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Modern Trends in Human Leukemia III - Blog Science Connections

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Cellular and Virological Studies Directed to the Pathogenesis<br />

19<br />

NPB7-15<br />

GaLV H<br />

/SISAV<br />

/<br />

w<br />

a:<br />

::;)<br />

~<br />

5 NPB7-16<br />

(J<br />

a:<br />

w<br />

CL<br />

(1)<br />

..J<br />

..J<br />

W<br />

(J<br />

W<br />

..J<br />

~ 10 7<br />

:;<br />

GaLV H<br />

/ SiSAV<br />

/<br />

BaEV<br />

10<br />

20 30 40 50 60 70 80<br />

DAYS IN CULTURE<br />

Fig.3. Induction of growth of B-Iymphocytes from normal human blood by primate type-C<br />

retroviruses (GaLVH and SiSA V).<br />

<strong>Human</strong> blood leukocytes were cultured with or without the Hall's Island stra<strong>in</strong> of GaLV or<br />

with the simian sarcoma associated virus (helper virus) (SiSAV). In some cases (e.g., sampie<br />

NPB 7-15 shown <strong>in</strong> the figure) <strong>in</strong>duction of growth was rapid. In other cases (e.g., sampie NPB<br />

7~16 shown <strong>in</strong> the figure lower panel) growth <strong>in</strong>duction occurred after 50 days. Spontaneous<br />

growth (no virus) occurred <strong>in</strong> only 1 to 2% of sampies but <strong>in</strong> more than 40% of cells treated<br />

with virus if a co-cultivation method was used (P. Markharn, F. Ruscetti. Z. Salahudd<strong>in</strong>,<br />

R. Gallagher, and R. Gallo, <strong>in</strong> press).

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