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Allure™ Acidix HPLC Column - Chromtech.com.au

Allure™ Acidix HPLC Column - Chromtech.com.au

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Guanine, Guanosine, GTP,<br />

GDP, and GMP on Ultra IBD<br />

Peak List:<br />

1. GTP<br />

2. GDP<br />

3. GMP<br />

4. Guanine<br />

5. Guanosine<br />

Sample:<br />

Inj.: 20µL<br />

Conc.: 80-362µg/mL<br />

each <strong>com</strong>ponent<br />

Solvent: 20mM Ammonium<br />

Acetate, pH 5.8<br />

2<br />

3<br />

Application Note: LC_0131<br />

<strong>Column</strong>: Ultra IBD<br />

Catalog #: 9175565<br />

Dimensions: 150 x 4.6mm<br />

Particle Size: 5µm<br />

Pore Size: 100Å<br />

Conditions:<br />

Mobile Phase:<br />

97.5:2.5 20mM<br />

Ammonium Acetate,<br />

pH 5.8:Methanol<br />

Flow:<br />

1.0mL/min<br />

Temp.: 35°C<br />

Det.:<br />

UV @ 260nm<br />

Component: Conc. (µg/mL):<br />

1. GTP 212<br />

2. GDP 260<br />

3. GMP 362<br />

4. Guanine 80<br />

5. Guanosine 226<br />

1<br />

4<br />

5<br />

0 2.5 5 7.5 10 12.5 15 17.5 min.<br />

Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.<strong>com</strong>

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