Cell Culture

Cell Culture 

2012

Cell Culture 

www.promocell.com 

Cell Biology 

www.promokine.info 

Training Courses 

www.promocell-academy.com 

Please visit our websites regularly for upcoming new products and training courses

Overview 1 

Overview 

1 

Human Primary Cells 

2 

Human Stem and Blood Cells 

3 

Cell Model Systems 

4 

Media and Sera 

5 

Cell Pellets 

6 

Reagents and Supplements 

7 

Microbial Detection and Elimination 

8 

PromoKine Cell Biology Products

2 

Contents 

Contents 

About PromoCell 

PromoCell Ethical Standards ......................................... 4 

Introduction. ..................................................... 5 

1 


1.1 Cardiac Myocytes . ............................................ 9 

1.2 Chondrocytes ............................................... 10 

1.3 Endothelial Cells (Large Vessels) ................................. 11 

1.4 Endothelial Cells (Microvascular) new cell ............................. types 

15 

1.5 Epithelial Cells ............................................... 21 

1.6 Fibroblasts. ................................................. 25 

1.7 Follicle Dermal Papilla Cells .................................... 28 

1.8 Hepatocytes ................................................ 29 

1.9 Keratinocytes new cell ............................................ types 

30 

1.10 Melanocytes . ............................................... 31 

1.11 Osteoblasts ................................................ 32 

1.12 Preadipocytes . .............................................. 33 

1.13 Skeletal Muscle Cells ......................................... 34 

1.14 Smooth Muscle Cells ......................................... 35 

Overview Human Primary Cells ...................................... 40 

Human Primary Cells by Tissue Type .................................. 43 

2 


2.1 Mesenchymal Stem Cells ...................................... 47 

2.2 Pericytes ................................................... 49 

2.3 CD34 + Progenitor Cells ........................................ 50 

2.4 CD133 + Progenitor Cells ....................................... 51 

2.5 Monocytes new .............................................. cell types 

52 

2.6 Mononuclear Cells new cell ........................................ types 

54 

Overview Human Stem and Blood Cells. ............................... 56 

3 

4 new 


3.1 Angiogenesis Assays and Kits ................................... 59 


4.1 Media for Primary Cells cell .................................... types 

67 

4.2 Media for Stem and Blood Cells new cell .............................. types 

86 

4.3 Classical Media .............................................. 90 

4.4 Insect Cell Culture Media. ...................................... 92 

4.5 Sera. ...................................................... 92

Contents 3 

5 

6 new 


5.1 Cell Pellets new cell .............................................. types 

97 


6.1 Detachment Reagents cell .................................... types 

101 

6.2 Antibiotics and Antimycotics ................................... 103 

6.3 Buffers and Salt Solutions ..................................... 104 

6.4 Supplements new cell ........................................... types 

105 

6.5 Cytokines and Growth Factors ................................. 106 

7 

Microbial Detection and Elimination 

7.1 Mycoplasma Test Kits ........................................ 109 

7.2 Mycoplasma Elimination Solutions. .............................. 110 

7.3 Bacteria Test Kit. ............................................ 112 

7.4 Disinfectants ............................................... 113 

8 

PromoKine Cell Biology Products 

8.1 Cell Analysis ............................................... 117 

8.2 Apoptosis ................................................. 117 

8.3 Fluorescent Labeling ......................................... 117 

8.4 Gene Cloning, Expression, and Silencing .......................... 118 

8.5 Cell Transfection ............................................ 118 

8.6 Antibodies and ELISAs. ....................................... 118 

8.7 Cytokines and Growth Factors ................................. 119 

9 

Appendix 

Alphabetical Index .............................................. 120 

Numerical Index ................................................ 124 

International Distributors .......................................... 131 

Terms and Conditions ............................................ 134 

Ordering and Technical Support. .................................... 136 

Fax Order Form ................................................. 137

4 About PromoCell 

PromoCell Ethical Standards 

Ethical and legal standards 

governing Primary Human Cells 

PromoCell is the original manufacturer 

of all primary, stem, and blood cells featured 

in this catalog and is committed to 

the highest ethical and legal standards. 

Consequently, PromoCell strictly complies 

with the following procedure for 

the donation and collection of human 

tissue used for cell isolation: 

The tissue used by PromoCell for the 

isolation of human cell cultures is derived 

from donors who have signed 

an informed consent form (this being 

done by the donor himself, an authorized 

agent, or a legal agent) which 

outlines in detail the purpose of the 

donation and the procedure for processing 

the tissue. The tissue used by 

PromoCell is collected exclusively in 

Germany. 

Extract from “The Convention of Human Rights and Biomedicine”: 

When obtaining and using human 

tissue, PromoCell acts in strict compliance 

with 

Article 5 – General rule 

An intervention in the health field may only be carried out after the person 

concerned has given free and informed consent to it. 

This person shall beforehand be given appropriate information as to the purpose 

and nature of the intervention as well as on its consequences and risks. 

The person concerned may freely withdraw consent at any time. 

Article 22 – Disposal of a removed part of the human body 

When in the course of an intervention any part of a human body is removed, 

it may be stored and used for a purpose other than that for which it was 

removed, only if this is done in conformity with appropriate information and 

consent procedures. 

Extract from “The Declaration of Helsinki”: 

Article 22 

In any research on human beings, each potential subject must be adequately 

informed of the aims, methods, sources of funding, any possible conflicts 

of interest, institutional affiliations of the researcher, the anticipated benefits 

and potential risks of the study and the discomfort it may entail. The subject 

should be informed of the right to abstain from participation in the study or 

to withdraw consent to participate at any time without reprisal. After ensuring 

that the subject has understood the information, the physician should then 

obtain the subject‘s freely-given informed consent, preferably in writing. If 

the consent cannot be obtained in writing, the non-written consent must be 

formally documented and witnessed. 

1. “The Convention for Protection 

of Human Rights and Dignity of the 

Human Being with Regard to the 

Application of Biology and Medicine: 

Convention of Human Rights and 

Biomedicine” published on April 4 th , 

1997 by the Council of Europe (European 

Treaty Series – no 164). 

2. “The Human Tissue Act” published 

on November 15 th , 2004 by the 

government of the United Kingdom. 

This act aims to make consent a fundamental 

principle underpinning the 

use and storage of human tissue. 

3. “The Declaration of Helsinki” developed 

by the World Medical Association 

as a statement of ethical principles 

to provide guidance to physicians and 

other participants in medical research 

involving human subjects. 

Should you have any questions concerning 

ethical aspects related to the 

use of Primary Human Cells, do not 

hesitate to contact us or visit our 

website: 

www.promocell.com/ethics

About PromoCell 

5 

The PromoCell Customer Care Team 

Dear Customer, 

For more than 20 years PromoCell has been your premier manufacturer 

of primary cell culture products, with a broad range of human primary 

cells, stem cells, and blood cells as well as optimized cell culture media. 

We are proud that scientists worldwide use PromoCell products in basic 

and applied biomedical research to obtain better results from more accurate 

physiological models. 

With our new catalog we are happy to offer an extended stem and blood 

cell product range now covering Dendritic Cell Research with a comprehensive 

media portfolio and CD14 + monocytes for several applications. 

In addition, we have developed a xeno-free cell culture system for mesenchymal 

stem cells facilitating chemically defined culture conditions. 

In chapter 8 we would like to present the PromoKine brand, which offers 

a wide range of well proven products for cell biology research, such as 

cell analysis kits, cell transfection reagents, fluorescent dyes, antibodies, 

ELISAs and cytokines. 

As scientists, we understand the needs of fellow scientists, our customers. 

This feeling is the stimulus for how we act: Our mission, is to help 

you obtain optimal results in your daily labwork. 

Scientists supporting Scientists – guaranteed. 

Your PromoCell Team


1 

Experiments with human primary cells simulate in vivo conditions 

much better and yield more significant results than those 

using immortalized cell lines. This is because the primary cells’ 

genome and expression patterns have not been altered by 

immortalization and numerous passages in culture. 

PromoCell offers a wide variety of high quality human primary 

cells from tissue obtained from approved medical centers.

8 

Content Chapter 1 

1 


1.1 Cardiac Myocytes .................... 9 

1.2 Chondrocytes ...................... 10 

1.3 Endothelial Cells (Large Vessels) ........ 11 

Umbilical Vein ...................... 11 

Umbilical Artery. .................... 12 

Aortic / Coronary Artery .............. 13 

Pulmonary Artery / Saphenous Vein ..... 14 

1.4 Endothelial Cells (Microvascular) ........ 15 

Dermal ........................... 15 

Dermal Blood ...................... 16 

Dermal Lymphatic ................... 17 

Bladder / Cardiac. ................... 18 

Pulmonary / Uterine ................. 19 

Brain new ........................... 20 

1.5 Epithelial Cells ...................... 21 

Nasal / Tracheal. .................... 21 

Bronchial / Small Airway .............. 22 

Mammary / Renal ................... 23 

Renal Cortical / Placental. ............. 24 

1.6 Fibroblasts. ........................ 25 

Dermal ........................... 25 

Pulmonary / Aortic Adventitial / Cardiac .. 26 

Uterine / Villous Mesenchymal ......... 27 

1.7 Follicle Dermal Papilla Cells ............ 28 

1.8 Hepatocytes ....................... 29 

1.9 Keratinocytes new .................... 30 

1.10 Melanocytes ....................... 31 

1.11 Osteoblasts ........................ 32 

1.12 Preadipocytes ...................... 33 

1.13 Skeletal Muscle Cells ................. 34 

1.14 Smooth Muscle Cells ................. 35 

Aortic ............................ 35 

Coronary Artery / Pulmonary Artery ..... 36 

Umbilical Artery / Tracheal ............ 37 

Bronchial / Bladder .................. 38 

Prostate / Uterine ................... 39 

Overview Human Primary Cells .............. 40 

Human Primary Cells by Tissue Type .......... 43

1 Human Primary Cells 9 


PromoCell offers a wide variety of high 

quality human primary cells. The tissue 

used for the isolation of these cells is 

obtained from approved medical centers 

following strict ethical standards 

(see page 4 for details). Shortly after 

isolation, the cells are cryopreserved 

using the unique serum-free PromoCell 

Cryo-SFM (see page 85) and an optimized, 

computer controlled freezing 

profile. This allows high viability of the 

cells and defined culture conditions after 

thawing. 

All PromoCell primary cells are available 

cryopreserved or proliferating. Each cell 

lot is tested for cell type specific markers, 

cell morphology, population doubling 

time, and proliferation capacity. In addition, 

tests for the absence of HIV 1, HIV 2, 

HBV, HCV, fungi, mycoplasma, and other 

bacteria are performed. 

Moreover, cell pellets prepared from released 

cell lots and stored in RNAlater ® 

are available. They are useful for DNA, 

RNA, and protein isolation and their subsequent 

analysis (for more information 

see page 97). 

1 Human Primary 

Cells 

1.1 Cardiac Myocytes 

Applications 

Cardiac research 

Physiological studies 

Pharmacological studies 

Features 

Adult origin 

Sarcomeric α-actinin positive 

Slow muscle myosin positive 

Qualified for long-term experiments 

Human Cardiac Myocytes (HCM) are 

isolated from the ventricles of the adult 

heart. They are qualified for in vitro 

research on cardiac diseases and for 

pharmacological studies. Unlike freshly 

isolated rod-shaped myocytes, cultured 

HCM can be used for long-term experiments 

like investigating the long-term 

effects of cytokines, mechanical strain, 

or cell-cell interactions, as they are prepared 

according to a special protocol. 

Initially, the HCM act more like progenitor 

cells in that they are not yet fully differentiated. 

They express the markers 

of early stage differentiation such as 

GATA-4 and sarcomeric alpha-actin and 

have a high capacity for proliferation. 

When they are grown to confluency 

and cultivated for an extended period of 

time, the differentiation process begins. 

Markers of late differentiation (e.g. sarcomeric 

alpha-actinin, slow muscle myosin) 

are increased and the cells begin to 

form myotube-like structures. 

Recommended media & reagents 

Myocyte Growth Medium (see page 68) 

DetachKit (see page 101) 

Cryo-SFM (see page 85) 

Fig. 1: HCM culture in phase contrast. 

Related products 

Human Cardiac Microvascular 

Endothelial Cells (see page 18) 

Human Cardiac Fibroblasts 

(see page 26) 

HCM Pellet (see page 97) 

PromoFectin Transfection Reagent 

(see PromoKine catalog) 

Count 

75 Sarcomeric 

Sarcomeric 

α-actinin negative α-actinin positive 

Fig. 2: Flow cytometric analysis of PromoCell HCM. 

96 % of the cells are sarcomeric α-actinin positive. 

50 

25 

0 

0 

10 10 2 10 3 10 4 

Cell Lab Quanta SC TM , Beckman Coulter, Inc. 

Sarcomeric α-actinin 

https://www.promocell.com/shop 

Product Size Catalog Number 

Human Cardiac Myocytes (HCM) 

500,000 cryopreserved cells 

500,000 proliferating cells 

C-12810 

C-12811 

HCM Pellet 1x10 6 cells in RNAlater ® C-14080

10 

1 Human Primary Cells 

1.2 Chondrocytes 

Applications 

produce and maintain the extracellular 


matrix of cartilage, i.e. collagen (mostly 

Chondrocyte Growth Medium 

Osteoarthritis research 

type II) and proteoglycans (primarily ag- 

(see page 68) 



Joint research 

Physiology studies 

Chondrocyte differentiation 

experiments 

Hyaline cartilage investigations 

grecan). 




HCH Pellet (see page 97) 


Features 


Available from hip and knee joints 

Differentiation tested by Alcian Blue 

staining of HCH spheroids 

Fig. 1: HCH culture in phase contrast. 

Cryopreserved at second passage 

Human Chondrocytes (HCH) are isolated 

from normal human articular cartilage 

from the knee and hip joints (lot specific 

source information is available on request). 

PromoCell HCH are routinely 

tested for their capacity to differentiate 

in 3D spheroids (see Fig. 2). 

The articular cartilage covers the joints 

between bones and contains no blood 

vessels, lymphatic vessels, or nerve fi- 

Fig. 2: Spheroids of differentiated HCH 

bers. It is composed of only one special- 

cultured in Chondrocyte Growth Medium 

ized cell type, the chondrocytes, which 

and stained with Alcian Blue. 



Human Chondrocytes (HCH) 



C-12710 

C-12750 

HCH Pellet 1x10 6 cells in RNAlater ® C-14070


1.3 Endothelial Cells (Large Vessels) 

Applications 

Angiogenesis research 

Atherosclerosis research 

Oncology 

Drug uptake studies 

Cell-cell junction investigation 

Inflammation studies 

Features 

Tunica externa 

(Connective tissue) 



Von Willebrand factor (vWF) positive 

CD31 positive 

Smooth muscle α-actin negative 

Tunica media 

(Smooth muscle cells) 

The endothelium is the thin layer of endothelial 

cells that line the interior surface 

of blood and lymphatic vessels - from the 

aorta to the smallest capillaries. In large 

vessels (arteries and veins), it is enclosed 

by two other layers namely the tunica media 

and the tunica externa (see Fig. 1). 

PromoCell endothelial cells (large vessels) 

are available from the umbilical 

vein and artery, the aorta, the coronary 

artery, the pulmonary artery, and the 

saphenous vein. All endothelial cells are 

tested for positive expression of vWF and 

CD31, and the absence of smooth muscle 

α-actin. 

Available endothelial cell types (large vessels): 

HUVEC (see page 11) 

HUAEC (see page 12) 

HAoEC (see page 13) 

HCAEC (see page 13) 

HPAEC (see page 14) 

HSaVEC (see page 14) 

Tunica intima 

(Endothelial cells) 

Fig. 1: Cross section of an artery with the predominant cell types. 

Fig. 2: HUVEC culture stained for vWF Fig. 3: HUVEC culture stained for CD31 

(Factor VIII antigen). Nuclei counterstained 

with DAPI. 

antigen. Nuclei counterstained with 

DAPI. 

Human Umbilical Vein Endothelial 

Cells (HUVEC) 

HUVEC are isolated from the vein of the 

umbilical cord and are commonly used 

for physiological and pharmacological 

investigations, such as macromolecule 

transport, blood coagulation, angiogenesis, 

and fibrinolysis. 

PromoCell offers HUVEC from single 

donors or pooled from up to four 

different donors*. 

In addition, HUVEC are available which 

are pre-screened for angiogenesis and 

other VEGF (vascular endothelial growth 

factor) dependent signaling studies. 

They have been positively tested for 

VEGF response using PromoCell´s unique 

3D-Angiogenesis Assay (see page 59). 

VEGF is an important signaling protein 

involved in both vasculogenesis 

and angiogenesis. In vitro, VEGF has 

been shown to stimulate endothelial 

cell mitogenesis, cell migration, sprouting, 

and microvascular permeability. 


Endothelial Cell Growth Medium 

(see page 69) 

Endothelial Cell Growth Medium 2 

(see page 69) 




HUVEC Pellet (see page 98) 

HUVEC pooled Pellet (see page 98) 

HUVEC pre-screened Pellet 

(see page 98) 

Human Umbilical Artery Endothelial 

Cells (see page 12) 

Angiogenesis Assays and Kits 

(see page 59-63) 

PromoFectin-HUVEC Transfection 

Reagent (see PromoKine catalog) 

VEGF (see page 106) 

CD31 and VEGF Antibodies 

(see www.promokine.info) 

* Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.

12 




50 µm 50 µm 

Fig. 4: HUVEC culture in phase contrast. 

Fig. 5: Unstimulated (left) and VEGF (20 ng/ml) stimulated HUVEC-spheroids (right). 

See also 3D-Angiogenesis Assay, page 59. 



Human Umbilical Vein Endothelial Cells 

(HUVEC) single donor 



C-12200 

C-12250 

HUVEC Pellet 1x10 6 cells in RNAlater ® C-14010 


(HUVEC) pooled 



C-12203 

C-12253 

HUVEC pooled Pellet 1x10 6 cells in RNAlater ® C-14011 


(HUVEC) pre-screened 



C-12205 

C-12255 

HUVEC pre-screened Pellet 1x10 6 cells in RNAlater ® C-14012 

Human Umbilical Artery 

Endothelial Cells (HUAEC) 

HUAEC are isolated from the arteries of 

the umbilical cord and are often used for 

in vitro studies on atherosclerosis but 

also for endothelial cell-dependent lymphocyte 

activation and chemoattractant 

activity experiments * . 



(see page 69) 


(see page 69) 




HUAEC Pellet (see page 98) 



Human Umbilical Artery Smooth 

Muscle Cells (see page 37) 



CD31 Antibody 




Human Umbilical Artery Endothelial Cells 

(HUAEC) 



C-12202 

C-12252 

HUAEC Pellet 1x10 6 cells in RNAlater ® C-14013 

*Human Umbilical Vein Endothelial Cells (HUVEC) from the same donor are available on request.


Human Aortic Endothelial Cells 

(HAoEC) 


Endothelial Cell Growth Medium MV 


HAoEC Pellet (see page 97) 

(see page 70) 

Human Aortic Smooth Muscle Cells 

HAoEC are isolated from the human 

Endothelial Cell Growth Medium MV2 

(see page 35) 

ascending (thoracic) and descending 

(abdominal) aorta. They are useful 

for studying vascular diseases such as 

thrombosis, atherosclerosis, and hypertension 

as well as for stent-graft compat- 

(see page 70) 



Human Aortic Adventitial Fibroblasts 

(see page 26) 



CD31 Antibody 



ibility testing. 




Human Aortic Endothelial Cells (HAoEC) 



C-12271 

C-12272 

HAoEC Pellet 1x10 6 cells in RNAlater ® C-14023 

Human Coronary Artery 

Endothelial Cells (HCAEC) 

HCAEC are isolated from the coronary 

arteries (the right and the left coronary 

artery, including the anterior descending 

and the circumflex branches) from a 

single donor. They are crucially involved 

in the regulation of coronary blood flow 

and cardiac functions and are consequently 

useful for in vitro studies of 

diseases such as thrombosis, atherosclerosis, 

and hypertension. HCAEC are also 

suitable for studying functional vasodilators, 

such as prostacyclin, bradykinin, 

or nitric oxide. 



(see page 70) 


(see page 70) 



Left main coronary 

artery 

Circumflex coronary 

artery 


HCAEC Pellet (see page 97) 

Human Coronary Artery Smooth 




CD31 Antibody 


Right coronary artery 

Fig. 6: Location of the coronary arteries. 

Left anterior descending 

coronary artery 



Human Coronary Artery Endothelial Cells 

(HCAEC) 



C-12221 

C-12222 

HCAEC Pellet 1x10 6 cells in RNAlater ® C-14022

14 


Human Pulmonary Artery 

Endothelial Cells (HPAEC) 




HPAEC Pellet (see page 98) 

(see page 69) 

Human Pulmonary Artery Smooth 



HPAEC are isolated from human pulmonary 

arteries (the main pulmonary 

artery, left and right branches). Since the 

pulmonary arteries carry blood from the 

heart to the lungs, they are the only ar- 


(see page 69) 






CD31 Antibody 


teries (other than umbilical arteries) that 

carry deoxygenated blood. 



Human Pulmonary Artery Endothelial Cells 

(HPAEC) 



C-12241 

C-12242 

HPAEC Pellet 1x10 6 cells in RNAlater ® C-14024 

Human Saphenous Vein 

Endothelial Cells (HSaVEC) 

HSaVEC are isolated from human saphenous 

veins from single donors. The 

saphenous vein is often used for autotransplantation 

in coronary artery bypass 

operations, when arterial grafts are not 

available or many grafts are required. 



(see page 69) 


(see page 69) 




HSaVEC Pellet (see page 98) 



CD31 Antibody 




Human Saphenous Vein Endothelial Cells 

(HSaVEC) 



C-12231 

C-12232 

HSaVEC Pellet 1x10 6 cells in RNAlater ® C-14025


1.4 Endothelial Cells (Microvascular) 

Applications 


Oncology 


Cell-cell junction investigation 


Features 

Von Willebrand factor (vWF) positive 

CD31 positive 

Smooth muscle α-actin negative 


The walls of capillaries are composed of a 

single layer of microvascular endothelial 

cells. This layer is so thin that molecules 

such as oxygen, water, and lipids can pass 

through it by diffusion and enter the surrounding 

tissues. Waste products such 

as carbon dioxide and urea, on the other 

hand, diffuse back into the blood to be 

carried away. Because of their location 

at the interface between the circulating 

fluid in the lumen and the surrounding 

tissue, microvascular endothelial cells are 

highly active and closely involved in numerous 

physiological processes. 

Microvascular endothelial cells differ in 

morphologies and properties according 

to the tissues supplied by the capillaries. 

Therefore, PromoCell microvascular endothelial 

cells are available from dermal, 

lung, cardiac, uterine, bladder, and brain 

tissues. 

Available endothelial cell types (microvascular): 

HDMEC (see page 15) 

HDBEC (see page 16) 

HDLEC (see page 17) 

HBdMEC (see page 18) 

HCMEC (see page 18) 

HPMEC (see page 19) 

HUtMEC (see page 19) 

HBMEC (see page 20) 

Fig. 1: HDMEC culture stained for vWF 

(Factor VIII antigen). Nuclei counterstained 

with DAPI. 

Fig. 2: HDMEC culture stained for CD31 

antigen. Nuclei counterstained with DAPI. 



Human Dermal Microvascular 

Endothelial Cells (HDMEC) 

HDMEC are isolated from the dermis of 

juvenile foreskin and adult skin (different 

locations). Since the dermis contains 

blood and lymphatic capillaries, HDMEC 

comprise Blood and Lymphatic Microvascular 

Endothelial Cells. Both have a 

common origin and can be identified by 

several markers. 

Beside standard HDMEC, pre-screened 

HDMEC are also available. They have 

been positively tested for VEGF response 

using PromoCell´s unique 3D-Angiogenesis 

Assay (see page 59). VEGF is an 

important signaling protein involved in 

both vasculogenesis and angiogenesis. 

In vitro, VEGF has been shown to stimulate 

endothelial cell mitogenesis, cell 

migration, sprouting, and microvascular 

permeability. 



(see page 70) 


(see page 70) 




HDMEC Pellet (see page 97) 

HDMEC adult Pellet (see page 97) 

HDMEC pre-screened Pellet 

(see page 97) 

Human Pericytes (see page 49) 

Angiogenesis Assays and Kits 

(see page 59-63) 




CD31 Antibody 


Fig. 3: HDMEC culture in phase contrast. 



Human Dermal Microvascular Endothelial 

Cells (HDMEC) juvenile foreskin 



C-12210 

C-12260 

HDMEC Pellet 1x10 6 cells in RNAlater ® C-14015 


Cells (HDMEC) adult donor 



C-12212 

C-12262 

HDMEC adult Pellet 1x10 6 cells in RNAlater ® C-14016 


Cells (HDMEC) pre-screened 



C-12215 

C-12265 

HDMEC pre-screened Pellet 1x10 6 cells in RNAlater ® C-14017

16 




Human Dermal Blood Endothelial 

Cells (HDBEC) 

HDBEC are isolated from the dermis of 


locations) from a single donor*. 

The cells are routinely analyzed by flow 

cytometric analysis and immunofluorescent 

staining: they are > 95% CD31 

positive (see Fig. 4) and stain positive for 

von Willebrand factor. In addition, they 

are > 90% negative for podoplanin 

(see Fig. 5) and stain negative for 

smooth muscle specific α-actin. 

Blood Endothelial Cells have a key function 

in physiological processes like vessel 

tonus, capillary permeability, blood coagulation, 

fibrolysis, and angiogenesis. 

They are also useful for disease studies 

of atherosclerosis, tumorigenesis, and 

thrombosis. 



(see page 70) 




HDBEC Pellet (see page 97) 

HDBEC adult Pellet (see page 97) 

Human Dermal Lymphatic Endothelial 




Podoplanin, CD31, and Prox-1 

Antibodies (see www.promokine.info) 

Count 

75 

CD31 negative 

CD31 positive 

Fig. 4: Flow cytometric analysis of PromoCell HDBEC. 

98% of the cells are CD31 positive . 

50 

25 

0 

0 

10 10 2 10 3 10 4 

CD31 


Count 

75 

Podoplanin negative 

Podoplanin positive 

Fig. 5: Flow cytometric analysis of PromoCell HDBEC. 

91% of the cells are podoplanin negative. 

50 

25 

0 

0 

10 10 2 10 3 10 4 


Podoplanin 



Human Dermal Blood Endothelial Cells 

(HDBEC) juvenile foreskin 



C-12211 

C-12214 

HDBEC Pellet 1x10 6 cells in RNAlater ® C-14018 

Human Dermal Blood Endothelial Cells 

(HDBEC) adult donor 



C-12225 

C-12226 

HDBEC adult Pellet 1x10 6 cells in RNAlater ® C-14019 

*Human Dermal Lymphatic Microvascular Endothelial Cells (HDLEC) from the same donor are available on request.


Human Dermal Lymphatic 

Endothelial Cells (HDLEC) 

HDLEC are isolated from the dermis of 


locations) from a single donor*. The cells 

are routinely analyzed by flow cytometric 

analysis and immunofluorescent staining: 

> 95% of the cells are CD31 positive and 

podoplanin positive (see Fig. 6 and 7). 

In addition, the cells stain positive for 

Prox-1. 

Lymphatic vessels transport excess fluids 

from tissues to the circulatory system and 

are a major component of the immune 

system. The transported fluid is nearly 

cell free. The vessels are highly permeable, 

because they lack a continuous 

basal membrane. Lymphatic endothelial 

cells are involved in pathological alterations 

of the lymphatic system. During 

tumor lymphangiogenesis, the lymphatic 

endothelial cells build new vessels that 

infiltrate tumors, attract tumor cells, and 

induce tumor cell metastasis. 



(see page 70) 




HDLEC Pellet (see page 97) 

HDLEC adult Pellet (see page 97) 






Podoplanin, CD31, and Prox-1 

Antibodies (see www.promokine.info) 



Count 

75 

CD31 negative 

CD31 positive 

Fig. 6: Flow cytometric analysis of PromoCell HDLEC. 

99% of the cells are CD31 positive. 

50 

25 

0 

0 10 10 2 10 3 10 4 

CD31 


Fig. 7: Flow cytometric analysis of PromoCell HDLEC. 

96% of the cells are podoplanin positive. 

Count 

75 

Podoplanin negative Podoplanin positive 

50 

25 

0 

0 10 10 2 10 3 10 4 

Podoplanin 

Cell Lab Quanta SC T M , Beckman Coulter, Inc. 




Cells (HDLEC) juvenile foreskin 



C-12216 

C-12218 

HDLEC Pellet 1x10 6 cells in RNAlater ® C-14020 


Cells (HDLEC) adult donor 



C-12217 

C-12219 

HDLEC adult Pellet 1x10 6 cells in RNAlater ® C-14021 

*Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.

18 


Human Bladder Microvascular 

Endothelial Cells (HBdMEC) 




HBdMEC Pellet (see page 97) 

(see page 70) 




HBdMEC are isolated from the human 

bladder microvasculature. The cells are 

routinely analyzed by immunofluorescent 

staining: they stain positive for 

CD31 and von Willebrand factor and 


(see page 70) 




CD31 Antibody 


negative for smooth muscle α-actin. 

HBdMEC are useful in studying the 

role of microvascular endothelial 

cells in the control of inflammation, 

capillary exchange, tissue growth, and 

angiogenesis. 



Human Bladder Microvascular Endothelial 

Cells (HBdMEC) 



C-12291 

C-12292 

HBdMEC Pellet 1x10 6 cells in RNAlater ® C-14026 


Endothelial Cells (HCMEC) 

HCMEC are isolated from heart ventricles 

from single donors. The cells are 

routinely analyzed by immunofluorescent 




HCMEC intensively interact with cardiomyocytes 

and therefore have a characteristic 

phenotype different from other 

microvascular endothelial cells. HCMEC 

play an important role in the physiological 

regulation of coronary blood flow 

and capillary exchange. Medical conditions 

such as hypertrophy, ischemia, 

hypertension, and diabetes mellitus are 

associated with endothelial dysfunction. 



(see page 70) 


(see page 70) 




HCMEC Pellet (see page 97) 

Human Cardiac Myocytes (see page 9) 


(see page 26) 



CD31 Antibody 








C-12285 

C-12286 

HCMEC Pellet 1x10 6 cells in RNAlater ® C-14029


Human Pulmonary Microvascular 

Endothelial Cells (HPMEC) 




HPMEC Pellet (see page 98) 

(see page 70) 

Human Pulmonary Fibroblasts 

HPMEC are isolated from the lung from 

a single donor. The cells are routinely 

analyzed by immunofluorescent staining: 

they stain positive for CD31 and 

von Willebrand factor and negative for 


(see page 70) 



(see page 26) 

Human Small Airway Epithelial Cells 

(see page 22) 





smooth muscle α-actin. 

CD31 Antibody 

The lung has a vast endothelial surface 


area, which is essential for the exchange 

of gases. HPMEC are most appropriate 

for studying human lung diseases. 







C-12281 

C-12282 

HPMEC Pellet 1x10 6 cells in RNAlater ® C-14027 

Human Uterine Microvascular 

Endothelial Cells (HUtMEC) 

HUtMEC are isolated from the myometrium 

(middle layer of the uterine wall) 

from a single donor, who has not been 

pre-treated with hormones. The cells 

are routinely analyzed by immunofluorescent 




During pregnancy HUtMEC propagate 

intensely and change their phenotype 

and expression pattern in response to 

sex steroids. This includes up-regulation 

of HUtMEC nitric oxide synthase activity, 

modulation of adhesion molecule 

expression, and an increase in endothelial 

cell survival and angiogenic activity. 

HUtMEC are involved in medical conditions 

such as uterine fibroma or adenomyosis. 



(see page 70) 


(see page 70) 




HUtMEC Pellet (see page 98) 

Human Uterine Fibroblasts 

(see page 27) 

Human Uterine Smooth Muscle Cells 

(see page 39) 



CD31 Antibody 








C-12295 

C-12296 

HUtMEC Pellet 1x10 6 cells in RNAlater ® C-14028

20 


Human Brain Microvascular 

Endothelial Cells (HBMEC) 

Brain microvascular endothelial cells are 

highly specialized cells responsible for 

Therefore, PromoCell HBMEC are well 

suited for studying brain endothelial cell 

the formation and functional properties 

functions like active transport systems 



Human Brain Microvascular Cells 

(HBMEC) are isolated from cortical 

tissue (see Fig. 8) from a single donor. 

They are routinely analyzed by flow 

cytometry and immunofluorescent stai- 

of the Blood-Brain Barrier (BBB). The 

BBB is characterized by cellular tight 

junctions and active transport systems 

which allow the selective transport of 

substances into the brain, and prevent 

and drug uptake. Furthermore, neuroinflammation 

studies as well as tight 

junction research can be performed 

with HBMEC. 

ning. The cells are positive for CD31 and 

the entrance of unwanted substances. 

von Willebrand factor and negative for 

smooth muscle α-actin and CD90. 

Cerebral cortex 

Thalamus 

Corpus callosum 

Hypothalamus 

Pituitary gland 

Cerebellum 

Spinal cord 

Fig. 8: Schematic structure of the brain 

Fig. 9: HBMEC in phase contrast. 



Human Brain Microvascular Endothelial 

Cells (HBMEC) 



coming soon 

coming soon 

C-12287 

C-12288 

HBMEC Pellet 1x10 6 cells in RNAlater ® coming soon C-14032


1.5 Epithelial Cells 

Applications 


Toxicological research 

Wound healing research 

Oncology 

Cell differentiation studies 

Respiratory disease research 

Features 

Cytokeratin positive 

Available from different tissues 


Typically, epithelial tissue is made of 

tightly packed cells with little intercellular 

space and is arranged in flat sheets 

forming effective barriers between the 

underlying tissues and the external environment. 

PromoCell offers epithelial cells from different 

locations: 

Airways: Nasal mucosa, trachea, bronchi, 

and distal respiratory tract 

Breast: Mammary gland 

Kidney: Whole kidney and renal cortex 

Placenta: Amniotic membrane 

Fig. 1: HTEpC culture in phase contrast. 

Note the cuboidal shape typical for airway 

epithelial cells. 



The epithelium is the interface between the 

body and the external environment. It covers 

all outside surfaces and inside lumina 

e.g. the inner lining of the respiratory 

tract. In addition, epithelial cells also make 

up exocrine and endocrine glands. The 

functions of epithelial cells are diverse and 

include absorption, secretion, protection, 

transcellular transport, and sensation. 

