Autologous Bone Marrow Transplantation - Blog Science Connections

Natural Killer Cells and Transplantation 677
Table 1. Induction of NK Cell Cytotoxic Activity in Bone Marrow of Normal
Donors by Interleukin 2
8
Donor Time in Culture
Percentage of Cytotoxicity6
Number (days) K-562 OV-2774
1 0 4.9 0.2
6 61.9 40.3
2 0 2.4 0.4
6 26.5 8.2
11 33.8 27.9
3 0 -0.7 -1.7
7 19.3 22.5
14 56.6 47.4
4 0 1.6 0.6
7 59.6 57.3
14 79.3 61.6
"Bone marrow mononuclear cells were cultured with 10 3 units of recombinant
interleukin 2.
"Cytotoxicity was tested at 1:12 target-to-effector cell ratio in a 3-hour ^Cr-release
assay as described previously (4).
Table 2. Induction of NK Cell Cytotoxic Activity Against K-562 in Bone Marrow
of Leukemic Patients
Type of
Leukemia
Time in Culture 3
(wks) Cytotoxicity" (%)
AML 0 1.7
1 4.1
2 18.9
CML 0 9.7
1 22.5
2 28.5
ALL 0 0.1
1 7.3
5 69.7
CLL 0 1.2
2 6.0
4 40.5
PRL 0 -0.4
1 48.9
4 71.0
"Bone marrow mononuclear cells were cultured with 10 3 units of recombinant
interleukin 2.
"Cytotoxicity was tested at target-to-effector cell ratios of 1:50 (AML), 1:25 (CML
and CLL), and 1:12 (ALL and
PRL).
Abbreviations: AML, acute myelogenous leukemia: CML, chronic myelogenous
leukemia; ALL, acute lymphoid leukemia; CLL, chronic lymphoid leukemia; PRL,
preleukemia.