Modern - Blog Science Connections

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Fig. 4. Stimulation of the rates of DNA synthesis in
contact-inhibited monolayers of primary chinese
hamster lung (CHL) cells and in monolayers of
mouse 3T3 cells after erythrocyte ghost media ted
microinjection of purified EBNA antigen. Rates of
DNA synthesis were followed by pulse labeling of
replicate slides with 1 uC;lml (3H)-thymidine for
2 h for each time point. Solid lines indicate cultures
microinjected with EBNA, broken lines cells microinjected
with a mock preparation (HMG proteins
from EBV-negative cells). (Zeuthen, unpublished
work)
CD
x = 59.3
x=50.4
o 20
40 60 80 100 120 140
o 20 40 60 80 100 120 140
+ TPA
,
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,
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• 1 11.1.1 • x = 85.6
i i i I i i i i i
,
40 60 80 100 120 140
+ TPA
x = 95.0
0 20 40 60 80 100 120 140
+ BUT
.• III.lhlil
I , i i i i i . i
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i
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+ BUT
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i i i , i i i , i i , I
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Fig. 5. Quantitative immunofluorimetric measurements of EBNA immunofluorescence stained (Reedman
and Klein 1973) slides prepared from control, TP A, and Na -butyrate induced cultures of DA UD I cells, 1 and
3 days after start of the experiment. In the induced cultures the average immunofluorescence values
approximately double; this effect was not observed with the abortive producer RAJI line (Zeuthen and
Ernberg, unpublished work). For discussion see text
186