />
In Ireland:<br />
Postdoctoral Research Summary: (March 2010 – September 2012)<br />
(1): Seaweed extracts and their refined fractions, shellfish waste chitin evaluated against<br />
Fusarium wilt on tomato plants under glasshouse conditions<br />
In the present study, seaweed extracts (SEs A and B) and their refined fraction(s) (RFs I to VI),<br />
shellfish waste chitin, spent-dairy yeast β-glucans, along with their suitable commercial<br />
fungicides, pathogen treated and healthy control were used in three different inoculation methods<br />
(pre-, co- and post-inoculations) to control the Fusarium wilt caused by Fusarium oxysporum f. sp.<br />
lycopersici under glasshouse conditions. Six RFs (RFS I to VI) were extracted and quantified from<br />
seaweed extracts A and B. Results of this study, % increases of disease reduction from 7.7 to<br />
38.5%, 8.6 to 40.0% and 8.3 to 33.3% and % increases of plant height up to 1.7 to 15.7%, 0.3 to<br />
16.7% and 8.4 to 25.6% were recorded in pre-, co- and post-inoculated plants, respectively when<br />
compared to control (pathogen treated) plants. Similarly, the % increases of plant biomass up to<br />
12.7 to 81.6% and 3.6 to 31.5% were recorded in pre- and post-inoculated plants whereas there<br />
was no significant % increase of plant biomass recorded in co-inoculated plants. Among the<br />
inoculation methods tested, pre-inoculation are superior to co- and post-inoculation trials. Among<br />
the treatments evaluated, significant disease reduction were recorded by the treatments of RF I in<br />
pre-inoculation, RF I, II and IV in co-inoculation and RF I and IV in post-inoculation. RF V and SE B<br />
significantly improved the plant height in all the inoculation methods. Highest plant biomass was<br />
recorded in the treatments of SEs A and B in pre-inoculated trial. Similarly, RF III enhanced the
plant biomass in post-inoculated trial and there was no significant plant biomass recorded in coinoculated<br />
trial. Treatment of SE A significantly induced the systemic disease resistance<br />
components (peroxidise, polyphenol oxidase, phenylalanine ammonia lyase, total phenol and total<br />
protein) when challenged with inoculation of pathogen. In qRT-PCR assay, >20 to 50 fold increases<br />
of pathogen related protein (PR1) expression was observed in the RF I, SEs A and B treated plants<br />
whereas there was no pathogen related protein (PR5) and phenylalanine ammonia lyase (PAL)<br />
genes expression recorded in this study.<br />
Seaweed extracts (SEs) and their refined fractions (RFs), shellfish waste chitin and spent-dairy<br />
yeast β-glucans along with their suitable commercial fungicides against various tomato diseases<br />
such as Damping-off and Verticillium Wilt under glasshouse conditions. Some of them have shown<br />
effective disease(s) protection on tomato plants than commercial fungicide(s). The plant disease<br />
incidence and plant growth parameters like plant height and total biomass were measured. The<br />
potential SEs, RF(s) and chitin were screened for induction of systemic resistance (ISR) activity<br />
against tomato diseases. The pathogenesis related proteins (PR1, PR5 and PAL) expressions were<br />
characterized on potential SEs, RF(s) and chitin treated and control plants using qRT-PCR assay.<br />
The potential treatments of SEs, RF(s) and chitin were developed as consortia (combination of<br />
treatments) and evaluated against tomato diseases under glasshouse condition.<br />
The SEs and their RF(s) were applied as seed treatment with different concentrations in eight<br />
various vegetable seeds (i.e., barley, carrot, cucumber, grass, lettuce, potato, strawberry,<br />
tomato) to improve seed germination and plant growth promotion. Some of them have shown very<br />
effective to improve the seed germination and plant growth promotion. In addition, the seaweed<br />
extracts have evaluated against salinity and drought tolerance in barley under glasshouse<br />
conditions.<br />
(2): Investigation of defence related gene expressions on Arabidopsis thaliana using<br />
seaweed extracts<br />
In the present study, we investigated the expression of pathogenesis related protein(s) on<br />
Arabidopsis thaliana which was induced by Ascophyllum nodosum extracts (ANEs) A and B under<br />
controlled growth chamber conditions. The ANEs A and B (1:500 v/v) were applied as foliar spray<br />
to 4 weeks old A. thaliana plants (Col-6). Shellfish waste chitin applied as soil application. The<br />
sterile water treated plants served as a control. Total RNA was extracted from all the collected<br />
samples following standard methods. The RNA samples were quantified and purified for cDNA<br />
synthesis. The cDNA synthesized samples amplified with Arabidopsis specific primers such as PR1,<br />
PR5 and PDF.1 using qRT-PCR to check the defence related gene expression. The Universal<br />
primers, Actin and UBQTIN were used as a control in this study. Additionally, the micro-array<br />
analysis was carried out for all the extracted samples. Results of the present study suggested that<br />
the ANEs A and B significantly improved the growth of A. thaliana especially enhanced the leaf<br />
size, improved the number of leaves, plant height and flowering than control plants. In qRT-PCR<br />
assay, PR5 gene were expressed up to 100 fold in ANE A treated plants and >20 fold increase of<br />
PR5 gene was observed in ANE B treated plants. >50 fold increases of PR1 and PDF1 genes<br />
expression were observed in ANE A treated plants. There was no induction of PR1 and PDF1 genes<br />
observed in ANE B treated plants. In micro-array analysis, 245 and 13 genes were up regulated on<br />
A. thaliana plants by ANE A and B treatments, respectively whereas 5 similar genes are present in<br />
both treatments for up-regulation. In down regulation, 156 and 11 genes were down regulated on<br />
A. thaliana plants by ANE A and B treatments, respectively whereas 51 similar genes are<br />
commonly present in both treatments. The individual analysis of the data showed that the 250<br />
genes and 207 genes were up and down-regulated, respectively by the treatment of ANE A vs<br />
control plants. Similarly, 18 and 62 genes were up and down regulated, respectively by the<br />
treatment of ANE B vs control plants.
