Modern Trends in Human Leukemia III - Blog Science Connections

More documents

Recommendations

Info

Cellular and Virological Studies Directed to the Pathogenesis 15 bution of samples is virtually identical with DNA from normal and leukemic cells. In contrast, using BaEV probes we find a small number of leukemic DNA samples which hybridize significantly more of the probe (data not shown). We have not conducted an extensive survey for BaEV related sequences using the more sensitive approach of restriction endonuclease- Southern blotting, but we have applied it to a few very select cases and verified the higher hybridization obtained in the molecular hybridization survey. These results were mentioned above and presented with AML A. Hybridlzatlon of SiSV 3~-CDNA to Human DNA Normal [32 samples) a Lymphocytic Leukemla 114 samples] 0 AML 119 samolasl A CML 113 : CML 'Blast Crisir [I2 0.6.0 6.1-12.0 12.1-16.0 16.1 -24.0 ---- - % Hybridization n 2 a E B. Hybrldlzatlon of SiSV I-RNA to Human DNA ~ormal 122 samp~es~ d A Lymphocytic Leukemla 117 samples] o AML [14 samples] A CML 114 samples] CML Blast Crisls 115 samples] 44.0 4.1-8.0 8.1-120 12.1-16.0 % Hybridlatlon Fig. 2. Survey of human DNA from normal blood and from people with various types of leukemia for simian sarcoma virus (woolly monkey) (SiSV) related sequences. In the upper panel (A) the probe used was 3H-cDNA prepared from endogenous reverse transcriptase reactions. In the lower panel (B) the probe was 1125 labeled 70s RNA. In both cases the vast majority of DNA samples from normal tissues hybridized less than 8% and all but a few less than 16%. In contrast. a significant percent of leukemic samples hybridized more than 12% and several more than 16%. Again, these results do not discriminate between SiSV sequences and SiSV related sequences. This survey was carried out by M. Reitz and N. Miller in our group.
16 Gallo, R. C. patients HL-49 and HL-23 elsewhere in this book [49]. If the survey results are a true indication of the presence of these sequences then we can safely rule out BaEV or a closely related virus as commonly associated with human leukemia and therefore as a common cause of human leukemia. However. it may be important to keep in mind that the results could be underestimations, e.g., if only fragments of provirus are present and/or only a small number of cells in a population contain viral sequences, the sequences might easily be undetected, but possibly sufficient for leukemic transforma tion. We also surveyed DNA purified from many human tissues for SiSV related sequences, and as illustrated in Fig. 2 severalleukemic DNA sampIes hybridized more nucleic acid probes ofSiSV (grown in marmoset cells) than did DNA from normal tissues. This is more clearly evident when one compares the distribution ofhybridization ofviral prob es to various human DNA sampies between rat virus (Fig. 1) and SiSV (Fig. 2). However, one DNA sampie from anormal tissue (one of six human placentas tested) also showed unusually high hybridization to SiSV probes, so c1early higher hybridization is not unique to certain leukemic cells. To verify these high er hybridization results and to obtain greater sensitivity, some of these sampIes were also analyzed by restriction endonuc1ease digestion-Southern blotting, and hybridization of the specific fragments to SiSV 35S RNA. As described elsewhere in this book (49], the positive results with the one placenta derived from a normal person (termed NP3) obtained in the standard molecular hybridization survey was confirmed as was one leukemic sampie. In these cases it is difficult to escape the conc1usion of infection by an SiSV related virus. The presence in placenta is particularly interesting since the finding suggests the possibility of congenital virus .transmission, a mechanism apparently involved in natural virus transmission seen in chickens (44], cats [19], cattle (26], and gibbon apes [23]. The data are also consistent with the isolation of an SiSV related virus from a human embryo by Panem and Kirsten (32]. As