Available Epithelial Cells: 

HNEpC (see page 21) 

HTEpC (see page 21) 

HBEpC (see page 22) 

HSAEpC (see page 22) 

HMEpC (see page 23) 

HREpC (see page 23) 

HRCEpC (see page 24) 

HPlEpC (see page 24) 

Fig. 2: HTEpC culture stained for cytokeratin. 

Nuclei counterstained with 

DAPI. 

Human Nasal Epithelial Cells 

(HNEpC) 

HNEpC are isolated from normal human 

nasal mucosa and stain positive for cytokeratin. 

The epithelium in the nasal cavity cleans, 

humidifies, and warms inhaled air. In addition, 

it produces mucus, which binds 

particles that are subsequently transported 

to the pharynx by cilia on the 

epithelial cells. HNEpC are useful for in 

vitro studies of these processes. 


Airway Epithelial Cell Growth Medium 

(see page 71) 




HNEpC Pellet (see page 98) 





Human Nasal Epithelial Cells (HNEpC) 



C-12620 

C-12621 

HNEpC Pellet 1x10 6 cells in RNAlater ® C-14062 

Human Tracheal Epithelial Cells 

(HTEpC) 

HTEpC are isolated from the surface 

epithelium of human trachea and stain 

positive for cytokeratin. 

The respiratory epithelia are responsible 

for the lubrication of the lungs, 

the maintenance of humidity, and the 

cleaning of the respiratory tract. They 

are an important target for drugs, toxins, 

and carcinogens. In addition, they 

are involved in many diseases such as 

cystic fibrosis. Consequently, HTEpC are 

useful for investigating the function and 

pathology of the respiratory system. 



(see page 71) 




HTEpC Pellet (see page 98) 

Human Tracheal Smooth Muscle Cells 

(see page 37) 


(see PromoKine catalog)

22 






Human Tracheal Epithelial Cells (HTEpC) 



C-12644 

C-12645 

HTEpC Pellet 1x10 6 cells in RNAlater ® C-14064 

Human Bronchial Epithelial Cells 

(HBEpC) 

HBEpC are isolated from the surface 

epithelium of human bronchi and stain 

positive for cytokeratin. 

The respiratory epithelia are responsible 

for the lubrication of the lungs, 

the maintenance of humidity, and the 

cleaning of the respiratory tract. They 

are an important target for drugs, toxins, 

and carcinogens. In addition, they 

are involved in many diseases such as 

cystic fibrosis. Consequently, HBEpC are 

useful for investigating the function and 

pathology of the respiratory system. 



(see page 71) 




HBEpC Pellet (see page 97) 

Human Bronchial Smooth Muscle 






Human Bronchial Epithelial Cells (HBEpC) 



C-12640 

C-12641 

HBEpC Pellet 1x10 6 cells in RNAlater ® C-14063 

Human Small Airway Epithelial 

Cells (HSAEpC) 

HSAEpC are isolated from the distal portion 

of the human respiratory tract in the 

1 mm bronchiole area. They stain positive 

for cytokeratin. 

The distal respiratory tract mainly consists 

of pulmonary alveoli, which are 

spherical outcroppings of the respiratory 

bronchioles and are the primary sites of 

gas exchange with the blood. 

HSAEpC are qualified for functional 

studies to investigate disorders such as 

asthma or pulmonary inflammation. 

Fig. 3: HSAEpC culture in phase contrast. 


Small Airway Epithelial Cell 

Growth Medium (see page 72) 




HSAEpC Pellet (see page 98) 




(see page 26) 






(HSAEpC) 



C-12642 

C-12643 

HSAEpC Pellet 1x10 6 cells in RNAlater ® C-14065


Human Mammary Epithelial Cells 

(HMEpC) 

milk secretion (lactation) begins. 

Since mammary gland cells can easily be 


HMEpC Pellet (see page 97) 

induced to grow and multiply by hor- 


HMEpC are isolated from the human 

adult mammary gland and stain positive 

for cytokeratin. 

Hormones extensively control the development 

of the mammary glands. 

mones, HMEpC are very well suited for 

developmental investigations. In addition, 

they are useful for breast cancer in 

vitro research since breast cancer usually 

originates in the lobules or ducts of the 




Throughout puberty, the glands begin to 

mammary glands. 

develop in females in response to ovarian 

hormones. Further on during pregnancy, 


rising levels of estrogen and progester- 

Mammary Epithelial Cell Growth 

one cause additional growth and differ- 

Medium (see page 73) 

entiation. Finally, due to the presence of 


the hormone prolactin in late pregnancy, 


Fig. 4: HMEpC culture in phase contrast. 




(HMEpC) 



C-12650 

C-12651 

HMEpC Pellet 1x10 6 cells in RNAlater ® C-14066 

Human Renal Epithelial Cells 

(HREpC) 

HREpC are isolated from the adult kidney 

and stain positive for cytokeratin. 

Kidneys have a very complex structure 

and contain diverse epithelia such as 

those in the convoluted tubules, the 

cortical collecting ducts, the calyxes, 

Renal cortex 

and the renal pelvis. The epithelial cells 

present in the distinct ducts and tubules 

differ in their morphology and physiological 

function. Consequently, HREpC 

are comprised of a heterogeneous population 

of epithelial cells. 

Important physiological processes like 

osmoregulation and excretion can be 

studied with HREpC in vitro. 


Renal Epithelial Cell Growth 

Medium 2 (see page 74) 




HREpC Pellet (see page 98) 

Human Renal Cortical Epithelial Cells 

(see page 24) 



Renale capsule 

Renal medulla 

Renal calices 

Renal pelvis 

Ureter 

Fig. 5: Schematic structure of a kidney. 

Fig. 6: HREpC culture in phase contrast.

24 






Human Renal Epithelial Cells (HREpC) 



C-12665 

C-12666 

HREpC Pellet 1x10 6 cells in RNAlater ® C-14067 

Human Renal Cortical Epithelial 

Cells (HRCEpC) 

HRCEpC are isolated from the cortex of 

the human kidney and stain positive for 

cytokeratin. The renal cortex is the outer 

portion of the kidney between the renal 

capsule and the renal medulla. It contains 

the renal corpuscles, the proximal 

and distal convoluted tubules, and the 

cortical collecting ducts. 

Important physiological processes like 

osmoregulation and excretion can be 

studied with HRCEpC in vitro. 







HRCEpC Pellet (see page 98) 


(see page 23) 






(HRCEpC) 



C-12660 

C-12662 

HRCEpC Pellet 1x10 6 cells in RNAlater ® C-14068 

Human Placental Epithelial Cells 

(HPlEpC) 

HPlEpC are isolated from the amniotic 

membrane and stain positive for cytokeratin. 

Placental Epithelial Cells are of fetal origin 

and form a single layer at the inner 

surface of the amnion. They protect the 

fetus against maternal leukocytes, neoplastic 

cells, and pathogens. Cultured 

HPlEpC are suitable for physiological and 

pathological studies of amniotic functions. 


Placental Epithelial Cell Growth 





Human Villous Mesenchymal 

Fibroblasts (see page 27) 

HPlEpC Pellet (see page 98) 





Human Placental Epithelial Cells (HPlEpC) 



C-12395 

C-12397 

HPIEpC Pellet 1x10 6 cells in RNAlater ® C-14069


1.6 Fibroblasts 

Applications 


Tissue engineering research 

Regeneration studies 

Toxicological research 

Oncology 

Epithelial-mesenchymal interaction 

studies 

Features 

CD90 positive 


Available from different tissues 

Fibroblasts are the most common cells 

in connective tissue. Their main function 

is to maintain the structural integrity of 

the connective tissue by continuously 

secreting extracellular matrix proteins 

like collagens, glycosaminoglycans, 

and glycoproteins. The composition of 

the extracellular matrix determines the 

physical properties of connective tissues. 

Fibroblasts are morphologically heterogeneous 

with diverse appearances 

depending on their location in the body 

and their activity. Injury of tissue displays 

a proliferative stimulus for fibroblasts and 

induces them to produce wound healing 

proteins. Therefore, fibroblasts are well 

suited for wound healing studies. 

Available Fibroblasts: 

NHDF (see page 25) 

HPF (see page 26) 

HAoAF (see page 26) 

HCF (see page 26) 

HUF (see page 27) 

HVMF (see page 27) 

Fig. 1: NHDF culture in phase contrast. 

Fig. 2: NHDF culture stained for CD90. 

Nuclei counterstained with DAPI. 



Normal Human Dermal Fibroblasts 

(NHDF) 

NHDF are isolated from the dermis of 

juvenile foreskin or adult skin from different 

locations like the face, the breasts, 

the abdomen, and the thighs*. They can 

be used for wound healing studies and 

dermatological research to investigate 

diseases like scleroderma, fibrosarcoma, 

fibrosis, xeroderma pigmentosum, and 

histiocytoma. Moreover, fibroblasts are 

important for cancer research, tissue 

regeneration, and tissue engineering 

studies. 

Recommended media & reagents for 

NHDF juvenile foreskin 

Fibroblast Growth Medium 

(see page 76) 



Recommended media & reagents for 

NHDF adult donor 

Fibroblast Growth Medium 2 

(see page 76) 




NHDF Pellet (see page 98) 

NHDF adult Pellet (see page 98) 

Normal Human Epidermal 

Keratinocytes (see page 30) 


Melanocytes (see page 31) 



FGF-1 and FGF-2 (see page 106) 



Normal Human Dermal Fibroblasts (NHDF) 

juvenile foreskin 



C-12300 

C-12350 

NHDF Pellet 1x10 6 cells in RNAlater ® C-14030 

Normal Human Dermal Fibroblasts (NHDF) 

adult donor 



C-12302 

C-12352 

NHDF adult Pellet 1x10 6 cells in RNAlater ® C-14031 

* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are 

available on request.

26 



(HPF) 

pulmonary hypertension can also be investigated 

with cultured HPF. 


HPF Pellet (see page 98) 


HPF are isolated from human lung tis- 





sue. They are often used for studying 

lung tissue repair, tissue remodeling after 

injury, or the response after pulmonary 

inflammation. Fibroblast disorders like 

pulmonary fibrosis or hypoxia-induced 


(see page 76) 




(see page 22) 






Human Pulmonary Fibroblasts (HPF) 



C-12360 

C-12361 

HPF Pellet 1x10 6 cells in RNAlater ® C-14035 

Human Aortic Adventitial 

Fibroblasts (HAoAF) 

HAoAF are isolated from human aortic 

adventitial tissue. HAoAF can be used 

for studies concerning vascular diseases 

e.g. neointimal formation, vascular remodeling, 

atherosclerosis, and hypertension. 

They are also often used for the 

examination of fibroblast disorders like 

fibrosis or other diseases linked to either 

imperfect or excessive accumulation of 

fibroblasts. 



(see page 76) 




HAoAF Pellet (see page 97) 


(see page 13) 


(see page 35) 







(HAoAF) 



C-12380 

C-12381 

HAoAF Pellet 1x10 6 cells in RNAlater ® C-14037 

Human Cardiac Fibroblasts (HCF) 

HCF are isolated from the ventricles of 

an adult heart. They play a central role in 

the maintenance of the extracellular matrix 

in the normal heart and the synthesis 

of growth factors and cytokines. Under 

pathophysiological conditions, cardiac 

fibroblasts are involved in scar formation 

after myocardial infarction, cardiac fibrosis, 

and cardiac hypertrophy. HCF can be 

used to study such processes in vitro. 



(see page 77) 




HCF Pellet (see page 97) 

Human Cardiac Myocytes (see page 9) 








Human Cardiac Fibroblasts (HCF) 



C-12375 

C-12377 

HCF Pellet 1x10 6 cells in RNAlater ® C-14036


Human Uterine Fibroblasts (HUF) 




HUF Pellet (see page 98) 

HUF are isolated from the uterine wall. 

(see page 76) 


During pregnancy, the uterine wall un- 


Endothelial Cell (see page 19) 

dergoes deep but reversible structural 

and biochemical changes. The uterine 

wall is the target of hormones and 

growth factors that act simultaneously 

and lead to extensive modifications of 



(see page 39) 






the extracellular matrix. This and other 

morphological and biochemical effects 

can be studied in vitro using HUF. 



Human Uterine Fibroblasts (HUF) 



C-12385 

C-12386 

HUF Pellet 1x10 6 cells in RNAlater ® C-14038 


Fibroblasts (HVMF) 

HVMF are isolated from human villous 

placental tissue. They can be used as 

model systems in organ development 

research. In addition, they are useful for 

investigating the mechanism regulating 

human placental growth and function. 



(see page 76) 




HVMF Pellet (see page 98) 


(see page 24) 






Human Villous Mesenchymal Fibroblasts 

(HVMF) 



C-12390 

C-12391 

HVMF Pellet 1x10 6 cells in RNAlater ® C-14034

28 


1.7 Follicle Dermal Papilla Cells 

Applications 


Follicle Dermal Papilla Cell Growth 

Hair growth research 




Drug testing 

Toxicological testing 

Features 

Alkaline phosphatase positive 




HFDPC Pellet (see page 97) 


Fig. 1: HFDPC culture in phase contrast. 



Available from adult lateral scalp 

Available from donors with different 

hair colors 

Human Follicle Dermal Papilla Cells 

(HFDPC) are isolated from human der- 

Sebaceous gland 

mis originating from the lateral scalp. 

Information on donor hair and skin color 

is available for each lot. HFDPC stain 

Hair shaft 

Bulge 

positive for alkaline phosphatase. 

Dermal papillae are embedded in a 

laminin and collagen IV rich extracellular 

Matrix cells 

matrix at the base of the hair follicles. 

They are essential for the induction and 

maintenance of hair growth. Since they 

have androgen receptors, they can be 

Dermal papilla 

used for in vitro screening of androgen 

blocking reagents. 

Fig. 2: Diagram showing the location of the dermal papilla in the hair follicle. 




(HFDPC) 



C-12071 

C-12072 

HFDPC Pellet 1x10 6 cells in RNAlater ® C-14005


1.8 Hepatocytes 

Applications 

metabolism, and albumin synthesis. The 


cell adherence efficiency is at least 50%. 

Hepatocyte Growth Medium 

Drug testing 

(see page 79) 

Toxicological testing 

Metabolism studies 

Liver physiology research 

Liver pathology research 

Oncology 

The liver is the main metabolic organ 

in the body performing glycolysis, 

glycogenesis, amino acid metabolism, 

lipid metabolism, and ureagenesis. In 

addition, it also regulates the blood 

Hepatocyte Maintenance Medium 

(see page 79) 





Features 

sugar level. Furthermore, hepatocytes 

eliminate toxic substances soon after 


HHpC Pellet (see page 97) 

absorption since the nutrition rich blood 

PromoFectin-Hepatocyte Transfection 

Cryopreserved immediately after 

from the gastro-intestinal system first 


isolation 

has to pass the liver. This detoxifica- 

Collagen Type I from Rat Tail 

3 - 6 x 10 6 viable cells per cryovial 

tion consists of phase I and phase II 

(see page 105) 

Adherence > 50% 

reactions. The most important phase I 

Phase I reaction positive 

enzyme system is the cytochrome P450 

Phase II reaction positive 

system. Consequently, cultured human 

Albumin positive 

hepatocytes are perfectly suited for in 

vitro metabolism and toxicity/detoxi- 

Human Hepatocytes (HHpC) are iso- 

fication studies prior to preclinical or 

lated from the normal livers of single 

clinical tests. 

donors. After isolation, the cells are 

immediately cryopreserved and then 

Note: Under standard in vitro cell cul- 

intensively quality tested. Test cri- 

ture conditions, mature human hepato- 

teria are cell viability, cell adherence 

efficiency, phase I metabolism, phase II 

cytes usually do not survive for longer 

than 10-14 days and do not proliferate. 

Fig 1. HHpC culture in phase contrast. 

For more information please see 

www.promocell.com/knowledge-base 

Count 

37,5 

Living cells 

Dead cells 

Fig. 2: Flow cytometric analysis of PromoCell HHpC 

using 7-AAD staining. 77 % viable hepatocytes after 

thawing of the cryopreserved cells. 

25 

12,5 

0 

0 

10 10 2 10 3 10 4 


7-AAD 



Human Hepatocytes (HHpC) 3 - 6 x 10 6 cryopreserved cells C-12850 

HHpC Pellet 1x10 6 cells in RNAlater ® C-14085

30 


1.9 Keratinocytes 

Applications 

body and minimizes moisture loss. 


Keratinocytes are also able to produce 

NHEK.f Pellet (see page 98) 


a variety of cytokines, growth factors, 

NHEK.f pooled Pellet (see page 98) 



Epidermal development and 

differentiation studies 

Dermatological research 


Pharmaceutical testing 

interleukins and complement factors. 

Therefore keratinocytes are important 

for wound healing, inflammation, and 

immune response. 

NHEK adult Pellet (see page 98) 

NHEK adult, pooled Pellet 

(see page 98) 


(see page 25) 

Cosmetic and toxicological testing 



Oncology 

Keratinocyte Growth Medium 2 

Melanocytes (see page 31) 

Features 

(see page 80) 





Cytokeratin positive 

Available from juvenile foreskin or 

adult skin from different locations 

Available from single and pooled 

Stratum corneum 

Stratum granulosum 

donors 

Stratum spinosum 

Normal Human Epidermal Keratinocytes 

(NHEK) are available from single or 

pooled donors isolated from the epidermis 

Stratum basale 

of juvenile foreskin or adult skin from different 

locations like the face, the breasts, 

the abdomen, and the thighs*. They are 

Fig. 1: Structure of the epidermis. 

the major cell type in the epidermis, making 

up about 90% of the cells. 

Epidermal keratinocytes originate in the 

stratum basale and move up through 

the layers of the epidermis. During this 

movement, they undergo gradual differentiation 

and morphology changes until 

they reach the stratum corneum, where 

they form a layer of nucleus-free, flat, 

and highly keratinized squamous cells. 

Fig. 2: NHEK culture in phase contrast. 

Fig. 3: NHEK culture stained for Cyto- 

This layer forms an effective barrier to 

Note the cobblestone shape typical for 

keratin. Nuclei counterstained with 

the entry of infectious agents into the 

keratinocytes. 

DAPI. 




(NHEK) juvenile foreskin, single donor 



C-12001 

C-12002 

NHEK.f Pellet 1x10 6 cells in RNAlater ® C-14001 


(NHEK) juvenile foreskin, pooled 



C-12005 

C-12007 

NHEK.f pooled Pellet 1x10 6 cells in RNAlater ® new 

C-14003 


(NHEK) adult, single donor 



C-12003 

C-12004 

NHEK adult Pellet 1x10 6 cells in RNAlater ® C-14002 


(NHEK) adult, pooled 



C-12006 

C-12008 

NHEK adult, pooled Pellet 1x10 6 cells in RNAlater ® new 

C-14004 

* Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available 

on request. 

new 

new 

new 

new


1.10 Melanocytes 

Applications 

using either the serum-free, PMA (Phor- 

Melanocyte Growth Medium M2 

bol Myristate Acetate)-containing Mela- 

(see page 81) 

Dermatological research 

nocyte Growth Medium or the serum- 


Melanoma research 

Melanogenesis studies 

Cosmetic and toxicological testing 

Skin compound screening 

Features 

free and PMA-free Melanocyte Growth 

Medium M2. 

Since PMA is a tumor promoting mitogen, 

it can interfere with experimental 

approaches. Therefore, we recommend 

using cells isolated in Melanocyte 



NHEM.f Pellet (see page 98) 

NHEM.f M2 Pellet (see page 98) 

NHEM M2 adult Pellet (see page 98) 



Growth Medium M2 and also using this 


Available from juvenile foreskin or 

medium for cultivation. 

(see page 25) 

adult skin from different locations 


Available from light, moderate, and 


Keratinocytes (see page 30) 

dark skin types 

Melanocyte Growth Medium 


Donor hair and eye color available on 

(see page 81) 


request 

Mel-5 positive 

Cultured in PMA-containing or 

PMA-free Medium 

Stratum corneum 

Stratum granulosum 

Normal Human Epidermal Melanocytes 

(NHEM) are isolated from the epidermis 

Stratum spinosum 

of juvenile foreskin or adult skin from 

different locations including the face, the 

breasts, the abdomen, and the thighs*. 

They are located in the stratum basale, 

Stratum basale 

but branch out between the keratinocytes 

in suprabasal layers (see Fig. 1). 

About 5-10 % of the cells in the epidermis 

are melanocytes. 

Melanosoma Keratinocytes 

Melanocyte 

Fig. 1: Structure of the epidermis with 

the location of a melanocyte. 

The main purpose of melanocytes is 

the production of melanin, the protein 

responsible for the pigmentation of the 

skin, eyes, and hair. Melanin protects the 

cells in the skin and in deeper layers from 

the hazardous effects of UV radiation. It 

is produced and stored in melanosomes, 

which are located close to the melanocyte 

membrane, but it can also be transferred 

to neighboring keratinocytes. 

Fig. 2: NHEM cultured in Melanocyte 

Fig. 3: NHEM cultured in Melanocyte 

PromoCell melanocytes are isolated 

Growth Medium in phase contrast. 

Growth Medium M2 in phase contrast. 




(NHEM) juvenile foreskin 



C-12400 

C-12450 

NHEM.f Pellet 1x10 6 cells in RNAlater ® C-14040 


(NHEM) juvenile foreskin, cultured in M2 

Medium 



C-12402 

C-12452 

NHEM.f M2 Pellet 1x10 6 cells in RNAlater ® C-14042 


(NHEM) adult donor, cultured in M2 

Medium 



C-12403 

C-12453 

NHEM M2 adult Pellet 1x10 6 cells in RNAlater ® C-14043 

* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.

32 


1.11 Osteoblasts 

Applications 

Human Osteoblasts (HOB) are isolated 


from femoral trabecular bone tissue from 

Osteoblast Growth Medium 

Bone differentiation 

the knee or hip joint region (lot specific 

(see page 82) 



Bone remodeling 

Bone physiology research 

Oncology 

Inflammatory studies 

Osteoporosis research 

source information is available on request). 

They stain positive for alkaline 

phosphatase and can be mineralized using 

PromoCell Mineralization Medium. 

Osteoblasts are of mesenchymal origin 

Osteoblast Mineralization Medium 

(see page 82) 



Features 

and are mainly responsible for bone formation 

and remodeling. In vivo, they 


HOB Pellet (see page 98) 

produce a collagen type I rich extracellular 


From knee and hip femoral tissue 

matrix called the osteoid. 



During their development, osteoblasts 

Alkaline phosphatase positive 

undergo a maturation process called 

Tested for mineralization 

osteogenesis. When they become en- 

Growth and Mineralization Media 

cased in the bone matrix, they stop prolif- 

available 

erating and become osteocytes. 



Fig. 1: HOB culture in phase contrast. 

Fig. 2: Alkaline phosphatase activity in 

HOB stained with BCIP/NBT. 

Fig. 3: Mineralized HOB. Calcium deposits 

stained with Alizarin Red S. 



Human Osteoblasts (HOB) 



C-12720 

C-12760 

HOB Pellet 1x10 6 cells in RNAlater ® C-14071


1.12 Preadipocytes 

Applications 

Adipose tissue is crucial in energy storage 


and metabolic homeostasis. An increase 

Preadipocyte Growth Medium 

Proliferation/differentiation studies 

in adipose tissue results either from an 

(see page 83) 

Physiological and pathological 

research 

Fat metabolism research 

Obesity/diabetes studies 

Oncology (e.g. breast cancer) 

enlargement of mature adipocytes or 

from the differentiation of adipocyte 

precursor cells (preadipocytes) into new 

mature adipocytes. These preadipocytes 

are already present in adipose tissues. 

Preadipocyte Differentiation Medium 

(see page 83) 

Adipocyte Nutrition Medium 

(see page 83) 




Features 

They can proliferate throughout adult 

life and replace the cells that have dif- 


ferentiated into mature adipocytes. 


From subcutaneous and visceral 

Since this differentiation process is con- 

HWP subcutaneous Pellet 

adipose tissue 

trolled by a variety of growth factors and 

(see page 98) 

Available from different locations 

hormones, preadipocytes are suitable 

HWP visceral Pellet (see page 98) 


for the investigation of the physiologi- 


Differentiation capacity to adipocytes 

cal mechanisms controlling proliferation, 


Growth and Differentiation Media 

differentiation, and function of adipose 

available 

tissue. 

Human White Preadipocytes (HWP) are 

The PromoCell adipocyte cell culture 

isolated from adult subcutaneous or vis- 

system provides preadipocytes with op- 

ceral adipose tissue from different loca- 

timized growth media and serum-free 

tions (lot specific source information is 

differentiation media. 

available on request). 

Fig. 1: Left: Undifferentiated HWP (phase contrast). Middle: HWP after in vitro differentiation into adipocytes using the PromoCell 

Preadipocyte Differentiation Medium (phase contrast). Right: HWP after in vitro differentiation into adipocytes (Lipid droplets 

stained with Oil Red O). 



Human White Preadipocytes (HWP) 

subcutaneous 



C-12730 

C-12731 

HWP subcutaneous Pellet 1x10 6 cells in RNAlater ® C-14072 


visceral 



C-12732 

C-12733 

HWP visceral Pellet 1x10 6 cells in RNAlater ® C-14073

34 


1.13 Skeletal Muscle Cells 

Applications 

liferate and fuse with damaged muscle 


fibers or with one another forming new 

Skeletal Muscle Cell Growth Medium 

Gene therapy research 

myotubes. 

(see page 84) 



Transplantation research 

Developmental/differentiation 

studies 

Tissue repair research 

Pharmacological testing 

SkMC are optimal for in vitro muscle 

studies. They proliferate very well in 

the mitogen-rich PromoCell Skeletal 

Muscle Cell Growth Medium. Fusion to 

Skeletal Muscle Cell Differentiation 




Neuromuscular disease research 

myotubes with typical multinucleated 


Features 

syncytia can be induced by using the 

PromoCell Skeletal Muscle Cell Differen- 

SkMC Pellet (see page 98) 


tiation Medium. 


Sarcomeric myosin positive 

Available from different muscles 

Differentiation capacity to multi- 

nucleate syncytia 

Growth and Differentiation Media 

available 

Human Skeletal Muscle Cells (SkMC) are 

isolated from different skeletal muscles 

(e.g. Musculus pectoralis major) from 

Fig. 1: SkMC culture in phase contrast. 

adult single donors (lot specific source 

information is available on request). 

They are positive for sarcomeric myosin 

and negative for smooth muscle specific 

α-actin. 

New skeletal muscle cells originate from 

quiescent satellite cells, which are located 

in the muscle fibers between the basal 

lamina and the sarcolemma. Quiescent 

satellite cells are activated by stimuli such 

Fig. 2: Differentiated SkMC culture in 

as muscle damage. After activation, the 

phase contrast. Arrows indicate multi- 

cells, now called myoblasts, start to pro- 

nucleated syncytia. 





Human Skeletal Muscle Cells (SkMC) 



C-12530 

C-12580 

SkMC Pellet 1x10 6 cells in RNAlater ® C-14060


1.14 Smooth Muscle Cells 

Applications 

the walls of blood vessels and hollow organs 

like the bladder, the uterus, and the 

Organ specific drug testing 

gastrointestinal tract and are responsible 

Cell-extracellular matrix interaction 

studies 

Physiological and biochemical studies 

Oncology 

Pathological studies 

(e.g. hypertension) 

for involuntary movements like peristaltic 

contractions, which propel food along 

the gastrointestinal tract. 

PromoCell Smooth Muscle Cells are 

available from the aorta, the coronary 

artery, the pulmonary artery, the umbili- 

Fig. 1: HTSMC culture in phase contrast. 




cal artery, the trachea, the bronchi, the 

bladder, the prostate, and the uterus. 

Features 

Available Smooth Muscle Cells: 

Smooth muscle α-actin positive 

HAoSMC (see page 35) 

CD90 negative 

HCASMC (see page 36) 

Routinely morphology tested 

HPASMC (see page 36) 


HUASMC (see page 37) 

HTSMC (see page 37) 

Smooth muscle cells, which form non- 

HBSMC (see page 38) 

striated muscles, are structurally and 

HBdSMC (see page 38) 

Fig. 2: HTSMC culture stained for smooth 

functionally different from skeletal and 

HPrSMC (see page 39) 

muscle α-actin. Nuclei counterstained 

cardiac muscle cells. They are located in 

HUtSMC (see page 39) 

with DAPI. 

Human Aortic Smooth Muscle 

Cells (HAoSMC) 

HAoSMC are isolated from plaque-free 

regions of the human aorta (see Fig. 3) 

and stain positive for smooth muscle 

α-actin. The pulsatile pressure produced 

by the heart causes a cyclic distention 

Tunica externa 

(Connective tissue) 

of the aorta. The smooth muscle cells in 

the aortic wall subsequently contract it 

again. Changes in the arterial wall, associated 

with vascular diseases such as ath- 

Tunica media 

(Smooth muscle cells) 

erosclerosis and hypertension, strongly 

influence this process. 

HAoSMC are suitable for studying the 

role of smooth muscle cells under normal 

or disease conditions in vitro. 

Tunica intima 

(Endothelial cells) 

Fig. 3: Diagram of a blood vessel. 


Smooth Muscle Cell Growth 




HAoSMC Pellet (see page 97) 

(see page 13) 





(see page 26) 





(HAoSMC) 



C-12533 

C-12532 

HAoSMC Pellet 1x10 6 cells in RNAlater ® C-14053

36 



Muscle Cells (HCASMC) 



HCASMC are isolated from human coronary 

arteries (see Fig. 4) and stain positive 

for smooth muscle α-actin. Coronary 

arteries supply the heart with blood 

and obstruction in atherosclerosis causes 

myocardial infarction. 

Left main coronary 

artery 

Circumflex coronary 

artery 

HCASMC are qualified to study the 

function of smooth muscle cells in the 

development of atherosclerotic plaques 

or in other diseases like hypertension. 

They are also useful for stent-graft compatibility 

testing. 






Right coronary 

artery 

Fig. 4: Location of the coronary arteries. 


HCASMC Pellet (see page 97) 

Human Coronary Artery Endothelial 

Left anterior descending 

coronary artery 






Human Coronary Artery Smooth Muscle 

Cells (HCASMC) 



C-12511 

C-12512 

HCASMC Pellet 1x10 6 cells in RNAlater ® C-14052 


Muscle Cells (HPASMC) 



HPASMC are isolated from human pulmonary 

arteries and stain positive for 

smooth muscle α-actin. They are suited 

for studying the function of pulmonary 

arteries under normal and disease conditions 

(e.g. vasoconstriction induced 

by alveolar hypoxia). They are also useful 

for physiological studies of vascular 

smooth muscle cells. 





HPASMC Pellet (see page 98) 

Human Pulmonary Artery Endothelial 




Fig. 5: Top: HPASMC culture in phase 

contrast. Bottom: HPASMC culture 

stained for smooth muscle α-actin. 

Nucleus counterstained with DAPI. 



Human Pulmonary Artery Smooth Muscle 

Cells (HPASMC) 



C-12521 

C-12522 

HPASMC Pellet 1x10 6 cells in RNAlater ® C-14051



Muscle Cells (HUASMC) 




HUASMC Pellet (see page 98) 



HUASMC are isolated from the arteries 

of the umbilical cord and stain positive 

for smooth muscle α-actin. Physiological 

and biochemical processes of vascular 

diseases can be analyzed with HUASMC 








in culture. 



Human Umbilical Artery Smooth Muscle 

Cells (HUASMC) 



C-12500 

C-12550 

HUASMC Pellet 1x10 6 cells in RNAlater ® C-14050 

Human Tracheal Smooth Muscle 

Cells (HTSMC) 

HTSMC are isolated from the trachea 

(see Fig. 6) from single donors and stain 

positive for smooth muscle α-actin. They 

connect the endings of the tracheal 

cartilage by a contractile bridge called 

the paries membranaceus and consequently 

regulate the airway lumen. Under 

pathological conditions like asthma, 

the amount of smooth muscle cells in 

the trachea walls is increased leading to 

smooth muscle hypertrophy or hyperplasia. 

Hence, HTSMC are suitable for in 

vitro studies of asthma and other pulmonary 

diseases. 

Paries 

membranaceus 

Hyaline cartilage 

Mucosa 







HTSMC Pellet (see page 98) 

Fig. 6: Cross section through the trachea with c-shaped cartilage connected by the 

tracheal muscles. 


(see page 21) 






(HTSMC) 



C-12565 

C-12566 

HTSMC Pellet 1x10 6 cells in RNAlater ® C-14056

38 



Cells (HBSMC) 

leading to smooth muscle hypertrophy 

or hyperplasia. Hence, HBSMC are suit- 


HBSMC Pellet (see page 97) 

able for in vitro studies of asthma and 




HBSMC are isolated from the bronchi 

from single donors and stain positive for 

smooth muscle α-actin. They connect 

the endings of the bronchial cartilage 

and consequently regulate the airway lu- 

other pulmonary diseases. 




(see page 22) 



men. Under pathological conditions like 


asthma, the amount of smooth muscle 


cells in the bronchi walls is increased 



Human Bronchial Smooth Muscle Cells 

(HBSMC) 



C-12561 

C-12562 

HBSMC Pellet 1x10 6 cells in RNAlater ® C-14055 

Human Bladder Smooth Muscle 

Cells (HBdSMC) 

HBdSMC are isolated from the detrusor 

muscle from the urinary bladder (see 

Fig. 7) of single donors and stain positive 

for smooth muscle α-actin. The thick 

bladder wall contains smooth muscle cells 

arranged in muscle bundles, which empty 

the bladder by coordinated contraction. 

HBdSMC can be used for in vitro studies 

of physiological processes as well as the 

effect of drugs on the bladder. 

Mucosa 

Submucosa 

Detrusor Muscle 

Adventitia 






Fig. 7: Diagram of the bladder. 


HBdSMC Pellet (see page 97) 







Human Bladder Smooth Muscle Cells 

(HBdSMC) 



C-12571 

C-12572 

HBdSMC Pellet 1x10 6 cells in RNAlater ® C-14057


Human Prostate Smooth Muscle 

Cells (HPrSMC) 

HPrSMC are isolated from the prostate 

from single donors and stain positive 

for smooth muscle a-actin. The prostate 

produces secretions that get into 

the urethra through contraction of the 


Prostate smooth muscle cells can be involved 

in prostitis and benign prostatic 

hyperplasia (BPH). HPrSMC can be used 

to study these prostate diseases or physiological 

processes in vitro. 

Lateral lobes 

Capsule 

Verumontanum 

Ejaculatory duct 








Fig. 8: Diagram of the prostate. 