(3): Seaweed extracts and their refined fraction improves barley’s germination, growth<br />
and yield parameters<br />
In the present study, different concentrations of seaweed extracts (SEs) A and B and<br />
refined fractions (RFs), rich in bioactive molecules, were applied as seed treatment on barley to<br />
improve the germination and promote plant growth. Results of this study showed significant<br />
difference in seed germination and plant growth promotion in all the treatments. The highest seed<br />
germination, shoot length, root length and total biomass were recorded in the treatments of 2.5%<br />
RF III/kg of seed, 3.2% SE A/kg of seed, 2.5% RF I/kg of seed and 2.5% SE B/kg of seed. In field<br />
experiment study, seven different treatments such as 2.5 and 5.0 L/ha of SE A and B, 2.5 L/ha of<br />
RF III, farmer’s conventional practice and control were evaluated on growth and yield<br />
improvements. Treatment with SE A @ 5 L/ha improved the maximum shoot length. SE B @ 2.5<br />
L/ha significantly enhanced the plant biomass and total grain yield, respectively. Application of RF<br />
III @ 2.5 L/ha improved the 1000 seed weight, grain yield, total grain yield, additional yield<br />
(1887.1 kg/ha), additional income (311.4 €/ha) and benefit cost ratio (2.3). This study concluded<br />
that the seaweed extracts A @ 5 L/ha and B @ 2.5 L/ha and refined fractions III @ 2.5 L/ha were<br />
superior in seed germination, plant growth promotion and yield improvements.<br />
Manuscript under review from current research work<br />
1. Srinivasan, K., and O’Connell, S. 2012. Induction of systemic resistance against Fusarium<br />
Wilt on tomato plants. Phytopathology.<br />
2. Srinivasan, K., Peter, M., Antonie, F., O’Connell, S. and Spillane, C. 2012. Micro-array<br />
analysis of seaweed extracts induced gene expression in Arabidopsis thaliana. Molecular<br />
plant-microbes Interaction.<br />
3. Srinivasan, K. and O’Connell, S. 2012. Commercial seaweeds accelerate the barley growth<br />
and yield improvements in Ireland under natural field conditions. Crop Protection.<br />
4. Srinivasan, K. and O’Connell, S. 2012. Tomato disease protection using Nocardia composta<br />
SABC012. Phytopathology.<br />
5. Antonie, F., Peter, M., Srinivasan, K., O’Connell, S. and Spillane, C. 2012. Study the gene<br />
expression and regulation of Arabidopsis thaliana. Molecular plant-microbes Interaction.<br />
In Italy:<br />
Molecular characterization of formae speciales differentiation of pathogenic Fusarium<br />
oxysporum on vegetable crops in Italy (January 2009 to September 2009)<br />
Sixty-eight strains of Fusarium oxysporum isolated from wilted lamb’s lettuce and rocket<br />
plants along with eight different ATCC reference strains were examined for differences in the<br />
nucleotide sequences of the ribosomal DNA intergenic spacer (IGS) region, about 2.5 kb long in the<br />
strains analyzed. A phylogenetic (neighbour-joining) analysis was performed on the strains and<br />
permitted to identify six different phylogenetic groups, named I to VI. Most of the F. oxysporum<br />
isolates collected from wilted plants cultivated in northern Italy clustered under group I and were<br />
very similar to F. oxysporum f. sp. raphani. All the isolates, including 8 control strains, were<br />
tested for pathogenicity on lamb’s lettuce and rocket cultivated in glasshouse. Most of the isolates<br />
from northern Italy were pathogenic on lamb’s lettuce and rocket plants displayed varying degrees<br />
of virulence, and their IGS sequence showed similarity to the forma specialis raphani. In<br />
conclusion, the analysis of the IGS sequences revealed that the isolates studied had different<br />
origins and that phylogeny and pathogenicity were related; non-pathogenic isolates differed<br />
genetically from those poorly, moderately and highly virulent. This is the first report of<br />
differentiation of formae speciales of F. oxysporum on lamb’s lettuce and rocket plants by IGS<br />
sequence analysis.
