Cell Culture
Cell Culture
Cell Culture
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<strong>Cell</strong> <strong>Culture</strong><br />
2012
<strong>Cell</strong> <strong>Culture</strong><br />
www.promocell.com<br />
<strong>Cell</strong> Biology<br />
www.promokine.info<br />
Training Courses<br />
www.promocell-academy.com<br />
Please visit our websites regularly for upcoming new products and training courses
Overview 1<br />
Overview<br />
1<br />
Human Primary <strong>Cell</strong>s<br />
2<br />
Human Stem and Blood <strong>Cell</strong>s<br />
3<br />
<strong>Cell</strong> Model Systems<br />
4<br />
Media and Sera<br />
5<br />
<strong>Cell</strong> Pellets<br />
6<br />
Reagents and Supplements<br />
7<br />
Microbial Detection and Elimination<br />
8<br />
PromoKine <strong>Cell</strong> Biology Products
2<br />
Contents<br />
Contents<br />
About Promo<strong>Cell</strong><br />
Promo<strong>Cell</strong> Ethical Standards ......................................... 4<br />
Introduction. ..................................................... 5<br />
1<br />
Human Primary <strong>Cell</strong>s<br />
1.1 Cardiac Myocytes . ............................................ 9<br />
1.2 Chondrocytes ............................................... 10<br />
1.3 Endothelial <strong>Cell</strong>s (Large Vessels) ................................. 11<br />
1.4 Endothelial <strong>Cell</strong>s (Microvascular) new cell ............................. types<br />
15<br />
1.5 Epithelial <strong>Cell</strong>s ............................................... 21<br />
1.6 Fibroblasts. ................................................. 25<br />
1.7 Follicle Dermal Papilla <strong>Cell</strong>s .................................... 28<br />
1.8 Hepatocytes ................................................ 29<br />
1.9 Keratinocytes new cell ............................................ types<br />
30<br />
1.10 Melanocytes . ............................................... 31<br />
1.11 Osteoblasts ................................................ 32<br />
1.12 Preadipocytes . .............................................. 33<br />
1.13 Skeletal Muscle <strong>Cell</strong>s ......................................... 34<br />
1.14 Smooth Muscle <strong>Cell</strong>s ......................................... 35<br />
Overview Human Primary <strong>Cell</strong>s ...................................... 40<br />
Human Primary <strong>Cell</strong>s by Tissue Type .................................. 43<br />
2<br />
Human Stem and Blood <strong>Cell</strong>s<br />
2.1 Mesenchymal Stem <strong>Cell</strong>s ...................................... 47<br />
2.2 Pericytes ................................................... 49<br />
2.3 CD34 + Progenitor <strong>Cell</strong>s ........................................ 50<br />
2.4 CD133 + Progenitor <strong>Cell</strong>s ....................................... 51<br />
2.5 Monocytes new .............................................. cell types<br />
52<br />
2.6 Mononuclear <strong>Cell</strong>s new cell ........................................ types<br />
54<br />
Overview Human Stem and Blood <strong>Cell</strong>s. ............................... 56<br />
3<br />
4 new<br />
<strong>Cell</strong> Model Systems<br />
3.1 Angiogenesis Assays and Kits ................................... 59<br />
Media and Sera<br />
4.1 Media for Primary <strong>Cell</strong>s cell .................................... types<br />
67<br />
4.2 Media for Stem and Blood <strong>Cell</strong>s new cell .............................. types<br />
86<br />
4.3 Classical Media .............................................. 90<br />
4.4 Insect <strong>Cell</strong> <strong>Culture</strong> Media. ...................................... 92<br />
4.5 Sera. ...................................................... 92
Contents 3<br />
5<br />
6 new<br />
<strong>Cell</strong> Pellets<br />
5.1 <strong>Cell</strong> Pellets new cell .............................................. types<br />
97<br />
Reagents and Supplements<br />
6.1 Detachment Reagents cell .................................... types<br />
101<br />
6.2 Antibiotics and Antimycotics ................................... 103<br />
6.3 Buffers and Salt Solutions ..................................... 104<br />
6.4 Supplements new cell ........................................... types<br />
105<br />
6.5 Cytokines and Growth Factors ................................. 106<br />
7<br />
Microbial Detection and Elimination<br />
7.1 Mycoplasma Test Kits ........................................ 109<br />
7.2 Mycoplasma Elimination Solutions. .............................. 110<br />
7.3 Bacteria Test Kit. ............................................ 112<br />
7.4 Disinfectants ............................................... 113<br />
8<br />
PromoKine <strong>Cell</strong> Biology Products<br />
8.1 <strong>Cell</strong> Analysis ............................................... 117<br />
8.2 Apoptosis ................................................. 117<br />
8.3 Fluorescent Labeling ......................................... 117<br />
8.4 Gene Cloning, Expression, and Silencing .......................... 118<br />
8.5 <strong>Cell</strong> Transfection ............................................ 118<br />
8.6 Antibodies and ELISAs. ....................................... 118<br />
8.7 Cytokines and Growth Factors ................................. 119<br />
9<br />
Appendix<br />
Alphabetical Index .............................................. 120<br />
Numerical Index ................................................ 124<br />
International Distributors .......................................... 131<br />
Terms and Conditions ............................................ 134<br />
Ordering and Technical Support. .................................... 136<br />
Fax Order Form ................................................. 137
4 About Promo<strong>Cell</strong><br />
Promo<strong>Cell</strong> Ethical Standards<br />
Ethical and legal standards<br />
governing Primary Human <strong>Cell</strong>s<br />
Promo<strong>Cell</strong> is the original manufacturer<br />
of all primary, stem, and blood cells featured<br />
in this catalog and is committed to<br />
the highest ethical and legal standards.<br />
Consequently, Promo<strong>Cell</strong> strictly complies<br />
with the following procedure for<br />
the donation and collection of human<br />
tissue used for cell isolation:<br />
The tissue used by Promo<strong>Cell</strong> for the<br />
isolation of human cell cultures is derived<br />
from donors who have signed<br />
an informed consent form (this being<br />
done by the donor himself, an authorized<br />
agent, or a legal agent) which<br />
outlines in detail the purpose of the<br />
donation and the procedure for processing<br />
the tissue. The tissue used by<br />
Promo<strong>Cell</strong> is collected exclusively in<br />
Germany.<br />
Extract from “The Convention of Human Rights and Biomedicine”:<br />
When obtaining and using human<br />
tissue, Promo<strong>Cell</strong> acts in strict compliance<br />
with<br />
Article 5 – General rule<br />
An intervention in the health field may only be carried out after the person<br />
concerned has given free and informed consent to it.<br />
This person shall beforehand be given appropriate information as to the purpose<br />
and nature of the intervention as well as on its consequences and risks.<br />
The person concerned may freely withdraw consent at any time.<br />
Article 22 – Disposal of a removed part of the human body<br />
When in the course of an intervention any part of a human body is removed,<br />
it may be stored and used for a purpose other than that for which it was<br />
removed, only if this is done in conformity with appropriate information and<br />
consent procedures.<br />
Extract from “The Declaration of Helsinki”:<br />
Article 22<br />
In any research on human beings, each potential subject must be adequately<br />
informed of the aims, methods, sources of funding, any possible conflicts<br />
of interest, institutional affiliations of the researcher, the anticipated benefits<br />
and potential risks of the study and the discomfort it may entail. The subject<br />
should be informed of the right to abstain from participation in the study or<br />
to withdraw consent to participate at any time without reprisal. After ensuring<br />
that the subject has understood the information, the physician should then<br />
obtain the subject‘s freely-given informed consent, preferably in writing. If<br />
the consent cannot be obtained in writing, the non-written consent must be<br />
formally documented and witnessed.<br />
1. “The Convention for Protection<br />
of Human Rights and Dignity of the<br />
Human Being with Regard to the<br />
Application of Biology and Medicine:<br />
Convention of Human Rights and<br />
Biomedicine” published on April 4 th ,<br />
1997 by the Council of Europe (European<br />
Treaty Series – no 164).<br />
2. “The Human Tissue Act” published<br />
on November 15 th , 2004 by the<br />
government of the United Kingdom.<br />
This act aims to make consent a fundamental<br />
principle underpinning the<br />
use and storage of human tissue.<br />
3. “The Declaration of Helsinki” developed<br />
by the World Medical Association<br />
as a statement of ethical principles<br />
to provide guidance to physicians and<br />
other participants in medical research<br />
involving human subjects.<br />
Should you have any questions concerning<br />
ethical aspects related to the<br />
use of Primary Human <strong>Cell</strong>s, do not<br />
hesitate to contact us or visit our<br />
website:<br />
www.promocell.com/ethics
About Promo<strong>Cell</strong><br />
5<br />
The Promo<strong>Cell</strong> Customer Care Team<br />
Dear Customer,<br />
For more than 20 years Promo<strong>Cell</strong> has been your premier manufacturer<br />
of primary cell culture products, with a broad range of human primary<br />
cells, stem cells, and blood cells as well as optimized cell culture media.<br />
We are proud that scientists worldwide use Promo<strong>Cell</strong> products in basic<br />
and applied biomedical research to obtain better results from more accurate<br />
physiological models.<br />
With our new catalog we are happy to offer an extended stem and blood<br />
cell product range now covering Dendritic <strong>Cell</strong> Research with a comprehensive<br />
media portfolio and CD14 + monocytes for several applications.<br />
In addition, we have developed a xeno-free cell culture system for mesenchymal<br />
stem cells facilitating chemically defined culture conditions.<br />
In chapter 8 we would like to present the PromoKine brand, which offers<br />
a wide range of well proven products for cell biology research, such as<br />
cell analysis kits, cell transfection reagents, fluorescent dyes, antibodies,<br />
ELISAs and cytokines.<br />
As scientists, we understand the needs of fellow scientists, our customers.<br />
This feeling is the stimulus for how we act: Our mission, is to help<br />
you obtain optimal results in your daily labwork.<br />
Scientists supporting Scientists – guaranteed.<br />
Your Promo<strong>Cell</strong> Team
Human Primary <strong>Cell</strong>s<br />
1<br />
Experiments with human primary cells simulate in vivo conditions<br />
much better and yield more significant results than those<br />
using immortalized cell lines. This is because the primary cells’<br />
genome and expression patterns have not been altered by<br />
immortalization and numerous passages in culture.<br />
Promo<strong>Cell</strong> offers a wide variety of high quality human primary<br />
cells from tissue obtained from approved medical centers.
8<br />
Content Chapter 1<br />
1<br />
Human Primary <strong>Cell</strong>s<br />
1.1 Cardiac Myocytes .................... 9<br />
1.2 Chondrocytes ...................... 10<br />
1.3 Endothelial <strong>Cell</strong>s (Large Vessels) ........ 11<br />
Umbilical Vein ...................... 11<br />
Umbilical Artery. .................... 12<br />
Aortic / Coronary Artery .............. 13<br />
Pulmonary Artery / Saphenous Vein ..... 14<br />
1.4 Endothelial <strong>Cell</strong>s (Microvascular) ........ 15<br />
Dermal ........................... 15<br />
Dermal Blood ...................... 16<br />
Dermal Lymphatic ................... 17<br />
Bladder / Cardiac. ................... 18<br />
Pulmonary / Uterine ................. 19<br />
Brain new ........................... 20<br />
1.5 Epithelial <strong>Cell</strong>s ...................... 21<br />
Nasal / Tracheal. .................... 21<br />
Bronchial / Small Airway .............. 22<br />
Mammary / Renal ................... 23<br />
Renal Cortical / Placental. ............. 24<br />
1.6 Fibroblasts. ........................ 25<br />
Dermal ........................... 25<br />
Pulmonary / Aortic Adventitial / Cardiac .. 26<br />
Uterine / Villous Mesenchymal ......... 27<br />
1.7 Follicle Dermal Papilla <strong>Cell</strong>s ............ 28<br />
1.8 Hepatocytes ....................... 29<br />
1.9 Keratinocytes new .................... 30<br />
1.10 Melanocytes ....................... 31<br />
1.11 Osteoblasts ........................ 32<br />
1.12 Preadipocytes ...................... 33<br />
1.13 Skeletal Muscle <strong>Cell</strong>s ................. 34<br />
1.14 Smooth Muscle <strong>Cell</strong>s ................. 35<br />
Aortic ............................ 35<br />
Coronary Artery / Pulmonary Artery ..... 36<br />
Umbilical Artery / Tracheal ............ 37<br />
Bronchial / Bladder .................. 38<br />
Prostate / Uterine ................... 39<br />
Overview Human Primary <strong>Cell</strong>s .............. 40<br />
Human Primary <strong>Cell</strong>s by Tissue Type .......... 43
1 Human Primary <strong>Cell</strong>s 9<br />
Human Primary <strong>Cell</strong>s<br />
Promo<strong>Cell</strong> offers a wide variety of high<br />
quality human primary cells. The tissue<br />
used for the isolation of these cells is<br />
obtained from approved medical centers<br />
following strict ethical standards<br />
(see page 4 for details). Shortly after<br />
isolation, the cells are cryopreserved<br />
using the unique serum-free Promo<strong>Cell</strong><br />
Cryo-SFM (see page 85) and an optimized,<br />
computer controlled freezing<br />
profile. This allows high viability of the<br />
cells and defined culture conditions after<br />
thawing.<br />
All Promo<strong>Cell</strong> primary cells are available<br />
cryopreserved or proliferating. Each cell<br />
lot is tested for cell type specific markers,<br />
cell morphology, population doubling<br />
time, and proliferation capacity. In addition,<br />
tests for the absence of HIV 1, HIV 2,<br />
HBV, HCV, fungi, mycoplasma, and other<br />
bacteria are performed.<br />
Moreover, cell pellets prepared from released<br />
cell lots and stored in RNAlater ®<br />
are available. They are useful for DNA,<br />
RNA, and protein isolation and their subsequent<br />
analysis (for more information<br />
see page 97).<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
1.1 Cardiac Myocytes<br />
Applications<br />
Cardiac research<br />
Physiological studies<br />
Pharmacological studies<br />
Features<br />
Adult origin<br />
Sarcomeric α-actinin positive<br />
Slow muscle myosin positive<br />
Qualified for long-term experiments<br />
Human Cardiac Myocytes (HCM) are<br />
isolated from the ventricles of the adult<br />
heart. They are qualified for in vitro<br />
research on cardiac diseases and for<br />
pharmacological studies. Unlike freshly<br />
isolated rod-shaped myocytes, cultured<br />
HCM can be used for long-term experiments<br />
like investigating the long-term<br />
effects of cytokines, mechanical strain,<br />
or cell-cell interactions, as they are prepared<br />
according to a special protocol.<br />
Initially, the HCM act more like progenitor<br />
cells in that they are not yet fully differentiated.<br />
They express the markers<br />
of early stage differentiation such as<br />
GATA-4 and sarcomeric alpha-actin and<br />
have a high capacity for proliferation.<br />
When they are grown to confluency<br />
and cultivated for an extended period of<br />
time, the differentiation process begins.<br />
Markers of late differentiation (e.g. sarcomeric<br />
alpha-actinin, slow muscle myosin)<br />
are increased and the cells begin to<br />
form myotube-like structures.<br />
Recommended media & reagents<br />
Myocyte Growth Medium (see page 68)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Fig. 1: HCM culture in phase contrast.<br />
Related products<br />
Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 18)<br />
Human Cardiac Fibroblasts<br />
(see page 26)<br />
HCM Pellet (see page 97)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
Count<br />
75 Sarcomeric<br />
Sarcomeric<br />
α-actinin negative α-actinin positive<br />
Fig. 2: Flow cytometric analysis of Promo<strong>Cell</strong> HCM.<br />
96 % of the cells are sarcomeric α-actinin positive.<br />
50<br />
25<br />
0<br />
0<br />
10 10 2 10 3 10 4<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
Sarcomeric α-actinin<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Cardiac Myocytes (HCM)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12810<br />
C-12811<br />
HCM Pellet 1x10 6 cells in RNAlater ® C-14080
10<br />
1 Human Primary <strong>Cell</strong>s<br />
1.2 Chondrocytes<br />
Applications<br />
produce and maintain the extracellular<br />
Recommended media & reagents<br />
matrix of cartilage, i.e. collagen (mostly<br />
Chondrocyte Growth Medium<br />
Osteoarthritis research<br />
type II) and proteoglycans (primarily ag-<br />
(see page 68)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Joint research<br />
Physiology studies<br />
Chondrocyte differentiation<br />
experiments<br />
Hyaline cartilage investigations<br />
grecan).<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HCH Pellet (see page 97)<br />
PromoFectin Transfection Reagent<br />
Features<br />
(see PromoKine catalog)<br />
Available from hip and knee joints<br />
Differentiation tested by Alcian Blue<br />
staining of HCH spheroids<br />
Fig. 1: HCH culture in phase contrast.<br />
Cryopreserved at second passage<br />
Human Chondrocytes (HCH) are isolated<br />
from normal human articular cartilage<br />
from the knee and hip joints (lot specific<br />
source information is available on request).<br />
Promo<strong>Cell</strong> HCH are routinely<br />
tested for their capacity to differentiate<br />
in 3D spheroids (see Fig. 2).<br />
The articular cartilage covers the joints<br />
between bones and contains no blood<br />
vessels, lymphatic vessels, or nerve fi-<br />
Fig. 2: Spheroids of differentiated HCH<br />
bers. It is composed of only one special-<br />
cultured in Chondrocyte Growth Medium<br />
ized cell type, the chondrocytes, which<br />
and stained with Alcian Blue.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Chondrocytes (HCH)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12710<br />
C-12750<br />
HCH Pellet 1x10 6 cells in RNAlater ® C-14070
1 Human Primary <strong>Cell</strong>s 11<br />
1.3 Endothelial <strong>Cell</strong>s (Large Vessels)<br />
Applications<br />
Angiogenesis research<br />
Atherosclerosis research<br />
Oncology<br />
Drug uptake studies<br />
<strong>Cell</strong>-cell junction investigation<br />
Inflammation studies<br />
Features<br />
Tunica externa<br />
(Connective tissue)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Von Willebrand factor (vWF) positive<br />
CD31 positive<br />
Smooth muscle α-actin negative<br />
Tunica media<br />
(Smooth muscle cells)<br />
The endothelium is the thin layer of endothelial<br />
cells that line the interior surface<br />
of blood and lymphatic vessels - from the<br />
aorta to the smallest capillaries. In large<br />
vessels (arteries and veins), it is enclosed<br />
by two other layers namely the tunica media<br />
and the tunica externa (see Fig. 1).<br />
Promo<strong>Cell</strong> endothelial cells (large vessels)<br />
are available from the umbilical<br />
vein and artery, the aorta, the coronary<br />
artery, the pulmonary artery, and the<br />
saphenous vein. All endothelial cells are<br />
tested for positive expression of vWF and<br />
CD31, and the absence of smooth muscle<br />
α-actin.<br />
Available endothelial cell types (large vessels):<br />
HUVEC (see page 11)<br />
HUAEC (see page 12)<br />
HAoEC (see page 13)<br />
HCAEC (see page 13)<br />
HPAEC (see page 14)<br />
HSaVEC (see page 14)<br />
Tunica intima<br />
(Endothelial cells)<br />
Fig. 1: Cross section of an artery with the predominant cell types.<br />
Fig. 2: HUVEC culture stained for vWF Fig. 3: HUVEC culture stained for CD31<br />
(Factor VIII antigen). Nuclei counterstained<br />
with DAPI.<br />
antigen. Nuclei counterstained with<br />
DAPI.<br />
Human Umbilical Vein Endothelial<br />
<strong>Cell</strong>s (HUVEC)<br />
HUVEC are isolated from the vein of the<br />
umbilical cord and are commonly used<br />
for physiological and pharmacological<br />
investigations, such as macromolecule<br />
transport, blood coagulation, angiogenesis,<br />
and fibrinolysis.<br />
Promo<strong>Cell</strong> offers HUVEC from single<br />
donors or pooled from up to four<br />
different donors*.<br />
In addition, HUVEC are available which<br />
are pre-screened for angiogenesis and<br />
other VEGF (vascular endothelial growth<br />
factor) dependent signaling studies.<br />
They have been positively tested for<br />
VEGF response using Promo<strong>Cell</strong>´s unique<br />
3D-Angiogenesis Assay (see page 59).<br />
VEGF is an important signaling protein<br />
involved in both vasculogenesis<br />
and angiogenesis. In vitro, VEGF has<br />
been shown to stimulate endothelial<br />
cell mitogenesis, cell migration, sprouting,<br />
and microvascular permeability.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
(see page 69)<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
(see page 69)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HUVEC Pellet (see page 98)<br />
HUVEC pooled Pellet (see page 98)<br />
HUVEC pre-screened Pellet<br />
(see page 98)<br />
Human Umbilical Artery Endothelial<br />
<strong>Cell</strong>s (see page 12)<br />
Angiogenesis Assays and Kits<br />
(see page 59-63)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
VEGF (see page 106)<br />
CD31 and VEGF Antibodies<br />
(see www.promokine.info)<br />
* Human Umbilical Artery Endothelial <strong>Cell</strong>s (HUAEC) from the same donor are available on request.
12<br />
1 Human Primary <strong>Cell</strong>s<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
50 µm 50 µm<br />
Fig. 4: HUVEC culture in phase contrast.<br />
Fig. 5: Unstimulated (left) and VEGF (20 ng/ml) stimulated HUVEC-spheroids (right).<br />
See also 3D-Angiogenesis Assay, page 59.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Umbilical Vein Endothelial <strong>Cell</strong>s<br />
(HUVEC) single donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12200<br />
C-12250<br />
HUVEC Pellet 1x10 6 cells in RNAlater ® C-14010<br />
Human Umbilical Vein Endothelial <strong>Cell</strong>s<br />
(HUVEC) pooled<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12203<br />
C-12253<br />
HUVEC pooled Pellet 1x10 6 cells in RNAlater ® C-14011<br />
Human Umbilical Vein Endothelial <strong>Cell</strong>s<br />
(HUVEC) pre-screened<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12205<br />
C-12255<br />
HUVEC pre-screened Pellet 1x10 6 cells in RNAlater ® C-14012<br />
Human Umbilical Artery<br />
Endothelial <strong>Cell</strong>s (HUAEC)<br />
HUAEC are isolated from the arteries of<br />
the umbilical cord and are often used for<br />
in vitro studies on atherosclerosis but<br />
also for endothelial cell-dependent lymphocyte<br />
activation and chemoattractant<br />
activity experiments * .<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
(see page 69)<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
(see page 69)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HUAEC Pellet (see page 98)<br />
Human Umbilical Vein Endothelial<br />
<strong>Cell</strong>s (see page 11)<br />
Human Umbilical Artery Smooth<br />
Muscle <strong>Cell</strong>s (see page 37)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Umbilical Artery Endothelial <strong>Cell</strong>s<br />
(HUAEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12202<br />
C-12252<br />
HUAEC Pellet 1x10 6 cells in RNAlater ® C-14013<br />
*Human Umbilical Vein Endothelial <strong>Cell</strong>s (HUVEC) from the same donor are available on request.
1 Human Primary <strong>Cell</strong>s 13<br />
Human Aortic Endothelial <strong>Cell</strong>s<br />
(HAoEC)<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
Related products<br />
HAoEC Pellet (see page 97)<br />
(see page 70)<br />
Human Aortic Smooth Muscle <strong>Cell</strong>s<br />
HAoEC are isolated from the human<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 35)<br />
ascending (thoracic) and descending<br />
(abdominal) aorta. They are useful<br />
for studying vascular diseases such as<br />
thrombosis, atherosclerosis, and hypertension<br />
as well as for stent-graft compat-<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Human Aortic Adventitial Fibroblasts<br />
(see page 26)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
ibility testing.<br />
(see www.promokine.info)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Aortic Endothelial <strong>Cell</strong>s (HAoEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12271<br />
C-12272<br />
HAoEC Pellet 1x10 6 cells in RNAlater ® C-14023<br />
Human Coronary Artery<br />
Endothelial <strong>Cell</strong>s (HCAEC)<br />
HCAEC are isolated from the coronary<br />
arteries (the right and the left coronary<br />
artery, including the anterior descending<br />
and the circumflex branches) from a<br />
single donor. They are crucially involved<br />
in the regulation of coronary blood flow<br />
and cardiac functions and are consequently<br />
useful for in vitro studies of<br />
diseases such as thrombosis, atherosclerosis,<br />
and hypertension. HCAEC are also<br />
suitable for studying functional vasodilators,<br />
such as prostacyclin, bradykinin,<br />
or nitric oxide.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
(see page 70)<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Left main coronary<br />
artery<br />
Circumflex coronary<br />
artery<br />
Related products<br />
HCAEC Pellet (see page 97)<br />
Human Coronary Artery Smooth<br />
Muscle <strong>Cell</strong>s (see page 36)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
Right coronary artery<br />
Fig. 6: Location of the coronary arteries.<br />
Left anterior descending<br />
coronary artery<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Coronary Artery Endothelial <strong>Cell</strong>s<br />
(HCAEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12221<br />
C-12222<br />
HCAEC Pellet 1x10 6 cells in RNAlater ® C-14022
14<br />
1 Human Primary <strong>Cell</strong>s<br />
Human Pulmonary Artery<br />
Endothelial <strong>Cell</strong>s (HPAEC)<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
Related products<br />
HPAEC Pellet (see page 98)<br />
(see page 69)<br />
Human Pulmonary Artery Smooth<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
HPAEC are isolated from human pulmonary<br />
arteries (the main pulmonary<br />
artery, left and right branches). Since the<br />
pulmonary arteries carry blood from the<br />
heart to the lungs, they are the only ar-<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
(see page 69)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Muscle <strong>Cell</strong>s (see page 36)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
teries (other than umbilical arteries) that<br />
carry deoxygenated blood.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Pulmonary Artery Endothelial <strong>Cell</strong>s<br />
(HPAEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12241<br />
C-12242<br />
HPAEC Pellet 1x10 6 cells in RNAlater ® C-14024<br />
Human Saphenous Vein<br />
Endothelial <strong>Cell</strong>s (HSaVEC)<br />
HSaVEC are isolated from human saphenous<br />
veins from single donors. The<br />
saphenous vein is often used for autotransplantation<br />
in coronary artery bypass<br />
operations, when arterial grafts are not<br />
available or many grafts are required.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
(see page 69)<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
(see page 69)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HSaVEC Pellet (see page 98)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Saphenous Vein Endothelial <strong>Cell</strong>s<br />
(HSaVEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12231<br />
C-12232<br />
HSaVEC Pellet 1x10 6 cells in RNAlater ® C-14025
1 Human Primary <strong>Cell</strong>s 15<br />
1.4 Endothelial <strong>Cell</strong>s (Microvascular)<br />
Applications<br />
Angiogenesis research<br />
Oncology<br />
Drug uptake studies<br />
<strong>Cell</strong>-cell junction investigation<br />
Inflammation studies<br />
Features<br />
Von Willebrand factor (vWF) positive<br />
CD31 positive<br />
Smooth muscle α-actin negative<br />
Cryopreserved at second passage<br />
The walls of capillaries are composed of a<br />
single layer of microvascular endothelial<br />
cells. This layer is so thin that molecules<br />
such as oxygen, water, and lipids can pass<br />
through it by diffusion and enter the surrounding<br />
tissues. Waste products such<br />
as carbon dioxide and urea, on the other<br />
hand, diffuse back into the blood to be<br />
carried away. Because of their location<br />
at the interface between the circulating<br />
fluid in the lumen and the surrounding<br />
tissue, microvascular endothelial cells are<br />
highly active and closely involved in numerous<br />
physiological processes.<br />
Microvascular endothelial cells differ in<br />
morphologies and properties according<br />
to the tissues supplied by the capillaries.<br />
Therefore, Promo<strong>Cell</strong> microvascular endothelial<br />
cells are available from dermal,<br />
lung, cardiac, uterine, bladder, and brain<br />
tissues.<br />
Available endothelial cell types (microvascular):<br />
HDMEC (see page 15)<br />
HDBEC (see page 16)<br />
HDLEC (see page 17)<br />
HBdMEC (see page 18)<br />
HCMEC (see page 18)<br />
HPMEC (see page 19)<br />
HUtMEC (see page 19)<br />
HBMEC (see page 20)<br />
Fig. 1: HDMEC culture stained for vWF<br />
(Factor VIII antigen). Nuclei counterstained<br />
with DAPI.<br />
Fig. 2: HDMEC culture stained for CD31<br />
antigen. Nuclei counterstained with DAPI.<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC)<br />
HDMEC are isolated from the dermis of<br />
juvenile foreskin and adult skin (different<br />
locations). Since the dermis contains<br />
blood and lymphatic capillaries, HDMEC<br />
comprise Blood and Lymphatic Microvascular<br />
Endothelial <strong>Cell</strong>s. Both have a<br />
common origin and can be identified by<br />
several markers.<br />
Beside standard HDMEC, pre-screened<br />
HDMEC are also available. They have<br />
been positively tested for VEGF response<br />
using Promo<strong>Cell</strong>´s unique 3D-Angiogenesis<br />
Assay (see page 59). VEGF is an<br />
important signaling protein involved in<br />
both vasculogenesis and angiogenesis.<br />
In vitro, VEGF has been shown to stimulate<br />
endothelial cell mitogenesis, cell<br />
migration, sprouting, and microvascular<br />
permeability.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
(see page 70)<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HDMEC Pellet (see page 97)<br />
HDMEC adult Pellet (see page 97)<br />
HDMEC pre-screened Pellet<br />
(see page 97)<br />
Human Pericytes (see page 49)<br />
Angiogenesis Assays and Kits<br />
(see page 59-63)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
VEGF (see page 106)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
Fig. 3: HDMEC culture in phase contrast.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Dermal Microvascular Endothelial<br />
<strong>Cell</strong>s (HDMEC) juvenile foreskin<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12210<br />
C-12260<br />
HDMEC Pellet 1x10 6 cells in RNAlater ® C-14015<br />
Human Dermal Microvascular Endothelial<br />
<strong>Cell</strong>s (HDMEC) adult donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12212<br />
C-12262<br />
HDMEC adult Pellet 1x10 6 cells in RNAlater ® C-14016<br />
Human Dermal Microvascular Endothelial<br />
<strong>Cell</strong>s (HDMEC) pre-screened<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12215<br />
C-12265<br />
HDMEC pre-screened Pellet 1x10 6 cells in RNAlater ® C-14017
16<br />
1 Human Primary <strong>Cell</strong>s<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human Dermal Blood Endothelial<br />
<strong>Cell</strong>s (HDBEC)<br />
HDBEC are isolated from the dermis of<br />
juvenile foreskin and adult skin (different<br />
locations) from a single donor*.<br />
The cells are routinely analyzed by flow<br />
cytometric analysis and immunofluorescent<br />
staining: they are > 95% CD31<br />
positive (see Fig. 4) and stain positive for<br />
von Willebrand factor. In addition, they<br />
are > 90% negative for podoplanin<br />
(see Fig. 5) and stain negative for<br />
smooth muscle specific α-actin.<br />
Blood Endothelial <strong>Cell</strong>s have a key function<br />
in physiological processes like vessel<br />
tonus, capillary permeability, blood coagulation,<br />
fibrolysis, and angiogenesis.<br />
They are also useful for disease studies<br />
of atherosclerosis, tumorigenesis, and<br />
thrombosis.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HDBEC Pellet (see page 97)<br />
HDBEC adult Pellet (see page 97)<br />
Human Dermal Lymphatic Endothelial<br />
<strong>Cell</strong>s (see page 17)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
Podoplanin, CD31, and Prox-1<br />
Antibodies (see www.promokine.info)<br />
Count<br />
75<br />
CD31 negative<br />
CD31 positive<br />
Fig. 4: Flow cytometric analysis of Promo<strong>Cell</strong> HDBEC.<br />
98% of the cells are CD31 positive .<br />
50<br />
25<br />
0<br />
0<br />
10 10 2 10 3 10 4<br />
CD31<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
Count<br />
75<br />
Podoplanin negative<br />
Podoplanin positive<br />
Fig. 5: Flow cytometric analysis of Promo<strong>Cell</strong> HDBEC.<br />
91% of the cells are podoplanin negative.<br />
50<br />
25<br />
0<br />
0<br />
10 10 2 10 3 10 4<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
Podoplanin<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Dermal Blood Endothelial <strong>Cell</strong>s<br />
(HDBEC) juvenile foreskin<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12211<br />
C-12214<br />
HDBEC Pellet 1x10 6 cells in RNAlater ® C-14018<br />
Human Dermal Blood Endothelial <strong>Cell</strong>s<br />
(HDBEC) adult donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12225<br />
C-12226<br />
HDBEC adult Pellet 1x10 6 cells in RNAlater ® C-14019<br />
*Human Dermal Lymphatic Microvascular Endothelial <strong>Cell</strong>s (HDLEC) from the same donor are available on request.
1 Human Primary <strong>Cell</strong>s 17<br />
Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s (HDLEC)<br />
HDLEC are isolated from the dermis of<br />
juvenile foreskin and adult skin (different<br />
locations) from a single donor*. The cells<br />
are routinely analyzed by flow cytometric<br />
analysis and immunofluorescent staining:<br />
> 95% of the cells are CD31 positive and<br />
podoplanin positive (see Fig. 6 and 7).<br />
In addition, the cells stain positive for<br />
Prox-1.<br />
Lymphatic vessels transport excess fluids<br />
from tissues to the circulatory system and<br />
are a major component of the immune<br />
system. The transported fluid is nearly<br />
cell free. The vessels are highly permeable,<br />
because they lack a continuous<br />
basal membrane. Lymphatic endothelial<br />
cells are involved in pathological alterations<br />
of the lymphatic system. During<br />
tumor lymphangiogenesis, the lymphatic<br />
endothelial cells build new vessels that<br />
infiltrate tumors, attract tumor cells, and<br />
induce tumor cell metastasis.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HDLEC Pellet (see page 97)<br />
HDLEC adult Pellet (see page 97)<br />
Human Dermal Blood Endothelial<br />
<strong>Cell</strong>s (see page 16)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
VEGF (see page 106)<br />
Podoplanin, CD31, and Prox-1<br />
Antibodies (see www.promokine.info)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Count<br />
75<br />
CD31 negative<br />
CD31 positive<br />
Fig. 6: Flow cytometric analysis of Promo<strong>Cell</strong> HDLEC.<br />
99% of the cells are CD31 positive.<br />
50<br />
25<br />
0<br />
0 10 10 2 10 3 10 4<br />
CD31<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
Fig. 7: Flow cytometric analysis of Promo<strong>Cell</strong> HDLEC.<br />
96% of the cells are podoplanin positive.<br />
Count<br />
75<br />
Podoplanin negative Podoplanin positive<br />
50<br />
25<br />
0<br />
0 10 10 2 10 3 10 4<br />
Podoplanin<br />
<strong>Cell</strong> Lab Quanta SC T M , Beckman Coulter, Inc.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Dermal Lymphatic Endothelial<br />
<strong>Cell</strong>s (HDLEC) juvenile foreskin<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12216<br />
C-12218<br />
HDLEC Pellet 1x10 6 cells in RNAlater ® C-14020<br />
Human Dermal Lymphatic Endothelial<br />
<strong>Cell</strong>s (HDLEC) adult donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12217<br />
C-12219<br />
HDLEC adult Pellet 1x10 6 cells in RNAlater ® C-14021<br />
*Human Dermal Blood Endothelial <strong>Cell</strong>s (HDBEC) from the same donor are available on request.
18<br />
1 Human Primary <strong>Cell</strong>s<br />
Human Bladder Microvascular<br />
Endothelial <strong>Cell</strong>s (HBdMEC)<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
Related products<br />
HBdMEC Pellet (see page 97)<br />
(see page 70)<br />
PromoFectin-HUVEC Transfection<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
HBdMEC are isolated from the human<br />
bladder microvasculature. The cells are<br />
routinely analyzed by immunofluorescent<br />
staining: they stain positive for<br />
CD31 and von Willebrand factor and<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
negative for smooth muscle α-actin.<br />
HBdMEC are useful in studying the<br />
role of microvascular endothelial<br />
cells in the control of inflammation,<br />
capillary exchange, tissue growth, and<br />
angiogenesis.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Bladder Microvascular Endothelial<br />
<strong>Cell</strong>s (HBdMEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12291<br />
C-12292<br />
HBdMEC Pellet 1x10 6 cells in RNAlater ® C-14026<br />
Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s (HCMEC)<br />
HCMEC are isolated from heart ventricles<br />
from single donors. The cells are<br />
routinely analyzed by immunofluorescent<br />
staining: they stain positive for<br />
CD31 and von Willebrand factor and<br />
negative for smooth muscle α-actin.<br />
HCMEC intensively interact with cardiomyocytes<br />
and therefore have a characteristic<br />
phenotype different from other<br />
microvascular endothelial cells. HCMEC<br />
play an important role in the physiological<br />
regulation of coronary blood flow<br />
and capillary exchange. Medical conditions<br />
such as hypertrophy, ischemia,<br />
hypertension, and diabetes mellitus are<br />
associated with endothelial dysfunction.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
(see page 70)<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HCMEC Pellet (see page 97)<br />
Human Cardiac Myocytes (see page 9)<br />
Human Cardiac Fibroblasts<br />
(see page 26)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s (HCMEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12285<br />
C-12286<br />
HCMEC Pellet 1x10 6 cells in RNAlater ® C-14029
1 Human Primary <strong>Cell</strong>s 19<br />
Human Pulmonary Microvascular<br />
Endothelial <strong>Cell</strong>s (HPMEC)<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
Related products<br />
HPMEC Pellet (see page 98)<br />
(see page 70)<br />
Human Pulmonary Fibroblasts<br />
HPMEC are isolated from the lung from<br />
a single donor. The cells are routinely<br />
analyzed by immunofluorescent staining:<br />
they stain positive for CD31 and<br />
von Willebrand factor and negative for<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
(see page 26)<br />
Human Small Airway Epithelial <strong>Cell</strong>s<br />
(see page 22)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
smooth muscle α-actin.<br />
CD31 Antibody<br />
The lung has a vast endothelial surface<br />
(see www.promokine.info)<br />
area, which is essential for the exchange<br />
of gases. HPMEC are most appropriate<br />
for studying human lung diseases.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Pulmonary Microvascular<br />
Endothelial <strong>Cell</strong>s (HPMEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12281<br />
C-12282<br />
HPMEC Pellet 1x10 6 cells in RNAlater ® C-14027<br />
Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s (HUtMEC)<br />
HUtMEC are isolated from the myometrium<br />
(middle layer of the uterine wall)<br />
from a single donor, who has not been<br />
pre-treated with hormones. The cells<br />
are routinely analyzed by immunofluorescent<br />
staining: they stain positive for<br />
CD31 and von Willebrand factor and<br />
negative for smooth muscle α-actin.<br />
During pregnancy HUtMEC propagate<br />
intensely and change their phenotype<br />
and expression pattern in response to<br />
sex steroids. This includes up-regulation<br />
of HUtMEC nitric oxide synthase activity,<br />
modulation of adhesion molecule<br />
expression, and an increase in endothelial<br />
cell survival and angiogenic activity.<br />
HUtMEC are involved in medical conditions<br />
such as uterine fibroma or adenomyosis.<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
(see page 70)<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(see page 70)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HUtMEC Pellet (see page 98)<br />
Human Uterine Fibroblasts<br />
(see page 27)<br />
Human Uterine Smooth Muscle <strong>Cell</strong>s<br />
(see page 39)<br />
PromoFectin-HUVEC Transfection<br />
Reagent (see PromoKine catalog)<br />
CD31 Antibody<br />
(see www.promokine.info)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s (HUtMEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12295<br />
C-12296<br />
HUtMEC Pellet 1x10 6 cells in RNAlater ® C-14028
20<br />
1 Human Primary <strong>Cell</strong>s<br />
Human Brain Microvascular<br />
Endothelial <strong>Cell</strong>s (HBMEC)<br />
Brain microvascular endothelial cells are<br />
highly specialized cells responsible for<br />
Therefore, Promo<strong>Cell</strong> HBMEC are well<br />
suited for studying brain endothelial cell<br />
the formation and functional properties<br />
functions like active transport systems<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human Brain Microvascular <strong>Cell</strong>s<br />
(HBMEC) are isolated from cortical<br />
tissue (see Fig. 8) from a single donor.<br />
They are routinely analyzed by flow<br />
cytometry and immunofluorescent stai-<br />
of the Blood-Brain Barrier (BBB). The<br />
BBB is characterized by cellular tight<br />
junctions and active transport systems<br />
which allow the selective transport of<br />
substances into the brain, and prevent<br />
and drug uptake. Furthermore, neuroinflammation<br />
studies as well as tight<br />
junction research can be performed<br />
with HBMEC.<br />
ning. The cells are positive for CD31 and<br />
the entrance of unwanted substances.<br />
von Willebrand factor and negative for<br />
smooth muscle α-actin and CD90.<br />
Cerebral cortex<br />
Thalamus<br />
Corpus callosum<br />
Hypothalamus<br />
Pituitary gland<br />
Cerebellum<br />
Spinal cord<br />
Fig. 8: Schematic structure of the brain<br />
Fig. 9: HBMEC in phase contrast.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Brain Microvascular Endothelial<br />
<strong>Cell</strong>s (HBMEC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
coming soon<br />
coming soon<br />
C-12287<br />
C-12288<br />
HBMEC Pellet 1x10 6 cells in RNAlater ® coming soon C-14032
1 Human Primary <strong>Cell</strong>s 21<br />
1.5 Epithelial <strong>Cell</strong>s<br />
Applications<br />
Drug uptake studies<br />
Toxicological research<br />
Wound healing research<br />
Oncology<br />
<strong>Cell</strong> differentiation studies<br />
Respiratory disease research<br />
Features<br />
Cytokeratin positive<br />
Available from different tissues<br />
Cryopreserved at second passage<br />
Typically, epithelial tissue is made of<br />
tightly packed cells with little intercellular<br />
space and is arranged in flat sheets<br />
forming effective barriers between the<br />
underlying tissues and the external environment.<br />
Promo<strong>Cell</strong> offers epithelial cells from different<br />
locations:<br />
Airways: Nasal mucosa, trachea, bronchi,<br />
and distal respiratory tract<br />
Breast: Mammary gland<br />
Kidney: Whole kidney and renal cortex<br />
Placenta: Amniotic membrane<br />
Fig. 1: HTEpC culture in phase contrast.<br />
Note the cuboidal shape typical for airway<br />
epithelial cells.<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
The epithelium is the interface between the<br />
body and the external environment. It covers<br />
all outside surfaces and inside lumina<br />
e.g. the inner lining of the respiratory<br />
tract. In addition, epithelial cells also make<br />
up exocrine and endocrine glands. The<br />
functions of epithelial cells are diverse and<br />
include absorption, secretion, protection,<br />
transcellular transport, and sensation.<br />
Available Epithelial <strong>Cell</strong>s:<br />
HNEpC (see page 21)<br />
HTEpC (see page 21)<br />
HBEpC (see page 22)<br />
HSAEpC (see page 22)<br />
HMEpC (see page 23)<br />
HREpC (see page 23)<br />
HRCEpC (see page 24)<br />
HPlEpC (see page 24)<br />
Fig. 2: HTEpC culture stained for cytokeratin.<br />
Nuclei counterstained with<br />
DAPI.<br />
Human Nasal Epithelial <strong>Cell</strong>s<br />
(HNEpC)<br />
HNEpC are isolated from normal human<br />
nasal mucosa and stain positive for cytokeratin.<br />
The epithelium in the nasal cavity cleans,<br />
humidifies, and warms inhaled air. In addition,<br />
it produces mucus, which binds<br />
particles that are subsequently transported<br />
to the pharynx by cilia on the<br />
epithelial cells. HNEpC are useful for in<br />
vitro studies of these processes.<br />
Recommended media & reagents<br />
Airway Epithelial <strong>Cell</strong> Growth Medium<br />
(see page 71)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HNEpC Pellet (see page 98)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Nasal Epithelial <strong>Cell</strong>s (HNEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12620<br />
C-12621<br />
HNEpC Pellet 1x10 6 cells in RNAlater ® C-14062<br />
Human Tracheal Epithelial <strong>Cell</strong>s<br />
(HTEpC)<br />
HTEpC are isolated from the surface<br />
epithelium of human trachea and stain<br />
positive for cytokeratin.<br />
The respiratory epithelia are responsible<br />
for the lubrication of the lungs,<br />
the maintenance of humidity, and the<br />
cleaning of the respiratory tract. They<br />
are an important target for drugs, toxins,<br />
and carcinogens. In addition, they<br />
are involved in many diseases such as<br />
cystic fibrosis. Consequently, HTEpC are<br />
useful for investigating the function and<br />
pathology of the respiratory system.<br />
Recommended media & reagents<br />
Airway Epithelial <strong>Cell</strong> Growth Medium<br />
(see page 71)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HTEpC Pellet (see page 98)<br />
Human Tracheal Smooth Muscle <strong>Cell</strong>s<br />
(see page 37)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)
22<br />
1 Human Primary <strong>Cell</strong>s<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human Tracheal Epithelial <strong>Cell</strong>s (HTEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12644<br />
C-12645<br />
HTEpC Pellet 1x10 6 cells in RNAlater ® C-14064<br />
Human Bronchial Epithelial <strong>Cell</strong>s<br />
(HBEpC)<br />
HBEpC are isolated from the surface<br />
epithelium of human bronchi and stain<br />
positive for cytokeratin.<br />
The respiratory epithelia are responsible<br />
for the lubrication of the lungs,<br />
the maintenance of humidity, and the<br />
cleaning of the respiratory tract. They<br />
are an important target for drugs, toxins,<br />
and carcinogens. In addition, they<br />
are involved in many diseases such as<br />
cystic fibrosis. Consequently, HBEpC are<br />
useful for investigating the function and<br />
pathology of the respiratory system.<br />
Recommended media & reagents<br />
Airway Epithelial <strong>Cell</strong> Growth Medium<br />
(see page 71)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HBEpC Pellet (see page 97)<br />
Human Bronchial Smooth Muscle<br />
<strong>Cell</strong>s (see page 38)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Bronchial Epithelial <strong>Cell</strong>s (HBEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12640<br />
C-12641<br />
HBEpC Pellet 1x10 6 cells in RNAlater ® C-14063<br />
Human Small Airway Epithelial<br />
<strong>Cell</strong>s (HSAEpC)<br />
HSAEpC are isolated from the distal portion<br />
of the human respiratory tract in the<br />
1 mm bronchiole area. They stain positive<br />
for cytokeratin.<br />
The distal respiratory tract mainly consists<br />
of pulmonary alveoli, which are<br />
spherical outcroppings of the respiratory<br />
bronchioles and are the primary sites of<br />
gas exchange with the blood.<br />
HSAEpC are qualified for functional<br />
studies to investigate disorders such as<br />
asthma or pulmonary inflammation.<br />
Fig. 3: HSAEpC culture in phase contrast.<br />
Recommended media & reagents<br />
Small Airway Epithelial <strong>Cell</strong><br />
Growth Medium (see page 72)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HSAEpC Pellet (see page 98)<br />
Human Pulmonary Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 19)<br />
Human Pulmonary Fibroblasts<br />
(see page 26)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Small Airway Epithelial <strong>Cell</strong>s<br />
(HSAEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12642<br />
C-12643<br />
HSAEpC Pellet 1x10 6 cells in RNAlater ® C-14065
1 Human Primary <strong>Cell</strong>s 23<br />
Human Mammary Epithelial <strong>Cell</strong>s<br />
(HMEpC)<br />
milk secretion (lactation) begins.<br />
Since mammary gland cells can easily be<br />
Related products<br />
HMEpC Pellet (see page 97)<br />
induced to grow and multiply by hor-<br />
PromoFectin Transfection Reagent<br />
HMEpC are isolated from the human<br />
adult mammary gland and stain positive<br />
for cytokeratin.<br />
Hormones extensively control the development<br />
of the mammary glands.<br />
mones, HMEpC are very well suited for<br />
developmental investigations. In addition,<br />
they are useful for breast cancer in<br />
vitro research since breast cancer usually<br />
originates in the lobules or ducts of the<br />
(see PromoKine catalog)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Throughout puberty, the glands begin to<br />
mammary glands.<br />
develop in females in response to ovarian<br />
hormones. Further on during pregnancy,<br />
Recommended media & reagents<br />
rising levels of estrogen and progester-<br />
Mammary Epithelial <strong>Cell</strong> Growth<br />
one cause additional growth and differ-<br />
Medium (see page 73)<br />
entiation. Finally, due to the presence of<br />
DetachKit (see page 101)<br />
the hormone prolactin in late pregnancy,<br />
Cryo-SFM (see page 85)<br />
Fig. 4: HMEpC culture in phase contrast.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Mammary Epithelial <strong>Cell</strong>s<br />
(HMEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12650<br />
C-12651<br />
HMEpC Pellet 1x10 6 cells in RNAlater ® C-14066<br />
Human Renal Epithelial <strong>Cell</strong>s<br />
(HREpC)<br />
HREpC are isolated from the adult kidney<br />
and stain positive for cytokeratin.<br />
Kidneys have a very complex structure<br />
and contain diverse epithelia such as<br />
those in the convoluted tubules, the<br />
cortical collecting ducts, the calyxes,<br />
Renal cortex<br />
and the renal pelvis. The epithelial cells<br />
present in the distinct ducts and tubules<br />
differ in their morphology and physiological<br />
function. Consequently, HREpC<br />
are comprised of a heterogeneous population<br />
of epithelial cells.<br />
Important physiological processes like<br />
osmoregulation and excretion can be<br />
studied with HREpC in vitro.<br />
Recommended media & reagents<br />
Renal Epithelial <strong>Cell</strong> Growth<br />
Medium 2 (see page 74)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HREpC Pellet (see page 98)<br />
Human Renal Cortical Epithelial <strong>Cell</strong>s<br />
(see page 24)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
Renale capsule<br />
Renal medulla<br />
Renal calices<br />
Renal pelvis<br />
Ureter<br />
Fig. 5: Schematic structure of a kidney.<br />
Fig. 6: HREpC culture in phase contrast.
