Madina Rasulova Molecular Systematics of Nematodes Page 1

phylogenetic trees based on the posterior probability distribution of trees which resulted in files including
“PROJECT_BItree.mcmc”, “PROJECT_BItree.con” and several others. Finally, all obtained phylogenetic
trees were visualized by the help of the program TreeView.
RESULTS
The study of the given nucleotide sequence of Belonolaimus genus by BLAST in NCBI GeneBank
revealed partial sequence of 18S ribosomal RNA of Belonolaimus longicaudatus GV-2 (accession number
DQ494797) with complete internal transcribed spacer 1 (ITS1) region and partial 5.8S ribosomal RNA
gene the authors of which were Han, Jeyaprakash, Weingarther and Dickson (2006).
4 isolates from 3 species of the Belonolaimus genus and 6 isolates of B.longicaudatus with different
rates of similarities in ITS region were studied and used in phylogenetic analysis as in-group (indicated in
pink colour). Such species as Ditylenchus dipsaci (Tylenchina: Anguinidae), Hoplolaimus columbus
(Tylenchina: Hoplolaimidae) and Tylenchorynchus annulatus (Tylenchina: Belonolaimidae) were chosen
as out-group (indicated in blue colour). Despite the fact that all nematodes mentioned above are from the
same suborder Tylenchina, the latter three species belong to other families (except the last species) and
genera, which makes it possible to use them as out-group (Table 3).
Such alignment characteristics as the total length of studied sequences as well as variations in length
are given below (Table 3). According to the data, the total length and variation of the specimen of our
choice is the same, 685 bp in fact.
Table 3. The list of species used for the project work.
№ Accession Species E value Max identity
Total
Length
(bp)
Variation
(bp)
1 DQ494797 Belonolaimus longicaudatus 0.0 100 685 685
Madina Rasulova Molecular Systematics of Nematodes Page 8