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Emerging cytokines and cytokine signalling molecules as ...

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Incubation of full length IL-1F9 with Supernatant from<br />

stimulated neutrophils incre<strong>as</strong>es pro-inflammatory <strong>cytokine</strong><br />

production<br />

IL-6 (pg/ml)<br />

Full length F9 incubated with<br />

SN from activated neutrophils<br />

3000<br />

5min<br />

15min<br />

2000<br />

IL-8<br />

1000<br />

0<br />

ns<br />

NSN<br />

IL-1F9 + NSN<br />

IL-1F9 (100ng/ml)<br />

TNFa (10ng/ml)<br />

TNFa + F9<br />

TNFa + F9 + NSN<br />

• Isolated neutrophils activated with zymosan<br />

• SN incubated 15 min with full length F9<br />

• added to cells

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