Tilletia indica - The Food and Environment Research Agency - Defra

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Tilletia indica Tilletia walkeri Tilletia horrida Densely echinulate (DE) Densely echinulate (DE) to finely cerebriform (FC: closely spaced, narrow ridges) Coarsely cerebriform (CO): Coarsely arranged spines forming wide incompletely cerebriform ridges Incompletely cerebriform to coralloid ridges (CC) Thick clumps (TC) Figure 3.7. Pictorial key to teliospore ornamentation (see also Figs 2.2–2.4). Spines as polygonal scales (PS), sometimes forming ridges or clumps (left and centre); Sharply pointed, often curved spines (right) 30
Sample d (✔ boxes) T. horrida T. walkeri T. indica Max Size (diam., µm) a Mean size (diam., µm) a 36–45–50+ 24–28 30–31 35–41 Pale yellow to mostly light or dark chestnut- brown (to semi-opaque) Colour b Spines (ornamentation) in surface view b and median profile b,c Pale yellow to mostly reddishbrown (never opaque) Pale orange, but mostly dark reddishbrown to opaque black Echinulate; polygonal scales in surface view; occasionally cerebriform ridges or rarely clumps. Sharpely pointed, becoming truncate, occasionally curved Coarse; broad, incompletely cerebriform ridges (to coralloid), or thick clumps. Conical to truncate (gaps between spines obvious in profile after bleaching) c Dense; echinulate or closely spaced narrow ridges (finely cerebriform) Sharpely pointed to truncate, occasionally curved (few or no gaps between spines after bleaching) c Table 3.2. Scheme for morphologically distinguishing teliospores of Tilletia indica, T. walkeri and T. horrida detected in size-selective sieving wash tests that use 20 µm and 53 µm sieves. a Section II, Table 2.2; b Section II, Table 2.2 and Figures 2.2–2.4; c Section II, Figure 2.5; d Refer to Diagnostic Scheme in Section II (Figure 2.1) and Decision scheme (Figure 3.8). , character conforms to species
Page 1 and 2: EU RECOMMENDED PROTOCOL FOR THE DIA
Page 3 and 4: SECTION IV: REFERENCES ** 3
Page 5 and 6: LIST OF FIGURES Figure 1.1. Grain i
Page 7 and 8: SECTION II: DIAGNOSTIC SCHEME Scope
Page 9 and 10: Sampling Seed lots should be sample
Page 11 and 12: median profiles can be enhanced by
Page 13 and 14: Figure 2.2. Teliospores of Tilletia
Page 15 and 16: Figure 2.4. Teliospores of Tilletia
Page 17 and 18: 7 days 10 days 14 days T. horrida T
Page 19 and 20: conform to one specific species (si
Page 21 and 22: SECTION III. PROTOCOLS A1. Protocol
Page 23 and 24: 25. Finally, bleach all equipment u
Page 25 and 26: Figure 3.6. Tilletia indica teliosp
Page 27 and 28: A2. Protocol for extracting teliosp
Page 29: Teliospore Size (µm) Colour Orname
Page 33 and 34: C. Protocol for isolation and germi
Page 35 and 36: Materials Aspirator bottle with dis
Page 37 and 38: SECTION IV: REFERENCES Aggarwal, R,

Tilletia indica - The Food and Environment Research Agency - Defra

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