HPrSMC Pellet (see page 98) 





Human Prostate Smooth Muscle Cells 

(HPrSMC) 



C-12573 

C-12574 

HPrSMC Pellet 1x10 6 cells in RNAlater ® C-14058 

Human Uterine Smooth Muscle 

Cells (HUtSMC) 

HUtSMC are isolated from the myometrium 

(the middle layer of the uterine wall, 

see Fig. 9) and stain positive for smooth 

muscle α-actin. During pregnancy, hormones 

and growth factors cause an extensive 

hypertrophy of uterine smooth 

muscle cells. 

This and other morphological and biochemical 

effects on the uterus can be 

studied in vitro using HUtSMC. 






Uterine cavity 

Vagina 

Fig. 9: Diagram of the uterus. 


HUtSMC Pellet (see page 98) 

Uterine tube 

Perimetrium (peritoneum) 

Myometrium (muscle) 

Endometrium (epithelium) 

Cavity of cervix 



Fig. 9: Diagram of the uterus. 




(HUtSMC) 



C-12575 

C-12576 

HUtSMC Pellet 1x10 6 cells in RNAlater ® C-14059

40 


Overview Human Primary Cells 



Product 

Human Cardiac Myocytes 

(HCM) 

Cat. No. 

Cryopr. Cells 

Recommended 

Culture Media* 

Plating Density 

C-12810 C-22070 10,000 - 15,000 

cells per cm² 

Human Chondrocytes (HCH) C-12710 C-27101 10,000 - 20,000 


Passage after 

Thawing 

Marker 

Population 

Doublings 

Page 

> 15 9 

P2 Sarcomeric α-actinin + 

myosin + 

Slow muscle 

P2 Differentiation tested > 10 10 

Human Umbilical Vein 

Endothelial Cells (HUVEC) 

single donor 

C-12200 C-22010 

C-22011 

5,000 - 10,000 


P1 vWF + 

CD31 + 

Dil-Ac-LDL uptake + 

> 15 11 



pooled 

C-12203 C-22010 

C-22011 

5,000 - 10,000 


P1 vWF + 

CD31 + 


> 15 11 



pre-screened 

C-12205 C-22010 

C-22011 

5,000 - 10,000 


P1 vWF + 

CD31 + 


> 15 11 



C-12202 C-22010 

C-22011 

5,000 - 10,000 


P1 vWF + 

CD31 + 


> 15 12 

Human Aortic 

Endothelial Cells (HAoEC) 

C-12271 C-22020 

C-22022 

5,000 - 10,000 


P2 vWF + 

CD31 + 


> 15 13 



C-12221 C-22020 

C-22022 

5,000 - 10,000 


P2 vWF + 

CD31 + 


> 15 13 



C-12241 C-22010 

C-22011 

5,000 - 10,000 


P2 vWF + 

CD31 + 


> 15 14 



C-12231 C-22010 

C-22011 

5,000 - 10,000 


P2 vWF + 

CD31 + 


> 15 14 




C-12210 C-22020 

C-22022 

10,000 - 20,000 


P2 vWF + 

CD31 + 


> 15 15 



adult donor 

C-12212 C-22020 

C-22022 

10,000 - 20,000 


P2 vWF + 

CD31 + 


> 15 15 



pre-screened 

C-12215 C-22020 

C-22022 

10,000 - 20,000 


P2 vWF + 

CD31 + 


> 15 15 

Human Dermal Blood 

Endothelial Cells (HDBEC) 


C-12211 C-22020 10,000 - 20,000 


P2 Podoplanin - 

Prox-1 - 

CD31 + 

> 15 16 


Endothelial Cells (HDBEC) 

adult donor 

C-12225 C-22020 10,000 - 20,000 


P2 Podoplanin - 

Prox-1 - 

CD31 + 

> 15 16 




C-12216 C-22022 10,000 - 20,000 


P2 Podoplanin + 

Prox-1 + 

CD31 + 

> 15 17 



adult donor 

C-12217 C-22022 10,000 - 20,000 


P2 Podoplanin + 

Prox-1 + 

CD31 + 

> 15 17 



C-12291 C-22020 

C-22022 

10,000 - 20,000 


P2 vWF + 

CD31 + 


> 15 18 



C-12285 C-22020 

C-22022 

10,000 - 20,000 


P2 vWF + 

CD31 + 


> 15 18 

* The media in this table are ready-to-use. For Media Kits see chapter 4.


Product 

Cat. No. 


Recommended 



Passage after 

Thawing 

Marker 

Population 

Doublings 

Page 

Human Pulmonary 

Microvascular 




C-12281 C-22020 

C-22022 

C-12295 C-22020 

C-22022 

10,000 - 20,000 


10,000 - 20,000 


P2 vWF + 

CD31 + 


P2 vWF + 

CD31 + 


> 15 19 

> 15 19 



Human Brain Microvascular 

Endothelial Cells (HBMEC) 

C-12287 10,000 - 20,000 


> 10 20 

P2 vWF + 


CD31 + 

Human Nasal 

Epithelial Cells (HNEpC) 

C-12620 C-21060 10,000 - 15,000 


P2 Cytokeratin + > 15 21 

Human Tracheal 

Epithelial Cells (HTEpC) 

C-12644 C-21060 10,000 - 15,000 



Human Bronchial 

Epithelial Cells (HBEpC) 

C-12640 C-21060 10,000 - 15,000 



Human Small Airway 

Epithelial Cells (HSAEpC) 

C-12642 C-21070 10,000 - 15,000 



Human Mammary 

Epithelial Cells (HMEpC) 

C-12650 C-21010 10,000 - 15,000 



Human Renal 

Epithelial Cells (HREpC) 

C-12665 C-26001 

C-26030 

10,000 - 15,000 



Human Renal Cortical 

Epithelial Cells (HRCEpC) 

C-12660 C-26001 

C-26030 

10,000 - 15,000 



Human Placental 

Epithelial Cells (HPlEpC) 

C-12395 C-26040 10,000 - 15,000 



Normal Human Dermal 

Fibroblasts (NHDF) 



Fibroblasts (NHDF) 

adult donor 

Human Pulmonary 

Fibroblasts (HPF) 

C-12300 C-23010 3,500 - 7,000 


C-12302 C-23020 3,500 - 7,000 


C-12360 C-23020 3,500 - 7,000 


P2 CD90 + > 15 25 

P2 CD90 + > 15 25 

P2 CD90 + > 15 26 



C-12380 C-23020 3,500 - 7,000 


P2 CD90 + > 10 26 

Human Cardiac 

Fibroblasts (HCF) 

C-12375 C-23025 3,500 - 7,000 


P2 CD90 + > 15 26 

Human Uterine 

Fibroblasts (HUF) 

C-12385 C-23020 3,500 - 7,000 


P2 CD90 + > 15 27 



C-12390 C-23020 3,500 - 7,000 


P2 CD90 + > 15 27 

Human Follicle Dermal 

Papilla Cells (HFDPC) 

C-12071 C-26501 5,000 - 10,000 


P2 

Alkaline 

> 10 28 

phosphatase + 

Human Hepatocytes (HHpC) C-12850 C-25010 

C-25020 

* The media in this table are ready-to-use. For Media Kits see chapter 4. 

150,000 


n/a 29 

P0 Phase I reaction + 

Albumin + 

Phase II reaction +

42 




Product 


Keratinocytes (NHEK) 

juvenile foreskin, single donor 



juvenile foreskin, pooled 



adult donor, single donor 



adult donor, pooled 


Melanocytes (NHEM) 




juvenile foreskin, 

cultured in M2 Medium 



adult donor, 

cultured in M2 Medium 

C-12001 C-20011 5,000 


C-12005 C-20011 5,000 


C-12003 C-20011 5,000 


C-12006 C-20011 5,000 


C-12400 C-24010 5,000 - 10,000 


C-12402 C-24300 5,000 - 10,000 


C-12403 C-24300 5,000 - 10,000 


Human Osteoblasts (HOB) C-12720 C-27001 

C-27020 

Human White Preadipocytes 

(HWP) subcutaneous 


(HWP) visceral 

Human Skeletal Muscle Cells 

(SkMC) 

Human Aortic 

Smooth Muscle Cells 

(HAoSMC) 



(HCASMC) 



(HPASMC) 



(HUASMC) 


Smooth Muscle Cells (HTSMC) 


Smooth Muscle Cells (HBSMC) 

Human Bladder 


(HBdSMC) 

Human Prostate 

Smooth Muscle Cells (HPrSMC) 

Human Uterine 


(HUtSMC) 

Cat. No. 


Recommended 


C-12730 C-27410 

C-27436 

C-27438 

C-12732 C-27410 

C-27436 

C-27438 

C-12530 C-23060 

C-23061 


10,000 - 20,000 


5,000 


5,000 


3,500 - 7,000 


C-12533 C-22062 7,500 - 10,000 


C-12511 C-22062 7,500 - 10,000 


C-12521 C-22062 7,500 - 10,000 


C-12500 C-22062 7,500 - 10,000 


C-12565 C-22062 7,500 - 10,000 


C-12561 C-22062 7,500 - 10,000 


C-12571 C-22062 7,500 - 10,000 


C-12573 C-22062 7,500 - 10,000 


C-12575 C-22062 7,500 - 10,000 


Passage after 

Thawing 





P2 Mel-5 + > 15 31 

P2 Mel-5 + > 15 31 

P2 Mel-5 + > 15 31 

P2 

P2 

P2 

Alkaline 

phosphatase + 

Mineralization tested 

Differentiation 

tested 


tested 

> 10 32 

> 10 33 

> 10 33 

P2 Sarcomeric myosin + 


tested 

> 15 34 

P2 Smooth muscle > 15 35 

α-actin + 

P2 

P2 

P2 

P2 

P2 

P2 

P2 

P2 

Marker 

Population 

Doublings 

Page 

Smooth muscle > 15 36 

α-actin + 


α-actin + 


α-actin + 

Smooth muscle 

α-actin + > 15 37 


α-actin + 


α-actin + 

Smooth muscle 

α-actin + > 15 39 


α-actin + 

* The media in this table are ready-to-use. For Media Kits see chapter 4.


Human Primary Cells by Tissue Type 

Product Page Product Page 

• Adipose Tissue 

Human Mesenchymal Stem Cells 

from Adipose Tissue (hMSC-AT) 

47 

• Brain 

Human Brain Microvascular Cells (HBMEC) 

20 




subcutaneous 


visceral 

• Bladder 

33 

33 

• Heart 

Human Cardiac Fibroblasts (HCF) 26 

Human Cardiac Myocytes (HCM) 9 



18 



18 

Human Bladder 

Smooth Muscle Cells (HBdSMC 

38 

• Kidney 

• Blood/Bone Marrow 

Human Renal Epithelial Cells (HREpC) 23 


from Bone Marrow (hMSC-BM) 

47 


(HRCEpC) 

24 

Human Mononuclear Cells from Peripheral 

Blood (hMNC-PB) single donor, ultra-pure 

Human Mononuclear Cells from Peripheral 

Blood (hMNC-PB) pooled, ultra-pure 

Human Mononuclear Cells from Cord 

Blood (hMNC-CB) single donor, ultra-pure 

Human Mononuclear Cells from 

Cord Blood (hMNC-CB) pooled, ultra-pure 

Human CD34 + Progenitor Cells from 

Cord Blood (hCD34 + -CB) single donor 


Cord Blood (hCD34 + -CB) pooled 


Cord Blood (hCD133 + -CB) single donor 


Cord Blood (hCD133 + -CB) pooled 

Human CD14 + Monocytes from Peripheral 

Blood (hMoCD14 + -PB), single donor 


Blood (hMoCD14 + -PB), single donor, 

pre-screened 

Human Monocytes from Peripheral Blood 

(hMo-PB), single donor 


(hMo-PB), single donor, pre-screened 

• Bone/Joint/Skeletal System 

54 

54 

54 

54 

50 

50 

51 

51 

52 

52 

52 

52 

• Large Vessels 


26 


Human Aortic Endothelial Cells (HAoEC) 13 

Human Aortic 

Smooth Muscle Cells (HAoSMC) 




Smooth Muscle Cells (HCASMC) 




Smooth Muscle Cells (HPASMC) 




Smooth Muscle Cells (HUASMC) 


Endothelial Cells (HUVEC) single donor 


Endothelial Cells (HUVEC) pooled 


Endothelial Cells (HUVEC) pre-screened 

35 

13 

36 

14 

36 

12 

37 

11 

11 

11 

Human Osteoblasts (HOB) 32 



14 

Human Chondrocytes (HCH) 10 

Human Skeletal Muscle Cells (SkMC) 34

44 


Product Page Product Page 



• Liver 

Human Hepatocytes (HHpC) 29 

• Respiratory System 

Human Nasal Epithelial Cells (HNEpC) 21 

• Mammary Tissue 

Human Tracheal Epithelial Cells (HTEpC) 21 

Human Mammary 

Epithelial Cells (HMEpC) 

23 


Smooth Muscle Cells (HTSMC) 

37 

• Umbilical Cord 




Smooth Muscle Cells (HUASMC) 


Endothelial Cells (HUVEC) single donor 


Endothelial Cells (HUVEC) pooled 


Endothelial Cells (HUVEC) pre-screened 


from Umbilical Cord Matrix (hMSC-UC) 

• Uterus 

Human Uterine Fibroblasts (HUF) 27 



Human Uterine 

Smooth Muscle Cells (HUtSMC) 

• Placenta 

Human Pericytes from Placenta (hPC-PL) 49 

Human Placental Epithelial Cells (HPlEpC) 24 



• Prostate 

Human Prostate 

Smooth Muscle Cells (HPrSMC) 

12 

37 

11 

11 

11 

47 

19 

39 

27 

39 

• Skin 

Human Bronchial Epithelial Cells (HBEpC) 22 


Smooth Muscle Cells (HBSMC) 

Human Small Airway 

Epithelial Cells (HSAEpC) 

Human Pulmonary Fibroblasts (HPF) 26 




Endothelial Cells (HDMEC) juvenile foreskin 


Endothelial Cells (HDMEC) adult donor 


Endothelial Cells (HDMEC) pre-screened 


Endothelial Cells (HDBEC) juvenile foreskin 


Endothelial Cells (HDBEC) adult donor 


Endothelial Cells (HDLEC) juvenile foreskin 


Endothelial Cells (HDLEC) adult donor 


Fibroblasts (NHDF) juvenile foreskin 


Fibroblasts (NHDF) adult donor 

Human Follicle Dermal 

Papilla Cells (HFDPC) 


(NHEK) juvenile foreskin, single donor 


(NHEK) juvenile foreskin, pooled 


(NHEK) adult, single donor 


(NHEK) adult, pooled 

38 

22 

19 

15 

15 

15 

16 

16 

17 

17 

25 

25 

28 

30 

30 

30 

30 


Melanocytes (NHEM) juvenile foreskin 

Normal Human Epidermal Melanocytes (NHEM) 

juvenile foreskin, cultured in M2 Medium 

Normal Human Epidermal Melanocytes (NHEM) 

adult donor, cultured in M2 Medium 

31 

31 

31


2 

PromoCell offers a collection of adult stem cells, as well as 

differentiated blood cells from normal human bone marrow, 

umbilical cord tissue, placenta, adipose tissue, peripheral blood, 

and cord blood. All cells are obtained from approved donor programs 

at associated medical centers. High-quality media that 

simulate the in vivo environment as well as optimized differentiation 

media systems are also available for these cell types.

46 


2 


2.1 Mesenchymal Stem Cells ............. 47 

2.2 Pericytes .......................... 49 

2.3 CD34 + Progenitor Cells ............... 50 

2.4 CD133 + Progenitor Cells .............. 51 

2.5 

new 

Monocytes ...................... 52 

2.6 Mononuclear Cells new ................ 54 

Overview Human Stem and Blood Cells. ....... 56

2 Human Stem and Blood Cells 47 

2.1 Mesenchymal Stem Cells 

Applications 




Mesenchymal Stem Cell 

Stem cell research 

Growth Medium (see page 86) 

Regenerative medicine research 

PromoCell hMSC-BM are harvested from 


Tissue engineering studies 

normal human bone marrow. The cells 

Growth Medium DXF (see page 86) 

Developmental experiments 

are tested for their ability to differentiate 

Mesenchymal Stem Cell Adipogenic 

Features 

in vitro into adipocytes, chondrocytes, 

osteoblasts, and neuronal cells. 

Differentiation Medium (see page 86) 

Mesenchymal Stem Cell Chondrogenic 


Multipotent adult stem cells 

Tested for in vitro differentiation 

Routinely analyzed by flow cytometry 

Growth Medium and five optimized 

Differentiation Media available 


from Umbilical Cord Matrix 

(hMSC-UC) 

PromoCell hMSC-UC are harvested from 


Differentiation Medium w/o Inducers 

(see page 86) 

Mesenchymal Stem Cell Osteogenic 


Mesenchymal Stem Cell Neurogenic 

2 Human Stem 

and Blood Cells 

Mesenchymal Stem Cells (MSC), also 

normal human umbilical cord matrix 


termed Mesenchymal Stromal Cells, are 

(Wharton’s jelly). The cells are tested for 


multipotent cells that can differentiate 

their ability to differentiate in vitro into 

Cell Dissociation Solution ACF 

into a variety of cell types and have the 

adipocytes, chondrocytes, osteoblasts, 


capacity for self renewal. MSC have 

and neuronal cells. 

Fibronectin Solution, human 

been shown to differentiate in vitro or 


in vivo into adipocytes, chondrocytes, 

osteoblasts, myocytes, neurons, hepatocytes, 

and pancreatic islet cells. Opti- 




MSC-Qualified Fetal Calf Serum 

mized PromoCell media are available to 

(see page 93) 

support both the growth of MSC and 

PromoCell hMSC-AT are harvested from 

hMSC-BM Pellet (see page 98) 

their differentiation into several differ- 

normal human adipose tissue. The cells 

hMSC-UC Pellet (see page 98) 

ent lineages. Recent experiments sug- 

are tested for their ability to differentiate 

hMSC-AT Pellet (see page 98) 

gest that differentiation capabilities into 

in vitro into adipocytes, chondrocytes, 

diverse cell types vary between MSC of 

osteoblasts, and neuronal cells. 

different origin. 



Fig. 1: Undifferentiated hMSC-BM cul- 

Fig. 2: Undifferentiated hMSC-BM cul- 

tured in MSC Growth Medium. 

tured in MSC Growth Medium DXF.

48 

2 Human Stem and Blood Cells 

2 Human Stem 


Fig. 3.1 Fig. 3.2 

Fig. 3.1: hMSC-BM after in vitro differentiation 

into adipocytes using PromoCell 


Differentiation Medium (lipid droplets 

stained with Oil Red O). 

Fig. 3.2: hMSC-BM spheroid after in vitro 

differentiation into cartilage using PromoCell 


Differentiation Medium (Alcian Blue 

staining). 


into osteoblasts using PromoCell 

Mesenchymal StemCell Osteogenic Differentiation 

Medium (Alizarin Red S staining). 


into neuronal cells using PromoCell 

Mesenchymal Stem Cell Neurogenic Differentiation 

Medium. 

Fig. 3.3 Fig. 3.4 







C-12974 

C-12975 

hMSC-BM Pellet 1x10 6 cells in RNAlater ® C-14090 


from Umbilical Cord Matrix (hMSC-UC) 



C-12971 

C-12972 

hMSC-UC Pellet 1x10 6 cells in RNAlater ® C-14091 





C-12977 

C-12978 

hMSC-AT Pellet 1x10 6 cells in RNAlater ® C-14092


2.2 Pericytes 

Applications 

blood vessel walls. They are important 

for angiogenesis, the structural integ- 


rity of the microvasculature, and blood 

Regenerative medicine research 

flow regulation. However, they can also 

Oncology 

develop into malignant tumors called 

hemangiopericytomas. 

Features 

To date, pericytes have demonstrated 

Non-hematopoietic multipotent 

the ability to differentiate into adipo- 

mesenchymal-like cells 

Important in angiogenesis 

Routinely characterized by flow 

cytometry 

Isolated from human placenta 

cytes, osteoblasts, fibroblasts, smooth 

muscle cells, and phagocytes (macrophages). 


Pericyte Growth Medium 

Fig. 4: Proliferating human pericytes 

(hPC-PL) from the placenta (phase contrast). 

2 Human Stem 


Pericytes are multipotent mesenchymallike 

cells found in association with small 

(see page 87) 



hPC-PL Pellet (see page 98) 

Count 

Fig. 5: Flow cytometric analysis of PromoCell Pericytes. 

> 98% of the cells are CD146 + . 

50 

25 

0 

CD146 – CD146 + 

10 0 10 1 10 2 10 3 10 4 

CD146 




Human Pericytes from Placenta (hPC-PL) 



C-12980 

C-12981 

hPC-PL Pellet 1x10 6 cells in RNAlater ® C-14095

50 


2.3 CD34 + Progenitor Cells 

Applications 

marker for primitive blood- and bone 


marrow-derived progenitor cells, espe- 

Hematopoietic Progenitor Cell 


cially for hematopoietic and endothelial 

Expansion Medium DXF 

Transplantation basic research 

stem cells. Although the biological func- 

(see page 87) 

Hematological and immunological 

tions of CD34 are largely unknown, re- 

Hematopoietic Progenitor Medium 

studies 

cent data suggest that CD34 is involved 

(see page 87) 

Vascular and angiogenesis research 

in maintenance of the progenitor cells in 

Endothelial Progenitor Medium 

Oncology research 

a phenotypically undifferentiated state. 

(see page 88) 

PromoCell offers CD34 + Progenitor Cells 

Features 

from the cord blood of healthy donors. 


The CD34 + Progenitor Cells contain 

G-CSF (see page 106) 

2 Human Stem 


Multipotent hematopoietic/ 

endothelial progenitors 

CD34 + cell purity > 95% 

Superior viability 

two main cellular subpopulations, hematopoietic 

and endothelial progenitor 

cells. Therefore, CD34 + Progenitor Cells 

are suitable for a series of studies, e.g. 

SCF (see page 106) 

flt-3 ligand (see page 106) 

IL-3 CC (see page 106) 


directed differentiation into more com- 

TPO (see page 106) 

CD34 is a glycosylated transmembrane 

mitted types of blood cells and endothe- 

CD34 antibody 

protein and represents a well-known 

lial lineages. 


Count 

Fig. 6: Flow cytometric analysis of PromoCell CD34 + 

Progenitor Cells. > 95% of the cells are CD34 + . 

50 

25 

0 

0 

CD34 – CD34 + 

10 10 2 10 3 10 4 


CD34 



Human CD34 + Progenitor Cells from Cord 

Blood (hCD34 + -CB), single donor 

Human CD34 + Progenitor Cells 

from Cord Blood (hCD34 + -CB), pooled 

100,000 cryopreserved cells C-12921 

100,000 cryopreserved cells C-12924


2.4 CD133 + Progenitor Cells 

Applications 

CD133 (AC133, Prominin-1) is a glyco- 


protein with sub-cellular localization in 


Hematology research 

plasma membrane protrusions. In hu- 

Expansion Medium DXF (see page 87) 


mans, this protein was originally identi- 

Cytokine Mix E for HPC Expansion 

Oncology 

fied as an antigenic marker expressed 

Medium DXF (see page 87) 

Features 

on hematopoietic stem cells. In addition, 

CD133 is also expressed by neural and 


(see page 87) 

muscle progenitor cells. 

Primitive hematopoietic progenitors 


Available from cord blood 

CD133 + cells are isolated from fresh cord 


CD133 + cell purity > 95% 

blood. These cells are highly prolifera- 

SCF (see page 106) 

Superior viability 

Expansion and Differentiation Media 

available 

tive primitive hematopoietic progenitors. 

They can give rise to mature terminally 

differentiated hematopoietic cells 

by means of directed differentiation. 

flt-3 ligand (see page 106) 



TPO (see page 106) 

2 Human Stem 


PromoCell also provides suitable expan- 

CD133 antibody 

sion and differentiation media. 


CD45 

Fig. 7: Flow cytometric analysis of cord blood-derived 

CD133 + Progenitor Cells, demonstrating their high 

purity. 

10 3 

10 2 

10 

0 

CD133 + cells 

0 10 10 2 10 3 


CD133 




Cord Blood (hCD133 + -CB), single donor 


Cord Blood (hCD133 + -CB), pooled 

100,000 cryopreserved cells C-12927 

100,000 cryopreserved cells C-12929

52 


2.5 Monocytes 

Applications 

gradient-based method completely 


devoid of using any immunomagnetic 

Mononuclear Cell Medium (see page 89) 

Dendritic Cell Research 

beads. The advantage of these com- 

DC Generation Medium (see page 88) 

(Auto-)Immunity 

pletely native Monocytes is that poten- 

Infection biology 

tial effects of bound magnetic particles 


Features 

(which can be ingested by this phagocytic 

cell type) are excluded. 

hMoCD14 + -PB single donor Pellet 

(see page 98) 

hMoCD14 + -PB single donor 

Isolated from fresh peripheral blood 


CD14 + -selected as well as “untouched“ 

Human CD14 + Monocytes 

(hMoCD14 + -PB) 

pre-screened Pellet (see page 98) 

hMo-PB single donor Pellet 

(see page 98) 

2 Human Stem 


Monocytes available 

Monocytes (Mo) are immature phagocytic 

cells circulating in the blood. Acting 

PromoCell Human CD14 + Monocytes 

are isolated from fresh peripheral blood. 

After isolation of the ultra-pure mono- 

hMo-PB single donor 

pre-screened Pellet (see page 98) 

PromoFectin-Macrophage 

Transfection Reagent 

as antigen-presenting immune cells, they 

nuclear cell fraction by proprietary meth- 


can phagocytize and degrade microbes 

ods, the CD14 + Monocytes are isolated 


and particulate matter. Monocytes can 

by positive selection with > 95% purity. 

GM-CSF (see page 106) 

differentiate into several different cell 


types. The most common applications 

In addition, hMoCD14 + -PB are available 

TNF-alpha (see page 106) 

for Monocytes are the in vitro differen- 

which are pre-screened for their differ- 

M-CSF (see page 106) 

tiation into monocyte-derived dendritic 

entiation capacity into dendritic cells. 

sRANKL (see page 106) 

cells (moDC), macrophages, and osteo- 

CD14 antibody 

clasts as well as their use as phagocytic 

immune cells in immunology and infec- 

Human Monocytes (hMo-PB) 


tion biology. 

PromoCell Human “untouched” Mono- 

CD14 belongs to the family of LPS re- 

cytes are isolated from fresh peripheral 

ceptor antigens and is strongly expressed 

blood. After isolation of the ultra-pure 

on the majority of Monocytes. Thus, 

mononuclear cell fraction by proprietary 

binding of immunomagnetic particles 

methods, the Monocyte fraction is fur- 

to this antigen is employed for purifica- 

ther purified to > 70% by an advanced 

tion of PromoCell CD14 + -Monocytes 

multi-gradient technique. 

(hMoCD14 + -PB) from Mononuclear 

Cells (MNC). 

In contrast, the “untouched” Monocytes 

(hMo-PB) provided by PromoCell 

In addition, hMo-PB are available which 

are pre-screened for their differentiation 

capacity into dendritic cells. 

Fig. 8: Cluster formation during 

differentiation of Monocytes into moDC. 

are enriched from MNC by a density 



Fig. 9: Mature moDC cultured in 

PromoCell DC Generation Medium showing 

typical dendrite-like structures.


Count 

Fig. 10a: Flow cytometric analysis of day 8 mature 

moDCs generated in the PromoCell DC Generation 

Medium. More than 98% of the cells are negative for 

CD14. 

12,5 

0 

0 

CD14 - 

CD14 + 

10 1 10 2 10 3 10 4 

CD14 


2 Human Stem 


Count 

Fig. 10b: Flow cytometric analysis of day 8 mature 

moDCs generated in the PromoCell DC Generation 

Medium. The DC maturation marker CD83 is strongly 

expressed by > 98% of the cells. 

12,5 

0 

0 

CD83 - 

CD83 + 

10 1 10 2 10 3 10 4 

CD83 





Blood (hMoCD14 + -PB), single donor 

10 x 10 6 new 

cryopreserved cells C-12909 

hMoCD14 + -PB single donor Pellet 1x10 6 cells in RNAlater ® new C-14110 


Blood (hMoCD14 + -PB), single donor, 

pre-screened 


pre-screened Pellet 

10 x 10 6 new 


1x10 6 cells in RNAlater ® new 

C-14112 


10 x 10 6 cryopreserved cells 

new 

C-12910 

(hMo-PB), single donor 

hMo-PB single donor Pellet 1x10 6 cells in RNAlater ® new C-14111 


10 x 10 6 cryopreserved cells 

new 

C-12913 

(hMo-PB), single donor, pre-screened 

hMo-PB single donor pre-screened Pellet 1x10 6 cells in RNAlater ® new 

C-14113

54 


2.6 Mononuclear Cells 

Applications 

PromoCell Mononuclear Cells (MNC) 

represent the lymphocyte and monocyte 

tors, monocytes, and lymphocyte subpopulations 

(see. Fig. 12). 

fraction isolated from umbilical cord blood 


Transplantation basic research 

or peripheral blood. They are prepared 

by optimized low-density gradient cen- 


Peripheral Blood (hMNC-PB) 

Hematological and immunological 

trifugation, which effectively reduces the 

studies 

granulocyte content (see Fig. 11). Red 

Adult peripheral blood contains large 

Oncology research 

blood cells are gently but thoroughly de- 

numbers of mature immune cells but also 

Virology and infection biology 

pleted by proprietary techniques instead 

some progenitor cells. Typical application 

Features 

of osmotic lysis. This results in ultra-pure 

mononuclear cells with superior viability 

fields are immunology, oncology, hematology, 

virology, and infection biology. 

and unchanged biological function. In 

2 Human Stem 



Granulocyte content < 5% 

Red blood cells depleted without lysis 

High purity and viability 

addition, the cells do not clump after 

thawing, allowing for easy handling and 

reproducible results. 

The MNC can be used directly for ex- 


Cord Blood (hMNC-CB) 

hMNC-CB are isolated from umbilical 

Available from single and pooled 

periments or as a starting material for 

cord blood, which has a much higher 

donors 

the isolation of several cell types, e.g., 

content of primitive progenitor cells com- 

hematopoietic and endothelial progeni- 

pared to adult peripheral blood. 

Size [µm] 

Granulocytes 

Monocytes 

Fig. 11: Flow cytometric analysis of Mononuclear 

Cells from Cord Blood (hMNC-CB). Granulocyte 

content 1%. 

16 

14 

12 

10 

8 

0 

Lymphocytes 


0 10 10 2 10 3 

10 4 

Side Scatter 

CD45 

10 4 

CD34 + cells 

Fig. 12: Flow cytometric determination of CD34 + cells 

within the PromoCell Mononuclear Cells from Cord 

Blood (hMNC-CB). 

10 3 

10 2 

10 

0 


0 10 10 2 10 3 10 4 

CD34



Mononuclear Cell Medium 

(see page 89) 

Hematopoietic Progenitor Medium* 

(see page 87) 

Endothelial Progenitor Medium* 

(see page 88) 


Expansion Medium DXF (see page 87) 

Cytokine Mix E for HPC Expansion 

Medium DXF (see page 87) 


hMNC-PB single donor Pellet 

(see page 97) 

hMNC-CB single donor Pellet 

(see page 97) 

hMNC-PB pooled Pellet 

(see page 97) 

hMNC-CB pooled Pellet 

(see page 97) 

Lymphocyte Separation 


GM-CSF (see page 106) 


M-CSF (see page 106) 

TNF-alpha (see page 106) 

sRANKL (see page 106) 


2 Human Stem 



Human Mononuclear Cells 

from Peripheral Blood (hMNC-PB), 

single donor, ultra-pure 

25 x 10 6 cryopreserved cells C-12907 

hMNC-PB single donor Pellet 1x10 6 cells in RNAlater ® C-14098 


from Peripheral Blood (hMNC-PB), 

pooled, ultra-pure 

25 x 10 6 new 


hMNC-PB pooled Pellet 1x10 6 cells in RNAlater ® new 

C-14099 


from Cord Blood (hMNC-CB), 



hMNC-CB single donor Pellet 1x10 6 cells in RNAlater ® C-14096 





hMNC-CB pooled Pellet 1x10 6 cells in RNAlater ® C-14097 

* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or “Hill-assays” for endothelial progenitor cells 

(Hill et al. 2003, N Engl J Med 348: 593-600).

56 


Overview Human Stem and Blood Cells 

Product 

Cat. No. 

Cryopr. 


Recommended 

Culture Media 

Recommended 


Media 

Recommended 

Sera 

Plating 

Density 

Page 

Human Mesenchymal Stem 

Cells from Bone Marrow 

(hMSC-BM) 

C-12974 C-28010 

C-28019 

C-28011 

C-28012 

C-28013 

C-28014 

C-28015 

C-37385 

C-37386 

4,000 cells 47 

per cm 2 

2 Human Stem 



Cells from Umbilical Cord 

Matrix (hMSC-UC) 


Cells from Adipose Tissue 

(hMSC-AT) 

C-12971 C-28010 

C-28019 

C-12977 C-28010 

C-28019 

C-28011 

C-28012 

C-28013 

C-28014 

C-28015 

C-28011 

C-28012 

C-28013 

C-28014 

C-28015 

C-37385 

C-37386 

C-37385 

C-37386 

4,000 cells 47 

per cm 2 

4,000 cells 47 

per cm 2 

Human Pericytes 

from Placenta (hPC-PL) 

C-12980 C-28040 C-37385 

C-37386 

4,000 cells 49 

per cm 2 


from Cord Blood (hCD34 + -CB), 

single donor 

C-12921 C-28021 

C-39890 

C-28020* 

C-28025* 

C-37387 

C-37388 

5,000 cells 

per ml 

50 



pooled 

C-12924 C-28021 

C-39890 

C-28020* 

C-28025* 

C-37387 

C-37388 

5,000 cells 

per ml 

50 



single donor 

C-12927 C-28021 

C-39890 

C-28020* C-37387 

C-37388 

5,000 cells 

per ml 

51 


from Cord Blood (hCD133 + - 

CB), pooled 

C-12929 C-28021 

C-39890 

C-28020* C-37387 

C-37388 

5,000 cells 

per ml 

51 


from Peripheral Blood 

(hMoCD14 + -PB), single donor 

C-12909 C-28030 C-37387 

C-37388 

1 x10 6 cells 

per ml 

52 


from Peripheral Blood 

(hMoCD14 + -PB), single donor, 

pre-screened 

C-12912 C-28030 C-28050 C-37387 

C-37388 

1 x10 6 cells 

per ml 

52 

Human Monocytes from 

Peripheral Blood (hMo-PB), 

single donor 

C-12910 C-28030 C-37387 

C-37388 

1 x10 6 cells 

per ml 

52 

Human Monocytes from 

Peripheral Blood (hMo-PB), 

pre-screened 

C-12913 C-28030 C-28050 C-37387 

C-37388 

1 x10 6 cells 

per ml 

52 


from Peripheral Blood (hMNC- 

PB), single donor, ultra-pure 

C-12907 C-28030 C-37387 

C-37388 

1 x10 6 cells 

per ml 

54 


from Peripheral Blood (hMNC- 

PB), pooled, ultra-pure 

C-12908 C-28030 C-37387 

C-37388 

1 x10 6 cells 

per ml 

54 




C-12901 C-28030 

C-28021 

C-39890 

C-28020* 

C-28025* 

C-37387 

C-37388 

1 x10 6 cells 

per ml 

54 




C-12904 C-28030 

C-28021 

C-39890 

C-28020* 

C-28025* 

C-37387 

C-37388 

1 x10 6 cells 

per ml 

54 

* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or “Hill-assays” for endothelial 

progenitor cells (Hill et al. 2003, N Engl J Med 348: 593-600).


