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although interspecies reactivity has been detected with the ELISA in some cases, it has not been demonstrated so regularly by ELISA as by competition RIA. It has not yet been determined whether this peculiarity is a property of the ELISA test itself or a consequence of our non-denaturing purification procedure of the proteins tested (electrofocusing, no SDS). The detection with the ELISA-technique of antigens cross-reacting with primate C-type viral pro teins in human leukemias is consistent with the earlier detection of viral structures (reverse transcriptase, RNA, virus-like particles, C-type virus es ) in human leukemic cells with other methods (Gallo et al. 1975; Hehlmann 1976; Gallo et al. in this volume), although previous reports on antigens crossreacting with viral structural proteins have been sporadic and not convincing (Sherr and Todaro 1975) and have been challenged (Stephenson and Aaronson 1976). Reports on the presence of anti-p30 antibodies in human sera (Herbrink et al. 1980) and of anti-reverse transcriptase antibodies on the surface of leukocytes from patients with AML and CML blast crisis (Jacquemin et al. 1978) have to be judged cautiosly because of the known existence of heterophil antibodies (Barbacid et al. 1980; Kurth and Mikschy 1978; Snyder and Fleissner 1980). Tbe failure to reproduce the human ELISA results with human sera with the RIA competition technique points to the possibility that some unrelated but cross-reacting antigens have been detected. Alternatively, the ELISA bin ding assay might recognize p30-related antigens of low avidity not detected by the more stringent RIA competition assay. To decide on these alternatives the nature of the detected antigens is under further investigation. Tbe observation that the presence of crossreacting antigens is associated with leukemia or with shorter survival suggests that regardless of their etiologic role, these antigens might be of prognostic use. Acknowledgments The technical assistance of E. Jatho and S. Ireland is acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 51) and by Euratom (contract 218-76-1 BIAD). References Barbacid M, Bolognesi D, Aaaronson SA (1980) Humans have antibodies capable of recognizing oncoviral glycoproteins: Demonstration that these antibodies are formed in response to cellular modification of glycoproteins rather than as consequence of exposure to virus. Proc Natl Acad Sci USA 77:1617-1621 - Engvall E, Carlsson HE (1976) Enzyme-linked immunosorbent assay, ELISA. Immuno enzymatic techniques. EIsevier/North-Holland, Amsterdam Oxford, pp 135-147 - Gallo RC, Gallagher RE, Miller NR, Mondal H, Saxinger WC, Mayer RJ, Smith RG, Gillespie DH (1975) Relationships between components in primate RNA tumor viruses and in the cytoplasm of human leukemic cells: Implications to leukemogenesis. Cold Spring Harbor Symp Quant Biol 933-961 - Hehlmann R (1976) RNA tumorviruses and human cancer. Curr Top Microbiol Immunol 73: 141-215 - Hehlmann R, Schetters H, Erfle V (to be published) ELISA detects antigens in human leukemic sera that cross-react with primate C-type viral p30-proteins - Herbrink P, Moen JET, Brouwer J, Warnaar SO (1980) Detection of antibodies cross-reactive with type-C RNA tumor viral p30 protein in human sera and exudate fluids. Cancer Res. 40:166-173 - Jacquemin PC, Saxinger C, Gallo RC (1978) Surface antibodies of human myelogenous leukemia leukocytes reactive with speeific type-C viral reverse transcriptases. Nature 276: 230-236 - Kurth R, Mikschy U (1978) Human antibodies reactive with purified envelope antigens of primate type-C tumor viruses. Proc Natl Acad Sei USA 75:5692-5696 - Mesa-Tejada R, Keydar I, Ramanarayanan M, Ohno T, Fenoglio C, Spiegelman S (1978) Immunohistochemical detection of a cross-reacting virus antigen in mouse mammary tumors and human breast careinomas. J Histochem Cytochem 26:532-541 - Nakane PK, Kowaoi A (1974) Peroxidase-Iabelled antibody, a new method of conjugation. J Histochem Cytochem 22: 1084-1091 - Ohno T, Mesa-Tejada R, Keydar I, Ramanarayanan M, Bausch J, Spiegelman S (1979) The human breast careinoma antigen is immunologically related to the polypeptide of the group-speeific glycoprotein of the mouse mammary tumor virus. Proc Natl Acad Sei USA 76:2460-2464 - Parks WP, Scolnick EM (1972) Radioimmunoassay of mammalian type-C viral proteins: Interspeeies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sei USA 69: 1766-1770 - Schetters H, Hehlmann R, Erfle V, RamanarayananM (1980) The detection and quantification of C-type viral proteins in tissues and sera with an enzyme immunoassay. Infect Immun 29:972 - Schetters H, Hehlmann R, Erfle V (1981) ELISA for the detection and quantification of C-type viral glycoprotein (gp70) using antibodies that recognize the protein moieties of the glycoprotein J Virological Methods, in press - Sherr CJ, Todaro GJ (1975) Primate 535
type-C virus p30 antigen in cells from humans with acute leukemia. Seience 187:855-857 - Snyder HW Jr, Fleissner E (1980) Speeifieity of human antibodies to oncovirus glycoproteins: Recognition of antigen by natural antibodies directed against carbohydrate structures. Proc Natl Acad Sei USA 77:1622-1626 - Stephenson JR, Aaronson SA (1976) Search for antigens and antibodies cross-reactive with type C viruses of the woolly monkey and gibbon ape in animal models and in humans. Proc Natl Acad Sei USA 73:1725-1729 - Strand M, August JT (1973) Structural proteins of oncogenic ribonucleic aeid viruses interspec H, a new interspeeies antigen. J Biol Chem 248: 5627-5633 - Voller A, BidweH DE, Bartlett A (1976) Enzyme immunoassays in diagnostic medieine. BuH WHO 53: 55-65 536
