Autologous Bone Marrow Transplantation - Blog Science Connections

440 Digital Image Analysis System
useable with minor modifications both on the new generation of faster
microcomputers that use the 80386 microprocessor (29) and with higher
speed graphics hardware based on the new generation of graphics chips (30).
Such increases in computer power should allow more and more automation
to be programmed into the system, resulting in a decrease in the time
required to analyze specimens. An increase in the speed of analysis will allow
the system to be modified to allow quantitation of antigen density on rare cells
of interest (31-34).
The system described here is already suitable for use in monitoring
purging experiments, using H342-marked cells as a model system (23), and
can be readily adapted to the analysis of immunostained clinical specimens.
Employing the appropriate immunostaining reagents, the system should find
use in other areas requiring rare-event analysis, including detection of
minimal leukemic disease in blood or marrow (34) or quantitating infrequent
normal cells such as hematopoietic progenitors in bone marrow.
ACKNOWLEDGMENT
This investigation was supported by Naval Medical Research and Development
Command Work unit MF58.527.007.0004. The opinions and
assertions contained herein are the private ones of the writers and are not to
be construed as official or reflecting the views of the Department of the Navy
or the naval service at large.
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