3 

PromoCell offers a set of ready-to-use Cell Model Systems, 

which include primary cells and optimized media. In vivo 

situations are very closely mimicked by these model systems 

because of the spatial arrangement of the cultured cells, and 

they allow easy monitoring of complex cell growth processes 

and fast screening of test substances.

58 


3 


3.1 Angiogenesis Assays and Kits .......... 59

3 Cell Model Systems 59 

3.1 Angiogenesis Assays and Kits 

Applications 

controlled by pro-angiogenic and antiangiogenic 

factors: 



Proteolytic activity 


Angiogenic growth factors activate 

Oncology investigations 

receptors on endothelial cells in pre- 

Chemokine/Chemotaxis assays 

existing blood vessels. The activated en- 

Drug screening 

dothelial cells begin to release proteases 

that degrade the basement membrane. 

Features 

Migration 

Four different assays available 

Endothelial cells from the parent vessel 

Available with HUVEC or HDMEC 

walls migrate through the gaps in the 

Fig. 1: Vessel network with newly 

Ready to use 

basement membrane into the surround- 

formed capillaries. 

Fast results 

ing tissue. 

Easy monitoring 

Proliferation 

PromoCell offers four diverse angiogen- 

Angiogenesis, the growth of new blood 

The migrated endothelial cells proliferate 

esis models to simulate these angiogen- 

vessels from pre-existing ones, is a normal 

process in growth and development, 

as well as in wound healing. However, 

it is also vitally involved in a number of 

and form solid sprouts. These sprouts 

extend toward the source of the angiogenic 

stimulus. 

esis stages or the whole process in vitro, 

thus making pro- or anti-angiogenic 

testing fast and efficient: 

3 Cell Model 

Systems 

pathological processes like tumor growth 

Lumen formation 

3D-Angiogenesis Assay 

and metastasis, rheumatoid arthritis, and 

The cells in the solid sprouts form a lu- 

Planar Migration Assay 

macular degeneration. It occurs in four 

men, which results in a fully developed 

Transmigration Kit 

well-characterized stages, which are 

vessel. 

Invasion Kit 

3D-Angiogenesis Assay 

within two days and can be quantified 

without cell staining (see Fig. 2). 

The PromoCell ready-to-use 3D-An- 

The number and length of the sprouts 

giogenesis Assay allows fast and easy 

correspond to the pro- or anti-angioge- 

screening for pro-angiogenic and anti- 

nicity of the test substance. 

angiogenic effects of test substances in 

Since only one cell type is used in the as- 

a convenient 24-well format. 

say, unwanted side effects of co-cultured 

The assay simulates the entire angiogenesis 

process in vitro - proteolytic 

cells are excluded. 

100 μM 

activity, migration, proliferation, and lu- 

Substances that have already been 

men formation - all in three dimensions. 

analyzed using the 3D-Angiogene- 

Therefore, the migration of tip cells 

sis Assay: VEGF (see Fig. 2 and 3), 

or the formation of three dimensional 

FGF-1, FGF-2, and kinase inhibitors 

networks can also be monitored. This is 

e.g. SU5402, SU5614 (see Fig. 4), 

clearly an advantage over other angio- 

SU6656. 

genesis assays like the tube formation 

assay. 

The assay utilizes endothelial cell 

spheroids embedded in a collagen ma- 

100 μM 

trix, which very closely resembles the in 

Fig. 2: Endothelial cell spheroids 48 

vivo basement membrane environment. 

hours after stimulation with 20 ng/ml 

After successful stimulation, new blood 

VEGF* (top) and unstimulated negative 

vessels sprout into the collagen matrix 

control (bottom). 

* VEGF ist provided as a positive control with the Assay.

60 

3 Cell Model Systems 

Cumulative sprout length [μm] 

1400 

1200 

1000 

800 

600 

400 

Fig. 3: Rising VEGF concentrations lead to increased 

cumulative sprout length. 

200 

0 

0 

0.78 1.56 3.12 6.25 12.5 25 

VEGF [ng/ml] 


Systems 

Cumulative sprout length [μm] 

1400 

1200 

1000 

Fig. 4: Increasing concentrations of the kinase inhibitor 

SU5614 added to VEGF [25 ng/ml] stimulated spheroids. 

Rising SU5614 concentrations lead to decreased 

cumulative sprout length (Control: no VEGF and no 

SU5614 added). 

800 

600 

400 

200 

0 

0 1 10 10 2 10 3 10 4 control 

SU5614 [nM] 

Advantages of the PromoCell 

3D-Angiogenesis Assay: 

3D growth of new blood vessels 

Results available in 2 days 

No staining necessary for monitoring 

Automated analysis possible 



Cells pre-screened (see page 11) 


Endothelial Cells pre-screened 

(see page 15) 

FGF-1, FGF-2 (see page 106) 

SU5402 (see www.promokine.info) 



3D-Angiogenesis Assay (HUVEC) 

3D-Angiogenesis Assay (HDMEC) 

8 assays 

2 x 8 assays 

3 x 8 assays 

8 assays 

2 x 8 assays 

3 x 8 assays 

C-13010 

C-13011 

C-13012 

C-13020 

C-13021 

C-13022 

Note: The assays will always be delivered on Wednesdays - please start immediately after arrival.


Planar Migration Assay 

The PromoCell Planar Migration Assay 

allows fast and easy screening for migration 

stimulating effects of test substances 

in vitro. Cells from a confluent 

area migrate into a cell free section at a 

speed which is influenced by the applied 

test substance. After 3-4 days, the distance 

between the two migration fronts 

is measured (cell staining is not necessary). 

This distance is proportional to the 

migration stimulating capacity of the applied 

test substance (see Fig. 5). 

The cell free section is created at 

PromoCell by a special culture technique 

and not by the scraping of cells. This ensures 

undamaged cells at the edge and a 

defined migration front. 

The assay is ready-to-use and consists of 

primary endothelial cells with a defined 

migration front and optimized medium. 

Substances that have already been analyzed 

using the Migration Assay: 

VEGF, FGF-1, FGF-2, kinase inhibitors 

e.g. SU5614. 




2000 µm 1500 µm 

Fig. 5: PromoCell Planar Migration 

Assay without VEGF (left) and with 

25 ng/ml VEGF in the medium (right). 


Systems 

Distance between migration fronts [μm] 

2000 

1500 

1000 

Fig. 6: Comparison of endothelial cell migration with 

and without VEGF using the PromoCell Planar Migration 

Assay. 

500 

0 

0 25 

VEGF [ng/ml] 



Planar Migration Assay (HUVEC) 12 assays C-13030 

Planar Migration Assay (HDMEC) 12 assays C-13031 

Note: The assays will always be delivered on Wednesdays - please start immediately after arrival.

62 

3 Cell Model Systems 

Transmigration Kit 

The PromoCell Transmigration Kit utilizes 

the migration of endothelial cells 

along a cytokine gradient (chemotaxis) 

to measure the chemotactic capability of 

test substances in vitro. The basis for the 

kit are two compartments separated by 

a membrane with an accurately defined 

pore size of 8 μm (see Fig. 7). A potential 

chemotactic factor is placed in one compartment 

and a gradient develops across 

the membrane. Endothelial cells placed 

in the other compartment migrate along 

this gradient. Within four hours after 

adding the test substance, the number 

of cells that have migrated through the 

membrane can easily be counted (see 

Fig. 8). This number is proportional to 

the chemotactic capability of the tested 

substance. The kit is ready-to-use and 

consists of primary endothelial cells (proliferating), 

test chambers, and optimized 

medium. 


using the Transmigration Kit: 


e.g. SU5614. 




1. 2. 3. 4. 


Systems 

Fig. 7: Workflow of the PromoCell Transmigration Kit. 

1. Primary cells are added to the cell culture insert and the test substance is added into the lower compartment. 

2. The cells attach to the membrane of the insert. 

3. The cells migrate into the lower compartment through the pores of the membrane. 

4. After migration, the cells attach to the lower surface of the membrane and can be analyzed. 

Fig. 8: Cells that have migrated 

through the membrane without VEGF 

(left) and with 20 ng/ml VEGF in the 

medium (right). 



Transmigration Kit (HUVEC) 24 assays C-13035 

Transmigration Kit (HDMEC) 24 assays C-13036 

Note: The kits will always be delivered on Wednesdays - please start immediately after arrival.


Invasion Kit 

The PromoCell Invasion Kit simulates the 

proteolytic activity of endothelial cells 

and their directed migration in vitro. In 

this fashion, it is possible to analyze the 

influence of different substances on the 

proteolytic activity and their chemotactic 

efficacy in one assay. 

The basis for the kit are two compartments 

separated by a membrane with 

an accurately defined pore size of 8 μm 

(see Fig. 9). This membrane is covered 

by a matrix, which mimics the basement 

membrane of blood vessels. A potential 

chemotactic factor is placed in one 

compartment and a gradient develops 

across the membrane. Endothelial cells 

placed in the other compartment degrade 

the matrix and then migrate along 

this gradient. Within 24 hours after adding 

the test substance, the number of 

cells, which have migrated through the 

membrane, can easily be counted. This 

number is proportional to the chemotactic 

capability of the tested substance and 

its influence on the proteolytic activity. 

The kit is ready-to-use and consists of 

primary endothelial cells (proliferating), 

coated test chambers, and optimized 

medium. 


using the Invasion Kit: 


e.g. SU5614. 




1. 2. 3. 4. 5. 


Systems 

Fig. 9: Workflow of the PromoCell Invasion Kit. 

1. Primary cells are added to the cell culture insert and the test substance is added to the lower compartment. 

2. The cells attach to the extracellular matrix on the membrane of the insert. 

3. The cells release proteases that degrade the basement membrane. 

4. The cells migrate into the lower compartment through the pores of the membrane. 

5. After migration, the cells attach to the lower surface of the membrane and can be analyzed. 



Invasion Kit (HUVEC) 24 assays C-13040 

Invasion Kit (HDMEC) 24 assays C-13041 

Note: The kits will always be delivered on Wednesdays - please start immediately after arrival.


4 

High-quality media and sera are vital for successful cell culture. 

Therefore, PromoCell adheres to strict quality standards and 

produces media and sera only from raw materials from strictly 

approved sources. We also have invested a lot of effort in media 

optimization and have substituted serum by defined substances 

wherever possible. This ensures effective cell growth 

and avoids the unpredictable effects of indeterminate compounds.

66 


4 


4.1 Media for Primary Cells .............. 67 

4.1.1 Myocyte Medium. .......................68 

4.1.2 Chondrocyte Medium ....................68 

4.1.3 Endothelial Cell Media ....................69 

4.1.4 Endothelial Cell Media MV. ................70 

4.1.5 Epithelial Cell Media. .....................71 

4.1.6 Fibroblast Media new ......................76 

4.1.7 Follicle Dermal Papilla Cell Medium ..........78 

4.1.8 Hepatocyte Media .......................79 

4.1.9 Keratinocyte Medium. ....................80 

4.1.10 Melanocyte Media .......................81 

4.1.11 Osteoblast Media. .......................82 

4.1.12 Preadipocyte/Adipocyte Media .............83 

4.1.13 Skeletal Muscle Cell Media. ................84 

4.1.14 Smooth Muscle Cell Medium ...............85 

4.1.15 Freezing Medium ........................85 

4.2 Media for Stem and Blood Cells. ........ 86 

4.2.1 Mesenchymal Stem Cell Media new ...........86 

4.2.2 Pericyte Medium ........................87 

4.2.3 Hematopoietic Progenitor Cell Media. ........87 

4.2.4 Endothelial Progenitor Cell Medium ..........88 

4.2.5 Dendritic Cell Generation Media new ..........88 

4.2.6 Mononuclear Cell Medium. ................89 

4.2.7 Blood Cell Separation Medium ..............89 

4.3 Classical Media ..................... 90 

4.4 Insect Cell Culture Media ............. 92 

4.5 Sera ............................. 92

4 Media and Sera 67 

4.1 Media for Primary Cells 

The PromoCell media for primary cells 

are formulated for the optimal growth 

of specific types of primary human cells 

and a variety of primary animal cells. 

For most cell types, they are available 

as Medium (Ready-to-use) for maximal 

convenience or as Medium Kit, which 

allows user control over the supplement 

type and concentration. 

Each medium lot is tested for its ability to 

support the growth of the intended primary 

cells. In addition, biochemical and 

sterility tests are performed*. 

Note: The media for primary cells do not 

contain antibiotics. 

Medium (Ready-to-use) 

The Medium (Ready-to-use) consists of 

a 500 ml bottle with Basal Medium and 

one vial with SupplementMix. Adding 

the SupplementMix to the Basal Medium 

results in the complete medium, which 

contains all the growth factors and 

supplements necessary for the propagation 

of the specific human primary cells 

in culture (including L-Glutamine and 

NaHCO 3 

). 

The shelf life of the Basal Medium is 

9 months (at 4 to 8°C), that of the 

SupplementMix is 12 months (at -20°C) 

from the date of manufacture. After 

adding the SupplementMix, the shelf life 

of the complete medium is 6 weeks (at 

4 to 8°C). 

Medium Kit 

The Medium Kit consists of a 500 ml 

bottle with Basal Medium and a SupplementPack. 

The SupplementPack is a set 

of individual vials with pre-measured 

supplements. Adding all components of 

the SupplementPack to the Basal Medium 

results in the complete medium, 

which contains all the growth factors 

and supplements necessary for the propagation 

of specific human primary cells 

in culture (including L-Glutamine and 

NaHCO 3 

). Since all supplements can be 

added separately, the user has full control 

over the media formulation and can 

adapt it to his/her experimental requirements. 


9 months (at 4 to 8°C), and that of 

the SupplementPack is 12 months 

(at -20°C) from the date of manufacture. 

After adding the supplements, 

the shelf life of the complete 

medium is 6 weeks (at 4 to 8°C). 

4 Media and Sera 

Basal Media (with or without phenol 

red) as well as SupplementMixes and 

SupplementPacks can also be purchased 

separately. 

If you require special media modifications, we offer a Custom Media Service starting at 10 bottles per order. 

Please ask for details. 

*Certificates of analysis are available on request.

68 


Low Serum 

4.1.1 Myocyte Medium 

Myocyte Growth Medium 

Recommended for 

Human Cardiac Myocytes 

(see page 9) 

The PromoCell Myocyte Growth Medium 

is a low serum (5% V/V) medium supplemented 

with well chosen hormones and 

growth factors. It was developed for the 

optimal expansion and maintenance of 

primary cardiac myocytes in culture. The 

medium is optimized for primary human 

cells but can also be used for primary porcine 

cardiac myocytes. 


Fetal Calf Serum (see page 92) 

Final supplement concentrations 

(after addition to the medium) 

Fetal Calf Serum 

Epidermal Growth Factor 

(recombinant human) 

Basic Fibroblast Growth Factor 


Insulin (recombinant human) 

0.05 ml / ml 

0.5 ng / ml 

2 ng / ml 

5 µg / ml 



Myocyte Growth Medium (Ready-to-use) 500 ml C-22070 


Myocyte Growth Medium Kit 500 ml C-22170 

Myocyte Basal Medium 500 ml C-22270 

Myocyte Basal Medium, phenol red-free 500 ml C-22275 

Myocyte Growth Medium SupplementMix for 500 ml C-39275 

Myocyte Growth Medium 

for 500 ml C-39270 

SupplementPack 

4.1.2 Chondrocyte Medium 



Human Chondrocytes (see page 10) 

The PromoCell Chondrocyte Growth 

Medium is designed for the optimal 

cultivation of human chondrocytes. The 

fetal calf serum in the medium is strictly 

pretested for optimal performance. 

The medium is optimized for primary 

human cells but can also be used for primary 

porcine and rat chondrocytes. 

Final supplement concentration 



0.1 ml / ml 




(Ready-to-use) 

500 ml C-27101 

Chondrocyte Basal Medium 500 ml C-27111 

Chondrocyte Basal Medium, 

phenol red-free 


SupplementMix 

500 ml C-27115 

for 500 ml C-39635


Low Serum 

4.1.3 Endothelial Cell Media 








Human Pulmonary Artery Endothelial 


Human Saphenous Vein Endothelial 


Endothelial Cell Growth Medium and 

Endothelial Cell Growth Medium 2 are 

low-serum (2% V/V) media optimized 

for the cultivation of endothelial cells 

from large blood vessels. 

Endothelial Cell Growth Medium 2 lacks 

Endothelial Cell Growth Supplement 

(ECGS, bovine hypothalamic extract) 

but contains Insulin-like Growth Factor 

(Long R3 IGF) and Vascular Endothelial 

Growth Factor (VEGF). Generally, VEGF 

leads to higher endothelial cell proliferation 

in culture. But, because of its multiple 

effects on cell metabolism, it may 

also interfere with certain experimental 

setups. In these cases, we recommend 

the use of Endothelial Cell Growth Medium, 

which contains no VEGF. 

The media are optimized for primary 

human cells but can also be used for 

bovine, porcine, murine, caprine, rabbit, 

elephant, dog, and rat endothelial cells 

from large vessels as well as for various 

endothelial cell lines. 





Endothelial Cell Growth Medium Endothelial Cell Growth Medium 2 

Fetal Calf Serum 0.02 ml / ml 0.02 ml / ml 

Endothelial Cell Growth Supplement 0.004 ml / ml — 



0.1 ng / ml 5 ng / ml 


1 ng / ml 10 ng / ml 


Insulin-like Growth Factor (Long R3 IGF) — 20 ng / ml 

Vascular Endothelial Growth Factor 165 

— 0.5 ng / ml 


Ascorbic Acid — 1 µg / ml 

Heparin 90 μg / ml 22.5 µg / ml 

Hydrocortisone 1 µg / ml 0.2 µg / ml 






500 ml C-22010 

Endothelial Cell Growth Medium Kit 500 ml C-22110 

Endothelial Cell Basal Medium 500 ml C-22210 

Endothelial Cell Basal Medium, 



SupplementMix 





500 ml C-22215 

for 500 ml C-39215 

for 500 ml C-39210 

500 ml C-22011 

Endothelial Cell Growth Medium 2 Kit 500 ml C-22111 

Endothelial Cell Basal Medium 2 500 ml C-22211 

Endothelial Cell Basal Medium 2, 



SupplementMix 



500 ml C-22216 

for 500 ml C-39216 

for 500 ml C-39211

70 


Low Serum 

4.1.4 Endothelial Cell Media MV 

Endothelial Cell Growth 

Medium MV 

Endothelial Cell Growth 

Medium MV2 




Human Coronary Artery Endothelial 



(see page 13) 







PromoCell Endothelial Cell Growth Medium 

MV and Endothelial Cell Growth 

Medium MV2 were developed for the 

in vitro cultivation of endothelial cells 

from microvascular vessels, the coronary 

artery, and the aorta. 


lacks the Endothelial Cell Growth Supplement 

(ECGS, bovine hypothalamic 

extract) but contains the basic Fibroblast 

Growth Factor, the Insulin-like Growth 

Factor (Long R3 IGF-1) and the Vascular Endothelial 

Growth Factor (VEGF). Generally, 

VEGF leads to higher endothelial cell proliferation 

in culture. But, because of its multiple 

effects on the cell metabolism, it may also 

interfere with certain experimental setups. 

In these cases, we recommend the use of 

Endothelial Cell Growth Medium MV. 

The media are optimized for primary 


bovine, porcine, murine, and rat endothelial 

cells from microvascular vessels as 

well as for various endothelial cell lines. 




(after addition to the medium) Endothelial Cell Growth Medium MV Endothelial Cell Growth Medium MV2 

Fetal Calf Serum 0.05 ml / ml 0.05 ml / ml 


Endothelial Cell Growth Supplement 0.004 ml / ml — 


10 ng / ml 5 ng / ml 



— 10 ng / ml 


Insulin-like Growth Factor (Long R3 IGF-1) — 20 ng / ml 

Vascular Endothelial Growth Factor 165 

— 0.5 ng / ml 


Ascorbic Acid — 1 µg / ml 

Heparin 90 μg / ml — 

Hydrocortisone 1 µg / ml 0.2 µg / ml 





500 ml C-22020 

Endothelial Cell Growth Medium MV Kit 500 ml C-22120 

Endothelial Cell Basal Medium MV 500 ml C-22220 

Endothelial Cell Basal Medium MV, 



SupplementMix 





500 ml C-22225 

for 500 ml C-39225 

for 500 ml C-39220 

500 ml C-22022 

Endothelial Cell Growth Medium MV2 Kit 500 ml C-22121 

Endothelial Cell Basal Medium MV2 500 ml C-22221 

Endothelial Cell Basal Medium MV2, 



SupplementMix 



500 ml C-22226 

for 500 ml C-39226 

for 500 ml C-39221


Serum-Free 

4.1.5 Epithelial Cell Media 

Airway Epithelial Cell Growth 

Medium 


Human Nasal Epithelial Cells 

(see page 21) 


(see page 22) 


(see page 21) 

The PromoCell Airway Epithelial Cell 

Growth Medium is a serum-free medium 

optimized for the cultivation of epithelial 

cells from large air passages. 



murine, porcine, equine, and rat airway 

epithelial cells. 



Bovine Pituitary Extract 

0.004 ml / ml 



10 ng / ml 


5 μg / ml 

Hydrocortisone 

0.5 μg / ml 

Epinephrine 

0.5 μg / ml 

Triiodo-L-thyronine 

Transferrin, holo (human) 

Retinoic Acid 

6.7 ng / ml 

10 μg / ml 

0.1 ng / ml 






500 ml C-21060 

Airway Epithelial Cell Growth Medium Kit 500 ml C-21160 

Airway Epithelial Cell Basal Medium 500 ml C-21260 

Airway Epithelial Cell Basal Medium, 



SupplementMix 



500 ml C-21265 

for 500 ml C-39165 

for 500 ml C-39160

72 


Serum-Free 

Small Airway Epithelial Cell 

Growth Medium 



(see page 22) 

PromoCell Small Airway Epithelial Cell 


developed for the cultivation of epithelial 

cells from the distal respiratory system. 

The optimal adjustment of growth factors 

in this medium ensures excellent in vitro 

growth. 

The medium is optimized for primary human 

cells but can also be used for porcine 

small airway epithelial cells. 








Epinephrine 



Retinoic Acid 

Bovine Serum Albumin-Fatty Acid Free 

(BSA-FAF) 

0.004 ml / ml 

10 ng / ml 

5 μg / ml 

0.5 μg / ml 

0.5 μg / ml 

6.7 ng / ml 

10 μg / ml 

0.1 ng / ml 

2.5 mg / ml 




Small Airway Epithelial Cell Growth 

500 ml C-21070 



500 ml C-21170 

Medium Kit 

Small Airway Epithelial Cell Basal Medium 500 ml C-21270 

Small Airway Epithelial Cell Basal Medium, 

500 ml C-21275 



for 500 ml C-39175 

Medium SupplementMix 


Medium SupplementPack 

for 500 ml C-39170


Serum-Free 

Mammary Epithelial Cell Growth 

Medium 


The PromoCell Mammary Epithelial Cell 


developed for the cultivation of epithelial 

cells from the mammary gland. 


cells but can also be used for rat and 

murine mammary epithelial cells as well 

as mamma carcinoma cell lines. 


(see page 23) 








0.004 ml / ml 

10 ng / ml 

5 μg / ml 

0.5 μg / ml 



Mammary Epithelial Cell Growth Medium 


500 ml C-21010 


500 ml C-21110 

Kit 

Mammary Epithelial Cell Basal Medium 500 ml C-21210 

Mammary Epithelial Cell Basal Medium, 

500 ml C-21215 



for 500 ml C-39115 

SupplementMix 



for 500 ml C-39110 

4 Media and Sera

74 


Serum-Free/Low Serum 


Medium 


Medium 2 



(see page 23) 


(see page 24) 

The PromoCell Renal Epithelial Cell 


for the cultivation of epithelial cells from 

the kidneys. 

The PromoCell Renal Epithelial Cell 

Growth Medium 2 is an optimized lowserum 

(5% V/V) medium with epinephrine. 

The combination of low serum and 

selected growth factors ensures successful 

growth in vitro and makes this medium 

the first choice for renal epithelial cells. 

The media are optimized for primary human 

cells but can also be used for porcine 

renal epithelial cells. 




(after addition to the medium) Renal Epithelial Cell Growth Medium Renal Epithelial Cell Growth Medium 2 

Fetal Calf Serum — 0.05 ml / ml 



10 ng / ml 10 ng / ml 

Insulin (recombinant human) 5 μg / ml 5 μg / ml 

Epinephrine — 0.5 µg / ml 

Hydrocortisone 36 ng / ml 36 ng / ml 


Transferrin, holo (human) 5 μg / ml 5 μg / ml 

Triiodo-L-thyronine 4 pg / ml 4 pg / ml 



Renal Epithelial Cell Growth Medium 


500 ml C-26001 

Renal Epithelial Cell Growth Medium Kit 500 ml C-26011 

Renal Epithelial Cell Basal Medium 500 ml C-26021 

Renal Epithelial Cell Basal Medium, 



SupplementMix 



Renal Epithelial Cell Growth Medium 2 


500 ml C-26022 

for 500 ml C-39604 

for 500 ml C-39600 

500 ml C-26030 

Renal Epithelial Cell Growth Medium 2 Kit 500 ml C-26130 

Renal Epithelial Cell Basal Medium 2 500 ml C-26230 

Renal Epithelial Cell Basal Medium 2, 



SupplementMix 



500 ml C-26235 

for 500 ml C-39606 

for 500 ml C-39605


Low Serum 


Medium 



(see page 24) 

The PromoCell Placental Epithelial Cell 

Growth Medium is a low-serum (5% 

V/V) medium optimized for the cultivation 

of epithelial cells from the amniotic 

membrane of the placenta. The combination 

of low serum und selected growth 

factors ensures successful growth in vitro. 









Epinephrine 




0.05 ml / ml 

10 ng / ml 

5 μg / ml 

0.5 µg / ml 

36 ng / ml 

5 μg / ml 

4 pg / ml 



Placental Epithelial Cell Growth Medium 

500 ml C-26040 



500 ml C-26140 

Medium Kit 

Placental Epithelial Cell Basal Medium 500 ml C-26240 

Placental Epithelial Cell Basal Medium, 

500 ml C-26245 



for 500 ml C-39612 

SupplementMix 



for 500 ml C-39610 

4 Media and Sera

76 


Serum-Free 

4.1.6 Fibroblast Media 



Normal Human Dermal Fibroblasts, 

juvenile foreskin (see page 25) 

The PromoCell Fibroblast Growth Medium 

is a serum-free medium optimized 

for the cultivation of neonatal and juvenile 

fibroblasts. 


cells but can also be used for rat, 

porcine, and bovine fibroblasts as well as 

fibroblast cell lines. 






1 ng / ml 

5 μg / ml 



Fibroblast Growth Medium (Ready-to-use) 500 ml C-23010 

Fibroblast Growth Medium Kit 500 ml C-23110 

Fibroblast Basal Medium 500 ml C-23210 

Fibroblast Basal Medium, phenol red-free 500 ml C-23215 



SupplementMix 



for 500 ml C-39315 

for 500 ml C-39310 

Low Serum 



Normal Human Dermal Fibroblasts, 

adult donor (see page 25) 


(see page 26) 


(see page 26) 

Human Uterine Fibroblasts 

(see page 27) 

Human Villious Mesenchymal 

Fibroblasts (see page 27) 

PromoCell Fibroblast Growth Medium 2 

is a low-serum (2% V/V) medium developed 

for adult human fibroblasts. 



porcine, and bovine fibroblasts as well as 

fibroblast cell lines. 









0.02 ml / ml 

1 ng / ml 

5 μg / ml






500 ml C-23020 

Fibroblast Growth Medium 2 Kit 500 ml C-23120 

Fibroblast Basal Medium 2 500 ml C-23220 

Fibroblast Basal Medium 2, 



SupplementMix 



500 ml C-23225 

for 500 ml C-39325 

for 500 ml C-39320 




(see page 26) 

PromoCell Fibroblast Growth Medium 3 

has been developed for the in vitro cultivation 

of adult Human Cardiac Fibroblasts 

(HCF). The medium is optimized 

for primary human cells but can also be 

used for porcine cardiac fibroblasts. 

Related products: 

Fetal calf serum (see page 92) 







0.1 ml / ml 

1 ng / ml 

5 μg / ml 






500 ml new C-23025 

Fibroblast Growth Medium 3 Kit 500 ml new C-23130 

Fibroblast Basal Medium 3 500 ml new C-23230 

Fibroblast Basal Medium 3, 



SupplementMix 



500 ml new C-23235 

for 500 ml new C-39345 

for 500 ml new C-39350

78 


Low Serum 

4.1.7 Follicle Dermal Papilla Cell Medium 


Medium 



(see page 28) 

The PromoCell Follicle Dermal Papilla 

Cell Growth Medium is a low-serum 

(4% V/V) medium. The combination of 

low serum and growth factors leads to 

excellent proliferation of human follicle 

dermal papilla cells in vitro. 










0.04 ml / ml 

0.004 ml / ml 

1 ng / ml 

5 μg / ml 





500 ml C-26501 



500 ml C-26502 

Medium Kit 

Follicle Dermal Papilla Cell Basal Medium 500 ml C-26500 

Follicle Dermal Papilla Cell Basal Medium, 

500 ml C-26505 



for 500 ml C-39625 




for 500 ml C-39620


Serum-Free 

4.1.8 Hepatocyte Media 




Human Hepatocytes (see page 29) 

The PromoCell Hepatocyte Growth Medium 

is a serum-free medium developed 

for the cultivation of human hepato- 

cytes. It is supplemented with well chosen 

growth factors and hormones and 

suited for long-term cultivation. 

In situations where the media compounds 

of the Hepatocyte Growth Medium interfere 

with certain experimental setups, we 

recommend using the PromoCell Hepatocyte 

Maintenance Medium. This medium 

is developed for short-term cultivation 

and contains fewer supplements. 

Note: Under standard in vitro cell culture 

conditions, mature human hepatocytes 

usually do not survive for longer than 

10-14 days and do not proliferate. 


hepatocytes but can also be used 

for porcine hepatocytes. 


(after addition to the medium) Hepatocyte Growth Medium Hepatocyte Maintenance Medium 



10 ng / ml — 

Insulin (recombinant human) 5 μg / ml 6 ng / ml 

Hydrocortisone 0.5 μg / ml — 

Transferrin, holo (human) 10 μg / ml — 

Ascorbic Acid 250 µg / ml — 

Bovine Serum Albumin-Fatty Acid Free 

3.75 mg / ml — 

(BSA-FAF) 

Dexamethasone — 400 ng / ml 






500 ml C-25010 

Hepatocyte Growth Medium Kit 500 ml C-25110 

Hepatocyte Basal Medium 500 ml C-25210 

Hepatocyte Basal Medium, 



SupplementMix 





500 ml C-25215 

for 500 ml C-39642 

for 500 ml C-39640 

500 ml C-25020 

Hepatocyte Maintenance Medium Kit 500 ml C-25120 


SupplementMix 



for 500 ml C-39652 

for 500 ml C-39650

80 


Serum-Free 

4.1.9 Keratinocyte Medium 




Keratinocytes, juvenile foreskin 

(see page 30) 


Keratinocytes, adult donor 

(see page 30) 

PromoCell Keratinocyte Growth Medium 

2 is a serum-free medium developed 

for the cultivation of epidermal keratinocytes 

without feeder cells. 


cells but can also be used for horse 

and porcine keratinocytes, as well as 

mouse and rat keratinocytes after adapting 

the CaCl 2 

concentration. In addition, 

it is suited for rat corneal keratinocytes. 


CaCl 2 

-Solution (see page 104) 









Epinephrine 


CaCl 2 

0.004 ml / ml 

0.125 ng / ml 

5 μg / ml 

0.33 μg / ml 

0.39 μg / ml 

10 μg / ml 

0.06 mM 





500 ml C-20011 

Keratinocyte Growth Medium 2 Kit 500 ml C-20111 

Keratinocyte Basal Medium 2 500 ml C-20211 

Keratinocyte Basal Medium 2, 



SupplementMix 



500 ml C-20216 

for 500 ml C-39016 

for 500 ml C-39011


Serum-Free 

4.1.10 Melanocyte Media 

Melanocyte Growth Medium 


Normal Human Epidermal Melanocytes, 

juvenile foreskin (see page 31) 

The PromoCell Melanocyte Growth 

Medium is a serum-free, PMA (Phorbol 

Myristate Acetate) containing medium 

optimized for the cultivation of human 

melanocytes. Since PMA is a tumor promoting 

mitogen, it may interfere with 

some experimental approaches. In these 

cases, we recommend using Melanocyte 

Growth Medium M2. 


cells but can also be used for horse, 

porcine, murine, and rat melanocytes. 








Phorbol Myristate Acetate 

0.004 ml / ml 

1 ng / ml 

5 μg / ml 

0.5 μg / ml 

10 ng / ml 



Melanocyte Growth Medium (Ready-to-use) 500 ml C-24010 

Melanocyte Growth Medium Kit 500 ml C-24110 

Melanocyte Basal Medium 500 ml C-24210 

Melanocyte Basal Medium, phenol red-free 500 ml C-24215 

Melanocyte Growth Medium SupplementMix for 500 ml C-39415 

Melanocyte Growth Medium SupplementPack for 500 ml C-39410 


Serum-Free 




Melanocytes, juvenile foreskin, 

cultured in M2 Medium (see page 31) 


Melanocytes, adult donor, cultured 

in M2 Medium (see page 31) 

The unique PromoCell Melanocyte 

Growth Medium 2 is a serum-free and 

PMA (Phorbol Myristate Acetate) free 

medium optimized for the cultivation of 

human melanocytes. 

