Restek -- Product Instruction Sheets - Chromtech.com.au

Restek -- Product Instruction Sheets 

Product Instruction Sheets: 

GC Accessories 

● Using Polyimide Resin 

● Capillary & Micorbore Vu-Union® Connectors 

● Y Vu-Union® Connector 

● ECD/FID Make-up Gas Kit 

● Split/Splitless Capillary Injection Port for the HP 5890 GC 

● Dual Column 1/8" Inlet Fitting Kit for HP GCs 

● MSD Conversion Fitting 

● Dual Column Injection 1/8" Inlet Kit 

● Low Volume Injector (for HP 5890) 

● Low Volume Injector (for Varian) 

● Leak Detective electronic leak detector 

● QC+ Purifier Panel 

● Thermal Gas Purifier 

● Indicating Oxygen Trap 

● High Capacity Oxygen Trap 

● Indicating Glass Moisture Trap 

● Hydrocarbon Trap 

● Split Vent Trap Kit 

● 

● 

● 

● 

● 

● 

● 

● 

GC Columns 

GC/MS ANALYSIS INSTRUCTIONS 

Silcosteel® Packed Columns 

Rt-QPLOT Capillary Column 

Integra-Guard Capillary Column 

Packed Column Mesh Size Conversions 

0.25mmID/0.25µm df FAMEWAX Capillary Column 



Sample Preparation 

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Restek -- Product Instruction Sheets 

● 

SPE Sample Preparation Steps 

● 

Reference Materials 

209 Chlorobiphenyl Congeners (PCBs) 

Copyright © 1998 Restek Corporation. All Rights Reserved. 

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Restek Product Instruction Sheet -- Polyimide Resin 

Polyimide Resin 

[#56-001C - Rev. 2/95] 

Cat.# 20445 || 350°C Maximum Operating Temperature 

OVERVIEW 

Using polyimide resin with a Press-Tight® connector is not essential, however, it can assure a more 

permanent seal. If the seal between the capillary column end and the Press-Tight® connector is not 

correct initially, it will result in a leaky connection. The polyimide resin does not correct this problem; 

instead the leaky connection causes the resin to bubble and sputter during the curing stage. Therefore, it 

is critical to inspect the connection after using the resin. A proper connection exhibits a continuous 

brown ring where the column is compressed by the radial restriction of the Press-Tight® connector. 

(Refer to the Press-Tight® connector instruction sheet for the proper procedure for using Press-Tight® 

connectors.) 

INSTRUCTIONS 

1. Lightly score the tubing with a blade type scoring device (cat.# 20115 or 20116), but do not break 

it. 

2. Apply a thin layer of sealing resin by wiping the column end with a laboratory wipe containing a 

small amount of polyimide resin. 

3. Holding the column end in a upright position, lightly tap the column to remove the end portion. It 

should break easily. Inspect the cut. If the tubing does not break or the cut is not uniform, cut 

away the wiped section of tubing and repeat steps 1 and 2. 

4. Gently insert the column end into the Press-Tight® connector until it is firmly gripped in the 

radial restriction. Be careful not to press too hard or the column end will crush. A continuous 

brown ring where the column end compresses inside the connector indicates a proper connection. 

5. Follow the same procedure for inserting the other column end into the Press-Tight® connector. 

6. Leak check connections using an electronic leak detector (cat.# 21607) before the column is 

heated. 

7. Allow the connection to air dry for 30 minutes. Establish carrier gas flow through the column. 

8. Heat the column by programming the GC oven from 40°C to 150°C at 4°C/min. Hold for 30 

minutes. Then program to 220°C at 1°C/min. with a final hold of 30 minutes. 

9. Periodically leak check the connections to ensure sealing integrity. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the 

Polyimide Resin or any other Restek product. 

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Restek Product Instruction Sheet -- Polyimide Resin 

Restek Australian Distributor - Chromalytic Technology Pty Ltd (03) 9762 2034 Fax (03) 9761 1169 


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Restek Product Instruction Sheet -- Capillary & Minibore Vu-Union® Connectors 

Capillary & Minibore Vu-Union® 

Connectors 

[#56-004F - Date 11/95] 

Capillary Vu-Union® Connector: cat.# 20418 

Capillary Vu-Union® Glass Inserts: cat.# 20419 

Minibore Vu-Union® Connector: cat.# 20416 

Minibore Vu-Union® Glass Inserts: cat.# 20417 

OVERVIEW 

* To eliminate column breakage, "S" Hooks have been provided to hang the connector on the cage 

anywhere it is desired. 

The Vu-Union® is essentially a Press-Tight® that uses ferrules to make a secondary seal between the 

column and connector. A reverse ferrule design is utilized, preventing the column end from being 

crushed in the taper when the ferrule is compressed. Use only graphite ferrules since Vespel®/graphite 

ferrules will cause the insert to crack. The knurled nuts apply independent pressure to each ferrule to 

make a leak-free seal with the column end. The window allows analysts to observe the presence of a 

brown sealing ring at the column and glass insert connection, providing visual confirmation of the seal. 

INSTRUCTIONS 

1. Remove the end cap from the Vu-Union® body. Insert the tapered glass piece and replace the end 

cap. 

2. Unscrew both knurled nuts from the fitting ends. 

3. Slide both knurled nuts and ferrules onto the fused silica column ends. Install the ferrule so the 

tapered end faces outwards toward the nut. Always point the column end downward so ferrule 

shards do not fall inside the column bore. 

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4. Cut both column ends using a sapphire scribe (cat.# 20115) or ceramic scoring wafer (cat.# 

20116). Restek Australian Pointed scoring Distributor devices - Chromalytic are not Technology recommended. Pty Ltd (03) Verify 9762 a 2034 square Fax (03) cut 9761 by using 1169 a pocket 

magnifier (cat.# 20124) to inspect the end of the tubing. A square cut must be obtained or poor 

connection between the column end and the tapered glass insert will result. 

5. Push the column ends through the Vu-Union® body and partially into the glass insert taper. 

(Leave a 1/16-inch gap between the column end and the tapered region until the ferrules are 

preseated.) 

Preseat the ferrule before inserting the column completely into the taper to alleviate the 

possibility of column or tapered insert damage from the excessive force used when 

tightening the fitting. To preseat the ferrule, finger-tighten the knurled nuts until the 

column does not slide when gentle pressure is applied. The ferrules are now preseated and 

properly deformed to fit the connector cavity. 

6. Loosen both knurled nuts and gently push each column end into the glass insert taper until it 

makes contact. Tighten both knurled nuts simultaneously until the column is held firmly 

(approximately 1/8 turn past finger-tight). Look for the appearance of a brown ring at the point 

where the column ends contact the glass taper. The presence of a uniform brown ring circling the 

entire column at the tapered region indicates a proper connection. Remove and clean the glass 

insert if the column appears fractured or if the ring is not continuous. 

7. Restore carrier gas pressure and leak check the connection with a Restek Leak Detective (110 

volt: cat.# 21607, or 220 volt: cat.# 21609). Do not use liquid leak detectors because they will 

contaminate and damage the column. 

8. Inject methane to set the proper dead time. Examine the peak symmetry. If the peak is tailing, 

reinstall the connector. 

9. Next, inject 1.0µl of a solvent such as methylene chloride. If the peak exhibits the same shape as 

prior to the Vu-Union® Connector being installed, then the connection has been successfully 

achieved. Otherwise, reinstall the column into the connector. 

10. Briefly condition the column. Check the Vu-Union® Connector for leaks after the cool-down 

cycle. 

Press-Tight and Vu-Union (U.S. Pat. No. 5,288,113) are registered trademarks of Restek Corporation. 

Vespel is a trademark of E.I. DuPont Nemours & Company. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Vu- 

Union® Connector or any other Restek product. 



Restek Australian Distributor - Chromalytic Technology Pty Ltd (03) 9762 2034 Fax (03) 9761 1169 



Restek Product Instruction Sheet -- Y Vu-Union® Connector 

Y Vu-Union® Connector 

[#56-006A - Date 1/95] 

Y Vu-Union® Connector: cat.# 20432 

Capillary Tapered Glass Inserts: cat.# 20433, 20434 (3pk) 

OVERVIEW 

The Y Vu-Union® connector is essentially a Press- 

Tight® connector that uses ferrules to make a 

secondary seal between the column and connector. 

A reverse ferrule design is utilized, preventing the 

column end from being crushed in the taper when 

the ferrule is compressed. Use only graphite 

ferrules, Vespel®/graphite ferrules will cause the 

insert to crack. The knurled nuts apply independent 

pressure to each ferrule making a leak-free seal with 

the column end. Visual confirmation of a seal can 

be observed by looking for the presence of a narrow 

brown ring at the column end. 

INSTRUCTIONS 

1. Unscrew the knurled nuts and retaining rings from the main body. 

2. Place the glass insert into the main body and position the legs so they are in line with the holes in 

the main body. 

3. Carefully screw the retaining rings into the main body over the glass insert legs. This aligns the 

glass insert with the main body. 

4. Slide the knurled nuts and ferrules onto the fused silica column ends. Install the ferrule so the 

tapered end faces outwards toward the nut. Always point the column end downward so ferrule 

shards do not fall inside the column bore. 

5. Cut the column ends using a sapphire scribe (cat.# 20115) or ceramic wafer (cat.# 20116). Pointed 

scoring devices are not recommended. Verify a square cut by using a pocket magnifier (cat.# 

20124) to inspect the end of the tubing. A square cut must be obtained or poor connection 

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between the column end and the tapered glass insert will result. 

6. Push the column ends through the Y Vu-Union® connector body and partially into the glass insert 

taper. (Leave a 1/16" gap between the column end and the tapered region until the ferrules are 

preseated). 

Preseat the ferrule before inserting the column completely into the taper to alleviate the 

possibility of column or glass insert damage from the excessive force used when tightening 

the fitting. To preseat the ferrule, finger-tighten the knurled nut until the column does not 

slide when gentle pressure is applied. The ferrules are now preseated and properly 

deformed to fit the connector cavity. 

7. Loosen the knurled nuts and gently push each column end into the glass insert taper until it makes 

contact. Tighten each knurled nut until they are tight against the ferrule. Independently tighten 

each knurled nut until the column is held firmly in place. The presence of a uniform brown ring 

circling the entire column at the tapered region indicates a proper connection. Remove and clean 

the glass insert if the column appears fractured or if the ring is not continuous. 

Do not overtighten the knurled nuts or the glass will crack. 

8. Use the "S" hooks to hang the Y Vu-Union connector from the 

cage support in a manner which will not place strain against the fused 

silica tubing. 

