Tilletia indica - The Food and Environment Research Agency - Defra

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Figure 3.3. Size-selective sieving wash test: grain and washings poured onto a 53 µm sieve over a 500 ml Erlenmeyer flask, together with an aspirator bottle for subsequent rinsing of the grain on the sieve. Figure 3.4. Size-selective sieving wash test: the final sieve fraction being washed to one side of the 20 µm sieve with water from a disposable Pasteur pipette in preparation for recovery. Figure 3.5. Size-selective sieving wash test: the final sieve fraction being recovered from the 20 µm sieve with water from a disposable Pasteur pipette for subsequent centrifugation in a conical centrifuge tube. 24
Figure 3.6. Tilletia indica teliospores in median view (20–50 µm diam.; mean 35–41 µm). 25
Page 1 and 2: EU RECOMMENDED PROTOCOL FOR THE DIA
Page 3 and 4: SECTION IV: REFERENCES ** 3
Page 5 and 6: LIST OF FIGURES Figure 1.1. Grain i
Page 7 and 8: SECTION II: DIAGNOSTIC SCHEME Scope
Page 9 and 10: Sampling Seed lots should be sample
Page 11 and 12: median profiles can be enhanced by
Page 13 and 14: Figure 2.2. Teliospores of Tilletia
Page 15 and 16: Figure 2.4. Teliospores of Tilletia
Page 17 and 18: 7 days 10 days 14 days T. horrida T
Page 19 and 20: conform to one specific species (si
Page 21 and 22: SECTION III. PROTOCOLS A1. Protocol
Page 23: 25. Finally, bleach all equipment u
Page 27 and 28: A2. Protocol for extracting teliosp
Page 29 and 30: Teliospore Size (µm) Colour Orname
Page 31 and 32: Sample d (✔ boxes) T. horrida T.
Page 33 and 34: C. Protocol for isolation and germi
Page 35 and 36: Materials Aspirator bottle with dis
Page 37 and 38: SECTION IV: REFERENCES Aggarwal, R,

Tilletia indica - The Food and Environment Research Agency - Defra

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