Autologous Bone Marrow Transplantation - Blog Science Connections

Magnetic Separation of Marrow Cells 135
Figure 1. As this schematic of the magnetic affinity colloid (MAC) procedure shows, bone
marrow (BM) cells are incubated with a cocktail of monoclonal antibodies (MCA), washed,
and incubated with biotinylatedgoat antimouse immunoglobulins (B GAM) andbiotinylated
soybean agglutinin. After washing, the cells are incubated with MAC, which binds to biotin
on the cells.
for CFCI-GM assays as previously described (6). Limiting dilution analysis of
granulocyte-macrophage precursors (LDA-GM), analysis of undifferentiated
blast (C1B) colonies, and colony assays of fresh acute leukemia cells are
performed as described elsewhere (7-9).
The monoclonal antibodies OKT-3, OKT-4, OKT-8, OKT-11, and L-243 were
purified from cultures of mycoplasma-free hybridomas (American Type
Culture Collection, Rockville, MD). The CF-1 hybridoma line was a gift from
Centocor (Malvern, PA). The CLB-gran mon/2, from cluster of differentiation