PhytoCide Aspen Bark Extract Powder Fact Sheet - Lotioncrafter

BACKGROUND
Populus tremuloides also referred to as Quaking, Trembling or
American Aspen originates in the northern and western areas
of North America. The trees typically grow in vast colonies that
originate from a single seedling. The trees’ primary means of
reproduction is via the sprouting root suckers (new stems) from
the roots. Although the trees live on average between 40 and
150 years, the root system can survive for a considerably longer
period of time. In fact an Aspen colony in Utah referred to as
‘Pando’ is said to be 80,000 years old, which would make this colony
the oldest living colony. The Aspen’s root system is capable
of spreading approximately one meter per year and is therefore
capable of expanding to cover an area of several hectares. One
reason for the tree’s success is due to the ability of the colony to
withstand fires. Although the trees may burn, the roots that are
capable of sprouting new trees are safely below the fire’s reach.
SCIENCE
PhytoCide Aspen Bark Extract Powder
Active Micro Technologies
The bark of the Aspen tree is rich in salicylates that may function
as the plant’s natural defense mechanism against invading parasites.
The salicylates in Aspen bark may also be used for medicinal
purposes. Medicinal barks have been used as analgesics and
to reduce fevers for centuries. Today, doses of 1 to 3 g of bark
with concentrations ranging between 60
to 240 mg of salicylates is recommended
as an analgesic for low back pain.
Technical Data Sheet
Code Number: M16002
INCI Nomenclature:
Populus tremuloides Bark Extract
INCI Status: Approved
REACH Status: Fully Compliant
CAS Number: 999999-99-4
EINECS Number: 310-127-6
Origin: Botanical:
Populus tremuloides
Processing:
GMO Free
No Ethoxylation
No Irradiation
No Sulphonation
No Ethylene Oxide treatment
No Hydrogenation
Additives: None
-Preservatives: None
-Antioxidants: None
Other additives: None
Solvents used: Water
Appearance: Free Flowing Powder
Soluble/Miscible: Fully Water Soluble
Suggested Use Levels: 0.2 - 3.0%
Suggested Applications: Skin Conditioning
& Antimicrobial
Version 6 - 02.10.11
BENEFITS
These salicylates may be isolated from Aspen Bark and applied to cosmetic
and personal care products as a natural alternative to traditional chemical preservative
systems. Our manufacturing method allows us to consistently isolate
a salicylate content between 54.0 – 60.0%, which makes PhytoCide Aspen
Bark Extract Powder an ideal antimicrobial. As an additional benefit Phyto-
Cide Aspen Bark Extract Powder may also impart a smooth feel to the skin.
Page 1 of 2
Active Micro Technologies, LLC - www.activemicrotechnologies.com - info@activemicrotechnologies.com
107 Technology Drive - Lincolnton, NC 28092 - USA - Phone (704) 276-7100 - Fax (704) 276-7101
Information contained in this technical literature is believed to be accurate and is offered in good faith for the benefit of the customer. The company, however, cannot assume any liability or risk involved in the use of its chemical
products since the conditions of use are beyond our control. Statements concerning the possible use of our products are not intended as recommendations to use our products in the infringement of any patent. We make
no warranty of any kind, expressed or implied, other than that the material conforms to the applicable standard specification. Freedom from patent infringement is not implied. All information is for investigative purposes only.

PhytoCide Aspen Bark Extract Powder
Active Micro Technologies
A Double Challenge Test was completed using 2% PhytoCide Aspen Bark Extract Powder in a generic
cream base formulation. Samples were inoculated with E. coli, P. aeruginosa, S. aureus, A. brasiliensis
and C. albicans. During the first 28-day incubation period, samples were periodically collected and
tested for the presence of these microorganisms. Following this initial 28 days of incubation, the cream
samples were then re-inoculated with the microbial cultures and sampled over an additional 28-day period.
Table 1 shows the positive preservation results for PhytoCide Aspen Bark Extract Powder.
E. coli P. aeruginosa S. aureus A. brasiliensis C. albicans
Inoculum
(initial)
Day 0
Day 7
Day 14
Day 21
Day 28
1.2x10 6 2.1x10 4 8.0x10 5 1.3x10 4 1.8x10 4
99.967% >99.999% 99.525% 99.462% 95.444%
>99.999% >99.999% 99.998% 99.385% 99.889%
>99.999%
>99.999%
>99.999%
>99.999%
>99.999%
>99.999%
99.462%
99.846%
>99.999%
>99.999%
>99.999% >99.999% >99.999% 99.769% >99.999%
Inoculum (reinoculated)
1.7x10 6
9.9x10 4 2.1x10 6 2.0x10 5 1.1x10 5
Day 7
Day 14
Day 21
Day 28
>99.999% >99.999% 99.995% 98.500% >99.999%
>99.999% >99.999% >99.999% 98.500% >99.999%
>99.999% >99.999% >99.999% 99.000% >99.999%
>99.999% >99.999% >99.999% 99.850% >99.999%
Table 1. Challenge Test results for 2% PhytoCide Aspen Bark Extract Powder in a cream formulation inoculated on day 0 and
re-inoculated on day 28. Results show % reduction in viable organisms.
USE RECOMMENDATIONS
PhytoCide Aspen Bark Extract Powder is water soluble and may therefore be added to aqueous systems,
as well as the aqueous phase of emulsions. When using PhytoCide Aspen Bark Extract Powder it is recommended
to maintain the formulation pH between 3 and 9. We also suggest incorporating the product at
temperatures of 60°C or below.
Version 6 - 02.10.11
Page 2 of 2
Active Micro Technologies, LLC - www.activemicrotechnologies.com - info@activemicrotechnologies.com
107 Technology Drive - Lincolnton, NC 28092 - USA - Phone (704) 276-7100 - Fax (704) 276-7101
Information contained in this technical literature is believed to be accurate and is offered in good faith for the benefit of the customer. The company, however, cannot assume any liability or risk involved in the use of its chemical
products since the conditions of use are beyond our control. Statements concerning the possible use of our products are not intended as recommendations to use our products in the infringement of any patent. We make
no warranty of any kind, expressed or implied, other than that the material conforms to the applicable standard specification. Freedom from patent infringement is not implied. All information is for investigative purposes only.