graecum l. cell suspension culture. - international indexed refereed ...

International Referred Reseach Journal,February,2011 ISSN-0975-3486 RNI: RAJBIL 2009/300097 VOL-I *ISSUE 17
February, 2011
Research Paper—Environmental Science
EFFECT OF SALICYLIC ACID ON
TRIGONELLINE PRODUCTION IN TRIGONELLA
FOENUM- GRAECUM L. CELL
SUSPENSION CULTURE.
* Laxmi Mathur ** Rajesh Kr. Yadav
*-** Dept. of Environmental Science, S.S. Jain Subodh (PG) College, Jaipur
A B S T R A C T
Trigonelline (a pyridine alkaloid) was extracted from callus of Trigonella foenum-graecum. Cell suspension
culture of T. foenum-graecum was prepared to study the effect of elicitation. Two concentration doses of
salicylic acid (viz. 0.025 mM and 0.05 mM) were used as elicitor in the cell suspension culture. A two fold
increase in trigonelline production was observed at a concentration dose of 0.025 mM, while an increase
was also observed at 0.05mM concentration dose of salicylic acid.
Key words: Trigonelline, Trigonella foenum-graecum, Cell suspension culture, Elicitation, Salicylic acid.
Introduction:
Secondary metabolites are the compounds which play
an important role in plant defense mechanism.
Trigonelline, one of the pyridine alkaloid is widely
distributed in the plant Kingdom (Taguchi et. al., 1985).
Elicitation can enhance the efficiency of product
accumulation in cell cultures reducing the time required
to obtain a product (Wiznsama et. al., 1985).
Trigonella foenum-graecum L. commonly
known as fenugreek (Methis) is a rich source of
trigonelline. Enhancement in trigonelline content in
cell suspension culture of T. foenum-graecum using
salicylic acid as an elicitor showed a significant increase
at dose level of 0.025 mM and 0.05 mM concentrations
of salicylic acid as compared to normal callus.
Material and Methods:
Cell suspension cultures of T. foenum-graecum
were grown in liquid MS medium. Salicylic acid (Merck
India Ltd.) was used as an elicitor, two concentration
doses of salicylic acid (0.025 mM and 0.05 mm) were
added in both the sets of experiment, separately by
using erilson’s micropipette.
The flasks were incubated on shaker for 24
hours of incubation; callus tissue was weighed and
separated from the medium by filtration through
Whatmann filter paper. Both tissue and culture medium
were used for extraction and estimation of their
trigonelline content.
The dried callus samples and culture medium
were extracted and analysed according to method of
Kogan et. al. (1953). The extracted samples were
dissolved in 0.5 ml methanol and co-chromatographed
on tlc along with standard trigonelline.
Results:
The effect of low concentration doses (0.025
mM and 0.05 mM) of salicylic acid on the production
of trigonelline was observed with control. The callus
growth was not effected throughout the experiment,
while a very significant two fold increase (0.59%) in
trigonelline content was observed in 0.025 mM
treatment dose of salicylic acid over the control. The
trigonelline content was also increased (0.39%) in 0.05
mM (Table-1).
Table – 1
Effect of different dose treatment of Salicylic acid on
trigonelline content in T. foenum-graceum cell
suspension culture.
Control
Trigonelline
Salicylic acid dose
0.025 mM 0.05mM
(%) 0.27 0.59 0.39
Discussions:
Elicitor in cell cultures offer a novel approach
for rapid accumulation of certain secondary metabolites
by reducing time required to obtain the efficiency of
product (Kamal et. al. 1995). Rokem et. al. (1984) reported
70% increase in disogenin content from Dioscorea
deoltoidea cell cultures by using homogenate of
Rhizopous arrhizus.Salicylic alcohol and salicylic acid
are the basic compound of salicylate an important group
of pharmaceutical agents.
R E S E A R C H A N A L Y S I S A N D E V A L U A T I O N 137

International Referred Reseach Journal,February,2011 ISSN-0975-3486 RNI: RAJBIL 2009/300097 VOL-I *ISSUE 17
It has been observed that application of recovery in the tissue was highly significant in
exogenous salicylic acid induces expression of defense comparison to control.This increase in trigonelline
related genes and provides partial protection against content can also be co-related with the studies of Kamal
pathogen.Ward (1991) reported salicylic acid plays an et. al. (1995), that the salicylic acid might be acting as
important role in disease resistant in tobacco.In the a signal compound in the induction of plant defense
present study two months old callus culture of T. mechanism thereby increasing the production of
foenum-graecum were fed with different treatment secondary metabolites specifically trigonelline in the
doses of salicylic acid in the medium. Trigonelline present investigations on T. foenum-graecum.
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