It is optimized for primary human cells 

but can also be used for equine, porcine, 

murine, and rat melanocytes. It is also 

suitable for human pigment epithelial 

cells, neuronal cells and melanoma cells. 

List of supplements and their concentrations: confidential 






(Ready-to-use), phenol red-free 

500 ml C-24300 

500 ml C-24305 

Melanocyte Basal Medium M2 500 ml C-24400 

Melanocyte Basal Medium M2, 



SupplementMix 

500 ml C-24405 

for 500 ml C-39420

82 


4.1.11 Osteoblast Media 



Human Osteoblasts (see page 32) 

PromoCell Osteoblast Growth Medium 

was developed to establish and maintain 

human osteoblast cultures. The 

fetal calf serum in the medium is strictly 

pretested for optimal performance. 


cells but can also be used for bovine, 

murine, and rat osteoblasts as well 

as osteoblast cell lines. 

Final supplement concentration 



0.1 ml / ml 





500 ml C-27001 

Osteoblast Basal Medium 500 ml C-27010 

Osteoblast Basal Medium, 



SupplementMix 

500 ml C-27015 

for 500 ml C-39615 


Osteoblast Mineralization Medium 

PromoCell Osteoblast Mineralization 

served after 21 days, following an easy 

Medium was developed for the effi- 

one-step protocol (see page 32, Fig. 3). 


cient induction of strong mineralization 

in human osteoblast cultures. Extensive 

Human Osteoblasts (see page 32) 

extracellular calcium deposits can be ob- 




Osteoblast Mineralization Medium 100 ml C-27020


Low Serum 

4.1.12 Preadipocyte/Adipocyte Media 


Preadipocyte Differentiation 

Medium 

Adipocyte Nutrition Medium 


The PromoCell Preadipocyte Growth 

Medium is a low-serum (5% V/V) medium 

optimized for the expansion of human 

preadipocytes. 

The Preadipocyte Differentiation Medium 

is a serum-free medium, which 

induces the differentiation of preadipocytes 

into mature adipocytes. 


cells but can also be used for murine 

preadipocytes/adipocytes. 




(see page 33) 

The mature adipocytes can be cultivated 

using the Adipocyte Nutrition Medium. 



Preadipocyte 

Growth Medium 

Preadipocyte 

Differentiation Medium 

Adipocyte 

Nutrition Medium 

Fetal Calf Serum 0.05 ml / ml — 0.03 ml / ml 

Endothelial Cell Growth Supplement 0.004 ml / ml — — 



10 ng / ml — — 

Hydrocortisone 1 μg / ml — — 

d-Biotin — 8 μg / ml 8 μg / ml 

Insulin (recombinant human) — 0.5 μg / ml 0.5 μg / ml 

Dexamethasone — 400 ng / ml 400 ng / ml 

IBMX — 44 μg / ml — 

L-Thyroxine — 9 ng / ml — 

Ciglitazone — 3 μg / ml — 

Heparin 90 μg / ml — — 




Preadipocyte Growth Medium (Ready-to-use) 500 ml C-27410 

Preadipocyte Growth Medium Kit 500 ml C-27417 

Preadipocyte Basal Medium 500 ml C-27411 

Preadipocyte Basal Medium, 



SupplementMix 





500 ml C-27415 

for 500 ml C-39425 

for 500 ml C-39427 

500 ml C-27436 

Preadipocyte Differentiation Medium Kit 500 ml C-27437 


SupplementMix 



for 500 ml C-39436 

for 500 ml C-39437 

Adipocyte Nutrition Medium (Ready-to-use) 500 ml C-27438 

Adipocyte Nutrition Medium Kit 500 ml C-27439 

Adipocyte Basal Medium 500 ml C-27431 

Adipocyte Basal Medium, phenol red-free 500 ml C-27435 

Adipocyte Nutrition Medium SupplementMix for 500 ml C-39438 

Adipocyte Nutrition Medium SupplementPack for 500 ml C-39439

84 


Low Serum 

4.1.13 Skeletal Muscle Cell Media 

Skeletal Muscle Cell Growth 

Medium 


Medium 


Human Skeletal Muscle Cells 

(see page 34) 

The PromoCell Skeletal Muscle Cell 

Growth Medium is a low-serum (5% 

V/V) medium optimized for the expansion 

of human skeletal muscle cells. 

The unique Skeletal Muscle Cell Differentiation 

Medium is a serum-free 

medium that induces the fusion of 

myoblasts to myotubes with typical 

multinucleated syncytia. 



murine, and rabbit skeletal muscle cells. 







Skeletal Muscle Cell 

Growth Medium 

Skeletal Muscle Cell 


Fetal Calf Serum 0.05 ml / ml — 

Fetuin (bovine) 50 μg / ml — 



10 ng / ml — 



1 ng / ml — 


Insulin (recombinant human) 10 μg / ml 10 μg / ml 

Dexamethasone 0.4 μg / ml — 





500 ml C-23060 

Skeletal Muscle Cell Growth Medium Kit 500 ml C-23160 

Skeletal Muscle Cell Basal Medium 500 ml C-23260 

Skeletal Muscle Cell Basal Medium, 



SupplementMix 







500 ml C-23265 

for 500 ml C-39365 

for 500 ml C-39360 

500 ml C-23061 

for 500 ml C-39366


Low Serum 

4.1.14 Smooth Muscle Cell Medium 


Medium 2 









(see page 35) 




(see page 37) 

Human Bladder Smooth Muscle Cells 

(see page 38) 

Human Prostate Smooth Muscle Cells 

(see page 39) 


(see page 39) 

The PromoCell Smooth Muscle Cell 

Growth Medium 2 is a low-serum (5% 

V/V) medium developed to establish and 

maintain smooth muscle cell cultures. 



bovine, porcine, murine, dog, and rat 












0.05 ml / ml 

0.5 ng / ml 

2 ng / ml 

5 μg / ml 



Smooth Muscle Cell Growth Medium 2 

500 ml C-22062 


Smooth Muscle Cell Growth Medium 2 Kit 500 ml C-22162 

Smooth Muscle Cell Basal Medium 2 500 ml C-22262 

Smooth Muscle Cell Basal Medium 2, 

500 ml C-22267 



SupplementMix 

for 500 ml C-39267 




for 500 ml C-39262 

4.1.15 Freezing Medium 

Serum-Free 



Animal Primary Cells 

Cell Lines 

The PromoCell Cryo-SFM is an animal 

component-free, chemically defined medium 

with DMSO, methylcellulose, and 

other cryoprotective components developed 

for the cryopreservation of human 

and animal primary cells. It ensures high 

cell viability, excellent growth rates, and 

good attachment of the cells after thawing. 

The medium is the first choice for all 

types of primary cells and cell lines. 



Cryo-SFM 

30 ml 

125 ml 

C-29910 

C-29912

86 


4.2. Media for Stem and Blood Cells 

The PromoCell media for stem and blood 

cells are formulated for either the optimal 

growth of specific cell types or to 

support the differentiation into the respective 

mature cells. 

Each lot is tested for its ability to support 

growth or differentiation, respectively. In 

addition, biochemical and sterility tests 

are performed*. 

The Media (Ready-to-use) consist of a 

100 ml, 250 ml, or 500 ml bottle of Basal 

Medium and a vial of SupplementMix. 

Adding the SupplementMix to the Basal 

Medium results in the complete medium. 

Some Media are provided with additional 

Cytokine Mixes to achieve optimal 

performance. 


9 months (at 4 to 8°C), and that of the 

SupplementMix is 12 months (at -20°C) 

from the date of manufacture. After 

adding the SupplementMix, the shelf life 

of the complete medium is 6 weeks (at 

4 to 8°C). 

Note: The media for stem and blood cells 

do not contain antibiotics. 

If you require special media modifications we offer a Custom Media Service starting at 10 bottles per order. 

Please ask for details. 

4.2.1 Mesenchymal Stem Cell Media 



Growth Medium 


Growth Medium DXF 


Differentiation Media 

The PromoCell Mesenchymal Stem Cell 

Growth Medium supports the expansion 

of multipotent human Mesenchymal 

Stem Cells (MSC) without inducing early 

senescence and differentiation as observed 

in standard culture media. 

The Mesenchymal Stem Cell Growth 

Medium DXF allows for the robust 

isolation from human bone marrow is 

supported as well. 

In addition, PromoCell offers five 

media to efficiently induce differentiation 

of MSC into adipogenic, chondrogenic, 

osteogenic or neurogenic lineages, respectively 

(see also Fig. 3, page 48). 

proliferation of multipotent MSC in a 


chemically defined and xeno-free culture 

environment. It is devoid of all animal- 

Human Mesenchymal Stem Cells from 

derived components and substances 

Bone Marrow, Umbilical Cord Matrix, 

of human origin with Human Serum 

and Adipose Tissue (see page 47) 

Albumin as the only exception. Primary 




Mesenchymal Stem Cell Growth Medium 


Mesenchymal Stem Cell Growth Medium 

DXF (Ready-to-use) 


Differentiation Medium (Ready-to-use) 






Mesenchymal Stem Cell Osteogenic 


Mesenchymal Stem Cell Neurogenic 


500 ml C-28010 

500 ml 

new 

C-28019 

100 ml C-28011 

100 ml C-28012 

100 ml C-28014 

100 ml C-28013 

100 ml C-28015 

*Certificates of analysis are available on request.


4.2.2 Pericyte Medium 

Pericyte Growth Medium 


Human Pericytes (see page 49) 

PromoCell Pericyte Growth Medium has 

been developed and optimized for the in 

vitro cultivation of human pericytes from 

all tissues. 

Whereas in standard culture media pericytes 

tend to grow slowly and induce 

early senescence, PromoCell Pericyte 

Growth Medium optimally supports cell 

proliferation and maintenance of multipotency 

in these cells. 




Pericyte Growth Medium (Ready-to-use) 500 ml C-28040 

4.2.3 Hematopoietic Progenitor Cell Media 


sion of primitive HPCs in a fully defined, 

defined formula contains recombinant hu- 



xeno-free culture environment. Due to 

the utilization of exclusively synthetic, 

recombinant or plant-sourced materials, 

man TPO, SCF, flt-3 ligand and IL-3. The 

optimized formulation allows vigorous 

expansion of hematopoietic progenitor 



Cord Blood (see page 54) 

this medium is chemically defined and 

free of all animal-derived components 

and substances of human origin with 

Human Serum Albumin as the only 

exception. 

cells, while maintaining their primitive 

phenotype. Differentiation is largely 

inhibited. 

The PromoCell Hematopoietic Pro- 



genitor Medium has been developed 

(see page 50) 

Note: The medium must be supple- 

for the expansion of hematopoietic 


mented with additional cytokines, e.g. 

progenitors by means of differentiation 

(see page 51) 

Cytokine Mix E (see below). Individual 

into more committed lineages of blood 

Human Hematopoietic Progenitor 

cytokines can be found in the PromoKine 

cells. Hence, this progenitor medium 


catalog. 

is suitable for CFU-based assays, e.g. 

long-term culture initiating cell (LTC-IC) 

The PromoCell Hematopoietic Progenitor 

Cytokine Mix E is a ready-to-use mixture 

assays. 

Cell (HPC) Expansion Medium DXF has 

of growth factors for the HPC Expansion 

been designed for the maximum expan- 

Medium DXF. The animal component-free, 








500 ml C-28021 

Cytokine Mix E 

for HPC Expansion Medium DXF 

1 ml (sufficient for 100 ml Medium) 

5 ml (sufficient for 500 ml Medium) 

C-39890 

C-39891 



100 ml C-28020

88 


4.2.4 Endothelial Progenitor Medium 




(see page 54) 


(see page 50) 

Human Endothelial Progenitor Cells 

The PromoCell Endothelial Progenitor 

Medium has been developed for the 

expansion of endothelial progenitors by 

means of differentiation into more committed 

lineages of endothelial cells. 

Hence, this progenitor medium is suitable 

for the differentiation of primitive 

progenitors into mature endothelial cells 

as well as for CFU-based assays, e.g. the 

“Hill-Assay” for endothelial progenitor 

cells (Hill et al. 2003, N Engl J Med 

348: 593-600), which is carried out on 

fibronectin-coated plates. 






100 ml C-28025 

4.2.5 Dendritic Cell Media 


DC Generation Medium 

DC Generation Medium DXF 

Monocyte Attachment Medium 

PromoCell DC Generation Medium allows 

for the generation of DC from 

cryopreserved as well as freshly isolated 

cells. In the latter case, the Monocyte Attachment 

Medium is also needed. 

The Monocyte Attachment Medium 

allows for the efficient adherence selection 

of Monocytes from freshly isolated 

Mononuclear Cells (MNC) while 

maintaining optimal cell health. Thus, 

the time-consuming and costly immuno- 


The DC Generation Medium DXF is a 

magnetic purification of monocytes prior 

chemically defined and xeno-free for- 

to dendritic cell generation is not neces- 

Human Monocytes, freshly isolated 

mulation for use with freshly isolated 

sary and can be skipped. 

and cryopreserved (see page 52) 

cells. It is free of all animal derived com- 

Human Mononuclear Cells, freshly 

ponents and substances of human origin 

Note: See our Instruction Manual and 

isolated 

with Human Serum Albumin as the only 

Application Note for details. Please visit 

exception. 

www.promocell.com/application-notes 

The PromoCell Dendritic Cell (DC) Generation 

Media have been developed for the 

The DC Base Media do not include cy- 

easy and efficient generation of immature 

tokines and must therefore be supple- 

as well as fully mature myeloid Dendritic 

mented according to the user’s individual 

Cells from peripheral blood monocytes. 

needs. 


www.promocell.com/knowledge-base





DC Generation Medium (Ready-to-use) 250 ml 

new 

C-28050 

DC Generation Medium DXF 


250 ml new C-28052 

DC Base Medium 250 ml new C-28053 

DC Base Medium DXF 250 ml new C-28054 

Monocyte Attachment Medium 


250 ml new C-28051 

4.2.6 Mononuclear Cell Medium 

Mononuclear Cell Medium 

Human Monocytes (see page 52) 


The PromoCell Mononuclear Cell Medium 

is a very rich short-term maintenance 

medium which provides utmost 


from Cord Blood (see page 54) cell viability. 


from Peripheral Blood (see page 54) It is suitable to maintain MNCs for up to 


48 hours before proceeding with your 

experiments. 


Lymphocyte Separation Medium 

1077 (see below) 




Mononuclear Cell Medium (Ready-to-use) 500 ml C-28030 

4.2.7 Blood Cell Separation Medium 

Lymphocyte Separation Medium 

1077 


Peripheral blood 

Umbilical cord blood 

Buffy coats 

Apheresis products 

Bone marrow 

The PromoCell Lymphocyte Separation 

Medium 1077 is a sterile, ready-to-use 

solution optimized for the separation of 

lymphocytes from whole blood or buffy 

coats without any loss of viability. It is 

based on Ficoll 400 and sodium diatrizoate 

and provides optimal physiological 

parameters. It has a density of 1,077 g/ml 

at +20°C. 



Lymphocyte Separation Medium 1077 500 ml C-44010

90 


4.3 Classical Media 

• DMEM Media 



DMEM, w/o L-Glutamine 500 ml C-71110 

DMEM, with L-Glutamine 500 ml C-71111 

DMEM, with 25 mM HEPES, 

500 ml C-71115 

w/o L-Glutamine 

DMEM, with 25 mM HEPES, 

500 ml C-71116 

with L-Glutamine 

DMEM, with stable Glutamine 500 ml C-71120 

DMEM, High Glucose (4.5 g/l), 

500 ml C-71210 



500 ml C-71212 



500 ml C-71220 

with stable Glutamine 


500 ml C-71221 

with stable Glutamine, with Na-Pyruvate 


500 ml C-71222 

with L-Glutamine, with Na-Pyruvate 


w/o L-Glutamine, with Na-Pyruvate 

500 ml C-71223 


• DMEM/Ham´s F-12 Media 



DMEM/Ham´s F-12, w/o L-Glutamine 500 ml C-71310 

DMEM/Ham´s F-12, with L-Glutamine 500 ml C-71311 

DMEM/Ham´s F-12, with stable Glutamine 500 ml C-71320 

• Ham´s Media 



Ham´s F-10, w/o L-Glutamine 500 ml C-72010 

Ham´s F-10, with L-Glutamine 500 ml C-72020 

Ham´s F-12, w/o L-Glutamine 500 ml C-72110 

Ham´s F-12, with L-Glutamine 500 ml C-72120 

• Leibovitz L-15 Medium 



Leibovitz L-15, w/o L-Glutamine 500 ml C-73110


• McCoy´s 5A Media 



McCoy’s 5A, w/o L-Glutamine 500 ml C-73210 

McCoy’s 5A, with L-Glutamine 500 ml C-73211 

McCoy´s 5A, with stable Glutamine 500 ml C-73220 

McCoy´s 5A, with stable Glutamine, 

500 ml C-73227 


• MEM Alpha and MEM D-Valine Media 



MEM Alpha, w/o Nucleosides, 

500 ml C-75012 


MEM Alpha, w/o Nucleosides, 

500 ml C-75013 


MEM Alpha, with Nucleosides, 

500 ml C-75014 


MEM D-Valine, w/o L-Glutamine 500 ml C-75100 

• MEM Earle Media 




MEM Earle, w/o L-Glutamine 500 ml C-75210 

MEM Earle, with L-Glutamine 500 ml C-75211 

MEM Earle, with stable Glutamine 500 ml C-75220 

• RPMI 1640 Media 



RPMI 1640, w/o L-Glutamine 500 ml C-76010 

RPMI 1640, with L-Glutamine 500 ml C-76011 

RPMI 1640, with 25 mM HEPES, 

500 ml C-76015 


RPMI 1640, with 25 mM HEPES, 

500 ml C-76016 


RPMI 1640, w/o L-Glutamine, 

500 ml C-76017 


RPMI 1640, with stable Glutamine 500 ml C-76020

92 


4.4 Insect Cell Culture Media 

The medium requirements of insect cells 

are significantly different from vertebrate 

cell culture. For instance, the optimal pH 

range for most insect cells is more acidic 

(pH 6.2 to 6.5). Also, the amino acid 

concentration needs to be higher than in 

vertebrate cell media. 

Mitsuhashi/Maramorosh Insect 

Medium 


Mitsuhashi and Maramorosh Insect 

Medium is well-suited for Mosquito cell 

culture and must be supplemented with 

FCS prior to use. 

Mosquito Cells 



Mitsuhashi/Maramorosh Insect Medium 500 ml C-77110 


Schneider’s Drosophila Medium 


Dipteran Cells 

Schneider’s Drosophila Medium was 

originally developed for the culture of 

Drosophila cells but can also be used for 

the culture of other dipteran cell lines. 



Schneider’s Drosophila Medium, 


500 ml C-77210 

4.5 Sera 



Primary Human Cells (all types) 

The PromoCell Fetal Calf Sera are high 

quality, pretested fetal calf serum batches. 

They are extensively tested for their 

ability to support in vitro expansion of 

human primary cells. 

In addition, histochemical staining is performed 

to ensure that the cultured primary 

cells maintain their cell type specific 

marker expression. 



Fetal Calf Serum (EU Quality) 

primary human cell culture tested 

100 ml 

500 ml 

C-37355 

C-37350




Mesenchymal Stem Cells (all types) 

The PromoCell MSC-Qualified Fetal Calf 

Sera are high quality, pre-selected fetal 

calf serum batches. They are extensively 

tested for their ability to support in vitro 

expansion of human Mesenchymal Stem 

Cells. In addition, histochemical staining 

is performed to ensure that the cultured 

stem cells maintain the ability to differentiate 

into diverse lineages. 

Fig. 1: Expansion and differentiation of 

Mesenchymal Stem Cells (MSC) using 

media with the PromoCell MSC-Qualified 

Fetal Calf Serum. Left: Undifferentiated 

hMSC-BM cultured for 4 passages 

(phase contrast). Right: The same cells 

after 14 days of adipogenic induction 

(Oil Red O staining of lipid droplets 

with Hematoxylin counterstain). 




100 ml 

500 ml 

C-37386 

C-37385 

ESC-Qualified Fetal Calf Serum 

The PromoCell ESC-Qualified Fetal Calf 

factor. The maintenance of pluripotency 


Sera are high quality, pre-selected fetal 

is assayed by immunostaining for basal 


calf serum batches. They are exten- 

pluripotency markers. In addition, this 

sively tested for their ability to support 

serum is also recommended for the cul- 

Pluripotent Embryonic Stem Cells 

undifferentiated, feeder-free culture of 

ture of hematopoietic and endothelial 

Hematopoietic Progenitor Cells 

pluripotent murine embryonic stem cells 

progenitor cells. 

Endothelial Progenitor Cells 

in the presence of leukemia inhibitory 

Fig. 2: Growth morphology of murine 

embryonic stem cells (mESC). Left: 

mESC in standard Fetal Calf Serum. 

Note the large, flat, differentiated 

cells. Right: mESC after 5 passages in 

PromoCell ESC-Qualified Fetal Calf 

Serum. The cells are small and have a 

compact colony morphology typical for 

mESC. 



ESC-Qualified Fetal Calf Serum 

100 ml 

500 ml 

C-37388 

C-37387


5 

PromoCell Cell Pellets are an easily accessible source of RNA, 

DNA, or proteins from a wide range of human primary cell 

types without laborious cell cultivation work. This allows you 

to focus on your molecular biology experiments and maximize 

your valuable research time.

96 


5 


5.1 Cell Pellets new ...................... 97

5 Cell Pellets 97 

5.1 Cell Pellets 

Applications 

Gene expression analysis 

Cell characterization studies 

Northern blots 

Southern blots 

Western blots/2D gel electrophoresis 

Features 

Available from all PromoCell 

cell types 

1 million cells per pellet 

Stored in RNAlater ® 

Indefinitely stable at -20°C 

Compatible with established nucleic 

acid purification protocols 

PromoCell Cell Pellets are stored in 

RNAlater ® - an aqueous protection solution 

that rapidly permeates the cells and stabilizes 

cellular DNA, RNA, and proteins 

and protects them against degradation. 

This makes the PromoCell Cell Pellets 

very useful for a wide range of research 

applications* like gene expression analysis, 

cell characterization studies, and protein 

characterization experiments. 

Note: RNAlater ® denatures proteins. 

Therefore, proteins will be suitable for 

e.g. Western blotting but not for applications 

that require native proteins. 

Each Cell Pellet is made from 1 million 

cells and is dissolved in 200 µl RNAlater ® . 

The Cell Pellets are produced from released 

cell lots and have the same characteristics, 

i.e. expression profile or cell 

type specific antigens. On request the 

cells from the same lot are available for 

cultivation. The Cell Pellets can be stored 

indefinitely at -20°C. 


Product Product Description Catalog Number 

HAoAF Pellet Pellet from Human Aortic Adventitial Fibroblasts C-14037 

HAoEC Pellet Pellet from Human Aortic Endothelial Cells C-14023 

HAoSMC Pellet Pellet from Human Aortic Smooth Muscle Cells C-14053 

HBdMEC Pellet Pellet from Human Bladder Microvascular Endothelial Cells C-14026 

HBdSMC Pellet Pellet from Human Bladder Smooth Muscle Cells C-14057 

HBEpC Pellet Pellet from Human Bronchial Epithelial Cells C-14063 

HBMEC Pellet Pellet from Human Brain Microvascular Endothelial Cells coming soon C-14032 

HBSMC Pellet Pellet from Human Bronchial Smooth Muscle Cells C-14055 

HCAEC Pellet Pellet from Human Coronary Artery Endothelial Cells C-14022 

HCASMC Pellet Pellet from Human Coronary Artery Smooth Muscle Cells C-14052 

HCF Pellet Pellet from Human Cardiac Fibroblasts C-14036 

HCH Pellet Pellet from Human Chondrocytes C-14070 

HCM Pellet Pellet from Human Cardiac Myocytes C-14080 

HCMEC Pellet Pellet from Human Cardiac Microvascular Endothelial Cells C-14029 

HDBEC adult Pellet Pellet from Human Dermal Blood Endothelial Cells adult donor C-14019 

HDBEC Pellet 

Pellet from Human Dermal Blood Endothelial Cells 

C-14018 


HDLEC adult Pellet 

Pellet from Human Dermal Lymphatic Endothelial Cells 

C-14021 

adult donor 

HDLEC Pellet 

Pellet from Human Dermal Lymphatic Endothelial Cells 

C-14020 


HDMEC adult Pellet 

Pellet from Human Dermal Microvascular Endothelial Cells 

C-14016 

adult donor 

HDMEC Pellet 


C-14015 


HDMEC pre-screened Pellet 


C-14017 

pre-screened 

HFDPC Pellet Pellet from Human Follicle Dermal Papilla Cells C-14005 

HHpC Pellet Pellet from Human Hepatocytes C-14085 

HMEpC Pellet Pellet from Human Mammary Epithelial Cells C-14066 

hMNC-CB pooled Pellet 

Pellet from Human Mononuclear Cells from Cord Blood 

C-14097 


hMNC-CB single donor Pellet 

Pellet from Human Mononuclear Cells from Cord Blood 

C-14096 


hMNC-PB single donor Pellet 

Pellet from Human Mononuclear Cells from Peripheral Blood 

C-14098 


hMNC-PB pooled Pellet 

Pellet from Human Mononuclear Cells from Peripheral Blood 


new C-14099 

5 Cell Pellets 

* More information on RNAlater ® (FAQs, protocols) at www.invitrogen.com/site/us/en/home/brands/Product-Brand/rnalater.html

98 



Product Product Description Catalog Number 


hMoCD14 + -PB single donor Pellet 



hMo-PB single donor Pellet 

hMo-PB single donor 


Pellet from Human CD14 + Monocytes from Peripheral Blood, 

single donor 

Pellet from Human CD14 + Monocytes from Peripheral Blood, 

single donor pre-screened 

Pellet from Human Monocytes from Peripheral Blood, single 

donor 

Pellet from Human Monocytes from Peripheral Blood, single 

donor pre-screened 

C-14110 

C-14112 

C-14111 

C-14113 

hMSC-AT Pellet Pellet from Human Mesenchymal Stem Cells from Adipose Tissue C-14092 

hMSC-BM Pellet Pellet from Human Mesenchymal Stem Cells from Bone Marrow C-14090 

hMSC-UC Pellet 

Pellet from Human Mesenchymal Stem Cells 

C-14091 


HNEpC Pellet Pellet from Human Nasal Epithelial Cells C-14062 

HOB Pellet Pellet from Human Osteoblasts C-14071 

HPAEC Pellet Pellet from Human Pulmonary Artery Endothelial Cells C-14024 

HPASMC Pellet Pellet from Human Pulmonary Artery Smooth Muscle Cells C-14051 

hPC-PL Pellet Pellet from Human Pericytes from Placenta C-14095 

HPF Pellet Pellet from Human Pulmonary Fibroblasts C-14035 

HPlEpC Pellet Pellet from Human Placental Epithelial Cells C-14069 

HPMEC Pellet Pellet from Human Pulmonary Microvascular Endothelial Cells C-14027 

HPrSMC Pellet Pellet from Human Prostate Smooth Muscle Cells C-14058 

HRCEpC Pellet Pellet from Human Renal Cortical Epithelial Cells C-14068 

HREpC Pellet Pellet from Human Renal Epithelial Cells C-14067 

HSAEpC Pellet Pellet from Human Small Airway Epithelial Cells C-14065 

HSaVEC Pellet Pellet from Human Saphenous Vein Endothelial Cells C-14025 

HTEpC Pellet Pellet from Human Tracheal Epithelial Cells C-14064 

HTSMC Pellet Pellet from Human Tracheal Smooth Muscle Cells C-14056 

HUAEC Pellet Pellet from Human Umbilical Artery Endothelial Cells C-14013 

HUASMC Pellet Pellet from Human Umbilical Artery Smooth Muscle Cells C-14050 

HUF Pellet Pellet from Human Uterine Fibroblasts C-14038 

HUtMEC Pellet Pellet from Human Uterine Microvascular Endothelial Cells C-14028 

HUtSMC Pellet Pellet from Human Uterine Smooth Muscle Cells C-14059 

HUVEC Pellet Pellet from Human Umbilical Vein Endothelial Cells C-14010 

HUVEC pooled Pellet Pellet from Human Umbilical Vein Endothelial Cells pooled C-14011 

HUVEC pre-screened Pellet Pellet from Human Umbilical Vein Endothelial Cells pre-screened C-14012 

HVMF Pellet Pellet from Human Villous Mesenchymal Fibroblasts C-14034 

HWP subcutaneous Pellet Pellet from Human White Preadipocytes subcutaneous C-14072 

HWP visceral Pellet Pellet from Human White Preadipocytes visceral C-14073 

NHDF adult Pellet Pellet from Normal Human Dermal Fibroblasts adult donor C-14031 

NHDF Pellet Pellet from Normal Human Dermal Fibroblasts juvenile foreskin C-14030 

NHEK adult Pellet Pellet from Normal Human Epidermal Keratinocytes adult donor C-14002 

NHEK adult pooled Pellet 

Pellet from Normal Human Epidermal Keratinocytes adult, 

new C-14004 

pooled 

NHEK.f Pellet 

Pellet from Normal Human Epidermal Keratinocytes 

C-14001 


NHEK.f pooled Pellet 

Pellet from Normal Human Epidermal Keratinocytes 

new C-14003 


NHEM M2 adult Pellet 

Pellet from Normal Human Epidermal Melanocytes 

C-14043 

adult donor, cultured in M2 Medium 

NHEM.f M2 Pellet 


C-14042 

juvenile foreskin, cultured in M2 Medium 

NHEM.f Pellet 


C-14040 


SkMC Pellet Pellet from Human Skeletal Muscle Cells C-14060 

new 

new 

new 

new


6 

PromoCell offers a broad range of reagents and supplements 

for cell culture. This includes enzyme mixtures for tissue and 

cell dissociation, buffers for daily cell culture work, antibiotics 

and antimycotics in different concentrations, components 

to supplement various culture media, such as cytokines and 

growth factors, and reagents needed for cell assays. All of these 

are intensively tested and meet our stringent quality criteria.

100 


6 


6.1 Detachment Reagents new ............ 101 

6.2 Antibiotics and Antimycotics .......... 103 

6.3 Buffers and Salt Solutions ............ 104 

6.4 Supplements new ................... 105 

6.5 Cytokines and Growth Factors. ........ 106

6 Reagents and Supplements 101 

6.1 Detachment Reagents 

DetachKit 


The DetachKit is optimized for primary 


animal cells and cell lines. 

All human primary cell types 

The PromoCell DetachKit was developed 

for gentle and effective detachment of 

adherent primary human cells in culture. 

Each batch is tested on primary human cells. 

The kit contains three components: 

HepesBSS (30 mM Hepes) 

Trypsin / EDTA Solution 

(0.04% / 0.03%) 

Trypsin Neutralizing Solution (TNS) 



DetachKit 

30 ml 

125 ml 

250 ml 

C-41200 

C-41210 

C-41220 

DetachKit 2 

The PromoCell DetachKit 2 was developed 

for very gentle detachment of primary 

human cells in culture. It contains 

less trypsin and EDTA than the standard 

DetachKit and is suited for applications, 

which require very gentle cell handling. 

The DetachKit 2 is optimized for primary 


animal cells and cell lines. 

The kit contains three components: 

HepesBSS (30 mM Hepes) 

Trypsin / EDTA Solution 

(0.025% / 0.01%) 




DetachKit 2 

30 ml 

125 ml 

250 ml 

C-41202 

C-41212 

C-41222 

Accutase-Solution 

Accutase is an alternative cell detachment 

solution to trypsin and can also 

face proteins and epitopes stay entirely 

intact. Accutase is therefore perfectly 

6 Reagents and 

Supplements 


be used for tissue dissociation. It was 

suited for applications, which require an 

developed for very gentle and effective 

unchanged cell surface. 

Primary cells 

detachment of adherent cells. The well 

Each batch of the PromoCell Accutase- 

Cell lines 

balanced combination of proteolytic and 

Solution is tested on primary human 

Tissue dissociation 

collagenolytic activities ensures that sur- 

cells. 



Accutase-Solution, 


100 ml C-41310

102 

6 Reagents and Supplements 

Cell Dissociation Solution ACF 

Cell Dissociation Solution ACF is a proprietary 

non-enzymatic reagent for detachment 

of adherent cell types from 

tissue culture plastic or glass surfaces. 

Due to its gentle yet effective action it 

presents a good animal component-free 

alternative to trypsin and other enzymatic 

detachment solutions. 



Cell Dissociation Solution ACF 100 ml C-41320 

new 

Trypsin Solutions 

Trypsin is still the most widely employed 

enzyme used for cell detachment. 

PromoCell´s Trypsin Solutions exhibit 

standardized enzyme activity and contain 

trypsin from extra-pure lots. 



Trypsin / EDTA (0.04% / 0.03%) 

Trypsin / EDTA 2 (0.025% / 0.01%) 

30 ml 

125 ml 

250 ml 

30 ml 

125 ml 

250 ml 

C-41000 

C-41010 

C-41020 

C-41002 

C-41012 

C-41022 

Trypsin PBS 1:250 (0.25%), w/o Ca ++ /Mg ++ 100 ml C-41040 

Trypsin PBS 1:250 (2.5%), w/o Ca ++ /Mg ++ , 

100 ml C-41050 

10x conc. 

Trypsin-EDTA PBS 1:250 (0.05% / 0.02%) 100 ml C-41060 

Trypsin-EDTA PBS 1:250, (0.5% / 0.2%), 

w/o Ca ++ /Mg ++ , 10x conc. 

100 ml C-41070 


Supplements 

Trypsin Neutralizing Solution 




(0.05% Trypsin Inhibitor in 0.1% BSA) 

30 ml 

125 ml 

250 ml 

C-41100 

C-41110 

C-41120


6.2 Antibiotics and Antimycotics 

Antibiotics are used in order to prevent 

microbial contaminations in cell culture 

during initial isolation steps or to cure 

precious cultures exhibiting microbial 

contamination, e.g. with mycoplasma or 

yeast/fungi. 