9. Restore carrier gas pressure and leak check the connection with a Restek Leak Detective (cat.# 

21607). Do not use liquid leak detectors because they will contaminate and damage the column. 

10. Inject methane to set the proper dead time. Examine the peak symmetry. If the peak is tailing, 

reinstall the connector. 

11. Next, inject 1.0µl of a solvent such as methylene chloride. If the peak exhibits the same shape as 

prior to the Y Vu-Union® connector being installed, then the connection has been successfully 

achieved. Otherwise, reinstall the column into the connector. 

12. Briefly condition the column. Check the Y Vu-Union® connector for leaks after the cool-down 

cycle. 

Y Vu-Union® (patent pending) connector is a registered trademark of Restek Corporation. 


Contact Restek Technical Service (or an authorized distributor) if you have questions about the Y Vuhttp://www.restekcorp.com/y_vu_un.htm 

(2 of 3) [8/18/01 6:48:50 PM]


Union® Connector or any other Restek product. 

Restek Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www 


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Restek Product Instruction Sheet -- ECD/FID Make-up Gas Kit 

ECD/FID Make-up Gas Kit 

[#57-022A - Date 8/95] 

for HP GCs without make-up gas 

Make-up Gas Kit: cat.# 21300 

ECD/FID Replacement Fitting: cat.# 21324 

1. Replacement fitting (cat.# 21324) 

2. 1/4" SS nut (cat.# 21902) 

3. 1/4" Vespel®/graphite ferrule (cat.# 20221) 

4. 1/16" SS nut (cat.# 21900) 

5. 0.4mm ID graphite ferrule (cat.# 20200) 

6. Silcosteel® guide (cat.# 21302) 

7. Variable flow restrictor - not shown - (cat.# 20330) 

OVERVIEW 

This kit contains everything needed to add make-up gas to HP-5890 GCs with ECD or FID detectors. It 

is designed to function with ECDs when the Silcosteel® guide (item 6) is inserted into the hole at the top 

of the fitting and firmly pressed into place. The column end should terminate close to the outlet of the 

Silcosteel® guide. The fitting can also be used for FIDs by removing the Silcosteel guide. (The tail of the 

FID jet takes the place of the Silcosteel guide.) All column insertion distances remain the same as with 

the original equipment. Consult your GC operating manual to determine the best make-up gas flow rate 

for your detector. 

INSTRUCTIONS 

1. Decrease the detector temperature to

Restek Product Instruction Sheet -- ECD/FID Make-up Gas Kit 

4. Remove any existing make-up gas fittings or any inserts inside the 1/4" detector port. 

5. Route the 1/16" line through an existing oven hole to the variable flow outlet. If no hole exists, 

one will have to be drilled. Be sure to drill the hole in a place away from existing lines or 

electronics. Before pushing the tubing through the insulation, place a plastic cap or a piece of tape 

over the tubing end. 

6. Connect the 1/16" line to the variable flow restrictor gas outlet fitting. 

7. Insert the Silcosteel® guide (item 6). (Omit the Silcosteel guide if an FID will be used.) Connect 

the 1/4" make-up gas fitting (item 1) to the detector port using the 1/4" nut and ferrule (items 2 & 

3) provided. 

8. Cap off the column connection to the detector and leak-check all connections with Restek's Leak 

Detective (cat.# 21607) or an equivalent leak detector. After confirming that all fittings are leakfree, 

adjust the variable flow restrictor to set the make-up gas flow as indicated in the instrument 

manual. 

9. Install the column using the 1/16" nut and ferrule (items 4 & 5) according to the procedure 

outlined in your instrument manual and Restek's Capillary Column Installation Guide. 

* We highly recommend using our heat treated instrument grade SS tubing (cat.# 21512) for plumbing 

the make-up gas system to avoid the possibility of contamination. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the ECD/FID 

Make-up Gas Kit or any other Restek product. 

Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www 


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Restek Product Instruction Sheet -- Split/Splitless Capillary Injection Port for the HP 5890 GC 

Split/Splitless Capillary Injection Port for 

the HP 5890 GC 

[#57-026A - Date 10/94] 

cat.# 21625 

HP original injection port 

A. split vent gas line 

B. shell weldment 

C. inlet reducing nut 

D. retaining nut 

E. heater block 

F. split/splitless weldment 

Q. septum 

R. split/splitless weldment 

S. 1/16" swage nut 

Restek's injection port 

G. heater block 

H. shell weldment 

I. snap ring 

J. split vent gas line 

K. inlet reducing nut 

L. replaceable base screw 

M. 1/4" graphite ferrule 

N. 1/4" swage nut 

O. inlet sleeve 

P. split or splitless graphite ferrule 

INSTALLATION INSTRUCTIONS 

Before starting: 

1. Turn the main power to the instrument off and unplug the power cord from the receptacle. Allow 

adequate time for the instruments heated zones to cool down. 

2. Shut off the main source of carrier gas to the instrument. 

Removing HP's original injection port: 

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1. Remove the split vent gas line (A) which is connected to the shell weldment (B) using a 3/8" 

wrench. 

2. Remove the two screws on either side of the shell weldment (B) and pull the injector out of the 

oven. 

3. Remove the inlet reducing nut (C) and retaining nut (D) from the shell weldment (B). 

4. Carefully slide the heater block (E) off the shell weldment (B) and remove the insulation. 

5. Unscrew the split/splitless weldment (F) from the top of the injection port. Unscrew the two 

plastic finger-tight nuts that are connected to the chemical trap (carrier gas line labeled "C") and 

the three way solenoid valve (septum purge line labeled "P"). Completely remove the original 

split/splitless weldment from the GC by pulling the 1/16" tubing from the side panel. Remove the 

finger- tight nuts and o-rings from the weldment lines. Note: The plastic finger-tight nuts and o- 

rings will be reused. Do not discard. 

Installing Restek's injection port: 

1. Place the insulation from HP’s original shell weldment into the Restek shell weldment (H). 

2. Carefully insert the heater block (G) onto the Restek shell weldment (H) and place the snap ring 

(I) onto shell weldment. 

3. Place shell weldment (H) into the top of the oven and secure with original screws. 

4. Replace split vent gas line (J) onto shell weldment (H) and tighten securely. 

5. Snap the two plastic fittings onto the carrier and purge lines along with the o-rings. Reattach the 

carrier gas line (labeled "C" on the weldment) to the chemical trap. Reattach the septum purge to 

the three way solenoid valve (labeled "P" on the weldment). Route the tubing along its original 

position. 

6. Place the 1/4" swage nut (N) onto the reducing nut (K), followed by the 1/4" graphite ferrule (M). 

Next, screw in the replaceable base screw (L). Screw the reducing nut (K) assembly onto the shell 

weldment (H) base. 

7. Install the inlet sleeve (O) into top of injection port. Place a 6.35mm (split sleeve) or 6.5mm 

(splitless sleeve) ID ferrule (P) as shown over the inlet sleeve and tighten the split/splitless 

weldment (R) onto shell weldment. 

8. Place a 10 or 11mm septa (Q) into the split/splitless weldment (R) and tighten. 

9. Seal the end of the injector base fitting with the 1/16" swage nut (S) and a no-hole ferrule. Turn 

the carrier gas on and check for leaks using Restek’s Leak Detective (cat# 21607). If an 

electronic leak detector is not available, perform a pressure decay test. 

10. If no leaks are present, replace the side panel and restore power. 



REPLACEMENT PARTS 

Product 

Quantity Cat# 

Complete Injection Port Assembly 

Includes*: base fitting, split/splitless weldment, shell weldment, 

stainless steel base screw, septum nut, 1/16" and 1/4" stainless 

steel nuts, 1/4" graphite ferrule. kit 21625 

Ferrules for split sleeves (6.35mm OD): 

1/4" graphite ferrules 

1/4" Vespel®/graphite ferrules 

Ferrules for splitless sleeves (6.5mm OD) 

6.55mm ID Graphite splitless ferrules (1/4" type) 

6.55mm ID Vespel®/graphite splitless ferrule (1/4" type) 

Replacement Parts: 

Stainless Steel base screws 

Silcosteel® base screws 

gold-plated base screws 

Septum nut 

Base fitting 

Split/Splitless weldment 

Shell weldment 

10 pk. 

10 pk. 

10 pk. 

10 pk. 

10-pk. 

50-pk. 

10-pk. 

50-pk. 

2-pk. 

10-pk. 

1 

1 

1 

1 

20210 

20221 

20260 

20261 

21633 

21634 

21631 

21632 

21629 

21630 

20631 

21626 

21627 

21628 

*Does not include inlet sleeve, 1/16" type capillary ferrule, or split/splitless sleeve ferrules. Order these 

items separately. 


Split/Splitless Capillary Injection Port or any other Restek product. 

Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 - www.chromtech.net.au 



Restek Product Instruction Sheet -- Dual Column 1/8" Inlet Fitting Kit for HP GCs 

Dual Column 1/8" Inlet Fitting Kit for HP 

GCs 

[#57-019B - Rev. 2/95] 

cat.# 20645 

Replacement disk - cat.# 20392, 20393 


1. Cool GC, remove the insulation cup and unscrew existing inlet 

fitting from the injection port base. 

2. Assemble as shown below and screw tightly into the GC. 

3. Insert both columns 26mm from the bottom of the 1/8" nut. (Note: 

The column insertion distance for the 1/8" dual column inlet fitting is 

the same as the standard HP insertion distance with the column 5- 

7mm inside the sleeve.) 

4. Leak check with Restek's Leak Detective (cat.# 21607). 

*Requires special replacement disk with 1/16" hole (cat.# 20392/2-pk., 20393/10-pk.). 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Dual 

Column 1/8" Inlet Fitting Kit or any other Restek product. 

Restek Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www.chromtech.net.au 


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Restek Product Instruction Sheet -- Restek's MSD Conversion Fitting 

Restek's MSD Conversion Fitting 

[#57-027B - Rev. 1/95] 

Cat # 21314 

This MSD Conversion Fitting is used to convert your source fittings for use with standard nuts and 

ferrules. This design uses Vespel® /graphite ferrules and inexpensive 1/16" nuts instead of the costly 

instrument manufacturer's capillary nuts. The kit includes the MSD conversion fittings and aluminum 

sealing rings. 


1. Install aluminum sealing ring into the MSD adaptor fitting. 

2. Screw the MSD Conversion Fitting onto the MSD source. Be sure to use a wrench on the MSD 

source while tightening down the fitting to avoid damage to the MSD source. 

3. After installing the MSD Conversion Fitting, place a no hole ferrule in the 1/16" nut and check for 

leaks by monitoring mass 32 or 28. 

