Strychnos 1990 - 2004 - Crops for the Future
Strychnos 1990 - 2004 - Crops for the Future
Strychnos 1990 - 2004 - Crops for the Future
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Author<br />
Title<br />
Year<br />
Source title<br />
Reference<br />
Wright CW; Phillipson JD; Awe SO; Kirby GC Warhurst DC; QuetinLeclercq J;<br />
Antimalarial activity of cryptolepine and some o<strong>the</strong>r anhydronium bases<br />
1996<br />
Phyto<strong>the</strong>rapy Research<br />
10(4): 361-363<br />
Abstract<br />
Eight naturally occurring anhydronium bases and <strong>the</strong> syn<strong>the</strong>tic quaternary compound Nbmethylharmalane<br />
were tested against Plasmodium falciparum (strain K1) in vitro. Cryptolepine was<br />
found to have similar activity to that of chloroquine but alstonine, 5,6-dihydroflavopereirine,<br />
matadine, Nb-methylharmalanae, melinonine F, normelinonine F, strychnoxanthine and serpentine<br />
were found to have little activity. Cryptolepine, given orally to mice infected with Plasmodium<br />
berghei berghei was found to have moderate antimalarial activity; parasitaemia was suppressed by<br />
8 0 % a t 5 0 m g / k g / d a y .<br />
Author<br />
Title<br />
Year<br />
Source title<br />
Reference<br />
Yamada H; Minematsu Y; Nakamura T; Mise M; Fujisaki H; Oguri K<br />
Brucine as a potent inducer of cyp2b3, <strong>the</strong> third member of <strong>the</strong> cyp2b subfamily p<br />
1996<br />
Biological and Pharmaceutical Bulletin<br />
19(2): 291-293<br />
Abstract<br />
We have indicated that strychnine and brucine induce concurrently an unknown p450 (2B-UP)<br />
which cross-reacts with anti-CYP2B1 antibody (Fujisaki et al., J. Pharmacol. Exp. Ther., 268, 1024-<br />
1031, 1994). We purified ZB-UP from brucine-treated rats and characterized it in this study. The<br />
purification was achieved by solubilization with sodium cholate followed by successive<br />
chromatographic steps with omega-aminoctyl-Sepharose 4B, DEAE-Sephacel and hydroxyapatite.<br />
The minimum molecular weight of purified 2B-UP was calculated to be 48000 by sodium dodecyl<br />
sulfate-gel electrophoresis. This preparation showed no Soret peak in <strong>the</strong> ultraviolet absorption<br />
spectrum indicating absence of heme. The amino terminal sequence of ZB-UP up to <strong>the</strong> 10th residue<br />
was consistent with <strong>the</strong> deduced amino acid sequence of CYP2B3 cDNA, but did not agree with <strong>the</strong><br />
sequences of CYP2B1/2. The result strongly suggests that ZB-UP is CYP2B3. Thus, we indicated<br />
here that <strong>Strychnos</strong> alkaloid, brucine, is a potent inducer of <strong>the</strong> CYP2B3 or <strong>the</strong> closely related P450.