Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
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Restek -- <strong>HPLC</strong> Methods Development & Validation Kits<br />
reduce future problems are<br />
possible. Using the right<br />
columns is the key to easier<br />
methods development.<br />
Applications Notes<br />
<strong>com</strong>ponents needs a 40-minute runtime. The<br />
analyst's productivity be<strong>com</strong>es hampered by<br />
an inefficient method and laboratory overhead<br />
increases due to wasted mobile phase, more<br />
waste disposal, and additional labor to meet<br />
the deadline. In response to the needs of<br />
today's pharmaceutical laboratories, Restek<br />
has introduced <strong>HPLC</strong> and LC/MS methods<br />
development and validation kits containing a<br />
variety of columns to resolve current chromatography problems.<br />
Commonly Asked Questions<br />
• What products are included in the kits?<br />
Each methods development kit package contains four columns (Allure C18, Allure<br />
<strong>Acidix</strong>, Allure Basix, and Ultra IBD) to provide a wide range of selectivity. The kit for<br />
<strong>HPLC</strong> methods development contains the most popular size for <strong>HPLC</strong> analysis<br />
(150x4.6mm). The LC/MS kit contains the columns most frequently used for LC/MS<br />
applications (50x2.1mm).<br />
Methods validation kits contain three columns, each packed from different lots of the<br />
stationary phase. The validation kits are available for each of Restek's four most<br />
popular phases found in the methods development kit package and in two dimensions -<br />
- 150x4.6mm for <strong>HPLC</strong> methods and 50x2.1mm for LC/MS methods.<br />
• How does Restek ensure reproducibility?<br />
To ensure that Restek's phases are reproducible, a minimum of three lots of a<br />
stationary phase are verified for consistency before any new <strong>HPLC</strong> column is<br />
<strong>com</strong>mercially released. Each lot of material must meet stringent chromatographic<br />
reproducibility requirements before release, and each column is individually tested<br />
before shipment.<br />
• How do the different phases in the kits affect selectivity?<br />
The Allure C18 phase, Ultra IBD phase, Allure Basix phase, and the Allure<br />
<strong>Acidix</strong> phase provide a wide range of selectivity based upon the pKa or polarity of the<br />
analyte. The Allure C18 phase is ideal for retention of neutral, strongly lipophilic<br />
analytes. The high 27% carbon load of the Allure C18 phase results in maximum<br />
retention of lipophylic analytes.<br />
The IBD phase has many of the separation characteristics of a C18 phase and will<br />
behave much like a C18 phase in reverse phase mode, although selectivity often will<br />
be enhanced. The IBD phase's special functionality also can create additional retention<br />
http://www.restekcorp.<strong>com</strong>/hplc/methodkits.htm (2 of 3) [12/7/01 8:59:52 PM]
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