24<br />
1 Human Primary <strong>Cell</strong>s<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human Renal Epithelial <strong>Cell</strong>s (HREpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12665<br />
C-12666<br />
HREpC Pellet 1x10 6 cells in RNAlater ® C-14067<br />
Human Renal Cortical Epithelial<br />
<strong>Cell</strong>s (HRCEpC)<br />
HRCEpC are isolated from the cortex of<br />
the human kidney and stain positive for<br />
cytokeratin. The renal cortex is the outer<br />
portion of the kidney between the renal<br />
capsule and the renal medulla. It contains<br />
the renal corpuscles, the proximal<br />
and distal convoluted tubules, and the<br />
cortical collecting ducts.<br />
Important physiological processes like<br />
osmoregulation and excretion can be<br />
studied with HRCEpC in vitro.<br />
Recommended media & reagents<br />
Renal Epithelial <strong>Cell</strong> Growth<br />
Medium 2 (see page 74)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HRCEpC Pellet (see page 98)<br />
Human Renal Epithelial <strong>Cell</strong>s<br />
(see page 23)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Renal Cortical Epithelial <strong>Cell</strong>s<br />
(HRCEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12660<br />
C-12662<br />
HRCEpC Pellet 1x10 6 cells in RNAlater ® C-14068<br />
Human Placental Epithelial <strong>Cell</strong>s<br />
(HPlEpC)<br />
HPlEpC are isolated from the amniotic<br />
membrane and stain positive for cytokeratin.<br />
Placental Epithelial <strong>Cell</strong>s are of fetal origin<br />
and form a single layer at the inner<br />
surface of the amnion. They protect the<br />
fetus against maternal leukocytes, neoplastic<br />
cells, and pathogens. <strong>Culture</strong>d<br />
HPlEpC are suitable for physiological and<br />
pathological studies of amniotic functions.<br />
Recommended media & reagents<br />
Placental Epithelial <strong>Cell</strong> Growth<br />
Medium (see page 75)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
Human Villous Mesenchymal<br />
Fibroblasts (see page 27)<br />
HPlEpC Pellet (see page 98)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Placental Epithelial <strong>Cell</strong>s (HPlEpC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12395<br />
C-12397<br />
HPIEpC Pellet 1x10 6 cells in RNAlater ® C-14069
1 Human Primary <strong>Cell</strong>s 25<br />
1.6 Fibroblasts<br />
Applications<br />
Wound healing research<br />
Tissue engineering research<br />
Regeneration studies<br />
Toxicological research<br />
Oncology<br />
Epithelial-mesenchymal interaction<br />
studies<br />
Features<br />
CD90 positive<br />
Cryopreserved at second passage<br />
Available from different tissues<br />
Fibroblasts are the most common cells<br />
in connective tissue. Their main function<br />
is to maintain the structural integrity of<br />
the connective tissue by continuously<br />
secreting extracellular matrix proteins<br />
like collagens, glycosaminoglycans,<br />
and glycoproteins. The composition of<br />
the extracellular matrix determines the<br />
physical properties of connective tissues.<br />
Fibroblasts are morphologically heterogeneous<br />
with diverse appearances<br />
depending on their location in the body<br />
and their activity. Injury of tissue displays<br />
a proliferative stimulus for fibroblasts and<br />
induces them to produce wound healing<br />
proteins. Therefore, fibroblasts are well<br />
suited for wound healing studies.<br />
Available Fibroblasts:<br />
NHDF (see page 25)<br />
HPF (see page 26)<br />
HAoAF (see page 26)<br />
HCF (see page 26)<br />
HUF (see page 27)<br />
HVMF (see page 27)<br />
Fig. 1: NHDF culture in phase contrast.<br />
Fig. 2: NHDF culture stained for CD90.<br />
Nuclei counterstained with DAPI.<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Normal Human Dermal Fibroblasts<br />
(NHDF)<br />
NHDF are isolated from the dermis of<br />
juvenile foreskin or adult skin from different<br />
locations like the face, the breasts,<br />
the abdomen, and the thighs*. They can<br />
be used for wound healing studies and<br />
dermatological research to investigate<br />
diseases like scleroderma, fibrosarcoma,<br />
fibrosis, xeroderma pigmentosum, and<br />
histiocytoma. Moreover, fibroblasts are<br />
important for cancer research, tissue<br />
regeneration, and tissue engineering<br />
studies.<br />
Recommended media & reagents for<br />
NHDF juvenile foreskin<br />
Fibroblast Growth Medium<br />
(see page 76)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Recommended media & reagents for<br />
NHDF adult donor<br />
Fibroblast Growth Medium 2<br />
(see page 76)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
NHDF Pellet (see page 98)<br />
NHDF adult Pellet (see page 98)<br />
Normal Human Epidermal<br />
Keratinocytes (see page 30)<br />
Normal Human Epidermal<br />
Melanocytes (see page 31)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
FGF-1 and FGF-2 (see page 106)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Normal Human Dermal Fibroblasts (NHDF)<br />
juvenile foreskin<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12300<br />
C-12350<br />
NHDF Pellet 1x10 6 cells in RNAlater ® C-14030<br />
Normal Human Dermal Fibroblasts (NHDF)<br />
adult donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12302<br />
C-12352<br />
NHDF adult Pellet 1x10 6 cells in RNAlater ® C-14031<br />
* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are<br />
available on request.
26<br />
1 Human Primary <strong>Cell</strong>s<br />
Human Pulmonary Fibroblasts<br />
(HPF)<br />
pulmonary hypertension can also be investigated<br />
with cultured HPF.<br />
Related products<br />
HPF Pellet (see page 98)<br />
Human Pulmonary Microvascular<br />
HPF are isolated from human lung tis-<br />
Recommended media & reagents<br />
Endothelial <strong>Cell</strong>s (see page 19)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
sue. They are often used for studying<br />
lung tissue repair, tissue remodeling after<br />
injury, or the response after pulmonary<br />
inflammation. Fibroblast disorders like<br />
pulmonary fibrosis or hypoxia-induced<br />
Fibroblast Growth Medium 2<br />
(see page 76)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Human Small Airway Epithelial <strong>Cell</strong>s<br />
(see page 22)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
FGF-1 and FGF-2 (see page 106)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Pulmonary Fibroblasts (HPF)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12360<br />
C-12361<br />
HPF Pellet 1x10 6 cells in RNAlater ® C-14035<br />
Human Aortic Adventitial<br />
Fibroblasts (HAoAF)<br />
HAoAF are isolated from human aortic<br />
adventitial tissue. HAoAF can be used<br />
for studies concerning vascular diseases<br />
e.g. neointimal formation, vascular remodeling,<br />
atherosclerosis, and hypertension.<br />
They are also often used for the<br />
examination of fibroblast disorders like<br />
fibrosis or other diseases linked to either<br />
imperfect or excessive accumulation of<br />
fibroblasts.<br />
Recommended media & reagents<br />
Fibroblast Growth Medium 2<br />
(see page 76)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HAoAF Pellet (see page 97)<br />
Human Aortic Endothelial <strong>Cell</strong>s<br />
(see page 13)<br />
Human Aortic Smooth Muscle <strong>Cell</strong>s<br />
(see page 35)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
FGF-1 and FGF-2 (see page 106)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Aortic Adventitial Fibroblasts<br />
(HAoAF)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12380<br />
C-12381<br />
HAoAF Pellet 1x10 6 cells in RNAlater ® C-14037<br />
Human Cardiac Fibroblasts (HCF)<br />
HCF are isolated from the ventricles of<br />
an adult heart. They play a central role in<br />
the maintenance of the extracellular matrix<br />
in the normal heart and the synthesis<br />
of growth factors and cytokines. Under<br />
pathophysiological conditions, cardiac<br />
fibroblasts are involved in scar formation<br />
after myocardial infarction, cardiac fibrosis,<br />
and cardiac hypertrophy. HCF can be<br />
used to study such processes in vitro.<br />
Recommended media & reagents<br />
Fibroblast Growth Medium 3<br />
(see page 77)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HCF Pellet (see page 97)<br />
Human Cardiac Myocytes (see page 9)<br />
Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 18)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
FGF-1 and FGF-2 (see page 106)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Cardiac Fibroblasts (HCF)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12375<br />
C-12377<br />
HCF Pellet 1x10 6 cells in RNAlater ® C-14036
1 Human Primary <strong>Cell</strong>s 27<br />
Human Uterine Fibroblasts (HUF)<br />
Recommended media & reagents<br />
Related products<br />
Fibroblast Growth Medium 2<br />
HUF Pellet (see page 98)<br />
HUF are isolated from the uterine wall.<br />
(see page 76)<br />
Human Uterine Microvascular<br />
During pregnancy, the uterine wall un-<br />
DetachKit (see page 101)<br />
Endothelial <strong>Cell</strong> (see page 19)<br />
dergoes deep but reversible structural<br />
and biochemical changes. The uterine<br />
wall is the target of hormones and<br />
growth factors that act simultaneously<br />
and lead to extensive modifications of<br />
Cryo-SFM (see page 85)<br />
Human Uterine Smooth Muscle <strong>Cell</strong>s<br />
(see page 39)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
FGF-1 and FGF-2 (see page 106)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
the extracellular matrix. This and other<br />
morphological and biochemical effects<br />
can be studied in vitro using HUF.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Uterine Fibroblasts (HUF)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12385<br />
C-12386<br />
HUF Pellet 1x10 6 cells in RNAlater ® C-14038<br />
Human Villous Mesenchymal<br />
Fibroblasts (HVMF)<br />
HVMF are isolated from human villous<br />
placental tissue. They can be used as<br />
model systems in organ development<br />
research. In addition, they are useful for<br />
investigating the mechanism regulating<br />
human placental growth and function.<br />
Recommended media & reagents<br />
Fibroblast Growth Medium 2<br />
(see page 76)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HVMF Pellet (see page 98)<br />
Human Placental Epithelial <strong>Cell</strong>s<br />
(see page 24)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
FGF-1 and FGF-2 (see page 106)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Villous Mesenchymal Fibroblasts<br />
(HVMF)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12390<br />
C-12391<br />
HVMF Pellet 1x10 6 cells in RNAlater ® C-14034
28<br />
1 Human Primary <strong>Cell</strong>s<br />
1.7 Follicle Dermal Papilla <strong>Cell</strong>s<br />
Applications<br />
Recommended media & reagents<br />
Follicle Dermal Papilla <strong>Cell</strong> Growth<br />
Hair growth research<br />
Medium (see page 78)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Drug testing<br />
Toxicological testing<br />
Features<br />
Alkaline phosphatase positive<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HFDPC Pellet (see page 97)<br />
PromoFectin Transfection Reagent<br />
Fig. 1: HFDPC culture in phase contrast.<br />
Cryopreserved at second passage<br />
(see PromoKine catalog)<br />
Available from adult lateral scalp<br />
Available from donors with different<br />
hair colors<br />
Human Follicle Dermal Papilla <strong>Cell</strong>s<br />
(HFDPC) are isolated from human der-<br />
Sebaceous gland<br />
mis originating from the lateral scalp.<br />
Information on donor hair and skin color<br />
is available for each lot. HFDPC stain<br />
Hair shaft<br />
Bulge<br />
positive for alkaline phosphatase.<br />
Dermal papillae are embedded in a<br />
laminin and collagen IV rich extracellular<br />
Matrix cells<br />
matrix at the base of the hair follicles.<br />
They are essential for the induction and<br />
maintenance of hair growth. Since they<br />
have androgen receptors, they can be<br />
Dermal papilla<br />
used for in vitro screening of androgen<br />
blocking reagents.<br />
Fig. 2: Diagram showing the location of the dermal papilla in the hair follicle.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Follicle Dermal Papilla <strong>Cell</strong>s<br />
(HFDPC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12071<br />
C-12072<br />
HFDPC Pellet 1x10 6 cells in RNAlater ® C-14005
1 Human Primary <strong>Cell</strong>s 29<br />
1.8 Hepatocytes<br />
Applications<br />
metabolism, and albumin synthesis. The<br />
Recommended media & reagents<br />
cell adherence efficiency is at least 50%.<br />
Hepatocyte Growth Medium<br />
Drug testing<br />
(see page 79)<br />
Toxicological testing<br />
Metabolism studies<br />
Liver physiology research<br />
Liver pathology research<br />
Oncology<br />
The liver is the main metabolic organ<br />
in the body performing glycolysis,<br />
glycogenesis, amino acid metabolism,<br />
lipid metabolism, and ureagenesis. In<br />
addition, it also regulates the blood<br />
Hepatocyte Maintenance Medium<br />
(see page 79)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Features<br />
sugar level. Furthermore, hepatocytes<br />
eliminate toxic substances soon after<br />
Related products<br />
HHpC Pellet (see page 97)<br />
absorption since the nutrition rich blood<br />
PromoFectin-Hepatocyte Transfection<br />
Cryopreserved immediately after<br />
from the gastro-intestinal system first<br />
Reagent (see PromoKine catalog)<br />
isolation<br />
has to pass the liver. This detoxifica-<br />
Collagen Type I from Rat Tail<br />
3 - 6 x 10 6 viable cells per cryovial<br />
tion consists of phase I and phase II<br />
(see page 105)<br />
Adherence > 50%<br />
reactions. The most important phase I<br />
Phase I reaction positive<br />
enzyme system is the cytochrome P450<br />
Phase II reaction positive<br />
system. Consequently, cultured human<br />
Albumin positive<br />
hepatocytes are perfectly suited for in<br />
vitro metabolism and toxicity/detoxi-<br />
Human Hepatocytes (HHpC) are iso-<br />
fication studies prior to preclinical or<br />
lated from the normal livers of single<br />
clinical tests.<br />
donors. After isolation, the cells are<br />
immediately cryopreserved and then<br />
Note: Under standard in vitro cell cul-<br />
intensively quality tested. Test cri-<br />
ture conditions, mature human hepato-<br />
teria are cell viability, cell adherence<br />
efficiency, phase I metabolism, phase II<br />
cytes usually do not survive for longer<br />
than 10-14 days and do not proliferate.<br />
Fig 1. HHpC culture in phase contrast.<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
Count<br />
37,5<br />
Living cells<br />
Dead cells<br />
Fig. 2: Flow cytometric analysis of Promo<strong>Cell</strong> HHpC<br />
using 7-AAD staining. 77 % viable hepatocytes after<br />
thawing of the cryopreserved cells.<br />
25<br />
12,5<br />
0<br />
0<br />
10 10 2 10 3 10 4<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
7-AAD<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Hepatocytes (HHpC) 3 - 6 x 10 6 cryopreserved cells C-12850<br />
HHpC Pellet 1x10 6 cells in RNAlater ® C-14085
30<br />
1 Human Primary <strong>Cell</strong>s<br />
1.9 Keratinocytes<br />
Applications<br />
body and minimizes moisture loss.<br />
Related products<br />
Keratinocytes are also able to produce<br />
NHEK.f Pellet (see page 98)<br />
Wound healing research<br />
a variety of cytokines, growth factors,<br />
NHEK.f pooled Pellet (see page 98)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Epidermal development and<br />
differentiation studies<br />
Dermatological research<br />
Drug uptake studies<br />
Pharmaceutical testing<br />
interleukins and complement factors.<br />
Therefore keratinocytes are important<br />
for wound healing, inflammation, and<br />
immune response.<br />
NHEK adult Pellet (see page 98)<br />
NHEK adult, pooled Pellet<br />
(see page 98)<br />
Normal Human Dermal Fibroblasts<br />
(see page 25)<br />
Cosmetic and toxicological testing<br />
Recommended media & reagents<br />
Normal Human Epidermal<br />
Oncology<br />
Keratinocyte Growth Medium 2<br />
Melanocytes (see page 31)<br />
Features<br />
(see page 80)<br />
DetachKit (see page 101)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
Cryo-SFM (see page 85)<br />
Cytokeratin positive<br />
Available from juvenile foreskin or<br />
adult skin from different locations<br />
Available from single and pooled<br />
Stratum corneum<br />
Stratum granulosum<br />
donors<br />
Stratum spinosum<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) are available from single or<br />
pooled donors isolated from the epidermis<br />
Stratum basale<br />
of juvenile foreskin or adult skin from different<br />
locations like the face, the breasts,<br />
the abdomen, and the thighs*. They are<br />
Fig. 1: Structure of the epidermis.<br />
the major cell type in the epidermis, making<br />
up about 90% of the cells.<br />
Epidermal keratinocytes originate in the<br />
stratum basale and move up through<br />
the layers of the epidermis. During this<br />
movement, they undergo gradual differentiation<br />
and morphology changes until<br />
they reach the stratum corneum, where<br />
they form a layer of nucleus-free, flat,<br />
and highly keratinized squamous cells.<br />
Fig. 2: NHEK culture in phase contrast.<br />
Fig. 3: NHEK culture stained for Cyto-<br />
This layer forms an effective barrier to<br />
Note the cobblestone shape typical for<br />
keratin. Nuclei counterstained with<br />
the entry of infectious agents into the<br />
keratinocytes.<br />
DAPI.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) juvenile foreskin, single donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12001<br />
C-12002<br />
NHEK.f Pellet 1x10 6 cells in RNAlater ® C-14001<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) juvenile foreskin, pooled<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12005<br />
C-12007<br />
NHEK.f pooled Pellet 1x10 6 cells in RNAlater ® new<br />
C-14003<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) adult, single donor<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12003<br />
C-12004<br />
NHEK adult Pellet 1x10 6 cells in RNAlater ® C-14002<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) adult, pooled<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12006<br />
C-12008<br />
NHEK adult, pooled Pellet 1x10 6 cells in RNAlater ® new<br />
C-14004<br />
* Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available<br />
on request.<br />
new<br />
new<br />
new<br />
new
1 Human Primary <strong>Cell</strong>s 31<br />
1.10 Melanocytes<br />
Applications<br />
using either the serum-free, PMA (Phor-<br />
Melanocyte Growth Medium M2<br />
bol Myristate Acetate)-containing Mela-<br />
(see page 81)<br />
Dermatological research<br />
nocyte Growth Medium or the serum-<br />
DetachKit (see page 101)<br />
Melanoma research<br />
Melanogenesis studies<br />
Cosmetic and toxicological testing<br />
Skin compound screening<br />
Features<br />
free and PMA-free Melanocyte Growth<br />
Medium M2.<br />
Since PMA is a tumor promoting mitogen,<br />
it can interfere with experimental<br />
approaches. Therefore, we recommend<br />
using cells isolated in Melanocyte<br />
Cryo-SFM (see page 85)<br />
Related products<br />
NHEM.f Pellet (see page 98)<br />
NHEM.f M2 Pellet (see page 98)<br />
NHEM M2 adult Pellet (see page 98)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Growth Medium M2 and also using this<br />
Normal Human Dermal Fibroblasts<br />
Available from juvenile foreskin or<br />
medium for cultivation.<br />
(see page 25)<br />
adult skin from different locations<br />
Normal Human Epidermal<br />
Available from light, moderate, and<br />
Recommended media & reagents<br />
Keratinocytes (see page 30)<br />
dark skin types<br />
Melanocyte Growth Medium<br />
PromoFectin Transfection Reagent<br />
Donor hair and eye color available on<br />
(see page 81)<br />
(see PromoKine catalog)<br />
request<br />
Mel-5 positive<br />
<strong>Culture</strong>d in PMA-containing or<br />
PMA-free Medium<br />
Stratum corneum<br />
Stratum granulosum<br />
Normal Human Epidermal Melanocytes<br />
(NHEM) are isolated from the epidermis<br />
Stratum spinosum<br />
of juvenile foreskin or adult skin from<br />
different locations including the face, the<br />
breasts, the abdomen, and the thighs*.<br />
They are located in the stratum basale,<br />
Stratum basale<br />
but branch out between the keratinocytes<br />
in suprabasal layers (see Fig. 1).<br />
About 5-10 % of the cells in the epidermis<br />
are melanocytes.<br />
Melanosoma Keratinocytes<br />
Melanocyte<br />
Fig. 1: Structure of the epidermis with<br />
the location of a melanocyte.<br />
The main purpose of melanocytes is<br />
the production of melanin, the protein<br />
responsible for the pigmentation of the<br />
skin, eyes, and hair. Melanin protects the<br />
cells in the skin and in deeper layers from<br />
the hazardous effects of UV radiation. It<br />
is produced and stored in melanosomes,<br />
which are located close to the melanocyte<br />
membrane, but it can also be transferred<br />
to neighboring keratinocytes.<br />
Fig. 2: NHEM cultured in Melanocyte<br />
Fig. 3: NHEM cultured in Melanocyte<br />
Promo<strong>Cell</strong> melanocytes are isolated<br />
Growth Medium in phase contrast.<br />
Growth Medium M2 in phase contrast.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Normal Human Epidermal Melanocytes<br />
(NHEM) juvenile foreskin<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12400<br />
C-12450<br />
NHEM.f Pellet 1x10 6 cells in RNAlater ® C-14040<br />
Normal Human Epidermal Melanocytes<br />
(NHEM) juvenile foreskin, cultured in M2<br />
Medium<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12402<br />
C-12452<br />
NHEM.f M2 Pellet 1x10 6 cells in RNAlater ® C-14042<br />
Normal Human Epidermal Melanocytes<br />
(NHEM) adult donor, cultured in M2<br />
Medium<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12403<br />
C-12453<br />
NHEM M2 adult Pellet 1x10 6 cells in RNAlater ® C-14043<br />
* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.
32<br />
1 Human Primary <strong>Cell</strong>s<br />
1.11 Osteoblasts<br />
Applications<br />
Human Osteoblasts (HOB) are isolated<br />
Recommended media & reagents<br />
from femoral trabecular bone tissue from<br />
Osteoblast Growth Medium<br />
Bone differentiation<br />
the knee or hip joint region (lot specific<br />
(see page 82)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Bone remodeling<br />
Bone physiology research<br />
Oncology<br />
Inflammatory studies<br />
Osteoporosis research<br />
source information is available on request).<br />
They stain positive for alkaline<br />
phosphatase and can be mineralized using<br />
Promo<strong>Cell</strong> Mineralization Medium.<br />
Osteoblasts are of mesenchymal origin<br />
Osteoblast Mineralization Medium<br />
(see page 82)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Features<br />
and are mainly responsible for bone formation<br />
and remodeling. In vivo, they<br />
Related products<br />
HOB Pellet (see page 98)<br />
produce a collagen type I rich extracellular<br />
PromoFectin Transfection Reagent<br />
From knee and hip femoral tissue<br />
matrix called the osteoid.<br />
(see PromoKine catalog)<br />
Cryopreserved at second passage<br />
During their development, osteoblasts<br />
Alkaline phosphatase positive<br />
undergo a maturation process called<br />
Tested for mineralization<br />
osteogenesis. When they become en-<br />
Growth and Mineralization Media<br />
cased in the bone matrix, they stop prolif-<br />
available<br />
erating and become osteocytes.<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
Fig. 1: HOB culture in phase contrast.<br />
Fig. 2: Alkaline phosphatase activity in<br />
HOB stained with BCIP/NBT.<br />
Fig. 3: Mineralized HOB. Calcium deposits<br />
stained with Alizarin Red S.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Osteoblasts (HOB)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12720<br />
C-12760<br />
HOB Pellet 1x10 6 cells in RNAlater ® C-14071
1 Human Primary <strong>Cell</strong>s 33<br />
1.12 Preadipocytes<br />
Applications<br />
Adipose tissue is crucial in energy storage<br />
Recommended media & reagents<br />
and metabolic homeostasis. An increase<br />
Preadipocyte Growth Medium<br />
Proliferation/differentiation studies<br />
in adipose tissue results either from an<br />
(see page 83)<br />
Physiological and pathological<br />
research<br />
Fat metabolism research<br />
Obesity/diabetes studies<br />
Oncology (e.g. breast cancer)<br />
enlargement of mature adipocytes or<br />
from the differentiation of adipocyte<br />
precursor cells (preadipocytes) into new<br />
mature adipocytes. These preadipocytes<br />
are already present in adipose tissues.<br />
Preadipocyte Differentiation Medium<br />
(see page 83)<br />
Adipocyte Nutrition Medium<br />
(see page 83)<br />
DetachKit (see page 101)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Features<br />
They can proliferate throughout adult<br />
life and replace the cells that have dif-<br />
Cryo-SFM (see page 85)<br />
ferentiated into mature adipocytes.<br />
Related products<br />
From subcutaneous and visceral<br />
Since this differentiation process is con-<br />
HWP subcutaneous Pellet<br />
adipose tissue<br />
trolled by a variety of growth factors and<br />
(see page 98)<br />
Available from different locations<br />
hormones, preadipocytes are suitable<br />
HWP visceral Pellet (see page 98)<br />
Cryopreserved at second passage<br />
for the investigation of the physiologi-<br />
PromoFectin Transfection Reagent<br />
Differentiation capacity to adipocytes<br />
cal mechanisms controlling proliferation,<br />
(see PromoKine catalog)<br />
Growth and Differentiation Media<br />
differentiation, and function of adipose<br />
available<br />
tissue.<br />
Human White Preadipocytes (HWP) are<br />
The Promo<strong>Cell</strong> adipocyte cell culture<br />
isolated from adult subcutaneous or vis-<br />
system provides preadipocytes with op-<br />
ceral adipose tissue from different loca-<br />
timized growth media and serum-free<br />
tions (lot specific source information is<br />
differentiation media.<br />
available on request).<br />
Fig. 1: Left: Undifferentiated HWP (phase contrast). Middle: HWP after in vitro differentiation into adipocytes using the Promo<strong>Cell</strong><br />
Preadipocyte Differentiation Medium (phase contrast). Right: HWP after in vitro differentiation into adipocytes (Lipid droplets<br />
stained with Oil Red O).<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human White Preadipocytes (HWP)<br />
subcutaneous<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12730<br />
C-12731<br />
HWP subcutaneous Pellet 1x10 6 cells in RNAlater ® C-14072<br />
Human White Preadipocytes (HWP)<br />
visceral<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12732<br />
C-12733<br />
HWP visceral Pellet 1x10 6 cells in RNAlater ® C-14073
34<br />
1 Human Primary <strong>Cell</strong>s<br />
1.13 Skeletal Muscle <strong>Cell</strong>s<br />
Applications<br />
liferate and fuse with damaged muscle<br />
Recommended media & reagents<br />
fibers or with one another forming new<br />
Skeletal Muscle <strong>Cell</strong> Growth Medium<br />
Gene therapy research<br />
myotubes.<br />
(see page 84)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Transplantation research<br />
Developmental/differentiation<br />
studies<br />
Tissue repair research<br />
Pharmacological testing<br />
SkMC are optimal for in vitro muscle<br />
studies. They proliferate very well in<br />
the mitogen-rich Promo<strong>Cell</strong> Skeletal<br />
Muscle <strong>Cell</strong> Growth Medium. Fusion to<br />
Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium (see page 84)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Neuromuscular disease research<br />
myotubes with typical multinucleated<br />
Related products<br />
Features<br />
syncytia can be induced by using the<br />
Promo<strong>Cell</strong> Skeletal Muscle <strong>Cell</strong> Differen-<br />
SkMC Pellet (see page 98)<br />
PromoFectin Transfection Reagent<br />
tiation Medium.<br />
(see PromoKine catalog)<br />
Sarcomeric myosin positive<br />
Available from different muscles<br />
Differentiation capacity to multi-<br />
nucleate syncytia<br />
Growth and Differentiation Media<br />
available<br />
Human Skeletal Muscle <strong>Cell</strong>s (SkMC) are<br />
isolated from different skeletal muscles<br />
(e.g. Musculus pectoralis major) from<br />
Fig. 1: SkMC culture in phase contrast.<br />
adult single donors (lot specific source<br />
information is available on request).<br />
They are positive for sarcomeric myosin<br />
and negative for smooth muscle specific<br />
α-actin.<br />
New skeletal muscle cells originate from<br />
quiescent satellite cells, which are located<br />
in the muscle fibers between the basal<br />
lamina and the sarcolemma. Quiescent<br />
satellite cells are activated by stimuli such<br />
Fig. 2: Differentiated SkMC culture in<br />
as muscle damage. After activation, the<br />
phase contrast. Arrows indicate multi-<br />
cells, now called myoblasts, start to pro-<br />
nucleated syncytia.<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Skeletal Muscle <strong>Cell</strong>s (SkMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12530<br />
C-12580<br />
SkMC Pellet 1x10 6 cells in RNAlater ® C-14060
1 Human Primary <strong>Cell</strong>s 35<br />
1.14 Smooth Muscle <strong>Cell</strong>s<br />
Applications<br />
the walls of blood vessels and hollow organs<br />
like the bladder, the uterus, and the<br />
Organ specific drug testing<br />
gastrointestinal tract and are responsible<br />
<strong>Cell</strong>-extracellular matrix interaction<br />
studies<br />
Physiological and biochemical studies<br />
Oncology<br />
Pathological studies<br />
(e.g. hypertension)<br />
for involuntary movements like peristaltic<br />
contractions, which propel food along<br />
the gastrointestinal tract.<br />
Promo<strong>Cell</strong> Smooth Muscle <strong>Cell</strong>s are<br />
available from the aorta, the coronary<br />
artery, the pulmonary artery, the umbili-<br />
Fig. 1: HTSMC culture in phase contrast.<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Atherosclerosis research<br />
cal artery, the trachea, the bronchi, the<br />
bladder, the prostate, and the uterus.<br />
Features<br />
Available Smooth Muscle <strong>Cell</strong>s:<br />
Smooth muscle α-actin positive<br />
HAoSMC (see page 35)<br />
CD90 negative<br />
HCASMC (see page 36)<br />
Routinely morphology tested<br />
HPASMC (see page 36)<br />
Cryopreserved at second passage<br />
HUASMC (see page 37)<br />
HTSMC (see page 37)<br />
Smooth muscle cells, which form non-<br />
HBSMC (see page 38)<br />
striated muscles, are structurally and<br />
HBdSMC (see page 38)<br />
Fig. 2: HTSMC culture stained for smooth<br />
functionally different from skeletal and<br />
HPrSMC (see page 39)<br />
muscle α-actin. Nuclei counterstained<br />
cardiac muscle cells. They are located in<br />
HUtSMC (see page 39)<br />
with DAPI.<br />
Human Aortic Smooth Muscle<br />
<strong>Cell</strong>s (HAoSMC)<br />
HAoSMC are isolated from plaque-free<br />
regions of the human aorta (see Fig. 3)<br />
and stain positive for smooth muscle<br />
α-actin. The pulsatile pressure produced<br />
by the heart causes a cyclic distention<br />
Tunica externa<br />
(Connective tissue)<br />
of the aorta. The smooth muscle cells in<br />
the aortic wall subsequently contract it<br />
again. Changes in the arterial wall, associated<br />
with vascular diseases such as ath-<br />
Tunica media<br />
(Smooth muscle cells)<br />
erosclerosis and hypertension, strongly<br />
influence this process.<br />
HAoSMC are suitable for studying the<br />
role of smooth muscle cells under normal<br />
or disease conditions in vitro.<br />
Tunica intima<br />
(Endothelial cells)<br />
Fig. 3: Diagram of a blood vessel.<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Related products<br />
Human Aortic Endothelial <strong>Cell</strong>s<br />
Medium 2 (see page 85)<br />
HAoSMC Pellet (see page 97)<br />
(see page 13)<br />
DetachKit (see page 101)<br />
Human Aortic Adventitial Fibroblasts<br />
PromoFectin Transfection Reagent<br />
Cryo-SFM (see page 85)<br />
(see page 26)<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Aortic Smooth Muscle <strong>Cell</strong>s<br />
(HAoSMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12533<br />
C-12532<br />
HAoSMC Pellet 1x10 6 cells in RNAlater ® C-14053
36<br />
1 Human Primary <strong>Cell</strong>s<br />
Human Coronary Artery Smooth<br />
Muscle <strong>Cell</strong>s (HCASMC)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
HCASMC are isolated from human coronary<br />
arteries (see Fig. 4) and stain positive<br />
for smooth muscle α-actin. Coronary<br />
arteries supply the heart with blood<br />
and obstruction in atherosclerosis causes<br />
myocardial infarction.<br />
Left main coronary<br />
artery<br />
Circumflex coronary<br />
artery<br />
HCASMC are qualified to study the<br />
function of smooth muscle cells in the<br />
development of atherosclerotic plaques<br />
or in other diseases like hypertension.<br />
They are also useful for stent-graft compatibility<br />
testing.<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Right coronary<br />
artery<br />
Fig. 4: Location of the coronary arteries.<br />
Related products<br />
HCASMC Pellet (see page 97)<br />
Human Coronary Artery Endothelial<br />
Left anterior descending<br />
coronary artery<br />
<strong>Cell</strong>s (see page 13)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Coronary Artery Smooth Muscle<br />
<strong>Cell</strong>s (HCASMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12511<br />
C-12512<br />
HCASMC Pellet 1x10 6 cells in RNAlater ® C-14052<br />
Human Pulmonary Artery Smooth<br />
Muscle <strong>Cell</strong>s (HPASMC)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
HPASMC are isolated from human pulmonary<br />
arteries and stain positive for<br />
smooth muscle α-actin. They are suited<br />
for studying the function of pulmonary<br />
arteries under normal and disease conditions<br />
(e.g. vasoconstriction induced<br />
by alveolar hypoxia). They are also useful<br />
for physiological studies of vascular<br />
smooth muscle cells.<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
Related products<br />
HPASMC Pellet (see page 98)<br />
Human Pulmonary Artery Endothelial<br />
<strong>Cell</strong>s (see page 14)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
Fig. 5: Top: HPASMC culture in phase<br />
contrast. Bottom: HPASMC culture<br />
stained for smooth muscle α-actin.<br />
Nucleus counterstained with DAPI.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Pulmonary Artery Smooth Muscle<br />
<strong>Cell</strong>s (HPASMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12521<br />
C-12522<br />
HPASMC Pellet 1x10 6 cells in RNAlater ® C-14051
1 Human Primary <strong>Cell</strong>s 37<br />
Human Umbilical Artery Smooth<br />
Muscle <strong>Cell</strong>s (HUASMC)<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Related products<br />
HUASMC Pellet (see page 98)<br />
Medium 2 (see page 85)<br />
Human Umbilical Artery Endothelial<br />
HUASMC are isolated from the arteries<br />
of the umbilical cord and stain positive<br />
for smooth muscle α-actin. Physiological<br />
and biochemical processes of vascular<br />
diseases can be analyzed with HUASMC<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
<strong>Cell</strong>s (see page 12)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
in culture.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Umbilical Artery Smooth Muscle<br />
<strong>Cell</strong>s (HUASMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12500<br />
C-12550<br />
HUASMC Pellet 1x10 6 cells in RNAlater ® C-14050<br />
Human Tracheal Smooth Muscle<br />
<strong>Cell</strong>s (HTSMC)<br />
HTSMC are isolated from the trachea<br />
(see Fig. 6) from single donors and stain<br />
positive for smooth muscle α-actin. They<br />
connect the endings of the tracheal<br />
cartilage by a contractile bridge called<br />
the paries membranaceus and consequently<br />
regulate the airway lumen. Under<br />
pathological conditions like asthma,<br />
the amount of smooth muscle cells in<br />
the trachea walls is increased leading to<br />
smooth muscle hypertrophy or hyperplasia.<br />
Hence, HTSMC are suitable for in<br />
vitro studies of asthma and other pulmonary<br />
diseases.<br />
Paries<br />
membranaceus<br />
Hyaline cartilage<br />
Mucosa<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Related products<br />
HTSMC Pellet (see page 98)<br />
Fig. 6: Cross section through the trachea with c-shaped cartilage connected by the<br />
tracheal muscles.<br />
Human Tracheal Epithelial <strong>Cell</strong>s<br />
(see page 21)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Tracheal Smooth Muscle <strong>Cell</strong>s<br />
(HTSMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12565<br />
C-12566<br />
HTSMC Pellet 1x10 6 cells in RNAlater ® C-14056
38<br />
1 Human Primary <strong>Cell</strong>s<br />
Human Bronchial Smooth Muscle<br />
<strong>Cell</strong>s (HBSMC)<br />
leading to smooth muscle hypertrophy<br />
or hyperplasia. Hence, HBSMC are suit-<br />
Related products<br />
HBSMC Pellet (see page 97)<br />
able for in vitro studies of asthma and<br />
Human Bronchial Epithelial <strong>Cell</strong>s<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
HBSMC are isolated from the bronchi<br />
from single donors and stain positive for<br />
smooth muscle α-actin. They connect<br />
the endings of the bronchial cartilage<br />
and consequently regulate the airway lu-<br />
other pulmonary diseases.<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
(see page 22)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
men. Under pathological conditions like<br />
DetachKit (see page 101)<br />
asthma, the amount of smooth muscle<br />
Cryo-SFM (see page 85)<br />
cells in the bronchi walls is increased<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Bronchial Smooth Muscle <strong>Cell</strong>s<br />
(HBSMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12561<br />
C-12562<br />
HBSMC Pellet 1x10 6 cells in RNAlater ® C-14055<br />
Human Bladder Smooth Muscle<br />
<strong>Cell</strong>s (HBdSMC)<br />
HBdSMC are isolated from the detrusor<br />
muscle from the urinary bladder (see<br />
Fig. 7) of single donors and stain positive<br />
for smooth muscle α-actin. The thick<br />
bladder wall contains smooth muscle cells<br />
arranged in muscle bundles, which empty<br />
the bladder by coordinated contraction.<br />
HBdSMC can be used for in vitro studies<br />
of physiological processes as well as the<br />
effect of drugs on the bladder.<br />
Mucosa<br />
Submucosa<br />
Detrusor Muscle<br />
Adventitia<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Fig. 7: Diagram of the bladder.<br />
Related products<br />
HBdSMC Pellet (see page 97)<br />
Human Bladder Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 18)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Bladder Smooth Muscle <strong>Cell</strong>s<br />
(HBdSMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12571<br />
C-12572<br />
HBdSMC Pellet 1x10 6 cells in RNAlater ® C-14057
1 Human Primary <strong>Cell</strong>s 39<br />
Human Prostate Smooth Muscle<br />
<strong>Cell</strong>s (HPrSMC)<br />
HPrSMC are isolated from the prostate<br />
from single donors and stain positive<br />
for smooth muscle a-actin. The prostate<br />
produces secretions that get into<br />
the urethra through contraction of the<br />
smooth muscle cells.<br />
Prostate smooth muscle cells can be involved<br />
in prostitis and benign prostatic<br />
hyperplasia (BPH). HPrSMC can be used<br />
to study these prostate diseases or physiological<br />
processes in vitro.<br />
Lateral lobes<br />
Capsule<br />
Verumontanum<br />
Ejaculatory duct<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Fig. 8: Diagram of the prostate.<br />
Related products<br />
HPrSMC Pellet (see page 98)<br />
PromoFectin Transfection Reagent<br />
(see PromoKine catalog)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Prostate Smooth Muscle <strong>Cell</strong>s<br />
(HPrSMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12573<br />
C-12574<br />
HPrSMC Pellet 1x10 6 cells in RNAlater ® C-14058<br />
Human Uterine Smooth Muscle<br />
<strong>Cell</strong>s (HUtSMC)<br />
HUtSMC are isolated from the myometrium<br />
(the middle layer of the uterine wall,<br />
see Fig. 9) and stain positive for smooth<br />
muscle α-actin. During pregnancy, hormones<br />
and growth factors cause an extensive<br />
hypertrophy of uterine smooth<br />
muscle cells.<br />
This and other morphological and biochemical<br />
effects on the uterus can be<br />
studied in vitro using HUtSMC.<br />
Recommended media & reagents<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2 (see page 85)<br />
DetachKit (see page 101)<br />
Cryo-SFM (see page 85)<br />
Uterine cavity<br />
Vagina<br />
Fig. 9: Diagram of the uterus.<br />
Related products<br />
HUtSMC Pellet (see page 98)<br />
Uterine tube<br />
Perimetrium (peritoneum)<br />
Myometrium (muscle)<br />
Endometrium (epithelium)<br />
Cavity of cervix<br />
Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 19)<br />
Fig. 9: Diagram of the uterus.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Uterine Smooth Muscle <strong>Cell</strong>s<br />
(HUtSMC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12575<br />
C-12576<br />
HUtSMC Pellet 1x10 6 cells in RNAlater ® C-14059
40<br />
1 Human Primary <strong>Cell</strong>s<br />
Overview Human Primary <strong>Cell</strong>s<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Product<br />
Human Cardiac Myocytes<br />
(HCM)<br />
Cat. No.<br />
Cryopr. <strong>Cell</strong>s<br />
Recommended<br />
<strong>Culture</strong> Media*<br />
Plating Density<br />
C-12810 C-22070 10,000 - 15,000<br />
cells per cm²<br />
Human Chondrocytes (HCH) C-12710 C-27101 10,000 - 20,000<br />
cells per cm²<br />
Passage after<br />
Thawing<br />
Marker<br />
Population<br />
Doublings<br />
Page<br />
> 15 9<br />
P2 Sarcomeric α-actinin +<br />
myosin +<br />
Slow muscle<br />
P2 Differentiation tested > 10 10<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC)<br />
single donor<br />
C-12200 C-22010<br />
C-22011<br />
5,000 - 10,000<br />
cells per cm²<br />
P1 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 11<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC)<br />
pooled<br />
C-12203 C-22010<br />
C-22011<br />
5,000 - 10,000<br />
cells per cm²<br />
P1 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 11<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC)<br />
pre-screened<br />
C-12205 C-22010<br />
C-22011<br />
5,000 - 10,000<br />
cells per cm²<br />
P1 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 11<br />
Human Umbilical Artery<br />
Endothelial <strong>Cell</strong>s (HUAEC)<br />
C-12202 C-22010<br />
C-22011<br />
5,000 - 10,000<br />
cells per cm²<br />
P1 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 12<br />
Human Aortic<br />
Endothelial <strong>Cell</strong>s (HAoEC)<br />
C-12271 C-22020<br />
C-22022<br />
5,000 - 10,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 13<br />
Human Coronary Artery<br />
Endothelial <strong>Cell</strong>s (HCAEC)<br />
C-12221 C-22020<br />
C-22022<br />
5,000 - 10,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 13<br />
Human Pulmonary Artery<br />
Endothelial <strong>Cell</strong>s (HPAEC)<br />
C-12241 C-22010<br />
C-22011<br />
5,000 - 10,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 14<br />
Human Saphenous Vein<br />
Endothelial <strong>Cell</strong>s (HSaVEC)<br />
C-12231 C-22010<br />
C-22011<br />
5,000 - 10,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 14<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC)<br />
juvenile foreskin<br />
C-12210 C-22020<br />
C-22022<br />
10,000 - 20,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 15<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC)<br />
adult donor<br />
C-12212 C-22020<br />
C-22022<br />
10,000 - 20,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 15<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC)<br />
pre-screened<br />
C-12215 C-22020<br />
C-22022<br />
10,000 - 20,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 15<br />
Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s (HDBEC)<br />
juvenile foreskin<br />
C-12211 C-22020 10,000 - 20,000<br />
cells per cm²<br />
P2 Podoplanin -<br />
Prox-1 -<br />
CD31 +<br />
> 15 16<br />
Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s (HDBEC)<br />
adult donor<br />
C-12225 C-22020 10,000 - 20,000<br />
cells per cm²<br />
P2 Podoplanin -<br />
Prox-1 -<br />
CD31 +<br />
> 15 16<br />
Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s (HDLEC)<br />
juvenile foreskin<br />
C-12216 C-22022 10,000 - 20,000<br />
cells per cm²<br />
P2 Podoplanin +<br />
Prox-1 +<br />
CD31 +<br />
> 15 17<br />
Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s (HDLEC)<br />
adult donor<br />
C-12217 C-22022 10,000 - 20,000<br />
cells per cm²<br />
P2 Podoplanin +<br />
Prox-1 +<br />
CD31 +<br />
> 15 17<br />
Human Bladder Microvascular<br />
Endothelial <strong>Cell</strong>s (HBdMEC)<br />
C-12291 C-22020<br />
C-22022<br />
10,000 - 20,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 18<br />
Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s (HCMEC)<br />
C-12285 C-22020<br />
C-22022<br />
10,000 - 20,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 18<br />
* The media in this table are ready-to-use. For Media Kits see chapter 4.