Biological control of Fusarium wilt on vegetable crops using different bacterial<br />
strains (October 2007 to September 2008)<br />
The efficient plant growth promoting bacteria were isolated from disease suppressive soils<br />
and soilless crops systems. The isolated strains were screened to select the potential biocontrol<br />
agents against Fusarium wilt under greenhouse conditions. Besides, the selected biocontrol agents<br />
were identified with specific molecular tools (16S r RNA) and the classification of microbial strains<br />
(16S rRNA gene sequence of the efficient antagonistic bacterial strains was submitted in Genbank,<br />
NCBI, USA). Finally, the effective bacterial mediated biological of Fusarium wilt in different crops<br />
such as tomato, lamb’s lettuce and rocket with four different pathogenic Fusarium spp. under<br />
greenhouse conditions was achieved first time in the European continent. Five bacterial strains<br />
(FC6B, FC7B, FC8B, FC9B and FC24B) isolated from used rockwool soilless substrates were<br />
identified using 16S ribosomal DNA sequence analysis as belonging to the Pseudomonas genus.<br />
Seven glasshouse trials (Soil application: Trial I to VI; Root dipping: Trial VII) were conducted in<br />
order to evaluate the efficacy of these bacteria strains (Pseudomonas putida FC6B, Pseudomonas<br />
sp. FC7B, Pseudomonas putida FC8B, Pseudomonas sp. FC9B and Pseudomonas sp. FC24B) together<br />
with Achromobacter sp. AM1 and Serratia sp. DM1 obtained from suppressive soil, against<br />
Fusarium wilt of tomato (experiment I) and rocket (experiment II) plants. Two commercial<br />
bioproducts, Trichoderma harzianum T22 and Pseudomonas chlororaphis MA342 were also<br />
evaluated. In experiment I, the lowest disease incidence was recorded with a single application of<br />
P. putida FC6B. Similar results were obtained with the same bacteria when the concentration was<br />
decreased but an increasing number of applications were required. The highest plant biomass was<br />
recorded in the P. putida FC8B treatment. In experiment II, the lowest disease incidence was<br />
observed with the application of Achromobacter sp., Serratia sp. and Pseudomonas sp. FC9B<br />
against Fusairum wilt. Maximum plant biomass was recorded in Serratia sp. treated plants. In<br />
conclusion, the study showed the potential biocontrol activity of bacterial strains (FC6B, FC8B,<br />
Achromobacter sp. AM1, Serratia sp. DM1 and FC9B) against Fusarium wilt on tomato and rocket<br />
plants<br />
Outline of the Ph. D., thesis<br />
In India:<br />
Induced systemic resistance mediated biological control of sunflower necrosis virus<br />
disease using plant growth promoting microbial consortia (December 2002 to November<br />
2007)<br />
The research work was mainly focused on biological control of sunflower necrosis virus<br />
(SNV) using plant growth promoting microbial consortia. SNV was maintained in sunflower callus<br />
by the method of direct initiation and soak prick culture. SNV was purified by sucrose density<br />
gradient centrifugation and the SNV were maintained in pure form. The successful maintenance of<br />
SNV in tissue culture calli has been demonstrated for the first time. Purified SNV particles were<br />
visualized by Transmission Electron Microscopy. Besides, the polyclonal antibody against SNV was<br />
raised for serological diagnosis such as Ouchterlony double immuno diffusion, Enzyme Linked<br />
ImmunoSorbant Assay (ELISA) and western blot analysis.<br />
Six different BCAs (Streptomyces sp. PM5, Trichothecium roseum MML003, Bacillus<br />
licheniformis MML2501, Streptomyces fradiae MML1042, Pseudomonas aeruginosa MML2212 and<br />
Bacillus sp. MML2551) were evaluated with seed and foliar applications in this study along with<br />
Morinda pubescens fruit extract against sunflower necrosis virus disease (SNVD). Among different<br />
treatments tested, four BCAs (Bacillus sp. MML2551, P. aeruginosa MML2212, B. licheniformis<br />
MML2501 and S. fradiae MML1042) performed well against SNVD and enhancing plant growth.<br />
Therefore, they were used to develop all the possible combination of two, three and four culture<br />
consortia (11 different consortia) for further evaluation against SNVD with the ultimate goal of<br />
identifying an efficient consortium of BCAs, which would provide high level disease protection
against SNVD. In addition, Induced Systemic Resistance (ISR) following the application of BCAs<br />
with seed and soil treatments in sunflower were investigated. The components of ISR such as plant<br />
defense related enzymes viz., salicylic acid, peroxidase, polyphenol oxidase, phenylalanine<br />
ammonia lyase, tyrosine ammonia lyase, total proteins, total phenol, protease and catalase and<br />
also other parameters like reducing sugars and chlorophylls were estimated in different days<br />
intervals in three and four culture consortia of BCAs treated and control sunflower plants. This is<br />
the first report on ISR due to application of BCAs in sunflower against SNVD.<br />
The effective BCAs formulated (powder & liquid) individually with talc powder were mixed<br />
prior to application in the field experiments. These bio-formulations were evaluated as three and<br />
four culture consortia with Bl + Pa + Bsp and Bl + Sf + Pa + Bsp, respectively against SNVD in two<br />
different field experiments. The highest reduction of SNVD up to 40.9% and 51.4%, respectively<br />
were recorded due to treatment of the powder and liquid formulation of consortia (Bl + Sf + Pa +<br />
Bsp) as compared to control. In addition, The seed yield and income were increased due to<br />
treatment of four culture consortium, which registered an additional seed yield of 840 kg/ha<br />
(powder formulations) and 936 kg/ha (liquid formulations) with the profit of Rs. 10920/ha and Rs.<br />
12168/ha and an additional income of Rs.7865 and Rs.8164/ha compared to control.<br />
Education<br />
In 2007: Ph.D., Applied Microbiology - Botany (Specialization in <strong>Plant</strong> <strong>Pathology</strong>), Centre for<br />
Advanced Studies in Botany, University of Madras, Chennai, India<br />
In 2002: M. Sc., Applied Microbiology, K. K. College, Periyar University, Velur (Namakkal), India<br />
In 2000: B. Sc., Biochemistry, K. K. College, University of Madras, Velur (Namakkal), India<br />
In 2000: Post Graduate Diploma in Computer Application, Manipal University, India<br />
Publications<br />
1. Srinivasan, K. Gilardi,G. Davide, S. Gullino, M.L. and Garibaldi, A. 2012. Genetic diversity<br />
and pathogenicity of Fusarium oxysporum isolated from wilted rocket plants in Italy –<br />
Phytoparasitica, 40(2): 157-170 (IF: 0.621).<br />
2. Srinivasan, K. and Mathivanan, N. 2011. Establishment and maintenance of Sunflower<br />
Necrosis Virus (SNV) in tissue culture calli and its isolation, purification and serological<br />
diagnosis. Phytoparasitica, 39(5): 509-515 (IF: 0.621).<br />
3. Srinivasan, K. and Mathivanan, N. 2011. Liquid formulation of plant growth promoting<br />
microbial consortia evaluated against sunflower necrosis virus disease under field<br />
conditions. Journal of Biopesticides 4(1): 65-72 (IC value 9.0 in 2010 - India rating is 3.2 in<br />
2011 – ISSn (print) 0974-391X; Online 2230-8385).<br />
4. Srinivasan K., Gilardi G., Spadaro D., Garibaldi A., Gullino M.L. 2010. Molecular<br />
characterization through IGS sequencing of formae speciales of Fusarium<br />
oxysporum pathogenic on lamb’s lettuce. Phytopathologia Mediterranea 49: 309-320 (IF:<br />
1.117).<br />
5. Srinivasan, K. and S. Visalakchi. 2010. First report of Rhizoctonia solani SV001 attacking<br />
sunflower in India. <strong>Plant</strong> Disease 94(4): 488 (IF: 2.387).<br />
6. Jayaprakashvel, M., Selvakumar, M., Srinivasan, K., Ramesh, S. and Mathivanan, N. 2010.<br />
Control of sheath blight disease in rice by thermo-stable secondary metabolites of<br />
Trichothecium roseum MML003. European Journal of <strong>Plant</strong> <strong>Pathology</strong> 126(2): 229-239 (IF:<br />
2.054).