4. After the system has been certified leak free, use the appropriate Vespel® ferrule to seal the 

column. Always place a wrench on the MSD Conversion Fitting when tightening or loosening. 


Contact Restek Technial Service (or an authorized distributor) if you have questions about the MSD 

Conversion Fitting or any other Restek product. 

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Restek Product Instruction Sheet -- Restek's MSD Conversion Fitting 

Restek Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www.chromtecvh.net.au 


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Restek Product Instruction Sheet -- Dual Column Injection 1/8" Inlet Kit 

Dual Column Injection 1/8" Inlet Kit 

[#57-020A - Date 7/92] 

Cat # 20644 -- for Varian GCs 

Cat # 20645 -- for HP GCs 

This splitter guide is designed for dual column installation in Varian® or HP split/splitless capillary 

injectors. Its primary advantage is for dual 0.53mm ID column use. It utilizes standard 1/8" two-hole, 

0.8mm ferrules and 1/8" nuts. The dimensions of Restek's column splitter guide are similar to the original 

manufacturer's equipment; therefore insertion distances and operations are not changed. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about this Inlet Kit 

or any other Restek product. 



http://www.restekcorp.com/dual_kit.htm [8/18/01 6:49:49 PM]

Restek Product Instruction Sheet -- Low Volume Injector 

Restek's Low Volume Injector for HP 5890 

Split/Splitless Inlets 

[#57-028B - Date 4/95] 

(fits column IDs from 0.1mm to 0.53mm) cat.# 21692 

PRE-INSTALLATION 

1. Turn off GC and allow injector to cool. 

2. Remove the column from the injector. 

3. Remove the insulation cover and the reducing nut from inside oven. 

4. Remove the split/splitless weldment, liner, and seal from the injector. 

INSTALLATION 

1. Insert Restek's LVI into the top 

of the injector and secure with 

the 1/4" graphite or 

Vespel®/graphite ferrule and 

base nut. 

2. Install the purge & trap transfer 

line into the LVI using the 1/16" 

male nut and stainless steel 

ferrule. (Do not overtighten the 

nut as tubing bore may become 

restricted.) 

3. Install a septum plug (cat.# 

20372) and screw on the septum 

nut. 

4. Place the column nut and ferrule 

onto the column. Carefully score 

the column end with a sapphire 

scribe (cat.# 20115) or ceramic 

scoring wafer (cat.# 20116) to 

ensure a square, clean cut. Insert the column into the LVI 15mm from the bottom of the column 

nut. Using a 3/16" wrench, support the LVI and tighten the column nut 1/4 -turn past finger-tight. 

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5. Establish flow through the LVI and leak check using an electronic leak detector (cat.# 21607). 

CLEANING 

1. If adsorption or loss of resolution is observed, rinse the LVI with solvent and purge dry with 

nitrogen. 

2. Periodically change the septum to prevent small particles from entering the LVI. 


Contact Restek Technical Service (or an authorized distributor) if you have questions about the Low 

Volume Injector or any other Restek product. 

Restek Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www.chromtech.net.au | 


http://www.restekcorp.com/low_vol.htm (2 of 2) [8/18/01 6:49:57 PM]


Restek's Low Volume Injector for Varian 

Split/Splitless Inlets 

[#57-029A - Date 3/95] 

(fits column IDs from 0.1mm to 0.53mm) cat.# 21693 

PRE-INSTALLATION 

1. Turn off GC and allow injector to cool. 

2. Remove the column from the injector. 

3. Remove the injector nut, septum, septum support and liner from the injector. Remove the splitter 

guide assembly from the bottom of the injector. 

INSTALLATION 

1. Insert Restek's LVI into the top 

of the injector and secure with 

the 1/4" graphite or 

Vespel®/graphite ferrule and 

base nut. 

2. Install the purge & trap transfer 

line into the LVI using the 1/16" 

male nut and stainless steel 

ferrule. (Do not overtighten the 

nut as tubing bore may become 

restricted.) 

3. Install a septum plug (cat.# 

20372) and screw on the septum 

nut. 

4. Place the column nut and ferrule 

onto the column. Carefully score 

the column end with a sapphire 

scribe (cat.# 20115) or ceramic 

scoring wafer (cat.# 20116) to 

ensure a square, clean cut. Insert the column into the LVI 15mm from the bottom of the column 

nut. Using a 3/16" wrench, support the LVI and tighten the column nut 1/4 -turn past finger-tight. 

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5. Establish flow through the LVI and leak check using an electronic leak detector (cat.# 21607). 

CLEANING 

1. If adsorption or loss of resolution is observed, rinse the LVI with solvent and purge dry with 

nitrogen. 

2. Periodically change the septum to prevent small particles from entering the LVI. 


Contact Restek Technical Service (or an authorized distributor) if you have questions about the Low 

Volume Injector or any other Restek product. 

Restek Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www.chromtech.net.au| 


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Restek Product Instruction Sheet -- Leak Detective 

Leak Detective 

[#56-008B - Date 11/95] 

Cat.# 21607 & 21609 || NOTE: Do NOT use the Leak Detective in explosive atmospheres. 

INTRODUCTION 

The Leak Detective is Restek's 

portable leak detector specifically 

designed for use with gas 

chromatography systems. The 

Leak Detective detects minute 

leaks of any gas with a thermal 

conductivity different than air. 

The Leak Detective operates on 

two 9-volt batteries or with an AC 

adaptor. A leak is indicated by an 

LED bar graph display, as well as 

an audible tone. 

OPERATING INSTRUCTIONS 

1. Battery Installation 

Remove the battery cover and install two 9-volt alkaline batteries (included). When replacing 

batteries, always change both batteries - never mix a new battery with an old one. 

2. Powering Up 

Flip the power switch to the “ON” position. The LED display will light and the audio signal may 

sound. Adjust the “ZERO” knob until only the green LED light is lit. Allow the instrument to 

stabilize for 15-20 seconds, then zero again if necessary. 

The instrument may need to be re-zeroed periodically during use, especially if moved from roomto-room, 

or areas of different temperature or humidity. 

3. Leak Detecting 

Slowly move the probe tip around fittings and other suspected leak sources. If the Leak 

Detective senses a gas other than air, the LED bar graph will begin to light, and a tone will 

sound when the last red LED light illuminates. 

Remove the probe from the vicinity of the leak and allow the unit to return to zero. If a large 

amount of gas has entered the probe, it may take 30 seconds or more for the instrument to clear 

itself. Place the probe near the leak again to confirm its location. 

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Restek Product Instruction Sheet -- Leak Detective 

Gases with a thermal conductivity greater than air, such as hydrogen and helium, will illuminate 

the red LED lights, while gases with a lower thermal conductivity, such as CO 2 , will illuminate 

the yellow LED lights. (The audio signal sounds only when the last red LED light illuminates.) 

4. Important Notes 

Be sure fittings to be checked are clean and dry. Liquid leak detecting agents, dust, and other 

debris may damage the Leak Detective if drawn through the probe. 

The Leak Detective responds to almost anything you can smell, and many things you can't. 

Solvent vapors, split vents, or even strong air currents around the probe or reference can cause 

instability or false readings. Be careful not to breathe into the reference gas inlet when checking 

for leaks. 

Verify the operation of the Leak Detective before each use by sampling gas from a GC split 

vent, butane lighter, or other source of hydrogen or helium. 

Unstable operation and excessive flickering of the LED display indicates weak batteries. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Leak 

Detective or any other Restek product. 



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Restek Product Instruction Sheet -- QC+ Purifier Panels 

QC+ Purifier Panels 

[#59-012A - Date 12/94] 

(cat.# 21676, 21677, 21678, 21679, 21680, 21681) 

Each QC+ filter mounting head is equipped with an internal valve. The valve serves two purposes: 

1. In the up position it provides a closed gas path through the mounting head. 

2. As the valve is moved to the down position it punctures the seal on the cartridge top and shifts the 

gas flow through the cartridge. 

The QC+ has a unique cartridge that is self aligning, assuring trouble-free installation. As many as four 

cartridges can be replaced in a minute or less without contamination of your GC gas system due to lines 

being open to atmosphere during purifier replacement. 

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INSTALLATION 

INSTRUCTIONS 

1. Connect the line from the gas 

source to the fitting on the far left 

as you face the unit. Tighten the 

nut approximately 1 1/4 turns past 

finger-tight until the connection is 

leak free. Leak check using 

Restek’s Leak Detective (cat.# 

21607). 

2. With all knobs in the full up 

position, start the gas flow. Note 

the flow at the outlet fitting. 

3. Install cartridges in order noted 

on the front of the unit (moisture, 

hydrocarbon, oxygen, & 

indicating oxygen), by fitting the 

top of the cartridges in the 

mounting heads with the flat edge 

to the left. 

4. From left to right, one at a time, 

turn the knobs to the full down 

position. 

REPLACING CARTRIDGES 

1. Turn knob to the full up position. 

2. Exchange cartridges. 

3. Turn knob to the full down position. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the QC+ 

Purifier Panel or any other Restek product. 






Restek Product Instruction Sheet -- Thermal Gas Purifier 

Thermal Gas Purifier 

[#59-015A - Date: 11/95] 

THIS UNIT OPERATES AT HIGH 

TEMPERATURE. FOLLOW 

INSTRUCTIONS CAREFULLY TO AVOID 

INJURY. 

Warning: This getter reacts violently with large 

amounts of oxygen when heated. Always allow 

the getter to cool before turning off the flow of 

carrier gas to prevent oxygen back diffusion. 

Failure to observe these safety precaution could 

result in an explosion or fire. 

cat.# 21662, 21663, 21664, 21665 

1/4" Replacement Purifier Tube cat.# 21658 

1/8" Replacement Purifier Tube cat.# 21659 

Installation Instructions 

1. Select a suitable site to locate the thermal gas purifier away from flammable solvents or any heat 

sensitive materials. 

2. Cool the GC (or GCs) to ambient and disconnect carrier gas line from the rear of each instrument 

and cap the end. 

3. Cut and deburr the carrier gas line in a location appropriate to your installation allowing sufficient 

tubing length to facilitate connecting to the Thermal Gas Purifier. 

4. Release the latch on top of the purifier and open the unit. 

5. Place the "U" shaped metal getter tube into the molded heater block. 

6. Close and latch the unit. 

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7. Loosen the brass nuts on the getter tube and remove the protective plastic rods. 

8. Insert the influent and effluent carrier gas line into the brass nut on each end of the getter tube. 

The tube has no preference for carrier gas direction. Using a backup wrench on the getter tube 

tighten the brass nut 1¼ turns past finger-tight. 