1 Human Primary <strong>Cell</strong>s 41<br />
Product<br />
Cat. No.<br />
Cryopr. <strong>Cell</strong>s<br />
Recommended<br />
<strong>Culture</strong> Media*<br />
Plating Density<br />
Passage after<br />
Thawing<br />
Marker<br />
Population<br />
Doublings<br />
Page<br />
Human Pulmonary<br />
Microvascular<br />
Endothelial <strong>Cell</strong>s (HPMEC)<br />
Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s (HUtMEC)<br />
C-12281 C-22020<br />
C-22022<br />
C-12295 C-22020<br />
C-22022<br />
10,000 - 20,000<br />
cells per cm²<br />
10,000 - 20,000<br />
cells per cm²<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
P2 vWF +<br />
CD31 +<br />
Dil-Ac-LDL uptake +<br />
> 15 19<br />
> 15 19<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human Brain Microvascular<br />
Endothelial <strong>Cell</strong>s (HBMEC)<br />
C-12287 10,000 - 20,000<br />
cells per cm²<br />
> 10 20<br />
P2 vWF +<br />
Dil-Ac-LDL uptake +<br />
CD31 +<br />
Human Nasal<br />
Epithelial <strong>Cell</strong>s (HNEpC)<br />
C-12620 C-21060 10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 21<br />
Human Tracheal<br />
Epithelial <strong>Cell</strong>s (HTEpC)<br />
C-12644 C-21060 10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 21<br />
Human Bronchial<br />
Epithelial <strong>Cell</strong>s (HBEpC)<br />
C-12640 C-21060 10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 22<br />
Human Small Airway<br />
Epithelial <strong>Cell</strong>s (HSAEpC)<br />
C-12642 C-21070 10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 22<br />
Human Mammary<br />
Epithelial <strong>Cell</strong>s (HMEpC)<br />
C-12650 C-21010 10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 23<br />
Human Renal<br />
Epithelial <strong>Cell</strong>s (HREpC)<br />
C-12665 C-26001<br />
C-26030<br />
10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 23<br />
Human Renal Cortical<br />
Epithelial <strong>Cell</strong>s (HRCEpC)<br />
C-12660 C-26001<br />
C-26030<br />
10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 15 24<br />
Human Placental<br />
Epithelial <strong>Cell</strong>s (HPlEpC)<br />
C-12395 C-26040 10,000 - 15,000<br />
cells per cm²<br />
P2 Cytokeratin + > 5 24<br />
Normal Human Dermal<br />
Fibroblasts (NHDF)<br />
juvenile foreskin<br />
Normal Human Dermal<br />
Fibroblasts (NHDF)<br />
adult donor<br />
Human Pulmonary<br />
Fibroblasts (HPF)<br />
C-12300 C-23010 3,500 - 7,000<br />
cells per cm²<br />
C-12302 C-23020 3,500 - 7,000<br />
cells per cm²<br />
C-12360 C-23020 3,500 - 7,000<br />
cells per cm²<br />
P2 CD90 + > 15 25<br />
P2 CD90 + > 15 25<br />
P2 CD90 + > 15 26<br />
Human Aortic Adventitial<br />
Fibroblasts (HAoAF)<br />
C-12380 C-23020 3,500 - 7,000<br />
cells per cm²<br />
P2 CD90 + > 10 26<br />
Human Cardiac<br />
Fibroblasts (HCF)<br />
C-12375 C-23025 3,500 - 7,000<br />
cells per cm²<br />
P2 CD90 + > 15 26<br />
Human Uterine<br />
Fibroblasts (HUF)<br />
C-12385 C-23020 3,500 - 7,000<br />
cells per cm²<br />
P2 CD90 + > 15 27<br />
Human Villous Mesenchymal<br />
Fibroblasts (HVMF)<br />
C-12390 C-23020 3,500 - 7,000<br />
cells per cm²<br />
P2 CD90 + > 15 27<br />
Human Follicle Dermal<br />
Papilla <strong>Cell</strong>s (HFDPC)<br />
C-12071 C-26501 5,000 - 10,000<br />
cells per cm²<br />
P2<br />
Alkaline<br />
> 10 28<br />
phosphatase +<br />
Human Hepatocytes (HHpC) C-12850 C-25010<br />
C-25020<br />
* The media in this table are ready-to-use. For Media Kits see chapter 4.<br />
150,000<br />
cells per cm²<br />
n/a 29<br />
P0 Phase I reaction +<br />
Albumin +<br />
Phase II reaction +
42<br />
1 Human Primary <strong>Cell</strong>s<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Product<br />
Normal Human Epidermal<br />
Keratinocytes (NHEK)<br />
juvenile foreskin, single donor<br />
Normal Human Epidermal<br />
Keratinocytes (NHEK)<br />
juvenile foreskin, pooled<br />
Normal Human Epidermal<br />
Keratinocytes (NHEK)<br />
adult donor, single donor<br />
Normal Human Epidermal<br />
Keratinocytes (NHEK)<br />
adult donor, pooled<br />
Normal Human Epidermal<br />
Melanocytes (NHEM)<br />
juvenile foreskin<br />
Normal Human Epidermal<br />
Melanocytes (NHEM)<br />
juvenile foreskin,<br />
cultured in M2 Medium<br />
Normal Human Epidermal<br />
Melanocytes (NHEM)<br />
adult donor,<br />
cultured in M2 Medium<br />
C-12001 C-20011 5,000<br />
cells per cm²<br />
C-12005 C-20011 5,000<br />
cells per cm²<br />
C-12003 C-20011 5,000<br />
cells per cm²<br />
C-12006 C-20011 5,000<br />
cells per cm²<br />
C-12400 C-24010 5,000 - 10,000<br />
cells per cm²<br />
C-12402 C-24300 5,000 - 10,000<br />
cells per cm²<br />
C-12403 C-24300 5,000 - 10,000<br />
cells per cm²<br />
Human Osteoblasts (HOB) C-12720 C-27001<br />
C-27020<br />
Human White Preadipocytes<br />
(HWP) subcutaneous<br />
Human White Preadipocytes<br />
(HWP) visceral<br />
Human Skeletal Muscle <strong>Cell</strong>s<br />
(SkMC)<br />
Human Aortic<br />
Smooth Muscle <strong>Cell</strong>s<br />
(HAoSMC)<br />
Human Coronary Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
(HCASMC)<br />
Human Pulmonary Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
(HPASMC)<br />
Human Umbilical Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
(HUASMC)<br />
Human Tracheal<br />
Smooth Muscle <strong>Cell</strong>s (HTSMC)<br />
Human Bronchial<br />
Smooth Muscle <strong>Cell</strong>s (HBSMC)<br />
Human Bladder<br />
Smooth Muscle <strong>Cell</strong>s<br />
(HBdSMC)<br />
Human Prostate<br />
Smooth Muscle <strong>Cell</strong>s (HPrSMC)<br />
Human Uterine<br />
Smooth Muscle <strong>Cell</strong>s<br />
(HUtSMC)<br />
Cat. No.<br />
Cryopr. <strong>Cell</strong>s<br />
Recommended<br />
<strong>Culture</strong> Media*<br />
C-12730 C-27410<br />
C-27436<br />
C-27438<br />
C-12732 C-27410<br />
C-27436<br />
C-27438<br />
C-12530 C-23060<br />
C-23061<br />
Plating Density<br />
10,000 - 20,000<br />
cells per cm²<br />
5,000<br />
cells per cm²<br />
5,000<br />
cells per cm²<br />
3,500 - 7,000<br />
cells per cm²<br />
C-12533 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12511 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12521 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12500 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12565 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12561 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12571 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12573 C-22062 7,500 - 10,000<br />
cells per cm²<br />
C-12575 C-22062 7,500 - 10,000<br />
cells per cm²<br />
Passage after<br />
Thawing<br />
P2 Cytokeratin + > 15 30<br />
P2 Cytokeratin + > 15 30<br />
P2 Cytokeratin + > 15 30<br />
P2 Cytokeratin + > 15 30<br />
P2 Mel-5 + > 15 31<br />
P2 Mel-5 + > 15 31<br />
P2 Mel-5 + > 15 31<br />
P2<br />
P2<br />
P2<br />
Alkaline<br />
phosphatase +<br />
Mineralization tested<br />
Differentiation<br />
tested<br />
Differentiation<br />
tested<br />
> 10 32<br />
> 10 33<br />
> 10 33<br />
P2 Sarcomeric myosin +<br />
Differentiation<br />
tested<br />
> 15 34<br />
P2 Smooth muscle > 15 35<br />
α-actin +<br />
P2<br />
P2<br />
P2<br />
P2<br />
P2<br />
P2<br />
P2<br />
P2<br />
Marker<br />
Population<br />
Doublings<br />
Page<br />
Smooth muscle > 15 36<br />
α-actin +<br />
Smooth muscle > 15 36<br />
α-actin +<br />
Smooth muscle > 15 37<br />
α-actin +<br />
Smooth muscle<br />
α-actin + > 15 37<br />
Smooth muscle > 15 38<br />
α-actin +<br />
Smooth muscle > 15 38<br />
α-actin +<br />
Smooth muscle<br />
α-actin + > 15 39<br />
Smooth muscle > 15 39<br />
α-actin +<br />
* The media in this table are ready-to-use. For Media Kits see chapter 4.
1 Human Primary <strong>Cell</strong>s 43<br />
Human Primary <strong>Cell</strong>s by Tissue Type<br />
Product Page Product Page<br />
• Adipose Tissue<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Adipose Tissue (hMSC-AT)<br />
47<br />
• Brain<br />
Human Brain Microvascular <strong>Cell</strong>s (HBMEC)<br />
20<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
Human White Preadipocytes (HWP)<br />
subcutaneous<br />
Human White Preadipocytes (HWP)<br />
visceral<br />
• Bladder<br />
33<br />
33<br />
• Heart<br />
Human Cardiac Fibroblasts (HCF) 26<br />
Human Cardiac Myocytes (HCM) 9<br />
Human Bladder Microvascular<br />
Endothelial <strong>Cell</strong>s (HBdMEC)<br />
18<br />
Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s (HCMEC)<br />
18<br />
Human Bladder<br />
Smooth Muscle <strong>Cell</strong>s (HBdSMC<br />
38<br />
• Kidney<br />
• Blood/Bone Marrow<br />
Human Renal Epithelial <strong>Cell</strong>s (HREpC) 23<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Bone Marrow (hMSC-BM)<br />
47<br />
Human Renal Cortical Epithelial <strong>Cell</strong>s<br />
(HRCEpC)<br />
24<br />
Human Mononuclear <strong>Cell</strong>s from Peripheral<br />
Blood (hMNC-PB) single donor, ultra-pure<br />
Human Mononuclear <strong>Cell</strong>s from Peripheral<br />
Blood (hMNC-PB) pooled, ultra-pure<br />
Human Mononuclear <strong>Cell</strong>s from Cord<br />
Blood (hMNC-CB) single donor, ultra-pure<br />
Human Mononuclear <strong>Cell</strong>s from<br />
Cord Blood (hMNC-CB) pooled, ultra-pure<br />
Human CD34 + Progenitor <strong>Cell</strong>s from<br />
Cord Blood (hCD34 + -CB) single donor<br />
Human CD34 + Progenitor <strong>Cell</strong>s from<br />
Cord Blood (hCD34 + -CB) pooled<br />
Human CD133 + Progenitor <strong>Cell</strong>s from<br />
Cord Blood (hCD133 + -CB) single donor<br />
Human CD133 + Progenitor <strong>Cell</strong>s from<br />
Cord Blood (hCD133 + -CB) pooled<br />
Human CD14 + Monocytes from Peripheral<br />
Blood (hMoCD14 + -PB), single donor<br />
Human CD14 + Monocytes from Peripheral<br />
Blood (hMoCD14 + -PB), single donor,<br />
pre-screened<br />
Human Monocytes from Peripheral Blood<br />
(hMo-PB), single donor<br />
Human Monocytes from Peripheral Blood<br />
(hMo-PB), single donor, pre-screened<br />
• Bone/Joint/Skeletal System<br />
54<br />
54<br />
54<br />
54<br />
50<br />
50<br />
51<br />
51<br />
52<br />
52<br />
52<br />
52<br />
• Large Vessels<br />
Human Aortic Adventitial<br />
26<br />
Fibroblasts (HAoAF)<br />
Human Aortic Endothelial <strong>Cell</strong>s (HAoEC) 13<br />
Human Aortic<br />
Smooth Muscle <strong>Cell</strong>s (HAoSMC)<br />
Human Coronary Artery<br />
Endothelial <strong>Cell</strong>s (HCAEC)<br />
Human Coronary Artery<br />
Smooth Muscle <strong>Cell</strong>s (HCASMC)<br />
Human Pulmonary Artery<br />
Endothelial <strong>Cell</strong>s (HPAEC)<br />
Human Pulmonary Artery<br />
Smooth Muscle <strong>Cell</strong>s (HPASMC)<br />
Human Umbilical Artery<br />
Endothelial <strong>Cell</strong>s (HUAEC)<br />
Human Umbilical Artery<br />
Smooth Muscle <strong>Cell</strong>s (HUASMC)<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC) single donor<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC) pooled<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC) pre-screened<br />
35<br />
13<br />
36<br />
14<br />
36<br />
12<br />
37<br />
11<br />
11<br />
11<br />
Human Osteoblasts (HOB) 32<br />
Human Saphenous Vein<br />
Endothelial <strong>Cell</strong>s (HSaVEC)<br />
14<br />
Human Chondrocytes (HCH) 10<br />
Human Skeletal Muscle <strong>Cell</strong>s (SkMC) 34
44<br />
1 Human Primary <strong>Cell</strong>s<br />
Product Page Product Page<br />
1 Human Primary<br />
<strong>Cell</strong>s<br />
• Liver<br />
Human Hepatocytes (HHpC) 29<br />
• Respiratory System<br />
Human Nasal Epithelial <strong>Cell</strong>s (HNEpC) 21<br />
• Mammary Tissue<br />
Human Tracheal Epithelial <strong>Cell</strong>s (HTEpC) 21<br />
Human Mammary<br />
Epithelial <strong>Cell</strong>s (HMEpC)<br />
23<br />
Human Tracheal<br />
Smooth Muscle <strong>Cell</strong>s (HTSMC)<br />
37<br />
• Umbilical Cord<br />
Human Umbilical Artery<br />
Endothelial <strong>Cell</strong>s (HUAEC)<br />
Human Umbilical Artery<br />
Smooth Muscle <strong>Cell</strong>s (HUASMC)<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC) single donor<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC) pooled<br />
Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s (HUVEC) pre-screened<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Umbilical Cord Matrix (hMSC-UC)<br />
• Uterus<br />
Human Uterine Fibroblasts (HUF) 27<br />
Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s (HUtMEC)<br />
Human Uterine<br />
Smooth Muscle <strong>Cell</strong>s (HUtSMC)<br />
• Placenta<br />
Human Pericytes from Placenta (hPC-PL) 49<br />
Human Placental Epithelial <strong>Cell</strong>s (HPlEpC) 24<br />
Human Villous Mesenchymal<br />
Fibroblasts (HVMF)<br />
• Prostate<br />
Human Prostate<br />
Smooth Muscle <strong>Cell</strong>s (HPrSMC)<br />
12<br />
37<br />
11<br />
11<br />
11<br />
47<br />
19<br />
39<br />
27<br />
39<br />
• Skin<br />
Human Bronchial Epithelial <strong>Cell</strong>s (HBEpC) 22<br />
Human Bronchial<br />
Smooth Muscle <strong>Cell</strong>s (HBSMC)<br />
Human Small Airway<br />
Epithelial <strong>Cell</strong>s (HSAEpC)<br />
Human Pulmonary Fibroblasts (HPF) 26<br />
Human Pulmonary Microvascular<br />
Endothelial <strong>Cell</strong>s (HPMEC)<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC) juvenile foreskin<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC) adult donor<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (HDMEC) pre-screened<br />
Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s (HDBEC) juvenile foreskin<br />
Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s (HDBEC) adult donor<br />
Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s (HDLEC) juvenile foreskin<br />
Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s (HDLEC) adult donor<br />
Normal Human Dermal<br />
Fibroblasts (NHDF) juvenile foreskin<br />
Normal Human Dermal<br />
Fibroblasts (NHDF) adult donor<br />
Human Follicle Dermal<br />
Papilla <strong>Cell</strong>s (HFDPC)<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) juvenile foreskin, single donor<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) juvenile foreskin, pooled<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) adult, single donor<br />
Normal Human Epidermal Keratinocytes<br />
(NHEK) adult, pooled<br />
38<br />
22<br />
19<br />
15<br />
15<br />
15<br />
16<br />
16<br />
17<br />
17<br />
25<br />
25<br />
28<br />
30<br />
30<br />
30<br />
30<br />
Normal Human Epidermal<br />
Melanocytes (NHEM) juvenile foreskin<br />
Normal Human Epidermal Melanocytes (NHEM)<br />
juvenile foreskin, cultured in M2 Medium<br />
Normal Human Epidermal Melanocytes (NHEM)<br />
adult donor, cultured in M2 Medium<br />
31<br />
31<br />
31
Human Stem and Blood <strong>Cell</strong>s<br />
2<br />
Promo<strong>Cell</strong> offers a collection of adult stem cells, as well as<br />
differentiated blood cells from normal human bone marrow,<br />
umbilical cord tissue, placenta, adipose tissue, peripheral blood,<br />
and cord blood. All cells are obtained from approved donor programs<br />
at associated medical centers. High-quality media that<br />
simulate the in vivo environment as well as optimized differentiation<br />
media systems are also available for these cell types.
46<br />
Content Chapter 2<br />
2<br />
Human Stem and Blood <strong>Cell</strong>s<br />
2.1 Mesenchymal Stem <strong>Cell</strong>s ............. 47<br />
2.2 Pericytes .......................... 49<br />
2.3 CD34 + Progenitor <strong>Cell</strong>s ............... 50<br />
2.4 CD133 + Progenitor <strong>Cell</strong>s .............. 51<br />
2.5<br />
new<br />
Monocytes ...................... 52<br />
2.6 Mononuclear <strong>Cell</strong>s new ................ 54<br />
Overview Human Stem and Blood <strong>Cell</strong>s. ....... 56
2 Human Stem and Blood <strong>Cell</strong>s 47<br />
2.1 Mesenchymal Stem <strong>Cell</strong>s<br />
Applications<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Bone Marrow (hMSC-BM)<br />
Recommended media & reagents<br />
Mesenchymal Stem <strong>Cell</strong><br />
Stem cell research<br />
Growth Medium (see page 86)<br />
Regenerative medicine research<br />
Promo<strong>Cell</strong> hMSC-BM are harvested from<br />
Mesenchymal Stem <strong>Cell</strong><br />
Tissue engineering studies<br />
normal human bone marrow. The cells<br />
Growth Medium DXF (see page 86)<br />
Developmental experiments<br />
are tested for their ability to differentiate<br />
Mesenchymal Stem <strong>Cell</strong> Adipogenic<br />
Features<br />
in vitro into adipocytes, chondrocytes,<br />
osteoblasts, and neuronal cells.<br />
Differentiation Medium (see page 86)<br />
Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium (see page 86)<br />
Multipotent adult stem cells<br />
Tested for in vitro differentiation<br />
Routinely analyzed by flow cytometry<br />
Growth Medium and five optimized<br />
Differentiation Media available<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Umbilical Cord Matrix<br />
(hMSC-UC)<br />
Promo<strong>Cell</strong> hMSC-UC are harvested from<br />
Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium w/o Inducers<br />
(see page 86)<br />
Mesenchymal Stem <strong>Cell</strong> Osteogenic<br />
Differentiation Medium (see page 86)<br />
Mesenchymal Stem <strong>Cell</strong> Neurogenic<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Mesenchymal Stem <strong>Cell</strong>s (MSC), also<br />
normal human umbilical cord matrix<br />
Differentiation Medium (see page 86)<br />
termed Mesenchymal Stromal <strong>Cell</strong>s, are<br />
(Wharton’s jelly). The cells are tested for<br />
DetachKit (see page 101)<br />
multipotent cells that can differentiate<br />
their ability to differentiate in vitro into<br />
<strong>Cell</strong> Dissociation Solution ACF<br />
into a variety of cell types and have the<br />
adipocytes, chondrocytes, osteoblasts,<br />
(see page 102)<br />
capacity for self renewal. MSC have<br />
and neuronal cells.<br />
Fibronectin Solution, human<br />
been shown to differentiate in vitro or<br />
(see page 105)<br />
in vivo into adipocytes, chondrocytes,<br />
osteoblasts, myocytes, neurons, hepatocytes,<br />
and pancreatic islet cells. Opti-<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Adipose Tissue (hMSC-AT)<br />
Related products<br />
MSC-Qualified Fetal Calf Serum<br />
mized Promo<strong>Cell</strong> media are available to<br />
(see page 93)<br />
support both the growth of MSC and<br />
Promo<strong>Cell</strong> hMSC-AT are harvested from<br />
hMSC-BM Pellet (see page 98)<br />
their differentiation into several differ-<br />
normal human adipose tissue. The cells<br />
hMSC-UC Pellet (see page 98)<br />
ent lineages. Recent experiments sug-<br />
are tested for their ability to differentiate<br />
hMSC-AT Pellet (see page 98)<br />
gest that differentiation capabilities into<br />
in vitro into adipocytes, chondrocytes,<br />
diverse cell types vary between MSC of<br />
osteoblasts, and neuronal cells.<br />
different origin.<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
Fig. 1: Undifferentiated hMSC-BM cul-<br />
Fig. 2: Undifferentiated hMSC-BM cul-<br />
tured in MSC Growth Medium.<br />
tured in MSC Growth Medium DXF.
48<br />
2 Human Stem and Blood <strong>Cell</strong>s<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Fig. 3.1 Fig. 3.2<br />
Fig. 3.1: hMSC-BM after in vitro differentiation<br />
into adipocytes using Promo<strong>Cell</strong><br />
Mesenchymal Stem <strong>Cell</strong> Adipogenic<br />
Differentiation Medium (lipid droplets<br />
stained with Oil Red O).<br />
Fig. 3.2: hMSC-BM spheroid after in vitro<br />
differentiation into cartilage using Promo<strong>Cell</strong><br />
Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium (Alcian Blue<br />
staining).<br />
Fig. 3.3: hMSC-BM after in vitro differentiation<br />
into osteoblasts using Promo<strong>Cell</strong><br />
Mesenchymal Stem<strong>Cell</strong> Osteogenic Differentiation<br />
Medium (Alizarin Red S staining).<br />
Fig. 3.4: hMSC-BM after in vitro differentiation<br />
into neuronal cells using Promo<strong>Cell</strong><br />
Mesenchymal Stem <strong>Cell</strong> Neurogenic Differentiation<br />
Medium.<br />
Fig. 3.3 Fig. 3.4<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Bone Marrow (hMSC-BM)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12974<br />
C-12975<br />
hMSC-BM Pellet 1x10 6 cells in RNAlater ® C-14090<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Umbilical Cord Matrix (hMSC-UC)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12971<br />
C-12972<br />
hMSC-UC Pellet 1x10 6 cells in RNAlater ® C-14091<br />
Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Adipose Tissue (hMSC-AT)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12977<br />
C-12978<br />
hMSC-AT Pellet 1x10 6 cells in RNAlater ® C-14092
2 Human Stem and Blood <strong>Cell</strong>s 49<br />
2.2 Pericytes<br />
Applications<br />
blood vessel walls. They are important<br />
for angiogenesis, the structural integ-<br />
Angiogenesis research<br />
rity of the microvasculature, and blood<br />
Regenerative medicine research<br />
flow regulation. However, they can also<br />
Oncology<br />
develop into malignant tumors called<br />
hemangiopericytomas.<br />
Features<br />
To date, pericytes have demonstrated<br />
Non-hematopoietic multipotent<br />
the ability to differentiate into adipo-<br />
mesenchymal-like cells<br />
Important in angiogenesis<br />
Routinely characterized by flow<br />
cytometry<br />
Isolated from human placenta<br />
cytes, osteoblasts, fibroblasts, smooth<br />
muscle cells, and phagocytes (macrophages).<br />
Recommended media & reagents<br />
Pericyte Growth Medium<br />
Fig. 4: Proliferating human pericytes<br />
(hPC-PL) from the placenta (phase contrast).<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Pericytes are multipotent mesenchymallike<br />
cells found in association with small<br />
(see page 87)<br />
DetachKit (see page 101)<br />
Related products<br />
hPC-PL Pellet (see page 98)<br />
Count<br />
Fig. 5: Flow cytometric analysis of Promo<strong>Cell</strong> Pericytes.<br />
> 98% of the cells are CD146 + .<br />
50<br />
25<br />
0<br />
CD146 – CD146 +<br />
10 0 10 1 10 2 10 3 10 4<br />
CD146<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human Pericytes from Placenta (hPC-PL)<br />
500,000 cryopreserved cells<br />
500,000 proliferating cells<br />
C-12980<br />
C-12981<br />
hPC-PL Pellet 1x10 6 cells in RNAlater ® C-14095
50<br />
2 Human Stem and Blood <strong>Cell</strong>s<br />
2.3 CD34 + Progenitor <strong>Cell</strong>s<br />
Applications<br />
marker for primitive blood- and bone<br />
Recommended media & reagents<br />
marrow-derived progenitor cells, espe-<br />
Hematopoietic Progenitor <strong>Cell</strong><br />
Stem cell research<br />
cially for hematopoietic and endothelial<br />
Expansion Medium DXF<br />
Transplantation basic research<br />
stem cells. Although the biological func-<br />
(see page 87)<br />
Hematological and immunological<br />
tions of CD34 are largely unknown, re-<br />
Hematopoietic Progenitor Medium<br />
studies<br />
cent data suggest that CD34 is involved<br />
(see page 87)<br />
Vascular and angiogenesis research<br />
in maintenance of the progenitor cells in<br />
Endothelial Progenitor Medium<br />
Oncology research<br />
a phenotypically undifferentiated state.<br />
(see page 88)<br />
Promo<strong>Cell</strong> offers CD34 + Progenitor <strong>Cell</strong>s<br />
Features<br />
from the cord blood of healthy donors.<br />
Related products<br />
The CD34 + Progenitor <strong>Cell</strong>s contain<br />
G-CSF (see page 106)<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Multipotent hematopoietic/<br />
endothelial progenitors<br />
CD34 + cell purity > 95%<br />
Superior viability<br />
two main cellular subpopulations, hematopoietic<br />
and endothelial progenitor<br />
cells. Therefore, CD34 + Progenitor <strong>Cell</strong>s<br />
are suitable for a series of studies, e.g.<br />
SCF (see page 106)<br />
flt-3 ligand (see page 106)<br />
IL-3 CC (see page 106)<br />
IL-6 CC (see page 106)<br />
directed differentiation into more com-<br />
TPO (see page 106)<br />
CD34 is a glycosylated transmembrane<br />
mitted types of blood cells and endothe-<br />
CD34 antibody<br />
protein and represents a well-known<br />
lial lineages.<br />
(see www.promokine.info)<br />
Count<br />
Fig. 6: Flow cytometric analysis of Promo<strong>Cell</strong> CD34 +<br />
Progenitor <strong>Cell</strong>s. > 95% of the cells are CD34 + .<br />
50<br />
25<br />
0<br />
0<br />
CD34 – CD34 +<br />
10 10 2 10 3 10 4<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
CD34<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human CD34 + Progenitor <strong>Cell</strong>s from Cord<br />
Blood (hCD34 + -CB), single donor<br />
Human CD34 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood (hCD34 + -CB), pooled<br />
100,000 cryopreserved cells C-12921<br />
100,000 cryopreserved cells C-12924
2 Human Stem and Blood <strong>Cell</strong>s 51<br />
2.4 CD133 + Progenitor <strong>Cell</strong>s<br />
Applications<br />
CD133 (AC133, Prominin-1) is a glyco-<br />
Recommended media & reagents<br />
protein with sub-cellular localization in<br />
Hematopoietic Progenitor <strong>Cell</strong><br />
Hematology research<br />
plasma membrane protrusions. In hu-<br />
Expansion Medium DXF (see page 87)<br />
Stem cell research<br />
mans, this protein was originally identi-<br />
Cytokine Mix E for HPC Expansion<br />
Oncology<br />
fied as an antigenic marker expressed<br />
Medium DXF (see page 87)<br />
Features<br />
on hematopoietic stem cells. In addition,<br />
CD133 is also expressed by neural and<br />
Hematopoietic Progenitor Medium<br />
(see page 87)<br />
muscle progenitor cells.<br />
Primitive hematopoietic progenitors<br />
Related products<br />
Available from cord blood<br />
CD133 + cells are isolated from fresh cord<br />
G-CSF (see page 106)<br />
CD133 + cell purity > 95%<br />
blood. These cells are highly prolifera-<br />
SCF (see page 106)<br />
Superior viability<br />
Expansion and Differentiation Media<br />
available<br />
tive primitive hematopoietic progenitors.<br />
They can give rise to mature terminally<br />
differentiated hematopoietic cells<br />
by means of directed differentiation.<br />
flt-3 ligand (see page 106)<br />
IL-3 CC (see page 106)<br />
IL-6 CC (see page 106)<br />
TPO (see page 106)<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Promo<strong>Cell</strong> also provides suitable expan-<br />
CD133 antibody<br />
sion and differentiation media.<br />
(see www.promokine.info)<br />
CD45<br />
Fig. 7: Flow cytometric analysis of cord blood-derived<br />
CD133 + Progenitor <strong>Cell</strong>s, demonstrating their high<br />
purity.<br />
10 3<br />
10 2<br />
10<br />
0<br />
CD133 + cells<br />
0 10 10 2 10 3<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
CD133<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human CD133 + Progenitor <strong>Cell</strong>s from<br />
Cord Blood (hCD133 + -CB), single donor<br />
Human CD133 + Progenitor <strong>Cell</strong>s from<br />
Cord Blood (hCD133 + -CB), pooled<br />
100,000 cryopreserved cells C-12927<br />
100,000 cryopreserved cells C-12929
52<br />
2 Human Stem and Blood <strong>Cell</strong>s<br />
2.5 Monocytes<br />
Applications<br />
gradient-based method completely<br />
Recommended media & reagents<br />
devoid of using any immunomagnetic<br />
Mononuclear <strong>Cell</strong> Medium (see page 89)<br />
Dendritic <strong>Cell</strong> Research<br />
beads. The advantage of these com-<br />
DC Generation Medium (see page 88)<br />
(Auto-)Immunity<br />
pletely native Monocytes is that poten-<br />
Infection biology<br />
tial effects of bound magnetic particles<br />
Related products<br />
Features<br />
(which can be ingested by this phagocytic<br />
cell type) are excluded.<br />
hMoCD14 + -PB single donor Pellet<br />
(see page 98)<br />
hMoCD14 + -PB single donor<br />
Isolated from fresh peripheral blood<br />
Routinely analyzed by flow cytometry<br />
CD14 + -selected as well as “untouched“<br />
Human CD14 + Monocytes<br />
(hMoCD14 + -PB)<br />
pre-screened Pellet (see page 98)<br />
hMo-PB single donor Pellet<br />
(see page 98)<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Monocytes available<br />
Monocytes (Mo) are immature phagocytic<br />
cells circulating in the blood. Acting<br />
Promo<strong>Cell</strong> Human CD14 + Monocytes<br />
are isolated from fresh peripheral blood.<br />
After isolation of the ultra-pure mono-<br />
hMo-PB single donor<br />
pre-screened Pellet (see page 98)<br />
PromoFectin-Macrophage<br />
Transfection Reagent<br />
as antigen-presenting immune cells, they<br />
nuclear cell fraction by proprietary meth-<br />
(see PromoKine catalog)<br />
can phagocytize and degrade microbes<br />
ods, the CD14 + Monocytes are isolated<br />
G-CSF (see page 106)<br />
and particulate matter. Monocytes can<br />
by positive selection with > 95% purity.<br />
GM-CSF (see page 106)<br />
differentiate into several different cell<br />
IL-4 CC (see page 106)<br />
types. The most common applications<br />
In addition, hMoCD14 + -PB are available<br />
TNF-alpha (see page 106)<br />
for Monocytes are the in vitro differen-<br />
which are pre-screened for their differ-<br />
M-CSF (see page 106)<br />
tiation into monocyte-derived dendritic<br />
entiation capacity into dendritic cells.<br />
sRANKL (see page 106)<br />
cells (moDC), macrophages, and osteo-<br />
CD14 antibody<br />
clasts as well as their use as phagocytic<br />
immune cells in immunology and infec-<br />
Human Monocytes (hMo-PB)<br />
(see www.promokine.info)<br />
tion biology.<br />
Promo<strong>Cell</strong> Human “untouched” Mono-<br />
CD14 belongs to the family of LPS re-<br />
cytes are isolated from fresh peripheral<br />
ceptor antigens and is strongly expressed<br />
blood. After isolation of the ultra-pure<br />
on the majority of Monocytes. Thus,<br />
mononuclear cell fraction by proprietary<br />
binding of immunomagnetic particles<br />
methods, the Monocyte fraction is fur-<br />
to this antigen is employed for purifica-<br />
ther purified to > 70% by an advanced<br />
tion of Promo<strong>Cell</strong> CD14 + -Monocytes<br />
multi-gradient technique.<br />
(hMoCD14 + -PB) from Mononuclear<br />
<strong>Cell</strong>s (MNC).<br />
In contrast, the “untouched” Monocytes<br />
(hMo-PB) provided by Promo<strong>Cell</strong><br />
In addition, hMo-PB are available which<br />
are pre-screened for their differentiation<br />
capacity into dendritic cells.<br />
Fig. 8: Cluster formation during<br />
differentiation of Monocytes into moDC.<br />
are enriched from MNC by a density<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
Fig. 9: Mature moDC cultured in<br />
Promo<strong>Cell</strong> DC Generation Medium showing<br />
typical dendrite-like structures.
2 Human Stem and Blood <strong>Cell</strong>s 53<br />
Count<br />
Fig. 10a: Flow cytometric analysis of day 8 mature<br />
moDCs generated in the Promo<strong>Cell</strong> DC Generation<br />
Medium. More than 98% of the cells are negative for<br />
CD14.<br />
12,5<br />
0<br />
0<br />
CD14 -<br />
CD14 +<br />
10 1 10 2 10 3 10 4<br />
CD14<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Count<br />
Fig. 10b: Flow cytometric analysis of day 8 mature<br />
moDCs generated in the Promo<strong>Cell</strong> DC Generation<br />
Medium. The DC maturation marker CD83 is strongly<br />
expressed by > 98% of the cells.<br />
12,5<br />
0<br />
0<br />
CD83 -<br />
CD83 +<br />
10 1 10 2 10 3 10 4<br />
CD83<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Human CD14 + Monocytes from Peripheral<br />
Blood (hMoCD14 + -PB), single donor<br />
10 x 10 6 new<br />
cryopreserved cells C-12909<br />
hMoCD14 + -PB single donor Pellet 1x10 6 cells in RNAlater ® new C-14110<br />
Human CD14 + Monocytes from Peripheral<br />
Blood (hMoCD14 + -PB), single donor,<br />
pre-screened<br />
hMoCD14 + -PB single donor<br />
pre-screened Pellet<br />
10 x 10 6 new<br />
cryopreserved cells C-12912<br />
1x10 6 cells in RNAlater ® new<br />
C-14112<br />
Human Monocytes from Peripheral Blood<br />
10 x 10 6 cryopreserved cells<br />
new<br />
C-12910<br />
(hMo-PB), single donor<br />
hMo-PB single donor Pellet 1x10 6 cells in RNAlater ® new C-14111<br />
Human Monocytes from Peripheral Blood<br />
10 x 10 6 cryopreserved cells<br />
new<br />
C-12913<br />
(hMo-PB), single donor, pre-screened<br />
hMo-PB single donor pre-screened Pellet 1x10 6 cells in RNAlater ® new<br />
C-14113
54<br />
2 Human Stem and Blood <strong>Cell</strong>s<br />
2.6 Mononuclear <strong>Cell</strong>s<br />
Applications<br />
Promo<strong>Cell</strong> Mononuclear <strong>Cell</strong>s (MNC)<br />
represent the lymphocyte and monocyte<br />
tors, monocytes, and lymphocyte subpopulations<br />
(see. Fig. 12).<br />
fraction isolated from umbilical cord blood<br />
Stem cell research<br />
Transplantation basic research<br />
or peripheral blood. They are prepared<br />
by optimized low-density gradient cen-<br />
Human Mononuclear <strong>Cell</strong>s from<br />
Peripheral Blood (hMNC-PB)<br />
Hematological and immunological<br />
trifugation, which effectively reduces the<br />
studies<br />
granulocyte content (see Fig. 11). Red<br />
Adult peripheral blood contains large<br />
Oncology research<br />
blood cells are gently but thoroughly de-<br />
numbers of mature immune cells but also<br />
Virology and infection biology<br />
pleted by proprietary techniques instead<br />
some progenitor cells. Typical application<br />
Features<br />
of osmotic lysis. This results in ultra-pure<br />
mononuclear cells with superior viability<br />
fields are immunology, oncology, hematology,<br />
virology, and infection biology.<br />
and unchanged biological function. In<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Routinely analyzed by flow cytometry<br />
Granulocyte content < 5%<br />
Red blood cells depleted without lysis<br />
High purity and viability<br />
addition, the cells do not clump after<br />
thawing, allowing for easy handling and<br />
reproducible results.<br />
The MNC can be used directly for ex-<br />
Human Mononuclear <strong>Cell</strong>s from<br />
Cord Blood (hMNC-CB)<br />
hMNC-CB are isolated from umbilical<br />
Available from single and pooled<br />
periments or as a starting material for<br />
cord blood, which has a much higher<br />
donors<br />
the isolation of several cell types, e.g.,<br />
content of primitive progenitor cells com-<br />
hematopoietic and endothelial progeni-<br />
pared to adult peripheral blood.<br />
Size [µm]<br />
Granulocytes<br />
Monocytes<br />
Fig. 11: Flow cytometric analysis of Mononuclear<br />
<strong>Cell</strong>s from Cord Blood (hMNC-CB). Granulocyte<br />
content 1%.<br />
16<br />
14<br />
12<br />
10<br />
8<br />
0<br />
Lymphocytes<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
0 10 10 2 10 3<br />
10 4<br />
Side Scatter<br />
CD45<br />
10 4<br />
CD34 + cells<br />
Fig. 12: Flow cytometric determination of CD34 + cells<br />
within the Promo<strong>Cell</strong> Mononuclear <strong>Cell</strong>s from Cord<br />
Blood (hMNC-CB).<br />
10 3<br />
10 2<br />
10<br />
0<br />
<strong>Cell</strong> Lab Quanta SC TM , Beckman Coulter, Inc.<br />
0 10 10 2 10 3 10 4<br />
CD34
2 Human Stem and Blood <strong>Cell</strong>s 55<br />
Recommended media & reagents<br />
Mononuclear <strong>Cell</strong> Medium<br />
(see page 89)<br />
Hematopoietic Progenitor Medium*<br />
(see page 87)<br />
Endothelial Progenitor Medium*<br />
(see page 88)<br />
Hematopoietic Progenitor <strong>Cell</strong><br />
Expansion Medium DXF (see page 87)<br />
Cytokine Mix E for HPC Expansion<br />
Medium DXF (see page 87)<br />
Related products<br />
hMNC-PB single donor Pellet<br />
(see page 97)<br />
hMNC-CB single donor Pellet<br />
(see page 97)<br />
hMNC-PB pooled Pellet<br />
(see page 97)<br />
hMNC-CB pooled Pellet<br />
(see page 97)<br />
Lymphocyte Separation<br />
Medium 1077 (see page 89)<br />
GM-CSF (see page 106)<br />
IL-4 CC (see page 106)<br />
M-CSF (see page 106)<br />
TNF-alpha (see page 106)<br />
sRANKL (see page 106)<br />
https://www.promocell.com/shop<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Product Size Catalog Number<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood (hMNC-PB),<br />
single donor, ultra-pure<br />
25 x 10 6 cryopreserved cells C-12907<br />
hMNC-PB single donor Pellet 1x10 6 cells in RNAlater ® C-14098<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood (hMNC-PB),<br />
pooled, ultra-pure<br />
25 x 10 6 new<br />
cryopreserved cells C-12908<br />
hMNC-PB pooled Pellet 1x10 6 cells in RNAlater ® new<br />
C-14099<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood (hMNC-CB),<br />
single donor, ultra-pure<br />
25 x 10 6 cryopreserved cells C-12901<br />
hMNC-CB single donor Pellet 1x10 6 cells in RNAlater ® C-14096<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood (hMNC-CB),<br />
pooled, ultra-pure<br />
25 x 10 6 cryopreserved cells C-12904<br />
hMNC-CB pooled Pellet 1x10 6 cells in RNAlater ® C-14097<br />
* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or “Hill-assays” for endothelial progenitor cells<br />
(Hill et al. 2003, N Engl J Med 348: 593-600).
