Autologous Bone Marrow Transplantation - Blog Science Connections

Comparative Efficiency of an Immunomagnetic
Purging Procedure and a Rabbit Complement
Lysis for Eliminating Burkitt's Lymphoma Cells
From Bone Marrow
M. C. Favrot, 1. Philip, P. Poncelet, V. Combaret, B. Kremens,
G Janossy, and T. Philip
Several monoclonal antibodies that react with B lymphocytes are now available
for therapeutic use. We previously showed, according to the immunophenotype
of 28 Burkitt's lymphoma (BL) cell lines, that three of them, and Y29/55—
two pan B reagents—and AL 2
directed against CALLA (common acute lymphoblastoid
leukemia antigen), were expressed on BL cell lines, especially in
Caucasian ones (1 ). We used this cocktail of monoclonal antibodies to target
BL cells, either in a purging procedure using rabbit complement or in an
immunomagnetic depletion technique. A description of how to set up optimal
working conditions in the two techniques has been previously published (2,3;
see also Combaret et al. "Eliminating Burkitt's Cells From Excess Bone Marrow
With an Immunomagnetic Purging Procedure," in this volume). The aim of this
study was, first, to quantify the efficiency of two new IgM monoclonal antibodies,
RFB 7
(CD 20-like) and SB 4
(CD 19), in the rabbit complement lysis procedure
and, second, to compare in the same experiments the efficiency of both
procedures in optimal conditions. Such experiments were performed on three
cell lines freshly established from our patients' tumor cells, all three patients
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