9. Turn on the carrier gas to pressurize the system. Test for leaks using Restek's Leak Detective(tm) 

(110v: cat.# 21607, or 220v: cat.#21609). DO NOT USE LIQUID LEAK DETECTORS. 

10. Remove the cap from the end of the gas line(s) previously disconnected at the GC and allow the 

system to purge for 20 minutes at approximately 100 mls/minute.* 

11. Reconnect the gas line(s) to the GC. 

12. Turn on the Thermal Gas Purifier power switch. The unit will reach operating temperature within 

45 minutes and be ready for use. 

Gas Cylinder Replacement 

1. Cool the GC and turn off the power to the Thermal Gas Purifier. 

2. Close the gas line valve downstream of the regulator. 

3. Pressurize and bleed the regulator three (3) times by opening and closing the cylinder valve. After 

each pressurization bleed the gas via the carrier gas line valve.** 

4. Reconnect the carrier gas line to the regulator, turn on the gas flow and allow the system to purge 

for 20 minutes. 

5. Turn on the power to the Thermal Gas Purifier and allow 45 minutes to reach operating 

temperature. 

*It is important to purge the getter tube and associated carrier gas tubing to remove any air and moisture 

prior to heating the Thermal Gas Purifier. 

**Failure to adequately purge the regulator and the carrier gas line will reduce the life of the getter tube. 

NOTE: DO NOT USE OXYGEN OR AIR WITH THIS PURIFIER. 



Getter Tube Longevity 

The getter tube will last approximately one (1) year. 

A pressure drop will be noticed across the tube when the getter material becomes spent. When this occurs 

the tube should be changed as soon as possible to avoid a no flow condition. Downstream indicator traps 

are not recommended due to oxygen and/or moisture diffusion through the sealing mechanism of the 

various devices. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Thermal 

Gas Purifier or any other Restek product. 




Restek Product Instruction Sheet -- Indicating Oxygen Trap 

Indicating Oxygen Trap 

[#59-002D - Date: 1/96] 

1/8-inch female tube fittings - cat.# 20602 

1/4-inch female tube fittings - cat.# 20603 

Maximum Operating Pressure: 100psig || Maximum Operating Temperature: 100°C 

Caution: This trap is made of glass and can be easily broken. Pre-plumb the system by bending all metal 

tubing and aligning fittings so that minimal strain is put on the end fittings of the purifier. Always use 

two wrenches when tightening purifier fittings. Use the first wrench on the fitting body and the second 

wrench to tighten the nut. 

Installation Location 

Ideally, the trap should be installed immediately 

before the GC bulkhead carrier gas inlet fitting. 

When installed at this position, it neutralizes 

incoming O 2 leaks from prior fittings or from 

cylinder contaminants. In addition, optimal 

performance is obtained when the purifier is 

mounted in a vertical position. Vertical installation 

guards against channeling (particle settling due to 

equipment vibrations) which occurs when the trap 

is mounted horizontally allowing unpurified gas to 

reach the capillary column. 

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1. Pre-plumb the system and align all fittings 

so that minimal strain is placed on the end 

fittings of the purifier during installation. 

2. Purge gas lines at 100cc/min. flow rates for 

5 minutes. This flushes oxygen from the 

carrier gas lines. 

3. While the carrier gas is still flowing, 

quickly remove one end fitting from the 

purifier and connect it to the carrier gas 

line. Tighten the purifier fittings 

approximately 3/4 of a turn for 1/8-inch 

fittings and one full turn for 1/4-inch 

fittings. DO NOT OVERTIGHTEN OR 

THE TRAP END FITTINGS WILL 

BREAK. Some purifier inlet color change 

may occur due to unavoidable oxygen 

infiltration. 

4. Remove the other end fitting from the purifier (with the carrier gas still flowing) and carefully 

connect it to the carrier gas inlet connection of the GC as described in step 3. 

5. Leak-check with a Restek Leak Detective (cat.# 21607). 

6. The trap adsorbs incoming oxygen causing the adsorbent to change from light green (reduced 

state) to deep brown (oxidized state) in the direction of carrier gas flow. Occasionally, the purifier 

may turn deep brown at the outlet connection indicating a septum or carrier gas leak downstream 

of the purifier. Replace the trap when 90% of the adsorbent has changed color. 

Save money and waste, return your unbroken traps to us and give them a new life! 

Regenerated trap 1/4-inch fitting: cat.# 20617 

Regenerated trap 1/8-inch fitting: cat.# 20616 

Outline of the Material Safety Data Sheet 



Section I. Product Identification 

Product: 

Chemical name: 

Synonyms: 

Chemical family: 

Molecular weight: 

Formula: 

indicating oxygen adsorbent 

manganese sodium aluminosilicate 

zeolite 

molecular sieve 

not applicable 

MnO 2 , Na 2 O, Al 2 O 3 , SiO 2 

Section II. Hazardous Ingredients 

Material: sodium oxide 

CAS No.: 1313-59-3 % Wt.:


Appearance and odor - brown granules, odorless 

Section IV. Health Hazard Data 

Threshold limit value - 5mg/m 3 for respirable dust 

Effects of single (acute) overexposure: 

Swallowing: No evidence of adverse effects from available information. 

Skin absorption: No evidence of adverse effects from available information. 

Inhalation: May cause irritation of the nose and throat, accompanied by a cough and chest discomfort. 

Skin contact: May cause irritation seen as local redness. 

Eye contact: May cause irritation seen as excess redness of the conjunctiva. 

Effects of repeated (chronic overexposure): 

Prolonged inhalation may cause lung damage. 

Other effects of overexposure: none currently known. 

Medical conditions aggravated by overexposure: Breathing of dust may aggravate asthma and 

inflammation or fibrotic pulmonary disease. 

Emergency and first aid procedures: 

Swallowing: If ingested in large quantities, then drink two glasses of water. Induce vomiting if the 

patient is conscious. 

Skin contact: Wash with soap and water. 

Inhalation: Remove to fresh air. 

Eye contact: Flush eyes with water for 15 minutes. 

Note to Physician: This product is a desiccant and generates heat as it adsorbs water. The used product 

can contain material of a hazardous nature. Identify that material and treat accordingly. 

Section V. Fire and Explosion Hazard Data 

Flash point - Does not burn. 

Extinguishing media - Unused material will not burn. Use media appropriate to surrounding fire. 

Unusual fire and explosion hazards: In their fresh unused state, molecular sieves are not flammable. 

When exposed to water, however, they can get quite hot. When first wetted they can heat to the boiling 

point of water. Flooding will reduce the temperature to safe limits. 



Section VI. Reactivity Data 

Stability: stable 

Conditions to avoid: moisture (water) can cause rise in temperature which may result in burn. 

Incompatibility (materials to avoid): Sudden contact with high concentrations of chemicals having 

high heats of absorption such as olefins, HCI, etc. 

Hazardous decomposition products: The molecular sieve itself does not readily decompose unless 

subjected to extreme temperature or chemical conditions. If such decomposition did occur, the products 

would include the mix of oxides listed in Section II. 

Hazardous polymerization: Will not occur. 

Section VII . Spill or Leak Procedures 

Steps to be taken if material is released or spilled: Sweep the spill area. Collect and place the spilled 

material in a waste disposal container. Avoid raising dust. 

Waste disposal method: Discard any product including any retained materials of use, in a disposal 

container or liner in an environmentally acceptable manner, in full compliance with federal, state and 

local regulations. 

Section VIII. Special Protection Information 

Respiratory protection: Where there is excessive dustiness, wear a respirator selected as per OSHA 29 

CFR 1910.134 and approved by NIOSH/MSHA. 

Protective gloves: Use gloves impermeable to dust where prolonged hand contact is possible. 

Eye protection: Safety glasses or goggles selected as per OSHA 29 CFR 1910.133. 

Section IX. Special Precautions 

Caution: Causes eye irritation. Breathing dust may be harmful. May cause skin irritation. Open 

container slowly to avoid dust. Do not get in eyes. Avoid breathing dust and prolonged contact with 

skin. Use with adequate ventilation. Keep container closed. Wash thoroughly after handling. Do not put 

product in mouth or pour liquid into product, because burns can occur. 



Before using, you should know the hazards of the products to be absorbed on the molecular sieve. The 

products could be flammable or toxic. You should know and follow all the safety precautions related to 

the adsorbed products. 

Other handling and storage conditions: pH range if in aqueous slurry 8-11. 

Notice: The information contained in the MSDS description is applicable exclusively to the chemical 

substances identified herein and for its intended use and to the unit quantity intended for that purpose. 

The information does not relate to, and may not be appropriate for, any other applications or larger 

quantity of the substance described. 

The information has been obtained from sources believed to be reliable and accurate. Attention: This 

product in terms of chemical identity and the unit amount provided is intended for use in chemical 

analysis and not for human consumption, nor any other purpose. 

Dated 10/9/95 


Indicating Oxygen Trap or any other Restek product. 




Restek Product Instruction Sheet -- High Capacity Oxygen Trap 

High Capacity Oxygen Trap 

[#59-001C - Date: 1/96] 

1/8-inch female Swagelok fittings - cat.# 20601 




Ideally, the trap should be installed immediately before the GC bulkhead carrier gas inlet fitting. When 

installed at this position, the trap neutralizes incoming O 2 leaks from prior fittings or from cylinder 

contaminants. In addition, optimal performance is obtained when the purifier is mounted in a vertical 

position. Vertical installation guards against channeling (particle settling due to equipment vibrations) 

which occurs when the trap is mounted horizontally allowing unpurified gas to reach the capillary 

column. 


1. Pre-plumb the system and align all fittings so that minimal strain is placed on the end fittings of 

the purifier during installation. 

2. Purge gas lines at 100cc/min. flow rates for 5 minutes. This flushes oxygen from the carrier gas 

lines. 

3. While the carrier gas is still flowing, quickly remove one end fitting from the purifier and connect 

it to the carrier gas line. Tighten the purifier fittings approximately 3/4 of a turn for 1/8-inch 

fittings and one full turn for 1/4-inch fittings. 

4. Remove the other end fitting from the purifier (with the carrier gas still flowing) and carefully 

connect it to the carrier gas inlet connection of the GC as described in step 3. 

5. Leak check with a Restek Leak Detective (cat.# 21607). DO NOT use liquid leak detectors. 

6. Replace the trap after five 200 cu. ft. cylinders have been consumed. 

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Section I 


Product: CU-0226 S 14 X 20 

Common Name: 

Metal oxides on an alumina support 

For chemical emergency, call: CHEMTRAC (24 hours) 

1-800-424-9300 (US, Canada, Puerto Rico, and the Virgin Islands) 

1-202-483-7616 (outside above areas) 

Section II 

Ingredient CAS No. %Wt. 

alumina 

copper oxide 

nickel oxide 

cobalt oxide 

Section III 

1344-28-1 

1317-38-0 

1313-99-1 

1308-06-1 

80-90 

10-14 


Section IV 

Eye contact: Immediately flush eyes with plenty of water for at least 15 minutes. Call a physician. 

