Allure™ Acidix HPLC Column - Chromtech.com.au

Allure™ Acidix HPLC Column - Chromtech.com.au Allure™ Acidix HPLC Column - Chromtech.com.au

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05.10.2014 Views

Organic Acids on Allure Acidix 2 Application Note:0073 Column: Allure Acidix Catalog #: 9162565 Dimensions: 150 x 4.6mm Particle Size: 5µm Pore Size: 60Å Peak List: 1. pyridine 2. benzoic acid 3. ascorbic acid Sample: Inj.: 25µL Conc.(acids): 250µg/mL Solvent: water:acetonitrile (99:1, v/v) 1 Conditions: Mobile Phase: 10mM potassium phosphate pH 2.6 : acetonitrile (60:40, v/v) Flow: 1.2mL/min. Temp.: 27.0°C Det.: UV @ 230nm 3 0 2 4 min. Restek Corporation 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 Fax: 814-353-1309

Tricyclic Antidepressants on Allure Basix (LC/MS) Peak List: 1. desipramine 2. trimipramine Sample: Inj.: 10µL Conc.: 10µg/mL Solvent: water:methanol (1:1, v/v) 1 2 Application Note: LC_0112 Column: Allure Basix Catalog #: 9161565 Dimensions: 150 x 4.6mm Particle Size: 5µm Pore Size: 60Å Conditions: Mobile Phase: 5mM ammonium formate, pH 3:methanol (25:75, v/v) Flow: 1.0mL/min, Split ratio: 1:1 Column Temp.: ambient Det.: PE/Sciex API 150 EX Interface: Heated Nebulizer Ion Mode: negative Ion Spray Voltage: 3000V Temp.: 400°C Orifice: -40V Ring: -100V Auxiliary gas: 6000cc/min 0 1 2 3 4 5 6 7 8 9 min. Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.com

Tricyclic Antidepressants<br />

on Allure Basix (LC/MS)<br />

Peak List:<br />

1. desipramine<br />

2. trimipramine<br />

Sample:<br />

Inj.: 10µL<br />

Conc.: 10µg/mL<br />

Solvent: water:methanol (1:1, v/v)<br />

1<br />

2<br />

Application Note: LC_0112<br />

<strong>Column</strong>: Allure Basix<br />

Catalog #: 9161565<br />

Dimensions: 150 x 4.6mm<br />

Particle Size: 5µm<br />

Pore Size: 60Å<br />

Conditions:<br />

Mobile Phase:<br />

5mM ammonium formate,<br />

pH 3:methanol (25:75, v/v)<br />

Flow: 1.0mL/min,<br />

Split ratio: 1:1<br />

<strong>Column</strong> Temp.: ambient<br />

Det.:<br />

PE/Sciex API 150 EX<br />

Interface: Heated Nebulizer<br />

Ion Mode: negative<br />

Ion Spray Voltage: 3000V<br />

Temp.: 400°C<br />

Orifice: -40V<br />

Ring: -100V<br />

Auxiliary gas: 6000cc/min<br />

0 1 2 3 4 5 6 7 8 9 min.<br />

Restek Corporation 110 Benner Circle Bellefonte, PA 16823 • 814-353-1300 • 800-356-1688 • Fax: 814-353-1309 • www.restekcorp.<strong>com</strong>

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