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Haematology and Blood Transfusion 2
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Dr. Rolf Neth, Universitäts-Kinder
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Participants of the Meeting Aaronso
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Kurth, R, Friedrich-Miescher-Labora
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Wilsede Scholarship Holders (Grante
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Preface Gut ist eine Lehrart, wo ma
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Personal and scientific discussion
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Acknowledgement We should like to t
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Wilsede, June 21 1978 Memorial Trib
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limiting benign lymphoproliferative
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this most unlikely, however (for re
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longer subject to the same control
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Bregula U, Wiener F, Harris H (1971
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fresh lymph node biopsies from ten
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Fig. 3. Binucleate mitosis in an ob
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y Fig. 4. Schematic diagram of post
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than that among patients with Stage
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Tests of binding affinity, agglutin
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N Engl J Med 297: 963-968 - Green I
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Clinical Aspects
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"ring chromosome". "Mar" is marker,
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191 chromosomally abnormal patients
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tumors of both African and non-Afri
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Haematology and Blood Transfusion V
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Retrospective group 26/93 evolved l
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Page 53 and 54:
Treatment
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advantage in terms of duration of f
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leukaemia. An analysis of 168 patie
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11. Treatment Program Chemotherapy
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efractory congestive heart failure
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period (Sequence II) was gene rally
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children with a multiple drug proto
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DAYS 1 5 10 15 20 25 30 35 , I I I
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Biologically Drug Outcome fit" sens
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may counterbalance the potential be
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Ht (0--0) 50 40 30 20 10 0 Platelet
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10 7 ,-----------------------------
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11. Haematological and Biochemical
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0\ 0\ INTERFERON ... 10 3 rl E "'
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("pre-B" phenotype) has been descri
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human bone marrow cells exhibit the
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10 9.,0 MBKCC .. .,4 B prot:oool 1'
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demann W, Büehner T, Arlin Z, Gee
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-...l 00 a-tLDRENS CAt«:ER S TU>Y
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prognostic characteristics under in
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significantly higher in HR} (14.5%,
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normal or elevated immunoglobulin l
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References Andreeff M, Darzynkiewic
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R GROUP 1 ( 1970 - 1971 ) 17 PTS. R
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Table 3. Influence of initial featu
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LIJ (f) a.. < -1 LIJ Q: u. 0 >- I-
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me nt indicated that additional the
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42:2123-2134 - Chessells JM, Cornbl
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Baum et al. 1979). This study was b
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1. 0 '--'1--" 0.9 e: o .- VI VI E C
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e: o e: 1. 0 ,.--...-- 1'"'\ ......
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In conclusion, we can state that th
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Haematology and Blood Transfnsion V
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INDUCTION VCR + PRED + ASNASE (wk 3
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SCHED.INT 1 MTX • ! Adria MTX •
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~ looh---------ooooc>----o---c>----
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Consoll- Irra .tlon t) .tlon Indu
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Highrisk Standard risk Table 1. Cli
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Cumulo/ive comp/el9 remission 1.0 .