Skin contact: Flush with plenty of water. If irritaion persists, call a physician. 

Inhalation: Remove to fresh air. If breathing is difficult, give oxygen. Call a physician. 

Ingestion: Immediately give two glasses of water and call a physician, hospital emergency room, or 

poison control center for a way to induce vomiting. 

Section V 

NFPA Hazard Classification 

Health: 1 

Flammable: 0 

Reactivity: 0 

HMIS Hazard Classification 

Health: 2 

Flammable: 0 

Reactivity: 0 

Extinguishing media: water, carbon dioxide or foam. 

Section IX 

Specific gravity (H 2 O=1): 0.8 to 0.85g/cc 

% solubility in water: insoluble 

Section X 

Generally considered stable. 

Section XIII 

US EPA waste number: not regulated 

Section XIV 

International 

UN number: not regulated 

United States 

EPA number: not regulated 



DOT class: not regulated 

California - Proposition 65 list 

Canada 

PIN number: not regulated 

TDG class: not regulated 

EC 

DGL: not regulated 

Dated 3/18/94 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the High 

Capacity Oxygen Trap or any other Restek product. 




Restek Product Instruction Sheet -- Indicating Glass Moisture Trap 

Indicating Glass Moisture Trap 

[#59-003D - Date: 1/96] 



Maximum Operating Pressure: 125psig 

Caution: This trap is made of glass and can be easily broken. Pre-plumb the system by bending all metal 

tubing and aligning fittings so that minimal strain is put on the end fittings of the purifier. Always use 

two wrenches when tightening purifier fittings. Use the first wrench on the fitting body and the second 

wrench to tighten the nut. 


Ideally, the trap should be installed immediately 

before the GC bulkhead carrier gas inlet fitting. 

When installed at this position, the trap neutralizes 

incoming H2O from contaminated cylinders. In 

addition, optimal performance is obtained when 

the purifier is mounted in a vertical position. 

Vertical installation guards against channeling 

(particle settling due to equipment vibrations) 

which occurs when the trap is mounted 

horizontally allowing unpurified gas to reach the 

capillary column. 

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1. Pre-plumb the system and align all fittings 

so that minimal strain is placed on the end 

fittings of the purifier during installation. 

2. Purge gas lines at 100cc/min. flow rates for 

5 minutes. This flushes oxygen from the 

carrier gas lines. 

3. While the carrier gas is still flowing, 

quickly remove one end fitting from the 

purifier and connect it to the carrier gas 

line. Tighten the purifier fittings 

approximately 3/4 of a turn for 1/8-inch 

fittings and one full turn for 1/4-inch 

fittings. DO NOT OVERTIGHTEN OR 

THE TRAP END FITTINGS WILL 

BREAK. 

4. Remove the other end fitting from the 

purifier (with the carrier gas still flowing) 

and carefully connect it to the carrier gas inlet connection of the GC as described in step 3. 

5. Leak-check with a Restek Leak Detective (cat.# 21607). DO NOT use liquid leak detectors. 

6. The trap adsorbs incoming moisture causing the adsorbent to change from blue to buff in the 

direction of carrier gas flow. Replace the trap when 75% of the adsorbent changes color. 

Section I 


Product: Molecular sieve 

4A-Z8 & 13X-Z8 

Chemical Name: 

Synthetic sodium potassium or calcium aluminosilicate 



Chemical Family: 

Molecular sieve zeolite 

Section II 

Ingredient CAS No. Type 

NaA 

NaX 

68989-22-0 4A 

68989-23-1 13X 

Mg aluminosilicate 1327-43-1 clay 

Section III 

Appearance and odor: Light tan bead or powder, odorless 

Volatiles by weight: 2900°F/>1600°C 

Section IV 

Ignition temperature: non-flammable 

Section V 

Reactivity: Stable under normal temperature and pressures. 

Conditions to avoid: The addition of moisture (water) can cause rise in temperature and contact with 

skin might result in burns. 

Incompatibility: Sudden contact with high concentrations of chemicals having high heats of absorption 

such as olefins, HCI, etc. 

Section VI 

Inhalation: May cause irritation of the nose and throat, accompanied by cough and chest discomfort. 

Skin contact: May cause irritation seen as local redness and/or burns. 

Eye contact: May cause irritation seen as excess redness of the conjunctiva and/or burns. 

First Aid 

Inhalation: Remove to fresh air. 

Skin: Wash contacted area with soap and water. 



Eye: Immediately flush with plenty of water at least 15 minutes. 

Section VIII 

Protective gloves: Use gloves to avoid PROLONGED skin contact. 

Eye protection: Safety glasses or goggles selected as per OSHA 29 CFR 1910.133. 

Section IX 

The information presented herein is believed to be accurate but is not warranted. Recipients are advised 

to confirm in advance that the information is current and applicable to meet their circumstances. 

Section X 

This product contains substances which appear on the lists of indicated act or agency. 

OSHA - 29 CFR 1910 

California - Proposition 65 

IARC - Monographs on the Evaluation of Carcinogenic Risk to Humans. Volumes 1-24. 

ACGIH - Threshhold limit Values for Chemical Substance in the Work Environment. 

TSCA - 40 CFR700 

Dated 10/1/92 


Indicating Glass Moisture Trap or any other Restek product. 




Restek Product Instruction Sheet -- Hydrocarbon Trap 

Hydrocarbon Trap 

[#59-005D - Date: 1/96] 

1/8-inch tube compression fittings - cat.# 20627 

1/4-inch tube compression fittings - cat.# 20628 

carbon refill (two recharges) - cat.# 20626 



1. Remove the protective plug from one end of the trap. 

2. Locate nuts and ferrules (two each included) and insert the tubing through the nut and ferrule. 

Screw the nut onto the fitting until the tubing rests firmly against the fitting shoulder. Tighten 

finger-tight. 

3. Tighten the nut 3/4 of a turn for 1/8-inch tubing, or 1 1/4-turn for 1/4-inch tubing. 

4. Repeat steps 1 through 3 on the other end of the trap. 

5. Pressurize the system and leak-check with a Restek Leak Detective (cat.# 21607). DO NOT use 

liquid leak detectors. 

Section I 


Product: Activated carbon 

Common Name: 

Activated carbon type: KE 

Section II 

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carbon 7440-44-0 100 

Activated carbon (non-regulated) 

ACGIH, OSHA and other TLV are not applicable for activated carbon. 

Section III 

Appearance and Odor: Black granular or powder, odorless 

Specific gravity: (H 2 O=1): 1.9 to 2.2 

Packing density: (g/cc): 0.4 to 0.8 

Section IV 

Ignition temperature: (°C): 340 

Extinguishing media: Flood with plenty of water or inert gas, such as N 2 and CO 2 . 

Section V 

Hazardous decomposition: CO may be generated in the event of fire. 

Conditions to avoid: Contact with strong oxidizers, such as ozone, liquid oxygen, chlorine, 

permanganate and ketone may cause a bed fire. 

Incompatibility: Avoid contact with high concentration of ketone in air or liquid. 

Section VI 

Inhalation: Dust may be inhaled. 

Signs and symptoms of exposure: Slight irritation of eyes and nose may result from contact with carbon 

fines. 

Section VII 

First aid, eye: Flush with plenty of water at least 15 minutes. 

Section VIII 

Protective gloves: rubber 

Eye protection: safety glasses 



Respirator protection: A NIOSH approved particulate filter. 

Ventilation: Local and mechanical exhaust recommended. 

Storage and handling: Avoid generation of dust and fines during handling. 

Section IX 

Notify EPA if product spills: Report in accordance with local, state, and federal regulations. 

Cleaning procedure: Sweep up unused carbon and discard in refuse container or repackage for further 

use. 

Dated 5/93 


Hydrocarbon Trap or any other Restek product. 




Restek Product Instruction Sheet -- Split Vent Trap Kit 

Split Vent Trap Kit 

[#59-007C - Date: 1/96] 

cat.# 20640 

replacement cartridges - cat.# 20641 


1. Unscrew and remove the knurled end fitting and insert the trap cartridge. 

2. Reinstall the knurled end fitting. (The aluminum end seals of the trap are punctured as the fitting 

is tightened.) 

3. Connect the split vent trap (in either direction) to the split vent line of the gas chromatograph. 

4. Readjust the column and split vent flow rates. (The split vent trap increases the back pressure on 

the split vent outlet causing the flow rate through the column and split vent to change slightly.) 

Section I 


Product: Activated carbon 

Common Name: 

Activated carbon type: KE 

Section II 


carbon 7440-44-0 100 

Activated carbon (non-regulated) 

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ACGIH, OSHA and other TLV are not applicable for activated carbon. 

Section III 

Appearance and Odor: Black granular or powder, odorless 

Specific gravity: (H 2 O=1): 1.9 to 2.2 

Packing density: (g/cc): 0.4 to 0.8 

Section IV 

Ignition temperature: (°C): 340 

Extinguishing media: Flood with plenty of water or inert gas, such as N 2 and CO 2 . 

Section V 

Hazardous decomposition: CO may be generated in the event of fire. 

Conditions to avoid: Contact with strong oxidizers, such as ozone, liquid oxygen, chlorine, 

permanganate and ketone may cause a bed fire. 

Incompatibility: Avoid contact with high concentration of ketone in air or liquid. 

Section VI 

Inhalation: Dust may be inhaled. 

Signs and symptoms of exposure: Slight irritation of eyes and nose may result from contact with carbon 

fines. 

Section VII 

First aid, eye: Flush with plenty of water at least 15 minutes. 

Section VIII 

Protective gloves: rubber 

Eye protection: safety glasses 

Respirator protection: A NIOSH approved particulate filter. 

Ventilation: Local and mechanical exhaust recommended. 

Storage and handling: Avoid generation of dust and fines during handling. 



Section IX 

Notify EPA if product spills: Report in accordance with local, state, and federal regulations. 

Cleaning procedure: Sweep up unused carbon and discard in refuse container or repackage for further 

use. 

Dated 5/93 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Split 

Vent Trap Kit or any other Restek product. 

|Restek Australian Distributor - Chromalytic Technology Pty Ltd - Tel (03) 9762 2034 Fax (03) 9761169 www.chromtech.net.au 



Restek Product Instruction Sheet -- Rtx-5 MS 

Rtx®-5 MS 

GC/MS ANALYSIS INSTRUCTIONS [#40-004A - 1/95] 

The Rtx®-5 MS capillary column has been specifically developed to guarantee low column bleed on 

GC/MS systems, reproducible response factors and high inertness toward active semi-volatile pollutants. 