56<br />
2 Human Stem and Blood <strong>Cell</strong>s<br />
Overview Human Stem and Blood <strong>Cell</strong>s<br />
Product<br />
Cat. No.<br />
Cryopr.<br />
<strong>Cell</strong>s<br />
Recommended<br />
<strong>Culture</strong> Media<br />
Recommended<br />
Differentiation<br />
Media<br />
Recommended<br />
Sera<br />
Plating<br />
Density<br />
Page<br />
Human Mesenchymal Stem<br />
<strong>Cell</strong>s from Bone Marrow<br />
(hMSC-BM)<br />
C-12974 C-28010<br />
C-28019<br />
C-28011<br />
C-28012<br />
C-28013<br />
C-28014<br />
C-28015<br />
C-37385<br />
C-37386<br />
4,000 cells 47<br />
per cm 2<br />
2 Human Stem<br />
and Blood <strong>Cell</strong>s<br />
Human Mesenchymal Stem<br />
<strong>Cell</strong>s from Umbilical Cord<br />
Matrix (hMSC-UC)<br />
Human Mesenchymal Stem<br />
<strong>Cell</strong>s from Adipose Tissue<br />
(hMSC-AT)<br />
C-12971 C-28010<br />
C-28019<br />
C-12977 C-28010<br />
C-28019<br />
C-28011<br />
C-28012<br />
C-28013<br />
C-28014<br />
C-28015<br />
C-28011<br />
C-28012<br />
C-28013<br />
C-28014<br />
C-28015<br />
C-37385<br />
C-37386<br />
C-37385<br />
C-37386<br />
4,000 cells 47<br />
per cm 2<br />
4,000 cells 47<br />
per cm 2<br />
Human Pericytes<br />
from Placenta (hPC-PL)<br />
C-12980 C-28040 C-37385<br />
C-37386<br />
4,000 cells 49<br />
per cm 2<br />
Human CD34 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood (hCD34 + -CB),<br />
single donor<br />
C-12921 C-28021<br />
C-39890<br />
C-28020*<br />
C-28025*<br />
C-37387<br />
C-37388<br />
5,000 cells<br />
per ml<br />
50<br />
Human CD34 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood (hCD34 + -CB),<br />
pooled<br />
C-12924 C-28021<br />
C-39890<br />
C-28020*<br />
C-28025*<br />
C-37387<br />
C-37388<br />
5,000 cells<br />
per ml<br />
50<br />
Human CD133 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood (hCD133 + -CB),<br />
single donor<br />
C-12927 C-28021<br />
C-39890<br />
C-28020* C-37387<br />
C-37388<br />
5,000 cells<br />
per ml<br />
51<br />
Human CD133 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood (hCD133 + -<br />
CB), pooled<br />
C-12929 C-28021<br />
C-39890<br />
C-28020* C-37387<br />
C-37388<br />
5,000 cells<br />
per ml<br />
51<br />
Human CD14 + Monocytes<br />
from Peripheral Blood<br />
(hMoCD14 + -PB), single donor<br />
C-12909 C-28030 C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
52<br />
Human CD14 + Monocytes<br />
from Peripheral Blood<br />
(hMoCD14 + -PB), single donor,<br />
pre-screened<br />
C-12912 C-28030 C-28050 C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
52<br />
Human Monocytes from<br />
Peripheral Blood (hMo-PB),<br />
single donor<br />
C-12910 C-28030 C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
52<br />
Human Monocytes from<br />
Peripheral Blood (hMo-PB),<br />
pre-screened<br />
C-12913 C-28030 C-28050 C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
52<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood (hMNC-<br />
PB), single donor, ultra-pure<br />
C-12907 C-28030 C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
54<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood (hMNC-<br />
PB), pooled, ultra-pure<br />
C-12908 C-28030 C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
54<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood (hMNC-CB),<br />
single donor, ultra-pure<br />
C-12901 C-28030<br />
C-28021<br />
C-39890<br />
C-28020*<br />
C-28025*<br />
C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
54<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood (hMNC-CB),<br />
pooled, ultra-pure<br />
C-12904 C-28030<br />
C-28021<br />
C-39890<br />
C-28020*<br />
C-28025*<br />
C-37387<br />
C-37388<br />
1 x10 6 cells<br />
per ml<br />
54<br />
* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or “Hill-assays” for endothelial<br />
progenitor cells (Hill et al. 2003, N Engl J Med 348: 593-600).
<strong>Cell</strong> Model Systems<br />
3<br />
Promo<strong>Cell</strong> offers a set of ready-to-use <strong>Cell</strong> Model Systems,<br />
which include primary cells and optimized media. In vivo<br />
situations are very closely mimicked by these model systems<br />
because of the spatial arrangement of the cultured cells, and<br />
they allow easy monitoring of complex cell growth processes<br />
and fast screening of test substances.
58<br />
Content Chapter 3<br />
3<br />
<strong>Cell</strong> Model Systems<br />
3.1 Angiogenesis Assays and Kits .......... 59
3 <strong>Cell</strong> Model Systems 59<br />
3.1 Angiogenesis Assays and Kits<br />
Applications<br />
controlled by pro-angiogenic and antiangiogenic<br />
factors:<br />
Angiogenesis research<br />
Atherosclerosis research<br />
Proteolytic activity<br />
Inflammation studies<br />
Angiogenic growth factors activate<br />
Oncology investigations<br />
receptors on endothelial cells in pre-<br />
Chemokine/Chemotaxis assays<br />
existing blood vessels. The activated en-<br />
Drug screening<br />
dothelial cells begin to release proteases<br />
that degrade the basement membrane.<br />
Features<br />
Migration<br />
Four different assays available<br />
Endothelial cells from the parent vessel<br />
Available with HUVEC or HDMEC<br />
walls migrate through the gaps in the<br />
Fig. 1: Vessel network with newly<br />
Ready to use<br />
basement membrane into the surround-<br />
formed capillaries.<br />
Fast results<br />
ing tissue.<br />
Easy monitoring<br />
Proliferation<br />
Promo<strong>Cell</strong> offers four diverse angiogen-<br />
Angiogenesis, the growth of new blood<br />
The migrated endothelial cells proliferate<br />
esis models to simulate these angiogen-<br />
vessels from pre-existing ones, is a normal<br />
process in growth and development,<br />
as well as in wound healing. However,<br />
it is also vitally involved in a number of<br />
and form solid sprouts. These sprouts<br />
extend toward the source of the angiogenic<br />
stimulus.<br />
esis stages or the whole process in vitro,<br />
thus making pro- or anti-angiogenic<br />
testing fast and efficient:<br />
3 <strong>Cell</strong> Model<br />
Systems<br />
pathological processes like tumor growth<br />
Lumen formation<br />
3D-Angiogenesis Assay<br />
and metastasis, rheumatoid arthritis, and<br />
The cells in the solid sprouts form a lu-<br />
Planar Migration Assay<br />
macular degeneration. It occurs in four<br />
men, which results in a fully developed<br />
Transmigration Kit<br />
well-characterized stages, which are<br />
vessel.<br />
Invasion Kit<br />
3D-Angiogenesis Assay<br />
within two days and can be quantified<br />
without cell staining (see Fig. 2).<br />
The Promo<strong>Cell</strong> ready-to-use 3D-An-<br />
The number and length of the sprouts<br />
giogenesis Assay allows fast and easy<br />
correspond to the pro- or anti-angioge-<br />
screening for pro-angiogenic and anti-<br />
nicity of the test substance.<br />
angiogenic effects of test substances in<br />
Since only one cell type is used in the as-<br />
a convenient 24-well format.<br />
say, unwanted side effects of co-cultured<br />
The assay simulates the entire angiogenesis<br />
process in vitro - proteolytic<br />
cells are excluded.<br />
100 μM<br />
activity, migration, proliferation, and lu-<br />
Substances that have already been<br />
men formation - all in three dimensions.<br />
analyzed using the 3D-Angiogene-<br />
Therefore, the migration of tip cells<br />
sis Assay: VEGF (see Fig. 2 and 3),<br />
or the formation of three dimensional<br />
FGF-1, FGF-2, and kinase inhibitors<br />
networks can also be monitored. This is<br />
e.g. SU5402, SU5614 (see Fig. 4),<br />
clearly an advantage over other angio-<br />
SU6656.<br />
genesis assays like the tube formation<br />
assay.<br />
The assay utilizes endothelial cell<br />
spheroids embedded in a collagen ma-<br />
100 μM<br />
trix, which very closely resembles the in<br />
Fig. 2: Endothelial cell spheroids 48<br />
vivo basement membrane environment.<br />
hours after stimulation with 20 ng/ml<br />
After successful stimulation, new blood<br />
VEGF* (top) and unstimulated negative<br />
vessels sprout into the collagen matrix<br />
control (bottom).<br />
* VEGF ist provided as a positive control with the Assay.
60<br />
3 <strong>Cell</strong> Model Systems<br />
Cumulative sprout length [μm]<br />
1400<br />
1200<br />
1000<br />
800<br />
600<br />
400<br />
Fig. 3: Rising VEGF concentrations lead to increased<br />
cumulative sprout length.<br />
200<br />
0<br />
0<br />
0.78 1.56 3.12 6.25 12.5 25<br />
VEGF [ng/ml]<br />
3 <strong>Cell</strong> Model<br />
Systems<br />
Cumulative sprout length [μm]<br />
1400<br />
1200<br />
1000<br />
Fig. 4: Increasing concentrations of the kinase inhibitor<br />
SU5614 added to VEGF [25 ng/ml] stimulated spheroids.<br />
Rising SU5614 concentrations lead to decreased<br />
cumulative sprout length (Control: no VEGF and no<br />
SU5614 added).<br />
800<br />
600<br />
400<br />
200<br />
0<br />
0 1 10 10 2 10 3 10 4 control<br />
SU5614 [nM]<br />
Advantages of the Promo<strong>Cell</strong><br />
3D-Angiogenesis Assay:<br />
3D growth of new blood vessels<br />
Results available in 2 days<br />
No staining necessary for monitoring<br />
Automated analysis possible<br />
Related products<br />
Human Umbilical Vein Endothelial<br />
<strong>Cell</strong>s pre-screened (see page 11)<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s pre-screened<br />
(see page 15)<br />
FGF-1, FGF-2 (see page 106)<br />
SU5402 (see www.promokine.info)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
3D-Angiogenesis Assay (HUVEC)<br />
3D-Angiogenesis Assay (HDMEC)<br />
8 assays<br />
2 x 8 assays<br />
3 x 8 assays<br />
8 assays<br />
2 x 8 assays<br />
3 x 8 assays<br />
C-13010<br />
C-13011<br />
C-13012<br />
C-13020<br />
C-13021<br />
C-13022<br />
Note: The assays will always be delivered on Wednesdays - please start immediately after arrival.
3 <strong>Cell</strong> Model Systems 61<br />
Planar Migration Assay<br />
The Promo<strong>Cell</strong> Planar Migration Assay<br />
allows fast and easy screening for migration<br />
stimulating effects of test substances<br />
in vitro. <strong>Cell</strong>s from a confluent<br />
area migrate into a cell free section at a<br />
speed which is influenced by the applied<br />
test substance. After 3-4 days, the distance<br />
between the two migration fronts<br />
is measured (cell staining is not necessary).<br />
This distance is proportional to the<br />
migration stimulating capacity of the applied<br />
test substance (see Fig. 5).<br />
The cell free section is created at<br />
Promo<strong>Cell</strong> by a special culture technique<br />
and not by the scraping of cells. This ensures<br />
undamaged cells at the edge and a<br />
defined migration front.<br />
The assay is ready-to-use and consists of<br />
primary endothelial cells with a defined<br />
migration front and optimized medium.<br />
Substances that have already been analyzed<br />
using the Migration Assay:<br />
VEGF, FGF-1, FGF-2, kinase inhibitors<br />
e.g. SU5614.<br />
Related products<br />
VEGF (see page 106)<br />
FGF-1, FGF-2 (see page 106)<br />
2000 µm 1500 µm<br />
Fig. 5: Promo<strong>Cell</strong> Planar Migration<br />
Assay without VEGF (left) and with<br />
25 ng/ml VEGF in the medium (right).<br />
3 <strong>Cell</strong> Model<br />
Systems<br />
Distance between migration fronts [μm]<br />
2000<br />
1500<br />
1000<br />
Fig. 6: Comparison of endothelial cell migration with<br />
and without VEGF using the Promo<strong>Cell</strong> Planar Migration<br />
Assay.<br />
500<br />
0<br />
0 25<br />
VEGF [ng/ml]<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Planar Migration Assay (HUVEC) 12 assays C-13030<br />
Planar Migration Assay (HDMEC) 12 assays C-13031<br />
Note: The assays will always be delivered on Wednesdays - please start immediately after arrival.
62<br />
3 <strong>Cell</strong> Model Systems<br />
Transmigration Kit<br />
The Promo<strong>Cell</strong> Transmigration Kit utilizes<br />
the migration of endothelial cells<br />
along a cytokine gradient (chemotaxis)<br />
to measure the chemotactic capability of<br />
test substances in vitro. The basis for the<br />
kit are two compartments separated by<br />
a membrane with an accurately defined<br />
pore size of 8 μm (see Fig. 7). A potential<br />
chemotactic factor is placed in one compartment<br />
and a gradient develops across<br />
the membrane. Endothelial cells placed<br />
in the other compartment migrate along<br />
this gradient. Within four hours after<br />
adding the test substance, the number<br />
of cells that have migrated through the<br />
membrane can easily be counted (see<br />
Fig. 8). This number is proportional to<br />
the chemotactic capability of the tested<br />
substance. The kit is ready-to-use and<br />
consists of primary endothelial cells (proliferating),<br />
test chambers, and optimized<br />
medium.<br />
Substances that have already been analyzed<br />
using the Transmigration Kit:<br />
VEGF, FGF-1, FGF-2, kinase inhibitors<br />
e.g. SU5614.<br />
Related products<br />
VEGF (see page 106)<br />
FGF-1, FGF-2 (see page 106)<br />
1. 2. 3. 4.<br />
3 <strong>Cell</strong> Model<br />
Systems<br />
Fig. 7: Workflow of the Promo<strong>Cell</strong> Transmigration Kit.<br />
1. Primary cells are added to the cell culture insert and the test substance is added into the lower compartment.<br />
2. The cells attach to the membrane of the insert.<br />
3. The cells migrate into the lower compartment through the pores of the membrane.<br />
4. After migration, the cells attach to the lower surface of the membrane and can be analyzed.<br />
Fig. 8: <strong>Cell</strong>s that have migrated<br />
through the membrane without VEGF<br />
(left) and with 20 ng/ml VEGF in the<br />
medium (right).<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Transmigration Kit (HUVEC) 24 assays C-13035<br />
Transmigration Kit (HDMEC) 24 assays C-13036<br />
Note: The kits will always be delivered on Wednesdays - please start immediately after arrival.
3 <strong>Cell</strong> Model Systems 63<br />
Invasion Kit<br />
The Promo<strong>Cell</strong> Invasion Kit simulates the<br />
proteolytic activity of endothelial cells<br />
and their directed migration in vitro. In<br />
this fashion, it is possible to analyze the<br />
influence of different substances on the<br />
proteolytic activity and their chemotactic<br />
efficacy in one assay.<br />
The basis for the kit are two compartments<br />
separated by a membrane with<br />
an accurately defined pore size of 8 μm<br />
(see Fig. 9). This membrane is covered<br />
by a matrix, which mimics the basement<br />
membrane of blood vessels. A potential<br />
chemotactic factor is placed in one<br />
compartment and a gradient develops<br />
across the membrane. Endothelial cells<br />
placed in the other compartment degrade<br />
the matrix and then migrate along<br />
this gradient. Within 24 hours after adding<br />
the test substance, the number of<br />
cells, which have migrated through the<br />
membrane, can easily be counted. This<br />
number is proportional to the chemotactic<br />
capability of the tested substance and<br />
its influence on the proteolytic activity.<br />
The kit is ready-to-use and consists of<br />
primary endothelial cells (proliferating),<br />
coated test chambers, and optimized<br />
medium.<br />
Substances that have already been analyzed<br />
using the Invasion Kit:<br />
VEGF, FGF-1, FGF-2, kinase inhibitors<br />
e.g. SU5614.<br />
Related products<br />
VEGF (see page 106)<br />
FGF-1, FGF-2 (see page 106)<br />
1. 2. 3. 4. 5.<br />
3 <strong>Cell</strong> Model<br />
Systems<br />
Fig. 9: Workflow of the Promo<strong>Cell</strong> Invasion Kit.<br />
1. Primary cells are added to the cell culture insert and the test substance is added to the lower compartment.<br />
2. The cells attach to the extracellular matrix on the membrane of the insert.<br />
3. The cells release proteases that degrade the basement membrane.<br />
4. The cells migrate into the lower compartment through the pores of the membrane.<br />
5. After migration, the cells attach to the lower surface of the membrane and can be analyzed.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Invasion Kit (HUVEC) 24 assays C-13040<br />
Invasion Kit (HDMEC) 24 assays C-13041<br />
Note: The kits will always be delivered on Wednesdays - please start immediately after arrival.
Media and Sera<br />
4<br />
High-quality media and sera are vital for successful cell culture.<br />
Therefore, Promo<strong>Cell</strong> adheres to strict quality standards and<br />
produces media and sera only from raw materials from strictly<br />
approved sources. We also have invested a lot of effort in media<br />
optimization and have substituted serum by defined substances<br />
wherever possible. This ensures effective cell growth<br />
and avoids the unpredictable effects of indeterminate compounds.
66<br />
Content Chapter 4<br />
4<br />
Media and Sera<br />
4.1 Media for Primary <strong>Cell</strong>s .............. 67<br />
4.1.1 Myocyte Medium. .......................68<br />
4.1.2 Chondrocyte Medium ....................68<br />
4.1.3 Endothelial <strong>Cell</strong> Media ....................69<br />
4.1.4 Endothelial <strong>Cell</strong> Media MV. ................70<br />
4.1.5 Epithelial <strong>Cell</strong> Media. .....................71<br />
4.1.6 Fibroblast Media new ......................76<br />
4.1.7 Follicle Dermal Papilla <strong>Cell</strong> Medium ..........78<br />
4.1.8 Hepatocyte Media .......................79<br />
4.1.9 Keratinocyte Medium. ....................80<br />
4.1.10 Melanocyte Media .......................81<br />
4.1.11 Osteoblast Media. .......................82<br />
4.1.12 Preadipocyte/Adipocyte Media .............83<br />
4.1.13 Skeletal Muscle <strong>Cell</strong> Media. ................84<br />
4.1.14 Smooth Muscle <strong>Cell</strong> Medium ...............85<br />
4.1.15 Freezing Medium ........................85<br />
4.2 Media for Stem and Blood <strong>Cell</strong>s. ........ 86<br />
4.2.1 Mesenchymal Stem <strong>Cell</strong> Media new ...........86<br />
4.2.2 Pericyte Medium ........................87<br />
4.2.3 Hematopoietic Progenitor <strong>Cell</strong> Media. ........87<br />
4.2.4 Endothelial Progenitor <strong>Cell</strong> Medium ..........88<br />
4.2.5 Dendritic <strong>Cell</strong> Generation Media new ..........88<br />
4.2.6 Mononuclear <strong>Cell</strong> Medium. ................89<br />
4.2.7 Blood <strong>Cell</strong> Separation Medium ..............89<br />
4.3 Classical Media ..................... 90<br />
4.4 Insect <strong>Cell</strong> <strong>Culture</strong> Media ............. 92<br />
4.5 Sera ............................. 92
4 Media and Sera 67<br />
4.1 Media for Primary <strong>Cell</strong>s<br />
The Promo<strong>Cell</strong> media for primary cells<br />
are formulated for the optimal growth<br />
of specific types of primary human cells<br />
and a variety of primary animal cells.<br />
For most cell types, they are available<br />
as Medium (Ready-to-use) for maximal<br />
convenience or as Medium Kit, which<br />
allows user control over the supplement<br />
type and concentration.<br />
Each medium lot is tested for its ability to<br />
support the growth of the intended primary<br />
cells. In addition, biochemical and<br />
sterility tests are performed*.<br />
Note: The media for primary cells do not<br />
contain antibiotics.<br />
Medium (Ready-to-use)<br />
The Medium (Ready-to-use) consists of<br />
a 500 ml bottle with Basal Medium and<br />
one vial with SupplementMix. Adding<br />
the SupplementMix to the Basal Medium<br />
results in the complete medium, which<br />
contains all the growth factors and<br />
supplements necessary for the propagation<br />
of the specific human primary cells<br />
in culture (including L-Glutamine and<br />
NaHCO 3<br />
).<br />
The shelf life of the Basal Medium is<br />
9 months (at 4 to 8°C), that of the<br />
SupplementMix is 12 months (at -20°C)<br />
from the date of manufacture. After<br />
adding the SupplementMix, the shelf life<br />
of the complete medium is 6 weeks (at<br />
4 to 8°C).<br />
Medium Kit<br />
The Medium Kit consists of a 500 ml<br />
bottle with Basal Medium and a SupplementPack.<br />
The SupplementPack is a set<br />
of individual vials with pre-measured<br />
supplements. Adding all components of<br />
the SupplementPack to the Basal Medium<br />
results in the complete medium,<br />
which contains all the growth factors<br />
and supplements necessary for the propagation<br />
of specific human primary cells<br />
in culture (including L-Glutamine and<br />
NaHCO 3<br />
). Since all supplements can be<br />
added separately, the user has full control<br />
over the media formulation and can<br />
adapt it to his/her experimental requirements.<br />
The shelf life of the Basal Medium is<br />
9 months (at 4 to 8°C), and that of<br />
the SupplementPack is 12 months<br />
(at -20°C) from the date of manufacture.<br />
After adding the supplements,<br />
the shelf life of the complete<br />
medium is 6 weeks (at 4 to 8°C).<br />
4 Media and Sera<br />
Basal Media (with or without phenol<br />
red) as well as SupplementMixes and<br />
SupplementPacks can also be purchased<br />
separately.<br />
If you require special media modifications, we offer a Custom Media Service starting at 10 bottles per order.<br />
Please ask for details.<br />
*Certificates of analysis are available on request.
68<br />
4 Media and Sera<br />
Low Serum<br />
4.1.1 Myocyte Medium<br />
Myocyte Growth Medium<br />
Recommended for<br />
Human Cardiac Myocytes<br />
(see page 9)<br />
The Promo<strong>Cell</strong> Myocyte Growth Medium<br />
is a low serum (5% V/V) medium supplemented<br />
with well chosen hormones and<br />
growth factors. It was developed for the<br />
optimal expansion and maintenance of<br />
primary cardiac myocytes in culture. The<br />
medium is optimized for primary human<br />
cells but can also be used for primary porcine<br />
cardiac myocytes.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
0.05 ml / ml<br />
0.5 ng / ml<br />
2 ng / ml<br />
5 µg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Myocyte Growth Medium (Ready-to-use) 500 ml C-22070<br />
4 Media and Sera<br />
Myocyte Growth Medium Kit 500 ml C-22170<br />
Myocyte Basal Medium 500 ml C-22270<br />
Myocyte Basal Medium, phenol red-free 500 ml C-22275<br />
Myocyte Growth Medium SupplementMix for 500 ml C-39275<br />
Myocyte Growth Medium<br />
for 500 ml C-39270<br />
SupplementPack<br />
4.1.2 Chondrocyte Medium<br />
Chondrocyte Growth Medium<br />
Recommended for<br />
Human Chondrocytes (see page 10)<br />
The Promo<strong>Cell</strong> Chondrocyte Growth<br />
Medium is designed for the optimal<br />
cultivation of human chondrocytes. The<br />
fetal calf serum in the medium is strictly<br />
pretested for optimal performance.<br />
The medium is optimized for primary<br />
human cells but can also be used for primary<br />
porcine and rat chondrocytes.<br />
Final supplement concentration<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
0.1 ml / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Chondrocyte Growth Medium<br />
(Ready-to-use)<br />
500 ml C-27101<br />
Chondrocyte Basal Medium 500 ml C-27111<br />
Chondrocyte Basal Medium,<br />
phenol red-free<br />
Chondrocyte Growth Medium<br />
SupplementMix<br />
500 ml C-27115<br />
for 500 ml C-39635
4 Media and Sera 69<br />
Low Serum<br />
4.1.3 Endothelial <strong>Cell</strong> Media<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
Recommended for<br />
Human Umbilical Vein Endothelial<br />
<strong>Cell</strong>s (see page 11)<br />
Human Umbilical Artery Endothelial<br />
<strong>Cell</strong>s (see page 12)<br />
Human Pulmonary Artery Endothelial<br />
<strong>Cell</strong>s (see page 14)<br />
Human Saphenous Vein Endothelial<br />
<strong>Cell</strong>s (see page 14)<br />
Endothelial <strong>Cell</strong> Growth Medium and<br />
Endothelial <strong>Cell</strong> Growth Medium 2 are<br />
low-serum (2% V/V) media optimized<br />
for the cultivation of endothelial cells<br />
from large blood vessels.<br />
Endothelial <strong>Cell</strong> Growth Medium 2 lacks<br />
Endothelial <strong>Cell</strong> Growth Supplement<br />
(ECGS, bovine hypothalamic extract)<br />
but contains Insulin-like Growth Factor<br />
(Long R3 IGF) and Vascular Endothelial<br />
Growth Factor (VEGF). Generally, VEGF<br />
leads to higher endothelial cell proliferation<br />
in culture. But, because of its multiple<br />
effects on cell metabolism, it may<br />
also interfere with certain experimental<br />
setups. In these cases, we recommend<br />
the use of Endothelial <strong>Cell</strong> Growth Medium,<br />
which contains no VEGF.<br />
The media are optimized for primary<br />
human cells but can also be used for<br />
bovine, porcine, murine, caprine, rabbit,<br />
elephant, dog, and rat endothelial cells<br />
from large vessels as well as for various<br />
endothelial cell lines.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Endothelial <strong>Cell</strong> Growth Medium Endothelial <strong>Cell</strong> Growth Medium 2<br />
Fetal Calf Serum 0.02 ml / ml 0.02 ml / ml<br />
Endothelial <strong>Cell</strong> Growth Supplement 0.004 ml / ml —<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
0.1 ng / ml 5 ng / ml<br />
Basic Fibroblast Growth Factor<br />
1 ng / ml 10 ng / ml<br />
(recombinant human)<br />
Insulin-like Growth Factor (Long R3 IGF) — 20 ng / ml<br />
Vascular Endothelial Growth Factor 165<br />
— 0.5 ng / ml<br />
(recombinant human)<br />
Ascorbic Acid — 1 µg / ml<br />
Heparin 90 μg / ml 22.5 µg / ml<br />
Hydrocortisone 1 µg / ml 0.2 µg / ml<br />
4 Media and Sera<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
(Ready-to-use)<br />
500 ml C-22010<br />
Endothelial <strong>Cell</strong> Growth Medium Kit 500 ml C-22110<br />
Endothelial <strong>Cell</strong> Basal Medium 500 ml C-22210<br />
Endothelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
SupplementMix<br />
Endothelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
(Ready-to-use)<br />
500 ml C-22215<br />
for 500 ml C-39215<br />
for 500 ml C-39210<br />
500 ml C-22011<br />
Endothelial <strong>Cell</strong> Growth Medium 2 Kit 500 ml C-22111<br />
Endothelial <strong>Cell</strong> Basal Medium 2 500 ml C-22211<br />
Endothelial <strong>Cell</strong> Basal Medium 2,<br />
phenol red-free<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
SupplementMix<br />
Endothelial <strong>Cell</strong> Growth Medium 2<br />
SupplementPack<br />
500 ml C-22216<br />
for 500 ml C-39216<br />
for 500 ml C-39211
70<br />
4 Media and Sera<br />
Low Serum<br />
4.1.4 Endothelial <strong>Cell</strong> Media MV<br />
Endothelial <strong>Cell</strong> Growth<br />
Medium MV<br />
Endothelial <strong>Cell</strong> Growth<br />
Medium MV2<br />
Recommended for<br />
Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 15)<br />
Human Coronary Artery Endothelial<br />
<strong>Cell</strong>s (see page 13)<br />
Human Aortic Endothelial <strong>Cell</strong>s<br />
(see page 13)<br />
Human Pulmonary Microvascular<br />
Endothelial <strong>Cell</strong>s (see page 19)<br />
Human Dermal Lymphatic Endothelial<br />
<strong>Cell</strong>s (see page 17)<br />
Human Dermal Blood Endothelial<br />
<strong>Cell</strong>s (see page 16)<br />
Promo<strong>Cell</strong> Endothelial <strong>Cell</strong> Growth Medium<br />
MV and Endothelial <strong>Cell</strong> Growth<br />
Medium MV2 were developed for the<br />
in vitro cultivation of endothelial cells<br />
from microvascular vessels, the coronary<br />
artery, and the aorta.<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
lacks the Endothelial <strong>Cell</strong> Growth Supplement<br />
(ECGS, bovine hypothalamic<br />
extract) but contains the basic Fibroblast<br />
Growth Factor, the Insulin-like Growth<br />
Factor (Long R3 IGF-1) and the Vascular Endothelial<br />
Growth Factor (VEGF). Generally,<br />
VEGF leads to higher endothelial cell proliferation<br />
in culture. But, because of its multiple<br />
effects on the cell metabolism, it may also<br />
interfere with certain experimental setups.<br />
In these cases, we recommend the use of<br />
Endothelial <strong>Cell</strong> Growth Medium MV.<br />
The media are optimized for primary<br />
human cells but can also be used for<br />
bovine, porcine, murine, and rat endothelial<br />
cells from microvascular vessels as<br />
well as for various endothelial cell lines.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium) Endothelial <strong>Cell</strong> Growth Medium MV Endothelial <strong>Cell</strong> Growth Medium MV2<br />
Fetal Calf Serum 0.05 ml / ml 0.05 ml / ml<br />
4 Media and Sera<br />
Endothelial <strong>Cell</strong> Growth Supplement 0.004 ml / ml —<br />
Epidermal Growth Factor<br />
10 ng / ml 5 ng / ml<br />
(recombinant human)<br />
Basic Fibroblast Growth Factor<br />
— 10 ng / ml<br />
(recombinant human)<br />
Insulin-like Growth Factor (Long R3 IGF-1) — 20 ng / ml<br />
Vascular Endothelial Growth Factor 165<br />
— 0.5 ng / ml<br />
(recombinant human)<br />
Ascorbic Acid — 1 µg / ml<br />
Heparin 90 μg / ml —<br />
Hydrocortisone 1 µg / ml 0.2 µg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
(Ready-to-use)<br />
500 ml C-22020<br />
Endothelial <strong>Cell</strong> Growth Medium MV Kit 500 ml C-22120<br />
Endothelial <strong>Cell</strong> Basal Medium MV 500 ml C-22220<br />
Endothelial <strong>Cell</strong> Basal Medium MV,<br />
phenol red-free<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
SupplementMix<br />
Endothelial <strong>Cell</strong> Growth Medium MV<br />
SupplementPack<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
(Ready-to-use)<br />
500 ml C-22225<br />
for 500 ml C-39225<br />
for 500 ml C-39220<br />
500 ml C-22022<br />
Endothelial <strong>Cell</strong> Growth Medium MV2 Kit 500 ml C-22121<br />
Endothelial <strong>Cell</strong> Basal Medium MV2 500 ml C-22221<br />
Endothelial <strong>Cell</strong> Basal Medium MV2,<br />
phenol red-free<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
SupplementMix<br />
Endothelial <strong>Cell</strong> Growth Medium MV2<br />
SupplementPack<br />
500 ml C-22226<br />
for 500 ml C-39226<br />
for 500 ml C-39221
4 Media and Sera 71<br />
Serum-Free<br />
4.1.5 Epithelial <strong>Cell</strong> Media<br />
Airway Epithelial <strong>Cell</strong> Growth<br />
Medium<br />
Recommended for<br />
Human Nasal Epithelial <strong>Cell</strong>s<br />
(see page 21)<br />
Human Bronchial Epithelial <strong>Cell</strong>s<br />
(see page 22)<br />
Human Tracheal Epithelial <strong>Cell</strong>s<br />
(see page 21)<br />
The Promo<strong>Cell</strong> Airway Epithelial <strong>Cell</strong><br />
Growth Medium is a serum-free medium<br />
optimized for the cultivation of epithelial<br />
cells from large air passages.<br />
The medium is optimized for primary<br />
human cells but can also be used for<br />
murine, porcine, equine, and rat airway<br />
epithelial cells.<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Bovine Pituitary Extract<br />
0.004 ml / ml<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
10 ng / ml<br />
Insulin (recombinant human)<br />
5 μg / ml<br />
Hydrocortisone<br />
0.5 μg / ml<br />
Epinephrine<br />
0.5 μg / ml<br />
Triiodo-L-thyronine<br />
Transferrin, holo (human)<br />
Retinoic Acid<br />
6.7 ng / ml<br />
10 μg / ml<br />
0.1 ng / ml<br />
https://www.promocell.com/shop<br />
4 Media and Sera<br />
Product Size Catalog Number<br />
Airway Epithelial <strong>Cell</strong> Growth Medium<br />
(Ready-to-use)<br />
500 ml C-21060<br />
Airway Epithelial <strong>Cell</strong> Growth Medium Kit 500 ml C-21160<br />
Airway Epithelial <strong>Cell</strong> Basal Medium 500 ml C-21260<br />
Airway Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
Airway Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementMix<br />
Airway Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
500 ml C-21265<br />
for 500 ml C-39165<br />
for 500 ml C-39160
72<br />
4 Media and Sera<br />
Serum-Free<br />
Small Airway Epithelial <strong>Cell</strong><br />
Growth Medium<br />
Recommended for<br />
Human Small Airway Epithelial <strong>Cell</strong>s<br />
(see page 22)<br />
Promo<strong>Cell</strong> Small Airway Epithelial <strong>Cell</strong><br />
Growth Medium is a serum-free medium<br />
developed for the cultivation of epithelial<br />
cells from the distal respiratory system.<br />
The optimal adjustment of growth factors<br />
in this medium ensures excellent in vitro<br />
growth.<br />
The medium is optimized for primary human<br />
cells but can also be used for porcine<br />
small airway epithelial cells.<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Bovine Pituitary Extract<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
Hydrocortisone<br />
Epinephrine<br />
Triiodo-L-thyronine<br />
Transferrin, holo (human)<br />
Retinoic Acid<br />
Bovine Serum Albumin-Fatty Acid Free<br />
(BSA-FAF)<br />
0.004 ml / ml<br />
10 ng / ml<br />
5 μg / ml<br />
0.5 μg / ml<br />
0.5 μg / ml<br />
6.7 ng / ml<br />
10 μg / ml<br />
0.1 ng / ml<br />
2.5 mg / ml<br />
4 Media and Sera<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Small Airway Epithelial <strong>Cell</strong> Growth<br />
500 ml C-21070<br />
Medium (Ready-to-use)<br />
Small Airway Epithelial <strong>Cell</strong> Growth<br />
500 ml C-21170<br />
Medium Kit<br />
Small Airway Epithelial <strong>Cell</strong> Basal Medium 500 ml C-21270<br />
Small Airway Epithelial <strong>Cell</strong> Basal Medium,<br />
500 ml C-21275<br />
phenol red-free<br />
Small Airway Epithelial <strong>Cell</strong> Growth<br />
for 500 ml C-39175<br />
Medium SupplementMix<br />
Small Airway Epithelial <strong>Cell</strong> Growth<br />
Medium SupplementPack<br />
for 500 ml C-39170
4 Media and Sera 73<br />
Serum-Free<br />
Mammary Epithelial <strong>Cell</strong> Growth<br />
Medium<br />
Recommended for<br />
The Promo<strong>Cell</strong> Mammary Epithelial <strong>Cell</strong><br />
Growth Medium is a serum-free medium<br />
developed for the cultivation of epithelial<br />
cells from the mammary gland.<br />
The medium is optimized for primary human<br />
cells but can also be used for rat and<br />
murine mammary epithelial cells as well<br />
as mamma carcinoma cell lines.<br />
Human Mammary Epithelial <strong>Cell</strong>s<br />
(see page 23)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Bovine Pituitary Extract<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
Hydrocortisone<br />
0.004 ml / ml<br />
10 ng / ml<br />
5 μg / ml<br />
0.5 μg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Mammary Epithelial <strong>Cell</strong> Growth Medium<br />
(Ready-to-use)<br />
500 ml C-21010<br />
Mammary Epithelial <strong>Cell</strong> Growth Medium<br />
500 ml C-21110<br />
Kit<br />
Mammary Epithelial <strong>Cell</strong> Basal Medium 500 ml C-21210<br />
Mammary Epithelial <strong>Cell</strong> Basal Medium,<br />
500 ml C-21215<br />
phenol red-free<br />
Mammary Epithelial <strong>Cell</strong> Growth Medium<br />
for 500 ml C-39115<br />
SupplementMix<br />
Mammary Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
for 500 ml C-39110<br />
4 Media and Sera
74<br />
4 Media and Sera<br />
Serum-Free/Low Serum<br />
Renal Epithelial <strong>Cell</strong> Growth<br />
Medium<br />
Renal Epithelial <strong>Cell</strong> Growth<br />
Medium 2<br />
Recommended for<br />
Human Renal Epithelial <strong>Cell</strong>s<br />
(see page 23)<br />
Human Renal Cortical Epithelial <strong>Cell</strong>s<br />
(see page 24)<br />
The Promo<strong>Cell</strong> Renal Epithelial <strong>Cell</strong><br />
Growth Medium is a serum-free medium<br />
for the cultivation of epithelial cells from<br />
the kidneys.<br />
The Promo<strong>Cell</strong> Renal Epithelial <strong>Cell</strong><br />
Growth Medium 2 is an optimized lowserum<br />
(5% V/V) medium with epinephrine.<br />
The combination of low serum and<br />
selected growth factors ensures successful<br />
growth in vitro and makes this medium<br />
the first choice for renal epithelial cells.<br />
The media are optimized for primary human<br />
cells but can also be used for porcine<br />
renal epithelial cells.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium) Renal Epithelial <strong>Cell</strong> Growth Medium Renal Epithelial <strong>Cell</strong> Growth Medium 2<br />
Fetal Calf Serum — 0.05 ml / ml<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
10 ng / ml 10 ng / ml<br />
Insulin (recombinant human) 5 μg / ml 5 μg / ml<br />
Epinephrine — 0.5 µg / ml<br />
Hydrocortisone 36 ng / ml 36 ng / ml<br />
4 Media and Sera<br />
Transferrin, holo (human) 5 μg / ml 5 μg / ml<br />
Triiodo-L-thyronine 4 pg / ml 4 pg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Renal Epithelial <strong>Cell</strong> Growth Medium<br />
(Ready-to-use)<br />
500 ml C-26001<br />
Renal Epithelial <strong>Cell</strong> Growth Medium Kit 500 ml C-26011<br />
Renal Epithelial <strong>Cell</strong> Basal Medium 500 ml C-26021<br />
Renal Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
Renal Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementMix<br />
Renal Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
Renal Epithelial <strong>Cell</strong> Growth Medium 2<br />
(Ready-to-use)<br />
500 ml C-26022<br />
for 500 ml C-39604<br />
for 500 ml C-39600<br />
500 ml C-26030<br />
Renal Epithelial <strong>Cell</strong> Growth Medium 2 Kit 500 ml C-26130<br />
Renal Epithelial <strong>Cell</strong> Basal Medium 2 500 ml C-26230<br />
Renal Epithelial <strong>Cell</strong> Basal Medium 2,<br />
phenol red-free<br />
Renal Epithelial <strong>Cell</strong> Growth Medium 2<br />
SupplementMix<br />
Renal Epithelial <strong>Cell</strong> Growth Medium 2<br />
SupplementPack<br />
500 ml C-26235<br />
for 500 ml C-39606<br />
for 500 ml C-39605
4 Media and Sera 75<br />
Low Serum<br />
Placental Epithelial <strong>Cell</strong> Growth<br />
Medium<br />
Recommended for<br />
Human Placental Epithelial <strong>Cell</strong>s<br />
(see page 24)<br />
The Promo<strong>Cell</strong> Placental Epithelial <strong>Cell</strong><br />
Growth Medium is a low-serum (5%<br />
V/V) medium optimized for the cultivation<br />
of epithelial cells from the amniotic<br />
membrane of the placenta. The combination<br />
of low serum und selected growth<br />
factors ensures successful growth in vitro.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
Epinephrine<br />
Hydrocortisone<br />
Transferrin, holo (human)<br />
Triiodo-L-thyronine<br />
0.05 ml / ml<br />
10 ng / ml<br />
5 μg / ml<br />
0.5 µg / ml<br />
36 ng / ml<br />
5 μg / ml<br />
4 pg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Placental Epithelial <strong>Cell</strong> Growth Medium<br />
500 ml C-26040<br />
(Ready-to-use)<br />
Placental Epithelial <strong>Cell</strong> Growth<br />
500 ml C-26140<br />
Medium Kit<br />
Placental Epithelial <strong>Cell</strong> Basal Medium 500 ml C-26240<br />
Placental Epithelial <strong>Cell</strong> Basal Medium,<br />
500 ml C-26245<br />
phenol red-free<br />
Placental Epithelial <strong>Cell</strong> Growth Medium<br />
for 500 ml C-39612<br />
SupplementMix<br />
Placental Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
for 500 ml C-39610<br />
4 Media and Sera
76<br />
4 Media and Sera<br />
Serum-Free<br />
4.1.6 Fibroblast Media<br />
Fibroblast Growth Medium<br />
Recommended for<br />
Normal Human Dermal Fibroblasts,<br />
juvenile foreskin (see page 25)<br />
The Promo<strong>Cell</strong> Fibroblast Growth Medium<br />
is a serum-free medium optimized<br />
for the cultivation of neonatal and juvenile<br />
fibroblasts.<br />
The medium is optimized for primary human<br />
cells but can also be used for rat,<br />
porcine, and bovine fibroblasts as well as<br />
fibroblast cell lines.<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
1 ng / ml<br />
5 μg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Fibroblast Growth Medium (Ready-to-use) 500 ml C-23010<br />
Fibroblast Growth Medium Kit 500 ml C-23110<br />
Fibroblast Basal Medium 500 ml C-23210<br />
Fibroblast Basal Medium, phenol red-free 500 ml C-23215<br />
4 Media and Sera<br />
Fibroblast Growth Medium<br />
SupplementMix<br />
Fibroblast Growth Medium<br />
SupplementPack<br />
for 500 ml C-39315<br />
for 500 ml C-39310<br />
Low Serum<br />
Fibroblast Growth Medium 2<br />
Recommended for<br />
Normal Human Dermal Fibroblasts,<br />
adult donor (see page 25)<br />
Human Pulmonary Fibroblasts<br />
(see page 26)<br />
Human Aortic Adventitial Fibroblasts<br />
(see page 26)<br />
Human Uterine Fibroblasts<br />
(see page 27)<br />
Human Villious Mesenchymal<br />
Fibroblasts (see page 27)<br />
Promo<strong>Cell</strong> Fibroblast Growth Medium 2<br />
is a low-serum (2% V/V) medium developed<br />
for adult human fibroblasts.<br />
The medium is optimized for primary human<br />
cells but can also be used for rat,<br />
porcine, and bovine fibroblasts as well as<br />
fibroblast cell lines.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
0.02 ml / ml<br />
1 ng / ml<br />
5 μg / ml
4 Media and Sera 77<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Fibroblast Growth Medium 2<br />
(Ready-to-use)<br />
500 ml C-23020<br />
Fibroblast Growth Medium 2 Kit 500 ml C-23120<br />
Fibroblast Basal Medium 2 500 ml C-23220<br />
Fibroblast Basal Medium 2,<br />
phenol red-free<br />
Fibroblast Growth Medium 2<br />
SupplementMix<br />
Fibroblast Growth Medium 2<br />
SupplementPack<br />
500 ml C-23225<br />
for 500 ml C-39325<br />
for 500 ml C-39320<br />
Fibroblast Growth Medium 3<br />
Recommended for<br />
Human Cardiac Fibroblasts<br />
(see page 26)<br />
Promo<strong>Cell</strong> Fibroblast Growth Medium 3<br />
has been developed for the in vitro cultivation<br />
of adult Human Cardiac Fibroblasts<br />
(HCF). The medium is optimized<br />
for primary human cells but can also be<br />
used for porcine cardiac fibroblasts.<br />
Related products:<br />
Fetal calf serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
0.1 ml / ml<br />
1 ng / ml<br />
5 μg / ml<br />
4 Media and Sera<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Fibroblast Growth Medium 3<br />
(Ready-to-use)<br />
500 ml new C-23025<br />
Fibroblast Growth Medium 3 Kit 500 ml new C-23130<br />
Fibroblast Basal Medium 3 500 ml new C-23230<br />
Fibroblast Basal Medium 3,<br />
phenol red-free<br />
Fibroblast Growth Medium 3<br />
SupplementMix<br />
Fibroblast Growth Medium 3<br />
SupplementPack<br />
500 ml new C-23235<br />
for 500 ml new C-39345<br />
for 500 ml new C-39350
78<br />
4 Media and Sera<br />
Low Serum<br />
4.1.7 Follicle Dermal Papilla <strong>Cell</strong> Medium<br />
Follicle Dermal Papilla <strong>Cell</strong> Growth<br />
Medium<br />
Recommended for<br />
Human Follicle Dermal Papilla <strong>Cell</strong>s<br />
(see page 28)<br />
The Promo<strong>Cell</strong> Follicle Dermal Papilla<br />
<strong>Cell</strong> Growth Medium is a low-serum<br />
(4% V/V) medium. The combination of<br />
low serum and growth factors leads to<br />
excellent proliferation of human follicle<br />
dermal papilla cells in vitro.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
Bovine Pituitary Extract<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
0.04 ml / ml<br />
0.004 ml / ml<br />
1 ng / ml<br />
5 μg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
4 Media and Sera<br />
Follicle Dermal Papilla <strong>Cell</strong> Growth<br />
500 ml C-26501<br />
Medium (Ready-to-use)<br />
Follicle Dermal Papilla <strong>Cell</strong> Growth<br />
500 ml C-26502<br />
Medium Kit<br />
Follicle Dermal Papilla <strong>Cell</strong> Basal Medium 500 ml C-26500<br />
Follicle Dermal Papilla <strong>Cell</strong> Basal Medium,<br />
500 ml C-26505<br />
phenol red-free<br />
Follicle Dermal Papilla <strong>Cell</strong> Growth<br />
for 500 ml C-39625<br />
Medium SupplementMix<br />
Follicle Dermal Papilla <strong>Cell</strong> Growth<br />
Medium SupplementPack<br />
for 500 ml C-39620
4 Media and Sera 79<br />
Serum-Free<br />
4.1.8 Hepatocyte Media<br />
Hepatocyte Growth Medium<br />
Hepatocyte Maintenance Medium<br />
Recommended for<br />
Human Hepatocytes (see page 29)<br />
The Promo<strong>Cell</strong> Hepatocyte Growth Medium<br />
is a serum-free medium developed<br />
for the cultivation of human hepato-<br />