However, antibiotics may not only harm 

contaminating organisms, but can also 

unfavorably alter the physiology and 

lifespan of primary human cells. Therefore 

PromoCell does not recommend 

the routine use of antibiotics in cell 

culture as a general preventive measure. 

As a further application antibiotics may 

be utilized in genetic selection procedures, 

e.g. G-418. 

• Antibiotics and Antimycotics 



Pen/Strep Solution 

(10,000 IU/ml / 10 mg/ml) 

Pen/Strep/Fungizone Solution 

(10,000 IU/ml / 10,000 μg/ml / 25 μg/ml) 

100 ml C-42010 

100 ml C-42020 

Amphotericin B Solution (0.25 mg/ml) 100 ml C-42040 

Gentamicin Solution (5 mg/ml) 100 ml C-42060 

• Selection Antibiotics 



G-418 Sulphate Solution (50 mg/ml) 20 ml C-42094 

Hygromycin B Solution (50 mg/ml) 20 ml C-42095 


Supplements

104 


6.3 Buffers and Salt Solutions 

Buffers and Salt Solutions are frequently 

used in tissue culture, cell isolation procedures, 

and molecular biology. 

For convenience, PromoCell offers 

ready-to-use Buffers and Salt Solutions. 

They are prepared from raw materials of 

the highest quality using tissue culture 

grade water. 

• Dulbecco´s Phosphate Buffered Saline 



Dulbecco’s PBS 500 ml C-40230 

Dulbecco’s PBS, w/o Ca ++ /Mg ++ 500 ml C-40232 

Dulbecco’s PBS, w/o Ca ++ /Mg ++ , 10x conc. 500 ml C-40238 

• Hanks´ Balanced Salt Solutions 



Hanks’ BSS 500 ml C-40310 

Hanks’ BSS, w/o Ca ++ /Mg ++ 500 ml C-40320 

Hanks’ BSS, phenol red-free 500 ml C-40370 

Hanks’ BSS, w/o Ca ++ /Mg ++ , 


500 ml C-40390 

• Endotoxin-free Buffers and Water 



PBS, endotoxin-free 500 ml C-40240 

PBS, w/o Ca ++ /Mg ++ , endotoxin-free 500 ml C-40242 

TC Water 500 ml C-49998 

• Other Solutions 




Supplements 

CaCl 2 

-Solution (0.5 M) 2 ml C-34006 

HEPES Buffered Saline (30 mM HEPES) 

30 ml 

125 ml 

250 ml 

C-40000 

C-40010 

C-40020 

HEPES Buffer (1 M) 100 ml C-40030 

Sodium bicarbonate Solution (7.5%) 100 ml C-42340


6.4 Supplements 

• Growth Supplements 



Endothelial Cell Growth Supplement* 

(bovine hypothalamic extract) 

Endothelial Cell Growth Supplement/ 

Heparin* (bovine hypothalamic extract) 

Bovine Pituitary Extract 15* 

(7.5 mg protein/ml) 

Bovine Pituitary Extract 26* 

(13 mg protein/ml) 

Human Insulin, 


2 ml 

10 x 2 ml 

2 ml 

10 x 2 ml 

2 ml 

10 x 2 ml 

2 ml 

10 x 2 ml 

C-30160 

C-30180 

C-30120 

C-30140 

C-30020 

C-30040 

C-30021 

C-30041 

50 mg C-52310 

Human Transferrin APO, low iron content 1 g C-52322 

Human Transferrin HOLO, iron saturated 1 g C-52332 

• Amino Acid Solutions 



L-Glutamine Solution 100 ml C-42210 

Stable Glutamine Solution 100 ml C-42215 

MEM Amino Acids Solution 100 ml C-42320 

MEM NEAA Solution 100 ml C-42322 

• Vitamin and Trace Element Solutions 



MEM Vitamin Solution 100 ml C-42328 

Trace Element Mix (1000x) 100 ml C-42331 

• Extracellular Matrix Proteins 


Collagen Type I, Rat Tail (3-4 mg/ml) 

25 mg 

100 mg 


C-43010 

C-43020 

Fibronectin Solution, bovine (1 mg/ml) 5 ml C-43050 

Fibronectin Solution, human (1 mg/ml) 5 ml new C-43060 


Supplements 

* Endothelial Cell Growth Supplement (ECGS) and Bovine Pituitary Extract (BPE) are popular growth supplements in primary cell culture. PromoCell ECGS 

and BPE are manufactured from raw material which is exclusively collected in New Zealand to ensure maximum BSE-safety.

106 


6.5 Cytokines and Growth Factors 

Under the brand PromoKine, PromoCell 

provides a wide range of human, mouse, 

and rat cytokines, chemokines, growth 

factors, soluble receptors, and CD antigens 

(> 500 items). These recombinant 

proteins have been produced in E. coli, 

yeast, insect, mammalian, or plant cells. 

They are highly purified and biologically 

active and also available in bulk sizes. For 

a complete list of available cytokines and 

growth factors please see the PromoKine 

catalog or www.promokine.info. 

Cytokines mentioned in this catalog: 

https://www.promokine.info/shop 


EGF, human, recombinant (E. coli) 500 μg C-60170 

EGF, human, recombinant (Pichia) 500 μg C-60182 

FGF-1 (FGF-a), human, recombinant 

(E. coli) 

FGF-2 (FGF-b), human, recombinant 

(E. coli) 

50 μg C-60340 

50 μg C-60240 

flt-3 Ligand, human, recombinant (E. coli) 10 μg C-67110 

G-CSF, human, recombinant (E. coli) 10 μg C-60434 

GM-CSF, human, recombinant (E. coli) 10 μg C-60420 

IL-3 CC, human, recombinant (E. coli) 30 μg C-61301 



Insulin, human, recombinant (E. coli) 25 mg C-60212 

M-CSF, human, recombinant (E. coli) 10 μg C-60442 

SCF, human, recombinant (E. coli) 10 μg C-63120 

sRANKL, human, recombinant (E. coli) 10 μg C-63200 


Supplements 

TNF-alpha, human, recombinant (E. coli) 10 μg C-63722 

TPO, human, recombinant (E. coli) 10 μg C-65112 

VEGF-165, human, recombinant (E. coli) 10 µg C-64420 

VEGF-165, human, recombinant (HEK293) 10 µg C-64423

Microbial Detection 

7 

and Elimination 

Cell culture contamination is a common problem that impacts 

many biochemical and immunological properties of cells and it 

leads to unsatisfactory and unreliable results. Therefore, regular 

testing of cell cultures for mycoplasmas/bacteria contamination 

is essential. 

PromoCell provides highly sensitive and user-friendly test kits 

for this purpose, as well as products for the effective elimination 

and prevention of mycoplasma and eubacteria contamination.

108 


7 

Microbial Detection 

and Elimination 

7.1 Mycoplasma Test Kits ............... 109 

7.2 Mycoplasma Elimination Solutions. ..... 110 

7.3 Bacteria Test Kit. ................... 112 

7.4 Disinfectants ...................... 113

7 Microbial Detection and Elimination 109 

7.1 Mycoplasma Test Kits 

Applications 

Mycoplasma detection in cell culture, 

virus stocks, and medium 

Conventional and real-time PCR 

Features 

Highly sensitive (detects 10 - 15 

mycoplasmas per sample volume) 

Fast, reproducible, and reliable results 

Applicable for all cell/tissue culturerelevant 

mycoplasma species 

Positive and internal controls included 

Convenient protocol and easy sample 

preparation 

Mycoplasmas are parasitic bacteria that 

frequently contaminate tissue cultures 

and consequently also virus stocks, vaccines, 

and other biological materials produced 

in cells. The contamination can 

originate from serum, laboratory staff, 

other contaminated cultures, or the donors 

from which the cells have been harvested, 

and it often remains unnoticed. 

Although mycoplasmas do not cause 

visible cell damage, they seriously affect 

cell growth and metabolism, transfection 

efficiency, protein and monoclonal 

antibody synthesis, cytokine secretion, 

immunological properties, signal transduction, 

virus proliferation and can even 

cause DNA and RNA damage. 

Since mycoplasmas are very small and 

lack cell walls, detection using a conventional 

light microscope is nearly 

impossible. In addition, cloudiness in 

the medium, typical for bacterial or 

yeast contaminations, does not occur 

even at high mycoplasma concentrations. 

PCR on the other hand is a highly 

sensitive, specific, and rapid method 

for the detection of mycoplasma contamination 

in culture supernatant, cell/ 

virus stocks, and medium. All cell/tissue 

culture relevant mycoplasma species can 

be detected in one assay including M. 

fermentans, M. hyorhinis, M. arginini, 

M. orale, M. salivarium, M. hominis, 

M. pulmonis, and M. pirum. In addition, 

Acholeplasma laidlawii, M. synoviae, 

and Ureaplasma species can be 

detected. 

PCR Mycoplasma Test Kit I/C 

The PCR Mycoplasma Test Kit I/C utilizes 

conventional PCR technology for 

convenient and specific detection of 

more than 25 contaminating mycoplasma 

species. The primer set is specific 

for the highly conserved 16S-rRNA coding 

region in the mycoplasma genome. 

The test can be performed in 3 hours 

and includes internal control DNA (for 

verifying a successful PCR run) as well 

as positive control DNA. All components 

required for the PCR (primers, nucleotides, 

internal control DNA) come optimally 

premixed and lyophilized in PCR 

tubes (except the Taq polymerase). For 

setting up the PCR, simply cut off the 

number of tubes required, rehydrate 

with rehydration buffer (containing the 

Hot-Start Taq polymerase), and add the 

sample to be analyzed. Samples can be 

easily prepared by boiling the cell culture 

supernatant or other sample material. 

PCR Mycoplasma Test Kit I/RT 

The PCR Mycoplasma Test Kit I/RT utilizes 

real-time PCR technology for convenient 

and reproducible detection of 

contaminating mycoplasmas. It shows 

excellent sensitivity and specificity and 

enables reliable, quantitative PCR results 

quickly (making the kit particularly 

useful for routine testing). The primer 

and probe set is specific for the highly 

conserved 16S-rRNA coding region in 

the mycoplasma genome. It contains 

FRET probes emitting fluorescent light 

at 520 nm and is compatible with most 

common Real-Time PCR cyclers (e.g. 

Roche Light Cycler ® 1 and 2, ABI Prism ® , 

Cepheid Smart Cycler ® , and Eppendorf 

Mastercycler ® ep realplex). 

The test includes internal control DNA 

(for verifying a successful PCR run) as 

well as positive control DNA. All components 

required for real time PCR 

(primers, probes, nucleotides, and internal 

control DNA) come lyophilized 

in PCR tubes, except the Hot-Start Taq 

polymerase which is dissolved in reaction 

buffer. Two kit variants (kit A for 

e.g. Light Cycler, kit B for e.g. ABI Prism) 

are provided which have been optimized 

for different Real Time PCR instruments. 

For recommended kit variant, please see 

www.promokine.info where you can 

download the manual. 

PCR 

100 µl cell culture 

supernatant 

Boil for 10 minutes 

and centrifuge briefly 

2 µl supernatant 

Analyze 5 µl of 

the PCR sample 

7 Microbial 

Detection and 

Elimination 

Fig. 1: Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient: Boil 100 µl cell culture 

supernatant or other sample material for 10 minutes; centrifuge briefly and add 2 µl supernatant to the reaction cup with the 

rehydrated PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis.

110 

7 Microbial Detection and Elimination 

PCR Mycoplasma Test Kit III 

The PCR Mycoplasma Test Kit III utilizes 

conventional PCR technology for convenient 

and highly sensitive detection of 

mycoplasma. The primer set is specific for 

the highly conserved 16S-rRNA coding 

region in the mycoplasma genome. Thus, 

the kit reliably detects all mycoplasma 

commonly found in cell culture, including 

the Acholeplasma and Spiroplasma 

genera. The kit has been positively tested 

against all mycoplasma strains listed in the 

European Pharmacopoeia, but may detect 

even more mycoplasma species (~70- 

120 species). It does not detect microorganisms 

such as Streptococcus sp., 

Clostridium sp. and Lactobacillus sp. 

which are phylogenetically closely related. 

The kit includes all the components required 

for PCR (nucleotides, primers, a 

high-performance Taq polymerase, and 

an agarose gel loading dye), all premixed 

at optimal concentrations for the preparation 

of a ready-to-use and ready-toload 

1x master mix. The test takes approximately 

3 hours to run and obtain 

clear results. The kit also contains positive 

control DNA for easy result verification as 

well as an internal control DNA to verify a 

successful PCR run. 

M 1 2 3 4 5 6 

1 2 3 4 5 6 7 M 

479 bp 

270 bp 

260 bp 

160 bp 

Fig. 2 Top: Detection of mycoplasmas 

with PCR Mycoplasma Test Kit I/C. 

M: 100 bp DNA ladder 

1: Positive control 

2-6: 10; 100; 1,000; 10,000; 100,000 

mycoplasmas/sample volume 

Internal control DNA: 479 bp band 

Positive sample/control: 270 bp band 

Bottom: Detection of mycoplasmas 

with PCR Mycoplasma Test Kit III. 

1-4: A. laidlawii, S. citri, M. synoviae, 

and M. orale (50 cfu/ml each) 

5: Cell culture supernatant, not infected 

6: Negative control 

7: Positive control 

M: DNA size standard 

Internal control DNA: 160 bp band 

Positive sample/control: 260-270 bp band 



PCR Mycoplasma Test Kit I/C 

24 tests 

48 tests 

96 tests 

PK-CA91-1024 

PK-CA91-1048 

PK-CA91-1096 

PCR Mycoplasma Test Kit I/RT; Variant A 25 tests PK-CA91-3025A 

PCR Mycoplasma Test Kit I/RT; Variant B 25 tests PK-CA91-3025B 

PCR Mycoplasma Test Kit III 100 tests PK-CA92-100 

RTU DNA Ladder I (100 bp) 0.5 ml PK-MB713-001N 

RTU DNA Ladder II (1000 bp) 0.5 ml PK-MB713-002N 

Please see Chapter 1.11 of the PromoKine Catalog for the ready-to-use DNA Ladders. 

7 Microbial 

Detection and 

Elimination 

7.2 Mycoplasma Elimination Solutions 

Mycoplasma contamination seriously affects 

cell growth and cell physiology leading 

to biased and unreliable experimental 

results. When mycoplasma contamination 

is detected, the contaminated cells 

are ideally discarded and all equipment, 

e.g. work benches, CO 2 

incubators, etc., 

are disinfected (see chapter 7.4 for disinfectant 

sprays). However, if the affected 

cells are unique or the result of 

extensive work, thorough elimination of 

the contaminating mycoplasmas is necessary. 

Standard antibiotics commonly 

used in cell culture (e.g. Pen/Strep) do 

not affect mycoplasmas. Therefore, 

PromoKine offers two highly effective 

methods to get rid of mycoplasma contamination: 

Mycoplasma-EX, a combination 

of non-antibiotic and antibiotic 

reagents, and BIOMYC 1-3, three highly 

effective antibiotics.


Mycoplasma-EX 

Applications 

Mycoplasma elimination in cell/tissue 

culture 

Mycoplasma elimination in virus 

stocks 

Features 

Highly effective against mycoplasma 

species commonly found in cell 

cultures 

Very short treatment time 

Very low cytotoxicity 

Ready to use 

Easy handling 

Mycoplasma-EX combines a non-antibiotic 

and an optimized antibiotic treatment 

for the effective elimination of 

mycoplasmas and other microorganisms 

from cell/tissue culture and virus stocks. 

It eliminates mycoplasmas by directly 

killing them and not just by inhibiting 

their growth, with minimal toxicity for 

the cells in culture. 

Mycoplasma-EX is effective after a single 

treatment, and destroys mycoplasmas 

rapidly and permanently. It is applicable 

for most cell lines (e.g. Vero, BHK21, 

GBK, ML, Hep2, 293, CRFK, H9, Molt4, 

MT-4, Jurkat), as well as virus stocks 

(e.g. SHV-1, BHV-1, HSV-1, VSV, SFV, 

FCV, MEV) and is highly effective against 

M. orale, M. hyorhinis, M. hominis, 

Acholeplasma laidlawii, M. arginini, M. 

fermentans, and other mycoplasma species 

encountered as contaminants in cell 

cultures. 

BIOMYC Antibiotic 

Solutions 

Applications 

Mycoplasma elimination in cell/ 

tissue culture 

Features 

Highly effective against mycoplasma 

species commonly found in cell 

cultures 

Low cytotoxicity 

Ready to use 

Easy handling 

Antibiotics that impact bacterial cell walls 

(e.g. penicillin) are ineffective against 

mycoplasmas, which lack a cell wall. 

Other antibiotics such as tylosin, neomycin, 

tetracycline, or gentamicin are 

only effective against a few mycoplasma 

species commonly found in cell cultures. 

In addition, they frequently just reduce 

the concentration of mycoplasmas rather 

than completely eliminating them. As 

soon as treatment is discontinued, contamination 

will recur. 

BIOMYC-1 and 2 

BIOMYC-1 and 2 are two antibiotics, 

which eliminate mycoplasmas very efficiently 

when used in combination. After 

a four-day treatment with BIOMYC-1, 

a three-day treatment with BIOMYC-2 

follows. This is repeated 2 to 3 times. 

Following this protocol, mycoplasmas 

will not develop resistance to these antibiotics 

- a common occurrence with 

other antibiotic treatment methods. 

BIOMYC-1 is based on the antibiotic tiamutin 

which is produced by the fungus 

Pleurotus mutilus. BIOMYC-2 is based 

on the antibiotic minocycline, an improved 

derivative of tetracycline. 

BIOMYC-3 

BIOMYC-3 is based on the antibiotic 

ciprofloxacin, which is a member of 

the fluoroquinolone group and inhibits 

synthesis of DNA gyrase, an enzyme 

necessary for bacterial DNA supercoiling. 

Many mycoplasma species are 

sensitive to BIOMYC-3, including M. 

orale, M. hyorhinis, M. fermentans, and 

M. arginini, and also Acheloplasma laidlawii. 

These species are responsible for 

most mycoplasma contaminations in cell 

cultures. No cytotoxic effects on the cells 

in culture have been found at the concentrations 

recommended for use, and 

the treatment can be performed within 

12 days. 

Fig. 3: Fluorescence microscope image of 

cells contaminated with mycoplasmas. 

Picture kindly provided by M. Ebert, 

Forschungszentrum für Medizintechnik 

und Biotechnologie, Erfurt, Germany. 



Mycoplasma-EX 3 treatments PK-CC91-4003 

BIOMYC-1 

BIOMYC-2 

BIOMYC-3 

10 ml 

20 ml 

100 ml 

10 ml 

20 ml 

100 ml 

10 ml 

20 ml 

100 ml 

PK-CC03-036-1D 

PK-CC03-036-1C 

PK-CC03-036-1B 

PK-CC03-037-1D 

PK-CC03-037-1C 

PK-CC03-037-1B 

PK-CC03-038-1D 

PK-CC03-038-1C 

PK-CC03-038-1B 

7 Microbial 

Detection and 

Elimination

112 

7 Microbial Detection and Elimination 

7.3 Bacteria Test Kit 

Applications 

Bacteria detection in cell culture 

Bacteria detection in virus stocks 

Bacteria detection in medium 

Features 

Highly sensitive (detects 12 bacteria 

genomes per sample volume) 

Reproducible and reliable results 

Applicable for 45 bacteria species 

Positive and internal controls included 

Convenient protocol and easy sample 

preparation 

Bacteria are frequently found as contaminants 

in cell cultures. However, cell 

cultures sometimes lack visual signs of 

the bacterial contamination, such as fast 

decoloration of the medium. In addition, 

it has been demonstrated that standard 

antibiotics are mostly ineffective against 

resistant bacterial infection but have 

a strong impact on the metabolism, 

growth, and differentiation of the cells 

in culture. 

Frequent screening of cell cultures for 

bacterial contamination is essential 

to ensure reliable results. Therefore, 

PromoKine offers a PCR-based Bacteria 

Test Kit that has been developed for 

accurate and reliable in vitro diagnosis of 

45 bacteria species, which are the common 

contaminants in cell cultures (e.g. 

Actinomyces, Bacillus, Enterococcus, 

Escherichia coli, Fusobacterium, Klebsiella, 

Lactobacillus, Micrococcus, Mycobacterium, 

Peptostreptococcus, Pseudomonas, 

Porphyromonas, Prevotella, 

Salmonella, Serratia, Staphylococcus, 

Streptococcus, etc.). The kit provides a 

highly sensitive, easy, and rapid method 

to detect bacterial infection in various 

biological materials, including cell cultures. 

Detection requires as little as 12 

bacteria genomes (~52 fg) per sample 

volume. The protocol can be completed 

within 3.5 hours with a hands-on time 

of less than 40 minutes. The kit contains 

all the necessary components for setting 

up a PCR including a positive control 

DNA as well as an internal control DNA 

to confirm a successfully performed PCR 

run. The kit does not amplify eukaryotic 

DNA. 

PCR 

200 - 500 µl cell 

culture supernatant 

Extract Bacterial 

DNA 

5 µl DNA sample 

Analyze 5 µl of 

the PCR sample 

Fig. 4: Bacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient: Purify bacterial DNA from 200 – 500 

µl of cell culture supernatant using an appropriate DNA extraction kit; add 5 µl sample DNA to the reaction cup with the premixed 

PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. 



PCR Bacteria Test Kit 24 tests PK-CA91-2024 

PCR Bacteria Test Kit 48 tests PK-CA91-2048 

PromoKine also offers a Bacteria Live/Dead Staining Kit as well as fluorescent dyes for staining of bacteria, please see Chapter 1 

of the PromoKine Catalog. 

7 Microbial 

Detection and 

Elimination


7.4 Disinfectants 

Disinfection of all tissue culture equipment 

and work surfaces in the lab has to 

be performed regularly to prevent contamination. 

PromoKine offers different 

disinfectants ideal for laboratory surfaces 

and apparatuses, CO 2 

incubators, and 

water baths. 



Mycoplasma-EX Spray 

Applications 

Disinfection of all laboratory surfaces 

Disinfection of laboratory apparatuses 

Features 

Kills mycoplasmas within seconds 

Effective against most microorganisms 

Non-corrosive and non-cancerogenic 

Does not leave any residue 

Supplied in a refillable spray bottle 

cidal activity, it is very effective against 

Staphylococcus aureus, Streptococcus 

faecalis, Escherichia coli, Proteus mirabilis, 

Pseudomonas aeruginosa, Candida 

albicans, Mycobacteria, HBV/HIV, Papova, 

Adeno, Polio and Vaccinia viruses. 

The effectiveness of Mycoplasma-EX 

Spray is further enhanced by its excellent 

detergent properties, achieving clean and 

contamination-free surfaces in one step. 

Pharmacidal Spray 

Applications 

Disinfection of incubators 

Features 

Free of mercury, formaldehyde, 

phenol, and alcohol 

Very effective against bacteria, fungi, 

spores, and viruses 

Non-corrosive and non-cancerogenic 

Does not leave any residue 

Supplied in a refillable spray bottle 

Pharmacidal Spray is a mercury-, formaldehyde-, 

phenol-, and alcohol-free 

disinfectant specifically designed for the 

disinfection of incubators. Spraying the 

incubators thoroughly every two weeks 

is recommended. 

Aquaguard 

Applications 

Disinfection of water trays in CO 2 

incubators 

Disinfection of water baths 

Features 

Very effective against bacteria, fungi, 

spores, and viruses 

Non-corrosive 

Non-toxic and non-volatile 

The water in an incubator or water bath 

is a major source of cell culture contamination, 

particularly with respect 

to mycoplasma contaminations, since 

mycoplasmas can survive in pure water. 

Aquaguard, a non-volatile disinfectant, 

is extremely effective at eliminating the 

microorganisms in water. In addition, it 

does not cause damage to the stainless 

steel of the incubator or any plastic interior, 

and it does not cause any skin irritation 

when used as recommended. 

Aquaguard-1 is intended for disinfecting 

water in incubators, whereas Aquaguard-2 

is intended for water bath disinfection. 

Mycoplasma-EX Spray allows safe disinfection 

and cleaning of all laboratory surfaces 

and apparatuses, including work 

benches, incubators, cell storage boxes, 

and liquid nitrogen containers. It utilizes 

the powerful Mycoplasma-EX reagent 

in combination with an alcohol-based 

solution. Besides its strong mycoplasmahttps://www.promokine.info/shop 


Mycoplasma-EX Spray 

Pharmacidal Spray 

Aquaguard-1 (incubator) 

Aquaguard-2 (water bath) 

Mycoplasma Disinfection Kit I 

(Consists of Mycoplasma-Ex Spray 500 ml, 

Aquaguard-1, and Aquaguard-2) 

Mycoplasma Disinfection Kit II 

(Consists of Pharmacidal Spray 1 L, 

Aquaguard-1, and Aquaguard-2) 

500 ml 

5 L 

1000 ml 

5 L 

100 ml 

10 x 100 ml 

50 ml 

10 x 50 ml 

PK-CC91-5050 

PK-CC91-5050-5L 

PK-CC-110100 

PK-CC-110100-5L 

PK-CC01-867-1B 

PK-CC01-867-10B 

PK-CC01-916-1E 

PK-CC01-916-10E 

1 Kit PK-CC91-5060 

1 Kit PK-CC91-5070 

7 Microbial 

Detection and 

Elimination

PromoKine 

8 

Cell Biology Products 

PromoKine – a brand of PromoCell - provides a wide range of 

state-of-the-art tools for cell biology research including many 

innovative reagents and kits for cell analysis, cell transfection, 

fluorescent labeling, gene cloning and expression, and mycoplasma 

detection and elimination. Also PromoKine offers 

a huge variety of recombinant proteins such as cytokines, 

growth factors, caspases, and kinases as well as ELISA kits, 

and primary and secondary antibodies.

116 


3 

8 

PromoKine - Cell Biology Products 

8.1 Cell Analysis ...................... 117 

8.2 Apoptosis ........................ 117 

8.3 Fluorescent Labeling ................ 117 

8.4 Gene Cloning, Expression, and Silencing . 118 

8.5 Cell Transfection ................... 118 

8.6 Antibodies and ELISAs. .............. 118 

8.7 Cytokines and Growth Factors. ........ 119

8 Cell Biology 117 

8.1 Cell Analysis 

8.2 Apoptosis 8.3 Fluorescent Labeling 

The specific analysis of cellular processes 

and conditions has played an increasingly 

vital role in cell biology. Monitoring 

reporter gene expression in cells, determining 

cell viability and proliferation, 

testing for cytotoxicity, and distinguishing 

between live and dead cells are important 

procedures in most cell biology 

labs. Measuring intracellular signaling 

and assaying distinct cell metabolic activities 

have also become routine. These 

applications all require reliable, sophisticated, 

and user-friendly research tools. 

Thus, PromoKine offers numerous reagents 

and kits for cell analysis, e.g. for 

detection and quantitation of cellular 

processes or staining of cells or cell structures: 

Cell Viability and Proliferation Kits 

Live/Dead Cell Staining Kits 

Cytotoxicity Kits 

Senescence Detection Kit 

Cell-Based Reporter Assays 

Cell Staining Reagents and Related 

Products 

Intracellular Indicators and Related 

Products 

Cell Signaling Assays 

Cell Stress and Metabolism Kits 

Cell Fractionation and Protein 

Extraction Kits 

Nucleic Acid Isolation Kits 

Apoptosis, or programmed cell death, is 

a sophisticated process that involves an 

intricate series of biochemical events. 

Specific cell death signals are transduced 

by different biomolecules such 

as caspases, which initiate an apoptotic 

cascade. This process culminates in multiple 

events such as degradation of cellular 

proteins and chromosomal DNA, 

mitochondrial disruption, and changes in 

plasma membrane integrity. 

For the reliable detection and quantification 

of different apoptotic events such 

as DNA fragmentation, mitochondria 

and plasma membrane changes as well 

as enzymatic activities of apoptosisspecific 

proteins, PromoKine provides 

an extensive line of user-friendly kits 

and reagents for highly sensitive colorimetric 

or fluorometric measurement: 

DNA Fragmentation Detection Kits 

Mitochondrial Apoptosis Staining Kit 

Cytochrome c Apoptosis Detection Kit 

Glutathione Detection Kits 

Annexin V Detection Kits 

Caspase Detection Kits 

Caspase Quantification Kits 

Cathepsin/Calpain Detection Kits 

Kinase Detection Kits 

Apoptotic and Necrotic Cell Detection 

Apoptotic Cell Isolation Kit 

Apoptosis Inducers 

The availability of new fluorophores has 

dramatically changed the possibilities for 

the sensitive detection of biomolecules 

and the analysis of their interactions. 

Improved fluorescent dyes are now enabling 

previously impossible studies of 

cellular structures and processes. 

Our PromoFluor dyes are cost-effective, 

high quality alternatives to commonly 

used, well-known fluorophores and span 

the entire visible wavelengths spectrum 

from blue to far red. 

PromoKine offers a wide choice of reactive 

fluorescent dyes for easy labeling of 

diverse biomolecules including proteins, 

antibodies, and DNA, as well as complete 

labeling kits and ready-to-use fluorescent 

conjugates: 

PromoFluor Reactive Dyes 

PromoFluor Protein and Antibody 

Labeling Kits 

PromoFluor DNA Labeling Kits 

PromoFluor Labeled Antibodies 

PromoFluor Labeled Phalloidin 

PromoFluor Labeled Nucleotides 

PromoFluor Labeled Biotin 

and Streptavidin 

Phycobiliprotein Dyes 

Phycobiliprotein Labeled Biotin and 

Streptavidin 

PromoFluor Antifade Reagent 

More information can be found in our PromoKine catalog or at the PromoKine website www.promokine.info 

Fig. 1: Double-staining of viable 

(green) and dead (red) cells using the 

PromoKine Live/Dead Cell Staining Kit II. 

Fig. 2: Apoptotic U937 cells stained with 

Annexin V-FITC using the PromoKine 

Apoptotic/Necrotic/Healthy Cells Detection 

Kit. 

Fig. 3: Cell structures of HeLa cells 

visualized using a primary anti-betatubulin 

antibody and a secondary 

PromoFluor 488-labeled antibody (green) 

as well as a primary anti-centromere antibody 

and a secondary PromoFluor 547- 

labeled antibody (red). 

8 Cell Biology

118 

8 Cell Biology 

8.4 Gene Cloning, 

Expression, and Silencing 

Manipulating DNA via cloning and PCR 

techniques facilitates complex genetic 

analysis. PCR has become a core technique 

in molecular biology and it enables 

a wide array of biological experiments 

and practical applications. Amplifying 

DNA has also allowed the study of gene 

expression, including the examination 

of genetic information transcribed and 

translated into RNA and proteins. In 

contrast, gene silencing has also grown 

into an important technique for analyzing 

gene functions. 

PromoKine provides a selection of vector 

systems allowing convenient cloning and 

high-level expression of specific target 

genes of interest as well as plasmid vectors 

expressing commonly used reporter 

genes or siRNA templates to generate 

specific siRNAs for gene silencing studies: 

pPK-CMV Expression Vectors 

pPK-CMV Reporter Vectors 

pPK-CMV Fusion Vectors 

StarGate Combinatorial Cloning 

System 

StarGate Mutagenesis System 

StarGate Fusion Cloning System 

GeneOFF shRNA Vector Kits 

GeneOFF siRNA Generation Kit 

8.5 Cell Transfection 

The ability to introduce foreign nucleic 

acids into eukaryotic cells has become a 

powerful tool to study and control gene 

expression. Cloned genes can be transfected 

into cells for biochemical characterization, 

mutational analyses, investigation 

of gene regulatory elements, and the 

production of specific proteins. Cell transfection 

promotes the rapid advancement 

of our knowledge of genetic regulation 

and protein function. 

PromoFectin Transfection Reagents provide 

highly efficient delivery of nucleic acids 

and proteins/peptides into a variety of 

adherent and non-adherent cell types, including 

many hard-to-transfect cell lines 

and primary cells. They show very low 

cytotoxicity and are therefore also ideally 

suited for transfection of very sensitive 

cells. PromoFectin variants optimized for 

transfection of HUVECs, hepatocytes, 

macrophages, neuronal, and insect cells 

are also available. 

In addition, the innovative Magnet-Assisted 

Transfection technology (MATra) 

provides another highly effective means 

for a very fast (transfection is completed 

within 15 – 20 minutes!) and successful 

delivery of nucleic acids into a variety of 

mammalian cells – also including many 

difficult-to-transfect cell types: 

PromoFectin Transfection Reagents 

Magnet-Assisted Transfection (MATra) 

Transfection Accessories 

8.6 Antibodies 

and ELISAs 

Antibodies have a broad range of applications 

like the detection of cellular 

structures as well as the specific identification, 

localization, and purification of 

intracellular and extracellular biomolecules 

such as proteins. They are also 

essential tools in describing cellular processes 

and for diagnostic purposes. Consequently, 

they are important for many 

research disciplines in cell biology. 

PromoKine offers a wide range of antibodies 

and ELISAs for different research 

fields such as cell signaling/signal transduction, 

apoptosis, neuroscience, virology, 

homeostasis, stem cell and cancer 

research. 

In addition, labeled secondary antibodies, 

antibody labeling kits as well as a variety 

of control cell lysates and blocking 

peptides are available: 

Mono- and Polyclonal Primary Antibodies 

Labeled Primary and Secondary 

Antibodies 

Antibody Labeling Kits 

Western Blotting Kits and Reagents 

Ready-to-use ELISA Kits 

ELISA Development Kits 

Antigens (Blocking Peptides) 

Cell Lysates 


Fig. 4: b-Gal staining of CHO-K1 cells 

Fig. 5: Primary neurons transfected with 

Fig. 6: PromoKine ELISA kits are dis- 

8 Cell Biology 

transfected with the PromoKine pPK- 

CMV-R1 reporter vector expressing the 

lacZ gene. 

the PromoKine reporter vector pPK- 

CMV-R4 (see chapter 4 of PromoKine 

Catalog) using PromoFectin-Neuron. 

tinguished by their high sensitivity and 

reproducibility as well as their userfriendly 

and convenient protocols.

8 Cell Biology 119 

8.7 Cytokines 

and Growth Factors 

Cytokines and chemokines are small proteins 

secreted by a variety of cell types. 

They are important signal messengers 

mediating cell communication and activating 

other target cells through their 

binding to specific receptors. 

Cytokines play a central role in the immune 

system - in which they mainly 

function as chemoattractants. They also 

coordinate and regulate other biological 

processes such as cell growth and development, 

or tissue repair. 

