Pakistan Standard specification For Apple juice

PS: 1739 - 1985
APPENDIX C
Table 1 Item II
DETERMINATION OF VOLATILE OIL
C-1 Apparatus ― The apparatus (see Fig. I) consists essential of three
parts namely, (a) a round-bottom flask, in which is placed the
material containing the volatile oil and given quantity of water, (b) a
separator (see Fig. 2) in which the oil is automatically separated from
the distillate in a graduate tube, thereby permitting a direct reading of
the quantity of the oil and (c) a convenient condenser. The size of the
flask may vary from 1000ml to approximately two litres depending
upon the nature of the material and the percentage of volatile oil.
The flask is not heated by a direct flame on account of the danger of
charring the material containing the volatile oil. An oil-bath heated
electrically or by a suitable gas flame will be satisfactory.
C-2 Reagent
C-2.1
Wax-Carnauba
C-3 Procedure
C-3.1
Weigh out accurately a suitable quantity (100 to 150ml) if the well
mixed juice to a flask which may vary in capacity from 1000ml to 2
litres depending upon the nature of the material and the percentage
of volatile oil. The quantity of the material taken should be such as to
obtain, if possible, from 0.1 to 0.3ml of volatile oil. Add water to fill
the flask slightly loss than half full and mix by swirling. Add glass
beads and a piece of carnauba wax about 1.5mm in diameter. If
foaming persists cool immediately and add a few drops of a aqueous
solution of a wetting agent. Connect the flask through the calibrated
oil trap to the condenser. The trap and condenser shall be until there
is no increase in the oil content over a period of one hour, but in any
case, not less than for 4 hours.