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Allure™ Acidix HPLC Column - Chromtech.com.au

Allure™ Acidix HPLC Column - Chromtech.com.au

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β-Estradiol,<br />

Norethindrone and<br />

Ethynyl Estradiol on<br />

Ultra C18<br />

2<br />

Application Note: LC_0118<br />

<strong>Column</strong>: Ultra C18<br />

Catalog #: 9174565<br />

Dimensions: 150 x 4.6mm<br />

Particle Size: 5µm<br />

Pore Size: 100Å<br />

Peak List:<br />

1. β-estradiol<br />

2. norethinedrone<br />

3. ethynyl estradiol<br />

Sample:<br />

Inj.: 10µL<br />

Conc.: 333µg/mL<br />

Solvent: acetonitrile:water (80:20)<br />

1<br />

3<br />

Conditions:<br />

Mobile Phase:<br />

pH 3.0 10mM<br />

ammonium formate:<br />

acetonitrile(60:40)<br />

Flow:<br />

1.0 mL/min<br />

Temp.: 30°C<br />

Det.:<br />

UV @ 230nm<br />

0 2 4 6 8 10 12 14 min.<br />

Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.<strong>com</strong>

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