Allure™ Acidix HPLC Column - Chromtech.com.au

Allure™ Acidix HPLC Column - Chromtech.com.au Allure™ Acidix HPLC Column - Chromtech.com.au

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05.10.2014 Views

Trimethoprim and Sulfamethoxazole on Allure C18 Peak List: 1. trimethoprim 2. sulfamethoxazole 3. naphthoate Sample: Inj.: 10µL Conc.: 100µg/mL Solvent: water:methanol (1:1, v/v) 1 2 Application Note:0067 Column: Allure C18 Catalog #: 9164565 Dimensions: 150 x 4.6mm Particle Size: 5µm Pore Size: 60Å Conditions: Mobile Phase: 10mm ammonium formate, pH to 3.0 with formic acid: methanol (50:50, v/v) Flow: 1.0mL/min. Temp.: ambient Det.: UV @ 220nm 3 0 2 4 6 8 10 12 14 min. Restek Corporation 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 Fax: 814-353-1309

EPA Method 8330 Application Note:0055 Peak List: 1. HMX 2. RDX 3. 1,3,5-TNB 4. 1,3-DNB 5. NB 6. Tetryl 7. 3,4-DNT (Int. std.) 8. 2,4,6-TNT 9. 4-Am-DNT 10. 2-Am-DNT 11. 2,6-DNT 12. 2,4-DNT 13. 2-NT 14. 4-NT 15. 3-NT Inj. Vol.: 5µl Conc.: (100 ug/ml each) Solvent: mobile phase 1 2 3 4 5 6 7 8 9 10 11 12 Column: Allure C18 Catalog #: 9164575 Dimensions: 250 x 4.6 mm Particle Size: 5 µm Pore Size: 60Å Conditions: Mobile Phase: Water:Methanol (50:50 v/v) Flow: 1ml/min Temperature: ambient Detection: UV @ 254nm 13 14 15 0 5 10 15 20 25 30 35 40 minutes Restek Corporation 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 Fax: 814-353-1309

EPA Method 8330<br />

Application Note:0055<br />

Peak List:<br />

1. HMX<br />

2. RDX<br />

3. 1,3,5-TNB<br />

4. 1,3-DNB<br />

5. NB<br />

6. Tetryl<br />

7. 3,4-DNT (Int. std.)<br />

8. 2,4,6-TNT<br />

9. 4-Am-DNT<br />

10. 2-Am-DNT<br />

11. 2,6-DNT<br />

12. 2,4-DNT<br />

13. 2-NT<br />

14. 4-NT<br />

15. 3-NT<br />

Inj. Vol.: 5µl<br />

Conc.: (100 ug/ml each)<br />

Solvent: mobile phase<br />

1<br />

2<br />

3<br />

4<br />

5<br />

6<br />

7<br />

8<br />

9<br />

10<br />

11<br />

12<br />

<strong>Column</strong>: Allure C18<br />

Catalog #: 9164575<br />

Dimensions: 250 x 4.6 mm<br />

Particle Size: 5 µm<br />

Pore Size: 60Å<br />

Conditions:<br />

Mobile Phase: Water:Methanol<br />

(50:50 v/v)<br />

Flow: 1ml/min<br />

Temperature: ambient<br />

Detection: UV @ 254nm<br />

13<br />

14<br />

15<br />

0 5 10 15 20 25 30 35 40<br />

minutes<br />

Restek Corporation 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 Fax: 814-353-1309

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