Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
Allure⢠Acidix HPLC Column - Chromtech.com.au
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RAS[TM] -- Allure[TM] Basix <strong>HPLC</strong> <strong>Column</strong><br />
NEW Allure Basix <strong>HPLC</strong> <strong>Column</strong><br />
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Maximum retention of basic (amino-containing) <strong>com</strong>pounds<br />
Increased LC/MS sensitivity<br />
Excellent general purpose column<br />
Highly reproducible and stable<br />
<strong>HPLC</strong> <strong>Column</strong>s<br />
High Performance Liquid Chromatography/Mass Spectrometry (LC/MS) has developed into one of the<br />
most powerful analytical techniques available. Although the mass spectrometer can, in itself, provide<br />
enhanced selectivity, quality chromatographic separations are essential in order to garner the full<br />
advantages of the technique. Among the advantages of LC/MS technology is an increased level of<br />
sensitivity <strong>com</strong>pared to that provided by traditional ultraviolet (UV) detection. The sensitivity achieved<br />
in the LC/MS system is related to the efficiency of the ionization/evaporation process. A greater<br />
percentage of organic modifier in the mobile phase improves the ionization/evaporation efficiency and<br />
thus provides increased sensitivity.<br />
The Allure family of <strong>HPLC</strong> columns has been designed to provide the retention and resolution<br />
required for successful LC/MS method development. Specifically, the Allure Basix phase has been<br />
engineered to produce maximum retention of basic (amino-containing) <strong>com</strong>pounds using mobile phases<br />
with high organic percentages. For LC/MS applications, this unique ability translates into increased<br />
sensitivity for the target analytes. Therefore, the Allure Basix phase is an excellent column choice for<br />
pharmaceutical applications ranging from raw material analysis to metabolic studies.<br />
APPLICATIONS<br />
Figure 1 depicts the increased retention of the basic analyte amitriptyline at high organic percentages, as<br />
<strong>com</strong>pared to the Allure C18 phase. At lower percentages of organic, hydrophobic interactions prevail<br />
and the C18 phase provides greater retention. As shown in Figure 2, the increase in retention at high<br />
organic content invokes polar interactions as the dominant separation mechanism, and produces<br />
significantly enhanced signal-to-noise (S/N) in the mass spectrometer -- nearly a 250% increase!<br />
Figure 1: Allure Basix <strong>Column</strong> Provides Increased Retention of Basic Compounds at<br />
http://www.restekcorp.<strong>com</strong>/basix/basix.htm (1 of 3) [12/7/01 8:56:47 PM]
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