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Autologous Bone Marrow Transplantation - Blog Science Connections

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452 Panel Discussion: Session III<br />

expensive, so I think that everybody who is doing marrow purging could<br />

actually have this in their laboratory.<br />

DR. FAVROT: I have one quick question regarding the TdT stain. So<br />

these cells are always stained on the cytospin slides or are they stained in<br />

suspension with this antibody?<br />

DR. REYNOLDS: The technique for simultaneous two-color staining<br />

involves staining of the viable cells in suspension for cell-surface antigens,<br />

with biotinylated J5, and then cytospinning the cells, fixing, and then staining<br />

with directly fluoresceinated anti-TdT, and at the same time adding<br />

phycoerythrin-avidin to develop the cell-surface antibody. But variations of<br />

that technique work as well.

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