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10° 8 = 6 ö 4 Non T Cell .~ :ö
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% SURVIVAL 100 ~ ~II 0 80 60 40 - -
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a) Percentage of donor cell mitoses
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Table 5. Results of bone marrow tra
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...... w N Table 7. Clinical result
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agent had to be added and that in t
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C. Chronic Myelogenous Leukemia The
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Patients with a suitable donor rece
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No. Recurrent Leukaemia Other death
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followed by dialysis against isoton
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HS, HL- Heme >_ 40 0 HS I ;:. =====
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knowing at present. These genes may
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esistance to antifolates. In Vitro
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a DNA probe specific for the gene t
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translocation between chromosomes 9
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Table 1. Subdivision of 1827 Myelo-
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NORMAL CELL ®
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some change of the primary type, in
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F. Transformation of Mouse Bone Mar
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were carried out with 3H-MTX in the
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the lysozyme cDNA prepared from ovi
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et al. 1976). The BJAB cellline in
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dalton bands corresponding to light
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1979). The EBV-carrying cell lines
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producer lines (DAUDI and P3HR-1),
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G, Giovanella B, Westman A, Stehlin
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Dc;bt CT + i J.I9 Raji; 1. i .. 100
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c. Discussion During infectious mon
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C. Nonepisomal Viral DNA We have us
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A 260 3.0 A B 1670 70 N2 2.0 c: E .
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vivo. Nature 260:302-306 - Kirk JTO
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Fig. 1. a Polyacrylamide gelelectro
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1979). The lymphocyte subpopulation
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lysin 0 antigens are found, and mix
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Pope JH (1978) Long-term T cell-med
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Charaeteristie Morphologie maturati
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after TPA-induction (Table 3). The
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PA (1978) Reactivity of a rabbit an
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Blood Monocyte. B lood Monocytes 1-
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and nonhistone proteins can serve a
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The findings of the evaluation of s
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Fig. 3. Tumor grawth of L 428 cells
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Cell biological and Immunological A
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consideration. Sanel (1973) obtaine
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endotoxin serum, were used. A parti
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monocytic leukemia cell line in cul
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B. Isolation, Characterization and
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don al hemopathies generally displa
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nitors. Blood Cells 6:595-607 - Min
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\I) ....I ....I IU Jl 200 r 100 90
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their haemopoietic cells' ability t
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normal normal ALL ALL ALL persons p
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64.5 { V)
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References Biermann E, Neth R, Gros
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ehambers, the growth pattern observ
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References Anderssen LC, 10kinen M,
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REH CELL UNE TO CALLb~Ö ~q~ V.M. c
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the macrophage series in which the
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Table 1. Production of factor depen
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Table 1. Characteristics of the adh
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Fig. 3. A human marrow culture at 6
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300 250 • HYPERPROLIFERATIVE PATT
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Fig. 9. Nonadherent population from
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The cobblestone areas were often no
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L (1976) Induction of colony format
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B. Materials and Methods J. Tissue
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Fig. 2. Ultrastructural appearance
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Page 307 and 308:
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Table 1. Monoclonal antibody reacti
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al. 1979; Janossy et al. 1979) is e
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to which this is an exact replica o
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inger JL (1976) Distribution of la-
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I region antigens were found to be
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have been reported to be present on
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V. FunctionalAssays Assays for PHA
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Functional studies of UCHT1 separat
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40 ,...... (5 L.. C o u 30 .9 ~ .~
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with immunohistochemieal methods (L
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can be dissected by cloning. Utiliz
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Table 1. Origin and marker profile
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complete identity of gp 100 from no
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Table 1. Reaction of single anti se
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media. Following 24-h incubation, c
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nonleukemic eells (Greaves 1979). L
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Fig. 2. Cytocentrifuged smears of b
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Modification of amino-groups of hum
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disturb this balance in the directi
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The majority of human blood lymphoc
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of the EBV infected B cells. Howeve
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and immunoglobulins. J Immunol 120:
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" K 10 • j ~ ALL RNlL AL wtfh les
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tion akuter Leukämiezellen in "spo
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D. Results and Discussion J. Acute
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selectively responsive to different
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l1J ~ 100 ~ Q. ::) l1J Z 0 ~ z >- .