7. Srinivasan, K. and Mathivanan, N. 2009. Biological control of sunflower necrosis virus<br />
disease using powder and liquid formulations of plant growth promoting microbial consortia<br />
in field conditions. Biological Control 51(3): 395-402 (IF: 2.164).<br />
8. Srinivasan, K. Gilardi, G. Garibaldi, A. and Gullino, M.L. 2009. Bacterial antagonists from<br />
recycled soilless substrates suppress Fusarium wilt on tomato. Journal of <strong>Plant</strong> <strong>Pathology</strong><br />
91(1): 145-152 (IF: 1.054).<br />
9. Srinivasan, K. Gilardi, G. Garibaldi, A. and Gullino, M.L. 2009. Efficacy of bacterial<br />
antagonists and different commercial products against Fusarium wilt on rocket.<br />
Phytoparasitica 37(2): 179-188 (IF: 0.621).<br />
10. Srinivasan, K., Krishanraj, M. and Mathivanan, N. 2009. <strong>Plant</strong> growth promotion and the<br />
control of sunflower necrosis virus disease by the application of biocontrol agents in<br />
sunflower. Archives of Phytopathology and <strong>Plant</strong> Protection 42(2): 160-172 (IF: 0.717).<br />
11. Mathivanan, N., Surendiran, G., Srinivasan, K. and Malarvizhi, K. 2006. Morinda pubescens<br />
J.E. Smith (Morinda tinctoria Roxb.) fruit extract accelerates wound healing in rats. Journal<br />
of Medicinal Foods. 9: 591-593 (IF: 1.461).<br />
12. Srinivasan, K., Selvankumar, T. and Mathivanan, N. 2005. Isolation and characterization of<br />
M-antigen from Streptococcus pyogenes. Indian Journal of Applied Microbiology 1: 1-6.<br />
13. Mathivanan, N., Surendiran, G., Srinivasan, K., Sagadevan, E. and Malarvizhi, K. 2005.<br />
Review on the current scenario of Noni research: Taxonomy, distribution, chemistry,<br />
medicinal and therapeutic values of Morinda cotrifolia. International Journal of Noni<br />
Research 1: 1-16.<br />
Conference proceedings published in peer-reviewed journals<br />
1. Visalakchi, S., Srinivasan K. and Muthumary J. 2011. Taxol producing endophytic fungus<br />
Fusarium culmorum SVJM072 from medicinal plant of Tinospora cordifolia – a first report.<br />
Journal of Biotechnology 150S (2010) S1–S576 [P-I.222] (IF: 2.950).<br />
2. Visalakchi, S., Srinivasan K. and Muthumary J. 2011. Optimization of process parameters for<br />
improved production of Taxol by a novel endophytic fungus Pestalotiopsis oxyanthi SVJM060<br />
isolated from Taxus baccta. Journal of Biotechnology 150S (2010) S1–S576 [P-M.116] (IF:<br />
2.950).<br />
3. Spadaro D., Srinivasan K., Gilardi G., Gullino M.L., Garibaldi A. 2010. Molecular<br />
characterization through IGS sequencing of formae speciales of Fusarium<br />
oxysporum pathogenic on lamb’s lettuce and rocket. Journal of <strong>Plant</strong> <strong>Pathology</strong> 92 (4,<br />
Supplement) S4.71-S4-105 In press (IF: 1.054).<br />
4. Srinivasan K., Gilardi G., Spadaro D., Gullino M.L. Garibaldi A. 2010. Caratterizzazione<br />
molecolare di Fusarium oxysporum agenti di tracheofusariosi su valerianella. Protezione<br />
delle Colture, 3 (2), 127-128.<br />
5. Srinivasan K., Gilardi G., Spadaro D., Gullino M.L. Garibaldi A. 2010 - Diversità genetica e<br />
patogenicità di isolati di Fusarium oxysporum agenti di fusariosi su rucola in Italia.<br />
Protezione delle Colture, 3 (2), 128-129.<br />
6. Garibaldi A., Srinivasan K., Gilardi G., Gullino M.L. (2009) Effi cacia di antagonisti batterici<br />
contro la fusariosi della rucola. Protezione delle Colture, 2 (2), 64<br />
7. Srinivasan, K, Gilardi, G, Garibaldi, A. and Gullino, ML. 2008. Efficacy of various bacterial<br />
biocontrol organisms and commercial biocontrol products for control of Fusarium wilt of<br />
tomato. Journal of <strong>Plant</strong> pathology 90(3): 591 (IF: 1.054).