cytes. It is supplemented with well chosen<br />
growth factors and hormones and<br />
suited for long-term cultivation.<br />
In situations where the media compounds<br />
of the Hepatocyte Growth Medium interfere<br />
with certain experimental setups, we<br />
recommend using the Promo<strong>Cell</strong> Hepatocyte<br />
Maintenance Medium. This medium<br />
is developed for short-term cultivation<br />
and contains fewer supplements.<br />
Note: Under standard in vitro cell culture<br />
conditions, mature human hepatocytes<br />
usually do not survive for longer than<br />
10-14 days and do not proliferate.<br />
The media are optimized for primary human<br />
hepatocytes but can also be used<br />
for porcine hepatocytes.<br />
Final supplement concentrations<br />
(after addition to the medium) Hepatocyte Growth Medium Hepatocyte Maintenance Medium<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
10 ng / ml —<br />
Insulin (recombinant human) 5 μg / ml 6 ng / ml<br />
Hydrocortisone 0.5 μg / ml —<br />
Transferrin, holo (human) 10 μg / ml —<br />
Ascorbic Acid 250 µg / ml —<br />
Bovine Serum Albumin-Fatty Acid Free<br />
3.75 mg / ml —<br />
(BSA-FAF)<br />
Dexamethasone — 400 ng / ml<br />
https://www.promocell.com/shop<br />
4 Media and Sera<br />
Product Size Catalog Number<br />
Hepatocyte Growth Medium<br />
(Ready-to-use)<br />
500 ml C-25010<br />
Hepatocyte Growth Medium Kit 500 ml C-25110<br />
Hepatocyte Basal Medium 500 ml C-25210<br />
Hepatocyte Basal Medium,<br />
phenol red-free<br />
Hepatocyte Growth Medium<br />
SupplementMix<br />
Hepatocyte Growth Medium<br />
SupplementPack<br />
Hepatocyte Maintenance Medium<br />
(Ready-to-use)<br />
500 ml C-25215<br />
for 500 ml C-39642<br />
for 500 ml C-39640<br />
500 ml C-25020<br />
Hepatocyte Maintenance Medium Kit 500 ml C-25120<br />
Hepatocyte Maintenance Medium<br />
SupplementMix<br />
Hepatocyte Maintenance Medium<br />
SupplementPack<br />
for 500 ml C-39652<br />
for 500 ml C-39650
80<br />
4 Media and Sera<br />
Serum-Free<br />
4.1.9 Keratinocyte Medium<br />
Keratinocyte Growth Medium 2<br />
Recommended for<br />
Normal Human Epidermal<br />
Keratinocytes, juvenile foreskin<br />
(see page 30)<br />
Normal Human Epidermal<br />
Keratinocytes, adult donor<br />
(see page 30)<br />
Promo<strong>Cell</strong> Keratinocyte Growth Medium<br />
2 is a serum-free medium developed<br />
for the cultivation of epidermal keratinocytes<br />
without feeder cells.<br />
The medium is optimized for primary human<br />
cells but can also be used for horse<br />
and porcine keratinocytes, as well as<br />
mouse and rat keratinocytes after adapting<br />
the CaCl 2<br />
concentration. In addition,<br />
it is suited for rat corneal keratinocytes.<br />
Related products<br />
CaCl 2<br />
-Solution (see page 104)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
4 Media and Sera<br />
Bovine Pituitary Extract<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
Hydrocortisone<br />
Epinephrine<br />
Transferrin, holo (human)<br />
CaCl 2<br />
0.004 ml / ml<br />
0.125 ng / ml<br />
5 μg / ml<br />
0.33 μg / ml<br />
0.39 μg / ml<br />
10 μg / ml<br />
0.06 mM<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Keratinocyte Growth Medium 2<br />
(Ready-to-use)<br />
500 ml C-20011<br />
Keratinocyte Growth Medium 2 Kit 500 ml C-20111<br />
Keratinocyte Basal Medium 2 500 ml C-20211<br />
Keratinocyte Basal Medium 2,<br />
phenol red-free<br />
Keratinocyte Growth Medium 2<br />
SupplementMix<br />
Keratinocyte Growth Medium 2<br />
SupplementPack<br />
500 ml C-20216<br />
for 500 ml C-39016<br />
for 500 ml C-39011
4 Media and Sera 81<br />
Serum-Free<br />
4.1.10 Melanocyte Media<br />
Melanocyte Growth Medium<br />
Recommended for<br />
Normal Human Epidermal Melanocytes,<br />
juvenile foreskin (see page 31)<br />
The Promo<strong>Cell</strong> Melanocyte Growth<br />
Medium is a serum-free, PMA (Phorbol<br />
Myristate Acetate) containing medium<br />
optimized for the cultivation of human<br />
melanocytes. Since PMA is a tumor promoting<br />
mitogen, it may interfere with<br />
some experimental approaches. In these<br />
cases, we recommend using Melanocyte<br />
Growth Medium M2.<br />
The medium is optimized for primary human<br />
cells but can also be used for horse,<br />
porcine, murine, and rat melanocytes.<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Bovine Pituitary Extract<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
Hydrocortisone<br />
Phorbol Myristate Acetate<br />
0.004 ml / ml<br />
1 ng / ml<br />
5 μg / ml<br />
0.5 μg / ml<br />
10 ng / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Melanocyte Growth Medium (Ready-to-use) 500 ml C-24010<br />
Melanocyte Growth Medium Kit 500 ml C-24110<br />
Melanocyte Basal Medium 500 ml C-24210<br />
Melanocyte Basal Medium, phenol red-free 500 ml C-24215<br />
Melanocyte Growth Medium SupplementMix for 500 ml C-39415<br />
Melanocyte Growth Medium SupplementPack for 500 ml C-39410<br />
4 Media and Sera<br />
Serum-Free<br />
Melanocyte Growth Medium M2<br />
Recommended for<br />
Normal Human Epidermal<br />
Melanocytes, juvenile foreskin,<br />
cultured in M2 Medium (see page 31)<br />
Normal Human Epidermal<br />
Melanocytes, adult donor, cultured<br />
in M2 Medium (see page 31)<br />
The unique Promo<strong>Cell</strong> Melanocyte<br />
Growth Medium 2 is a serum-free and<br />
PMA (Phorbol Myristate Acetate) free<br />
medium optimized for the cultivation of<br />
human melanocytes.<br />
It is optimized for primary human cells<br />
but can also be used for equine, porcine,<br />
murine, and rat melanocytes. It is also<br />
suitable for human pigment epithelial<br />
cells, neuronal cells and melanoma cells.<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Melanocyte Growth Medium M2<br />
(Ready-to-use)<br />
Melanocyte Growth Medium M2<br />
(Ready-to-use), phenol red-free<br />
500 ml C-24300<br />
500 ml C-24305<br />
Melanocyte Basal Medium M2 500 ml C-24400<br />
Melanocyte Basal Medium M2,<br />
phenol red-free<br />
Melanocyte Growth Medium M2<br />
SupplementMix<br />
500 ml C-24405<br />
for 500 ml C-39420
82<br />
4 Media and Sera<br />
4.1.11 Osteoblast Media<br />
Osteoblast Growth Medium<br />
Recommended for<br />
Human Osteoblasts (see page 32)<br />
Promo<strong>Cell</strong> Osteoblast Growth Medium<br />
was developed to establish and maintain<br />
human osteoblast cultures. The<br />
fetal calf serum in the medium is strictly<br />
pretested for optimal performance.<br />
The media are optimized for primary human<br />
cells but can also be used for bovine,<br />
murine, and rat osteoblasts as well<br />
as osteoblast cell lines.<br />
Final supplement concentration<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
0.1 ml / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Osteoblast Growth Medium<br />
(Ready-to-use)<br />
500 ml C-27001<br />
Osteoblast Basal Medium 500 ml C-27010<br />
Osteoblast Basal Medium,<br />
phenol red-free<br />
Osteoblast Growth Medium<br />
SupplementMix<br />
500 ml C-27015<br />
for 500 ml C-39615<br />
4 Media and Sera<br />
Osteoblast Mineralization Medium<br />
Promo<strong>Cell</strong> Osteoblast Mineralization<br />
served after 21 days, following an easy<br />
Medium was developed for the effi-<br />
one-step protocol (see page 32, Fig. 3).<br />
Recommended for<br />
cient induction of strong mineralization<br />
in human osteoblast cultures. Extensive<br />
Human Osteoblasts (see page 32)<br />
extracellular calcium deposits can be ob-<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Osteoblast Mineralization Medium 100 ml C-27020
4 Media and Sera 83<br />
Low Serum<br />
4.1.12 Preadipocyte/Adipocyte Media<br />
Preadipocyte Growth Medium<br />
Preadipocyte Differentiation<br />
Medium<br />
Adipocyte Nutrition Medium<br />
Recommended for<br />
The Promo<strong>Cell</strong> Preadipocyte Growth<br />
Medium is a low-serum (5% V/V) medium<br />
optimized for the expansion of human<br />
preadipocytes.<br />
The Preadipocyte Differentiation Medium<br />
is a serum-free medium, which<br />
induces the differentiation of preadipocytes<br />
into mature adipocytes.<br />
The media are optimized for primary human<br />
cells but can also be used for murine<br />
preadipocytes/adipocytes.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Human White Preadipocytes<br />
(see page 33)<br />
The mature adipocytes can be cultivated<br />
using the Adipocyte Nutrition Medium.<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Preadipocyte<br />
Growth Medium<br />
Preadipocyte<br />
Differentiation Medium<br />
Adipocyte<br />
Nutrition Medium<br />
Fetal Calf Serum 0.05 ml / ml — 0.03 ml / ml<br />
Endothelial <strong>Cell</strong> Growth Supplement 0.004 ml / ml — —<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
10 ng / ml — —<br />
Hydrocortisone 1 μg / ml — —<br />
d-Biotin — 8 μg / ml 8 μg / ml<br />
Insulin (recombinant human) — 0.5 μg / ml 0.5 μg / ml<br />
Dexamethasone — 400 ng / ml 400 ng / ml<br />
IBMX — 44 μg / ml —<br />
L-Thyroxine — 9 ng / ml —<br />
Ciglitazone — 3 μg / ml —<br />
Heparin 90 μg / ml — —<br />
4 Media and Sera<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Preadipocyte Growth Medium (Ready-to-use) 500 ml C-27410<br />
Preadipocyte Growth Medium Kit 500 ml C-27417<br />
Preadipocyte Basal Medium 500 ml C-27411<br />
Preadipocyte Basal Medium,<br />
phenol red-free<br />
Preadipocyte Growth Medium<br />
SupplementMix<br />
Preadipocyte Growth Medium<br />
SupplementPack<br />
Preadipocyte Differentiation Medium<br />
(Ready-to-use)<br />
500 ml C-27415<br />
for 500 ml C-39425<br />
for 500 ml C-39427<br />
500 ml C-27436<br />
Preadipocyte Differentiation Medium Kit 500 ml C-27437<br />
Preadipocyte Differentiation Medium<br />
SupplementMix<br />
Preadipocyte Differentiation Medium<br />
SupplementPack<br />
for 500 ml C-39436<br />
for 500 ml C-39437<br />
Adipocyte Nutrition Medium (Ready-to-use) 500 ml C-27438<br />
Adipocyte Nutrition Medium Kit 500 ml C-27439<br />
Adipocyte Basal Medium 500 ml C-27431<br />
Adipocyte Basal Medium, phenol red-free 500 ml C-27435<br />
Adipocyte Nutrition Medium SupplementMix for 500 ml C-39438<br />
Adipocyte Nutrition Medium SupplementPack for 500 ml C-39439
84<br />
4 Media and Sera<br />
Low Serum<br />
4.1.13 Skeletal Muscle <strong>Cell</strong> Media<br />
Skeletal Muscle <strong>Cell</strong> Growth<br />
Medium<br />
Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium<br />
Recommended for<br />
Human Skeletal Muscle <strong>Cell</strong>s<br />
(see page 34)<br />
The Promo<strong>Cell</strong> Skeletal Muscle <strong>Cell</strong><br />
Growth Medium is a low-serum (5%<br />
V/V) medium optimized for the expansion<br />
of human skeletal muscle cells.<br />
The unique Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium is a serum-free<br />
medium that induces the fusion of<br />
myoblasts to myotubes with typical<br />
multinucleated syncytia.<br />
The media are optimized for primary human<br />
cells but can also be used for rat,<br />
murine, and rabbit skeletal muscle cells.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Skeletal Muscle <strong>Cell</strong><br />
Growth Medium<br />
Skeletal Muscle <strong>Cell</strong><br />
Differentiation Medium<br />
Fetal Calf Serum 0.05 ml / ml —<br />
Fetuin (bovine) 50 μg / ml —<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
10 ng / ml —<br />
4 Media and Sera<br />
Basic Fibroblast Growth Factor<br />
1 ng / ml —<br />
(recombinant human)<br />
Insulin (recombinant human) 10 μg / ml 10 μg / ml<br />
Dexamethasone 0.4 μg / ml —<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Skeletal Muscle <strong>Cell</strong> Growth Medium<br />
(Ready-to-use)<br />
500 ml C-23060<br />
Skeletal Muscle <strong>Cell</strong> Growth Medium Kit 500 ml C-23160<br />
Skeletal Muscle <strong>Cell</strong> Basal Medium 500 ml C-23260<br />
Skeletal Muscle <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
Skeletal Muscle <strong>Cell</strong> Growth Medium<br />
SupplementMix<br />
Skeletal Muscle <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium (Ready-to-use)<br />
Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium SupplementMix<br />
500 ml C-23265<br />
for 500 ml C-39365<br />
for 500 ml C-39360<br />
500 ml C-23061<br />
for 500 ml C-39366
4 Media and Sera 85<br />
Low Serum<br />
4.1.14 Smooth Muscle <strong>Cell</strong> Medium<br />
Smooth Muscle <strong>Cell</strong> Growth<br />
Medium 2<br />
Recommended for<br />
Human Umbilical Artery Smooth<br />
Muscle <strong>Cell</strong>s (see page 37)<br />
Human Coronary Artery Smooth<br />
Muscle <strong>Cell</strong>s (see page 36)<br />
Human Pulmonary Artery Smooth<br />
Muscle <strong>Cell</strong>s (see page 36)<br />
Human Aortic Smooth Muscle <strong>Cell</strong>s<br />
(see page 35)<br />
Human Bronchial Smooth Muscle<br />
<strong>Cell</strong>s (see page 38)<br />
Human Tracheal Smooth Muscle <strong>Cell</strong>s<br />
(see page 37)<br />
Human Bladder Smooth Muscle <strong>Cell</strong>s<br />
(see page 38)<br />
Human Prostate Smooth Muscle <strong>Cell</strong>s<br />
(see page 39)<br />
Human Uterine Smooth Muscle <strong>Cell</strong>s<br />
(see page 39)<br />
The Promo<strong>Cell</strong> Smooth Muscle <strong>Cell</strong><br />
Growth Medium 2 is a low-serum (5%<br />
V/V) medium developed to establish and<br />
maintain smooth muscle cell cultures.<br />
The medium is optimized for primary<br />
human cells but can also be used for<br />
bovine, porcine, murine, dog, and rat<br />
smooth muscle cells.<br />
Related products<br />
Fetal Calf Serum (see page 92)<br />
Final supplement concentrations<br />
(after addition to the medium)<br />
Fetal Calf Serum<br />
Epidermal Growth Factor<br />
(recombinant human)<br />
Basic Fibroblast Growth Factor<br />
(recombinant human)<br />
Insulin (recombinant human)<br />
0.05 ml / ml<br />
0.5 ng / ml<br />
2 ng / ml<br />
5 μg / ml<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />
500 ml C-22062<br />
(Ready-to-use)<br />
Smooth Muscle <strong>Cell</strong> Growth Medium 2 Kit 500 ml C-22162<br />
Smooth Muscle <strong>Cell</strong> Basal Medium 2 500 ml C-22262<br />
Smooth Muscle <strong>Cell</strong> Basal Medium 2,<br />
500 ml C-22267<br />
phenol red-free<br />
Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />
SupplementMix<br />
for 500 ml C-39267<br />
4 Media and Sera<br />
Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />
SupplementPack<br />
for 500 ml C-39262<br />
4.1.15 Freezing Medium<br />
Serum-Free<br />
Recommended for<br />
Human Primary <strong>Cell</strong>s<br />
Animal Primary <strong>Cell</strong>s<br />
<strong>Cell</strong> Lines<br />
The Promo<strong>Cell</strong> Cryo-SFM is an animal<br />
component-free, chemically defined medium<br />
with DMSO, methylcellulose, and<br />
other cryoprotective components developed<br />
for the cryopreservation of human<br />
and animal primary cells. It ensures high<br />
cell viability, excellent growth rates, and<br />
good attachment of the cells after thawing.<br />
The medium is the first choice for all<br />
types of primary cells and cell lines.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Cryo-SFM<br />
30 ml<br />
125 ml<br />
C-29910<br />
C-29912
86<br />
4 Media and Sera<br />
4.2. Media for Stem and Blood <strong>Cell</strong>s<br />
The Promo<strong>Cell</strong> media for stem and blood<br />
cells are formulated for either the optimal<br />
growth of specific cell types or to<br />
support the differentiation into the respective<br />
mature cells.<br />
Each lot is tested for its ability to support<br />
growth or differentiation, respectively. In<br />
addition, biochemical and sterility tests<br />
are performed*.<br />
The Media (Ready-to-use) consist of a<br />
100 ml, 250 ml, or 500 ml bottle of Basal<br />
Medium and a vial of SupplementMix.<br />
Adding the SupplementMix to the Basal<br />
Medium results in the complete medium.<br />
Some Media are provided with additional<br />
Cytokine Mixes to achieve optimal<br />
performance.<br />
The shelf life of the Basal Medium is<br />
9 months (at 4 to 8°C), and that of the<br />
SupplementMix is 12 months (at -20°C)<br />
from the date of manufacture. After<br />
adding the SupplementMix, the shelf life<br />
of the complete medium is 6 weeks (at<br />
4 to 8°C).<br />
Note: The media for stem and blood cells<br />
do not contain antibiotics.<br />
If you require special media modifications we offer a Custom Media Service starting at 10 bottles per order.<br />
Please ask for details.<br />
4.2.1 Mesenchymal Stem <strong>Cell</strong> Media<br />
4 Media and Sera<br />
Mesenchymal Stem <strong>Cell</strong><br />
Growth Medium<br />
Mesenchymal Stem <strong>Cell</strong><br />
Growth Medium DXF<br />
Mesenchymal Stem <strong>Cell</strong><br />
Differentiation Media<br />
The Promo<strong>Cell</strong> Mesenchymal Stem <strong>Cell</strong><br />
Growth Medium supports the expansion<br />
of multipotent human Mesenchymal<br />
Stem <strong>Cell</strong>s (MSC) without inducing early<br />
senescence and differentiation as observed<br />
in standard culture media.<br />
The Mesenchymal Stem <strong>Cell</strong> Growth<br />
Medium DXF allows for the robust<br />
isolation from human bone marrow is<br />
supported as well.<br />
In addition, Promo<strong>Cell</strong> offers five<br />
media to efficiently induce differentiation<br />
of MSC into adipogenic, chondrogenic,<br />
osteogenic or neurogenic lineages, respectively<br />
(see also Fig. 3, page 48).<br />
proliferation of multipotent MSC in a<br />
Recommended for<br />
chemically defined and xeno-free culture<br />
environment. It is devoid of all animal-<br />
Human Mesenchymal Stem <strong>Cell</strong>s from<br />
derived components and substances<br />
Bone Marrow, Umbilical Cord Matrix,<br />
of human origin with Human Serum<br />
and Adipose Tissue (see page 47)<br />
Albumin as the only exception. Primary<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Mesenchymal Stem <strong>Cell</strong> Growth Medium<br />
(Ready-to-use)<br />
Mesenchymal Stem <strong>Cell</strong> Growth Medium<br />
DXF (Ready-to-use)<br />
Mesenchymal Stem <strong>Cell</strong> Adipogenic<br />
Differentiation Medium (Ready-to-use)<br />
Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium (Ready-to-use)<br />
Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium w/o Inducers<br />
(Ready-to-use)<br />
Mesenchymal Stem <strong>Cell</strong> Osteogenic<br />
Differentiation Medium (Ready-to-use)<br />
Mesenchymal Stem <strong>Cell</strong> Neurogenic<br />
Differentiation Medium (Ready-to-use)<br />
500 ml C-28010<br />
500 ml<br />
new<br />
C-28019<br />
100 ml C-28011<br />
100 ml C-28012<br />
100 ml C-28014<br />
100 ml C-28013<br />
100 ml C-28015<br />
*Certificates of analysis are available on request.
4 Media and Sera 87<br />
4.2.2 Pericyte Medium<br />
Pericyte Growth Medium<br />
Recommended for<br />
Human Pericytes (see page 49)<br />
Promo<strong>Cell</strong> Pericyte Growth Medium has<br />
been developed and optimized for the in<br />
vitro cultivation of human pericytes from<br />
all tissues.<br />
Whereas in standard culture media pericytes<br />
tend to grow slowly and induce<br />
early senescence, Promo<strong>Cell</strong> Pericyte<br />
Growth Medium optimally supports cell<br />
proliferation and maintenance of multipotency<br />
in these cells.<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Pericyte Growth Medium (Ready-to-use) 500 ml C-28040<br />
4.2.3 Hematopoietic Progenitor <strong>Cell</strong> Media<br />
Hematopoietic Progenitor <strong>Cell</strong><br />
sion of primitive HPCs in a fully defined,<br />
defined formula contains recombinant hu-<br />
Expansion Medium DXF<br />
Hematopoietic Progenitor Medium<br />
xeno-free culture environment. Due to<br />
the utilization of exclusively synthetic,<br />
recombinant or plant-sourced materials,<br />
man TPO, SCF, flt-3 ligand and IL-3. The<br />
optimized formulation allows vigorous<br />
expansion of hematopoietic progenitor<br />
Recommended for<br />
Human Mononuclear <strong>Cell</strong>s from<br />
Cord Blood (see page 54)<br />
this medium is chemically defined and<br />
free of all animal-derived components<br />
and substances of human origin with<br />
Human Serum Albumin as the only<br />
exception.<br />
cells, while maintaining their primitive<br />
phenotype. Differentiation is largely<br />
inhibited.<br />
The Promo<strong>Cell</strong> Hematopoietic Pro-<br />
4 Media and Sera<br />
Human CD34 + Progenitor <strong>Cell</strong>s<br />
genitor Medium has been developed<br />
(see page 50)<br />
Note: The medium must be supple-<br />
for the expansion of hematopoietic<br />
Human CD133 + Progenitor <strong>Cell</strong>s<br />
mented with additional cytokines, e.g.<br />
progenitors by means of differentiation<br />
(see page 51)<br />
Cytokine Mix E (see below). Individual<br />
into more committed lineages of blood<br />
Human Hematopoietic Progenitor<br />
cytokines can be found in the PromoKine<br />
cells. Hence, this progenitor medium<br />
<strong>Cell</strong>s<br />
catalog.<br />
is suitable for CFU-based assays, e.g.<br />
long-term culture initiating cell (LTC-IC)<br />
The Promo<strong>Cell</strong> Hematopoietic Progenitor<br />
Cytokine Mix E is a ready-to-use mixture<br />
assays.<br />
<strong>Cell</strong> (HPC) Expansion Medium DXF has<br />
of growth factors for the HPC Expansion<br />
been designed for the maximum expan-<br />
Medium DXF. The animal component-free,<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Hematopoietic Progenitor <strong>Cell</strong><br />
Expansion Medium DXF<br />
500 ml C-28021<br />
Cytokine Mix E<br />
for HPC Expansion Medium DXF<br />
1 ml (sufficient for 100 ml Medium)<br />
5 ml (sufficient for 500 ml Medium)<br />
C-39890<br />
C-39891<br />
Hematopoietic Progenitor Medium<br />
(Ready-to-use)<br />
100 ml C-28020
88<br />
4 Media and Sera<br />
4.2.4 Endothelial Progenitor Medium<br />
Endothelial Progenitor Medium<br />
Recommended for<br />
Human Mononuclear <strong>Cell</strong>s<br />
(see page 54)<br />
Human CD34 + Progenitor <strong>Cell</strong>s<br />
(see page 50)<br />
Human Endothelial Progenitor <strong>Cell</strong>s<br />
The Promo<strong>Cell</strong> Endothelial Progenitor<br />
Medium has been developed for the<br />
expansion of endothelial progenitors by<br />
means of differentiation into more committed<br />
lineages of endothelial cells.<br />
Hence, this progenitor medium is suitable<br />
for the differentiation of primitive<br />
progenitors into mature endothelial cells<br />
as well as for CFU-based assays, e.g. the<br />
“Hill-Assay” for endothelial progenitor<br />
cells (Hill et al. 2003, N Engl J Med<br />
348: 593-600), which is carried out on<br />
fibronectin-coated plates.<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Endothelial Progenitor Medium<br />
(Ready-to-use)<br />
100 ml C-28025<br />
4.2.5 Dendritic <strong>Cell</strong> Media<br />
4 Media and Sera<br />
DC Generation Medium<br />
DC Generation Medium DXF<br />
Monocyte Attachment Medium<br />
Promo<strong>Cell</strong> DC Generation Medium allows<br />
for the generation of DC from<br />
cryopreserved as well as freshly isolated<br />
cells. In the latter case, the Monocyte Attachment<br />
Medium is also needed.<br />
The Monocyte Attachment Medium<br />
allows for the efficient adherence selection<br />
of Monocytes from freshly isolated<br />
Mononuclear <strong>Cell</strong>s (MNC) while<br />
maintaining optimal cell health. Thus,<br />
the time-consuming and costly immuno-<br />
Recommended for<br />
The DC Generation Medium DXF is a<br />
magnetic purification of monocytes prior<br />
chemically defined and xeno-free for-<br />
to dendritic cell generation is not neces-<br />
Human Monocytes, freshly isolated<br />
mulation for use with freshly isolated<br />
sary and can be skipped.<br />
and cryopreserved (see page 52)<br />
cells. It is free of all animal derived com-<br />
Human Mononuclear <strong>Cell</strong>s, freshly<br />
ponents and substances of human origin<br />
Note: See our Instruction Manual and<br />
isolated<br />
with Human Serum Albumin as the only<br />
Application Note for details. Please visit<br />
exception.<br />
www.promocell.com/application-notes<br />
The Promo<strong>Cell</strong> Dendritic <strong>Cell</strong> (DC) Generation<br />
Media have been developed for the<br />
The DC Base Media do not include cy-<br />
easy and efficient generation of immature<br />
tokines and must therefore be supple-<br />
as well as fully mature myeloid Dendritic<br />
mented according to the user’s individual<br />
<strong>Cell</strong>s from peripheral blood monocytes.<br />
needs.<br />
For more information please see<br />
www.promocell.com/knowledge-base
4 Media and Sera 89<br />
List of supplements and their concentrations: confidential<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
DC Generation Medium (Ready-to-use) 250 ml<br />
new<br />
C-28050<br />
DC Generation Medium DXF<br />
(Ready-to-use)<br />
250 ml new C-28052<br />
DC Base Medium 250 ml new C-28053<br />
DC Base Medium DXF 250 ml new C-28054<br />
Monocyte Attachment Medium<br />
(Ready-to-use)<br />
250 ml new C-28051<br />
4.2.6 Mononuclear <strong>Cell</strong> Medium<br />
Mononuclear <strong>Cell</strong> Medium<br />
Human Monocytes (see page 52)<br />
Recommended for<br />
The Promo<strong>Cell</strong> Mononuclear <strong>Cell</strong> Medium<br />
is a very rich short-term maintenance<br />
medium which provides utmost<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood (see page 54) cell viability.<br />
Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood (see page 54) It is suitable to maintain MNCs for up to<br />
List of supplements and their concentrations: confidential<br />
48 hours before proceeding with your<br />
experiments.<br />
Related products<br />
Lymphocyte Separation Medium<br />
1077 (see below)<br />
4 Media and Sera<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Mononuclear <strong>Cell</strong> Medium (Ready-to-use) 500 ml C-28030<br />
4.2.7 Blood <strong>Cell</strong> Separation Medium<br />
Lymphocyte Separation Medium<br />
1077<br />
Recommended for<br />
Peripheral blood<br />
Umbilical cord blood<br />
Buffy coats<br />
Apheresis products<br />
Bone marrow<br />
The Promo<strong>Cell</strong> Lymphocyte Separation<br />
Medium 1077 is a sterile, ready-to-use<br />
solution optimized for the separation of<br />
lymphocytes from whole blood or buffy<br />
coats without any loss of viability. It is<br />
based on Ficoll 400 and sodium diatrizoate<br />
and provides optimal physiological<br />
parameters. It has a density of 1,077 g/ml<br />
at +20°C.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Lymphocyte Separation Medium 1077 500 ml C-44010
90<br />
4 Media and Sera<br />
4.3 Classical Media<br />
• DMEM Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
DMEM, w/o L-Glutamine 500 ml C-71110<br />
DMEM, with L-Glutamine 500 ml C-71111<br />
DMEM, with 25 mM HEPES,<br />
500 ml C-71115<br />
w/o L-Glutamine<br />
DMEM, with 25 mM HEPES,<br />
500 ml C-71116<br />
with L-Glutamine<br />
DMEM, with stable Glutamine 500 ml C-71120<br />
DMEM, High Glucose (4.5 g/l),<br />
500 ml C-71210<br />
w/o L-Glutamine<br />
DMEM, High Glucose (4.5 g/l),<br />
500 ml C-71212<br />
with L-Glutamine<br />
DMEM, High Glucose (4.5 g/l),<br />
500 ml C-71220<br />
with stable Glutamine<br />
DMEM, High Glucose (4.5 g/l),<br />
500 ml C-71221<br />
with stable Glutamine, with Na-Pyruvate<br />
DMEM, High Glucose (4.5 g/l),<br />
500 ml C-71222<br />
with L-Glutamine, with Na-Pyruvate<br />
DMEM, High Glucose (4.5 g/l),<br />
w/o L-Glutamine, with Na-Pyruvate<br />
500 ml C-71223<br />
4 Media and Sera<br />
• DMEM/Ham´s F-12 Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
DMEM/Ham´s F-12, w/o L-Glutamine 500 ml C-71310<br />
DMEM/Ham´s F-12, with L-Glutamine 500 ml C-71311<br />
DMEM/Ham´s F-12, with stable Glutamine 500 ml C-71320<br />
• Ham´s Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Ham´s F-10, w/o L-Glutamine 500 ml C-72010<br />
Ham´s F-10, with L-Glutamine 500 ml C-72020<br />
Ham´s F-12, w/o L-Glutamine 500 ml C-72110<br />
Ham´s F-12, with L-Glutamine 500 ml C-72120<br />
• Leibovitz L-15 Medium<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Leibovitz L-15, w/o L-Glutamine 500 ml C-73110
4 Media and Sera 91<br />
• McCoy´s 5A Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
McCoy’s 5A, w/o L-Glutamine 500 ml C-73210<br />
McCoy’s 5A, with L-Glutamine 500 ml C-73211<br />
McCoy´s 5A, with stable Glutamine 500 ml C-73220<br />
McCoy´s 5A, with stable Glutamine,<br />
500 ml C-73227<br />
phenol red-free<br />
• MEM Alpha and MEM D-Valine Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
MEM Alpha, w/o Nucleosides,<br />
500 ml C-75012<br />
w/o L-Glutamine<br />
MEM Alpha, w/o Nucleosides,<br />
500 ml C-75013<br />
with L-Glutamine<br />
MEM Alpha, with Nucleosides,<br />
500 ml C-75014<br />
with L-Glutamine<br />
MEM D-Valine, w/o L-Glutamine 500 ml C-75100<br />
• MEM Earle Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
4 Media and Sera<br />
MEM Earle, w/o L-Glutamine 500 ml C-75210<br />
MEM Earle, with L-Glutamine 500 ml C-75211<br />
MEM Earle, with stable Glutamine 500 ml C-75220<br />
• RPMI 1640 Media<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
RPMI 1640, w/o L-Glutamine 500 ml C-76010<br />
RPMI 1640, with L-Glutamine 500 ml C-76011<br />
RPMI 1640, with 25 mM HEPES,<br />
500 ml C-76015<br />
w/o L-Glutamine<br />
RPMI 1640, with 25 mM HEPES,<br />
500 ml C-76016<br />
with L-Glutamine<br />
RPMI 1640, w/o L-Glutamine,<br />
500 ml C-76017<br />
phenol red-free<br />
RPMI 1640, with stable Glutamine 500 ml C-76020
92<br />
4 Media and Sera<br />
4.4 Insect <strong>Cell</strong> <strong>Culture</strong> Media<br />
The medium requirements of insect cells<br />
are significantly different from vertebrate<br />
cell culture. For instance, the optimal pH<br />
range for most insect cells is more acidic<br />
(pH 6.2 to 6.5). Also, the amino acid<br />
concentration needs to be higher than in<br />
vertebrate cell media.<br />
Mitsuhashi/Maramorosh Insect<br />
Medium<br />
Recommended for<br />
Mitsuhashi and Maramorosh Insect<br />
Medium is well-suited for Mosquito cell<br />
culture and must be supplemented with<br />
FCS prior to use.<br />
Mosquito <strong>Cell</strong>s<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Mitsuhashi/Maramorosh Insect Medium 500 ml C-77110<br />
4 Media and Sera<br />
Schneider’s Drosophila Medium<br />
Recommended for<br />
Dipteran <strong>Cell</strong>s<br />
Schneider’s Drosophila Medium was<br />
originally developed for the culture of<br />
Drosophila cells but can also be used for<br />
the culture of other dipteran cell lines.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Schneider’s Drosophila Medium,<br />
w/o L-Glutamine<br />
500 ml C-77210<br />
4.5 Sera<br />
Fetal Calf Serum<br />
Recommended for<br />
Primary Human <strong>Cell</strong>s (all types)<br />
The Promo<strong>Cell</strong> Fetal Calf Sera are high<br />
quality, pretested fetal calf serum batches.<br />
They are extensively tested for their<br />
ability to support in vitro expansion of<br />
human primary cells.<br />
In addition, histochemical staining is performed<br />
to ensure that the cultured primary<br />
cells maintain their cell type specific<br />
marker expression.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Fetal Calf Serum (EU Quality)<br />
primary human cell culture tested<br />
100 ml<br />
500 ml<br />
C-37355<br />
C-37350
4 Media and Sera 93<br />
MSC-Qualified Fetal Calf Serum<br />
Recommended for<br />
Mesenchymal Stem <strong>Cell</strong>s (all types)<br />
The Promo<strong>Cell</strong> MSC-Qualified Fetal Calf<br />
Sera are high quality, pre-selected fetal<br />
calf serum batches. They are extensively<br />
tested for their ability to support in vitro<br />
expansion of human Mesenchymal Stem<br />
<strong>Cell</strong>s. In addition, histochemical staining<br />
is performed to ensure that the cultured<br />
stem cells maintain the ability to differentiate<br />
into diverse lineages.<br />
Fig. 1: Expansion and differentiation of<br />
Mesenchymal Stem <strong>Cell</strong>s (MSC) using<br />
media with the Promo<strong>Cell</strong> MSC-Qualified<br />
Fetal Calf Serum. Left: Undifferentiated<br />
hMSC-BM cultured for 4 passages<br />
(phase contrast). Right: The same cells<br />
after 14 days of adipogenic induction<br />
(Oil Red O staining of lipid droplets<br />
with Hematoxylin counterstain).<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
MSC-Qualified Fetal Calf Serum<br />
100 ml<br />
500 ml<br />
C-37386<br />
C-37385<br />
ESC-Qualified Fetal Calf Serum<br />
The Promo<strong>Cell</strong> ESC-Qualified Fetal Calf<br />
factor. The maintenance of pluripotency<br />
4 Media and Sera<br />
Sera are high quality, pre-selected fetal<br />
is assayed by immunostaining for basal<br />
Recommended for<br />
calf serum batches. They are exten-<br />
pluripotency markers. In addition, this<br />
sively tested for their ability to support<br />
serum is also recommended for the cul-<br />
Pluripotent Embryonic Stem <strong>Cell</strong>s<br />
undifferentiated, feeder-free culture of<br />
ture of hematopoietic and endothelial<br />
Hematopoietic Progenitor <strong>Cell</strong>s<br />
pluripotent murine embryonic stem cells<br />
progenitor cells.<br />
Endothelial Progenitor <strong>Cell</strong>s<br />
in the presence of leukemia inhibitory<br />
Fig. 2: Growth morphology of murine<br />
embryonic stem cells (mESC). Left:<br />
mESC in standard Fetal Calf Serum.<br />
Note the large, flat, differentiated<br />
cells. Right: mESC after 5 passages in<br />
Promo<strong>Cell</strong> ESC-Qualified Fetal Calf<br />
Serum. The cells are small and have a<br />
compact colony morphology typical for<br />
mESC.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
ESC-Qualified Fetal Calf Serum<br />
100 ml<br />
500 ml<br />
C-37388<br />
C-37387
<strong>Cell</strong> Pellets<br />
5<br />
Promo<strong>Cell</strong> <strong>Cell</strong> Pellets are an easily accessible source of RNA,<br />
DNA, or proteins from a wide range of human primary cell<br />
types without laborious cell cultivation work. This allows you<br />
to focus on your molecular biology experiments and maximize<br />
your valuable research time.
96<br />
Content Chapter 5<br />
5<br />
<strong>Cell</strong> Pellets<br />
5.1 <strong>Cell</strong> Pellets new ...................... 97
5 <strong>Cell</strong> Pellets 97<br />
5.1 <strong>Cell</strong> Pellets<br />
Applications<br />
Gene expression analysis<br />
<strong>Cell</strong> characterization studies<br />
Northern blots<br />
Southern blots<br />
Western blots/2D gel electrophoresis<br />
Features<br />
Available from all Promo<strong>Cell</strong><br />
cell types<br />
1 million cells per pellet<br />
Stored in RNAlater ®<br />
Indefinitely stable at -20°C<br />
Compatible with established nucleic<br />
acid purification protocols<br />
Promo<strong>Cell</strong> <strong>Cell</strong> Pellets are stored in<br />
RNAlater ® - an aqueous protection solution<br />
that rapidly permeates the cells and stabilizes<br />
cellular DNA, RNA, and proteins<br />
and protects them against degradation.<br />
This makes the Promo<strong>Cell</strong> <strong>Cell</strong> Pellets<br />
very useful for a wide range of research<br />
applications* like gene expression analysis,<br />
cell characterization studies, and protein<br />
characterization experiments.<br />
Note: RNAlater ® denatures proteins.<br />
Therefore, proteins will be suitable for<br />
e.g. Western blotting but not for applications<br />
that require native proteins.<br />
Each <strong>Cell</strong> Pellet is made from 1 million<br />
cells and is dissolved in 200 µl RNAlater ® .<br />
The <strong>Cell</strong> Pellets are produced from released<br />
cell lots and have the same characteristics,<br />
i.e. expression profile or cell<br />
type specific antigens. On request the<br />
cells from the same lot are available for<br />
cultivation. The <strong>Cell</strong> Pellets can be stored<br />
indefinitely at -20°C.<br />
https://www.promocell.com/shop<br />
Product Product Description Catalog Number<br />
HAoAF Pellet Pellet from Human Aortic Adventitial Fibroblasts C-14037<br />
HAoEC Pellet Pellet from Human Aortic Endothelial <strong>Cell</strong>s C-14023<br />
HAoSMC Pellet Pellet from Human Aortic Smooth Muscle <strong>Cell</strong>s C-14053<br />
HBdMEC Pellet Pellet from Human Bladder Microvascular Endothelial <strong>Cell</strong>s C-14026<br />
HBdSMC Pellet Pellet from Human Bladder Smooth Muscle <strong>Cell</strong>s C-14057<br />
HBEpC Pellet Pellet from Human Bronchial Epithelial <strong>Cell</strong>s C-14063<br />
HBMEC Pellet Pellet from Human Brain Microvascular Endothelial <strong>Cell</strong>s coming soon C-14032<br />
HBSMC Pellet Pellet from Human Bronchial Smooth Muscle <strong>Cell</strong>s C-14055<br />
HCAEC Pellet Pellet from Human Coronary Artery Endothelial <strong>Cell</strong>s C-14022<br />
HCASMC Pellet Pellet from Human Coronary Artery Smooth Muscle <strong>Cell</strong>s C-14052<br />
HCF Pellet Pellet from Human Cardiac Fibroblasts C-14036<br />
HCH Pellet Pellet from Human Chondrocytes C-14070<br />
HCM Pellet Pellet from Human Cardiac Myocytes C-14080<br />
HCMEC Pellet Pellet from Human Cardiac Microvascular Endothelial <strong>Cell</strong>s C-14029<br />
HDBEC adult Pellet Pellet from Human Dermal Blood Endothelial <strong>Cell</strong>s adult donor C-14019<br />
HDBEC Pellet<br />
Pellet from Human Dermal Blood Endothelial <strong>Cell</strong>s<br />
C-14018<br />
juvenile foreskin<br />
HDLEC adult Pellet<br />
Pellet from Human Dermal Lymphatic Endothelial <strong>Cell</strong>s<br />
C-14021<br />
adult donor<br />
HDLEC Pellet<br />
Pellet from Human Dermal Lymphatic Endothelial <strong>Cell</strong>s<br />
C-14020<br />
juvenile foreskin<br />
HDMEC adult Pellet<br />
Pellet from Human Dermal Microvascular Endothelial <strong>Cell</strong>s<br />
C-14016<br />
adult donor<br />
HDMEC Pellet<br />
Pellet from Human Dermal Microvascular Endothelial <strong>Cell</strong>s<br />
C-14015<br />
juvenile foreskin<br />
HDMEC pre-screened Pellet<br />
Pellet from Human Dermal Microvascular Endothelial <strong>Cell</strong>s<br />
C-14017<br />
pre-screened<br />
HFDPC Pellet Pellet from Human Follicle Dermal Papilla <strong>Cell</strong>s C-14005<br />
HHpC Pellet Pellet from Human Hepatocytes C-14085<br />
HMEpC Pellet Pellet from Human Mammary Epithelial <strong>Cell</strong>s C-14066<br />
hMNC-CB pooled Pellet<br />
Pellet from Human Mononuclear <strong>Cell</strong>s from Cord Blood<br />
C-14097<br />
pooled, ultra-pure<br />
hMNC-CB single donor Pellet<br />
Pellet from Human Mononuclear <strong>Cell</strong>s from Cord Blood<br />
C-14096<br />
single donor, ultra-pure<br />
hMNC-PB single donor Pellet<br />
Pellet from Human Mononuclear <strong>Cell</strong>s from Peripheral Blood<br />
C-14098<br />
single donor, ultra-pure<br />
hMNC-PB pooled Pellet<br />
Pellet from Human Mononuclear <strong>Cell</strong>s from Peripheral Blood<br />
pooled, ultra-pure<br />
new C-14099<br />
5 <strong>Cell</strong> Pellets<br />
* More information on RNAlater ® (FAQs, protocols) at www.invitrogen.com/site/us/en/home/brands/Product-Brand/rnalater.html
98<br />
5 <strong>Cell</strong> Pellets<br />
https://www.promocell.com/shop<br />
Product Product Description Catalog Number<br />
5 <strong>Cell</strong> Pellets<br />
hMoCD14 + -PB single donor Pellet<br />
hMoCD14 + -PB single donor<br />
pre-screened Pellet<br />
hMo-PB single donor Pellet<br />
hMo-PB single donor<br />
pre-screened Pellet<br />
Pellet from Human CD14 + Monocytes from Peripheral Blood,<br />
single donor<br />
Pellet from Human CD14 + Monocytes from Peripheral Blood,<br />
single donor pre-screened<br />
Pellet from Human Monocytes from Peripheral Blood, single<br />
donor<br />
Pellet from Human Monocytes from Peripheral Blood, single<br />
donor pre-screened<br />
C-14110<br />
C-14112<br />
C-14111<br />
C-14113<br />
hMSC-AT Pellet Pellet from Human Mesenchymal Stem <strong>Cell</strong>s from Adipose Tissue C-14092<br />
hMSC-BM Pellet Pellet from Human Mesenchymal Stem <strong>Cell</strong>s from Bone Marrow C-14090<br />
hMSC-UC Pellet<br />
Pellet from Human Mesenchymal Stem <strong>Cell</strong>s<br />
C-14091<br />
from Umbilical Cord Matrix<br />
HNEpC Pellet Pellet from Human Nasal Epithelial <strong>Cell</strong>s C-14062<br />
HOB Pellet Pellet from Human Osteoblasts C-14071<br />
HPAEC Pellet Pellet from Human Pulmonary Artery Endothelial <strong>Cell</strong>s C-14024<br />
HPASMC Pellet Pellet from Human Pulmonary Artery Smooth Muscle <strong>Cell</strong>s C-14051<br />
hPC-PL Pellet Pellet from Human Pericytes from Placenta C-14095<br />
HPF Pellet Pellet from Human Pulmonary Fibroblasts C-14035<br />
HPlEpC Pellet Pellet from Human Placental Epithelial <strong>Cell</strong>s C-14069<br />
HPMEC Pellet Pellet from Human Pulmonary Microvascular Endothelial <strong>Cell</strong>s C-14027<br />
HPrSMC Pellet Pellet from Human Prostate Smooth Muscle <strong>Cell</strong>s C-14058<br />
HRCEpC Pellet Pellet from Human Renal Cortical Epithelial <strong>Cell</strong>s C-14068<br />
HREpC Pellet Pellet from Human Renal Epithelial <strong>Cell</strong>s C-14067<br />
HSAEpC Pellet Pellet from Human Small Airway Epithelial <strong>Cell</strong>s C-14065<br />
HSaVEC Pellet Pellet from Human Saphenous Vein Endothelial <strong>Cell</strong>s C-14025<br />
HTEpC Pellet Pellet from Human Tracheal Epithelial <strong>Cell</strong>s C-14064<br />
HTSMC Pellet Pellet from Human Tracheal Smooth Muscle <strong>Cell</strong>s C-14056<br />
HUAEC Pellet Pellet from Human Umbilical Artery Endothelial <strong>Cell</strong>s C-14013<br />
HUASMC Pellet Pellet from Human Umbilical Artery Smooth Muscle <strong>Cell</strong>s C-14050<br />
HUF Pellet Pellet from Human Uterine Fibroblasts C-14038<br />
HUtMEC Pellet Pellet from Human Uterine Microvascular Endothelial <strong>Cell</strong>s C-14028<br />
HUtSMC Pellet Pellet from Human Uterine Smooth Muscle <strong>Cell</strong>s C-14059<br />
HUVEC Pellet Pellet from Human Umbilical Vein Endothelial <strong>Cell</strong>s C-14010<br />
HUVEC pooled Pellet Pellet from Human Umbilical Vein Endothelial <strong>Cell</strong>s pooled C-14011<br />
HUVEC pre-screened Pellet Pellet from Human Umbilical Vein Endothelial <strong>Cell</strong>s pre-screened C-14012<br />
HVMF Pellet Pellet from Human Villous Mesenchymal Fibroblasts C-14034<br />
HWP subcutaneous Pellet Pellet from Human White Preadipocytes subcutaneous C-14072<br />
HWP visceral Pellet Pellet from Human White Preadipocytes visceral C-14073<br />
NHDF adult Pellet Pellet from Normal Human Dermal Fibroblasts adult donor C-14031<br />
NHDF Pellet Pellet from Normal Human Dermal Fibroblasts juvenile foreskin C-14030<br />
NHEK adult Pellet Pellet from Normal Human Epidermal Keratinocytes adult donor C-14002<br />
NHEK adult pooled Pellet<br />
Pellet from Normal Human Epidermal Keratinocytes adult,<br />
new C-14004<br />
pooled<br />
NHEK.f Pellet<br />
Pellet from Normal Human Epidermal Keratinocytes<br />
C-14001<br />
juvenile foreskin<br />
NHEK.f pooled Pellet<br />
Pellet from Normal Human Epidermal Keratinocytes<br />
new C-14003<br />
juvenile foreskin, pooled<br />
NHEM M2 adult Pellet<br />
Pellet from Normal Human Epidermal Melanocytes<br />
C-14043<br />
adult donor, cultured in M2 Medium<br />
NHEM.f M2 Pellet<br />
Pellet from Normal Human Epidermal Melanocytes<br />
C-14042<br />
juvenile foreskin, cultured in M2 Medium<br />
NHEM.f Pellet<br />
Pellet from Normal Human Epidermal Melanocytes<br />
C-14040<br />
juvenile foreskin<br />
SkMC Pellet Pellet from Human Skeletal Muscle <strong>Cell</strong>s C-14060<br />
new<br />
new<br />
new<br />
new
Reagents and Supplements<br />
6<br />
Promo<strong>Cell</strong> offers a broad range of reagents and supplements<br />
for cell culture. This includes enzyme mixtures for tissue and<br />
cell dissociation, buffers for daily cell culture work, antibiotics<br />
and antimycotics in different concentrations, components<br />
to supplement various culture media, such as cytokines and<br />
growth factors, and reagents needed for cell assays. All of these<br />
are intensively tested and meet our stringent quality criteria.
