spectrophotometric determination of naproxen and esomeprazole in ...

Neha A.Jain et al. / International Journal of Pharma Sciences and Research (IJPSR)
Vol.2(5), 2011,130-134
coefficient of 0.999 for EOZ respectively. The low % RSD values of 0.414 for NP and 0.412 for EOZ were
observed for analysis of 5 replicate samples, indicating precision and reproducibility.NP exhibits its maximum
absorption at 232nm and obeyed Beer’s law in the range of 1-5µg/ml and EOZ exhibits its maximum absorption
at 301.5 nm and obeyed Beer’s law in the range of 4-12µg/ml. The results of analysis and recovery studies are
presented in the Table. The percentage recovery value 98.23% and 98.87% for NP and EOZ respectively
indicates that there is no interference from the excipients present in laboratory mixture. The developed method
was found to be sensitive, accurate, precise and reproducible and can be applicable for the analysis of NP and
EOZ in laboratory mixtures.
TABLE: Analysis of dosages forms and Recovery studies.
Product Drug Labelclaim % Estimated * % RSD % Recovery† % RSD of recovery
Lab NP 20 mg 100.97 0.41 98.23 1.23
Mixture EOZ 80 mg 102.01 1.11 98.87 1.34
NP: Naproxen; EOZ: Esomeprazole; RSD: Relative standard deviation;
* Indicates mean of three determinations (n=3);
†Indicates mean of three recovery studies at 80%, 100% and 120% level.
Table: Beer’s Lambert study of lab mixture
Concentration Absorbance Area Under Curve
2 0.057 0.5591
4 0.093 0.8979
6 0.145 1.4266
8 0.197 1.7963
10 0.234 2.298
0.3
0.2
0.1
0
ABS
0 5 10 15
y = 0.0235x + 0.0037
R² = 0.9968
ABS
Linear
(ABS)
3
2
1
0
AUC
0 5 10 15
y = 0.2233x + 0.0232
R² = 0.9917
AUC
Linear
(AUC)
ACKNOWLEDGEMENTS:
The authors are grateful to Dr. Reddy’s Laboratory, for providing gift samples of drug for research work and
also thankful to Principal, S.K.B.College of Pharmacy, New Kamptee for providing laboratory facilities and
constant encouragement.
REFERENCES:
[1] United States Pharmacopoeia and National Formulary; (24th) Asian Edition, The United States Pharmacopoeia Convention Inc,
U.S.A, 2709-3259.
[2] Fun, LW. (2002). MIMS Bangladesh. Medimedia, Singapore. p. 53-67.
[3] Scott LJ , Dunn CJ , Mallarkey G , Sharpe M .Esomeprazole – A review of its use in the management of acid-related disorders.
Drugs 2002;62:1503-38.
[4] Sean C Sweetman. Martindale-The completer drug reference. 2002, 33 rd edition: 1225.
[5] Estimation of Esomeprazole and Domperidone by absorption ratio method in Pharmaceutical Dosage by Priti D Trivedi and Dilip G
Maheshwari * in International Journal of ChemTech Research in Vol.2, No.3 July-Sept 2010, pp-1598-1605.
ISSN : 0975-9492 133