Autologous Bone Marrow Transplantation - Blog Science Connections

ABMTfor Neuroblastoma 381
PATIENTS
B3
BB7
12
• BEFORE PURGING
J MARRON SEDIMENTED
3 TO ENRICH FOR TUMOR
AFTER SEDIMENTATION
AND FILTRATION
I AFTER MAGNETIC
IIMMUNOBEAOS
BB6
]8
BB15
0 1
0
5.5
10 15 20
TUMOR CELLS/100. 000 MARROW CELLS
Figure 3. Removal of neuroblastoma cells from autologous marrow by sequential
sedimentation, filtration, and magnetic immunobead treatment. Marrow from four
patients (B3, BB7, BB6, and BB15) was treated with one cycle of immunobeads coated
with goat antimouse immunoglobulin and monoclonal antibodies 390,459, HSAN 1.2, and
BA-1. Tumor cells were identified in marrow by immunoperoxidase staining with
anti-cell-surface monoclonal antibodies (mixture of antibodies 390, 459, HSAN 1.2, and
126-4) and with anti-neuron-specific enolase serum.
diagnosed patients into the BMT phase by 20 weeks after diagnosis without
progressive disease and to emerge from the BMT phase with a 90% survival rate.
Because all patients are not expected to remain tumor-free following BMT,
efforts are being made to identify prognostic factors for this very aggressive
therapeutic approach; additional or different therapy will need to be developed
for those who are likely to develop progressive disease after BMT. Collectively,
these new strategies may further increase the percentage of patients who
survive tumor free.
ACKNOWLEDGMENTS
This study was supported in part by U.S. Public Health Service grants
CA12800, CA27678, and CA16042 from the National Cancer Institute, U.S.
Department of Health and Human Services; by a grant from Concern II; and by