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Haematology and Blood Transfusion Vol. 26
Modern Trends in Human Leukemia IV
Edited by Neth, Gallo, Graf, Mannweiler, Winkler
© Springer-Verlag Berlin Heidelberg 1981
Lymphoproliferation and Heterotransplantation in Nude Mice: Tumor
Cells in Hodgkin's Disease*
V. Diehl, H. H. Kirchner, M. Schaadt, C. Fonatsch, and H. Stein
A. Summary
In the last 3 years we were able to establish
four long-term cultures from Hodgkin-derived
material [pleuraleffusions (2), bone marrow
(1), and peripheral blood (1)], consisting of
cells which represent morphologic and cytochemical
as well as cytogenetic features of their
in vivo ancestors. Two of these cell lines are
described in this paper.
These two lines share the same features:
non-B-T lymphocytes, non-macrophages, non
myeloid cells, EBV genome negative, monoclonality,
multiple numerical and structural
chromosome aberrations, and tumor formation
upon intracranial xenotransplantation in
nude mice.
The two remaining lines are being characterized
at the moment. The common characteristics
expressed synonymously in the two described
lines suggest that the Hodgkin tumor
cell does not seem to share the features of
marker-carrying lymphocytes, macrophages,
or myeloblasts. The cellular origin of these
cells is not dear. The loss of cellular differential
markers du ring the process of possible dedifferentiation
is discussed.
B. Introduction
The "Sternberg-Reed" (SR) and "Hodgkin"
(H) cells are considered to be the neoplastic
cells in Hodgkin's disease. Their cellular origin
* This work was supported by the Deutsche Forschungsgemeinschaft
Bad Godesberg (Di 184-
5/6)
is still subject of considerable controversy.
There are arguments for a histiocytic origin of
SR and H cells (Rappaport 1966; Mori and
Lennert 1969) as wen as for their lymphoid
origin (Dorfman et al. 1973; Papadimitriou et
al. 1978).
Because of the fragility of freshly isolated
Hand SR cells and the contamination with
numerous reactive cells the precise analysis of
their cell type is difficult. These limitations can
be overcome by the establishment of in vitro
proliferating cell populations with neoplastic
properties from patients with Hodgkin's disease.
The present report describes the features
of two established in vitro cell lines from the
pleural effusions of two patients with histologically
proven Hodgkin's disease.
c. Patients, Material, and Methods
The pleural effusions were obtained from a 37-yearold
woman (E.M.) with histologically proven Hodgkin's
disease, which was of the nodular sclerosing
type, stage IV B, and was primarily diagnosed in
1972 (L 428), and from a 36-year-old man (HR.)
with Hodgkin's disease, nodular sclerosing type,
which was diagnosed in 1976 from an inguinallymph
node biopsy (L 439).
I. Establishment of the In Vitro Cultures
The heparinized pleural effusion fluid was centrifuged
at 150 g for 10 min and the pellet was
resuspended in an 0.84%NH 4 Cl solution to desintegrate
the remaining erythrocytes. After two washings
cells were incubated in RPMI 1640 medium
supplemented with 20% fetal calf serum, glutamin,
and penicillin/streptomycin at 37° C in a 5 % CO 2 air
atmosphere.
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