D. Protocol for confirmation by traditional PCR using species specific primers <strong>The</strong> draft protocol is currently being evaluated by the ring testing participants. <strong>The</strong> protocol will be finalised when comments have been received. E. Protocol for confirmation by PCR using TaqMan® <strong>The</strong> draft protocol is currently being evaluated by the ring testing participants. <strong>The</strong> protocol will be finalised when comments have been received. F. Protocol for confirmation using restriction enzyme analysis <strong>The</strong> draft protocol is currently being evaluated by the ring testing participants. <strong>The</strong> protocol will be finalised when comments have been received. 33
SECTION IV: REFERENCES Aggarwal, R, Joshi, LM & Singh, DV (1990). Morphological differences between teliospores of Neovossia <strong>indica</strong> <strong>and</strong> N. horrida. Indian Phytopathology, 43, 439-442. Aggarwal, VK & Mathur, SB. (1992). Detection of Karnal bunt in wheat seed samples treated with fungicides. FAO Plant Protection Bulletin, 40, 148-153. Bansal, R, Singh, DV & Joshi, LM (1984). Comparative morphological studies in teliospores of Neovossia <strong>indica</strong>. Indian Phytopathology, 37, 355-357. Bonde, MR, Nester, SE, Smilanick, JL, Frederick, RD & Schaad, NW (1999). Comparison of effects of acidic electrolysed water <strong>and</strong> NaOCl on <strong>Tilletia</strong> <strong>indica</strong> teliospore germination. Plant disease, 83, 627-632. CABI/EPPO (1997). Quarantine Pests for Europe (second edition). CAB International, Cambridge University Press. Castlebury, LA (1998). Morphological characterisation of Tilltia <strong>indica</strong> <strong>and</strong> similar fungi. Pages 97-105 in: Bunts <strong>and</strong> Smuts of Wheat: An International Symposium. V.S. Malik <strong>and</strong> D.E. Mathur, eds. North American Plant Protection Organisation, Ottowa. Castlebury, LA & Carris, LM (1999). <strong>Tilletia</strong> walkeri, a new species on Lolium multiflorum <strong>and</strong> L. perenne. Mycologia 91, 121-131. CMI (1983). Description of Pathogenic Fungi <strong>and</strong> Bacteria, No. 748, <strong>Tilletia</strong> <strong>indica</strong>. CAB International, Wallingford, UK. CMI (1965). Description of Pathogenic Fungi <strong>and</strong> Bacteria, No. 75, <strong>Tilletia</strong> barclayana. CAB International, Wallingford, UK. Cunfer, BM & Castlebury, LA (1999). <strong>Tilletia</strong> walkeri on annual ryegrass in wheat fields in the southeastern United States. Plant Disease 83, 685-689. Durán, R & Fischer, GW (1961). <strong>The</strong> Genus <strong>Tilletia</strong>. Washington State University. Durán, R (1987). Usilaginales of Mexico: Taxonony, symptomatology, spore germination <strong>and</strong> basidial cytology. Washington State University. Frederick, RD, Snyder, KE, Tooley, PW, Berthier-Schaad, Y, Peterson, GL, Bonde, MR, Schaad, NW & Knorr, DA (2000). Identification <strong>and</strong> differentiation of <strong>Tilletia</strong> <strong>indica</strong> <strong>and</strong> T. walkeri using the polymerase chain reaction. Phytopathology, 90, 951-960. Hawksworth, DL, Kirk, PM, Sutton, BC & Pegler, DN (1995). Ainsworth & Bisby’s Dictionary of the Fungi (Eighth Edition). CAB International, Wallingford, UK. Khanna, A & Payak, MM (1968). Teliospore morphology of some smut fungi. II. Light microscopy. Mycologia, 60, 655-662. 34