Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
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11 Component Mixture of<br />
Nucleotides, Nucleosides,<br />
and Nitrogenous Bases<br />
on Ultra IBD<br />
5<br />
Application Note: LC_0128<br />
<strong>Column</strong>: Ultra IBD<br />
Catalog #: 9175565<br />
Dimensions: 150 x 4.6mm<br />
Particle Size: 5µm<br />
Pore Size: 100Å<br />
Peak List:<br />
1. CDP<br />
2. CMP<br />
3. ATP<br />
4. ADP<br />
5. Cytidine<br />
6. TMP<br />
7. AMP<br />
8. Guanine<br />
9. Guanosine<br />
10. Adenine<br />
11. Adenosine<br />
Sample:<br />
Inj.: 20µL<br />
Conc.: 38-157µg/mL each <strong>com</strong>ponent<br />
Solvent: 20mM Ammonium Acetate, pH 5.8<br />
2<br />
1<br />
3<br />
4<br />
6 7<br />
8<br />
9<br />
10<br />
Conditions:<br />
Mobile Phase:<br />
97.5:2.5 20mM<br />
Ammonium Acetate,<br />
pH 5.8:Methanol<br />
Flow:<br />
1.0mL/min<br />
Temp.: 35°C<br />
Det.:<br />
UV @ 260nm<br />
11<br />
Component: Conc. (µg/mL):<br />
1. CDP 146<br />
2. CMP 113<br />
3. ATP 117<br />
4. ADP 145<br />
5. Cytidine 155<br />
6. TMP 157<br />
Component: Conc. (µg/mL):<br />
7. AMP 125<br />
8. Guanine 36<br />
9. Guanosine 103<br />
10. Adenine 38<br />
11. Adenosine 115<br />
0 2 4 6 8 10 12 14 16 18 min.<br />
Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.<strong>com</strong>
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