Madina Rasulova Molecular Systematics of Nematodes Page 1

More documents

Recommendations

Info

longicaudatus DQ672373 is very similar to the one we are studying in the current work (DQ494797) and that is the reason why they both occur in the same clade in all phylogenetic trees (discussed below). Figure 2. Distance matrix. Trees obtained from 4 phylogenetic methods of analysis have a bit different topology except MP and ML cases. According to NJ-phylogram (Figure 7, 8) the basal clade (since it is unresolved situation) consists of 2 branches, one is EF030983, while the other one divides into GQ469496 and DQ309584. The latter basal clade consists of only out-group species and is highly supported, 100% in fact. The in-group species are divided into 2 clades: one of which is highly supported (100%) and composed of DQ672385, DQ672382 and DQ494803. Despite the fact that the second clade is supported only by 77%, it includes all B.longicaudatus and only one B.gracilis (DQ672386) with BS=98%. The position of studied sequence sample is in one branch with DQ672373. This clade and its sister clade as well as the branch forming these two clades are all supported by 100%. In MP-cladogram (Figure 7, 9) the basal clades are similar as in NJ-phylogram and one of them is supported by 100%. Then it forms one clade with only one DQ672386 which in its turn divides into two clades: the first one consisting of only B.longicaudatus species (BS=88%) and the second one comprised Madina Rasulova Molecular Systematics of Nematodes Page 10
of other species of the same genus (BS=100%) as well as only one B.longicaudatus DQ672384. If considering the first sister clade, it is very well supported: it has one branch supported by 98% which divides into two clades both of which are supported by 100%. The position of the studied B.longicaudatus is exactly the same as in NJ-phylogram. The phylogenetic tree of ML (Figure 7, 10) is exactly the same as of MP with only differences in the percentage by which each branch is supported. The highest BS percentage occurs only in one case: in one of the basal clades (BS=100%). Relatively higher BS is where the clade forms the branch of the studied B.longicaudatus DQ494797 and another one DQ672373, 91% in fact. The supported rate of other branches is very low. The BI-phylogram (Figure 11) depicts that almost all branches are highly supported: one of the basal clades with consisting of Ditylenchus dispaci and Hoplolaimus columbus; in in-group: the clade comprised of species of the genus Belonolaimus except B.longicaudatus; the branch with Belonolaimus sp.; and two small branches consisting only out of B.longicaudatus including the studied one are all supported by 100%. The clade constituted of these two small branches is also well supported, 98% in fact. The position of out-groups and the studied B.longicaudatus remains the same as in other phylogenetic trees. Though NJ, MP and BI phylograms (Figures 7-11) are not the same by their topology, they have some clades which remain unchangeable in all types of trees which will be described below. Since the relationships in the out-groups are not completely resolved, it is complicated to indicate which species or which branch is a basal clade and, thus, all three species are considered as basal clades. Another similarity is found in the clade consisting of only B.longicaudatus species as well as the position of the studied one is constant as well. Besides, other members of the genus Belonolaimus are also always gathered in one clade in all 4 trees. The position of other inside clades is rather changeable. Surprisingly, the only one Madina Rasulova Molecular Systematics of Nematodes Page 11
Page 1 and 2: Madina Rasulova Molecular Systemati
Page 3 and 4: very sandy soils and are active whe
Page 5 and 6: level analyses because of appreciab
Page 7 and 8: constraining, rooting and testing h
Page 9: 2 DQ672373 Belonolaimus longicaudat
Page 13 and 14: ermudagrass (Poteet) isolates were
Page 15 and 16: outside of Florida are reproductive
Page 17 and 18: Han H-R., Jeyaprakash A., Weingarth
Page 19 and 20: APPENDIX Figure 4. Results of BLAST
Page 21 and 22: Figure 7. NJ, MP and ML trees with
Page 23 and 24: Figure 9. Phylogenetic tree MP. Max
Page 25: Figure 11. Phylogenetic tree MrB. B

Madina Rasulova Molecular Systematics of Nematodes Page 1

Create successful ePaper yourself

Delete template?

Save as template?