noted earlier SiSV and GaLV are not endogenous to primates but present in them only after infection. Although their recent history (? vectors) is unknown their ancestral origin is believed to be rodents. An unexpected observation, that came out of the search of human DNA for inserted viral sequences related to SiSVand GaLV was the finding of an Si SV related sequence in all human DNA. This was detected after digestion of the DNA with BAM 1, with HIND III, and with Xpa restriction endonuc1eases (see paper by F. Wong-Staal et al. elsewhere in this book). This seq uence was specific to this virus group since no endogenous viral sequence (i.e. sequences in DNA from normal uninfected tissues from various cell sampies) were detected with probes from other viruses. We found this sequence using either SiSV (SiSA V) or SiSAV, and the results were obtained with viruses grown in non-human cells. Therefore, the results cannot be due to a trivial explanation such as presence of human cellular sequences with the viral probe. Moreover, it appears that this is not an evolutionarily conserved sequence since it was not found in the DNA we
Page 1 and 2: Haematology and Blood Transfusion 2
Page 3 and 4: Dr. Rolf Neth, Universitäts-Kinder
Page 5 and 6: Participants of the Meeting Adamson
Page 7 and 8: Participants of the Meeting xv Knig
Page 9 and 10: Wilsede Scholarship Holders (grante
Page 11 and 12: xx Preface
Page 13 and 14: Acknowledgement We should like to t
Page 15 and 16: 4 Moloney. W. C. Fig.l Fig.2
Page 17 and 18: 6 Moloney. w. C. Fig.5 Fig.6 genesi
Page 19 and 20: 8 Gallo. R. C. though the age of ea
Page 21 and 22: Table 1. Infectious primate type-C
Page 23 and 24: 12 Gallo, R. C. some viruses and, i
Page 25: Gallo. R. C. A. Hybridlzatioq ot Mu
Page 29 and 30: 18 GaIlo, R. C. conc1ude that the m
Page 31 and 32: 20 Gallo. R. C. Fig.4. Morphologyof
Page 33 and 34: 22 Gallo. R e. lespie. D. H .. Reit
Page 35 and 36: 24 Gallo, R e. 46. Wolfe, L. G., De
Page 37 and 38: 26 Pinkel, D. therapy; meningeal ir
Page 39 and 40: 28 Pinkel. D. .!2.. 150 ...ll. 140_
Page 41 and 42: 30 Pinkel, D. failure to eradicate
Page 43 and 44: 32 PinkeL D. kemia cells and theref
Page 45 and 46: Epidemiology of Leukemia Miller, R.
Page 47 and 48: Epidemiology of Leukemia 39 In the
Page 49 and 50: Epidemiology of Leukemia 41 12. Fun
Page 51 and 52: 44 Rowley, J. D. contain the PhI ch
Page 53 and 54: 46 Rowley, J. D. In regard to the s
Page 55 and 56: 48 Rowley, J. D. icantly related to
Page 57 and 58: 50 Rowley, J. D. mosomes with the g
Page 59 and 60: 52 Rowley, J. D. 28. Rowley, 1. D.:
Page 61 and 62: 54 Fialkow. P. J. cludes any hypoth
Page 63 and 64: 56 Fialkow, P. J. using G-6-PD as a
Page 65 and 66: 58 Fialkow, P. 1. 14. Maniatis, A.K
Page 67 and 68: 60 Georgii. A. For these reasons th
Page 69 and 70: 62 Georgii, A.
Page 71 and 72: 64 Georgii, A.
Page 73 and 74: 66 Georgii, A. Table 3. Values of c
Page 75 and 76: 68 Georgii. A. chronic granulocytic
Page 77 and 78:
70 Georgii, A. Rappaport, H.: Acute
Page 79 and 80:
72 Gale, R. P. The human major hist
Page 81 and 82:
74 Gale, R. P. Approximately 60-70
Page 83 and 84:
76 Gale. R. P. Table 2. Leukemic re
Page 85 and 86:
78 Gale, R. P. I I. Neiman, P. E.,
Page 87 and 88:
80 Bekesi. J. G .. Holland. J. F. a
Page 89 and 90:
82 Bekesi. 1. G .. Holland. J. F. u
Page 91 and 92:
84 Bekesi. J. G .. Holland. 1. F. p
Page 93 and 94:
Bekesi. J. G.. Holland, J. F. 2 - X
Page 95 and 96:
Treatment of Adult Acute Myeloblast
Page 97 and 98:
Treatment of Adult Acute Myeloblast
Page 99 and 100:
Prediction of Therapeutic Jlesponse
Page 101 and 102:
Prediction ofTherapeutic Response i
Page 103 and 104:
Prediction ofTherapeutic Response i
Page 105 and 106:
Prognostic Factors in Adult Acute L
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
108 Catovsky. D. et al. Results The
Page 115 and 116:
110 Catovsky. D. et al. Table 1. Mo
Page 117 and 118:
112 Catovsky, D. et al. clear outli
Page 119 and 120:
Intermediate Dose Methotrexate (IDM
Page 122 and 123:
118 Freeman. A. I. et al. again at
Page 124 and 125:
120 Freeman. A. I. et al. c: 90 o .
Page 126 and 127:
122 Freeman. A I. et a!. A comparab
Page 128 and 129:
122 Freeman. A. I. et al. A compara
Page 130 and 131:
Marrow Terminal Deoxynucleotidyl Tr
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Clinical Significance ofTdT, Cell S
Page 138 and 139:
CIinical Sigmificance ofTdT. Cell S
Page 140 and 141:
Clinical Significance ofTdT. Cell S
Page 142 and 143:
Clinical Significance ofTdT, Cell S
Page 144 and 145:
Biochemical Determinants for Antile
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
146 Chandra, P. et al. CYTOSINE o .
Page 152 and 153:
148 Chandra, P. et al. 700 600 I 50
Page 154 and 155:
150 Chandra, P. et al. 120 ~100 c:
Page 156 and 157:
152 Chandra. P. et al. 100 000 ..,.
Page 158 and 159:
154 Chandra, P. et al. WBC ~ MPC 0.
Page 160 and 161:
Summary of Clinical Poster Sessions
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Progress in Acute Leukemia 1975-197
Page 168 and 169:
Page 170 and 171:
Page 172 and 173:
Regulatory Interactions in Normal a
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
Page 180 and 181:
Page 182 and 183:
Leukemic Inhibition ofNormal Hemato
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Clonal Diseases of the Myeloid Stem
Page 194 and 195:
Clonal Diseases of the Myeloid Ster
Page 196 and 197:
Clonal Diseases ofthe Myeloid Stern
Page 198 and 199:
Clon al Diseases of the Myeloid Ste
Page 200 and 201:
200 Greenberg. Po. Mara. B. granulo
Page 202 and 203:
202 Greenberg. P .. Mara. B. rates
Page 204 and 205:
204 Greenberg. P .. Mara. B. Heller
Page 206 and 207:
206 Adamson. J. W. hibited only iso
Page 208 and 209:
208 Adamson. J. W. endogenous colon
Page 210 and 211:
210 Adamson. J. W. Acknowledgments
Page 212 and 213:
212 Biermann. E. et al. Table 1. Cy
Page 214 and 215:
214 Biermann. E. et al. Table 3. Qu
Page 216 and 217:
Differentiation Ability of Peripher
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
224 Dexter. T. M .. Teich. N. M ..
Page 224 and 225:
Table 3. Biological effects ofinfec
Page 226 and 227:
228 Dexter, T. M., Teich. N. M. "CF
Page 228 and 229:
Characterization of Myelomonocytic
Page 230 and 231:
Characterization of MyeIomonocytic
Page 232 and 233:
Characterization of Myelomonocytic
Page 234 and 235:
238 Yefenof. E. Using the quantitat
Page 236 and 237:
242 Duesberg. P. et aL all, or part
Page 238 and 239:
244 Ouesberg. P. et al. 2 A (e) MC2
Page 240 and 241:
Duesberg. P. et a!. C 345 MC29{MCAV
Page 242 and 243:
248 Ouesberg. P. et al. Table 2. Tr
Page 244 and 245:
2S0 Duesberg. P. et al. Table 5. T
Page 246 and 247:
252 Ouesberg. P. et al. Table 6. Hy
Page 248 and 249:
254 Duesberg. P. et al. A c . 12 13
Page 250 and 251:
256 Duesberg. P. et al. A B C 0 P 1
Page 252 and 253:
258 Duesberg. P. et al. In vitro tr
Page 254 and 255:
260 Duesberg. P. et al. 5. Hu. S. S
Page 256 and 257:
262 Erikson. R. L. et al. The major
Page 258 and 259:
264 Erikson. R. L. et al. Virus p60
Page 260 and 261:
266 Erikson. R. L. et al. - 88K- Pr
Page 262 and 263:
268 Erikson. R L. et al. concerning
Page 264 and 265:
The in vitro Translation of Rous Sa
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Regulation of Translation of Eukary
Page 272 and 273:
Regulation ofTranslation ofEukaryot
Page 274 and 275:
Regulation ofTranslation of Eukaryo
Page 276 and 277:
284 Kramer. G. et al. [ e1F-2 Mel-t
Page 278 and 279:
286 Kramer. G. et al. Translational
Page 280 and 281:
288 Kramer, G. et al. A B - 1234567
Page 282 and 283:
290 Kramer, G. et al. 22. Levin, D.
Page 284 and 285:
292 Kerr, I. M. et al. that we and
Page 286 and 287:
294 Kerr. I. M. et al. being suffic
Page 288 and 289:
296 Koch, G. et al. Labeling Of Cel
Page 290 and 291:
298 Koch, G. et al. the RTE of the
Page 292 and 293:
300 Koch. G. et al. zidine techniqu
Page 294 and 295:
Inhibition of Polypeptide Chain Ini
Page 296 and 297:
Inhibition of Polypeptide Chain Ini
Page 298 and 299:
Possible Role of the Friend Virus L
Page 300 and 301:
Possible Role of the Friend Virus L
Page 302 and 303:
Possible Role ofthe Friend Virus Li
Page 304 and 305:
314 Jones, C. Fig. 2. Demonstration
Page 306 and 307:
316 Jones. C. Table 1. Characterist
Page 308 and 309:
Effect of Vitamin A on Plasminogen
Page 310 and 311:
Effect ofVitamin A on Plasminogen A
Page 312 and 313:
Effect ofVitamin A on Plasminogen A
Page 314 and 315:
326 Hardesty. B. Specific m RNA New
Page 316 and 317:
328 Hardesty, B. of their "transfor
Page 318 and 319:
330 Hardesty. B. cytes is held in a
Page 320 and 321:
332 Hardesty. B. the normal control
Page 322 and 323:
336 Greaves. M. F. Table 1. Markers
Page 324 and 325:
338 T Li neage Tdt+ ALL+ 1 - T+ Pre
Page 326 and 327:
340 Greaves. M. F. or 'Ia'-like ser
Page 328 and 329:
342 Greaves. M. F. Table 3. Acute l
Page 330 and 331:
344 Greaves, M. F Catovsky, D.: Mem
Page 332 and 333:
Characterization of Antigens on Acu
Page 334 and 335:
Page 336 and 337:
Page 338 and 339:
Common All Associated Antigen from
Page 340 and 341:
Page 342 and 343:
Page 344 and 345:
Page 346 and 347:
Page 348 and 349:
Page 350 and 351:
366 .. 0 . . ' 't ••• b c d B
Page 352 and 353:
368 Brown, Go et al. Table 3. Genet
Page 354 and 355:
370 Bach. F. H. et al. carry the Ly
Page 356 and 357:
372 Bach, F. H. et al. dividual B i
Page 358 and 359:
374 Bach. F. H. et al. Table 4. Lys
Page 360 and 361:
376 Bach, F. H. et aI. 8. Solliday,
Page 362 and 363:
378 Oliver. R. T. 0 .. Lee. S. K. g
Page 364 and 365:
T Cell Function in Myelogenous Leuk
Page 366 and 367:
T Cell Function in Myelogenous Leuk
Page 368 and 369:
Recognition of Simian Sarcoma Virus
Page 370 and 371:
Page 372 and 373:
Page 374 and 375:
Recognition ofSimian Sarcoma Virus
Page 376:
Recognition ofSimian Sarcoma Virus
Page 379 and 380:
396 Snyder, H. W .. Je. et aL 100 2
Page 381 and 382:
398 Snyder, H. W., Jr. et al. the g
Page 383 and 384:
A Search for Type-C Virus Expressio
Page 385 and 386:
A Search for Type-C Virus Expressio
Page 387 and 388:
Tumor Cell Mediated Degradation In
Page 389 and 390:
Tumor CeH Mediated Degradation In V
Page 391 and 392:
Tumor Cell Mediated Degradation In
Page 393 and 394:
Evidence Supporting a Physiological
Page 395 and 396:
Evidence Supporting a Physiological
Page 397 and 398:
418 Witz, 1. P. to detect the corre
Page 399 and 400:
420 Witz. I. P. against antigens as
Page 401 and 402:
424 Weiss, R. A. Thus retroviruses
Page 403 and 404:
426 Weiss, R. A. 60000 daltons that
Page 405 and 406:
Mechanism of Leukemogenesis and of
Page 407 and 408:
Mechanism ofLeukemogenesis and ofTa
Page 409 and 410:
Mechanism of Leukemogenesis and ofT
Page 411 and 412:
Page 413 and 414:
Page 415 and 416:
Infectious Leukemias in Domestic An
Page 417 and 418:
Page 419 and 420:
Page 421 and 422:
Biochemical and Epidemiological Stu
Page 423 and 424:
Page 425 and 426:
Page 427 and 428:
Page 429 and 430:
Leukemia Specific Antigens: FOCMA a
Page 431 and 432:
Page 433 and 434:
Page 435 and 436:
Page 437 and 438:
Page 439 and 440:
Page 441 and 442:
Page 443 and 444:
466 Essex. M. et a!. M .. Cotter. S
Page 445 and 446:
A Single Genetic Locus Determines t
Page 447 and 448:
Page 449 and 450:
Page 451 and 452:
Page 453 and 454:
A Single Genetic Locus Oetermines t
Page 455 and 456:
Page 457 and 458:
Page 459 and 460:
484 Hillova. J .. Hill. M. Wong-Sta
Page 461 and 462:
486 Hillova. J .. HilI. M. Referenc
Page 463 and 464:
488 Teich. N. et al. Abelson virus
Page 465 and 466:
490 Teich. N. et a!. these cell typ
Page 467 and 468:
492 Nooter. K. et al. Table 1. Immu
Page 469 and 470:
494 Nooter. K. et al. (6 of 17). Of
Page 471 and 472:
496 Nooter. K. et al. human embryon
Page 473 and 474:
498 Chandra. P. et al. Table 1. DNA
Page 475 and 476:
500 Chandra. P. et al. References 1
Page 477 and 478:
502 Jensen. F. C. ity. before and a
Page 479 and 480:
The Role of Gene Rearrangement in E
Page 481 and 482:
The Role ofGene Rearrangement in Ev
Page 483 and 484:
The Role ofGene Rearrangement in Ev
Page 485 and 486:
514 Cornelis. G. and, later, transf
Page 487 and 488:
516 Cornelis. G. Fig. 2. Heterodupl
Page 489 and 490:
518 Cornelis. G. D. Structure of th
Page 491 and 492:
520 Cornelis. G. .Nllat 27.8 414 pG
Page 493 and 494:
The Virus-Cell Gene Balance Model o
Page 495 and 496:
Page 497 and 498:
Page 499 and 500:
530 Haseltine, W. A. et al. 5' 3' -
Page 501 and 502:
532 Haseltine, W. A. et al. A . .
Page 503 and 504:
534 Haseltine. W. A. et al. • A B
Page 505 and 506:
Haseltine, W. A. et al. - m 0 B Akv
Page 507 and 508:
538 Haseitine, W. A. et al. from th
Page 509 and 510:
540 Haseltine. W. A. et al. • •
Page 511 and 512:
542 Haseltine, W. A. et al. A Wl,.V
Page 513 and 514:
Comparative Analysis ofRNA Tumor Vi
Page 515 and 516:
548 • • c a b .... .' .. " -•
Page 517 and 518:
550 Haseitine. W. A. et al. Table 4
Page 519 and 520:
552 Haseltine, W. A. et al. Essex,
Page 521 and 522:
554 Wong-Staal. F. et al. ofthe spl
Page 523 and 524:
556 Wong-Staal. F. et aJ. a b c d e
Page 525 and 526:
558 Wong-Staal. F. et al. Detection
Page 527 and 528:
560 Wong-Staal. F. et al. ing HL23,
Page 529 and 530:
562 Doehmer. J. et al. endogenous v
Page 531 and 532:
564 Doehmer. J. et al. Table 1. Cor
Page 533 and 534:
566 Doehmer. J. et al. Table 3. Seg
Page 535 and 536:
568 Doehmer.1. et al. integration s
Page 537 and 538:
570 Bacheier. L. T.. Fan. H. A 8 C
Page 539 and 540:
572 Bacheier. L. T.. Fan. H. ABCDEF
Page 541 and 542:
Release of Particle Associated RNA
Page 543 and 544:
Release of Particle Associa ted RNA
Page 545 and 546:
Release ofParticie Associated RNA D
Page 547 and 548:
582 Boccara. M .. Kelly. F. Table 1
Page 549 and 550:
584 Boccara. Mo. Kelly. F strain) a
Page 551 and 552:
Summary of Meeting on Modem Trends
Page 553 and 554:
Summary of Meeting on Modern Trends
Page 555 and 556:
Subject Index Abelson virus, 425 -
Page 557 and 558:
Subject Index - ofCFU-C, 211 Cytidi
Page 559 and 560:
Subject Index - treatment with, 101
Page 561 and 562:
Subject Index - promoting of, by vi
Page 563:
Organ der Deutschen Gesellschaft f
show all

Modern Trends in Human Leukemia III - Blog Science Connections

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?