Tilletia indica - The Food and Environment Research Agency - Defra
Tilletia indica - The Food and Environment Research Agency - Defra
Tilletia indica - The Food and Environment Research Agency - Defra
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Bunted wheat seeds found Bunted wheat NOT<br />
found; Bunted ryegrass<br />
found<br />
CONFIRMATION<br />
Karnal bunt (T. <strong>indica</strong>)<br />
confirmed by microscopic<br />
examination of teliospores<br />
from grains<br />
(Table 2.2 & 3.2)<br />
Molecular confirmation of<br />
teliospores in wash tests<br />
recommended in case of<br />
mixed species populations<br />
ALL morphological<br />
characters satisfy one<br />
specific <strong>Tilletia</strong> species<br />
(see Table 3.2)<br />
MORPHOLOGICAL<br />
IDENTIFICATION<br />
Tuberculate teliospores detected in wash test:<br />
record morphological characters (Table 3.1)<br />
Examine grain sample* <strong>and</strong> the washed sub-samples directly for<br />
bunted seeds of wheat or other Poaceae (e.g. ryegrass)<br />
CONFIRMATION<br />
Ryegrass bunt (T. walkeri)<br />
confirmed if teliospores<br />
from bunted seeds <strong>and</strong><br />
wash test are identical <strong>and</strong><br />
conform totally to T.<br />
walkeri (Table 2.2 & 3.2)<br />
Many teliospores present<br />
(≥10)<br />
NOT all morphological<br />
characters satisfy one<br />
<strong>Tilletia</strong> species<br />
(see Table 3.2)<br />
PRESUMPTIVE<br />
IDENTIFICATION<br />
Bunted seeds<br />
NOT found<br />
PRESUMPTIVE<br />
IDENTIFICATION<br />
Assess morphological<br />
characters of teliospores<br />
i n wash test (Table 3.1)<br />
Few teliospores present<br />
(