8. Srinivasan, K., Jayaprakshvel, M. and Mathivanan, N. 2008. Biological control of sunflower<br />
necrosis virus disease using two different formulations of plant growth promoting microbial<br />
mixtures. Journal of <strong>Plant</strong> pathology 90(2): S2.129 (IF: 1.054).<br />
Book Chapters<br />
1. Prashanth, S., Prabavathy, V.R., Malarvizhi, K., Srinivasan, K. and Mathivanan, N. 2006.<br />
Biocontrol potential of a growth promoting soil bacterium against phytopathogenic fungi.<br />
Mitt. Biol. Bundesanst-Land-Forstwirtsch. 408: 325-329.<br />
2. Gilardi, G., Srinivasan, K., Garibaldi, A. and Gullino, M.L. 2011. Efficacy of bacterial<br />
antagonists from used rockwool soilless substrates and different commercial products<br />
against Fusarium wilt on tomato. Acta Hort. (ISHS) 914:357-360.<br />
Abstracts published in Conferences/Symposiums/Seminars<br />
International<br />
1. Srinivasan, K and O’Connell, S. 2012. Seaweed extracts and their refined fractions, shellfish<br />
waste chitin evaluated against Fusarium wilt on tomato plants under glasshouse conditions.<br />
The 1 st World Congress on the use of Biostimulants in Agriculture, 26-29 November 2012.<br />
Strasbourg Congress, France (submitted).<br />
2. Srinivasan, K and O’Connell, S. 2012. Investigation of defence related gene expressions on<br />
Arabidopsis thaliana using seaweed extracts. The 1st World Congress on the use of<br />
Biostimulants in Agriculture, 26-29 November 2012. Strasbourg Congress, France<br />
(submitted).<br />
3. Sonaimuthu, V., Krishnamoorthy, S., Johnpaul, M. and Negi, AS. 2012. In Vitro assays of<br />
tenuazonic acid produced by endophytic fungus, Alternaria alternata SVJM015. Open<br />
Innovation Conference, Limerick Institute of Technology, Limerick, Ireland, 4 - 5 April 2012.<br />
4. Murphy, E., Jones, P., O’Connell, S. and Krishnamoorthy, S. 2012. Designer seaweed<br />
extracts: study of how variation in processing conditions, can effect chemical composition<br />
and bioactivity. The 10 th New Ag International Conference & Exhibition, Bangkok, Thiland,<br />
28-30 March 2012.<br />
5. Visalakchi, S., Srinivasan, K., Arvind Singh N. and Johnpaul, M. 2010. Isolation and<br />
molecular identification of novel endophytic taxol producing fungus, Pestalotiopsis oxyanthi<br />
SVJM060 from Taxus baccata. IMC9, The Biology of Fungi, Edinburgh, UK, 1 - 6 August 2010.<br />
6. Srinivasan, K, Gilardi, G, Garibaldi, A. and Gullino, ML. 2010. Efficacy of bacterial<br />
antagonists from reused rockwool soilless substrates and different commercial products<br />
against Fusarium wilt on tomato. III International Symposium on Tomato Diseases. July 25 -<br />
30, 2010, Ischia, Naples, Italy.<br />
7. Srinivasan, K., and Mathivanan, N. 2009. <strong>Plant</strong> growth promoting microbial consortia<br />
mediated biological control of sunflower necrosis virus disease under field conditions. 1 st<br />
Asian PGPR Congress, Hyderabad, India. June 21-24, 2009.<br />
8. Prashanth, S., Prabhavathy, V.R., Malarvizhi, K., Srinivasan, K. and Mathivanan, N. 2005.<br />
Biocontrol potential of a growth promoting soil bacterium against phytopathogenic fungi.<br />
First International Symposium on Biological Control of Bacterial Diseases, Darmstadt<br />
University of Technology, Darmstadt, Germany, October 23-26, 2005.<br />
9. Srinivasan, K., Surendiran, G. and Mathivanan, N. 2005. Pathological, molecular biological<br />
and biocontrol studies on sunflower necrosis virus disease by consortium of biocontrol<br />
agents. Asian Conference on <strong>Plant</strong>-Microbe Interactions, University of Madras, Chennai,<br />
India, December 8-10, 2005.