The Rtx®reg;-5 MS polymer is batch tested by mass spec to ensure low column bleed and minimize 

bleed interferences with high molecular weight sample components such as polynuclear aromatic 

hydrocarbons. Additionally, all Rtx®-5 MS columns are extensively tested with an environmental test 

mixture to ensure high column inertness and efficiency. The low bleed properties of the Rtx®-5 MS can 

be destroyed if the column is not installed correctly. The following operating hints will ensure that your 

column operates to its maximum capabilities. 

Perform air and water check before elevating column temperature. 

A common problem seen with GC/MS systems is high column bleed after installation. This bleed results 

from exposing/conditioning the column at high temperatures in the presence of an air leak. Always leak 

check and perform an air and water check on the column before elevating it in temperature. An air and 

water check is performed by scanning the masses 18 and 28 and comparing their relative response to 

mass 69 when the tuning valve is opened. Mass 18 and 28 should always be less than 1% of mass 69 

before elevating the column in temperature, otherwise a leak is present. Although some MS software 

programs incorporate automatic air and water checks, they usually reduce electron multiplier voltages 

and do not give accurate results. Figure 1 illustrates an air and water check performed on a leak-free 

system. 

Measuring Column Bleed Correctly 

A common problem that occurs is the impression that the column has high bleed. The real problem is 

how the column bleed is measured on the GC/MS system. Some GC/MS data systems use an autoscale 

feature which normalizes the intensity axis to the largest peak in the chromatogram. If there are no peaks 

present, the chromatogram is drawn with the baseline at full scale, giving the illusion that the column has 

high bleed. The most accurate way to measure column bleed is to inject a known concentration of a 

component and temperature program the column to the maximum operating temperature of the method. 

Measure the peak height of the component and compare it to the baseline offset from the bleed. Figure 2 

illustrates the effect of measuring column bleed with no peaks present in the chromatogram and by using 

a test mixture of known concentration. As seen in the figure, without injecting a component of known 

concentration, the bleed level appears much higher than it actually is. 

Polymer batch testing by GC/MS ensures low column bleed 

High column bleed can lead to inaccurate quantitative results, confused spectral interpretation, decreased 

sensitivity, and in extreme cases mis-identification. Each new Rtx®-5 MS polymer lot is batch tested by 

GC/MS with a temperature programmed test mix and must meet demanding criteria for low bleed before 

the polymer is accepted. The attached GC/MS bleed chromatogram shows the low MS bleed 

representative of this polymer. 

http://www.restekcorp.com/rtx5ms_.htm (1 of 3) [8/18/01 6:53:02 PM]


Thorough QA testing of each Rtx®-5 MS guarantees consistent results 

All Rtx®-5 MS columns are evaluated with a specially designed, temperature programmed test mixture 

to meet strict performance requirements. This test mixture contains some of the most sensitive semivolatile 

compounds for testing inertness and is temperature programmed to monitor bleed at the column's 

maximum operating temperature. The attached chromatogram shows the environmental test mixture on 

the Rtx®-5 MS. Excellent response of active pollutants such as 4-nitrophenol, 2,4-dinitrophenol, and 

pentachlorophenol is achieved. Additionally, high column efficiency is demonstrated by the resolution of 

the PNA isomers, benzo(b) and (k) fluoranthene. 

Figure 1: An air and water check performed on a leak-free system. 

Figure 2: The autoscaling feature of some GC/MS systems often gives the impression of high column 

bleed unless a peak of known concentration is present. 



Contact Restek Technical Service (or one of our authorized distributors) if you have questions about the 

Rtx®-5 MS column or any other Restek product. 




Restek Product Instruction Sheet -- Silcosteel® Packed Columns 

Silcosteel® Packed Columns 

[#81-008A - Rev. 2/96] 

OVERVIEW 

All Restek packed columns are preconditioned requiring only a brief 30 minute stabilization time. They 

are packed with a 2 inch void on the inlet end to facilitate on-column injection. Operating temperature 

limits for most phases available from Restek are listed in Table 1. Adaptors are usually required for 

installation into 1/4" injection packed ports since Silcosteel® columns have smaller outside diameters 

than corresponding glass packed columns with the same inside diameter. 


On-Column Injection 

1. Connect column to the injector by using either an instrument manufacturers inlet adaptor or the 

adaptor supplied by Restek. 

❍ 

For 2mm ID Silcosteel packed columns use Inlet Adaptor (cat.# 21651) with a 1/4"x1/8" 

Vespel®/graphite reducing ferrule. 

❍ For 4mm ID Silcosteel® packed columns use Restek's On-column Adaptor (cat.# 21650) 

with a 1/4" x 3/16" Vespel®/graphite reducing ferrule. 

2. Connect column to the detector using either the instrument manufacturer's adaptor or with the 1/4" 

reducing Vespel®/graphite reducing ferrule supplied. 

3. Turn the carrier gas on and set the flow rate to the desired level. Purge the column with carrier gas 

at ambient temperature for 10 minutes to remove any trapped air. 

4. Condition the column briefly for 30 minutes at the maximum temperature required for your 

application. Do not exceed the temperature required for your application. Do not exceed the 

temperature limit of packing listed in Table 1. 

HELPFUL HINTS 

Bending Tubing: Columns may be bent to facilitate installation in various instruments. Avoid kinking or 

bending the tubing in sharp 90 degree angles to prevent damage to the Silcosteel® layer. 

Handling CarboBlack Columns: Avoid rough handling or sharp bends to prevent damage to fragile 

CarboBlack supports (B & C). CarboBlack columns can be easily overloaded. Keep neat sample 

injections volumes below 0.2µl. If diluted samples are injected, try not to exceed 70µg on-column for 

CarboBlack B and 35µg for CarboBlack C packings. 

http://www.restekcorp.com/s_spakcl.htm (1 of 2) [8/18/01 6:55:35 PM]

Restek Product Instruction Sheet -- Silcosteel® Packed Columns 

Detector and Injector Temperatures: Be certain that heated zones do not exceed the upper temperature 

limit of the packing material listed in Table 1. 

Column Operating Pressure: Table 2 lists some of the common pressure drops for 2 meter x 2mm ID 

packed columns with different supports. 

Column Removal or Changing Septa: 

1. Lower heated zones to ambient temperature. 

2. Turn off carrier gas flow and allow sufficient time for the inlet pressure to reach ambient pressure. 

(Removing the column or changing septa will disturb the packed bed if the column inlet is rapidly 

depressurized). 

Table 1 || Temperature Constraints 

Table 2 || Pressure Drop 

Contact Restek Technical Service (or an authorized distributor) if you have questions about Silcosteel® 

Packed Columns or any other Restek product. 



http://www.restekcorp.com/s_spakcl.htm (2 of 2) [8/18/01 6:55:35 PM]

Restek Product Instruction Sheet -- Rt-QPLOT Capillary Column 

Rt-QPLOT Capillary Column 

[#40-005A - 4/95] 

HANDLING HINTS 

1. Temperature limitations: 

The Rt-QPLOT is stable to 250°C. 

2. Installation: 

Install the column into the injection port as directed in the column installation guide provided. 

Before installing the column into the detector, establish the desired carrier flow. 

3. Water Contamination: 

The Rt-QPLOT column will adsorb water which may affect subsequent analyses. By 

conditioning the Rt-QPLOT at 225°C for four hours, the water can be desorbed and the original 

selectivity returned. 

4. Conditioning: 

The Rt-QPLOT column should be conditioned for at least 4 hours prior to use. The 

conditioning temperature should be at least 20°C higher than the desired use temperature but 

should not exceed 250°C. Use at 250°C may require longer conditioning times to minimize 

column bleed. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Rt- 

QPLOT Column or any other Restek product. 



http://www.restekcorp.com/rtqplot_.htm [8/18/01 6:55:52 PM]

Restek Product Instruction Sheet -- Integra-Guard 

Integra-Guard Capillary Column 

[#40-007A - 10/95] 

The capillary column you have received has a “built-in” guard column. The built-in guard column is a 5- 

to 10-meter section of the capillary column that has been deactivated, but not coated with stationary 

phase. Since it is an integral part of the capillary column it has been tested for bleed and inertness along 

with the coated section of the column. 

The guard column should be installed into the 

inlet side of your GC system. When first 

received, you can easily identify the guard 

column end of the capillary column because it 

is marked with a tag labeled “Guard Column 

End”. Once this tag has been removed, you can 

still identify the guard column end since it is 

tied separately from the coated portion of the 

column on the side of the cage that the metal 

tag has been attached. 

Both the guard column and coated column 

sections are tied into approximately 5-meter 

bundles. You can determine the exact length of 

the guard column by counting the number of 

column loops and multiplying by 0.56 meters. 

As sections of the guard column become 

contaminated, they can be removed. Typically 1 or 2 loops from the inlet side will need to be removed to 

bring the column back to acceptable performance. Eventually, all of the guard column may be removed. 

