Endoproteinase Asp-N from Pseudomonas fragi ... - Sigma-Aldrich
Endoproteinase Asp-N from Pseudomonas fragi ... - Sigma-Aldrich
Endoproteinase Asp-N from Pseudomonas fragi ... - Sigma-Aldrich
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The <strong>Asp</strong>-N proteolytic fragments were identified as<br />
follows:<br />
Retention Time (min) Mass (Da) Fragment<br />
7.63 731.3 <strong>Asp</strong>(15)-Gln(20)<br />
12.04 863.3 His(1)-Ser(8)<br />
15.65 787.3 <strong>Asp</strong>(9)-Leu(14)<br />
22.92 1152.3 <strong>Asp</strong>(21)-Thr(29)<br />
During the 18 hour digestion only the expected peptides<br />
were generated with no indication of other major<br />
proteolytic activity.<br />
References<br />
1. Drapeau, G.R., J. Biol. Chem., 255, 839-40, (1980).<br />
2. Wilson, K.J. et al., Methods in Protein Sequence<br />
Analysis: Proceedings of the 7th International<br />
Conference, Berlin, July 3-8, 1988, Wittmann-<br />
Liebold, B. (ed.), Springer-Verlag, (New York,<br />
1989), p 310.<br />
3. Ponstingl, H. et al., Advanced Methods in Protein<br />
Microsequencing Analysis, Wittmann-Liebold, B. et<br />
al., (eds.), Springer-Verlag, (New York, 1986)<br />
p 316.<br />
4. Cooper, J.A. et al., J. Biol. Chem., 259, 7835-41,<br />
(1984).<br />
5. Guild, B.C. and Strominger, J.L., J. Biol. Chem.,<br />
259, 9235-40, (1984).<br />
6. Aitken, A. et al., Protein Sequencing: A Practical<br />
Approach, Findlay, J.B.C. and Geisow, M.J. (eds.),<br />
IRL Press, (Oxford, 1989), p 43.<br />
7. Eckerskorn, C. and Grimm, R., Electrophoresis, 17,<br />
899-906, (1996).<br />
8. Scheler, C. et al., Electrophoresis, 19, 918-27,<br />
(1998).<br />
9. Jimenez-Asensio, J., et al., J. Biol. Chem., 274,<br />
32287-94, (1999).<br />
10. Jeno, P., et al., Anal. Biochem., 224, 75-82, (1995).<br />
SIGMA HPLC Purified Products:<br />
Product Package Product<br />
Name Size Code<br />
Carboxypeptidase Y 25 µg C 4046<br />
α-Chymotrypsin 25, 100 µg C 6423<br />
<strong>Endoproteinase</strong> Arg-C 5 µg P 6056<br />
<strong>Endoproteinase</strong> <strong>Asp</strong>-N 2 µg P 3303<br />
<strong>Endoproteinase</strong> Glu-C 25 µg P 6181<br />
<strong>Endoproteinase</strong> Lys-C 5 µg P 3428<br />
Leucine aminopeptidase 50 µg L 9776<br />
Trypsin 100 µg T 8658<br />
Insulin Chain B, Oxidized 100 µg I 1764<br />
Melittin 100 µg M 1407<br />
Glucagon 100 µg G 7774<br />
α-Melanocyte 1, 5 mg M 4135<br />
Stimulating Hormone<br />
Titles of Related Books (Product Code):<br />
MASS SPECTROMETRY OF PROTEINS AND<br />
PEPTIDES: Methods in Molecular Biology (M 7059)<br />
MICROCHARACTERIZATION OF PROTEINS<br />
(M 9807)<br />
NEW METHODS IN PEPTIDE MAPPING FOR THE<br />
CHARACTERIZATION OF PROTEINS (Z36,931-4)<br />
A PRACTICAL GUIDE TO PROTEIN AND PEPTIDE<br />
PURIFICATION FOR MICROSEQUENCING<br />
(Z35,034-6)<br />
SEQUENCING OF PROTEINS AND PEPTIDES:<br />
Laboratory Techniques in Biochemistry and<br />
Molecular Biology, 2nd Ed. (S 2521)<br />
PROTEOLYTIC ENZYMES: A Practical Approach<br />
(P 4926)<br />
PROTEIN STRUCTURE: A Practical Approach<br />
(Z37,548-9)<br />
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY<br />
OF PEPTIDES AND PROTEINS: Separation,<br />
Analysis and Conformation (H 8147)<br />
HPLC OF MACROMOLECULES: A Practical Approach<br />
(H 3409)<br />
PROTEIN SEQUENCING PROTOCOLS: Methods in<br />
Molecular Biology (Z37,395-8)<br />
LKB 6/01<br />
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