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Allure™ Acidix HPLC Column - Chromtech.com.au

Allure™ Acidix HPLC Column - Chromtech.com.au

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Furosemide<br />

on Ultra IBD<br />

Peak List:<br />

1. uracil (marker)<br />

2. furosemide<br />

Sample:<br />

Inj.: 5µL<br />

Conc.: 1000µg/mL<br />

Solvent: acetonitrile:water<br />

(60:40, v/v)<br />

2<br />

Application Note:0071<br />

<strong>Column</strong>: Ultra IBD<br />

Catalog #: 9175565<br />

Dimensions: 150 x 4.6mm<br />

Particle Size: 5µm<br />

Pore Size: 100Å<br />

Conditions:<br />

Mobile Phase: 20mM ammonium<br />

acetate pH 4.5:<br />

acetonitrile (70:30, v/v)<br />

Flow: 2.0mL/min.<br />

Temp.: 27.0°C<br />

Det.: UV @ 280nm<br />

Impurities in Furosemide<br />

(enlargement of baseline)<br />

1 2<br />

( )<br />

1. uracil<br />

2. furosemide<br />

1<br />

0 2 4 6 8 10 12 14 min.<br />

0 2 4 6 8 10 12 14 min.<br />

Restek Corporation 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 Fax: 814-353-1309

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