Allure™ Acidix HPLC Column - Chromtech.com.au

Allure™ Acidix HPLC Column - Chromtech.com.au Allure™ Acidix HPLC Column - Chromtech.com.au

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05.10.2014 Views

Hormones on Ultra C-4 1 2 Application Note:0085 Column: Ultra C-4 Catalog #: 9102365 Dimensions: 150 x 4.6mm Particle Size: 3µm Pore Size: 100Å Peak List: 1. ß-estradiol 2. norethindrone 3. 17α-ethinylestradiol Sample: Inj.: 15µL Conc.: 333µg/mL Solvent: acetonitrile:water (70:30) 3 Conditions: Mobile Phase: 10mM ammonium formate, pH 3.0: acetonitrile (60:40, v/v) Flow: 1.2mL/min. Temp.: 30°C Det.: UV @ 230nm 0 2 4 6 8 10 12 14 min. Restek Corporation 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 Fax: 814-353-1309

Organic Acids on Application Note: LC_0140 Ultra Aqueous C18 Peak List: Sample Conc.: (mg/mL) 1 3 4 5 Column: Ultra Aqueous C18 Catalog #: 9178565 Dimensions: 150 x 4.6mm Particle Size: 5µm Pore Size: 100Å 1. Malonic Acid 500 2. Lactic Acid 500 3. Acetic Acid 1000 4. Citric Acid 1000 5. Succinic Acid 2000 6. Fumaric Acid 10 6 Conditions: Mobile Phase: 99:1 pH 2.5 50 mM Potassium Phosphate: Acetonitrile Flow: 1.5mL/min Temp.: 25°C Det.: UV @ 210nm Sample: All analytes dissolved in HPLC grade water. 2 Inj.: 10µL 0 1 2 3 4 5 min. Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.com

Organic Acids on<br />

Application Note: LC_0140<br />

Ultra Aqueous C18<br />

Peak List:<br />

Sample Conc.:<br />

(mg/mL)<br />

1<br />

3<br />

4<br />

5<br />

<strong>Column</strong>: Ultra Aqueous C18<br />

Catalog #: 9178565<br />

Dimensions: 150 x 4.6mm<br />

Particle Size: 5µm<br />

Pore Size: 100Å<br />

1. Malonic Acid 500<br />

2. Lactic Acid 500<br />

3. Acetic Acid 1000<br />

4. Citric Acid 1000<br />

5. Succinic Acid 2000<br />

6. Fumaric Acid 10<br />

6<br />

Conditions:<br />

Mobile Phase:<br />

99:1 pH 2.5 50 mM<br />

Potassium Phosphate:<br />

Acetonitrile<br />

Flow:<br />

1.5mL/min<br />

Temp.: 25°C<br />

Det.:<br />

UV @ 210nm<br />

Sample:<br />

All analytes dissolved in<br />

<strong>HPLC</strong> grade water.<br />

2<br />

Inj.: 10µL<br />

0 1 2 3 4 5 min.<br />

Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.<strong>com</strong>

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