Chemical Hygiene Plan - Queensborough Community College ...

22.8 Autoclaves
Autoclaves have the following potential hazards:
• Heat, steam, and pressure.
• Thermal burns from steam and hot liquids.
• Cuts from exploding glass.
Some general safety guidelines to follow when using autoclaves:
• All users should be given training in proper operating procedures for using the autoclave.
• Read the owner’s manual before using the autoclave for the first time.
• Operating instructions should be posted near the autoclave.
• Follow the manufacturer’s directions for loading the autoclave.
• Be sure to close and latch the autoclave door.
• Some kinds of bottles containing liquids can crack in the autoclave, or when they are
removed from the autoclave. Use a tray to provide secondary containment in case of a
spill, and add a little water to the tray to ensure even heating.
• Only fill bottles half way to allow for liquid expansion and loosen screw caps on bottles
and tubes of liquid before autoclaving, to prevent them from shattering.
• Do not overload the autoclave compartment and allow for enough space between items
for the steam to circulate.
• Be aware that liquids, especially in large quantities, can be superheated when the
autoclave is opened. Jarring them may cause sudden boiling, and result in burns.
• At the end of the run, open the autoclave slowly: first open the door only a crack to let
any steam escape slowly for several minutes, and then open all the way. Opening the
door suddenly can scald a bare hand, arm, or face.
• Wait at least five minutes after opening the door before removing items.
• Large flasks or bottles of liquid removed immediately from the autoclave can cause
serious burns by scalding if they break in your hands. Immediately transfer hot items
with liquid to a cart; never carry in your hands.
• Wear appropriate PPE, including eye protection and insulating heat-resistant gloves.
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