Once this occurs, a new guard column can be installed using a Press-Tight® connector (cat.# 20400) or a 

Vu-Union® connector (cat.# 20418). For more information on guard columns and Press-Tight® or Vu- 

Union® connectors, please refer to Restek's Chromatography Products Catalog, or call Technical Service 

at 800-356-1688, ext. 4. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about the Integra- 

Guard or any other Restek product. 

http://www.restekcorp.com/integ_.htm (1 of 2) [8/18/01 6:56:35 PM]

Restek Product Instruction Sheet -- Integra-Guard 



http://www.restekcorp.com/integ_.htm (2 of 2) [8/18/01 6:56:35 PM]

Restek -- Packed Column Mesh Size Conversions 

Mesh Size Conversions 

Mesh 

20 

25 

30 

35 

40 

45 

50 

60 

70 

mm Microns 

0.84 

0.71 

0.59 

0.50 

0.42 

0.35 

0.30 

0.25 

0.21 

840 

710 

590 

500 

420 

350 

300 

250 

210 

Mesh 

80 

100 

120 

140 

170 

200 

230 

270 

mm Microns 

0.177 

0.149 

0.125 

0.105 

0.088 

0.074 

0.062 

0.053 

177 

149 

125 

105 

88 

74 

62 

53 

Packed Columns | Instruction Sheets 



http://www.restekcorp.com/meshes.htm [8/18/01 6:56:48 PM]

Restek Product Instruction Sheet -- FAMEWAX Capillary Column 

Analysis of Fatty Acid Methyl Esters from 

Menhaden Oil 

on the 30m X 0.25mm X 0.25µm FAMEWAX COLUMN 

[#40-009A - 11/95] 

cat.# 12497, lot# 694 

PEAK # FAME RETENTION TIME ECLs* 

RESOLUTION 

(R 1.5 is baseline sep) 

1 C14:0 6.741 14.00 

2 C16:0 9.330 16.00 

3 C16:1n7 9.659 16.29 

4 C16:2n4 10.372 16.88 

5 C16:3n4 10.784 17.20 

6 C16:4n1 11.487 17.72 

7 C18:0 11.882 18.00 

8 C18:1n9 12.134 18.22 

9 C18:1n7 12.212 18.28 

10 C18:1n5 12.366 18.41 

11 C18:2n6 12.670 18.67 

12 C18:2n4 12.924 18.87 

13 C18:3n6 13.034 18.96 

14 C18:3n4 13.279 19.15 

15 C18:3n3 13.452 19.29 

16 C18:4n3 13.821 19.57 

17 C18:4n1 13.958 19.67 

18 C20:0 14.413 20.00 

19 C20:1n9 14.551 20.11 

20 C20:1n7 14.652 20.19 

21 C20:2n6 15.138 20.56 

22 C20:3n6 15.497 20.83 

23 C20:4n6 15.818 21.06 

24 C20:3n3 16.053 21.23 

25 C20:4n3 16.472 21.52 

26 C20:5n3 16.865 21.79 

27 C22:0 17.179 22.00 

28 C22:1n9 17.541 22.19 

29 C22:2n6 18.338 22.58 

30 C21:5n3 18.884 22.85 

31 C23:0 19.213 23.00 2.57 (pks 30 & 31) 

32 C22:4n6 19.581 23.15 2.79 (pks 31 & 32) 

http://www.restekcorp.com/menhadn_.htm (1 of 3) [8/18/01 6:57:22 PM]


33 C22:5n6 20.266 23.44 

34 C22:5n3 21.263 23.83 

35 C24:0 21.719 24.00 

36 C22:6n3 22.090 24.14 2.63 (pks 35 & 36) 

37 C24:1n9 22.329 24.23 1.58 (pks 36 & 37) 

(conditions below) 



GC CONDITIONS: 

Oven temperature program: 130°C 6° min to 225°C (10 min.) 

Injector tempearture: 220°C and detector temperature: 230°C 

Carrier and linear velocity: H 2 set to 45cm/sec @ 130°C ( 1.121 min. dead time) 

Split vent flow: 40ml/min and total on-column concentration: 100-150 ng 


FAMEWAX column or any other Restek product. 






Menhaden Oil 


[#40-010A - 11/95] 

cat.# 12498, lot# 694 


RESOLUTION 


1 C14:0 6.672 14.00 

2 C16:0 9.485 16.00 

3 C16:1n7 9.852 16.29 

4 C16:2n4 10.633 16.86 

5 C16:3n4 11.094 17.18 

6 C16:4n1 11.880 17.70 

7 C18:0 12.362 18.00 

8 C18:1n9 12.658 18.23 

9 C18:1n7 12.746 18.30 

10 C18:1n5 12.908 18.42 

11 C18:2n6 13.252 18.68 

12 C18:2n4 13.540 18.89 

13 C18:3n6 13.661 18.98 

14 C18:3n4 13.942 19.18 

15 C18:3n3 14.142 19.32 

16 C18:4n3 14.564 19.60 

17 C18:4n1 14.717 19.70 

18 C20:0 15.168 20.00 

19 C20:1n9 15.428 20.19 

20 C20:1n7 15.538 20.27 

21 C20:2n6 16.049 20.63 

22 C20:3n6 16.414 20.88 

23 C20:4n6 16.740 21.10 

24 C20:3n3 16.983 21.26 

25 C20:4n3 17.410 21.53 

26 C20:5n3 17.820 21.79 

27 C22:0 18.158 22.00 

28 C22:1n9 18.515 22.18 

29 C22:2n6 19.275 22.56 

30 C21:5n3 19.829 22.82 

31 C23:0 20.210 23.00 2.52 (pks 30 & 31) 

http://www.restekcorp.com/menhadn2.htm (1 of 3) [8/18/01 6:57:41 PM]


32 C22:4n6 20.532 23.14 2.11 (pks 31 & 32) 

33 C22:5n6 21.203 23.41 

34 C22:5n3 22.192 23.81 

35 C24:0 22.698 24.00 

36 C22:6n3 23.020 24.12 2.07 (pks 35 & 36) 

37 C24:1n9 23.287 24.22 1.66 (pks 36 & 37) 







Carrier and linear velocity: H 2 set to 45cm/sec @ 130°C ( 1.121 min. dead time) 

Split vent flow: 40ml/min and total on-column concentration: 100-150 ng 








Menhaden Oil 


[#40-011A - 11/95] 

cat.# 12499, lot# 694 


RESOLUTION 


1 C14:0 9.144 14.00 

2 C16:0 12.239 16.00 

3 C16:1n7 12.629 16.28 

4 C16:2n4 13.498 16.88 

5 C16:3n4 13.988 17.20 

6 C16:4n1 14.820 17.72 

7 C18:0 15.281 18.00 

8 C18:1n9 15.571 18.21 

9 C18:1n7 15.671 18.28 

10 C18:1n5 15.859 18.41 

11 C18:2n6 16.224 18.65 

12 C18:2n4 16.524 18.86 

13 C18:3n6 16.659 18.94 

14 C18:3n4 16.952 19.14 

15 C18:3n3 17.163 19.27 

16 C18:4n3 17.641 19.57 

17 C18:4n1 17.827 19.69 

18 C20:0 18.347 20.00 

19 C20:1n9 18.691 20.17 

20 C20:1n7 18.843 20.25 

21 C20:2n6 19.223 20.44 

22 C20:3n6 20.122 20.86 

23 C20:4n6 20.614 21.09 

24 C20:3n3 20.969 21.25 

25 C20:4n3 21.610 21.53 

26 C20:5n3 22.190 21.78 

27 C22:0 22.728 22.00 

28 C22:1n9 23.285 22.19 

29 C22:2n6 24.480 22.58 

30 C21:5n3 25.338 22.85 

31 C23:0 25.844 23.00 1.55 (pks 30 & 31) 



32 C22:4n6 26.440 23.16 1.46 (pks 31 & 32) 

33 C22:5n6 27.519 23.44 

34 C22:5n3 29.068 23.82 

35 C24:0 29.834 24.00 

36 C22:6n3 30.353 24.12 1.39 (pks 35 & 36) 

37 C24:1n9 30.822 24.23 1.25 (pks 36 & 37) 





Carrier and linear velocity: H 2 set to 30cm/sec @ 120°C (1.667 min. dead time) 



Split vent flow: 40ml/min and total on-column concentration: 2,500 ng 






http://www.restekcorp.com/spetubes.htm 

Resprep SPE Tubes 

[#67-008A - 5/98] 

SPE SAMPLE 

PREPARATION STEPS: 

1. Conditioning 

● All SPE tubes should be conditioned to wet and settle the bed, remove 

any process contaminants, and activate the packing materials. 

● Use 1 to 2 column volumes of each solution (see table below). 

● Rinse tubes quickly. 

● Packing bed should remain wet before adding sample. 

2. Sample Addition 

● Adjust pH and dilute sample if necessary, before adding to tube. 

● Pass sample slowly (~5mL/min) through tube, or rapidly dropwise. 

● Match sample volume to tube/packed bed weight size. 

● Match analyte concentration to bed weight or exchange capacity of tube 

packing. 

● Pass sample completely through tube. 

http://www.restekcorp.com/spetubes.htm (1 of 5) [8/18/01 6:58:06 PM]


3. Washing 

● Use weak, dilute solutions and limit the amount to a maximum of 1 to 2 

column volumes. 

● Pass solutions quickly and completely through tube. 

4. Packing Drying(optional, when necessary) 

● Use analytical-grade, inert gases such as nitrogen, or draw vacuum 

through the tubes until the packing appears dry. 

● Use caution when drying semi-volatiles; always dry the minimum 

amount of time required (i.e. 1-10 minutes). 



5. Elution 

● Pass strong solvent or buffer solutions (see table below) through tube at a 

slow, dropwise rate. 

● Allow soak time on the packing to reach maximum extraction efficiency. 

● Obtain a more complete elution with multiple small volumes extracted 

entirely. 

● Total amount of extraction solvent should not exceed 1 to 5 column 

volumes. 

Tubes may be processed by any one or all of the 

techniques shown. 



Conditions for SPE Methods Development 

METHOD PACKINGS POLARITY 

Reversed 

Phase 

C18, C8, C2, 

Cyclohexyl, 

Cyano 

Normal Phase Silica, Florisil, 

Alumina, 

Cyano, Amino 

Ion Exchange See below 

Anion 

Exchange 

Cation 

Exchange 

SAX, NH2, 

Alumina 

SCX, 

Carboxylic 

acid, Alumina 

packing & 

analyte are 

nonpolar 

packing & 

analyte are 

polar 

packing & 

analyte carry an 

opposite 

electronic 

charge 

positively 

charged 

negatively 

charged 

SAMPLE 

MATRIX 

polar, often 

aqueous 

nonpolar, 

often an 

organic 

solvent 

aqueous 

or 

organic 

acids or 

negativelycharged 

analytes 

bases or 

positivelycharged 

analytes 

CONDITIONING 

SOLUTION 

a) methanol, then water 

or buffer (same as 

sample); 

b) final extraction 

solvent, then water 

miscible solvent, 

followed by water or 

buffer 

Fresh solvent (same as 

sample) 

methanol and weak 

buffer solution, pH 

determined by the 

phase type 

adjust pH 1-2 units 

above pKa of analyte 

adjust pH 1-2 units 

below pKa of analyte 

ELUTION 

SOLUTION 

nonpolar 

solvent or 

mixed solvent 

solutions 

additional clean 

solvent 

a) methanol 

only; 

b) pH adjusted 

methanol; 

c) pH adjusted 

water containing a 

strong counterion 

or a strong buffer 

solution. Bonded 

phase type will 

determine pH 

adjustment as 

needed. 

adjust pH 1-2 

units below 

pKa of analyte 

adjust pH 1-2 

units above pKa 

of analyte 

Contact Restek Technical Service (or an authorized distributor) if you have questions about Resprep 

SPE Tubes or any other Restek product. 






Restek Product Instruction Sheet -- 209 Chlorobiphenyl Congeners (PCBs) 

209 Chlorobiphenyl Congeners (PCBs) 

[#66-008A - Date 11/94] 

Version 1.0 - cat.# 21454 

PCBs are numbered consecutively from 1 to 209 as suggested by Ballschmitter and Zell. 

