S. Álvarez-Ortega et al. Fig. 5. Metaporcelaimus ovogranulosus sp. n. (female). A, B: Anterior region in median view; C: Lip region in surface, lateral view; D: Pharyngeal expansion; E, J: Vagina; F: Pharyngo-intestinal junction; G, N; Uterus <strong>and</strong> vagina, arrow head pointing at oviduct-uterus junction; H, I: Caudal region; K-M; Uterine egg, showing granules on shell; O: Entire body. (Scale bars: A, B, H, I = 10 μm; C, E, J = 5 μm; D = 100 μm; F, G, K-N = 20 μm; O = 500 μm.) 10 Nematology
Studies on Metaporcelaimus Table 2. Morphometric data <strong>of</strong> female Metaporcelaimus ovogranulosus sp. n. All measurements are in μm except L in mm, <strong>and</strong> in the form: mean ± s.d. (range). Character El Dorado County Salix sp. <strong>and</strong> other trees Holotype Paratypes n – 18 L 1.96 1.75 ± 0.12 (1.54-1.96) a 40 36.2 ± 1.5 (34-39) b 3.6 3.9± 0.2 (3.6-4.2) c 49 43.7 ± 3.5 (39-52) c ′ 1.5 1.5 ± 0.1 (1.4-1.6) V 51 49.9 ± 0.9 (48-51) Lip region diam. 19 18.2 ± 0.6 (17-19) Odontostyle length 18 18.6 ± 0.4 (17-19) Odontophore length 34 32.9 ± 1.7 (27-35) Guiding ring from ant. end 8.5 8.7 ± 0.3 (8.0-9.5) Neck length 539 448 ± 23.0 (415-480) Pharyngeal expansion length 275 230 ± 13.8 (213-253) Diam. at neck base 47 45.6 ± 3.7 (38-53) at mid-body 49 49.0 ± 3.6 (42-56) at anus 28 26.9 ± 1.6 (24-30) Prerectum length 79 83.3 ± 32.4 (53-161) Rectum length 35 32.4 ± 2.2 (27-35) Tail length 40 40.4 ± 2.6 (35-44) occasionally visible but poorly developed. Lip region <strong>of</strong>fset by deep constriction, 2.8-3.1 times as broad as high <strong>and</strong> one-third to one-half (35-46%) <strong>of</strong> body diam. at neck base, lips angular <strong>and</strong> moderately separated, labial papillae slightly protruding. Amphid fovea funnel-shaped, its aperture 9.0-9.5 μmorca one-half (47-54%) <strong>of</strong> lip region diam. Cheilostom nearly cylindrical, lacking any differentiation. Odontostyle typical <strong>of</strong> genus, 4.9-5.4 times as long as wide, 0.9-1.0 times as long as lip region diam., <strong>and</strong> 0.9-1.14% <strong>of</strong> body length, aperture 11-14 μm long or occupying up to three-fourths (70-74%) its length. Guiding ring plicate. Odontophore linear, rod-like, 1.5-1.9 odontostyle lengths long. Anterior region <strong>of</strong> pharynx enlarging very gradually; basal expansion 6.7-9.1 times as long as wide, 4.7-5.8 times as long as body diam., occupying 49-53% <strong>of</strong> total neck length, pharyngeal gl<strong>and</strong> nuclei apparently located as follows: DN = 58-61, DO-DN = 21 μm (n= 1), S 1 N 1 = 64% (n = 1), S 1 N 2 = 71-72% (n = 3), S 2 N = 86-89% (n = 3). Nerve ring located at 136-161 μm from anterior end or 30-34% <strong>of</strong> total neck length. Cardia conical, (14-17) × (10-14) μm, its junction with pharyngeal base surrounded by weak ring-like structure, visibly asymmetrical as dorsal surface is larger than ventral, dorsal cellular mass present at level <strong>of</strong> anterior end <strong>of</strong> intestine in some specimens. Genital system didelphic-amphidelphic, with both branches equally <strong>and</strong> moderately developed, anterior 118-229 μm or7- 13% <strong>of</strong> body length (178-261 μm long or 11-14% <strong>of</strong> body length with an egg inside), <strong>and</strong> posterior 113-190 μm or 7-11% <strong>of</strong> body length (178-253 μm long or 10-13% <strong>of</strong> body length with an egg inside). Ovaries comparatively large, anterior 64-204 μm <strong>and</strong> posterior 69-239 μm long, oocytes arranged first in two or more rows, then in a single row. Oviduct 66-119 μm long or 1.4-2.3 times corresponding body diam., consisting <strong>of</strong> slender part with prismatic cells <strong>and</strong> a very poorly developed pars dilatata with very narrow lumen. Oviduct-uterus junction barely perceptible. Uterus a simple tube 27-41 μm long or 0.6-0.8 times corresponding body diam. Uterine eggs ovoid, (88- 103) × (33-37) μm, 2.4-2.8 times as long as wide, its shell bearing small but abundant granular bodies. Vagina extending inwards 19-24 μm, occupying two-fifths to onehalf (38-52%) <strong>of</strong> body diam., pars proximalis 11-17 × 13-19 μm, with somewhat sigmoid walls <strong>and</strong> surrounded by weak musculature, pars refringens with two adjacent triangular to rounded pieces measuring (5.5-7.5) × (4- 5) μm, combined width <strong>of</strong> 7.5-10.5 μm, pars distalis 1.5- 2.5 μm long. Vulva a nearly equatorial, transverse slit. Prerectum 2.0-3.6, rectum 1.0-1.5 anal body diam. long. Tail conical with rounded tip, ventrally straight, dorsally convex or with a weak concavity at terminus, inner core somewhat sunken at both sides <strong>and</strong> forming a digitate, terminal extension nearly reaching tail tip, hence hyaline portion <strong>of</strong> tail is very short. Two pairs <strong>of</strong> caudal pores, subdorsal, at middle <strong>of</strong> tail. Male Unknown. TYPE LOCALITY AND HABITAT American River Parkway, River Bend Park, El Dorado County, California, USA, where the new <strong>species</strong> was collected in association to willow (Salix sp.) <strong>and</strong> other trees. TYPE MATERIAL Female holotype <strong>and</strong> 16 female paratypes deposited in the nematode collection <strong>of</strong> the University <strong>of</strong> Jaén, Spain. Two female paratypes deposited with USDA Nematode Collection, Beltsville, MA, USA. Vol. 00(0), 2012 11
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