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Table 2. Growth inhibition of S49 e
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Reverse Infectious ASC/2X1Q6 transc
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munized animals to 1316 tumor cells
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Fig. 3. Expression of retroviral gp
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pg70. J Exp Med 143: 151-166 - McGr
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Table 1. Expression of tumor antige
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Virological and Molecularbiological
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Table 1. Oncogenic properties of re
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also Fig. 1). It followed that the
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whether this sequence is related to
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zed in infected cells argue that th
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a specific viral transforming prote
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detect viral RNA as the only charac
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Eisen H (1980) Myeloproliferate vir
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p,60- -34K - 1 2 3 4 Fig. 1. In vit
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.. p - • a'T ... I .t'l Fig. 4. D
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Fig. 2. Photomicrographs of NY68 in
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DEAE (o(lJmn 'liI GI -2 2 o e s 1
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vity was deterrnined. For endogenou
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pr 180 - - - P 12/15 - A B c D E F
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85,- 66- -27 - -66 40- 27- -1'9 19-
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= 0.5 o E Q. o ILI 0.4 (J) « ILI .
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Table 1. Oncogenic potential of avi
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100 III GI c: o "8 80 > w
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Expt. No. Infecting virus Proportio
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Haematology and Blood 'fransfusion
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sequences, provided its gag portion
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77:3009-3013 - Hu SSF, Moscoviei C,
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RI 2.2 md and Hind III 2.6 md leuke
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RELATIONSHIP OF ENDOGENOUS AND EXOG
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E ........ E Cl. U E :::l .... Q) (
Page 447 and 448:
References Astrin S (1978) Endogeno
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the same animal were examined (e.g.
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I) 'IQ a \0 - CL -- Fig. 2. Restrie
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~ ..................... ~ Cell 3' 5
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a :Sa,C! + . ~ 111 i' ( " '~ .J. b
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Table 1 Sampie Tissue TS fragment"
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a b Sac I / rep ._-_.-_ K J nl rep
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Haematology and Blood 'fiansfusion
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Osteopetrosis and osteosarcomas occ
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Lymphoma cellline Table 1. T and B
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Table 2. In vivo growth and oneogen
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Table 1. Transforming aetivity of c
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Table 3. Transformation aetivity of
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- Shoemaker C, Goff S, Gilboa E, Pa
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indieate a shifting of target eell
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Table 2. Expression of Jl and Sourc
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~ i ,..1---------,,':::::::::::::::
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fragment of pBR322 DNA, a 3.0-kbp S
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Mak TW (1979) Proc Natl Acad Sci US
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Table 1. Transformation of 3T3 cell
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inserts in the genome of rapidly tr
Page 493 and 494: pulations, it seemed likely that th
Page 495 and 496: contains antigens which react with
Page 497 and 498: Haematology and Blood Transfusion V
Page 499 and 500: le of reacting in sensitive radioim
Page 503 and 504: gic approach. In: Hiatt HH, Watson
Page 505 and 506: 12 Eco R [ Fig. 1. Hybridization pa
Page 509 and 510: 10 - '1~ )( -~ S:! E A U I o 1 2
Page 511 and 512: Haematology and Blood 'fransfusion
Page 513 and 514: cultures. These cells did not conta
Page 515 and 516: indication of malignant T-cells in
Page 517 and 518: Fig. 3. Thin-section electron micro
Page 519 and 520: Table 3. Lack of detectable related
Page 521 and 522: J. HTLV Was Present in the Primary
Page 523 and 524: specific signals into nonspecific s
Page 525 and 526: Table 2. Distribution of HTLV-relat
Page 527 and 528: u 10 ~ 20 ~ :c 30 a 40 o Fig. 1. Ki
Page 531 and 532: SSV TrS-gp labeled effectively with
Page 535 and 536: 60 HFF/SSV cDNA CELL RNAs: 0 HF/SSV
Page 537 and 538: Antisera a Table 1. Immunofluoresce
Page 539 and 540: Haematology and Blood 'ftansfusion
Page 541 and 542: y competition RIA yielded virtually
Page 543: SiS'V' gp70: ANTIGEN, ANTI BODY, IM
Page 547 and 548: c. Results The fractionated whole-b
Page 549 and 550: induced antibodies as well as exper
Page 551 and 552: - Fig. la-f. Retravirus particles p
Page 553 and 554: eplication of infecting murine leuk
Page 557 and 558: immunodeficiency, multic10nal lymph
Page 559 and 560: Bovine leukemia --, proteins of 499
Page 561 and 562: Human T- cell - -, characterization
Page 563 and 564: RNA -, of tumor virus 439 Rous sarc
Page 565 and 566: Haematology andBlood Transfusion Su
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