C13 

C14 

C15 

C16 

C17 

C18 

C19 

C20 

C21 

C22 

C23 

C24 

C25 

C28 

C32 

Biphenyl 

30. 

31. 

32. 

33. 

34. 

35. 

36. 

37. 

38. 

39. 

40. 

41. 

42. 

43. 

44. 

45. 

46. 

47. 

48. 

49. 

50. 

51. 

52. 

53. 

54. 

55. 

56. 

57. 

58. 

59. 

60. 

61. 

62. 

63. 

64. 

65. 

66. 

67. 

68. 

69. 

70. 

71. 

72. 

73. 

2,4,6 

2,4',5 

2,4',6 

2',3,4 

2',3,5 

3,3',4 

3,3',5 

3,4,4' 

3,4,5 

3,4',5 

2,2',3,3' 

2,2',3,4 

2,2',3,4' 

2,2',3,5 

2,2',3,5' 

2,2',3,6 

2,2',3,6' 

2,2',4,4' 

2,2',4,5 

2,2',4,5' 

2,2',4,6 

2,2',4,6' 

2,2',5,5' 

2,2',5,6' 

2,2',6,6' 

2,3,3',4 

2,3,3',4' 

2,3,3',5 

2,3,3',5' 

2,3,3',6 

2,3,4,4' 

2,3,4,5 

2,3,4,6 

2,3,4',5 

2,3,4',6 

2,3,5,6 

2,3',4,4' 

2,3',4,5 

2,3',4,5' 

2,3',4,6 

2,3',4',5 

2,3',4',6 

2,3',5,5' 

2,3',5',6 

75. 

76. 

77. 

78. 

79. 

80. 

81. 

82. 

83. 

84. 

85. 

86. 

87. 

88. 

89. 

90. 

91. 

92. 

93. 

94. 

95. 

96. 

97. 

98. 

99. 

100. 

101. 

102. 

103. 

104. 

105. 

106. 

107. 

108. 

109. 

110. 

111. 

112. 

113. 

114. 

115. 

116. 

117. 

118. 

2,4,4',6 

2',3,4,5 

3,3',4,4' 

3,3',4,5 

3,3',4,5' 

3,3',5,5' 

3,4,4',5 

2,2',3,3',4 

2,2',3,3',5 

2,2',3,3',6 

2,2',3,4,4' 

2,2',3,4,5 

2,2',3,4,5' 

2,2',3,4,6 

2,2',3,4,6' 

2,2',3,4',5 

2,2',3,4',6 

2,2',3,5,5' 

2,2',3,5,6 

2,2',3,5,6' 

2,2',3,5',6 

2,2',3,6,6' 

2,2',3',4,5 

2,2',3',4,6 

2,2',4,4',5 

2,2',4,4',6 

2,2',4,5,5' 

2,2',4,5,6' 

2,2',4,5',6 

2,2',4,6,6' 

2,3,3',4,4' 

2,3,3',4,5 

2,3,3',4',5 

2,3,3',4,5' 

2,3,3',4,6 

2,3,3',4',6 

2,3,3',5,5' 

2,3,3',5,6 

2,3,3',5',6 

2,3,4,4',5 

2,3,4,4',6 

2,3,4,5,6 

2,3,4',5,6 

2,3',4,4',5 

120. 

121. 

122. 

123. 

124. 

125. 

126. 

127. 

128. 

129. 

130. 

131. 

132. 

133. 

134. 

135. 

136. 

137. 

138. 

139. 

140. 

141. 

142. 

143. 

144. 

145. 

146. 

147. 

148. 

149. 

150. 

151. 

152. 

153. 

154. 

155. 

156. 

157. 

158. 

159. 

160. 

161. 

162. 

163. 

2,3',4,5,5' 

2,3',4,5',6 

2',3,3',4,5 

2',3,4,4',5 

2',3,4,5,5' 

2',3,4,5,6' 

3,3',4,4',5 

3,3',4,5,5' 

2,2',3,3',4,4' 

2,2',3,3',4,5 

2,2',3,3',4,5' 

2,2',3,3',4,6 

2,2',3,3',4,6' 

2,2',3,3',5,5' 

2,2',3,3',5,6 

2,2',3,3',5,6' 

2,2',3,3',6,6' 

2,2',3,4,4',5 

2,2',3,4,4',5' 

2,2',3,4,4',6 

2,2',3,4,4',6' 

2,2',3,4,5,5' 

2,2',3,4,5,6 

2,2',3,4,5,6' 

2,2',3,4,5',6 

2,2',3,4,6,6' 

2,2',3,4',5,5' 

2,2',3,4',5,6 

2,2',3,4',5,6' 

2,2',3,4',5',6 

2,2',3,4',6,6' 

2,2',3,5,5',6 

2,2',3,5,6,6' 

2,2',4,4',5,5' 

2,2',4,4',5,6' 

2,2',4,4',6,6' 

2,3,3',4,4',5 

2,3,3',4,4',5' 

2,3,3',4,4',6 

2,3,3',4,5,5' 

2,3,3',4,5,6 

2,3,3',4,5',6 

2,3,3',4',5,5' 

2,3,3',4',5,6 

165. 

166. 

167. 

168. 

169. 

170. 

171. 

172. 

173. 

174. 

175. 

176. 

177. 

178. 

179. 

180. 

181. 

182. 

183. 

184. 

185. 

186. 

187. 

188. 

189. 

190. 

191. 

192. 

193. 

194. 

195. 

196. 

197. 

198. 

199. 

200. 

201. 

202. 

203. 

204. 

205. 

206. 

207. 

208. 

2,3,3',5,5',6 

2,3,4,4',5,6 

2,3',4,4',5,5' 

2,3',4,4',5',6 

3,3',4,4',5,5' 

2,2',3,3',4,4',5 

2,2',3,3',4,4',6 

2,2',3,3',4,5,5' 

2,2',3,3',4,5,6 

2,2',3,3',4,5,6' 

2,2',3,3',4,5',6 

2,2',3,3',4,6,6' 

2,2',3,3',4',5,6 

2,2',3,3',5,5',6 

2,2',3,3',5,6,6' 

2,2',3,4,4',5,5' 

2,2',3,4,4',5,6 

2,2',3,4,4',5,6' 

2,2',3,4,4',5',6 

2,2',3,4,4',6,6' 

2,2',3,4,5,5',6 

2,2',3,4,5,6,6' 

2,2',3,4',5,5',6 

2,2',3,4',5,6,6' 

2,3,3',4,4',5,5' 

2,3,3',4,4',5,6 

2,3,3',4,4',5',6 

2,3,3',4,5,5',6 

2,3,3',4',5,5',6 

2,2',3,3',4,4',5,5' 

2,2',3,3',4,4',5,6 

2,2',3,3',4,4',5',6 

2,2',3,3',4,4',6,6' 

2,2',3,3',4,5,5',6 

2,2',3,3',4,5,6,6' 

2,2',3,3',4,5',6,6' 

2,2',3,3',4,5,5',6' 

2,2',3,3',5,5',6,6' 

2,2',3,4,4',5,5',6 

2,2',3,4,4',5,6,6' 

2,3,3',4,4',5,5',6 

2,2',3,3',4,4',5,5',6 

2,2',3,3',4,4',5,6,6' 

2,2',3,3',4,5,5',6,6' 

1. 

2. 

3. 

4. 

5. 

6. 

7. 

8. 

9. 

10. 

11. 

12. 

13. 

14. 

15. 

16. 

17. 

18. 

19. 

20. 

21. 

22. 

23. 

24. 

25. 

26. 

27. 

28. 

2 

3 

4 

2,2' 

2,3 

2,3' 

2,4 

2,4' 

2,5 

2,6 

3,3' 

3,4 

3,4' 

3,5 

4,4' 

2,2',3 

2,2',4 

2,2',5 

2,2',6 

2,3,3' 

2,3,4 

2,3,4' 

2,3,5 

2,3,6 

2,3',4 

2,3',5 

2,3',6 

2,4,4' 

http://www.restekcorp.com/pcb_209.htm (1 of 2) [8/18/01 6:58:28 PM]

Restek Product Instruction Sheet -- 209 Chlorobiphenyl Congeners (PCBs) 

29. 2,4,5 

74. 2,4,4',5 119. 2,3',4,4',6 164. 2,3,3',4',5',6 209. Decachlorobiphenyl 

Operating Conditions: 

Column: 60m, 0.25mm ID, 0.25µm Rtx®-5 (cat.# 10226) 

Carrier gas: hydrogen 

Dead time: 1.7 min. @ 100°C 

Slow Run: 100°C to 310°C @ 2°C/min. 

Fast Run: 100°C to 320°C @ 8°C/min. 

References used for congener identifications from standards and for Aroclor®: 

Ballschmitter, K. and Zell, M. Fresenius Z. Analytical Chemistry, 1980, 302, 20-31. 

Mullin, M.D.; Pochini, C.M.; McCrindle, S.; Romkes, M.; Safe, S.&L.M.; Env. Sci. Tech., 1984, 18, 468-476. 

Mullin, M.D., PCB Workshop, U.S. EPA Large Lakes Research Station, Grosselle, MI, June 1985. 

EPA Test Methods for Evaluating Solid Waste, SW-846. 

Contact Restek Technical Service (or an authorized distributor) if you have questions about this or any other Restek 

product. 


http://www.restekcorp.com/pcb_209.htm (2 of 2) [8/18/01 6:58:28 PM]

Restek Corporation -- Site Index 

Index 

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Restek technical literature for which you're looking. 

Chromatogram Search Wizard 

Search for chromatograms by peak, phase, and keyword. Find 

what you need in our extensive archive of GC and HPLC 

applications. 

RESTEK CORP (US) Toll-free (800) 356-1688 

110 Benner Circle, Bellefonte, PA 16823-8812 Phone: (814) 353-1300 FAX (814) 353-1309 

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Schaberweg 23 

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Phone: 49-06172-2797-0 

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restekfr@club-internet.fr 

1, rue Montespan 

91024 Evry, France 

Phone: 33-01-60-78-32-10 

Fax: 33-01-60-78-70-90 

Thames Restek UK LTD 

Sales@Thamesrestek.co.uk 

Fairacres Industrial Centre 

Dedworth Road, Windsor, England 

Berkshire SL4 4LE 

Phone: 44-1753-624111 

Fax: 44-1753-624666 

New! Products Service Library Jobs Index Home Ordering 


http://www.restekcorp.com/index.htm [8/18/01 7:08:44 PM]

Restek -- Product Instruction Sheets - Chromtech.com.au

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