For many years, PromoKine has provided 

an exceptionally wide range of highquality 

recombinant proteins that were 

produced in E. coli, yeast, insect, plant 

or mammalian cells. They meet the highest 

quality standards with regard to their 

stability, purity, and biological activity. 

Our recombinant proteins are very useful 

for studying cellular processes such 

as cell differentiation and programming, 

angiogenesis and aging as well as diseases 

like obesity, diabetes, and cancer: 

Cytokines, Chemokines, 

and Growth Factors 

Hormones 

Animal-free Cytokines and 

Growth Factors 

Cell Culture-grade Cytokines 

Soluble Receptors and CD Antigens 

Cytokines and Growth Factors 

for Stem Cell Research 

Bulk sizes available at very 

economical prices 

OD (490 nm) 

1.6 

1.5 

Control fraction 

PromoKine´s animal-free VEGF-121 

Fig. 7: Cell proliferation assay using 

PromoKine´s animal-free VEGF-121 and HU- 

VEC cells (see page 11, Cat.No. C-12200). 

1.4 

1.3 

1.2 

0.1 

1.0 

0.9 

0.8 

0.7 

0.0 10-³ 10-² 10-¹ 1.0 10¹ 10² 

ng/ml VEGF-121 

10³ 


8 Cell Biology

120 

Alphabetical Index 


Description Page Description Page 

1 - 9 

3D-Angiogenesis Assay (HDMEC) 60 

3D-Angiogenesis Assay (HUVEC) 60 

Chondrocytes 10 

Chondrogenic Differentiation Medium 86 

Classical Media 90 

DMEM Media 90 

DMEM/Ham´s F-12 Media 90 

Accutase-Solution 101 

Adipocyte Media 83 

Adipogenic Differentiation Medium 86 

Airway Epithelial Cell Media 71 

Amphotericin B Solution 103 

Angiogenesis Assays and Kits 59 

Antibiotics and Antimycotics 103 

Antibodies and ELISAs 118 

Aortic Adventitial Fibroblasts 26 

Aortic Endothelial Cells 13 

Aortic Smooth Muscle Cells 35 

Apoptosis 117 

Aquaguard 113 

Bacteria Test Kit 112 

BIOMYC 111 

Bladder Microvascular Endothelial Cells 18 

Bladder Smooth Muscle Cells 38 

Blood Cells 46 

Blood Separation Medium 89 

Bovine Pituitary Extract 105 

Brain Microvascular Endothelial Cells 20 

Bronchial Epithelial Cells 22 

Bronchial Smooth Muscle Cells 38 

Buffers and Salt Solutions 104 

CaCl 2 

-Solution 104 

Cardiac Fibroblasts 26 

Cardiac Microvascular Endothelial Cells 18 

Cardiac Myocytes 9 

CD14 + Monocytes 53 

CD34 + Progenitor Cells 50 

CD133 + Progenitor Cells 51 

Cell Analysis 117 

Cell Biology Products 116 

Cell Dissociation Solution ACF 102 

Cell Model Systems 58 

Cell Pellets 97 

Cell Transfection 118 

A 

B 

C 

Ham´s F-10 Media 90 


Leibovitz L-15 Medium 90 

McCoy´s 5A Media 91 

MEM Media 91 

RPMI 1640 Media 91 

Collagen Type I Rat Tail 105 

Coronary Artery Endothelial Cells 13 

Coronary Artery Smooth Muscle Cells 36 

Cryo-SFM 85 

Cytokine Mix E 87 

Cytokines and Growth Factors 106 

DC Base Media 89 

DC Generation Media 89 

Dendritic Cell Media 88 

Dermal Blood Endothelial Cells 16 

Dermal Fibroblasts 25 

Dermal Lymphatic Endothelial Cells 17 

Dermal Microvascular Endothelial Cells 15 

DetachKit 1/2 101 

Detachment Reagents 101 

Disinfectants 113 

DMEM Media 90 

DNA Ladders 110 

Dulbecco’s PBS 104 

Endothelial Cell Growth Supplement 105 

Endothelial Cell Growth Supplement/Heparin 105 

Endothelial Cell Media 69 

Endothelial Cell Media MV 70 

Endothelial Cells (Large Vessels) 11 

Endothelial Cells (Microvascular) 15 

Endothelial Progenitor Medium 88 

Epidermal Keratinocytes 30 

Epidermal Melanocytes 31 

Epithelial Cell Media 71 

Epithelial Cells 21 

ESC-Qualified Fetal Calf Serum 93 

Extracellular Matrix Proteins 105 

D 

E 

Chondrocyte Media 68

Alphabetical Index 121 


Fetal Calf Serum 92 

Fibroblast Media 76 

Fibroblasts 25 

Fibronectin Solutions 105 

Fluorescent Labeling 117 

Follicle Dermal Papilla Cell Media 78 

Follicle Dermal Papilla Cells 28 

Freezing Medium 85 

F 

HMEpC 23 

hMNC-CB 55 

hMNC-PB 55 

hMoCD14 + -PB 53 

hMo-PB 53 

hMSC-AT 48 

hMSC-BM 48 

hMSC-UC 48 

HNEpC 21 

HOB 32 

HPAEC 14 

G-418 Sulphate Solution 103 

Gene Cloning, Expression, and Silencing 118 

Gentamicin Solution 103 

Glutamine, L 105 

Glutamine, Stable 105 



Hanks’ BSS 104 

HAoAF 26 

HAoEC 13 

HAoSMC 35 

HBdMEC 18 

HBdSMC 38 

HBEpC 22 

HBSMC 38 

HBMEC 20 

HCAEC 13 

HCASMC 36 

hCD34 + -CB 50 

hCD133 + -CB 51 

HCF 26 

HCH 10 

HCM 9 

HCMEC 18 

HDBEC 16 

HDLEC 17 

HDMEC 15 

Hematopoietic Progenitor Cell Media 87 

Hematopoietic Progenitor Cell Expansion Medium DXF 87 

Hepatocyte Media 79 

Hepatocytes 29 

HEPES Buffer 104 

HEPES Buffered Saline Solution 104 

HFDPC 28 

HHpC 29 

G 

H 

HPASMC 36 

hPC-PL 49 

HPF 26 

HPlEpC 24 

HPMEC 19 

HPrSMC 39 

HRCEpC 24 

HREpC 24 

HSAEpC 22 

HSaVEC 14 

HTEpC 22 

HTSMC 37 

HUAEC 12 

HUASMC 37 

HUF 27 

Human Insulin 105 

Human Primary Cells 8 

Cardiac Myocytes 9 

Chondrocytes 10 

Endothelial Cells (Large Vessels) 11 

Endothelial Cells (Microvascular) 15 

Epithelial Cells 21 

Fibroblasts 25 

Follicle Dermal Papilla Cells 28 

Hepatocytes 29 

Keratinocytes 30 

Melanocytes 31 

Osteoblasts 32 

Preadipocytes 33 

Skeletal Muscle Cells 34 

Smooth Muscle Cells 35 

Human Stem and Blood Cells 46 

Human Transferrin 105 

HUtMEC 19 

HUtSMC 39 

HUVEC 12 

HVMF 27 

HWP 33 

Hygromycin B Solution 103

122 



I, K, L 

Insect Cell Culture Media 92 

Insulin 105 

Invasion Kit (HDMEC) 63 

Invasion Kit (HUVEC) 63 

Keratinocyte Media 80 

Keratinocytes 30 

Leibovitz L-15 Medium 90 

L-Glutamine Solution 105 

Lymphatic Endothelial Cells 17 

Lymphocyte Separation Medium 1077 89 

Mesenchymal Stem Cell Chondrogenic Differentiation Medium 86 

Mesenchymal Stem Cell Growth Medium 86 

Mesenchymal Stem Cell Neurogenic Differentiation Medium 86 

Mesenchymal Stem Cell Osteogenic Differentiation Medium 86 

Mesenchymal Stem Cells 47 

Mesenchymal Stem Cells from Adipose Tissue 48 

Mesenchymal Stem Cells from Bone Marrow 48 

Mesenchymal Stem Cells from Umbilical Cord Matrix 48 

Microbial Detection and Elimination 107 

Mineralization Medium Osteoblasts 82 

Mitsuhashi/Maramorosh Insect Medium 92 

Monocytes 52 

Mononuclear Cell Medium 89 

M 

Mammary Epithelial Cell Media 73 

Mammary Epithelial Cells 23 

McCoy´s 5A Media 91 

Media and Sera 66 

Media for Primary Cells 67 

Chondrocyte Media 68 

Mononuclear Cells 54 

Mononuclear Cells from Cord Blood 55 

Mononuclear Cells from Peripheral Blood 55 

MSC-Qualified Fetal Calf Serum 93 

Mycoplasma Elimination Solutions 110 

Mycoplasma Test Kits 109 

Mycoplasma-EX Spray 113 

Myocyte Media 68 

Endothelial Cell Media 69 

Endothelial Progenitor Medium 88 

Epithelial Cell Media 71 

Fibroblast Media 76 

Follicle Dermal Papilla Cell Media 78 

Hematopoietic Progenitor Cell Media 87 

Hepatocyte Media 79 

Keratinocyte Media 80 

Melanocyte Media 81 

Mesenchymal Stem Cell Differentiation Media 86 

Mesenchymal Stem Cell Media 86 

Mesenchymal Stem Cell Growth Medium 86 

Mesenchymal Stem Cell Growth Medium DXF 86 

Monocyte Attachment Medium 89 

Mononuclear Cell Media 89 

Myocyte Media 68 

Osteoblast Media 82 

Pericyte Media 87 

Preadipocyte/Adipocyte Media 83 

Skeletal Muscle Cell Media 84 

Smooth Muscle Cell Media 85 

Melanocyte Media 81 

Melanocytes 31 

MEM Alpha Media 91 

MEM Amino Acids Solution 105 

MEM D-Valine, w/o L-Glutamine 91 

MEM Media 91 

MEM NEAA Solution 105 

MEM Vitamin Solution 105 

Mesenchymal Stem Cell Adipogenic Differentiation Medium 86 

N, O 

Nasal Epithelial Cells 21 

Neurogenic Differentiation Medium 86 

NHDF 25 

NHEK 30 

NHEM 31 

Osteoblasts 32 

Osteoblast Media 82 

Osteoblast Mineralization Medium 82 

Osteogenic Differentiation Medium 86 

Overview Human Primary Cells 40 

Overview Human Stem and Blood Cells 56 

PBS 104 

PCR Bacteria Test Kit 112 

PCR Mycoplasma Test Kits 109 

Pellets 97 

Pen/Strep Solution 103 

Pen/Strep/Fungizone Solution 103 

Pericytes 49 

Pericyte Media 87 

Pharmacidal Spray 113 

Placental Epithelial Cell Media 75 

Placental Epithelial Cells 24 

Planar Migration Assay (HDMEC) 61 

Planar Migration Assay (HUVEC) 61 

P

Alphabetical Index 123 


Preadipocyte Media 83 

Preadipocytes 33 

Primary Cells 8 

PromoKine Cell Biology Products 116 

Prostate Smooth Muscle Cells 39 

Pulmonary Artery Endothelial Cells 14 

Pulmonary Artery Smooth Muscle Cells 36 

Pulmonary Fibroblasts 26 

Pulmonary Microvascular Endothelial Cells 19 

U, V, W 

Umbilical Artery Endothelial Cells 12 

Umbilical Artery Smooth Muscle Cells 37 

Umbilical Vein Endothelial Cells 12 

Uterine Fibroblasts 27 

Uterine Microvascular Endothelial Cells 19 

Uterine Smooth Muscle Cells 39 

Villous Mesenchymal Fibroblasts 27 

White Preadipocytes 33 

R 

Reagents and Supplements 100 

Renal Cortical Epithelial Cells 24 

Renal Epithelial Cell Media 74 

Renal Epithelial Cells 24 

RPMI 1640 Media 91 

Saphenous Vein Endothelial Cells 14 

Schneider’s Drosophila Medium 92 

Sera 92 

Size Markers 110 

Skeletal Muscle Cell Media 84 

Skeletal Muscle Cells 34 

SkMC 34 

Small Airway Epithelial Cell Media 72 

Small Airway Epithelial Cells 22 

Smooth Muscle Cell Media 85 

Smooth Muscle Cells 35 

Sodium bicarbonate Solution 104 

Stable Glutamine Solution 105 

Stem and Blood Cells 46 

Supplements 107 

S 

TC Water 104 

Trace Element Mix 105 

Tracheal Epithelial Cells 22 

Tracheal Smooth Muscle Cells 37 

Transferrin 105 

Transmigration Kit (HDMEC) 62 

Transmigration Kit (HUVEC) 62 

Trypsin Neutralizing Solution (TNS) 102 

Trypsin Solutions 102 

T

124 

Numerical Index 


Cat. Number Product Page Cat. Number Product Page 

C-12000 – C-12499 

C-12255 Human Umbilical Vein 

Endothelial Cells, pre-screened 

12 

C-12001 Normal Human Epidermal Keratinocytes, 





adult donor 


adult donor 









C-12071 Human Follicle Dermal Papilla Cells 28 

C-12072 Human Follicle Dermal Papilla Cells 28 


Endothelial Cells, single donor 

C-12202 Human Umbilical Artery 

Endothelial Cells 


Endothelial Cells, pooled 



C-12210 Human Dermal Microvascular 

Endothelial Cells, juvenile foreskin 

C-12211 Human Dermal Blood 



Endothelial Cells, adult donor 





C-12216 Human Dermal Lymphatic 


C-12217 Human Lymphatic 


C-12218 Human Dermal Lymphatic 


C-12219 Human Lymphatic 


C-12221 Human Coronary Artery Endothelial Cells 13 

C-12222 Human Coronary Artery Endothelial Cells 13 





C-12231 Human Saphenous Vein Endothelial Cells 14 

C-12232 Human Saphenous Vein Endothelial Cells 14 

C-12241 Human Pulmonary Artery Endothelial Cells 14 

C-12242 Human Pulmonary Artery Endothelial Cells 14 


Endothelial Cells, single donor 




Endothelial Cells, pooled 

30 

30 

30 

30 

30 

30 

30 

30 

12 

12 

12 

12 

15 

16 

15 

16 

15 

17 

17 

17 

17 

16 

16 

12 

12 

12 







C-12271 Human Aortic Endothelial Cells 13 

C-12272 Human Aortic Endothelial Cells 13 

C-12281 Human Pulmonary Microvascular Endothelial 


C-12282 Human Pulmonary Microvascular Endothelial 


C-12285 Human Cardiac Microvascular 


C-12286 Human Cardiac Microvascular 


C-12287 Human Brain Microvascular 


C-12288 Human Brain Microvascular 


C-12291 Human Bladder Microvascular 


C-12292 Human Bladder Microvascular 


C-12295 Human Uterine Microvascular 


C-12296 Human Uterine Microvascular 


C-12300 Normal Human Dermal Fibroblasts, 


C-12302 Human Dermal Fibroblasts, 

adult donor 

C-12350 Normal Human Dermal Fibroblasts, 


C-12352 Human Dermal Fibroblasts, adult donor 25 

C-12360 Human Pulmonary Fibroblasts 26 

C-12361 Human Pulmonary Fibroblasts 26 

C-12375 Human Cardiac Fibroblasts 26 

C-12377 Human Cardiac Fibroblasts 26 

C-12380 Human Aortic Adventitial Fibroblasts 26 

C-12381 Human Aortic Adventitial Fibroblasts 26 

C-12385 Human Uterine Fibroblasts 27 

C-12386 Human Uterine Fibroblasts 27 

C-12390 Human Villous Mesenchymal Fibroblasts 27 

C-12391 Human Villous Mesenchymal Fibroblasts 27 

C-12395 Human Placental Epithelial Cells 24 

C-12397 Human Placental Epithelial Cells 24 

C-12400 Normal Human Epidermal Melanocytes, 



juvenile foreskin, cultured in M2 medium 


adult donor, cultured in M2 medium 




juvenile foreskin, cultured in M2 medium 


adult donor, cultured in M2 medium 

15 

15 

15 

19 

19 

18 

18 

20 

20 

18 

18 

19 

19 

25 

25 

25 

31 

31 

31 

31 

31 

31

Numerical Index 125 


C-12500 – C-19999 

C-12901 Human Mononuclear Cells 

from Cord Blood, single donor, ultra-pure 

55 



C-12511 Human Coronary Artery 


C-12512 Human Coronary Artery 


C-12521 Human Pulmonary Artery 


C-12522 Human Pulmonary Artery 


C-12530 Human Skeletal Muscle Cells 34 

C-12532 Human Aortic Smooth Muscle Cells 35 

C-12533 Human Aortic Smooth Muscle Cells 35 



C-12561 Human Bronchial Smooth Muscle Cells 38 

C-12562 Human Bronchial Smooth Muscle Cells 38 

C-12565 Human Tracheal Smooth Muscle Cells 37 

C-12566 Human Tracheal Smooth Muscle Cells 37 

C-12571 Human Bladder Smooth Muscle Cells 38 

C-12572 Human Bladder Smooth Muscle Cells 38 

C-12573 Human Prostate Smooth Muscle Cells 39 

C-12574 Human Prostate Smooth Muscle Cells 39 

C-12575 Human Uterine Smooth Muscle Cells 39 

C-12576 Human Uterine Smooth Muscle Cells 39 

C-12580 Human Skeletal Muscle Cells 34 

C-12620 Human Nasal Epithelial Cells 21 

C-12621 Human Nasal Epithelial Cells 21 

C-12640 Human Bronchial Epithelial Cells 22 

C-12641 Human Bronchial Epithelial Cells 22 

C-12642 Human Small Airway Epithelial Cells 22 

C-12643 Human Small Airway Epithelial Cells 22 

C-12644 Human Tracheal Epithelial Cells 22 

C-12645 Human Tracheal Epithelial Cells 22 

C-12650 Human Mammary Epithelial Cells 23 

C-12651 Human Mammary Epithelial Cells 23 

C-12660 Human Renal Cortical Epithelial Cells 24 

C-12662 Human Renal Cortical Epithelial Cells 24 

C-12665 Human Renal Epithelial Cells 24 

C-12666 Human Renal Epithelial Cells 24 

C-12710 Human Chondrocytes 10 

C-12720 Human Osteoblasts 32 

C-12730 Human White Preadipocytes, subcutaneus 33 

C-12731 Human White Preadipocytes, subcutaneus 33 

C-12732 Human White Preadipocytes, visceral 33 

C-12733 Human White Preadipocytes, visceral 33 

C-12750 Human Chondrocytes 10 

C-12760 Human Osteoblasts 32 

C-12810 Human Cardiac Myocytes 9 

C-12811 Human Cardiac Myocytes 9 

C-12850 Human Hepatocytes 29 

37 

36 

36 

36 

36 

37 


from Cord Blood, pooled, ultra-pure 


from Peripheral Blood, single donor, 

ultra-pure 


from Peripheral Blood, pooled, ultra-pure 

C-12909 Human CD14 + Monocytes from 

Peripheral Blood, single donor 

C-12910 Human Monocytes from 

Peripheral Blood, single donor 

C-12912 Human CD14 + Monocytes from 

Peripheral Blood, single donor, pre-screened 

C-12913 Human Monocytes from 

Peripheral Blood, single donor, pre-screened 

C-12921 Human CD34 + Progenitor Cells 

from Cord Blood, single donor 


from Cord Blood, pooled 


from Cord Blood single donor 


from Cord Blood pooled 

C-12971 Human Mesenchymal Stem Cells 





from Bone Marrow 


from Bone Marrow 


from Adipose Tissue 


from Adipose Tissue 

C-12980 Human Pericytes from Placenta 49 

C-12981 Human Pericytes from Placenta 49 

C-13010 3D-Angiogenesis Assay (HUVEC) 60 



C-13020 3D-Angiogenesis Assay (HDMEC) 60 



C-13030 Planar Migration Assay (HUVEC) 61 

C-13031 Planar Migration Assay (HDMEC) 61 

C-13035 Transmigration Kit (HUVEC) 62 

C-13036 Transmigration Kit (HDMEC) 62 

C-13040 Invasion Kit (HUVEC) 63 

C-13041 Invasion Kit (HDMEC) 63 

C-14001 NHEK.f Pellet 98 

C-14002 NHEK adult Pellet 98 

C-14003 NHEK. f pooled Pellet 98 

C-14004 NHEK adult pooled Pellet 98 

C-14005 HFDPC Pellet 97 

C-14010 HUVEC Pellet 98 

C-14011 HUVEC pooled Pellet 98 

55 

55 

55 

53 

53 

53 

53 

50 

50 

51 

51 

48 

48 

48 

48 

48 

48

126 



C-14012 HUVEC pre-screened Pellet 98 

C-14013 HUAEC Pellet 98 

C-14015 HDMEC Pellet 97 

C-14016 HDMEC adult Pellet 97 

C-14017 HDMEC pre-screened Pellet 97 

C-14018 HDBEC Pellet 97 

C-14019 HDBEC adult Pellet 97 

C-14020 HDLEC Pellet 97 

C-14021 HDLEC adult Pellet 97 

C-14022 HCAEC Pellet 97 

C-14023 HAoEC Pellet 97 

C-14024 HPAEC Pellet 98 

C-14025 HSaVEC Pellet 98 

C-14026 HBdMEC Pellet 97 

C-14027 HPMEC Pellet 98 

C-14028 HUtMEC Pellet 98 

C-14029 HCMEC Pellet 97 

C-14030 NHDF Pellet 98 

C-14031 NHDF adult Pellet 98 

C-14032 HBMEC Pellet 97 

C-14034 HVMF Pellet 98 

C-14035 HPF Pellet 98 

C-14036 HCF Pellet 97 

C-14037 HAoAF Pellet 97 

C-14038 HUF Pellet 98 

C-14040 NHEM.f Pellet 98 

C-14042 NHEM.f M2 Pellet 98 

C-14043 NHEM M2 adult Pellet 98 

C-14050 HUASMC Pellet 98 

C-14051 HPASMC Pellet 98 

C-14052 HCASMC Pellet 97 

C-14053 HAoSMC Pellet 97 

C-14055 HBSMC Pellet 97 

C-14056 HTSMC Pellet 98 

C-14057 HBdSMC Pellet 97 

C-14058 HPrSMC Pellet 98 

C-14059 HUtSMC Pellet 98 

C-14060 SkMC Pellet 98 

C-14062 HNEpC Pellet 98 

C-14063 HBEpC Pellet 97 

C-14064 HTEpC Pellet 98 

C-14065 HSAEpC Pellet 98 

C-14066 HMEpC Pellet 97 

C-14067 HREpC Pellet 98 

C-14068 HRCEpC Pellet 98 

C-14069 HPlEpC Pellet 98 

C-14070 HCH Pellet 97 

C-14071 HOB Pellet 98 

C-14072 HWP subcutaneous Pellet 98 

C-14073 HWP visceral Pellet 98 

C-14080 HCM Pellet 97 

C-14085 HHpC Pellet 97 

C-14090 hMSC-BM Pellet 98 

C-14091 hMSC-UC Pellet 98 

C-14092 hMSC-AT Pellet 98 

C-14095 hPC-PL Pellet 98 

C-14096 hMNC-CB single donor Pellet 97 

C-14097 hMNC-CB pooled Pellet 97 

C-14098 hMNC-PB single donor Pellet 97 

C-14099 hMNC-PB pooled Pellet 97 

C-14110 hMo-CD14 + -PB single donor Pellet 98 

C-14111 hMo-PB single donor Pellet 98 

C-14112 hMo-CD14 + -PB single donor 


C-14113 hMo-PB single donor pre-screened Pellet 98 

C-20000 – C-24999 

C-20011 Keratinocyte Growth Medium 2 80 

C-20111 Keratinocyte Growth Medium 2 Kit 80 

C-20211 Keratinocyte Basal Medium 2 80 

C-20216 Keratinocyte Basal Medium 2, 


C-21010 Mammary Epithelial Cell Growth Medium 73 

C-21060 Airway Epithelial Cell Growth Medium 71 

C-21070 Small Airway Epithelial Cell 

Growth Medium 

C-21110 Mammary Epithelial Cell 

Growth Medium Kit 

C-21160 Airway Epithelial Cell Growth Medium Kit 71 

C-21170 Small Airway Epithelial Cell 


C-21210 Mammary Epithelial Cell Basal Medium 73 

C-21215 Mammary Epithelial Cell Basal Medium, 


C-21260 Airway Epithelial Cell Basal Medium 71 

C-21265 Airway Epithelial Cell Basal Medium, 


C-21270 Small Airway Epithelial Cell Basal Medium 72 

C-21275 Small Airway Epithelial Cell Basal Medium, 


C-22010 Endothelial Cell Growth Medium 69 

C-22011 Endothelial Cell Growth Medium 2 69 

C-22020 Endothelial Cell Growth Medium MV 70 

C-22022 Endothelial Cell Growth Medium MV 2 70 

C-22062 Smooth Muscle Cell Growth Medium 2 85 

C-22070 Myocyte Growth Medium 68 

C-22110 Endothelial Cell Growth Medium Kit 69 

C-22111 Endothelial Cell Growth Medium 2 Kit 69 

C-22120 Endothelial Cell Growth Medium MV Kit 70 

C-22121 Endothelial Cell Growth Medium MV 2 Kit 70 

C-22162 Smooth Muscle Cell Growth Medium 2 Kit 85 

C-22170 Myocyte Growth Medium Kit 68 

C-22210 Endothelial Cell Basal Medium 69 

98 

80 

72 

73 

72 

73 

71 

72



C-22211 Endothelial Cell Basal Medium 2 69 

C-22215 Endothelial Cell Basal Medium, 


C-22216 Endothelial Cell Basal Medium 2, 


C-22220 Endothelial Cell Basal Medium MV 70 

C-22221 Endothelial Cell Basal Medium MV 2 70 

C-22225 Endothelial Cell Basal Medium MV, 


C-22226 Endothelial Cell Basal Medium MV 2, 


C-22262 Smooth Muscle Cell Basal Medium 2 85 

C-22267 Smooth Muscle Cell Basal Medium 2, 


C-22270 Myocyte Basal Medium 68 

C-22275 Myocyte Basal Medium, 


C-23010 Fibroblast Growth Medium 76 

C-23020 Fibroblast Growth Medium 2 77 

C-23025 Fibroblast Growth Medium 3 77 

C-23060 Skeletal Muscle Cell Growth Medium 84 

C-23061 Skeletal Muscle Cell Differentiation 

Medium 

C-23110 Fibroblast Growth Medium Kit 76 

C-23120 Fibroblast Growth Medium 2 Kit 77 

C-23130 Fibroblast Growth Medium 3 Kit 77 

C-23160 Skeletal Muscle Cell Growth Medium Kit 84 

C-23210 Fibroblast Basal Medium 76 

C-23215 Fibroblast Basal Medium, phenol red-free 76 

C-23220 Fibroblast Basal Medium 2 77 

C-23225 Fibroblast Basal Medium 2, phenol red-free 77 

C-23230 Fibroblast Basal Medium 3 77 

C-23235 Fibroblast Basal Medium 3, phenol red-free 77 

C-23260 Skeletal Muscle Cell Basal Medium 84 

C-23265 Skeletal Muscle Cell Basal Medium, 


C-24010 Melanocyte Growth Medium 81 

C-24110 Melanocyte Growth Medium Kit 81 

C-24210 Melanocyte Basal Medium 81 

C-24215 Melanocyte Basal Medium, phenol red-free 81 

C-24300 Melanocyte Growth Medium M2 81 

C-24305 Melanocyte Growth Medium M2, 


C-24400 Melanocyte M2 Basal Medium 81 

C-24405 Melanocyte M2 Basal Medium, 


C-25000 – C-29999 

C-25010 Hepatocyte Growth Medium 79 

C-25020 Hepatocyte Maintenance Medium 79 

C-25110 Hepatocyte Growth Medium Kit 79 

C-25120 Hepatocyte Maintenance Medium Kit 79 

C-25210 Hepatocyte Basal Medium 79 

C-25215 Hepatocyte Basal Medium, phenol red-free 79 

69 

69 

70 

70 

85 

68 

84 

84 

81 

81 

C-26001 Renal Epithelial Cell Growth Medium 74 

C-26011 Renal Epithelial Cell Growth Medium Kit 74 

C-26021 Renal Epithelial Cell Basal Medium 74 

C-26022 Renal Epithelial Cell Basal Medium, 


C-26030 Renal Epithelial Cell Growth Medium 2 74 

C-26040 Placental Epithelial Cell Growth Medium 75 

C-26130 Renal Epithelial Cell Growth Medium 2 Kit 74 

C-26140 Placental Epithelial Cell Growth Medium Kit 75 

C-26230 Renal Epithelial Cell Basal Medium 2 74 

C-26235 Renal Epithelial Cell Basal Medium 2, 


C-26240 Placental Epithelial Cell Basal Medium 75 

C-26245 Placental Epithelial Cell Basal Medium, 


C-26500 Follicle Dermal Papilla Cell Basal Medium 78 

C-26501 Follicle Dermal Papilla Cell Growth Medium 78 

C-26502 Follicle Dermal Papilla Cell 


C-26505 Follicle Dermal Papilla Cell Basal Medium, 


C-27001 Osteoblast Growth Medium 82 

C-27010 Osteoblast Basal Medium 82 

C-27015 Osteoblast Basal Medium, phenol red-free 82 

C-27020 Osteoblast Mineralization Medium 82 

C-27101 Chondrocyte Growth Medium 68 

C-27111 Chondrocyte Basal Medium 68 

C-27115 Chondrocyte Basal Medium, 


C-27410 Preadipocyte Growth Medium 83 

C-27411 Preadipocyte Basal Medium 83 

C-27415 Preadipocyte Basal Medium, 


C-27417 Preadipocyte Growth Medium Kit 83 

C-27431 Adipocyte Basal Medium 83 

C-27435 Adipocyte Basal Medium, phenol red-free 83 

C-27436 Preadipocyte Differentiation Medium 83 

C-27437 Preadipocyte Differentiation Medium Kit 83 

C-27438 Adipocyte Nutrition Medium 83 

C-27439 Adipocyte Nutrition Medium Kit 83 

C-28010 Mesenchymal Stem Cell Growth Medium 86 

C-28011 Mesenchymal Stem Cell Adipogenic 


C-28012 Mesenchymal Stem Cell Chondrogenic 


C-28013 Mesenchymal Stem Cell Osteogenic 


C-28014 Mesenchymal Stem Cell Chondrogenic 


C-28015 Mesenchymal Stem Cell Neurogenic 


C-28019 Mesenchymal Stem Cell 

Growth Medium DXF 

C-28020 Hematopoietic Progenitor Cell Medium 87 

C-28021 Hematopoietic Progenitor Cell Expansion 

Medium DXF 

C-28025 Endothelial Progenitor Medium 88 

74 

74 

75 

78 

78 

68 

83 

86 

86 

86 

86 

86 

86 

87

128 



C-28030 Mononuclear Cell Medium 89 

C-28040 Pericyte Growth Medium 87 

C-28050 DC Generation Medium 89 

C-28051 Monocyte Attachment Medium 89 

C-28052 DC Generation Medium DXF 89 

C-28053 DC Base Medium 89 

C-28054 DC Base Medium DXF 89 

C-29910 Cryo-SFM 85 

C-29912 Cryo-SFM 85 

C-39220 Endothelial Cell Growth Medium MV 




C-39225 Endothelial Cell Growth Medium MV 

Supplement Mix 



C-39262 Smooth Muscle Cell Growth Medium 2 


C-39267 Smooth Muscle Cell Growth Medium 2 


70 

70 

70 

70 

85 

85 

C-30000 – C-39999 

C-30020 Bovine Pituitary Extract 15 105 




C-30120 Endothelial Cell Growth Supplement/ 

Heparin 

C-30140 Endothelial Cell Growth Supplement/ 

Heparin 

105 

105 

C-30160 Endothelial Cell Growth Supplement 105 

C-30180 Endothelial Cell Growth Supplement 105 

C-34006 CaCl 2 

-Solution (0.5 M) 104 

C-37350 Fetal Calf Serum (EU Quality) 

