Tilletia indica - The Food and Environment Research Agency - Defra
Tilletia indica - The Food and Environment Research Agency - Defra
Tilletia indica - The Food and Environment Research Agency - Defra
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Sample<br />
declared<br />
healthy<br />
IDENTIFICATION<br />
based on teliospore<br />
morphology<br />
(Protocol B)<br />
Wash test for tuberculate <strong>Tilletia</strong> teliospores<br />
(Size selective sieving on 50 g sub-samples)<br />
(Protocol A)<br />
negative positive<br />
Many teliospores<br />
present<br />
(c. ≥10)<br />
MORPHOLOGICAL<br />
DIAGNOSIS of<br />
teliospores<br />
(Protocol B)<br />
MOLECULAR CONFIRMATION TESTS<br />
Isolate & germinate suspect teliospores to<br />
produce cultures for molecular confirmation tests<br />
(Protocol C)<br />
MOLECULAR CONFIRMATION:<br />
Restriction enzyme analysis (Protocol D)<br />
PCR with species specific primers (Protocol E)<br />
TaqMan with species specific primers & probe (Protocol F)<br />
IDENTIFICATION OF SPECIES<br />
based on morphological <strong>and</strong> molecular analyses<br />
CONFIRMATION:<br />
microscopic examination of<br />
teliospores from bunted<br />
seeds compared to those in<br />
wash test (Protocol B)<br />
Bunted seeds found<br />
Examine sample for bunted<br />
cereal seeds or bunted seeds<br />
of other Poaceae<br />
No bunted cereal grains<br />
found<br />
Few teliospores present<br />
(c.