Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
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Guanine, Guanosine, GTP,<br />
GDP, and GMP on Ultra IBD<br />
Peak List:<br />
1. GTP<br />
2. GDP<br />
3. GMP<br />
4. Guanine<br />
5. Guanosine<br />
Sample:<br />
Inj.: 20µL<br />
Conc.: 80-362µg/mL<br />
each <strong>com</strong>ponent<br />
Solvent: 20mM Ammonium<br />
Acetate, pH 5.8<br />
2<br />
3<br />
Application Note: LC_0131<br />
<strong>Column</strong>: Ultra IBD<br />
Catalog #: 9175565<br />
Dimensions: 150 x 4.6mm<br />
Particle Size: 5µm<br />
Pore Size: 100Å<br />
Conditions:<br />
Mobile Phase:<br />
97.5:2.5 20mM<br />
Ammonium Acetate,<br />
pH 5.8:Methanol<br />
Flow:<br />
1.0mL/min<br />
Temp.: 35°C<br />
Det.:<br />
UV @ 260nm<br />
Component: Conc. (µg/mL):<br />
1. GTP 212<br />
2. GDP 260<br />
3. GMP 362<br />
4. Guanine 80<br />
5. Guanosine 226<br />
1<br />
4<br />
5<br />
0 2.5 5 7.5 10 12.5 15 17.5 min.<br />
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