human cell culture tested 


human cell culture tested 


Mesenchymal Stem Cell Qualified 


Mesenchymal Stem Cell Qualified 


Embryonic Stem Cell qualified 


Embryonic Stem Cell qualified 

C-39011 Keratinocyte Growth Medium 2 


C-39016 Keratinocyte Growth Medium 2 


C-39110 Mammary Epithelial Cell Growth Medium 


C-39115 Mammary Epithelial Cell Growth Medium 


C-39160 Airway Epithelial Cell Growth Medium 


C-39165 Airway Epithelial Cell Growth Medium 


C-39170 Small Airway Epithelial Cell Growth 


C-39175 Small Airway Epithelial Cell Growth 

Medium Supplement Mix 

C-39210 Endothelial Cell Growth Medium 




C-39215 Endothelial Cell Growth Medium 




92 

92 

93 

93 

93 

93 

80 

80 

73 

73 

71 

71 

72 

72 

69 

69 

69 

69 

C-39270 Myocyte Growth Medium 


C-39275 Myocyte Growth Medium Supplement Mix 68 

C-39310 Fibroblast Growth Medium 


C-39315 Fibroblast Growth Medium 










C-39360 Skeletal Muscle Cell Growth Medium 


C-39365 Skeletal Muscle Cell Growth Medium 


C-39366 Skeletal Muscle Cell Differentiation 

Medium Supplement Mix 

C-39410 Melanocyte Growth Medium 


C-39415 Melanocyte Growth Medium 


C-39420 Melanocyte Growth Medium M2 


C-39425 Preadipocyte Growth Medium 


C-39427 Preadipocyte Growth Medium 


C-39436 Preadipocyte Differentiation Medium 


C-39437 Preadipocyte Differentiation Medium 


C-39438 Adipocyte Nutrition Medium 


C-39439 Adipocyte Nutrition Medium 


C-39600 Renal Epithelial Cell Growth Medium 


C-39604 Renal Epithelial Cell Growth Medium 






C-39610 Placental Epithelial Cell Growth Medium 


C-39612 Placental Epithelial Cell Growth Medium 


C-39615 Osteoblast Growth Medium 


68 

76 

76 

77 

77 

77 

77 

84 

84 

84 

81 

81 

81 

83 

83 

83 

83 

83 

83 

74 

74 

74 

74 

75 

75 

82



C-39620 Follicle Dermal Papilla Cell Growth Medium 


C-39625 Follicle Dermal Papilla Cell Growth Medium 


C-39635 Chondrocyte Growth Medium 


C-39640 Hepatocyte Growth Medium 


C-39642 Hepatocyte Growth Medium 


C-39650 Hepatocyte Maintenance Medium 


C-39652 Hepatocyte Maintenance Medium 


C-39890 Cytokine Mix E for HPC-Expansion 

Medium DXF 

C-39891 Cytokine Mix E for HPC-Expansion 

Medium DXF 

C-40000 – C-49999 

78 

78 

68 

79 

79 

79 

79 

87 

87 

C-41222 DetachKit 2-250 101 

C-41310 Accutase-Solution, 


101 

C-41320 Cell Dissociation Solution ACF 102 

C-42010 Pen/Strep Solution 103 

C-42020 Pen/Strep/Fungizone Solution 103 

C-42040 Amphotericin B Solution 103 

C-42060 Gentamicin Solution 103 

C-42094 G-418 Sulphate Solution 103 

C-42095 Hygromycin B Solution 103 

C-42210 L-Glutamine Solution 105 

C-42215 Stable Glutamine Solution 105 

C-42320 MEM Amino Acids Solution 105 

C-42322 MEM NEAA Solution 105 

C-42328 MEM Vitamin Solution 105 

C-42331 Trace Element Mix (1000x) 105 

C-42340 Sodium Bi-Carbonate Solution 104 

C-43010 Collagen Type I Rat Tail 105 

C-40000 HEPES Buffered Saline Solution 104 



C-40030 HEPES Buffer (1 M) 104 

C-40230 Dulbecco's PBS 104 

C-43020 Collagen Type I Rat Tail 105 

C-43050 Fibronectin Solution Bovine 105 

C-43060 Fibronectin Solution Human 105 

C-44010 Lymphocyte Separation Medium 1077 89 

C-49998 TC Water 104 

C-40232 Dulbecco's PBS, w/o Ca ++ / Mg ++ 104 

C-40238 Dulbecco's PBS, w/o Ca ++ /Mg ++ , 10x conc. 104 

C-40240 PBS, endotoxin free 104 

C-40242 PBS, w/o Ca ++ /Mg ++ , endotoxin free 104 

C-40310 Hanks' BSS 104 

C-40320 Hanks' BSS, w/o Ca ++ /Mg ++ 104 

C-40370 Hanks' BSS, phenol red-free 104 

C-40390 Hanks' BSS, w/o Ca ++ /Mg ++ , phenol 

red-free 

104 

C-41000 Trypsin / EDTA 102 

C-41002 Trypsin / EDTA 2 102 





C-41040 Trypsin PBS 1:250, w/o Ca ++ /Mg ++ 102 

C-41050 Trypsin PBS 1:250, w/o Ca ++ /Mg ++ , 10x 

conc., CC grade 

102 

C-41060 Trypsin-EDTA PBS 1:250 102 

C-41070 Trypsin-EDTA PBS 1:250, w/o Ca ++ /Mg ++ , 

10x conc., CC grade 

102 

C-41100 Trypsin Neutralizing Solution 102 



C-41200 DetachKit-30 101 

C-41202 DetachKit 2-30 101 

C-50000 – C-69999 

C-52310 Human Insulin, human cell culture tested 105 

C-52322 Human Transferrin APO, low iron content 105 

C-52332 Human Transferrin HOLO, iron saturated 105 

C-60170 EGF, human, recombinant (E. coli) 106 

C-60182 EGF, human, recombinant (Pichia) 106 

C-60212 Insulin, human, recombinant (E. coli) 106 

C-60240 FGF-2 (FGF-b), human, recombinant (E. coli) 106 

C-60340 FGF-1 (FGF-a), human, recombinant (E. coli) 106 

C-60420 GM-CSF, human, recombinant (E. coli) 106 

C-60434 G-CSF, human, recombinant (E. coli) 106 

C-60442 M-CSF, human, recombinant (E. coli) 106 

C-61301 IL-3 CC, human, recombinant (E. coli) 106 



C-63120 SCF, human, recombinant (E. coli) 106 

C-63200 sRANKL, human recombinant (E. coli) 106 

C-63722 TNF-alpha, human, recombinant (E. coli) 106 

C-64420 VEGF-165, human, recombinant (E. coli) 106 

C-64423 VEGF-165, human, recombinant (HEK293) 106 

C-65112 TPO, human, recombinant (E. coli) 106 

C-67110 flt-3 Ligand, human, recombinant (E. coli) 106 

C-41210 DetachKit-125 101 

C-41212 DetachKit 2-125 101 

C-41220 DetachKit-250 101

130 



C-70000 – C-74999 

C-71110 DMEM, w/o L-Glutamine 90 

C-71111 DMEM, with L-Glutamine 90 

C-71115 DMEM, with 25 mM HEPES, 


C-71116 DMEM, with 25 mM HEPES, 


C-71120 DMEM, with stable Glutamine 90 

C-71210 DMEM, High Glucose (4.5g/l), 





with stable Glutamine 


with stable Glutamine, with Na-Pyruvate 


with L-Glutamine, with Na-Pyruvate 


w/o L-Glutamine, with Na-Pyruvate 

C-71310 DMEM/Ham´s F-12, w/o L-Glutamine 90 

C-71311 DMEM/Ham´s F-12, with L-Glutamine 90 

C-71320 DMEM/Ham´s F-12, with stable Glutamine 90 

C-72010 Ham´s F-10, w/o L-Glutamine 90 

C-72020 Ham´s F-10, with L-Glutamine 90 

C-72110 Ham´s F-12, w/o L-Glutamine 90 

C-72120 Ham´s F-12, with L-Glutamine 90 

C-73110 Leibovitz L-15, w/o L-Glutamine 90 

C-73210 McCoy's 5A, w/o L-Glutamine 91 

C-73211 McCoy’s 5A, with L-Glutamine 91 

C-73220 McCoy´s 5A, with stable Glutamine 91 

C-73227 McCoy´s 5A, with stable Glutamine, 


C-75000 – C-79999 

90 

90 

90 

90 

90 

90 

90 

90 

91 

PK 

PK-CA91-1024 PCR Mycoplasma Test Kit I/C 110 



PK-CA91-2024 PCR Bacteria Test Kit 112 

PK-CA91-2048 PCR Bacteria Test Kit 112 

PK-CA91-3025A PCR Mycoplasma Test Kit I/RT; Variant A 110 

PK-CA91-3025B PCR Mycoplasma Test Kit I/RT; Variant B 110 

PK-CA92-100 PCR Mycoplasma Test Kit III 110 

PK-CC01-867-1B Aquaguard-1 (incubator) 113 

PK-CC01-867-10B Aquaguard-1 (incubator) 113 

PK-CC01-916-1E Aquaguard-2 (water bath) 113 

PK-CC01-916-10E Aquaguard-2 (water bath) 113 

PK-CC03-036-1B BIOMYC-1 111 

PK-CC03-036-1C BIOMYC-1 111 

PK-CC03-036-1D BIOMYC-1 111 

PK-CC03-037-1B BIOMYC-2 111 

PK-CC03-037-1C BIOMYC-2 111 

PK-CC03-037-1D BIOMYC-2 111 

PK-CC03-038-1B BIOMYC-3 111 

PK-CC03-038-1C BIOMYC-3 111 

PK-CC03-038-1D BIOMYC-3 111 

PK-CC-110100 Pharmacidal Spray 113 

PK-CC-110100-5L Pharmacidal Spray 113 

PK-CC91-4003 Mycoplasma-EX 111 

PK-CC91-5050 Mycoplasma-EX Spray 113 

PK-CC91-5050-5L Mycoplasma-EX Spray 113 

PK-CC91-5060 Mycoplasma Disinfection Kit I 113 

PK-CC91-5070 Mycoplasma Disinfection Kit II 113 

PK-MB713-001N RTU DNA Ladder I (100 bp) 110 

PK-MB713-002N RTU DNA Ladder II (1000 bp) 110 

C-75012 MEM Alpha, w/o Nucleosides, 


C-75013 MEM Alpha, w/o Nucleosides, 


C-75014 MEM Alpha, with Nucleosides, 


91 

91 

91 

C-75100 MEM D-Valine, w/o L-Glutamine 91 

C-75210 MEM Earl, w/o L-Glutamine 91 

C-75211 MEM Earl, with L-Glutamine 91 

C-75220 MEM Earl, with stable Glutamine 91 

C-76010 RPMI 1640, w/o L-Glutamine 91 

C-76011 RPMI 1640, with L-Glutamine 91 

C-76015 RPMI 1640, with 25mM HEPES, 


C-76016 RPMI 1640, with 25mM HEPES, 


C-76017 RPMI 1640, w/o L-Glutamine, 


91 

91 

91 

C-76020 RPMI 1640, with stable Glutamine 91 

C-77110 Mitsuhashi/Maramorosh Insect Medium 92 

C-77210 Schneider's Drosophila Medium, 


92

International Distributors 131 

International Distributors 

Cat. AlbaniaNumber Product Biomedica Page Shpk 

Cat. Number Product Phone: +355-69 40 91 613 Page 

Rr Themistokli Germenji 

Fax: +355-45 00 681 

Kulla Ambasador, Kati 3, Ap 1 

Email: a.selimi@bmgrp.al 

1000 Tirana 

Internet: www.biomedica.co.at 

Albania 

Australia 

Banksia Scientific Company 

3/160 Lytton Road 

PO Box 529 

Bulimba QLD 4171 

Australia 

Phone: +61-7-39 02 30 00 

Fax: +61-7-32 17 98 69 

Free Call: 1300 769944 

Email: sales@banksiascientific.com.au 

Internet: www.banksiascientific.com.au 

Life Research Pty Ltd. 

Level 1, 5 Lakeside Drive 

Burwood East Victoria 3151 

Australia 

Phone: +61-3-98 03 00 45 

Fax: +61-3-86 48 58 41 

Email: info@liferesearch.com 

Internet: www.liferesearch.com 

Austria 

Biomedica Medizinprodukte GmbH & Co KG 

Divischgasse 4 

1210 Wien 

Austria 

Phone: +43-1-29 10 75 6 

Fax: +43-1-29 01 42 9 

Email: sales.biomedica@bmgrp.at 


Bangladesh 

KRISHGEN BioSystems 

Unit Nos#318/319 

3rd Floor 

Shah & Nahar Ind. Premises 

Dr E Moses Road 

Worli 

Mumbai 400018 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 

Email: sales@krishgen.com 

Internet: www.krishgen.com 

Bosnia-Herzegovina 

Biomedica d.o.o. 

Zmaja od Bosne 4 

71000 Sarajevo 

Bosnia 

Phone: +387-66 90 60 86 

Fax: +387-33 26 27 26 

Email: a.hodzic@bmgrp.ba 


Bulgaria 

Sofbiolife-Biomedica Ltd. 

52, Gen. Gurko Str. Floor 5 

1000 Sofia 

Bulgaria 

Phone: +359-2-98 89 151 

Fax: +359-2-98 11 883 

Email: office@biomedica-bg.eu 

Internet: www.biomedica-bg.eu 

Canada 

CEDARLANE 

4410 Paletta Court 

Burlington, Ontario, Canada 

L7L 5R2 

Canada 

Phone: 1-800-268-5058 (toll-free) 

Fax: +1 289-288-0020 

Email: general@cedarlanelabs.com 

Internet: www.cedarlanelabs.com 

MJS BioLynx Inc. 

300 Laurier Blvd. 

Brockville, Ontario, Canada 

K6V 5W1 

Canada 

Phone: 1-888-593-5969 (toll-free) 

Fax: +1 613 342-1341 

Email: sales@biolynx.ca 

Internet: www.biolynx.ca 

China 

Changzhou Hongyu Biomed Ltd. 

Tian Yuan Massion, Chang Wu zhong Road, 

Changzhou High Tech and Education Park 

Changzhou, Jiangsu 

China 

Phone: +86 519 89 85 93 22 

Fax: +86 519 89 85 93 23 

Email: info@promocell-china.com 

Internet: www.promocell-china.com 

Croatia 

Biomedica Dijagnostika d.o.o. 

V Ravnice 10 

10000 Zagreb 

Croatia 

Phone: +385 1 888 5727 

Fax: +385 1 888 5728 

Email: biomedica-dijagnostika@bmgrp.hr 


Czech Republic 

BIOMEDICA CS, s.r.o. 

Meteor Centre Office Park “B” 

Sokolovská 100/94 

186 00 Praha 8 

Czech Republic 

Phone: +420 2 8393 14 85 

Phone: +420 2 8393 36 05 

Fax: +420 2 8393 25 07 

Email: info@biomedica.cz 

Internet: www.biomedica.cz 

Denmark 

PromoCell GmbH 

Sickingenstr. 63/65 

69126 Heidelberg 

Germany 

Phone: +49 6221 - 649 34 0 

Fax: +49 6221 - 649 34 40 

Email: diana.hops@promocell.com 

Internet: www.promocell.com 

Finland 

Biotop Oy 

Tykistökatu 6B 3 

20520 Turku 

Finland 

Phone: +358-2-241 00 99 

Fax: +358-2-241 01 11 

Email: myynti@biotop.fi 

Internet: www.biotop.fi

132 

International Distributors 

Hungary 

Biomedica Hungária Kft. 

Ganz u. 16 

1027 Budapest 

Hungary 

Phone: +361-22 53 85 0 

Fax: +361-20 12 68 4 

Email: biomed@biomedica.hu 

Internet: www.biomedica.hu 

India 



3rd Floor 



Worli 

Mumbai 400018 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



Indonesia 



3rd Floor 



Worli 

Mumbai 400018 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



Israel 

Biological Industries Ltd. 

Kibbutz Beit Haemek, 25115 

Israel 

Phone: +972-4-996-0595 

Fax: +972-4-996-8896 

Email: info@bioind.com 

Internet: www.bioind.com 

Italy 

VWR International PBI S.r.l. 

Via San Giusto, 85 

20153 Milano 

Italy 

Phone: +39-02-48 77 91 

Fax: +39-02-40 09 00 10 

Email: info@it.vwr.com 

Internet: it.vwr.com 

Japan 

Funakoshi Co. Ltd. 

9-7 Hongo 2-chome 

Bunkyo-ku 

Tokyo 

Japan 113-0033 

Phone: +81-3-56 84 16 20 

Fax: +81-3-56 84 17 75 

Email: reagent@funakoshi.co.jp 

Internet: www.funakoshi.co.jp 

TaKaRa Bio Inc. 

Seta 3-4-1 

Otsu, Shiga 520-2193 

Japan 

Phone: +81-77-543 7247 

Fax: +81-77-543 9254 

Internet: www.takara-bio.co.jp 

Korea 

Sungwoo Life Science Co., Ltd. 

Samsung BLDG 4th Floor 560-5 

Uijeongbu 2Dong Uijeongbu-City 

Kyeonggi-Do 

South Korea 

Phone: +82-2-985-7471 

Fax: +82-31-876-9149 

Email: sungwoo@sungwools.com 

Internet: www.sungwools.com 

Macedonia 

Biomedika Dijagnostika dooel 

Ul. Dame Gruev bb, Zgrada Pelagonka, Kat 2 - Lokal 9 

1000 Skopje 

Macedonia 

Phone: +389 703 62 676 

Fax: +389 232 22 404 

Email: v.peeva@bmgrp.mk 


Malaysia 

Biomarketing Services SDN BHD 

21, Jalan 4/62A 

Bandar Menjalara, Kepong 

52200 Kuala Lumpur 

Malaysia 

Phone: +603 6273 3068 

Fax: +603 6272 0093 

Email: info@biomarketing.com.my 

Internet: www.biomarketing.com.my 

Nepal 



3rd Floor 



Worli 

Mumbai 400018 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



New Zealand 

Banksia Scientific Company 

12 Brigade Street 

Airport Oaks 

Auckland 

New Zealand 

Phone: +64-9-921 26 26 

Fax: +64-9-921 26 27 

Email: sales@banksiascientific.co.nz 

Internet: www.banksiascientific.co.nz 

Life Research Pty Ltd. 

Level 1, 5 Lakeside Drive 

Burwood East Victoria 3151 

Australia 

Phone: +61-3-98 03 00 45 

Fax: +61-3-86 48 58 41 

Email: info@liferesearch.com 

Internet: www.liferesearch.com 

Norway 




Germany 

Phone: +49 6221 - 649 34 0 

Fax: +49 6221 - 649 34 40 

Email: diana.hops@promocell.com 

Internet: www.promocell.com

International Distributors 133 

Poland 

Biomedica Poland sp. z.o.o. 

Ul. Raszyńska 13 

05-500 Piaseczno 

Poland 

Phone: +48-22 73 75 996 

Fax: +48-22 73 75 994 

Email: biomedica@bmgrp.pl 

Internet: www.biomedica.pl 

Portugal 

Labclinics, S.A. 

c/Industria 54 

08025 Barcelona 

Spain 

Phone: +34-93-446 47 00 

Fax: +34-93-348 10 39 

Email: info@labclinics.com 

Internet: www.labclinics.com 

Romania 

CYBER SRL Romania 

Str. Smaranda Braescu, nr. 20D 

Sector 1, Cod 014203 

70941 Bucharest 

Romania 

Phone: +40-37 27 66 844 

Fax: +40-21 23 22 204 

Email: dan.pavel@cybermed.ro 

Internet: www.cyber-srl.ro 

Serbia 

BIOMEDICA MP d.o.o. 

Cara Dusana 214 

11080 Zemun 

Serbia 

Phone: +381 11 6301 882 

Fax: +381 11 6301 883 

Email: office@biomedicamp.com 


Singapore 

Biomed Diagnostics Pte. Ltd. 

18 Boon Lay Way 

#05-105-108 TradeHub 21 

Singapore 609966 

Phone: +65-6-298 43 47 

Fax: +65-6-298 47 23 

Email: sales@biomed.com.sg 

Internet: www.biomed.com.sg 

Singlab Technologies Pte. Ltd. 

1003 Bukit Merah Central #07-50 

INNO ° CENTRE 

Singapore 159836 

Phone: +65-6-272 07 88 

Fax: +65-6-270 71 28 

Email: PromoCell@singlab.com.sg 

Internet: www.singlab.com.sg 

Slovakia 

Biomedica Slovakia s.r.o. 

Drobného 27 

84102 Bratislava 

Slovakia 

Phone: +421-2-69 30 99 01 

Fax: +421-2-69 30 99 08 

Email: biomedica@biomedica.sk 

Internet: www.biomedica.sk 

Slovenia 

Biomedis M.B. 

Slokanova ulica 12 

2000 Maribor 

Slovenia 

Phone: +386-2-47 16 300 

Fax: +386-2-47 16 304 

Email: info@biomedis-mb.si 

Internet: www.biomedis-mb.si 

South Korea 

Sungwoo Life Science Co., Ltd. 

Samsung BLDG 4th Floor 560-5 

Uijeongbu 2Dong Uijeongbu-City 

Kyeonggi-Do 

South Korea 

Phone: +82-2-985-7471 

Fax: +82-31-876-9149 

Email: sungwoo@sungwools.com 

Internet: www.sungwools.com 

Spain 

Labclinics, S.A. 

c/Industria 54 

08025 Barcelona 

Spain 

Phone: +34-93-446 47 00 

Fax: +34-93-348 10 39 

Email: info@labclinics.com 

Internet: www.labclinics.com 

Sri Lanka 



3rd Floor 



Worli 

Mumbai 400018 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



Sweden 

Mediqip AB 

Förrådsvägen 10 

141 46 Huddinge 

Sweden 

Phone: +46-8-448 10 10 

Fax: +46-8-448 10 75 

Email: info@mediqip.se 

Internet: www.mediqip.se 

Taiwan 

Biochiefdom International Co. Ltd. 

1F, No 50, Yishou St. 

Wenshan District 

Taipei 11659 

Taiwan 

Phone: +886-2-29 37 00 88 

Fax: +886-2-29 37 97 17 

Email: info@bio-chief.com.tw 

Internet: www.bio-chief.com.tw 

Thailand 

Biomed Diagnostics (Thailand) Co. Ltd. 

96/38 Moo 6 Bangkruai-Sainoi Rd 

Bangkruai 

Nontaburi, 11130 

Thailand 

Phone: +66-2-879 60 26 

Fax: +66-2-879 60 65 

Email: nan@biomedthai.com 

Internet: www.biomedthai.com

134 

Terms and Conditions 

Terms and Conditions 

General Terms 

1. These conditions of sale and delivery 

apply to all contracts of supply between 

us and our customers, even when in individual 

cases they are not referred to. 

2. A customer’s inconsistent conditions 

of purchase shall have no validity, even 

when we do not expressly reject such 

conditions. 

3. The customer is deemed to agree to 

these conditions of sale and delivery 

upon unconditional acceptance of the 

goods supplied. 

4. Rights of the customer arising during 

the course of business with us are not 

transferable. 

5. Any agreement that differs from these 

conditions of sale and delivery, or any 

recognition of the customer’s contradictory 

purchase conditions, requires our 

express confirmation in writing. 

Offers 

1. In the absence of a declaration to the 

contrary, we are not bound by any offers 

we make. 

2. Supply contracts only take effect when 

confirmed by us in writing. 

3. Any inquiries or complaints concerning 

confirmation are to be made promptly in 

writing. 

4. Assurances and assured properties 

only exist when they are expressly described 

as such. The descriptions and 

other statements in our catalogues and 

price lists are accurate to the best of our 

knowledge and belief. Under no circumstances 

may any assurances as to properties 

be derived from such descriptions 

and statements. 

Prices 

1. Our prices quoted in the current price 

list are net prices, VAT (value added tax) 

not included. 

2. VAT is charged according to the applicable 

laws in effect. 

3. Charges for delivery, cooling and insurance 

are indicated in the valid price 

list. 

4. PromoCell prices are subject to change 

without prior notice. 

Delivery 

1. Delivery times will be specified after 

careful co-ordination. They are however 

not legally binding. Claims for compensation 

due to delayed or non-delivery are 

excluded. 

2. If a delivery date specified by us is 

exceeded by more than two weeks, the 

customer is entitled to give us a further 

two week time limit for delivery. Should 

the delivery not be carried out by the 

end of the additional two week delivery 

period, the customer is entitled to rescind 

the contract. The rescission must 

be made promptly and in writing, at the 

latest within a week of the expiry of the 

additional two week delivery period. 

3. All deliveries take place at the customer’s 

risk. The passing of risk takes place 

on the handing over of the goods to the 

carrier or haulier. The careful choice of 

dispatch method and means of transport 

is at our discretion with exclusion of any 

and all liability. 

4. Deliveries in part are permitted. They 

are to be regarded as separate deliveries. 

Payment 

1. Payment is to be made without any 

deductions within 14 days of delivery 

and remittance of the bill. 

2. Default of payment will result in legal 

proceedings upon the expiry of the payment 

period without further warning. In 

this case, or in the case of a warning, we 

are entitled to interest for late payment 

at the current normal bank borrowing 

rate, or alternatively at a rate of 5% 

above the current discount rate of the 

Deutsche Bundesbank. 

3. A cash discount is only permitted 

when expressly stated on the bill and 

when payment is made within the stated 

period. 

4. An offset against or demand for 

payment is only permitted when the 

customer has an undisputed or legally 

established counterclaim. 

5. If our conditions of payment are not 

complied with or if justifiable doubts 

exist regarding the customer’s ability or 

willingness to pay, we are entitled to 

rescind the contract and to demand the 

return of the delivered goods, the costs 

of delivery to be borne by the customer. 

In addition, all time outstanding claims 

will become due. 

Justifiable doubts referred to above 

include, but are not restricted to: unfavourable 

information, deterioration of 

financial circumstances, initiation of insolvency 

or bankruptcy proceedings, execution 

measures against the customer, 

protest of bills, and default of payment 

for any other reason. 

6. If the customer does not take delivery 

of properly delivered goods, we are 

entitled, after setting an additional time 

limit, to rescind the contract, or to damages 

for non-compliance with the contract. 

In the latter case we are entitled to 

demand either damages of 25% of the 

sales price without proof, or the actual 

amount of damages. 

Complaint 

1. Any complaint regarding the goods, 

such as defects, lack of assured properties, 

wrong delivery and over- or underdelivery 

are, after immediate discontinuation 

of use, to be reported in writing. 

2. In the case of wrong delivery, the 

goods will be taken back at our expense 

and replaced with the goods ordered. 

Excess deliveries may similarly be sent 

back to us at our expense. In the case of 

under delivery, the customer may choose 

between a reduction in payment or complete 

fulfilment of the order. 

3. In the case of delivery of faulty goods 

we are entitled to replace the faulty 

goods with goods free of faults. Should 

the replacement delivery also be faulty, 

the customer may choose between a 

reduction in payment or cancellation of 

the contract. Claims for compensation 

are excluded except in the case of lack of 

assured properties. 

4. The right to complain expires in the 

case of obvious or apparent defects two 

weeks after receipt of the goods, and 

in the case of non-apparent defects six 

months after receipt of the goods. 

5. Goods may only be returned by arrangement 

with us and with the issue 

of a return number. The goods must be

Terms and Conditions 135 

properly packed. If returned goods are 

damaged due to the fault of the customer, 

the customer is obliged to pay 

compensation for such damage. 

6. In the case of delivery to traders the 

customer is to inspect the goods without 

delay upon receipt and to report 

any complaints to us immediately. If the 

customer fails to make such a report 

the goods will be deemed approved. In 

the case of a fault that is not apparent 

upon inspection, the complaint must be 

reported immediately upon discovery of 

the fault, otherwise the goods will be 

deemed approved as regards such fault. 

Use of Goods 

1. Goods delivered by us are intended 

exclusively for the purposes of research 

and may not be used on human beings, 

animals, in the household, or for any 

private use. We emphasise that the use 

of our products for diagnostic or therapeutic 

purposes is subject to current legal 

regulations. 

2. We do not accept any liability for the 

improper use of our products. We deliver 

only to commercial enterprises, resalers 

and public research, development and 

teaching establishments. Legal regulations 

require us to carefully check orders 

and to refuse delivery when there are 

signs of improper use of our products. 

Orders from students or other private 

individuals must be given from an official 

office of their institute or firm. 

3. We expressly oppose research or any 

uses which run counter to ethical, legal 

or political standards or principles within 

Germany and/or the import country, or 

whose ethical justification is the subject 

of public debate and dispute in the respective 

country (e.g. certain cloning 

experiments). 

We do not accept any liability for such 

research or use by our customers, or 

by third parties who have received our 

products from our customers. 

Liability 

1. All products delivered by us are only 

to be handled by trained personnel who 

are well aware of any potential dangers. 

Should any liability for compensation 

arise as a result of damage caused by 

breach of this condition, the customer 

shall be liable to pay any such compensation. 

In all cases, current safety regulations 

are to be observed, especially in the 

case of cells of human or animal origin, 

which always represent a potential risk 

of infection. The lack of a danger symbol 

does not mean that the substances concerned 

are harmless. 

2. We accept no responsibility for damage, 

loss, or injury caused by incorrect 

handling or use in the household, on 

humans, or on animals. In as far as we 

have made confirmation of delivery dependent 

upon a specified use of certain 

products, the customer accepts full liability 

for any damage suffered by us as 

a result of the customer’s incorrect use 

of the product. In the case of products 

which may only be used in accordance 

with legal or official regulations, the customer’s 

order simultaneously counts as 

a declaration that the products will be 

used for a specific purpose in the above 

sense. 

3. Our customers are required without 

exception to apply the laboratory guidelines 

of the professional association of 

the chemical industry when dealing with 

our products. 

4. Passing on of our products to private 

individuals is not permitted. If as a result 

of a breach of this condition for whatever 

reason we should receive a claim for 

compensation from a private individual, 

we shall have a right of redress against 

the customer. 

Indemnification 

1. In addition to section “complaint”, 

claims by the customer are excluded, in 

so far as they are not based upon intention 

or gross negligence. 

2. In the case of delivery to a trader this 

exclusion also applies to any damage 

caused by the intention or gross negligence 

of our business associates, carriers 

or employees. We accept no liability 

for damage caused by the behaviour of 

companies or individuals which sell our 

products. 

3. The sale of our products under their 

trade names for further commercial use 

does not mean that these products and 

their trade names may be freely used 

in general circulation. Some products, 

their production and use or their trade 

names are patented or protected trade 

names. The further commercial use of 

such products and their trade names occurs 

as regards a third party under the 

exclusive responsibility and liability of 

the customer. 

Miscellaneous 

1. German law shall apply to all contracts 

entered into by us, including those for 

deliveries abroad. We expressly reject the 

application of the Vienna UN-agreement 

relating to international sales contracts 

of 11. 04. 80. 

2. In the case of contracts with traders 

who do not belong to the traders specified 

in §4 of the code of commercial law, 

the place for fulfilment of contracts and 

the court of jurisdiction shall be Heidelberg. 

3. If any of the above conditions is held 

to be invalid, the validity of any of the 

other conditions shall not be affected. 

4. Only the German version of this Terms 

of Delivery and Payment is valid. This 

translation shall only serve as assistance 

for our customers. You can get a German 

version on request. 




Germany 

Managing Director: Dirk Hüttner 

AG Mannheim HRB 335480

136 

Ordering and Technical Support 

Ordering and Technical Support 

Ordering Information 

To place an order please use any of the 

following convenient options: 

Phone Fax Online 

Our customer service representatives are 

ready to assist you with ordering Monday 

through Friday 

USA/Canada 

1 – 866 – 251 – 2860 (toll free) 

Germany 

0800 – 776 66 23 (toll free) 

France 

0800 90 93 32 (toll free) 

United Kingdom 

0800 – 96 03 33 (toll free) 

Switzerland 

+49 6221 – 649 34 0 

Netherlands 

+49 6221 – 649 34 0 

Other Countries 

+49 6221 – 649 34 0 

Please copy the fax order form on the next 

page and send it to the following fax numbers: 

USA/Canada 

1 – 866 – 827 – 9219 (toll free) 

Germany 

0800 – 100 83 06 (toll free) 

France 

0800 90 27 36 (toll free) 


0800 – 169 85 54 (toll free) 

Switzerland 

+49 6221 – 649 34 40 

Netherlands 

+49 6221 – 649 34 40 


+49 6221 – 649 34 40 

Online ordering is available 24 hours/ 

day, 7 days per week. Our online ordering 

system is secure, fast, and convenient. 

Check out www.promocell.com for more 

information. 

Email 

Please send an e-mail to 

order@promocell.com and include the 

following information: 

Catalog number, name, size and quantity 

Shipping and billing address 

Contact person`s name and telephone 

number 

PromoCell directly supplies to Belgium, 

Canada, France, Germany, Ireland, 

Liechtenstein, Luxembourg, Monaco, 

Netherlands, Switzerland, United Kingdom, 

and the United States. 

All deliveries are carried out using our 

recommended carrier, unless an alternative 

agreement has been arranged 

with the customer. Delivery time and 

shipment costs are extremely favorable. 

Our local sales representatives 

and our multilingual customer service 

are looking forward to serving you! 

For current information about PromoCell 

distributors see www.promocell.com or 

contact us via e-mail or phone. 

Technical Support 

Providing excellent technical support 

for our products is our top priority. Our 

technical service team relies on years of 

laboratory experience to assist you with 

product selection and advice, to maximize 

product performance. 

You may reach our technical support via 

telephone/fax (see numbers on the reverse 

side of the catalog) or by sending 

an e-mail to: tech.support@promocell.com 

Technical support is completed by our 

online Knowledge Base www.promocell. 

com/knowledge-base which provides 

reference literature, application notes, 

and a library of technical questions and 

answers related to our primary cells and 

media. 

Product Use 

Products are sold for research use only 

and are not intended for use in diagnostic 

procedures or for therapeutic and 

medical purposes. 

Trademarks Used in this Catalog 

The trademarks, logos, and service 

marks (collectively the “Trademarks”) 

displayed in this catalog, including but 

not limited to those contained in each 

of the list below are trademarks or registered 

trademarks of PromoCell GmbH 

in Europe, the U.S. and other countries. 

All other brands and product names are 

trademarks or registered trademarks 

of their respective companies. Nothing 

contained in this catalog should be 

construed as granting, by implication, 

estoppel, or otherwise, any license or 

right to use any trademarks displayed 

in the catalog without the permission 

of PromoCell GmbH or such third 

party that may own the trademarks 

displayed in the catalog. The misuse of 

the trademarks displayed in the catalog, 

or any other content in the catalog, is 

strictly prohibited. Please be advised that 

PromoCell GmbH will aggressively enforce 

its intellectual property rights to 

the fullest extent of the law, including 

the seeking of criminal prosecution. 

PromoCell (PromoCell GmbH) 

PromoFectin (PromoCell GmbH) 

PromoFluor (PromoCell GmbH) 

PromoKine (PromoCell GmbH) 

DetachKit (PromoCell GmbH) 

SupplementPack (PromoCell GmbH) 

SupplementMix (PromoCell GmbH) 

Cell Culture Assistant (PromoCell GmbH) 

ABI Prism ® 

Cell Lab Quanta SC ® 

Cepheid Smart Cycler ® 

LightCycler ® 

Mastercycler ® 

RNAlater ®

Fax Order Form 

Shipping Address 

Invoice Address (if different from shipping address) 

Mr. Ms. Dr. Prof. 

Mr. 

Ms. Dr. Prof. 

First Name 

First Name 

Last Name 

Last Name 

Company / University 

Company / University 

Department / Institute 

Department / Institute 

Street, No 

Street, No 

ZIP, City 

ZIP, City 

Country 

Country 

Phone 

E-mail 

Order Details 

Catalog Number Product Name Quantity 

Please write in capital letters 

Please send me a copy of the 

Please send me your newsletters 

PromoCell catalog 

PromoCell 

PromoKine catalog 

PromoKine 

PromoCell Academy catalog PromoCell Academy 

Date, Signature




Germany 

USA/Canada 

Phone: 1 – 866 – 251 – 2860 (toll free) 

Fax: 1 – 866 – 827 – 9219 (toll free) 

Deutschland 

Telefon: 0800 – 776 66 23 (gebührenfrei) 

Fax: 0800 – 100 83 06 (gebührenfrei) 

France 

Téléphone: 0800 90 93 32 (ligne verte) 

Téléfax: 0800 90 27 36 (ligne verte) 


Phone: 0800 – 96 03 33 (toll free) 

Fax: 0800 – 169 85 54 (toll free) 

Switzerland 

Phone: +49 6221 – 649 34 0 

Fax: +49 6221 – 649 34 40 

Netherlands 

Phone: +49 6221 – 649 34 0 

Fax: +49 6221 – 649 34 40 


Phone: +49 6221 – 649 34 0 

Fax: +49 6221 – 649 34 40 

Email: 

info@promocell.com 

Promoting sustainable 

forest management 

www.pefc.org 

www.promocell.com

Cell Culture

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