100<br />
Content Chapter 6<br />
6<br />
Reagents and Supplements<br />
6.1 Detachment Reagents new ............ 101<br />
6.2 Antibiotics and Antimycotics .......... 103<br />
6.3 Buffers and Salt Solutions ............ 104<br />
6.4 Supplements new ................... 105<br />
6.5 Cytokines and Growth Factors. ........ 106
6 Reagents and Supplements 101<br />
6.1 Detachment Reagents<br />
DetachKit<br />
Recommended for<br />
The DetachKit is optimized for primary<br />
human cells but can also be used for primary<br />
animal cells and cell lines.<br />
All human primary cell types<br />
The Promo<strong>Cell</strong> DetachKit was developed<br />
for gentle and effective detachment of<br />
adherent primary human cells in culture.<br />
Each batch is tested on primary human cells.<br />
The kit contains three components:<br />
HepesBSS (30 mM Hepes)<br />
Trypsin / EDTA Solution<br />
(0.04% / 0.03%)<br />
Trypsin Neutralizing Solution (TNS)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
DetachKit<br />
30 ml<br />
125 ml<br />
250 ml<br />
C-41200<br />
C-41210<br />
C-41220<br />
DetachKit 2<br />
The Promo<strong>Cell</strong> DetachKit 2 was developed<br />
for very gentle detachment of primary<br />
human cells in culture. It contains<br />
less trypsin and EDTA than the standard<br />
DetachKit and is suited for applications,<br />
which require very gentle cell handling.<br />
The DetachKit 2 is optimized for primary<br />
human cells but can also be used for primary<br />
animal cells and cell lines.<br />
The kit contains three components:<br />
HepesBSS (30 mM Hepes)<br />
Trypsin / EDTA Solution<br />
(0.025% / 0.01%)<br />
Trypsin Neutralizing Solution (TNS)<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
DetachKit 2<br />
30 ml<br />
125 ml<br />
250 ml<br />
C-41202<br />
C-41212<br />
C-41222<br />
Accutase-Solution<br />
Accutase is an alternative cell detachment<br />
solution to trypsin and can also<br />
face proteins and epitopes stay entirely<br />
intact. Accutase is therefore perfectly<br />
6 Reagents and<br />
Supplements<br />
Recommended for<br />
be used for tissue dissociation. It was<br />
suited for applications, which require an<br />
developed for very gentle and effective<br />
unchanged cell surface.<br />
Primary cells<br />
detachment of adherent cells. The well<br />
Each batch of the Promo<strong>Cell</strong> Accutase-<br />
<strong>Cell</strong> lines<br />
balanced combination of proteolytic and<br />
Solution is tested on primary human<br />
Tissue dissociation<br />
collagenolytic activities ensures that sur-<br />
cells.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Accutase-Solution,<br />
primary human cell culture tested<br />
100 ml C-41310
102<br />
6 Reagents and Supplements<br />
<strong>Cell</strong> Dissociation Solution ACF<br />
<strong>Cell</strong> Dissociation Solution ACF is a proprietary<br />
non-enzymatic reagent for detachment<br />
of adherent cell types from<br />
tissue culture plastic or glass surfaces.<br />
Due to its gentle yet effective action it<br />
presents a good animal component-free<br />
alternative to trypsin and other enzymatic<br />
detachment solutions.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
<strong>Cell</strong> Dissociation Solution ACF 100 ml C-41320<br />
new<br />
Trypsin Solutions<br />
Trypsin is still the most widely employed<br />
enzyme used for cell detachment.<br />
Promo<strong>Cell</strong>´s Trypsin Solutions exhibit<br />
standardized enzyme activity and contain<br />
trypsin from extra-pure lots.<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Trypsin / EDTA (0.04% / 0.03%)<br />
Trypsin / EDTA 2 (0.025% / 0.01%)<br />
30 ml<br />
125 ml<br />
250 ml<br />
30 ml<br />
125 ml<br />
250 ml<br />
C-41000<br />
C-41010<br />
C-41020<br />
C-41002<br />
C-41012<br />
C-41022<br />
Trypsin PBS 1:250 (0.25%), w/o Ca ++ /Mg ++ 100 ml C-41040<br />
Trypsin PBS 1:250 (2.5%), w/o Ca ++ /Mg ++ ,<br />
100 ml C-41050<br />
10x conc.<br />
Trypsin-EDTA PBS 1:250 (0.05% / 0.02%) 100 ml C-41060<br />
Trypsin-EDTA PBS 1:250, (0.5% / 0.2%),<br />
w/o Ca ++ /Mg ++ , 10x conc.<br />
100 ml C-41070<br />
6 Reagents and<br />
Supplements<br />
Trypsin Neutralizing Solution<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Trypsin Neutralizing Solution (TNS)<br />
(0.05% Trypsin Inhibitor in 0.1% BSA)<br />
30 ml<br />
125 ml<br />
250 ml<br />
C-41100<br />
C-41110<br />
C-41120
6 Reagents and Supplements 103<br />
6.2 Antibiotics and Antimycotics<br />
Antibiotics are used in order to prevent<br />
microbial contaminations in cell culture<br />
during initial isolation steps or to cure<br />
precious cultures exhibiting microbial<br />
contamination, e.g. with mycoplasma or<br />
yeast/fungi.<br />
However, antibiotics may not only harm<br />
contaminating organisms, but can also<br />
unfavorably alter the physiology and<br />
lifespan of primary human cells. Therefore<br />
Promo<strong>Cell</strong> does not recommend<br />
the routine use of antibiotics in cell<br />
culture as a general preventive measure.<br />
As a further application antibiotics may<br />
be utilized in genetic selection procedures,<br />
e.g. G-418.<br />
• Antibiotics and Antimycotics<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Pen/Strep Solution<br />
(10,000 IU/ml / 10 mg/ml)<br />
Pen/Strep/Fungizone Solution<br />
(10,000 IU/ml / 10,000 μg/ml / 25 μg/ml)<br />
100 ml C-42010<br />
100 ml C-42020<br />
Amphotericin B Solution (0.25 mg/ml) 100 ml C-42040<br />
Gentamicin Solution (5 mg/ml) 100 ml C-42060<br />
• Selection Antibiotics<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
G-418 Sulphate Solution (50 mg/ml) 20 ml C-42094<br />
Hygromycin B Solution (50 mg/ml) 20 ml C-42095<br />
6 Reagents and<br />
Supplements
104<br />
6 Reagents and Supplements<br />
6.3 Buffers and Salt Solutions<br />
Buffers and Salt Solutions are frequently<br />
used in tissue culture, cell isolation procedures,<br />
and molecular biology.<br />
For convenience, Promo<strong>Cell</strong> offers<br />
ready-to-use Buffers and Salt Solutions.<br />
They are prepared from raw materials of<br />
the highest quality using tissue culture<br />
grade water.<br />
• Dulbecco´s Phosphate Buffered Saline<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Dulbecco’s PBS 500 ml C-40230<br />
Dulbecco’s PBS, w/o Ca ++ /Mg ++ 500 ml C-40232<br />
Dulbecco’s PBS, w/o Ca ++ /Mg ++ , 10x conc. 500 ml C-40238<br />
• Hanks´ Balanced Salt Solutions<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Hanks’ BSS 500 ml C-40310<br />
Hanks’ BSS, w/o Ca ++ /Mg ++ 500 ml C-40320<br />
Hanks’ BSS, phenol red-free 500 ml C-40370<br />
Hanks’ BSS, w/o Ca ++ /Mg ++ ,<br />
phenol red-free<br />
500 ml C-40390<br />
• Endotoxin-free Buffers and Water<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
PBS, endotoxin-free 500 ml C-40240<br />
PBS, w/o Ca ++ /Mg ++ , endotoxin-free 500 ml C-40242<br />
TC Water 500 ml C-49998<br />
• Other Solutions<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
6 Reagents and<br />
Supplements<br />
CaCl 2<br />
-Solution (0.5 M) 2 ml C-34006<br />
HEPES Buffered Saline (30 mM HEPES)<br />
30 ml<br />
125 ml<br />
250 ml<br />
C-40000<br />
C-40010<br />
C-40020<br />
HEPES Buffer (1 M) 100 ml C-40030<br />
Sodium bicarbonate Solution (7.5%) 100 ml C-42340
6 Reagents and Supplements 105<br />
6.4 Supplements<br />
• Growth Supplements<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
Endothelial <strong>Cell</strong> Growth Supplement*<br />
(bovine hypothalamic extract)<br />
Endothelial <strong>Cell</strong> Growth Supplement/<br />
Heparin* (bovine hypothalamic extract)<br />
Bovine Pituitary Extract 15*<br />
(7.5 mg protein/ml)<br />
Bovine Pituitary Extract 26*<br />
(13 mg protein/ml)<br />
Human Insulin,<br />
primary human cell culture tested<br />
2 ml<br />
10 x 2 ml<br />
2 ml<br />
10 x 2 ml<br />
2 ml<br />
10 x 2 ml<br />
2 ml<br />
10 x 2 ml<br />
C-30160<br />
C-30180<br />
C-30120<br />
C-30140<br />
C-30020<br />
C-30040<br />
C-30021<br />
C-30041<br />
50 mg C-52310<br />
Human Transferrin APO, low iron content 1 g C-52322<br />
Human Transferrin HOLO, iron saturated 1 g C-52332<br />
• Amino Acid Solutions<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
L-Glutamine Solution 100 ml C-42210<br />
Stable Glutamine Solution 100 ml C-42215<br />
MEM Amino Acids Solution 100 ml C-42320<br />
MEM NEAA Solution 100 ml C-42322<br />
• Vitamin and Trace Element Solutions<br />
https://www.promocell.com/shop<br />
Product Size Catalog Number<br />
MEM Vitamin Solution 100 ml C-42328<br />
Trace Element Mix (1000x) 100 ml C-42331<br />
• Extracellular Matrix Proteins<br />
Product Size Catalog Number<br />
Collagen Type I, Rat Tail (3-4 mg/ml)<br />
25 mg<br />
100 mg<br />
https://www.promocell.com/shop<br />
C-43010<br />
C-43020<br />
Fibronectin Solution, bovine (1 mg/ml) 5 ml C-43050<br />
Fibronectin Solution, human (1 mg/ml) 5 ml new C-43060<br />
6 Reagents and<br />
Supplements<br />
* Endothelial <strong>Cell</strong> Growth Supplement (ECGS) and Bovine Pituitary Extract (BPE) are popular growth supplements in primary cell culture. Promo<strong>Cell</strong> ECGS<br />
and BPE are manufactured from raw material which is exclusively collected in New Zealand to ensure maximum BSE-safety.
106<br />
6 Reagents and Supplements<br />
6.5 Cytokines and Growth Factors<br />
Under the brand PromoKine, Promo<strong>Cell</strong><br />
provides a wide range of human, mouse,<br />
and rat cytokines, chemokines, growth<br />
factors, soluble receptors, and CD antigens<br />
(> 500 items). These recombinant<br />
proteins have been produced in E. coli,<br />
yeast, insect, mammalian, or plant cells.<br />
They are highly purified and biologically<br />
active and also available in bulk sizes. For<br />
a complete list of available cytokines and<br />
growth factors please see the PromoKine<br />
catalog or www.promokine.info.<br />
Cytokines mentioned in this catalog:<br />
https://www.promokine.info/shop<br />
Product Size Catalog Number<br />
EGF, human, recombinant (E. coli) 500 μg C-60170<br />
EGF, human, recombinant (Pichia) 500 μg C-60182<br />
FGF-1 (FGF-a), human, recombinant<br />
(E. coli)<br />
FGF-2 (FGF-b), human, recombinant<br />
(E. coli)<br />
50 μg C-60340<br />
50 μg C-60240<br />
flt-3 Ligand, human, recombinant (E. coli) 10 μg C-67110<br />
G-CSF, human, recombinant (E. coli) 10 μg C-60434<br />
GM-CSF, human, recombinant (E. coli) 10 μg C-60420<br />
IL-3 CC, human, recombinant (E. coli) 30 μg C-61301<br />
IL-4 CC, human, recombinant (E. coli) 5 μg C-61401<br />
IL-6 CC, human, recombinant (E. coli) 10 μg C-61601<br />
Insulin, human, recombinant (E. coli) 25 mg C-60212<br />
M-CSF, human, recombinant (E. coli) 10 μg C-60442<br />
SCF, human, recombinant (E. coli) 10 μg C-63120<br />
sRANKL, human, recombinant (E. coli) 10 μg C-63200<br />
6 Reagents and<br />
Supplements<br />
TNF-alpha, human, recombinant (E. coli) 10 μg C-63722<br />
TPO, human, recombinant (E. coli) 10 μg C-65112<br />
VEGF-165, human, recombinant (E. coli) 10 µg C-64420<br />
VEGF-165, human, recombinant (HEK293) 10 µg C-64423
Microbial Detection<br />
7<br />
and Elimination<br />
<strong>Cell</strong> culture contamination is a common problem that impacts<br />
many biochemical and immunological properties of cells and it<br />
leads to unsatisfactory and unreliable results. Therefore, regular<br />
testing of cell cultures for mycoplasmas/bacteria contamination<br />
is essential.<br />
Promo<strong>Cell</strong> provides highly sensitive and user-friendly test kits<br />
for this purpose, as well as products for the effective elimination<br />
and prevention of mycoplasma and eubacteria contamination.
108<br />
Content Chapter 7<br />
7<br />
Microbial Detection<br />
and Elimination<br />
7.1 Mycoplasma Test Kits ............... 109<br />
7.2 Mycoplasma Elimination Solutions. ..... 110<br />
7.3 Bacteria Test Kit. ................... 112<br />
7.4 Disinfectants ...................... 113
7 Microbial Detection and Elimination 109<br />
7.1 Mycoplasma Test Kits<br />
Applications<br />
Mycoplasma detection in cell culture,<br />
virus stocks, and medium<br />
Conventional and real-time PCR<br />
Features<br />
Highly sensitive (detects 10 - 15<br />
mycoplasmas per sample volume)<br />
Fast, reproducible, and reliable results<br />
Applicable for all cell/tissue culturerelevant<br />
mycoplasma species<br />
Positive and internal controls included<br />
Convenient protocol and easy sample<br />
preparation<br />
Mycoplasmas are parasitic bacteria that<br />
frequently contaminate tissue cultures<br />
and consequently also virus stocks, vaccines,<br />
and other biological materials produced<br />
in cells. The contamination can<br />
originate from serum, laboratory staff,<br />
other contaminated cultures, or the donors<br />
from which the cells have been harvested,<br />
and it often remains unnoticed.<br />
Although mycoplasmas do not cause<br />
visible cell damage, they seriously affect<br />
cell growth and metabolism, transfection<br />
efficiency, protein and monoclonal<br />
antibody synthesis, cytokine secretion,<br />
immunological properties, signal transduction,<br />
virus proliferation and can even<br />
cause DNA and RNA damage.<br />
Since mycoplasmas are very small and<br />
lack cell walls, detection using a conventional<br />
light microscope is nearly<br />
impossible. In addition, cloudiness in<br />
the medium, typical for bacterial or<br />
yeast contaminations, does not occur<br />
even at high mycoplasma concentrations.<br />
PCR on the other hand is a highly<br />
sensitive, specific, and rapid method<br />
for the detection of mycoplasma contamination<br />
in culture supernatant, cell/<br />
virus stocks, and medium. All cell/tissue<br />
culture relevant mycoplasma species can<br />
be detected in one assay including M.<br />
fermentans, M. hyorhinis, M. arginini,<br />
M. orale, M. salivarium, M. hominis,<br />
M. pulmonis, and M. pirum. In addition,<br />
Acholeplasma laidlawii, M. synoviae,<br />
and Ureaplasma species can be<br />
detected.<br />
PCR Mycoplasma Test Kit I/C<br />
The PCR Mycoplasma Test Kit I/C utilizes<br />
conventional PCR technology for<br />
convenient and specific detection of<br />
more than 25 contaminating mycoplasma<br />
species. The primer set is specific<br />
for the highly conserved 16S-rRNA coding<br />
region in the mycoplasma genome.<br />
The test can be performed in 3 hours<br />
and includes internal control DNA (for<br />
verifying a successful PCR run) as well<br />
as positive control DNA. All components<br />
required for the PCR (primers, nucleotides,<br />
internal control DNA) come optimally<br />
premixed and lyophilized in PCR<br />
tubes (except the Taq polymerase). For<br />
setting up the PCR, simply cut off the<br />
number of tubes required, rehydrate<br />
with rehydration buffer (containing the<br />
Hot-Start Taq polymerase), and add the<br />
sample to be analyzed. Samples can be<br />
easily prepared by boiling the cell culture<br />
supernatant or other sample material.<br />
PCR Mycoplasma Test Kit I/RT<br />
The PCR Mycoplasma Test Kit I/RT utilizes<br />
real-time PCR technology for convenient<br />
and reproducible detection of<br />
contaminating mycoplasmas. It shows<br />
excellent sensitivity and specificity and<br />
enables reliable, quantitative PCR results<br />
quickly (making the kit particularly<br />
useful for routine testing). The primer<br />
and probe set is specific for the highly<br />
conserved 16S-rRNA coding region in<br />
the mycoplasma genome. It contains<br />
FRET probes emitting fluorescent light<br />
at 520 nm and is compatible with most<br />
common Real-Time PCR cyclers (e.g.<br />
Roche Light Cycler ® 1 and 2, ABI Prism ® ,<br />
Cepheid Smart Cycler ® , and Eppendorf<br />
Mastercycler ® ep realplex).<br />
The test includes internal control DNA<br />
(for verifying a successful PCR run) as<br />
well as positive control DNA. All components<br />
required for real time PCR<br />
(primers, probes, nucleotides, and internal<br />
control DNA) come lyophilized<br />
in PCR tubes, except the Hot-Start Taq<br />
polymerase which is dissolved in reaction<br />
buffer. Two kit variants (kit A for<br />
e.g. Light Cycler, kit B for e.g. ABI Prism)<br />
are provided which have been optimized<br />
for different Real Time PCR instruments.<br />
For recommended kit variant, please see<br />
www.promokine.info where you can<br />
download the manual.<br />
PCR<br />
100 µl cell culture<br />
supernatant<br />
Boil for 10 minutes<br />
and centrifuge briefly<br />
2 µl supernatant<br />
Analyze 5 µl of<br />
the PCR sample<br />
7 Microbial<br />
Detection and<br />
Elimination<br />
Fig. 1: Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient: Boil 100 µl cell culture<br />
supernatant or other sample material for 10 minutes; centrifuge briefly and add 2 µl supernatant to the reaction cup with the<br />
rehydrated PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis.
110<br />
7 Microbial Detection and Elimination<br />
PCR Mycoplasma Test Kit III<br />
The PCR Mycoplasma Test Kit III utilizes<br />
conventional PCR technology for convenient<br />
and highly sensitive detection of<br />
mycoplasma. The primer set is specific for<br />
the highly conserved 16S-rRNA coding<br />
region in the mycoplasma genome. Thus,<br />
the kit reliably detects all mycoplasma<br />
commonly found in cell culture, including<br />
the Acholeplasma and Spiroplasma<br />
genera. The kit has been positively tested<br />
against all mycoplasma strains listed in the<br />
European Pharmacopoeia, but may detect<br />
even more mycoplasma species (~70-<br />
120 species). It does not detect microorganisms<br />
such as Streptococcus sp.,<br />
Clostridium sp. and Lactobacillus sp.<br />
which are phylogenetically closely related.<br />
The kit includes all the components required<br />
for PCR (nucleotides, primers, a<br />
high-performance Taq polymerase, and<br />
an agarose gel loading dye), all premixed<br />
at optimal concentrations for the preparation<br />
of a ready-to-use and ready-toload<br />
1x master mix. The test takes approximately<br />
3 hours to run and obtain<br />
clear results. The kit also contains positive<br />
control DNA for easy result verification as<br />
well as an internal control DNA to verify a<br />
successful PCR run.<br />
M 1 2 3 4 5 6<br />
1 2 3 4 5 6 7 M<br />
479 bp<br />
270 bp<br />
260 bp<br />
160 bp<br />
Fig. 2 Top: Detection of mycoplasmas<br />
with PCR Mycoplasma Test Kit I/C.<br />
M: 100 bp DNA ladder<br />
1: Positive control<br />
2-6: 10; 100; 1,000; 10,000; 100,000<br />
mycoplasmas/sample volume<br />
Internal control DNA: 479 bp band<br />
Positive sample/control: 270 bp band<br />
Bottom: Detection of mycoplasmas<br />
with PCR Mycoplasma Test Kit III.<br />
1-4: A. laidlawii, S. citri, M. synoviae,<br />
and M. orale (50 cfu/ml each)<br />
5: <strong>Cell</strong> culture supernatant, not infected<br />
6: Negative control<br />
7: Positive control<br />
M: DNA size standard<br />
Internal control DNA: 160 bp band<br />
Positive sample/control: 260-270 bp band<br />
https://www.promokine.info/shop<br />
Product Size Catalog Number<br />
PCR Mycoplasma Test Kit I/C<br />
24 tests<br />
48 tests<br />
96 tests<br />
PK-CA91-1024<br />
PK-CA91-1048<br />
PK-CA91-1096<br />
PCR Mycoplasma Test Kit I/RT; Variant A 25 tests PK-CA91-3025A<br />
PCR Mycoplasma Test Kit I/RT; Variant B 25 tests PK-CA91-3025B<br />
PCR Mycoplasma Test Kit III 100 tests PK-CA92-100<br />
RTU DNA Ladder I (100 bp) 0.5 ml PK-MB713-001N<br />
RTU DNA Ladder II (1000 bp) 0.5 ml PK-MB713-002N<br />
Please see Chapter 1.11 of the PromoKine Catalog for the ready-to-use DNA Ladders.<br />
7 Microbial<br />
Detection and<br />
Elimination<br />
7.2 Mycoplasma Elimination Solutions<br />
Mycoplasma contamination seriously affects<br />
cell growth and cell physiology leading<br />
to biased and unreliable experimental<br />
results. When mycoplasma contamination<br />
is detected, the contaminated cells<br />
are ideally discarded and all equipment,<br />
e.g. work benches, CO 2<br />
incubators, etc.,<br />
are disinfected (see chapter 7.4 for disinfectant<br />
sprays). However, if the affected<br />
cells are unique or the result of<br />
extensive work, thorough elimination of<br />
the contaminating mycoplasmas is necessary.<br />
Standard antibiotics commonly<br />
used in cell culture (e.g. Pen/Strep) do<br />
not affect mycoplasmas. Therefore,<br />
PromoKine offers two highly effective<br />
methods to get rid of mycoplasma contamination:<br />
Mycoplasma-EX, a combination<br />
of non-antibiotic and antibiotic<br />
reagents, and BIOMYC 1-3, three highly<br />
effective antibiotics.
7 Microbial Detection and Elimination 111<br />
Mycoplasma-EX<br />
Applications<br />
Mycoplasma elimination in cell/tissue<br />
culture<br />
Mycoplasma elimination in virus<br />
stocks<br />
Features<br />
Highly effective against mycoplasma<br />
species commonly found in cell<br />
cultures<br />
Very short treatment time<br />
Very low cytotoxicity<br />
Ready to use<br />
Easy handling<br />
Mycoplasma-EX combines a non-antibiotic<br />
and an optimized antibiotic treatment<br />
for the effective elimination of<br />
mycoplasmas and other microorganisms<br />
from cell/tissue culture and virus stocks.<br />
It eliminates mycoplasmas by directly<br />
killing them and not just by inhibiting<br />
their growth, with minimal toxicity for<br />
the cells in culture.<br />
Mycoplasma-EX is effective after a single<br />
treatment, and destroys mycoplasmas<br />
rapidly and permanently. It is applicable<br />
for most cell lines (e.g. Vero, BHK21,<br />
GBK, ML, Hep2, 293, CRFK, H9, Molt4,<br />
MT-4, Jurkat), as well as virus stocks<br />
(e.g. SHV-1, BHV-1, HSV-1, VSV, SFV,<br />
FCV, MEV) and is highly effective against<br />
M. orale, M. hyorhinis, M. hominis,<br />
Acholeplasma laidlawii, M. arginini, M.<br />
fermentans, and other mycoplasma species<br />
encountered as contaminants in cell<br />
cultures.<br />
BIOMYC Antibiotic<br />
Solutions<br />
Applications<br />
Mycoplasma elimination in cell/<br />
tissue culture<br />
Features<br />
Highly effective against mycoplasma<br />
species commonly found in cell<br />
cultures<br />
Low cytotoxicity<br />
Ready to use<br />
Easy handling<br />
Antibiotics that impact bacterial cell walls<br />
(e.g. penicillin) are ineffective against<br />
mycoplasmas, which lack a cell wall.<br />
Other antibiotics such as tylosin, neomycin,<br />
tetracycline, or gentamicin are<br />
only effective against a few mycoplasma<br />
species commonly found in cell cultures.<br />
In addition, they frequently just reduce<br />
the concentration of mycoplasmas rather<br />
than completely eliminating them. As<br />
soon as treatment is discontinued, contamination<br />
will recur.<br />
BIOMYC-1 and 2<br />
BIOMYC-1 and 2 are two antibiotics,<br />
which eliminate mycoplasmas very efficiently<br />
when used in combination. After<br />
a four-day treatment with BIOMYC-1,<br />
a three-day treatment with BIOMYC-2<br />
follows. This is repeated 2 to 3 times.<br />
Following this protocol, mycoplasmas<br />
will not develop resistance to these antibiotics<br />
- a common occurrence with<br />
other antibiotic treatment methods.<br />
BIOMYC-1 is based on the antibiotic tiamutin<br />
which is produced by the fungus<br />
Pleurotus mutilus. BIOMYC-2 is based<br />
on the antibiotic minocycline, an improved<br />
derivative of tetracycline.<br />
BIOMYC-3<br />
BIOMYC-3 is based on the antibiotic<br />
ciprofloxacin, which is a member of<br />
the fluoroquinolone group and inhibits<br />
synthesis of DNA gyrase, an enzyme<br />
necessary for bacterial DNA supercoiling.<br />
Many mycoplasma species are<br />
sensitive to BIOMYC-3, including M.<br />
orale, M. hyorhinis, M. fermentans, and<br />
M. arginini, and also Acheloplasma laidlawii.<br />
These species are responsible for<br />
most mycoplasma contaminations in cell<br />
cultures. No cytotoxic effects on the cells<br />
in culture have been found at the concentrations<br />
recommended for use, and<br />
the treatment can be performed within<br />
12 days.<br />
Fig. 3: Fluorescence microscope image of<br />
cells contaminated with mycoplasmas.<br />
Picture kindly provided by M. Ebert,<br />
Forschungszentrum für Medizintechnik<br />
und Biotechnologie, Erfurt, Germany.<br />
https://www.promokine.info/shop<br />
Product Size Catalog Number<br />
Mycoplasma-EX 3 treatments PK-CC91-4003<br />
BIOMYC-1<br />
BIOMYC-2<br />
BIOMYC-3<br />
10 ml<br />
20 ml<br />
100 ml<br />
10 ml<br />
20 ml<br />
100 ml<br />
10 ml<br />
20 ml<br />
100 ml<br />
PK-CC03-036-1D<br />
PK-CC03-036-1C<br />
PK-CC03-036-1B<br />
PK-CC03-037-1D<br />
PK-CC03-037-1C<br />
PK-CC03-037-1B<br />
PK-CC03-038-1D<br />
PK-CC03-038-1C<br />
PK-CC03-038-1B<br />
7 Microbial<br />
Detection and<br />
Elimination
112<br />
7 Microbial Detection and Elimination<br />
7.3 Bacteria Test Kit<br />
Applications<br />
Bacteria detection in cell culture<br />
Bacteria detection in virus stocks<br />
Bacteria detection in medium<br />
Features<br />
Highly sensitive (detects 12 bacteria<br />
genomes per sample volume)<br />
Reproducible and reliable results<br />
Applicable for 45 bacteria species<br />
Positive and internal controls included<br />
Convenient protocol and easy sample<br />
preparation<br />
Bacteria are frequently found as contaminants<br />
in cell cultures. However, cell<br />
cultures sometimes lack visual signs of<br />
the bacterial contamination, such as fast<br />
decoloration of the medium. In addition,<br />
it has been demonstrated that standard<br />
antibiotics are mostly ineffective against<br />
resistant bacterial infection but have<br />
a strong impact on the metabolism,<br />
growth, and differentiation of the cells<br />
in culture.<br />
Frequent screening of cell cultures for<br />
bacterial contamination is essential<br />
to ensure reliable results. Therefore,<br />
PromoKine offers a PCR-based Bacteria<br />
Test Kit that has been developed for<br />
accurate and reliable in vitro diagnosis of<br />
45 bacteria species, which are the common<br />
contaminants in cell cultures (e.g.<br />
Actinomyces, Bacillus, Enterococcus,<br />
Escherichia coli, Fusobacterium, Klebsiella,<br />
Lactobacillus, Micrococcus, Mycobacterium,<br />
Peptostreptococcus, Pseudomonas,<br />
Porphyromonas, Prevotella,<br />
Salmonella, Serratia, Staphylococcus,<br />
Streptococcus, etc.). The kit provides a<br />
highly sensitive, easy, and rapid method<br />
to detect bacterial infection in various<br />
biological materials, including cell cultures.<br />
Detection requires as little as 12<br />
bacteria genomes (~52 fg) per sample<br />
volume. The protocol can be completed<br />
within 3.5 hours with a hands-on time<br />
of less than 40 minutes. The kit contains<br />
all the necessary components for setting<br />
up a PCR including a positive control<br />
DNA as well as an internal control DNA<br />
to confirm a successfully performed PCR<br />
run. The kit does not amplify eukaryotic<br />
DNA.<br />
PCR<br />
200 - 500 µl cell<br />
culture supernatant<br />
Extract Bacterial<br />
DNA<br />
5 µl DNA sample<br />
Analyze 5 µl of<br />
the PCR sample<br />
Fig. 4: Bacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient: Purify bacterial DNA from 200 – 500<br />
µl of cell culture supernatant using an appropriate DNA extraction kit; add 5 µl sample DNA to the reaction cup with the premixed<br />
PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis.<br />
https://www.promokine.info/shop<br />
Product Size Catalog Number<br />
PCR Bacteria Test Kit 24 tests PK-CA91-2024<br />
PCR Bacteria Test Kit 48 tests PK-CA91-2048<br />
PromoKine also offers a Bacteria Live/Dead Staining Kit as well as fluorescent dyes for staining of bacteria, please see Chapter 1<br />
of the PromoKine Catalog.<br />
7 Microbial<br />
Detection and<br />
Elimination
7 Microbial Detection and Elimination 113<br />
7.4 Disinfectants<br />
Disinfection of all tissue culture equipment<br />
and work surfaces in the lab has to<br />
be performed regularly to prevent contamination.<br />
PromoKine offers different<br />
disinfectants ideal for laboratory surfaces<br />
and apparatuses, CO 2<br />
incubators, and<br />
water baths.<br />
For more information please see<br />
www.promocell.com/knowledge-base<br />
Mycoplasma-EX Spray<br />
Applications<br />
Disinfection of all laboratory surfaces<br />
Disinfection of laboratory apparatuses<br />
Features<br />
Kills mycoplasmas within seconds<br />
Effective against most microorganisms<br />
Non-corrosive and non-cancerogenic<br />
Does not leave any residue<br />
Supplied in a refillable spray bottle<br />
cidal activity, it is very effective against<br />
Staphylococcus aureus, Streptococcus<br />
faecalis, Escherichia coli, Proteus mirabilis,<br />
Pseudomonas aeruginosa, Candida<br />
albicans, Mycobacteria, HBV/HIV, Papova,<br />
Adeno, Polio and Vaccinia viruses.<br />
The effectiveness of Mycoplasma-EX<br />
Spray is further enhanced by its excellent<br />
detergent properties, achieving clean and<br />
contamination-free surfaces in one step.<br />
Pharmacidal Spray<br />
Applications<br />
Disinfection of incubators<br />
Features<br />
Free of mercury, formaldehyde,<br />
phenol, and alcohol<br />
Very effective against bacteria, fungi,<br />
spores, and viruses<br />
Non-corrosive and non-cancerogenic<br />
Does not leave any residue<br />
Supplied in a refillable spray bottle<br />
Pharmacidal Spray is a mercury-, formaldehyde-,<br />
phenol-, and alcohol-free<br />
disinfectant specifically designed for the<br />
disinfection of incubators. Spraying the<br />
incubators thoroughly every two weeks<br />
is recommended.<br />
Aquaguard<br />
Applications<br />
Disinfection of water trays in CO 2<br />
incubators<br />
Disinfection of water baths<br />
Features<br />
Very effective against bacteria, fungi,<br />
spores, and viruses<br />
Non-corrosive<br />
Non-toxic and non-volatile<br />
The water in an incubator or water bath<br />
is a major source of cell culture contamination,<br />
particularly with respect<br />
to mycoplasma contaminations, since<br />
mycoplasmas can survive in pure water.<br />
Aquaguard, a non-volatile disinfectant,<br />
is extremely effective at eliminating the<br />
microorganisms in water. In addition, it<br />
does not cause damage to the stainless<br />
steel of the incubator or any plastic interior,<br />
and it does not cause any skin irritation<br />
when used as recommended.<br />
Aquaguard-1 is intended for disinfecting<br />
water in incubators, whereas Aquaguard-2<br />
is intended for water bath disinfection.<br />
Mycoplasma-EX Spray allows safe disinfection<br />
and cleaning of all laboratory surfaces<br />
and apparatuses, including work<br />
benches, incubators, cell storage boxes,<br />
and liquid nitrogen containers. It utilizes<br />
the powerful Mycoplasma-EX reagent<br />
in combination with an alcohol-based<br />
solution. Besides its strong mycoplasmahttps://www.promokine.info/shop<br />
Product Size Catalog Number<br />
Mycoplasma-EX Spray<br />
Pharmacidal Spray<br />
Aquaguard-1 (incubator)<br />
Aquaguard-2 (water bath)<br />
Mycoplasma Disinfection Kit I<br />
(Consists of Mycoplasma-Ex Spray 500 ml,<br />
Aquaguard-1, and Aquaguard-2)<br />
Mycoplasma Disinfection Kit II<br />
(Consists of Pharmacidal Spray 1 L,<br />
Aquaguard-1, and Aquaguard-2)<br />
500 ml<br />
5 L<br />
1000 ml<br />
5 L<br />
100 ml<br />
10 x 100 ml<br />
50 ml<br />
10 x 50 ml<br />
PK-CC91-5050<br />
PK-CC91-5050-5L<br />
PK-CC-110100<br />
PK-CC-110100-5L<br />
PK-CC01-867-1B<br />
PK-CC01-867-10B<br />
PK-CC01-916-1E<br />
PK-CC01-916-10E<br />
1 Kit PK-CC91-5060<br />
1 Kit PK-CC91-5070<br />
7 Microbial<br />
Detection and<br />
Elimination
PromoKine<br />
8<br />
<strong>Cell</strong> Biology Products<br />
PromoKine – a brand of Promo<strong>Cell</strong> - provides a wide range of<br />
state-of-the-art tools for cell biology research including many<br />
innovative reagents and kits for cell analysis, cell transfection,<br />
fluorescent labeling, gene cloning and expression, and mycoplasma<br />
detection and elimination. Also PromoKine offers<br />
a huge variety of recombinant proteins such as cytokines,<br />
growth factors, caspases, and kinases as well as ELISA kits,<br />
and primary and secondary antibodies.
116<br />
Content Chapter 8<br />
3<br />
8<br />
PromoKine - <strong>Cell</strong> Biology Products<br />
8.1 <strong>Cell</strong> Analysis ...................... 117<br />
8.2 Apoptosis ........................ 117<br />
8.3 Fluorescent Labeling ................ 117<br />
8.4 Gene Cloning, Expression, and Silencing . 118<br />
8.5 <strong>Cell</strong> Transfection ................... 118<br />
8.6 Antibodies and ELISAs. .............. 118<br />
8.7 Cytokines and Growth Factors. ........ 119
8 <strong>Cell</strong> Biology 117<br />
8.1 <strong>Cell</strong> Analysis<br />
8.2 Apoptosis 8.3 Fluorescent Labeling<br />
The specific analysis of cellular processes<br />
and conditions has played an increasingly<br />
vital role in cell biology. Monitoring<br />
reporter gene expression in cells, determining<br />
cell viability and proliferation,<br />
testing for cytotoxicity, and distinguishing<br />
between live and dead cells are important<br />
procedures in most cell biology<br />
labs. Measuring intracellular signaling<br />
and assaying distinct cell metabolic activities<br />
have also become routine. These<br />
applications all require reliable, sophisticated,<br />
and user-friendly research tools.<br />
Thus, PromoKine offers numerous reagents<br />
and kits for cell analysis, e.g. for<br />
detection and quantitation of cellular<br />
processes or staining of cells or cell structures:<br />
<strong>Cell</strong> Viability and Proliferation Kits<br />
Live/Dead <strong>Cell</strong> Staining Kits<br />
Cytotoxicity Kits<br />
Senescence Detection Kit<br />
<strong>Cell</strong>-Based Reporter Assays<br />
<strong>Cell</strong> Staining Reagents and Related<br />
Products<br />
Intracellular Indicators and Related<br />
Products<br />
<strong>Cell</strong> Signaling Assays<br />
<strong>Cell</strong> Stress and Metabolism Kits<br />
<strong>Cell</strong> Fractionation and Protein<br />
Extraction Kits<br />
Nucleic Acid Isolation Kits<br />
Apoptosis, or programmed cell death, is<br />
a sophisticated process that involves an<br />
intricate series of biochemical events.<br />
Specific cell death signals are transduced<br />
by different biomolecules such<br />
as caspases, which initiate an apoptotic<br />
cascade. This process culminates in multiple<br />
events such as degradation of cellular<br />
proteins and chromosomal DNA,<br />
mitochondrial disruption, and changes in<br />
plasma membrane integrity.<br />
For the reliable detection and quantification<br />
of different apoptotic events such<br />
as DNA fragmentation, mitochondria<br />
and plasma membrane changes as well<br />
as enzymatic activities of apoptosisspecific<br />
proteins, PromoKine provides<br />
an extensive line of user-friendly kits<br />
and reagents for highly sensitive colorimetric<br />
or fluorometric measurement:<br />
DNA Fragmentation Detection Kits<br />
Mitochondrial Apoptosis Staining Kit<br />
Cytochrome c Apoptosis Detection Kit<br />
Glutathione Detection Kits<br />
Annexin V Detection Kits<br />
Caspase Detection Kits<br />
Caspase Quantification Kits<br />
Cathepsin/Calpain Detection Kits<br />
Kinase Detection Kits<br />
Apoptotic and Necrotic <strong>Cell</strong> Detection<br />
Apoptotic <strong>Cell</strong> Isolation Kit<br />
Apoptosis Inducers<br />
The availability of new fluorophores has<br />
dramatically changed the possibilities for<br />
the sensitive detection of biomolecules<br />
and the analysis of their interactions.<br />
Improved fluorescent dyes are now enabling<br />
previously impossible studies of<br />
cellular structures and processes.<br />
Our PromoFluor dyes are cost-effective,<br />
high quality alternatives to commonly<br />
used, well-known fluorophores and span<br />
the entire visible wavelengths spectrum<br />
from blue to far red.<br />
PromoKine offers a wide choice of reactive<br />
fluorescent dyes for easy labeling of<br />
diverse biomolecules including proteins,<br />
antibodies, and DNA, as well as complete<br />
labeling kits and ready-to-use fluorescent<br />
conjugates:<br />
PromoFluor Reactive Dyes<br />
PromoFluor Protein and Antibody<br />
Labeling Kits<br />
PromoFluor DNA Labeling Kits<br />
PromoFluor Labeled Antibodies<br />
PromoFluor Labeled Phalloidin<br />
PromoFluor Labeled Nucleotides<br />
PromoFluor Labeled Biotin<br />
and Streptavidin<br />
Phycobiliprotein Dyes<br />
Phycobiliprotein Labeled Biotin and<br />
Streptavidin<br />
PromoFluor Antifade Reagent<br />
More information can be found in our PromoKine catalog or at the PromoKine website www.promokine.info<br />
Fig. 1: Double-staining of viable<br />
(green) and dead (red) cells using the<br />
PromoKine Live/Dead <strong>Cell</strong> Staining Kit II.<br />
Fig. 2: Apoptotic U937 cells stained with<br />
Annexin V-FITC using the PromoKine<br />
Apoptotic/Necrotic/Healthy <strong>Cell</strong>s Detection<br />
Kit.<br />
Fig. 3: <strong>Cell</strong> structures of HeLa cells<br />
visualized using a primary anti-betatubulin<br />
antibody and a secondary<br />
PromoFluor 488-labeled antibody (green)<br />
as well as a primary anti-centromere antibody<br />
and a secondary PromoFluor 547-<br />
labeled antibody (red).<br />
8 <strong>Cell</strong> Biology
118<br />
8 <strong>Cell</strong> Biology<br />
8.4 Gene Cloning,<br />
Expression, and Silencing<br />
Manipulating DNA via cloning and PCR<br />
techniques facilitates complex genetic<br />
analysis. PCR has become a core technique<br />
in molecular biology and it enables<br />
a wide array of biological experiments<br />
and practical applications. Amplifying<br />
DNA has also allowed the study of gene<br />
expression, including the examination<br />
of genetic information transcribed and<br />
translated into RNA and proteins. In<br />
contrast, gene silencing has also grown<br />
into an important technique for analyzing<br />
gene functions.<br />
PromoKine provides a selection of vector<br />
systems allowing convenient cloning and<br />
high-level expression of specific target<br />
genes of interest as well as plasmid vectors<br />
expressing commonly used reporter<br />
genes or siRNA templates to generate<br />
specific siRNAs for gene silencing studies:<br />
pPK-CMV Expression Vectors<br />
pPK-CMV Reporter Vectors<br />
pPK-CMV Fusion Vectors<br />
StarGate Combinatorial Cloning<br />
System<br />
StarGate Mutagenesis System<br />
StarGate Fusion Cloning System<br />
GeneOFF shRNA Vector Kits<br />
GeneOFF siRNA Generation Kit<br />
8.5 <strong>Cell</strong> Transfection<br />
The ability to introduce foreign nucleic<br />
acids into eukaryotic cells has become a<br />
powerful tool to study and control gene<br />
expression. Cloned genes can be transfected<br />
into cells for biochemical characterization,<br />
mutational analyses, investigation<br />
of gene regulatory elements, and the<br />
production of specific proteins. <strong>Cell</strong> transfection<br />
promotes the rapid advancement<br />
of our knowledge of genetic regulation<br />
and protein function.<br />
PromoFectin Transfection Reagents provide<br />
highly efficient delivery of nucleic acids<br />
and proteins/peptides into a variety of<br />
adherent and non-adherent cell types, including<br />
many hard-to-transfect cell lines<br />
and primary cells. They show very low<br />
cytotoxicity and are therefore also ideally<br />
suited for transfection of very sensitive<br />
cells. PromoFectin variants optimized for<br />
transfection of HUVECs, hepatocytes,<br />
macrophages, neuronal, and insect cells<br />
are also available.<br />
In addition, the innovative Magnet-Assisted<br />
Transfection technology (MATra)<br />
provides another highly effective means<br />
for a very fast (transfection is completed<br />
within 15 – 20 minutes!) and successful<br />
delivery of nucleic acids into a variety of<br />
mammalian cells – also including many<br />
difficult-to-transfect cell types:<br />
PromoFectin Transfection Reagents<br />
Magnet-Assisted Transfection (MATra)<br />
Transfection Accessories<br />
8.6 Antibodies<br />
and ELISAs<br />
Antibodies have a broad range of applications<br />
like the detection of cellular<br />
structures as well as the specific identification,<br />
localization, and purification of<br />
intracellular and extracellular biomolecules<br />
such as proteins. They are also<br />
essential tools in describing cellular processes<br />
and for diagnostic purposes. Consequently,<br />
they are important for many<br />
research disciplines in cell biology.<br />
PromoKine offers a wide range of antibodies<br />
and ELISAs for different research<br />
fields such as cell signaling/signal transduction,<br />
apoptosis, neuroscience, virology,<br />
homeostasis, stem cell and cancer<br />
research.<br />
In addition, labeled secondary antibodies,<br />
antibody labeling kits as well as a variety<br />
of control cell lysates and blocking<br />
peptides are available:<br />
Mono- and Polyclonal Primary Antibodies<br />
Labeled Primary and Secondary<br />
Antibodies<br />
Antibody Labeling Kits<br />
Western Blotting Kits and Reagents<br />
Ready-to-use ELISA Kits<br />
ELISA Development Kits<br />
Antigens (Blocking Peptides)<br />
<strong>Cell</strong> Lysates<br />
More information can be found in our PromoKine catalog or at the PromoKine website www.promokine.info<br />
Fig. 4: b-Gal staining of CHO-K1 cells<br />
Fig. 5: Primary neurons transfected with<br />
Fig. 6: PromoKine ELISA kits are dis-<br />
8 <strong>Cell</strong> Biology<br />
transfected with the PromoKine pPK-<br />
CMV-R1 reporter vector expressing the<br />
lacZ gene.<br />
the PromoKine reporter vector pPK-<br />
CMV-R4 (see chapter 4 of PromoKine<br />
Catalog) using PromoFectin-Neuron.<br />
tinguished by their high sensitivity and<br />
reproducibility as well as their userfriendly<br />
and convenient protocols.
