Wetenschappelijk jaaroverzicht 2012 - Catharina Ziekenhuis

clotting time, and a chromogenic ecarin clotting time) on 2 coagulation analyzers. We found
routine tests to be nondiscriminative between concentrations of different DTIs in the aPTT.
Moreover, for PT and TT, the responses for different DTIs differed. This was similar for ecarin
clotting assays. The Hemoclot and HemosIL assays showed identical linear increases for all 3
DTIs. We conclude that dedicated calibrated assays based on a diluted TT (Hemoclot and
HemosIL) appear to be the most suitable for monitoring purposes.
Impactfactor: 2.598
Curvers J
Reference intervals of extended erythrocyte and reticulocyte parameters
Hoffmann JJ, Broek NM van den, Curvers J*
Clin Chem Lab Med. 2012 Mar 2;50(5):941-8
Background: Optical analysis of erythrocytes can provide information on the haemoglobin
concentration and content of reticulocytes and mature erythrocytes. Such parameters have
proven clinical utility in anemia diagnosis and therapy monitoring. For interpretation,
reliable reference ranges are needed. The aim of this study was to establish reference
intervals for extended erythrocyte andreticulocyte parameters as measured with the Abbott
CELL-DYN Sapphire hematology analyzer. Secondary aims were to study sample stability and
to investigate gender- and age dependency of the reference ranges.
Methods: Extended RBC parameters were measured in routine samples of a primary health
care laboratory. The study cohort included 8161 samples of unique individuals, which were
nalyzed using Bhattacharya statistics. As a comparison, reference intervals were calculated in
a subset of individuals without iron depletion.
Results: The majority of erythrocyte and reticulocyte para\xadmeters were normally
distributed, allowing calculation of reference intervals. Only for hypo- and hyperchromic
erythrocytes non-parametric statistics had to be used. The reference range for mean cellular
hemoglobin content of reticulocytes (MCHr) was 28.5-34.5 pg (1.77-2.14 fmol) in the entire
study group and 26.0-35.1 pg (1.60-2.17 fmol) in the non iron-depleted subgroup. No
differences between sexes were found. Most parameters showed significant age effects in
children and adolescents. Conclusions: Reference intervals have been established for
extended RBC and reticulocyte parameters for the CELL-DYN Sapphire. Gender effects could
not be demonstrated and age effects were of limited size, except for individuals younger
than 18 years. Extended RBC parameters are stable for at least 6 h after blood collection.
Impactfactor: 2.150
Curvers J
Trueness in the measurement of haemoglobin: consensus or reference method?
Boonen KJ*, Curvers J*, Timmerman AA, Steurs D, Kerkhof D van de*
Clin Chem Lab Med. 2012;50(3):511-14. Epub 2011 Nov 10
Voor abstract zie: Algemeen Klinisch Laboratorium - Boonen KJ
Impactfactor: 2.150
Heuvel D van den
Verbeterde Cushing speekseldiagnostiek m.b.v. eigen UPLC MS/MS methode die
onderscheid maakt tussen cortisoN en cortisoL
Boer AK*, Heuvel D van den*, Lentjes E
Ned Tijdschr Klin Chem Labgeneesk 2012; 37: 219-221
Impactfactor:--
10