FINAL REPORT

4. Determination of total content of P
Methodology
The method is based on phosphate ion’s property which can show a purple colour with
molybdate ion.And we can determine phophorus by measuring colour intensiveness.
Materials
• Ammonium chloride
• Ammonium molybdate
• Potassium permanganate
• Glucose
• Hydrazine sulfate
• Standard solution of phosphorus, ootassium dihydrophosphate
• Hot plate
Experimental procedure
Measure 10ml ammonium chloride extract and add 1ml potassium permanganate 0.4N and
ammonium molybdate. Boil it 2 minute, and add glucose until the solution has no colour.
Then add hydrazine sulfate 0.5ml and wait 10 –20 min. After that measure the intensive of
this solution by PEC –56. To calculate, need results of standard solution.
5. Determination of ammonium an nitrate nitrogen
Methodology
The method is based on ammonium ion’s property which can show a yellow color with
Nessler reagent. For the nitrate nitrogen, color reagent is sulfophenol. And we can determine
nitrogen by measuring color intensiveness.
Materials
• Standard solution, Ammonium chloride
• Standard solution, potassium nitrate
• Nessler reagent
• Sodium tartrat, Segnetov’s salt
• Sulfophenol
Experimental procedure
Measure 10ml water extract and add 1ml Segnetov’s salt. Add 1ml Nessler reagent and add
1ml Segnetov’s soil again. Wait until solution has a constant color. After that measure the
intensive of this solution by PEC –56. To calculate, need results of standard solution. For
63