10. Surendiran, G., Srinivasan, K., Ramesh, S., Malarvizhi, K and Mathivanan, N. 2005.<br />
Antifungal activity of Morinda tinctoria against some plant pathogens. International<br />
Symposium on Integrated Management of Fungal Diseases: Technology Development and<br />
Application. Banaras Hindu University, Varanasi, India, February 24-27, 2005.<br />
11. Srinivasan, K., Peter, J. and Jayakumar, K. 2001. Impact of dual inoculation of Azospirillum<br />
sp. and Rhizobium sp. on the growth of Arachis hypogeae. Linn. International symposium on<br />
Recent Advances in Biological Sciences. Held at K.S.R. Centre for Biological Sciences K. S.<br />
Rangasamy College of Arts and Science, Tiruchengode, Tamilnadu, India, October 11-12,<br />
2001.<br />
National<br />
1. Srinivasan, K., and Mathivanan, N. 2010. Biological control of sunflower necrosis virus<br />
disease by plant growth promoting microbial consortia under field conditions. National<br />
Conference on Frontiers in Advance <strong>Plant</strong> <strong>Pathology</strong>, (March 11-12, 2010) organized by CAS<br />
in Botany, University of Madras, Chennai, TamilNadu, India.<br />
2. Srinivasan, K., Gilardi, G., Spadaro, D., Gullino, M.L., and Garibaldi, A. 2010. Genetic<br />
diversity and pathogenicity of Fusarium oxysporum isolated from vegetable crops in Italy.<br />
National Conference on Frontiers in Advance <strong>Plant</strong> <strong>Pathology</strong>, (March 11-12, 2010) organized<br />
by CAS in Botany, University of Madras, Chennai, TamilNadu, India.<br />
3. Srinivasan, K., and Mathivanan, N. 2010. Efficacy of plant growth promoting microbial<br />
consortia against sunflower necrosis virus disease under field conditions. National<br />
Conference on Frontiers in <strong>Plant</strong> Molecular Biology, (February 18-20, 2010) organized by<br />
Department of <strong>Plant</strong> Molecular Biology, University of Bharathidasan, Triuchirapalli,<br />
TamilNadu, India.<br />
4. Srinivasan, K., Gilardi, G., Spadaro, D., Gullino, M.L., and Garibaldi, A. 2010. Genetic<br />
diversity and pathogenicity of Fusarium oxysporum isolated from wilted rocket plants in<br />
Italy. National Conference on Frontiers in <strong>Plant</strong> Molecular Biology, (February 18-20, 2010)<br />
organized by Department of <strong>Plant</strong> Molecular Biology, University of Bharathidasan,<br />
Triuchirapalli, TamilNadu, India.<br />
5. Srinivasan, K., and Mathivanan, N. 2008. Biological Control of Sunflower Necrosis Virus<br />
Disease using Liquid Formulations of <strong>Plant</strong> Growth Promoting Microbial Consortia 363p. AMI<br />
Association of Microbiologists of India, 49th Annual conference International symposium on<br />
Microbial Biotechnology: Diversity, Genomics and Metagenomics (November 18-20, 2008)<br />
organized by Department of Zoology, North campus, AM 557.<br />
6. Srinivasan, K., Jayaprakshvel, M. and Mathivanan, N. 2007. Biological control of sunflower<br />
necrosis virus disease by using <strong>Plant</strong> growth promoting microbial consortia. 8 th Agricultural<br />
Science Congress, Tamil Nadu Agricultural University (TNAU), Coimbatore, India, February<br />
15-17, 2007.<br />
7. Surendiran, G., Srinivasan, K., Sagadevan, E. and Mathivanan, N. 2007. Antidiabetic,<br />
hepatoprotective and cardioprotective properties of Morinda pubescens J.E. Smith. National<br />
Conference on Recent trends on Medicinal <strong>Plant</strong>s Research. University of Madras, Chennai,<br />
India. January 24-25, 2007.<br />
8. Mathivanan, N., Selvakumar, M., Jayaprakashvel, M., Ramesh, S. and Srinivasan, K. 2006.<br />
Biological control of sheath blight disease in rice by Trichothecium roseum. <strong>Plant</strong> Disease<br />
Management Conference, Kerala, India, November 27-28, 2006.<br />
9. Srinivasan, K. and Mathivanan, N. 2006. ISR mediated biological control of sunflower<br />
necrosis virus disease by using consortium of biocontrol agents. National Symposium on
Biotechnological Methods for Crop Disease Management. Annamalai University, Annamali<br />
Nagar, India, March 3-4, 2006.<br />
10. Surendiran, G., Srinivasan, K. and Mathivanan, N. 2006. Antioxidant activity of Morinda<br />
pubescens. National Symposium on Molecular Basis of Disease Management. University of<br />
Madras, Chennai, India. March 13-14, 2006.<br />
Technical proficiency in<br />
Microbiological skills - Isolation, identification and maintenance of microorganisms (Enrichment<br />
culture technique) and Electron microscopical analysis.<br />
Analytical technical skills - Enzyme assay (Quantitative & Plate assay), Extraction of bioactive<br />
compounds from microorganisms, Purification of secondary metabolites from microbial strains by<br />
chromatographic techniques (Column, Thin layer, HPLC FPLC and Gas chromatographic<br />
techniques) and Estimation of growth hormones from plants.<br />
<strong>Plant</strong> pathology technical skills - Biological control of plant pathogens e.g., bacteria, fungi and<br />
virus, Induced systemic resistance enzyme assays, Systemic acquired resistance enzyme assays,<br />
Formulation of biocontrol agents (powder and liquid formulations), Pathogenicity assay,<br />
Glasshouse and Field trials, <strong>Plant</strong> tissue culture, <strong>Plant</strong> transformation and plant regeneration,<br />
Virus maintenance in tissue culture calli, Protoplast isolation, regeneration and fusion - fungi &<br />
plants.<br />
Serological diagnostic methods - Antibody production, Double immuno diffusion test, Enzyme<br />
linked immune sorbent assay and Western blotting.<br />
Molecular biological techniques - Isolation and purification of nucleic acids (DNA and RNA from<br />
bacteria, fungi, actinomycetes and plants), Agarose gel electrophoresis, Polymerase chain<br />
reaction and Reverse transcriptase PCR techniques, DNA restriction analysis, Ligation, Cloning,<br />
Real time PCR, RAPD and RFLP analyses, Transformation and Electroporation, Gene expression,<br />
cDNA systhesis, qRT-PCR, Micro-array analysis, Sequencing analysis and Poly acryl amide gel<br />
electrophoresis (Native and SDS-PAGE).<br />
Bioinformatics tools - NCBI database, RASMOL, DNA Baser, MEGA, UPGMA, Phylogenetic analysis<br />
(neighbour joining and Maximum Parasimony), Nucleic acid and Protein sequencing (proteins and<br />
nucleic acids BLAST, pair wise and multiple sequence alignments), Clustawl analysis and TAIR.<br />
Statistical knowledge - AGRES, ANOVA & SPSS statistical analysis for biological data.<br />
Computer knowledge - Basic, Microsoft Office (MS-word, power point, excel), photo shop &<br />
AGRES AND SPSS Statistical analysis for biological data.<br />
Submitted the following gene sequences to National Center for Biotechnology<br />
Information (NCBI), USA<br />
Fungi – Inter Transcribed Spacer (ITS) sequence: 18S ribosomal RNA gene, partial sequence;<br />
internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2,<br />
complete sequence; and 28S ribosomal RNA gene, partial sequence (500 to 800 bp lengths)<br />
1. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Alternaria alternata SVJM015 from medicinal plant, Indigofera enneaphylla<br />
(Accession Number: HM003680).<br />
2. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Pestalotiopsis oxyanthi SVJM060 from medicinal plant, Taxus baccta (Accession<br />
Number: HM003681).
3. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Curvularia lunata SVJM008 from medicinal plant, Clerodendrum inerme (Accession<br />
Number: HM003682).<br />
4. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Fusarium culmorum SVJM072 from medicinal plant, Tinospora cordifolia (Accession<br />
Number: HM003683).<br />
5. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Fusarium sp. SVJM035 from medicinal plant, Magnolia grandiflora (Accession<br />
Number: HM003684).<br />
6. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Curvularia sp. SVJM019 from medicinal plant, Indigofera enneaphylla (Accession<br />
Number: HM003685).<br />
7. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Pestalotiopsis oxyanthi SVJM005 from medicinal plant, Alstonia scholaris (Accession<br />
Number: HM003686).<br />
8. Visalakchi, S., Srinivasan, K. and Muthumary. 2010. Screening of taxol producing endophytic<br />
fungus, Alternaria arborescens SVJM 042 from medicinal plant, Sterculia foetida (Accession<br />
Number: HM003687).<br />
Fungi - Intergenic spacer (IGS) sequence: 28S ribosomal RNA gene, partial sequence; 28S-18S<br />
ribosomal RNA intergenic spacer, complete sequence; and 18S ribosomal RNA gene, partial<br />
sequence (2500 to 2600 bp lengths) – totally 76 gene sequences<br />
1. Srinivasan, K., Gilardi, G., Gullino, M.L. and Garibaldi, A. 2009. Molecular characterization<br />
of pathogenic formae speciales differentiation in Fusarium oxysporum on lamb’s lettuce (37<br />
gene sequences Accession Numbers from GQ914734 to GQ914770).<br />
2. Srinivasan, K., Gilardi, G., Spadaro, D., Gullino, M.L. and Garibaldi, A. 2009. Genetic<br />
diversity and pathogenicity of Fusarium oxysporum isolated from wilted rocket plants in<br />
Italy (39 gene sequences Accession Numbers from GU001824 to GU001862).<br />
Bacteria – 16s rDNA:<br />
1. Srinivasan, K. Saravanakumar, D., Garibaldi, A and Gullino, M.L. 2008. Identification and<br />
characterization of bacterial biocontrol agents (Pseudomonas putida strain FC6B 16S rRNA<br />
gene, partial sequence) against plant pathogens (Accession Number: EU836170).<br />
2. Srinivasan, K. Saravanakumar, D., Garibaldi, A and Gullino, M.L. 2008. Identification and<br />
characterization of bacterial biocontrol agents (Pseudomonas sp. FC9B 16S rRNA gene,<br />
partial sequence) against plant pathogens (Accession Number: EU836171).<br />
3. Srinivasan, K. Saravanakumar, D., Garibaldi, A and Gullino, M.L. 2008. Identification and<br />
characterization of bacterial biocontrol agents (Pseudomonas sp. FC24B 16S rRNA gene,<br />
partial sequence) against plant pathogens (Accession Number: EU836172).<br />
4. Srinivasan, K. Saravanakumar, D., Garibaldi, A and Gullino, M.L. 2008. Identification and<br />
characterization of bacterial biocontrol agents (Pseudomonas sp. FC7B 16S rRNA gene,<br />
partial sequence) against plant pathogens (Accession Number: EU836173).<br />
5. Srinivasan, K. Saravanakumar, D., Garibaldi, A and Gullino, M.L. 2008. Identification and<br />
characterization of bacterial biocontrol agents (Pseudomonas putida strain FC8B 16S rRNA<br />
gene, partial sequence) against plant pathogens (Accession Number: EU836174).<br />
