Autologous Bone Marrow Transplantation - Blog Science Connections

Magnetic Separation of Marrow Cells 141
and MAC to eliminate K562 cells from mixtures (Fig 3). Starting with a mixture
of 1 K562 cell/10 bone marrow mononuclear cells, the clonogenic analysis
yielded a frequency of 1 in 14. After MAC separation, the frequency of K562
cells was 1 in 626,000. The analysis was still linear at this level of contamination.
With the recovery of 40%, this represents a 5-log removal of K562 cells.
Cens / Wei
s « 10*
After: 1/626.000
M
0»
Z 10-1
K562 + 40/60 CELLS SEPARATED WITH CF-1 • PM-81
-Before: 1/14
Figure 3. Limiting dilution clonal analysis of K562 cells mixed with irradiated peripheral
blood mononuclear cells before and after MAC separation with CF-1 and PM-81. The
frequency of K562 clonogenic cells in the mixture before separation is 1/14 cells. After
separation, the frequency is 1/626,000 cells. Considering the 40% cell recovery, this
represents a 5-log removal of K562 cells: log [(.40)(1/626,000)]/(1/14) = -5.05.