Tilletia indica - The Food and Environment Research Agency - Defra

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D. Protocol for confirmation by traditional PCR using species specific primers The draft protocol is currently being evaluated by the ring testing participants. The protocol will be finalised when comments have been received. E. Protocol for confirmation by PCR using TaqMan® The draft protocol is currently being evaluated by the ring testing participants. The protocol will be finalised when comments have been received. F. Protocol for confirmation using restriction enzyme analysis The draft protocol is currently being evaluated by the ring testing participants. The protocol will be finalised when comments have been received. 33
SECTION IV: REFERENCES Aggarwal, R, Joshi, LM & Singh, DV (1990). Morphological differences between teliospores of Neovossia indica and N. horrida. Indian Phytopathology, 43, 439-442. Aggarwal, VK & Mathur, SB. (1992). Detection of Karnal bunt in wheat seed samples treated with fungicides. FAO Plant Protection Bulletin, 40, 148-153. Bansal, R, Singh, DV & Joshi, LM (1984). Comparative morphological studies in teliospores of Neovossia indica. Indian Phytopathology, 37, 355-357. Bonde, MR, Nester, SE, Smilanick, JL, Frederick, RD & Schaad, NW (1999). Comparison of effects of acidic electrolysed water and NaOCl on Tilletia indica teliospore germination. Plant disease, 83, 627-632. CABI/EPPO (1997). Quarantine Pests for Europe (second edition). CAB International, Cambridge University Press. Castlebury, LA (1998). Morphological characterisation of Tilltia indica and similar fungi. Pages 97-105 in: Bunts and Smuts of Wheat: An International Symposium. V.S. Malik and D.E. Mathur, eds. North American Plant Protection Organisation, Ottowa. Castlebury, LA & Carris, LM (1999). Tilletia walkeri, a new species on Lolium multiflorum and L. perenne. Mycologia 91, 121-131. CMI (1983). Description of Pathogenic Fungi and Bacteria, No. 748, Tilletia indica. CAB International, Wallingford, UK. CMI (1965). Description of Pathogenic Fungi and Bacteria, No. 75, Tilletia barclayana. CAB International, Wallingford, UK. Cunfer, BM & Castlebury, LA (1999). Tilletia walkeri on annual ryegrass in wheat fields in the southeastern United States. Plant Disease 83, 685-689. Durán, R & Fischer, GW (1961). The Genus Tilletia. Washington State University. Durán, R (1987). Usilaginales of Mexico: Taxonony, symptomatology, spore germination and basidial cytology. Washington State University. Frederick, RD, Snyder, KE, Tooley, PW, Berthier-Schaad, Y, Peterson, GL, Bonde, MR, Schaad, NW & Knorr, DA (2000). Identification and differentiation of Tilletia indica and T. walkeri using the polymerase chain reaction. Phytopathology, 90, 951-960. Hawksworth, DL, Kirk, PM, Sutton, BC & Pegler, DN (1995). Ainsworth & Bisby’s Dictionary of the Fungi (Eighth Edition). CAB International, Wallingford, UK. Khanna, A & Payak, MM (1968). Teliospore morphology of some smut fungi. II. Light microscopy. Mycologia, 60, 655-662. 34
Page 1 and 2: EU RECOMMENDED PROTOCOL FOR THE DIA
Page 3 and 4: SECTION IV: REFERENCES ** 3
Page 5 and 6: LIST OF FIGURES Figure 1.1. Grain i
Page 7 and 8: SECTION II: DIAGNOSTIC SCHEME Scope
Page 9 and 10: Sampling Seed lots should be sample
Page 11 and 12: median profiles can be enhanced by
Page 13 and 14: Figure 2.2. Teliospores of Tilletia
Page 15 and 16: Figure 2.4. Teliospores of Tilletia
Page 17 and 18: 7 days 10 days 14 days T. horrida T
Page 19 and 20: conform to one specific species (si
Page 21 and 22: SECTION III. PROTOCOLS A1. Protocol
Page 23 and 24: 25. Finally, bleach all equipment u
Page 25 and 26: Figure 3.6. Tilletia indica teliosp
Page 27 and 28: A2. Protocol for extracting teliosp
Page 29 and 30: Teliospore Size (µm) Colour Orname
Page 31 and 32: Sample d (✔ boxes) T. horrida T.
Page 33 and 34: C. Protocol for isolation and germi
Page 35: Materials Aspirator bottle with dis

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