8 <strong>Cell</strong> Biology 119<br />
8.7 Cytokines<br />
and Growth Factors<br />
Cytokines and chemokines are small proteins<br />
secreted by a variety of cell types.<br />
They are important signal messengers<br />
mediating cell communication and activating<br />
other target cells through their<br />
binding to specific receptors.<br />
Cytokines play a central role in the immune<br />
system - in which they mainly<br />
function as chemoattractants. They also<br />
coordinate and regulate other biological<br />
processes such as cell growth and development,<br />
or tissue repair.<br />
For many years, PromoKine has provided<br />
an exceptionally wide range of highquality<br />
recombinant proteins that were<br />
produced in E. coli, yeast, insect, plant<br />
or mammalian cells. They meet the highest<br />
quality standards with regard to their<br />
stability, purity, and biological activity.<br />
Our recombinant proteins are very useful<br />
for studying cellular processes such<br />
as cell differentiation and programming,<br />
angiogenesis and aging as well as diseases<br />
like obesity, diabetes, and cancer:<br />
Cytokines, Chemokines,<br />
and Growth Factors<br />
Hormones<br />
Animal-free Cytokines and<br />
Growth Factors<br />
<strong>Cell</strong> <strong>Culture</strong>-grade Cytokines<br />
Soluble Receptors and CD Antigens<br />
Cytokines and Growth Factors<br />
for Stem <strong>Cell</strong> Research<br />
Bulk sizes available at very<br />
economical prices<br />
OD (490 nm)<br />
1.6<br />
1.5<br />
Control fraction<br />
PromoKine´s animal-free VEGF-121<br />
Fig. 7: <strong>Cell</strong> proliferation assay using<br />
PromoKine´s animal-free VEGF-121 and HU-<br />
VEC cells (see page 11, Cat.No. C-12200).<br />
1.4<br />
1.3<br />
1.2<br />
0.1<br />
1.0<br />
0.9<br />
0.8<br />
0.7<br />
0.0 10-³ 10-² 10-¹ 1.0 10¹ 10²<br />
ng/ml VEGF-121<br />
10³<br />
More information can be found in our PromoKine catalog or at the PromoKine website www.promokine.info<br />
8 <strong>Cell</strong> Biology
120<br />
Alphabetical Index<br />
Alphabetical Index<br />
Description Page Description Page<br />
1 - 9<br />
3D-Angiogenesis Assay (HDMEC) 60<br />
3D-Angiogenesis Assay (HUVEC) 60<br />
Chondrocytes 10<br />
Chondrogenic Differentiation Medium 86<br />
Classical Media 90<br />
DMEM Media 90<br />
DMEM/Ham´s F-12 Media 90<br />
Accutase-Solution 101<br />
Adipocyte Media 83<br />
Adipogenic Differentiation Medium 86<br />
Airway Epithelial <strong>Cell</strong> Media 71<br />
Amphotericin B Solution 103<br />
Angiogenesis Assays and Kits 59<br />
Antibiotics and Antimycotics 103<br />
Antibodies and ELISAs 118<br />
Aortic Adventitial Fibroblasts 26<br />
Aortic Endothelial <strong>Cell</strong>s 13<br />
Aortic Smooth Muscle <strong>Cell</strong>s 35<br />
Apoptosis 117<br />
Aquaguard 113<br />
Bacteria Test Kit 112<br />
BIOMYC 111<br />
Bladder Microvascular Endothelial <strong>Cell</strong>s 18<br />
Bladder Smooth Muscle <strong>Cell</strong>s 38<br />
Blood <strong>Cell</strong>s 46<br />
Blood Separation Medium 89<br />
Bovine Pituitary Extract 105<br />
Brain Microvascular Endothelial <strong>Cell</strong>s 20<br />
Bronchial Epithelial <strong>Cell</strong>s 22<br />
Bronchial Smooth Muscle <strong>Cell</strong>s 38<br />
Buffers and Salt Solutions 104<br />
CaCl 2<br />
-Solution 104<br />
Cardiac Fibroblasts 26<br />
Cardiac Microvascular Endothelial <strong>Cell</strong>s 18<br />
Cardiac Myocytes 9<br />
CD14 + Monocytes 53<br />
CD34 + Progenitor <strong>Cell</strong>s 50<br />
CD133 + Progenitor <strong>Cell</strong>s 51<br />
<strong>Cell</strong> Analysis 117<br />
<strong>Cell</strong> Biology Products 116<br />
<strong>Cell</strong> Dissociation Solution ACF 102<br />
<strong>Cell</strong> Model Systems 58<br />
<strong>Cell</strong> Pellets 97<br />
<strong>Cell</strong> Transfection 118<br />
A<br />
B<br />
C<br />
Ham´s F-10 Media 90<br />
Ham´s F-12 Media 90<br />
Leibovitz L-15 Medium 90<br />
McCoy´s 5A Media 91<br />
MEM Media 91<br />
RPMI 1640 Media 91<br />
Collagen Type I Rat Tail 105<br />
Coronary Artery Endothelial <strong>Cell</strong>s 13<br />
Coronary Artery Smooth Muscle <strong>Cell</strong>s 36<br />
Cryo-SFM 85<br />
Cytokine Mix E 87<br />
Cytokines and Growth Factors 106<br />
DC Base Media 89<br />
DC Generation Media 89<br />
Dendritic <strong>Cell</strong> Media 88<br />
Dermal Blood Endothelial <strong>Cell</strong>s 16<br />
Dermal Fibroblasts 25<br />
Dermal Lymphatic Endothelial <strong>Cell</strong>s 17<br />
Dermal Microvascular Endothelial <strong>Cell</strong>s 15<br />
DetachKit 1/2 101<br />
Detachment Reagents 101<br />
Disinfectants 113<br />
DMEM Media 90<br />
DNA Ladders 110<br />
Dulbecco’s PBS 104<br />
Endothelial <strong>Cell</strong> Growth Supplement 105<br />
Endothelial <strong>Cell</strong> Growth Supplement/Heparin 105<br />
Endothelial <strong>Cell</strong> Media 69<br />
Endothelial <strong>Cell</strong> Media MV 70<br />
Endothelial <strong>Cell</strong>s (Large Vessels) 11<br />
Endothelial <strong>Cell</strong>s (Microvascular) 15<br />
Endothelial Progenitor Medium 88<br />
Epidermal Keratinocytes 30<br />
Epidermal Melanocytes 31<br />
Epithelial <strong>Cell</strong> Media 71<br />
Epithelial <strong>Cell</strong>s 21<br />
ESC-Qualified Fetal Calf Serum 93<br />
Extracellular Matrix Proteins 105<br />
D<br />
E<br />
Chondrocyte Media 68
Alphabetical Index 121<br />
Description Page Description Page<br />
Fetal Calf Serum 92<br />
Fibroblast Media 76<br />
Fibroblasts 25<br />
Fibronectin Solutions 105<br />
Fluorescent Labeling 117<br />
Follicle Dermal Papilla <strong>Cell</strong> Media 78<br />
Follicle Dermal Papilla <strong>Cell</strong>s 28<br />
Freezing Medium 85<br />
F<br />
HMEpC 23<br />
hMNC-CB 55<br />
hMNC-PB 55<br />
hMoCD14 + -PB 53<br />
hMo-PB 53<br />
hMSC-AT 48<br />
hMSC-BM 48<br />
hMSC-UC 48<br />
HNEpC 21<br />
HOB 32<br />
HPAEC 14<br />
G-418 Sulphate Solution 103<br />
Gene Cloning, Expression, and Silencing 118<br />
Gentamicin Solution 103<br />
Glutamine, L 105<br />
Glutamine, Stable 105<br />
Ham´s F-10 Media 90<br />
Ham´s F-12 Media 90<br />
Hanks’ BSS 104<br />
HAoAF 26<br />
HAoEC 13<br />
HAoSMC 35<br />
HBdMEC 18<br />
HBdSMC 38<br />
HBEpC 22<br />
HBSMC 38<br />
HBMEC 20<br />
HCAEC 13<br />
HCASMC 36<br />
hCD34 + -CB 50<br />
hCD133 + -CB 51<br />
HCF 26<br />
HCH 10<br />
HCM 9<br />
HCMEC 18<br />
HDBEC 16<br />
HDLEC 17<br />
HDMEC 15<br />
Hematopoietic Progenitor <strong>Cell</strong> Media 87<br />
Hematopoietic Progenitor <strong>Cell</strong> Expansion Medium DXF 87<br />
Hepatocyte Media 79<br />
Hepatocytes 29<br />
HEPES Buffer 104<br />
HEPES Buffered Saline Solution 104<br />
HFDPC 28<br />
HHpC 29<br />
G<br />
H<br />
HPASMC 36<br />
hPC-PL 49<br />
HPF 26<br />
HPlEpC 24<br />
HPMEC 19<br />
HPrSMC 39<br />
HRCEpC 24<br />
HREpC 24<br />
HSAEpC 22<br />
HSaVEC 14<br />
HTEpC 22<br />
HTSMC 37<br />
HUAEC 12<br />
HUASMC 37<br />
HUF 27<br />
Human Insulin 105<br />
Human Primary <strong>Cell</strong>s 8<br />
Cardiac Myocytes 9<br />
Chondrocytes 10<br />
Endothelial <strong>Cell</strong>s (Large Vessels) 11<br />
Endothelial <strong>Cell</strong>s (Microvascular) 15<br />
Epithelial <strong>Cell</strong>s 21<br />
Fibroblasts 25<br />
Follicle Dermal Papilla <strong>Cell</strong>s 28<br />
Hepatocytes 29<br />
Keratinocytes 30<br />
Melanocytes 31<br />
Osteoblasts 32<br />
Preadipocytes 33<br />
Skeletal Muscle <strong>Cell</strong>s 34<br />
Smooth Muscle <strong>Cell</strong>s 35<br />
Human Stem and Blood <strong>Cell</strong>s 46<br />
Human Transferrin 105<br />
HUtMEC 19<br />
HUtSMC 39<br />
HUVEC 12<br />
HVMF 27<br />
HWP 33<br />
Hygromycin B Solution 103
122<br />
Alphabetical Index<br />
Description Page Description Page<br />
I, K, L<br />
Insect <strong>Cell</strong> <strong>Culture</strong> Media 92<br />
Insulin 105<br />
Invasion Kit (HDMEC) 63<br />
Invasion Kit (HUVEC) 63<br />
Keratinocyte Media 80<br />
Keratinocytes 30<br />
Leibovitz L-15 Medium 90<br />
L-Glutamine Solution 105<br />
Lymphatic Endothelial <strong>Cell</strong>s 17<br />
Lymphocyte Separation Medium 1077 89<br />
Mesenchymal Stem <strong>Cell</strong> Chondrogenic Differentiation Medium 86<br />
Mesenchymal Stem <strong>Cell</strong> Growth Medium 86<br />
Mesenchymal Stem <strong>Cell</strong> Neurogenic Differentiation Medium 86<br />
Mesenchymal Stem <strong>Cell</strong> Osteogenic Differentiation Medium 86<br />
Mesenchymal Stem <strong>Cell</strong>s 47<br />
Mesenchymal Stem <strong>Cell</strong>s from Adipose Tissue 48<br />
Mesenchymal Stem <strong>Cell</strong>s from Bone Marrow 48<br />
Mesenchymal Stem <strong>Cell</strong>s from Umbilical Cord Matrix 48<br />
Microbial Detection and Elimination 107<br />
Mineralization Medium Osteoblasts 82<br />
Mitsuhashi/Maramorosh Insect Medium 92<br />
Monocytes 52<br />
Mononuclear <strong>Cell</strong> Medium 89<br />
M<br />
Mammary Epithelial <strong>Cell</strong> Media 73<br />
Mammary Epithelial <strong>Cell</strong>s 23<br />
McCoy´s 5A Media 91<br />
Media and Sera 66<br />
Media for Primary <strong>Cell</strong>s 67<br />
Chondrocyte Media 68<br />
Mononuclear <strong>Cell</strong>s 54<br />
Mononuclear <strong>Cell</strong>s from Cord Blood 55<br />
Mononuclear <strong>Cell</strong>s from Peripheral Blood 55<br />
MSC-Qualified Fetal Calf Serum 93<br />
Mycoplasma Elimination Solutions 110<br />
Mycoplasma Test Kits 109<br />
Mycoplasma-EX Spray 113<br />
Myocyte Media 68<br />
Endothelial <strong>Cell</strong> Media 69<br />
Endothelial Progenitor Medium 88<br />
Epithelial <strong>Cell</strong> Media 71<br />
Fibroblast Media 76<br />
Follicle Dermal Papilla <strong>Cell</strong> Media 78<br />
Hematopoietic Progenitor <strong>Cell</strong> Media 87<br />
Hepatocyte Media 79<br />
Keratinocyte Media 80<br />
Melanocyte Media 81<br />
Mesenchymal Stem <strong>Cell</strong> Differentiation Media 86<br />
Mesenchymal Stem <strong>Cell</strong> Media 86<br />
Mesenchymal Stem <strong>Cell</strong> Growth Medium 86<br />
Mesenchymal Stem <strong>Cell</strong> Growth Medium DXF 86<br />
Monocyte Attachment Medium 89<br />
Mononuclear <strong>Cell</strong> Media 89<br />
Myocyte Media 68<br />
Osteoblast Media 82<br />
Pericyte Media 87<br />
Preadipocyte/Adipocyte Media 83<br />
Skeletal Muscle <strong>Cell</strong> Media 84<br />
Smooth Muscle <strong>Cell</strong> Media 85<br />
Melanocyte Media 81<br />
Melanocytes 31<br />
MEM Alpha Media 91<br />
MEM Amino Acids Solution 105<br />
MEM D-Valine, w/o L-Glutamine 91<br />
MEM Media 91<br />
MEM NEAA Solution 105<br />
MEM Vitamin Solution 105<br />
Mesenchymal Stem <strong>Cell</strong> Adipogenic Differentiation Medium 86<br />
N, O<br />
Nasal Epithelial <strong>Cell</strong>s 21<br />
Neurogenic Differentiation Medium 86<br />
NHDF 25<br />
NHEK 30<br />
NHEM 31<br />
Osteoblasts 32<br />
Osteoblast Media 82<br />
Osteoblast Mineralization Medium 82<br />
Osteogenic Differentiation Medium 86<br />
Overview Human Primary <strong>Cell</strong>s 40<br />
Overview Human Stem and Blood <strong>Cell</strong>s 56<br />
PBS 104<br />
PCR Bacteria Test Kit 112<br />
PCR Mycoplasma Test Kits 109<br />
Pellets 97<br />
Pen/Strep Solution 103<br />
Pen/Strep/Fungizone Solution 103<br />
Pericytes 49<br />
Pericyte Media 87<br />
Pharmacidal Spray 113<br />
Placental Epithelial <strong>Cell</strong> Media 75<br />
Placental Epithelial <strong>Cell</strong>s 24<br />
Planar Migration Assay (HDMEC) 61<br />
Planar Migration Assay (HUVEC) 61<br />
P
Alphabetical Index 123<br />
Description Page Description Page<br />
Preadipocyte Media 83<br />
Preadipocytes 33<br />
Primary <strong>Cell</strong>s 8<br />
PromoKine <strong>Cell</strong> Biology Products 116<br />
Prostate Smooth Muscle <strong>Cell</strong>s 39<br />
Pulmonary Artery Endothelial <strong>Cell</strong>s 14<br />
Pulmonary Artery Smooth Muscle <strong>Cell</strong>s 36<br />
Pulmonary Fibroblasts 26<br />
Pulmonary Microvascular Endothelial <strong>Cell</strong>s 19<br />
U, V, W<br />
Umbilical Artery Endothelial <strong>Cell</strong>s 12<br />
Umbilical Artery Smooth Muscle <strong>Cell</strong>s 37<br />
Umbilical Vein Endothelial <strong>Cell</strong>s 12<br />
Uterine Fibroblasts 27<br />
Uterine Microvascular Endothelial <strong>Cell</strong>s 19<br />
Uterine Smooth Muscle <strong>Cell</strong>s 39<br />
Villous Mesenchymal Fibroblasts 27<br />
White Preadipocytes 33<br />
R<br />
Reagents and Supplements 100<br />
Renal Cortical Epithelial <strong>Cell</strong>s 24<br />
Renal Epithelial <strong>Cell</strong> Media 74<br />
Renal Epithelial <strong>Cell</strong>s 24<br />
RPMI 1640 Media 91<br />
Saphenous Vein Endothelial <strong>Cell</strong>s 14<br />
Schneider’s Drosophila Medium 92<br />
Sera 92<br />
Size Markers 110<br />
Skeletal Muscle <strong>Cell</strong> Media 84<br />
Skeletal Muscle <strong>Cell</strong>s 34<br />
SkMC 34<br />
Small Airway Epithelial <strong>Cell</strong> Media 72<br />
Small Airway Epithelial <strong>Cell</strong>s 22<br />
Smooth Muscle <strong>Cell</strong> Media 85<br />
Smooth Muscle <strong>Cell</strong>s 35<br />
Sodium bicarbonate Solution 104<br />
Stable Glutamine Solution 105<br />
Stem and Blood <strong>Cell</strong>s 46<br />
Supplements 107<br />
S<br />
TC Water 104<br />
Trace Element Mix 105<br />
Tracheal Epithelial <strong>Cell</strong>s 22<br />
Tracheal Smooth Muscle <strong>Cell</strong>s 37<br />
Transferrin 105<br />
Transmigration Kit (HDMEC) 62<br />
Transmigration Kit (HUVEC) 62<br />
Trypsin Neutralizing Solution (TNS) 102<br />
Trypsin Solutions 102<br />
T
124<br />
Numerical Index<br />
Numerical Index<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-12000 – C-12499<br />
C-12255 Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s, pre-screened<br />
12<br />
C-12001 Normal Human Epidermal Keratinocytes,<br />
juvenile foreskin<br />
C-12002 Normal Human Epidermal Keratinocytes,<br />
juvenile foreskin<br />
C-12003 Normal Human Epidermal Keratinocytes,<br />
adult donor<br />
C-12004 Normal Human Epidermal Keratinocytes,<br />
adult donor<br />
C-12005 Normal Human Epidermal Keratinocytes,<br />
juvenile foreskin, pooled<br />
C-12006 Normal Human Epidermal Keratinocytes,<br />
adult donor, pooled<br />
C-12007 Normal Human Epidermal Keratinocytes,<br />
juvenile foreskin, pooled<br />
C-12008 Normal Human Epidermal Keratinocytes,<br />
adult donor, pooled<br />
C-12071 Human Follicle Dermal Papilla <strong>Cell</strong>s 28<br />
C-12072 Human Follicle Dermal Papilla <strong>Cell</strong>s 28<br />
C-12200 Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s, single donor<br />
C-12202 Human Umbilical Artery<br />
Endothelial <strong>Cell</strong>s<br />
C-12203 Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s, pooled<br />
C-12205 Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s, pre-screened<br />
C-12210 Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s, juvenile foreskin<br />
C-12211 Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s, juvenile foreskin<br />
C-12212 Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s, adult donor<br />
C-12214 Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s, juvenile foreskin<br />
C-12215 Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s, pre-screened<br />
C-12216 Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s, juvenile foreskin<br />
C-12217 Human Lymphatic<br />
Endothelial <strong>Cell</strong>s, adult donor<br />
C-12218 Human Dermal Lymphatic<br />
Endothelial <strong>Cell</strong>s, juvenile foreskin<br />
C-12219 Human Lymphatic<br />
Endothelial <strong>Cell</strong>s, adult donor<br />
C-12221 Human Coronary Artery Endothelial <strong>Cell</strong>s 13<br />
C-12222 Human Coronary Artery Endothelial <strong>Cell</strong>s 13<br />
C-12225 Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s, adult donor<br />
C-12226 Human Dermal Blood<br />
Endothelial <strong>Cell</strong>s, adult donor<br />
C-12231 Human Saphenous Vein Endothelial <strong>Cell</strong>s 14<br />
C-12232 Human Saphenous Vein Endothelial <strong>Cell</strong>s 14<br />
C-12241 Human Pulmonary Artery Endothelial <strong>Cell</strong>s 14<br />
C-12242 Human Pulmonary Artery Endothelial <strong>Cell</strong>s 14<br />
C-12250 Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s, single donor<br />
C-12252 Human Umbilical Artery<br />
Endothelial <strong>Cell</strong>s<br />
C-12253 Human Umbilical Vein<br />
Endothelial <strong>Cell</strong>s, pooled<br />
30<br />
30<br />
30<br />
30<br />
30<br />
30<br />
30<br />
30<br />
12<br />
12<br />
12<br />
12<br />
15<br />
16<br />
15<br />
16<br />
15<br />
17<br />
17<br />
17<br />
17<br />
16<br />
16<br />
12<br />
12<br />
12<br />
C-12260 Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s, juvenile foreskin<br />
C-12262 Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s, adult donor<br />
C-12265 Human Dermal Microvascular<br />
Endothelial <strong>Cell</strong>s, pre-screened<br />
C-12271 Human Aortic Endothelial <strong>Cell</strong>s 13<br />
C-12272 Human Aortic Endothelial <strong>Cell</strong>s 13<br />
C-12281 Human Pulmonary Microvascular Endothelial<br />
<strong>Cell</strong>s<br />
C-12282 Human Pulmonary Microvascular Endothelial<br />
<strong>Cell</strong>s<br />
C-12285 Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12286 Human Cardiac Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12287 Human Brain Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12288 Human Brain Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12291 Human Bladder Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12292 Human Bladder Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12295 Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12296 Human Uterine Microvascular<br />
Endothelial <strong>Cell</strong>s<br />
C-12300 Normal Human Dermal Fibroblasts,<br />
juvenile foreskin<br />
C-12302 Human Dermal Fibroblasts,<br />
adult donor<br />
C-12350 Normal Human Dermal Fibroblasts,<br />
juvenile foreskin<br />
C-12352 Human Dermal Fibroblasts, adult donor 25<br />
C-12360 Human Pulmonary Fibroblasts 26<br />
C-12361 Human Pulmonary Fibroblasts 26<br />
C-12375 Human Cardiac Fibroblasts 26<br />
C-12377 Human Cardiac Fibroblasts 26<br />
C-12380 Human Aortic Adventitial Fibroblasts 26<br />
C-12381 Human Aortic Adventitial Fibroblasts 26<br />
C-12385 Human Uterine Fibroblasts 27<br />
C-12386 Human Uterine Fibroblasts 27<br />
C-12390 Human Villous Mesenchymal Fibroblasts 27<br />
C-12391 Human Villous Mesenchymal Fibroblasts 27<br />
C-12395 Human Placental Epithelial <strong>Cell</strong>s 24<br />
C-12397 Human Placental Epithelial <strong>Cell</strong>s 24<br />
C-12400 Normal Human Epidermal Melanocytes,<br />
juvenile foreskin<br />
C-12402 Normal Human Epidermal Melanocytes,<br />
juvenile foreskin, cultured in M2 medium<br />
C-12403 Normal Human Epidermal Melanocytes,<br />
adult donor, cultured in M2 medium<br />
C-12450 Normal Human Epidermal Melanocytes,<br />
juvenile foreskin<br />
C-12452 Normal Human Epidermal Melanocytes,<br />
juvenile foreskin, cultured in M2 medium<br />
C-12453 Normal Human Epidermal Melanocytes,<br />
adult donor, cultured in M2 medium<br />
15<br />
15<br />
15<br />
19<br />
19<br />
18<br />
18<br />
20<br />
20<br />
18<br />
18<br />
19<br />
19<br />
25<br />
25<br />
25<br />
31<br />
31<br />
31<br />
31<br />
31<br />
31
Numerical Index 125<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-12500 – C-19999<br />
C-12901 Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood, single donor, ultra-pure<br />
55<br />
C-12500 Human Umbilical Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
C-12511 Human Coronary Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
C-12512 Human Coronary Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
C-12521 Human Pulmonary Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
C-12522 Human Pulmonary Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
C-12530 Human Skeletal Muscle <strong>Cell</strong>s 34<br />
C-12532 Human Aortic Smooth Muscle <strong>Cell</strong>s 35<br />
C-12533 Human Aortic Smooth Muscle <strong>Cell</strong>s 35<br />
C-12550 Human Umbilical Artery<br />
Smooth Muscle <strong>Cell</strong>s<br />
C-12561 Human Bronchial Smooth Muscle <strong>Cell</strong>s 38<br />
C-12562 Human Bronchial Smooth Muscle <strong>Cell</strong>s 38<br />
C-12565 Human Tracheal Smooth Muscle <strong>Cell</strong>s 37<br />
C-12566 Human Tracheal Smooth Muscle <strong>Cell</strong>s 37<br />
C-12571 Human Bladder Smooth Muscle <strong>Cell</strong>s 38<br />
C-12572 Human Bladder Smooth Muscle <strong>Cell</strong>s 38<br />
C-12573 Human Prostate Smooth Muscle <strong>Cell</strong>s 39<br />
C-12574 Human Prostate Smooth Muscle <strong>Cell</strong>s 39<br />
C-12575 Human Uterine Smooth Muscle <strong>Cell</strong>s 39<br />
C-12576 Human Uterine Smooth Muscle <strong>Cell</strong>s 39<br />
C-12580 Human Skeletal Muscle <strong>Cell</strong>s 34<br />
C-12620 Human Nasal Epithelial <strong>Cell</strong>s 21<br />
C-12621 Human Nasal Epithelial <strong>Cell</strong>s 21<br />
C-12640 Human Bronchial Epithelial <strong>Cell</strong>s 22<br />
C-12641 Human Bronchial Epithelial <strong>Cell</strong>s 22<br />
C-12642 Human Small Airway Epithelial <strong>Cell</strong>s 22<br />
C-12643 Human Small Airway Epithelial <strong>Cell</strong>s 22<br />
C-12644 Human Tracheal Epithelial <strong>Cell</strong>s 22<br />
C-12645 Human Tracheal Epithelial <strong>Cell</strong>s 22<br />
C-12650 Human Mammary Epithelial <strong>Cell</strong>s 23<br />
C-12651 Human Mammary Epithelial <strong>Cell</strong>s 23<br />
C-12660 Human Renal Cortical Epithelial <strong>Cell</strong>s 24<br />
C-12662 Human Renal Cortical Epithelial <strong>Cell</strong>s 24<br />
C-12665 Human Renal Epithelial <strong>Cell</strong>s 24<br />
C-12666 Human Renal Epithelial <strong>Cell</strong>s 24<br />
C-12710 Human Chondrocytes 10<br />
C-12720 Human Osteoblasts 32<br />
C-12730 Human White Preadipocytes, subcutaneus 33<br />
C-12731 Human White Preadipocytes, subcutaneus 33<br />
C-12732 Human White Preadipocytes, visceral 33<br />
C-12733 Human White Preadipocytes, visceral 33<br />
C-12750 Human Chondrocytes 10<br />
C-12760 Human Osteoblasts 32<br />
C-12810 Human Cardiac Myocytes 9<br />
C-12811 Human Cardiac Myocytes 9<br />
C-12850 Human Hepatocytes 29<br />
37<br />
36<br />
36<br />
36<br />
36<br />
37<br />
C-12904 Human Mononuclear <strong>Cell</strong>s<br />
from Cord Blood, pooled, ultra-pure<br />
C-12907 Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood, single donor,<br />
ultra-pure<br />
C-12908 Human Mononuclear <strong>Cell</strong>s<br />
from Peripheral Blood, pooled, ultra-pure<br />
C-12909 Human CD14 + Monocytes from<br />
Peripheral Blood, single donor<br />
C-12910 Human Monocytes from<br />
Peripheral Blood, single donor<br />
C-12912 Human CD14 + Monocytes from<br />
Peripheral Blood, single donor, pre-screened<br />
C-12913 Human Monocytes from<br />
Peripheral Blood, single donor, pre-screened<br />
C-12921 Human CD34 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood, single donor<br />
C-12924 Human CD34 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood, pooled<br />
C-12927 Human CD133 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood single donor<br />
C-12929 Human CD133 + Progenitor <strong>Cell</strong>s<br />
from Cord Blood pooled<br />
C-12971 Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Umbilical Cord Matrix<br />
C-12972 Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Umbilical Cord Matrix<br />
C-12974 Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Bone Marrow<br />
C-12975 Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Bone Marrow<br />
C-12977 Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Adipose Tissue<br />
C-12978 Human Mesenchymal Stem <strong>Cell</strong>s<br />
from Adipose Tissue<br />
C-12980 Human Pericytes from Placenta 49<br />
C-12981 Human Pericytes from Placenta 49<br />
C-13010 3D-Angiogenesis Assay (HUVEC) 60<br />
C-13011 3D-Angiogenesis Assay (HUVEC) 60<br />
C-13012 3D-Angiogenesis Assay (HUVEC) 60<br />
C-13020 3D-Angiogenesis Assay (HDMEC) 60<br />
C-13021 3D-Angiogenesis Assay (HDMEC) 60<br />
C-13022 3D-Angiogenesis Assay (HDMEC) 60<br />
C-13030 Planar Migration Assay (HUVEC) 61<br />
C-13031 Planar Migration Assay (HDMEC) 61<br />
C-13035 Transmigration Kit (HUVEC) 62<br />
C-13036 Transmigration Kit (HDMEC) 62<br />
C-13040 Invasion Kit (HUVEC) 63<br />
C-13041 Invasion Kit (HDMEC) 63<br />
C-14001 NHEK.f Pellet 98<br />
C-14002 NHEK adult Pellet 98<br />
C-14003 NHEK. f pooled Pellet 98<br />
C-14004 NHEK adult pooled Pellet 98<br />
C-14005 HFDPC Pellet 97<br />
C-14010 HUVEC Pellet 98<br />
C-14011 HUVEC pooled Pellet 98<br />
55<br />
55<br />
55<br />
53<br />
53<br />
53<br />
53<br />
50<br />
50<br />
51<br />
51<br />
48<br />
48<br />
48<br />
48<br />
48<br />
48
126<br />
Numerical Index<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-14012 HUVEC pre-screened Pellet 98<br />
C-14013 HUAEC Pellet 98<br />
C-14015 HDMEC Pellet 97<br />
C-14016 HDMEC adult Pellet 97<br />
C-14017 HDMEC pre-screened Pellet 97<br />
C-14018 HDBEC Pellet 97<br />
C-14019 HDBEC adult Pellet 97<br />
C-14020 HDLEC Pellet 97<br />
C-14021 HDLEC adult Pellet 97<br />
C-14022 HCAEC Pellet 97<br />
C-14023 HAoEC Pellet 97<br />
C-14024 HPAEC Pellet 98<br />
C-14025 HSaVEC Pellet 98<br />
C-14026 HBdMEC Pellet 97<br />
C-14027 HPMEC Pellet 98<br />
C-14028 HUtMEC Pellet 98<br />
C-14029 HCMEC Pellet 97<br />
C-14030 NHDF Pellet 98<br />
C-14031 NHDF adult Pellet 98<br />
C-14032 HBMEC Pellet 97<br />
C-14034 HVMF Pellet 98<br />
C-14035 HPF Pellet 98<br />
C-14036 HCF Pellet 97<br />
C-14037 HAoAF Pellet 97<br />
C-14038 HUF Pellet 98<br />
C-14040 NHEM.f Pellet 98<br />
C-14042 NHEM.f M2 Pellet 98<br />
C-14043 NHEM M2 adult Pellet 98<br />
C-14050 HUASMC Pellet 98<br />
C-14051 HPASMC Pellet 98<br />
C-14052 HCASMC Pellet 97<br />
C-14053 HAoSMC Pellet 97<br />
C-14055 HBSMC Pellet 97<br />
C-14056 HTSMC Pellet 98<br />
C-14057 HBdSMC Pellet 97<br />
C-14058 HPrSMC Pellet 98<br />
C-14059 HUtSMC Pellet 98<br />
C-14060 SkMC Pellet 98<br />
C-14062 HNEpC Pellet 98<br />
C-14063 HBEpC Pellet 97<br />
C-14064 HTEpC Pellet 98<br />
C-14065 HSAEpC Pellet 98<br />
C-14066 HMEpC Pellet 97<br />
C-14067 HREpC Pellet 98<br />
C-14068 HRCEpC Pellet 98<br />
C-14069 HPlEpC Pellet 98<br />
C-14070 HCH Pellet 97<br />
C-14071 HOB Pellet 98<br />
C-14072 HWP subcutaneous Pellet 98<br />
C-14073 HWP visceral Pellet 98<br />
C-14080 HCM Pellet 97<br />
C-14085 HHpC Pellet 97<br />
C-14090 hMSC-BM Pellet 98<br />
C-14091 hMSC-UC Pellet 98<br />
C-14092 hMSC-AT Pellet 98<br />
C-14095 hPC-PL Pellet 98<br />
C-14096 hMNC-CB single donor Pellet 97<br />
C-14097 hMNC-CB pooled Pellet 97<br />
C-14098 hMNC-PB single donor Pellet 97<br />
C-14099 hMNC-PB pooled Pellet 97<br />
C-14110 hMo-CD14 + -PB single donor Pellet 98<br />
C-14111 hMo-PB single donor Pellet 98<br />
C-14112 hMo-CD14 + -PB single donor<br />
pre-screened Pellet<br />
C-14113 hMo-PB single donor pre-screened Pellet 98<br />
C-20000 – C-24999<br />
C-20011 Keratinocyte Growth Medium 2 80<br />
C-20111 Keratinocyte Growth Medium 2 Kit 80<br />
C-20211 Keratinocyte Basal Medium 2 80<br />
C-20216 Keratinocyte Basal Medium 2,<br />
phenol red-free<br />
C-21010 Mammary Epithelial <strong>Cell</strong> Growth Medium 73<br />
C-21060 Airway Epithelial <strong>Cell</strong> Growth Medium 71<br />
C-21070 Small Airway Epithelial <strong>Cell</strong><br />
Growth Medium<br />
C-21110 Mammary Epithelial <strong>Cell</strong><br />
Growth Medium Kit<br />
C-21160 Airway Epithelial <strong>Cell</strong> Growth Medium Kit 71<br />
C-21170 Small Airway Epithelial <strong>Cell</strong><br />
Growth Medium Kit<br />
C-21210 Mammary Epithelial <strong>Cell</strong> Basal Medium 73<br />
C-21215 Mammary Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-21260 Airway Epithelial <strong>Cell</strong> Basal Medium 71<br />
C-21265 Airway Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-21270 Small Airway Epithelial <strong>Cell</strong> Basal Medium 72<br />
C-21275 Small Airway Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-22010 Endothelial <strong>Cell</strong> Growth Medium 69<br />
C-22011 Endothelial <strong>Cell</strong> Growth Medium 2 69<br />
C-22020 Endothelial <strong>Cell</strong> Growth Medium MV 70<br />
C-22022 Endothelial <strong>Cell</strong> Growth Medium MV 2 70<br />
C-22062 Smooth Muscle <strong>Cell</strong> Growth Medium 2 85<br />
C-22070 Myocyte Growth Medium 68<br />
C-22110 Endothelial <strong>Cell</strong> Growth Medium Kit 69<br />
C-22111 Endothelial <strong>Cell</strong> Growth Medium 2 Kit 69<br />
C-22120 Endothelial <strong>Cell</strong> Growth Medium MV Kit 70<br />
C-22121 Endothelial <strong>Cell</strong> Growth Medium MV 2 Kit 70<br />
C-22162 Smooth Muscle <strong>Cell</strong> Growth Medium 2 Kit 85<br />
C-22170 Myocyte Growth Medium Kit 68<br />
C-22210 Endothelial <strong>Cell</strong> Basal Medium 69<br />
98<br />
80<br />
72<br />
73<br />
72<br />
73<br />
71<br />
72
Numerical Index 127<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-22211 Endothelial <strong>Cell</strong> Basal Medium 2 69<br />
C-22215 Endothelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-22216 Endothelial <strong>Cell</strong> Basal Medium 2,<br />
phenol red-free<br />
C-22220 Endothelial <strong>Cell</strong> Basal Medium MV 70<br />
C-22221 Endothelial <strong>Cell</strong> Basal Medium MV 2 70<br />
C-22225 Endothelial <strong>Cell</strong> Basal Medium MV,<br />
phenol red-free<br />
C-22226 Endothelial <strong>Cell</strong> Basal Medium MV 2,<br />
phenol red-free<br />
C-22262 Smooth Muscle <strong>Cell</strong> Basal Medium 2 85<br />
C-22267 Smooth Muscle <strong>Cell</strong> Basal Medium 2,<br />
phenol red-free<br />
C-22270 Myocyte Basal Medium 68<br />
C-22275 Myocyte Basal Medium,<br />
phenol red-free<br />
C-23010 Fibroblast Growth Medium 76<br />
C-23020 Fibroblast Growth Medium 2 77<br />
C-23025 Fibroblast Growth Medium 3 77<br />
C-23060 Skeletal Muscle <strong>Cell</strong> Growth Medium 84<br />
C-23061 Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium<br />
C-23110 Fibroblast Growth Medium Kit 76<br />
C-23120 Fibroblast Growth Medium 2 Kit 77<br />
C-23130 Fibroblast Growth Medium 3 Kit 77<br />
C-23160 Skeletal Muscle <strong>Cell</strong> Growth Medium Kit 84<br />
C-23210 Fibroblast Basal Medium 76<br />
C-23215 Fibroblast Basal Medium, phenol red-free 76<br />
C-23220 Fibroblast Basal Medium 2 77<br />
C-23225 Fibroblast Basal Medium 2, phenol red-free 77<br />
C-23230 Fibroblast Basal Medium 3 77<br />
C-23235 Fibroblast Basal Medium 3, phenol red-free 77<br />
C-23260 Skeletal Muscle <strong>Cell</strong> Basal Medium 84<br />
C-23265 Skeletal Muscle <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-24010 Melanocyte Growth Medium 81<br />
C-24110 Melanocyte Growth Medium Kit 81<br />
C-24210 Melanocyte Basal Medium 81<br />
C-24215 Melanocyte Basal Medium, phenol red-free 81<br />
C-24300 Melanocyte Growth Medium M2 81<br />
C-24305 Melanocyte Growth Medium M2,<br />
phenol red-free<br />
C-24400 Melanocyte M2 Basal Medium 81<br />
C-24405 Melanocyte M2 Basal Medium,<br />
phenol red-free<br />
C-25000 – C-29999<br />
C-25010 Hepatocyte Growth Medium 79<br />
C-25020 Hepatocyte Maintenance Medium 79<br />
C-25110 Hepatocyte Growth Medium Kit 79<br />
C-25120 Hepatocyte Maintenance Medium Kit 79<br />
C-25210 Hepatocyte Basal Medium 79<br />
C-25215 Hepatocyte Basal Medium, phenol red-free 79<br />
69<br />
69<br />
70<br />
70<br />
85<br />
68<br />
84<br />
84<br />
81<br />
81<br />
C-26001 Renal Epithelial <strong>Cell</strong> Growth Medium 74<br />
C-26011 Renal Epithelial <strong>Cell</strong> Growth Medium Kit 74<br />
C-26021 Renal Epithelial <strong>Cell</strong> Basal Medium 74<br />
C-26022 Renal Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-26030 Renal Epithelial <strong>Cell</strong> Growth Medium 2 74<br />
C-26040 Placental Epithelial <strong>Cell</strong> Growth Medium 75<br />
C-26130 Renal Epithelial <strong>Cell</strong> Growth Medium 2 Kit 74<br />
C-26140 Placental Epithelial <strong>Cell</strong> Growth Medium Kit 75<br />
C-26230 Renal Epithelial <strong>Cell</strong> Basal Medium 2 74<br />
C-26235 Renal Epithelial <strong>Cell</strong> Basal Medium 2,<br />
phenol red-free<br />
C-26240 Placental Epithelial <strong>Cell</strong> Basal Medium 75<br />
C-26245 Placental Epithelial <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-26500 Follicle Dermal Papilla <strong>Cell</strong> Basal Medium 78<br />
C-26501 Follicle Dermal Papilla <strong>Cell</strong> Growth Medium 78<br />
C-26502 Follicle Dermal Papilla <strong>Cell</strong><br />
Growth Medium Kit<br />
C-26505 Follicle Dermal Papilla <strong>Cell</strong> Basal Medium,<br />
phenol red-free<br />
C-27001 Osteoblast Growth Medium 82<br />
C-27010 Osteoblast Basal Medium 82<br />
C-27015 Osteoblast Basal Medium, phenol red-free 82<br />
C-27020 Osteoblast Mineralization Medium 82<br />
C-27101 Chondrocyte Growth Medium 68<br />
C-27111 Chondrocyte Basal Medium 68<br />
C-27115 Chondrocyte Basal Medium,<br />
phenol red-free<br />
C-27410 Preadipocyte Growth Medium 83<br />
C-27411 Preadipocyte Basal Medium 83<br />
C-27415 Preadipocyte Basal Medium,<br />
phenol red-free<br />
C-27417 Preadipocyte Growth Medium Kit 83<br />
C-27431 Adipocyte Basal Medium 83<br />
C-27435 Adipocyte Basal Medium, phenol red-free 83<br />
C-27436 Preadipocyte Differentiation Medium 83<br />
C-27437 Preadipocyte Differentiation Medium Kit 83<br />
C-27438 Adipocyte Nutrition Medium 83<br />
C-27439 Adipocyte Nutrition Medium Kit 83<br />
C-28010 Mesenchymal Stem <strong>Cell</strong> Growth Medium 86<br />
C-28011 Mesenchymal Stem <strong>Cell</strong> Adipogenic<br />
Differentiation Medium<br />
C-28012 Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium<br />
C-28013 Mesenchymal Stem <strong>Cell</strong> Osteogenic<br />
Differentiation Medium<br />
C-28014 Mesenchymal Stem <strong>Cell</strong> Chondrogenic<br />
Differentiation Medium w/o Inducers<br />
C-28015 Mesenchymal Stem <strong>Cell</strong> Neurogenic<br />
Differentiation Medium<br />
C-28019 Mesenchymal Stem <strong>Cell</strong><br />
Growth Medium DXF<br />
C-28020 Hematopoietic Progenitor <strong>Cell</strong> Medium 87<br />
C-28021 Hematopoietic Progenitor <strong>Cell</strong> Expansion<br />
Medium DXF<br />
C-28025 Endothelial Progenitor Medium 88<br />
74<br />
74<br />
75<br />
78<br />
78<br />
68<br />
83<br />
86<br />
86<br />
86<br />
86<br />
86<br />
86<br />
87
128<br />
Numerical Index<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-28030 Mononuclear <strong>Cell</strong> Medium 89<br />
C-28040 Pericyte Growth Medium 87<br />
C-28050 DC Generation Medium 89<br />
C-28051 Monocyte Attachment Medium 89<br />
C-28052 DC Generation Medium DXF 89<br />
C-28053 DC Base Medium 89<br />
C-28054 DC Base Medium DXF 89<br />
C-29910 Cryo-SFM 85<br />
C-29912 Cryo-SFM 85<br />
C-39220 Endothelial <strong>Cell</strong> Growth Medium MV<br />
SupplementPack<br />
C-39221 Endothelial <strong>Cell</strong> Growth Medium MV 2<br />
SupplementPack<br />
C-39225 Endothelial <strong>Cell</strong> Growth Medium MV<br />
Supplement Mix<br />
C-39226 Endothelial <strong>Cell</strong> Growth Medium MV 2<br />
Supplement Mix<br />
C-39262 Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />
SupplementPack<br />
C-39267 Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />
Supplement Mix<br />
70<br />
70<br />
70<br />
70<br />
85<br />
85<br />
C-30000 – C-39999<br />
C-30020 Bovine Pituitary Extract 15 105<br />
C-30021 Bovine Pituitary Extract 26 105<br />
C-30040 Bovine Pituitary Extract 15 105<br />
C-30041 Bovine Pituitary Extract 26 105<br />
C-30120 Endothelial <strong>Cell</strong> Growth Supplement/<br />
Heparin<br />
C-30140 Endothelial <strong>Cell</strong> Growth Supplement/<br />