Conference/Symposium/Seminar/Workshop<br />
1. Attended the International conference on <strong>Plant</strong> pathology, Incontri Fitoiatrici 2009, Italy.
2. Problemi fitosantari delle colture ortoflorofrutticole ed evoluzione delle strategie di difesa,<br />
. Incontri Fitoiatrici 2008, Italy<br />
3. National Conference on Agro biodiversity. NIOT, Chennai, India, February 12-15, 2006.<br />
4. National Conference on Emerging Trends in Mycology, <strong>Plant</strong> <strong>Pathology</strong> and Microbial<br />
Biotechnology. Osmania University, Hyderabad, India, December 29-31, 2004.<br />
5. Indo-UK workshop, “Ethics in Medical Research”. University of Madras, Guindy Campus,<br />
Chennai, India, 11 th October 2004.<br />
6. Awareness programme on applications of radioisotopes and radiation technology for societal<br />
development (ARRTS-2004), Anna University. Chennai, India, July 13-14, 2004.<br />
7. National Symposium on Recent trends in Biology, Biodiversity and Biotechnology of Fungi<br />
(NSBBBF) and 30 th annual meeting of the mycological society of India (MSI), CAS in Botany,<br />
University of Madras, Chennai, India, Feb 5-6, 2004.<br />
8. International Symposium on “Milestones in the development of Mycology and <strong>Plant</strong><br />
<strong>Pathology</strong>” organized by CAS in Botany, University of Madras, Chennai, March 28-30, 2003.<br />
9. National Seminar on “Microbes in Peace and War” held at Sengunthar Institute of Post<br />
Graduate Study and Research in Microbiology, Tiruchengode, Feb 21-22, 2002.<br />
10. National Seminar on “Biotechnology in the New Millennium” organized by the Institute of<br />
Bioscience, Vivekanandha College of Arts and Science for Women, Tiruchengode, Feb 15-16,<br />
2002.<br />
11. National level Seminar on “Current Trends and Future Directions in Life Science” conducted<br />
by Muthayammal College of Arts and Science, Rasipuram, Jan 4-5, 2002.<br />
Training program attended<br />
1. Training program on “Diploma in Computer Applications” conducted by Manipal Institute of<br />
Computer Education (MICE), Franchise Centre – Velur, Tamilnadu, India. (Dec 1999-<br />
June2000).<br />
2. A training course in “Various Procedure of Production and Quality Control of Rabies Vaccine<br />
and DTP group of vaccines” at Pasteur Institute of India, Coonoor, Nilgiris. India. (Sep 2001).<br />
3. State level “Training on RasMol” at Bioinformatics Cell Dr. G. R. Damodaran College of<br />
Science, Coimbatore, India. (Mar 2002).<br />
Guest and Invited Lecture/Teaching/Research supervising experience<br />
1. Invited Guest Lecture on Natural Bioresources for <strong>Plant</strong> Protection at National University of<br />
Ireland, Galway, Ireland on 15 th March 2012.<br />
2. Delivered a Guest lecture on Screening of commercial seaweed extracts and their bioactive(s)<br />
for induction of systemic resistance at Shannon Applied Biotechnology Center, Institute of<br />
Technology, Tralee, Ireland on 28 nd April 2011<br />
3. Invited lecture on Molecular characterization of induced systemic resistance components by<br />
natural bioresources for plant protection at National University of Ireland, Galway, Ireland on<br />
25 th Janyary 2011<br />
4. Presented the invited guest lecture on My experience in <strong>Plant</strong> <strong>Pathology</strong> in Shannon Applied<br />
Biotechnology Center, Institute of Technology, Tralee, Ireland on 22 nd April 2010.<br />
5. Presented the invited guest lecture on Genetic diversity and pathogenicity of Fusarium<br />
oxysporum isolated from wilted vegetable crops in Italy in Department of Microbiology, K. K.<br />
College, Velur, Namakkal, Tamil Nadu, India on 31 st December 2009.
6. Presented the invited lecture on Biotechnological applications of human welfare in Inter<br />
departmental seminar competition in CAS in Botany, conducted by Botany club, CAS in<br />
Botany, University of Madras on 24 th March 2004.<br />
7. Teaching Assistant for M. Sc., students in the Centre for Advanced Studies in Botany under<br />
the “Earn While Learn Scheme” of the UGC programme.<br />
8. As a Co-Supervisor, I guided for four M. Sc., students for their dissertation research work.<br />
Details of M. Sc., dissertation supervised<br />
Title of the M. Sc. Dissertation<br />
1. Morinda pubescens J.E. Smith (Morinda tinctoria Roxb.) fruit extract accelerates wound<br />
healing in rats.<br />
2. Keratin degradation by keratinolytic fungi Penicillium fellutanam.<br />
3. Metabolites mediated biological control of sheath blight disease in rice by Trichothecium<br />
roseum.<br />
4. Degradation of pharmaceutical effluent by plasmid transferred Pseudomonas pudita.<br />
REFERENCES<br />
1. Dr. Shane O’Connell M.Sc. Ph.D.<br />
Strand Leader,<br />
Shannon Applied Biotechnology Centre,<br />
Institute of Technology,<br />
Tralee, Co-Kerry, Ireland<br />
Email: shane.oconnell@staff.ittralee.ie<br />
Mob: 00353863571394<br />
2. Professor Charles Spillane<br />
Head of Botany and <strong>Plant</strong> Science Science Foundation Ireland (SFI) Principal Investigator<br />
Botany and <strong>Plant</strong> Science, C311 Aras de Brun,<br />
National University of Ireland, Galway, Ireland<br />
Email: charles.spillane@nuigalway.ie<br />
Mob: 00353867963624<br />
3. Dr. Michael C. Hall M.Sc. Ph.D.<br />
Registrar,<br />
Institute of Technology,<br />
Tralee, County Kerry, Ireland<br />
Tel: 00353872795648<br />
Email: Michael.Hall@staff.ittralee.ie<br />
4. Dr. Davide Spadaro<br />
Assistant Professor<br />
AGROINNOVA<br />
DiVaPRA - <strong>Plant</strong> <strong>Pathology</strong><br />
University of Torino<br />
Via L. da Vinci 44 - 10095 Grugliasco (TO) – Italy<br />
E-mail: davide.spadaro@unito.it<br />
Tel.: 00390116708942
5. Dr. P. Palani<br />
Assistant Professor<br />
Center for Advanced Studies in Botany<br />
University of Madras, Guindy campus<br />
Chennai- 600 025, India<br />
E mail: palanii7@yahoo.com<br />
Tel: 00919789063823