Autologous Bone Marrow Transplantation - Blog Science Connections

180 Pharmacological Manipulation and Purging
Table 1. Effect of Verapamil on Uptake of 0.1 p.M Tritium-labeled Vincristine
by HL-60 Cells
Verapamil Cell-associated Vincristine (pmol/10 6 cells)
(uM) 15 min 2 hr 4 hr
0 3.4 5.6 6.9
2 3.9 6.1 6.9
5 3.9 7.1 8.0
10 6.1 7.8 9.6
20 6.9 10.6 10.3
40 9.1 11.4 12.0
50 11.8 13.5 13.6
Note: Actively proliferating HL-60/C1 cells (3 x 10 5 cells/ml) were combined with
equal amounts of growth medium containing tritium-labeled vincristine and verapamil
at twice the test concentrations. Cultures were incubated at 37° C, and at the times
indicated triplicate 1-ml samples were withdrawn and assayed for radioactivity as
described previously (21).
ioo-a-
3 io- o CFU-GM
* BFU-E
• CFU-GEMM
_i i i i i
10
100
VERAPAMIL, MM
Figure 1. Effects of verapamil alone (2.5-80 u.M, 4 hr, 37°C)on the formation of CFU-GM,
BFU-E, and CFU-GEMM colonies. Each symbol represents the mean value of eight
experiments.