Heparin<br />
105<br />
105<br />
C-30160 Endothelial <strong>Cell</strong> Growth Supplement 105<br />
C-30180 Endothelial <strong>Cell</strong> Growth Supplement 105<br />
C-34006 CaCl 2<br />
-Solution (0.5 M) 104<br />
C-37350 Fetal Calf Serum (EU Quality)<br />
human cell culture tested<br />
C-37355 Fetal Calf Serum (EU Quality)<br />
human cell culture tested<br />
C-37385 Fetal Calf Serum (EU Quality)<br />
Mesenchymal Stem <strong>Cell</strong> Qualified<br />
C-37386 Fetal Calf Serum (EU Quality)<br />
Mesenchymal Stem <strong>Cell</strong> Qualified<br />
C-37387 Fetal Calf Serum (EU Quality)<br />
Embryonic Stem <strong>Cell</strong> qualified<br />
C-37388 Fetal Calf Serum (EU Quality)<br />
Embryonic Stem <strong>Cell</strong> qualified<br />
C-39011 Keratinocyte Growth Medium 2<br />
SupplementPack<br />
C-39016 Keratinocyte Growth Medium 2<br />
Supplement Mix<br />
C-39110 Mammary Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39115 Mammary Epithelial <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39160 Airway Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39165 Airway Epithelial <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39170 Small Airway Epithelial <strong>Cell</strong> Growth<br />
Medium SupplementPack<br />
C-39175 Small Airway Epithelial <strong>Cell</strong> Growth<br />
Medium Supplement Mix<br />
C-39210 Endothelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39211 Endothelial <strong>Cell</strong> Growth Medium 2<br />
SupplementPack<br />
C-39215 Endothelial <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39216 Endothelial <strong>Cell</strong> Growth Medium 2<br />
Supplement Mix<br />
92<br />
92<br />
93<br />
93<br />
93<br />
93<br />
80<br />
80<br />
73<br />
73<br />
71<br />
71<br />
72<br />
72<br />
69<br />
69<br />
69<br />
69<br />
C-39270 Myocyte Growth Medium<br />
SupplementPack<br />
C-39275 Myocyte Growth Medium Supplement Mix 68<br />
C-39310 Fibroblast Growth Medium<br />
SupplementPack<br />
C-39315 Fibroblast Growth Medium<br />
Supplement Mix<br />
C-39320 Fibroblast Growth Medium 2<br />
SupplementPack<br />
C-39325 Fibroblast Growth Medium 2<br />
Supplement Mix<br />
C-39345 Fibroblast Growth Medium 3<br />
Supplement Mix<br />
C-39350 Fibroblast Growth Medium 3<br />
SupplementPack<br />
C-39360 Skeletal Muscle <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39365 Skeletal Muscle <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39366 Skeletal Muscle <strong>Cell</strong> Differentiation<br />
Medium Supplement Mix<br />
C-39410 Melanocyte Growth Medium<br />
SupplementPack<br />
C-39415 Melanocyte Growth Medium<br />
Supplement Mix<br />
C-39420 Melanocyte Growth Medium M2<br />
Supplement Mix<br />
C-39425 Preadipocyte Growth Medium<br />
Supplement Mix<br />
C-39427 Preadipocyte Growth Medium<br />
SupplementPack<br />
C-39436 Preadipocyte Differentiation Medium<br />
Supplement Mix<br />
C-39437 Preadipocyte Differentiation Medium<br />
SupplementPack<br />
C-39438 Adipocyte Nutrition Medium<br />
Supplement Mix<br />
C-39439 Adipocyte Nutrition Medium<br />
SupplementPack<br />
C-39600 Renal Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39604 Renal Epithelial <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39605 Renal Epithelial <strong>Cell</strong> Growth Medium 2<br />
SupplementPack<br />
C-39606 Renal Epithelial <strong>Cell</strong> Growth Medium 2<br />
Supplement Mix<br />
C-39610 Placental Epithelial <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39612 Placental Epithelial <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39615 Osteoblast Growth Medium<br />
Supplement Mix<br />
68<br />
76<br />
76<br />
77<br />
77<br />
77<br />
77<br />
84<br />
84<br />
84<br />
81<br />
81<br />
81<br />
83<br />
83<br />
83<br />
83<br />
83<br />
83<br />
74<br />
74<br />
74<br />
74<br />
75<br />
75<br />
82
Numerical Index 129<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-39620 Follicle Dermal Papilla <strong>Cell</strong> Growth Medium<br />
SupplementPack<br />
C-39625 Follicle Dermal Papilla <strong>Cell</strong> Growth Medium<br />
Supplement Mix<br />
C-39635 Chondrocyte Growth Medium<br />
Supplement Mix<br />
C-39640 Hepatocyte Growth Medium<br />
SupplementPack<br />
C-39642 Hepatocyte Growth Medium<br />
Supplement Mix<br />
C-39650 Hepatocyte Maintenance Medium<br />
SupplementPack<br />
C-39652 Hepatocyte Maintenance Medium<br />
Supplement Mix<br />
C-39890 Cytokine Mix E for HPC-Expansion<br />
Medium DXF<br />
C-39891 Cytokine Mix E for HPC-Expansion<br />
Medium DXF<br />
C-40000 – C-49999<br />
78<br />
78<br />
68<br />
79<br />
79<br />
79<br />
79<br />
87<br />
87<br />
C-41222 DetachKit 2-250 101<br />
C-41310 Accutase-Solution,<br />
primary human cell culture tested<br />
101<br />
C-41320 <strong>Cell</strong> Dissociation Solution ACF 102<br />
C-42010 Pen/Strep Solution 103<br />
C-42020 Pen/Strep/Fungizone Solution 103<br />
C-42040 Amphotericin B Solution 103<br />
C-42060 Gentamicin Solution 103<br />
C-42094 G-418 Sulphate Solution 103<br />
C-42095 Hygromycin B Solution 103<br />
C-42210 L-Glutamine Solution 105<br />
C-42215 Stable Glutamine Solution 105<br />
C-42320 MEM Amino Acids Solution 105<br />
C-42322 MEM NEAA Solution 105<br />
C-42328 MEM Vitamin Solution 105<br />
C-42331 Trace Element Mix (1000x) 105<br />
C-42340 Sodium Bi-Carbonate Solution 104<br />
C-43010 Collagen Type I Rat Tail 105<br />
C-40000 HEPES Buffered Saline Solution 104<br />
C-40010 HEPES Buffered Saline Solution 104<br />
C-40020 HEPES Buffered Saline Solution 104<br />
C-40030 HEPES Buffer (1 M) 104<br />
C-40230 Dulbecco's PBS 104<br />
C-43020 Collagen Type I Rat Tail 105<br />
C-43050 Fibronectin Solution Bovine 105<br />
C-43060 Fibronectin Solution Human 105<br />
C-44010 Lymphocyte Separation Medium 1077 89<br />
C-49998 TC Water 104<br />
C-40232 Dulbecco's PBS, w/o Ca ++ / Mg ++ 104<br />
C-40238 Dulbecco's PBS, w/o Ca ++ /Mg ++ , 10x conc. 104<br />
C-40240 PBS, endotoxin free 104<br />
C-40242 PBS, w/o Ca ++ /Mg ++ , endotoxin free 104<br />
C-40310 Hanks' BSS 104<br />
C-40320 Hanks' BSS, w/o Ca ++ /Mg ++ 104<br />
C-40370 Hanks' BSS, phenol red-free 104<br />
C-40390 Hanks' BSS, w/o Ca ++ /Mg ++ , phenol<br />
red-free<br />
104<br />
C-41000 Trypsin / EDTA 102<br />
C-41002 Trypsin / EDTA 2 102<br />
C-41010 Trypsin / EDTA 102<br />
C-41012 Trypsin / EDTA 2 102<br />
C-41020 Trypsin / EDTA 102<br />
C-41022 Trypsin / EDTA 2 102<br />
C-41040 Trypsin PBS 1:250, w/o Ca ++ /Mg ++ 102<br />
C-41050 Trypsin PBS 1:250, w/o Ca ++ /Mg ++ , 10x<br />
conc., CC grade<br />
102<br />
C-41060 Trypsin-EDTA PBS 1:250 102<br />
C-41070 Trypsin-EDTA PBS 1:250, w/o Ca ++ /Mg ++ ,<br />
10x conc., CC grade<br />
102<br />
C-41100 Trypsin Neutralizing Solution 102<br />
C-41110 Trypsin Neutralizing Solution 102<br />
C-41120 Trypsin Neutralizing Solution 102<br />
C-41200 DetachKit-30 101<br />
C-41202 DetachKit 2-30 101<br />
C-50000 – C-69999<br />
C-52310 Human Insulin, human cell culture tested 105<br />
C-52322 Human Transferrin APO, low iron content 105<br />
C-52332 Human Transferrin HOLO, iron saturated 105<br />
C-60170 EGF, human, recombinant (E. coli) 106<br />
C-60182 EGF, human, recombinant (Pichia) 106<br />
C-60212 Insulin, human, recombinant (E. coli) 106<br />
C-60240 FGF-2 (FGF-b), human, recombinant (E. coli) 106<br />
C-60340 FGF-1 (FGF-a), human, recombinant (E. coli) 106<br />
C-60420 GM-CSF, human, recombinant (E. coli) 106<br />
C-60434 G-CSF, human, recombinant (E. coli) 106<br />
C-60442 M-CSF, human, recombinant (E. coli) 106<br />
C-61301 IL-3 CC, human, recombinant (E. coli) 106<br />
C-61401 IL-4 CC, human, recombinant (E. coli) 106<br />
C-61601 IL-6 CC, human, recombinant (E. coli) 106<br />
C-63120 SCF, human, recombinant (E. coli) 106<br />
C-63200 sRANKL, human recombinant (E. coli) 106<br />
C-63722 TNF-alpha, human, recombinant (E. coli) 106<br />
C-64420 VEGF-165, human, recombinant (E. coli) 106<br />
C-64423 VEGF-165, human, recombinant (HEK293) 106<br />
C-65112 TPO, human, recombinant (E. coli) 106<br />
C-67110 flt-3 Ligand, human, recombinant (E. coli) 106<br />
C-41210 DetachKit-125 101<br />
C-41212 DetachKit 2-125 101<br />
C-41220 DetachKit-250 101
130<br />
Numerical Index<br />
Cat. Number Product Page Cat. Number Product Page<br />
C-70000 – C-74999<br />
C-71110 DMEM, w/o L-Glutamine 90<br />
C-71111 DMEM, with L-Glutamine 90<br />
C-71115 DMEM, with 25 mM HEPES,<br />
w/o L-Glutamine<br />
C-71116 DMEM, with 25 mM HEPES,<br />
with L-Glutamine<br />
C-71120 DMEM, with stable Glutamine 90<br />
C-71210 DMEM, High Glucose (4.5g/l),<br />
w/o L-Glutamine<br />
C-71212 DMEM, High Glucose (4.5g/l),<br />
with L-Glutamine<br />
C-71220 DMEM, High Glucose (4.5g/l),<br />
with stable Glutamine<br />
C-71221 DMEM, High Glucose (4.5g/l),<br />
with stable Glutamine, with Na-Pyruvate<br />
C-71222 DMEM, High Glucose (4.5g/l),<br />
with L-Glutamine, with Na-Pyruvate<br />
C-72223 DMEM, High Glucose (4.5g/l),<br />
w/o L-Glutamine, with Na-Pyruvate<br />
C-71310 DMEM/Ham´s F-12, w/o L-Glutamine 90<br />
C-71311 DMEM/Ham´s F-12, with L-Glutamine 90<br />
C-71320 DMEM/Ham´s F-12, with stable Glutamine 90<br />
C-72010 Ham´s F-10, w/o L-Glutamine 90<br />
C-72020 Ham´s F-10, with L-Glutamine 90<br />
C-72110 Ham´s F-12, w/o L-Glutamine 90<br />
C-72120 Ham´s F-12, with L-Glutamine 90<br />
C-73110 Leibovitz L-15, w/o L-Glutamine 90<br />
C-73210 McCoy's 5A, w/o L-Glutamine 91<br />
C-73211 McCoy’s 5A, with L-Glutamine 91<br />
C-73220 McCoy´s 5A, with stable Glutamine 91<br />
C-73227 McCoy´s 5A, with stable Glutamine,<br />
phenol red-free<br />
C-75000 – C-79999<br />
90<br />
90<br />
90<br />
90<br />
90<br />
90<br />
90<br />
90<br />
91<br />
PK<br />
PK-CA91-1024 PCR Mycoplasma Test Kit I/C 110<br />
PK-CA91-1048 PCR Mycoplasma Test Kit I/C 110<br />
PK-CA91-1096 PCR Mycoplasma Test Kit I/C 110<br />
PK-CA91-2024 PCR Bacteria Test Kit 112<br />
PK-CA91-2048 PCR Bacteria Test Kit 112<br />
PK-CA91-3025A PCR Mycoplasma Test Kit I/RT; Variant A 110<br />
PK-CA91-3025B PCR Mycoplasma Test Kit I/RT; Variant B 110<br />
PK-CA92-100 PCR Mycoplasma Test Kit III 110<br />
PK-CC01-867-1B Aquaguard-1 (incubator) 113<br />
PK-CC01-867-10B Aquaguard-1 (incubator) 113<br />
PK-CC01-916-1E Aquaguard-2 (water bath) 113<br />
PK-CC01-916-10E Aquaguard-2 (water bath) 113<br />
PK-CC03-036-1B BIOMYC-1 111<br />
PK-CC03-036-1C BIOMYC-1 111<br />
PK-CC03-036-1D BIOMYC-1 111<br />
PK-CC03-037-1B BIOMYC-2 111<br />
PK-CC03-037-1C BIOMYC-2 111<br />
PK-CC03-037-1D BIOMYC-2 111<br />
PK-CC03-038-1B BIOMYC-3 111<br />
PK-CC03-038-1C BIOMYC-3 111<br />
PK-CC03-038-1D BIOMYC-3 111<br />
PK-CC-110100 Pharmacidal Spray 113<br />
PK-CC-110100-5L Pharmacidal Spray 113<br />
PK-CC91-4003 Mycoplasma-EX 111<br />
PK-CC91-5050 Mycoplasma-EX Spray 113<br />
PK-CC91-5050-5L Mycoplasma-EX Spray 113<br />
PK-CC91-5060 Mycoplasma Disinfection Kit I 113<br />
PK-CC91-5070 Mycoplasma Disinfection Kit II 113<br />
PK-MB713-001N RTU DNA Ladder I (100 bp) 110<br />
PK-MB713-002N RTU DNA Ladder II (1000 bp) 110<br />
C-75012 MEM Alpha, w/o Nucleosides,<br />
w/o L-Glutamine<br />
C-75013 MEM Alpha, w/o Nucleosides,<br />
with L-Glutamine<br />
C-75014 MEM Alpha, with Nucleosides,<br />
with L-Glutamine<br />
91<br />
91<br />
91<br />
C-75100 MEM D-Valine, w/o L-Glutamine 91<br />
C-75210 MEM Earl, w/o L-Glutamine 91<br />
C-75211 MEM Earl, with L-Glutamine 91<br />
C-75220 MEM Earl, with stable Glutamine 91<br />
C-76010 RPMI 1640, w/o L-Glutamine 91<br />
C-76011 RPMI 1640, with L-Glutamine 91<br />
C-76015 RPMI 1640, with 25mM HEPES,<br />
w/o L-Glutamine<br />
C-76016 RPMI 1640, with 25mM HEPES,<br />
with L-Glutamine<br />
C-76017 RPMI 1640, w/o L-Glutamine,<br />
phenol red-free<br />
91<br />
91<br />
91<br />
C-76020 RPMI 1640, with stable Glutamine 91<br />
C-77110 Mitsuhashi/Maramorosh Insect Medium 92<br />
C-77210 Schneider's Drosophila Medium,<br />
w/o L-Glutamine<br />
92
International Distributors 131<br />
International Distributors<br />
Cat. AlbaniaNumber Product Biomedica Page Shpk<br />
Cat. Number Product Phone: +355-69 40 91 613 Page<br />
Rr Themistokli Germenji<br />
Fax: +355-45 00 681<br />
Kulla Ambasador, Kati 3, Ap 1<br />
Email: a.selimi@bmgrp.al<br />
1000 Tirana<br />
Internet: www.biomedica.co.at<br />
Albania<br />
Australia<br />
Banksia Scientific Company<br />
3/160 Lytton Road<br />
PO Box 529<br />
Bulimba QLD 4171<br />
Australia<br />
Phone: +61-7-39 02 30 00<br />
Fax: +61-7-32 17 98 69<br />
Free Call: 1300 769944<br />
Email: sales@banksiascientific.com.au<br />
Internet: www.banksiascientific.com.au<br />
Life Research Pty Ltd.<br />
Level 1, 5 Lakeside Drive<br />
Burwood East Victoria 3151<br />
Australia<br />
Phone: +61-3-98 03 00 45<br />
Fax: +61-3-86 48 58 41<br />
Email: info@liferesearch.com<br />
Internet: www.liferesearch.com<br />
Austria<br />
Biomedica Medizinprodukte GmbH & Co KG<br />
Divischgasse 4<br />
1210 Wien<br />
Austria<br />
Phone: +43-1-29 10 75 6<br />
Fax: +43-1-29 01 42 9<br />
Email: sales.biomedica@bmgrp.at<br />
Internet: www.biomedica.co.at<br />
Bangladesh<br />
KRISHGEN BioSystems<br />
Unit Nos#318/319<br />
3rd Floor<br />
Shah & Nahar Ind. Premises<br />
Dr E Moses Road<br />
Worli<br />
Mumbai 400018<br />
India<br />
Phone: +91-22-66 37 29 90<br />
Fax: +91-22-66 37 29 91<br />
Email: sales@krishgen.com<br />
Internet: www.krishgen.com<br />
Bosnia-Herzegovina<br />
Biomedica d.o.o.<br />
Zmaja od Bosne 4<br />
71000 Sarajevo<br />
Bosnia<br />
Phone: +387-66 90 60 86<br />
Fax: +387-33 26 27 26<br />
Email: a.hodzic@bmgrp.ba<br />
Internet: www.biomedica.co.at<br />
Bulgaria<br />
Sofbiolife-Biomedica Ltd.<br />
52, Gen. Gurko Str. Floor 5<br />
1000 Sofia<br />
Bulgaria<br />
Phone: +359-2-98 89 151<br />
Fax: +359-2-98 11 883<br />
Email: office@biomedica-bg.eu<br />
Internet: www.biomedica-bg.eu<br />
Canada<br />
CEDARLANE<br />
4410 Paletta Court<br />
Burlington, Ontario, Canada<br />
L7L 5R2<br />
Canada<br />
Phone: 1-800-268-5058 (toll-free)<br />
Fax: +1 289-288-0020<br />
Email: general@cedarlanelabs.com<br />
Internet: www.cedarlanelabs.com<br />
MJS BioLynx Inc.<br />
300 Laurier Blvd.<br />
Brockville, Ontario, Canada<br />
K6V 5W1<br />
Canada<br />
Phone: 1-888-593-5969 (toll-free)<br />
Fax: +1 613 342-1341<br />
Email: sales@biolynx.ca<br />
Internet: www.biolynx.ca<br />
China<br />
Changzhou Hongyu Biomed Ltd.<br />
Tian Yuan Massion, Chang Wu zhong Road,<br />
Changzhou High Tech and Education Park<br />
Changzhou, Jiangsu<br />
China<br />
Phone: +86 519 89 85 93 22<br />
Fax: +86 519 89 85 93 23<br />
Email: info@promocell-china.com<br />
Internet: www.promocell-china.com<br />
Croatia<br />
Biomedica Dijagnostika d.o.o.<br />
V Ravnice 10<br />
10000 Zagreb<br />
Croatia<br />
Phone: +385 1 888 5727<br />
Fax: +385 1 888 5728<br />
Email: biomedica-dijagnostika@bmgrp.hr<br />
Internet: www.biomedica.co.at<br />
Czech Republic<br />
BIOMEDICA CS, s.r.o.<br />
Meteor Centre Office Park “B”<br />
Sokolovská 100/94<br />
186 00 Praha 8<br />
Czech Republic<br />
Phone: +420 2 8393 14 85<br />
Phone: +420 2 8393 36 05<br />
Fax: +420 2 8393 25 07<br />
Email: info@biomedica.cz<br />
Internet: www.biomedica.cz<br />
Denmark<br />
Promo<strong>Cell</strong> GmbH<br />
Sickingenstr. 63/65<br />
69126 Heidelberg<br />
Germany<br />
Phone: +49 6221 - 649 34 0<br />
Fax: +49 6221 - 649 34 40<br />
Email: diana.hops@promocell.com<br />
Internet: www.promocell.com<br />
Finland<br />
Biotop Oy<br />
Tykistökatu 6B 3<br />
20520 Turku<br />
Finland<br />
Phone: +358-2-241 00 99<br />
Fax: +358-2-241 01 11<br />
Email: myynti@biotop.fi<br />
Internet: www.biotop.fi
132<br />
International Distributors<br />
Hungary<br />
Biomedica Hungária Kft.<br />
Ganz u. 16<br />
1027 Budapest<br />
Hungary<br />
Phone: +361-22 53 85 0<br />
Fax: +361-20 12 68 4<br />
Email: biomed@biomedica.hu<br />
Internet: www.biomedica.hu<br />
India<br />
KRISHGEN BioSystems<br />
Unit Nos#318/319<br />
3rd Floor<br />
Shah & Nahar Ind. Premises<br />
Dr E Moses Road<br />
Worli<br />
Mumbai 400018<br />
India<br />
Phone: +91-22-66 37 29 90<br />
Fax: +91-22-66 37 29 91<br />
Email: sales@krishgen.com<br />
Internet: www.krishgen.com<br />
Indonesia<br />
KRISHGEN BioSystems<br />
Unit Nos#318/319<br />
3rd Floor<br />
Shah & Nahar Ind. Premises<br />
Dr E Moses Road<br />
Worli<br />
Mumbai 400018<br />
India<br />
Phone: +91-22-66 37 29 90<br />
Fax: +91-22-66 37 29 91<br />
Email: sales@krishgen.com<br />
Internet: www.krishgen.com<br />
Israel<br />
Biological Industries Ltd.<br />
Kibbutz Beit Haemek, 25115<br />
Israel<br />
Phone: +972-4-996-0595<br />
Fax: +972-4-996-8896<br />
Email: info@bioind.com<br />
Internet: www.bioind.com<br />
Italy<br />
VWR International PBI S.r.l.<br />
Via San Giusto, 85<br />
20153 Milano<br />
Italy<br />
Phone: +39-02-48 77 91<br />
Fax: +39-02-40 09 00 10<br />
Email: info@it.vwr.com<br />
Internet: it.vwr.com<br />
Japan<br />
Funakoshi Co. Ltd.<br />
9-7 Hongo 2-chome<br />
Bunkyo-ku<br />
Tokyo<br />
Japan 113-0033<br />
Phone: +81-3-56 84 16 20<br />
Fax: +81-3-56 84 17 75<br />
Email: reagent@funakoshi.co.jp<br />
Internet: www.funakoshi.co.jp<br />
TaKaRa Bio Inc.<br />
Seta 3-4-1<br />
Otsu, Shiga 520-2193<br />
Japan<br />
Phone: +81-77-543 7247<br />
Fax: +81-77-543 9254<br />
Internet: www.takara-bio.co.jp<br />
Korea<br />
Sungwoo Life Science Co., Ltd.<br />
Samsung BLDG 4th Floor 560-5<br />
Uijeongbu 2Dong Uijeongbu-City<br />
Kyeonggi-Do<br />
South Korea<br />
Phone: +82-2-985-7471<br />
Fax: +82-31-876-9149<br />
Email: sungwoo@sungwools.com<br />
Internet: www.sungwools.com<br />
Macedonia<br />
Biomedika Dijagnostika dooel<br />
Ul. Dame Gruev bb, Zgrada Pelagonka, Kat 2 - Lokal 9<br />
1000 Skopje<br />
Macedonia<br />
Phone: +389 703 62 676<br />
Fax: +389 232 22 404<br />
Email: v.peeva@bmgrp.mk<br />
Internet: www.biomedica.co.at<br />
Malaysia<br />
Biomarketing Services SDN BHD<br />
21, Jalan 4/62A<br />
Bandar Menjalara, Kepong<br />
52200 Kuala Lumpur<br />
Malaysia<br />
Phone: +603 6273 3068<br />
Fax: +603 6272 0093<br />
Email: info@biomarketing.com.my<br />
Internet: www.biomarketing.com.my<br />
Nepal<br />
KRISHGEN BioSystems<br />
Unit Nos#318/319<br />
3rd Floor<br />
Shah & Nahar Ind. Premises<br />
Dr E Moses Road<br />
Worli<br />
Mumbai 400018<br />
India<br />
Phone: +91-22-66 37 29 90<br />
Fax: +91-22-66 37 29 91<br />
Email: sales@krishgen.com<br />
Internet: www.krishgen.com<br />
New Zealand<br />
Banksia Scientific Company<br />
12 Brigade Street<br />
Airport Oaks<br />
Auckland<br />
New Zealand<br />
Phone: +64-9-921 26 26<br />
Fax: +64-9-921 26 27<br />
Email: sales@banksiascientific.co.nz<br />
Internet: www.banksiascientific.co.nz<br />
Life Research Pty Ltd.<br />
Level 1, 5 Lakeside Drive<br />
Burwood East Victoria 3151<br />
Australia<br />
Phone: +61-3-98 03 00 45<br />
Fax: +61-3-86 48 58 41<br />
Email: info@liferesearch.com<br />
Internet: www.liferesearch.com<br />
Norway<br />
Promo<strong>Cell</strong> GmbH<br />
Sickingenstr. 63/65<br />
69126 Heidelberg<br />
Germany<br />
Phone: +49 6221 - 649 34 0<br />
Fax: +49 6221 - 649 34 40<br />
Email: diana.hops@promocell.com<br />
Internet: www.promocell.com
International Distributors 133<br />
Poland<br />
Biomedica Poland sp. z.o.o.<br />
Ul. Raszyńska 13<br />
05-500 Piaseczno<br />
Poland<br />
Phone: +48-22 73 75 996<br />
Fax: +48-22 73 75 994<br />
Email: biomedica@bmgrp.pl<br />
Internet: www.biomedica.pl<br />
Portugal<br />
Labclinics, S.A.<br />
c/Industria 54<br />
08025 Barcelona<br />
Spain<br />
Phone: +34-93-446 47 00<br />
Fax: +34-93-348 10 39<br />
Email: info@labclinics.com<br />
Internet: www.labclinics.com<br />
Romania<br />
CYBER SRL Romania<br />
Str. Smaranda Braescu, nr. 20D<br />
Sector 1, Cod 014203<br />
70941 Bucharest<br />
Romania<br />
Phone: +40-37 27 66 844<br />
Fax: +40-21 23 22 204<br />
Email: dan.pavel@cybermed.ro<br />
Internet: www.cyber-srl.ro<br />
Serbia<br />
BIOMEDICA MP d.o.o.<br />
Cara Dusana 214<br />
11080 Zemun<br />
Serbia<br />
Phone: +381 11 6301 882<br />
Fax: +381 11 6301 883<br />
Email: office@biomedicamp.com<br />
Internet: www.biomedica.co.at<br />
Singapore<br />
Biomed Diagnostics Pte. Ltd.<br />
18 Boon Lay Way<br />
#05-105-108 TradeHub 21<br />
Singapore 609966<br />
Phone: +65-6-298 43 47<br />
Fax: +65-6-298 47 23<br />
Email: sales@biomed.com.sg<br />
Internet: www.biomed.com.sg<br />
Singlab Technologies Pte. Ltd.<br />
1003 Bukit Merah Central #07-50<br />
INNO ° CENTRE<br />
Singapore 159836<br />
Phone: +65-6-272 07 88<br />
Fax: +65-6-270 71 28<br />
Email: Promo<strong>Cell</strong>@singlab.com.sg<br />
Internet: www.singlab.com.sg<br />
Slovakia<br />
Biomedica Slovakia s.r.o.<br />
Drobného 27<br />
84102 Bratislava<br />
Slovakia<br />
Phone: +421-2-69 30 99 01<br />
Fax: +421-2-69 30 99 08<br />
Email: biomedica@biomedica.sk<br />
Internet: www.biomedica.sk<br />
Slovenia<br />
Biomedis M.B.<br />
Slokanova ulica 12<br />
2000 Maribor<br />
Slovenia<br />
Phone: +386-2-47 16 300<br />
Fax: +386-2-47 16 304<br />
Email: info@biomedis-mb.si<br />
Internet: www.biomedis-mb.si<br />
South Korea<br />
Sungwoo Life Science Co., Ltd.<br />
Samsung BLDG 4th Floor 560-5<br />
Uijeongbu 2Dong Uijeongbu-City<br />
Kyeonggi-Do<br />
South Korea<br />
Phone: +82-2-985-7471<br />
Fax: +82-31-876-9149<br />
Email: sungwoo@sungwools.com<br />
Internet: www.sungwools.com<br />
Spain<br />
Labclinics, S.A.<br />
c/Industria 54<br />
08025 Barcelona<br />
Spain<br />
Phone: +34-93-446 47 00<br />
Fax: +34-93-348 10 39<br />
Email: info@labclinics.com<br />
Internet: www.labclinics.com<br />
Sri Lanka<br />
KRISHGEN BioSystems<br />
Unit Nos#318/319<br />
3rd Floor<br />
Shah & Nahar Ind. Premises<br />
Dr E Moses Road<br />
Worli<br />
Mumbai 400018<br />
India<br />
Phone: +91-22-66 37 29 90<br />
Fax: +91-22-66 37 29 91<br />
Email: sales@krishgen.com<br />
Internet: www.krishgen.com<br />
Sweden<br />
Mediqip AB<br />
Förrådsvägen 10<br />
141 46 Huddinge<br />
Sweden<br />
Phone: +46-8-448 10 10<br />
Fax: +46-8-448 10 75<br />
Email: info@mediqip.se<br />
Internet: www.mediqip.se<br />
Taiwan<br />
Biochiefdom International Co. Ltd.<br />
1F, No 50, Yishou St.<br />
Wenshan District<br />
Taipei 11659<br />
Taiwan<br />
Phone: +886-2-29 37 00 88<br />
Fax: +886-2-29 37 97 17<br />
Email: info@bio-chief.com.tw<br />
Internet: www.bio-chief.com.tw<br />
Thailand<br />
Biomed Diagnostics (Thailand) Co. Ltd.<br />
96/38 Moo 6 Bangkruai-Sainoi Rd<br />
Bangkruai<br />
Nontaburi, 11130<br />
Thailand<br />
Phone: +66-2-879 60 26<br />
Fax: +66-2-879 60 65<br />
Email: nan@biomedthai.com<br />
Internet: www.biomedthai.com
134<br />
Terms and Conditions<br />
Terms and Conditions<br />
General Terms<br />
1. These conditions of sale and delivery<br />
apply to all contracts of supply between<br />
us and our customers, even when in individual<br />
cases they are not referred to.<br />
2. A customer’s inconsistent conditions<br />
of purchase shall have no validity, even<br />
when we do not expressly reject such<br />
conditions.<br />
3. The customer is deemed to agree to<br />
these conditions of sale and delivery<br />
upon unconditional acceptance of the<br />
goods supplied.<br />
4. Rights of the customer arising during<br />
the course of business with us are not<br />
transferable.<br />
5. Any agreement that differs from these<br />
conditions of sale and delivery, or any<br />
recognition of the customer’s contradictory<br />
purchase conditions, requires our<br />
express confirmation in writing.<br />
Offers<br />
1. In the absence of a declaration to the<br />
contrary, we are not bound by any offers<br />
we make.<br />
2. Supply contracts only take effect when<br />
confirmed by us in writing.<br />
3. Any inquiries or complaints concerning<br />
confirmation are to be made promptly in<br />
writing.<br />
4. Assurances and assured properties<br />
only exist when they are expressly described<br />
as such. The descriptions and<br />
other statements in our catalogues and<br />
price lists are accurate to the best of our<br />
knowledge and belief. Under no circumstances<br />
may any assurances as to properties<br />
be derived from such descriptions<br />
and statements.<br />
Prices<br />
1. Our prices quoted in the current price<br />
list are net prices, VAT (value added tax)<br />
not included.<br />
2. VAT is charged according to the applicable<br />
laws in effect.<br />
3. Charges for delivery, cooling and insurance<br />
are indicated in the valid price<br />
list.<br />
4. Promo<strong>Cell</strong> prices are subject to change<br />
without prior notice.<br />
Delivery<br />
1. Delivery times will be specified after<br />
careful co-ordination. They are however<br />
not legally binding. Claims for compensation<br />
due to delayed or non-delivery are<br />
excluded.<br />
2. If a delivery date specified by us is<br />
exceeded by more than two weeks, the<br />
customer is entitled to give us a further<br />
two week time limit for delivery. Should<br />
the delivery not be carried out by the<br />
end of the additional two week delivery<br />
period, the customer is entitled to rescind<br />
the contract. The rescission must<br />
be made promptly and in writing, at the<br />
latest within a week of the expiry of the<br />
additional two week delivery period.<br />
3. All deliveries take place at the customer’s<br />
risk. The passing of risk takes place<br />
on the handing over of the goods to the<br />
carrier or haulier. The careful choice of<br />
dispatch method and means of transport<br />
is at our discretion with exclusion of any<br />
and all liability.<br />
4. Deliveries in part are permitted. They<br />
are to be regarded as separate deliveries.<br />
Payment<br />
1. Payment is to be made without any<br />
deductions within 14 days of delivery<br />
and remittance of the bill.<br />
2. Default of payment will result in legal<br />
proceedings upon the expiry of the payment<br />
period without further warning. In<br />
this case, or in the case of a warning, we<br />
are entitled to interest for late payment<br />
at the current normal bank borrowing<br />
rate, or alternatively at a rate of 5%<br />
above the current discount rate of the<br />
Deutsche Bundesbank.<br />
3. A cash discount is only permitted<br />
when expressly stated on the bill and<br />
when payment is made within the stated<br />
period.<br />
4. An offset against or demand for<br />
payment is only permitted when the<br />
customer has an undisputed or legally<br />
established counterclaim.<br />
5. If our conditions of payment are not<br />
complied with or if justifiable doubts<br />
exist regarding the customer’s ability or<br />
willingness to pay, we are entitled to<br />
rescind the contract and to demand the<br />
return of the delivered goods, the costs<br />
of delivery to be borne by the customer.<br />
In addition, all time outstanding claims<br />
will become due.<br />
Justifiable doubts referred to above<br />
include, but are not restricted to: unfavourable<br />
information, deterioration of<br />
financial circumstances, initiation of insolvency<br />
or bankruptcy proceedings, execution<br />
measures against the customer,<br />
protest of bills, and default of payment<br />
for any other reason.<br />
6. If the customer does not take delivery<br />
of properly delivered goods, we are<br />
entitled, after setting an additional time<br />
limit, to rescind the contract, or to damages<br />
for non-compliance with the contract.<br />
In the latter case we are entitled to<br />
demand either damages of 25% of the<br />
sales price without proof, or the actual<br />
amount of damages.<br />
Complaint<br />
1. Any complaint regarding the goods,<br />
such as defects, lack of assured properties,<br />
wrong delivery and over- or underdelivery<br />
are, after immediate discontinuation<br />
of use, to be reported in writing.<br />
2. In the case of wrong delivery, the<br />
goods will be taken back at our expense<br />
and replaced with the goods ordered.<br />
Excess deliveries may similarly be sent<br />
back to us at our expense. In the case of<br />
under delivery, the customer may choose<br />
between a reduction in payment or complete<br />
fulfilment of the order.<br />
3. In the case of delivery of faulty goods<br />
we are entitled to replace the faulty<br />
goods with goods free of faults. Should<br />
the replacement delivery also be faulty,<br />
the customer may choose between a<br />
reduction in payment or cancellation of<br />
the contract. Claims for compensation<br />
are excluded except in the case of lack of<br />
assured properties.<br />
4. The right to complain expires in the<br />
case of obvious or apparent defects two<br />
weeks after receipt of the goods, and<br />
in the case of non-apparent defects six<br />
months after receipt of the goods.<br />
5. Goods may only be returned by arrangement<br />
with us and with the issue<br />
of a return number. The goods must be
Terms and Conditions 135<br />
properly packed. If returned goods are<br />
damaged due to the fault of the customer,<br />
the customer is obliged to pay<br />
compensation for such damage.<br />
6. In the case of delivery to traders the<br />
customer is to inspect the goods without<br />
delay upon receipt and to report<br />
any complaints to us immediately. If the<br />
customer fails to make such a report<br />
the goods will be deemed approved. In<br />
the case of a fault that is not apparent<br />
upon inspection, the complaint must be<br />
reported immediately upon discovery of<br />
the fault, otherwise the goods will be<br />
deemed approved as regards such fault.<br />
Use of Goods<br />
1. Goods delivered by us are intended<br />
exclusively for the purposes of research<br />
and may not be used on human beings,<br />
animals, in the household, or for any<br />
private use. We emphasise that the use<br />
of our products for diagnostic or therapeutic<br />
purposes is subject to current legal<br />
regulations.<br />
2. We do not accept any liability for the<br />
improper use of our products. We deliver<br />
only to commercial enterprises, resalers<br />
and public research, development and<br />
teaching establishments. Legal regulations<br />
require us to carefully check orders<br />
and to refuse delivery when there are<br />
signs of improper use of our products.<br />
Orders from students or other private<br />
individuals must be given from an official<br />
office of their institute or firm.<br />
3. We expressly oppose research or any<br />
uses which run counter to ethical, legal<br />
or political standards or principles within<br />
Germany and/or the import country, or<br />
whose ethical justification is the subject<br />
of public debate and dispute in the respective<br />
country (e.g. certain cloning<br />
experiments).<br />
We do not accept any liability for such<br />
research or use by our customers, or<br />
by third parties who have received our<br />
products from our customers.<br />
Liability<br />
1. All products delivered by us are only<br />
to be handled by trained personnel who<br />
are well aware of any potential dangers.<br />
Should any liability for compensation<br />
arise as a result of damage caused by<br />
breach of this condition, the customer<br />
shall be liable to pay any such compensation.<br />
In all cases, current safety regulations<br />
are to be observed, especially in the<br />
case of cells of human or animal origin,<br />
which always represent a potential risk<br />
of infection. The lack of a danger symbol<br />
does not mean that the substances concerned<br />
are harmless.<br />
2. We accept no responsibility for damage,<br />
loss, or injury caused by incorrect<br />
handling or use in the household, on<br />
humans, or on animals. In as far as we<br />
have made confirmation of delivery dependent<br />
upon a specified use of certain<br />
products, the customer accepts full liability<br />
for any damage suffered by us as<br />
a result of the customer’s incorrect use<br />
of the product. In the case of products<br />
which may only be used in accordance<br />
with legal or official regulations, the customer’s<br />
order simultaneously counts as<br />
a declaration that the products will be<br />
used for a specific purpose in the above<br />
sense.<br />
3. Our customers are required without<br />
exception to apply the laboratory guidelines<br />
of the professional association of<br />
the chemical industry when dealing with<br />
our products.<br />
4. Passing on of our products to private<br />
individuals is not permitted. If as a result<br />
of a breach of this condition for whatever<br />
reason we should receive a claim for<br />
compensation from a private individual,<br />
we shall have a right of redress against<br />
the customer.<br />
Indemnification<br />
1. In addition to section “complaint”,<br />
claims by the customer are excluded, in<br />
so far as they are not based upon intention<br />
or gross negligence.<br />
2. In the case of delivery to a trader this<br />
exclusion also applies to any damage<br />
caused by the intention or gross negligence<br />
of our business associates, carriers<br />
or employees. We accept no liability<br />
for damage caused by the behaviour of<br />
companies or individuals which sell our<br />
products.<br />
3. The sale of our products under their<br />
trade names for further commercial use<br />
does not mean that these products and<br />
their trade names may be freely used<br />
in general circulation. Some products,<br />
their production and use or their trade<br />
names are patented or protected trade<br />
names. The further commercial use of<br />
such products and their trade names occurs<br />
as regards a third party under the<br />
exclusive responsibility and liability of<br />
the customer.<br />
Miscellaneous<br />
1. German law shall apply to all contracts<br />
entered into by us, including those for<br />
deliveries abroad. We expressly reject the<br />
application of the Vienna UN-agreement<br />
relating to international sales contracts<br />
of 11. 04. 80.<br />
2. In the case of contracts with traders<br />
who do not belong to the traders specified<br />
in §4 of the code of commercial law,<br />
the place for fulfilment of contracts and<br />
the court of jurisdiction shall be Heidelberg.<br />
3. If any of the above conditions is held<br />
to be invalid, the validity of any of the<br />
other conditions shall not be affected.<br />
4. Only the German version of this Terms<br />
of Delivery and Payment is valid. This<br />
translation shall only serve as assistance<br />
for our customers. You can get a German<br />
version on request.<br />
Promo<strong>Cell</strong> GmbH<br />
Sickingenstr. 63/65<br />
69126 Heidelberg<br />
Germany<br />
Managing Director: Dirk Hüttner<br />
AG Mannheim HRB 335480
136<br />
Ordering and Technical Support<br />
Ordering and Technical Support<br />
Ordering Information<br />
To place an order please use any of the<br />
following convenient options:<br />
Phone Fax Online<br />
Our customer service representatives are<br />
ready to assist you with ordering Monday<br />
through Friday<br />
USA/Canada<br />
1 – 866 – 251 – 2860 (toll free)<br />
Germany<br />
0800 – 776 66 23 (toll free)<br />
France<br />
0800 90 93 32 (toll free)<br />
United Kingdom<br />
0800 – 96 03 33 (toll free)<br />
Switzerland<br />
+49 6221 – 649 34 0<br />
Netherlands<br />
+49 6221 – 649 34 0<br />
Other Countries<br />
+49 6221 – 649 34 0<br />
Please copy the fax order form on the next<br />
page and send it to the following fax numbers:<br />
USA/Canada<br />
1 – 866 – 827 – 9219 (toll free)<br />
Germany<br />
0800 – 100 83 06 (toll free)<br />
France<br />
0800 90 27 36 (toll free)<br />
United Kingdom<br />
0800 – 169 85 54 (toll free)<br />
Switzerland<br />
+49 6221 – 649 34 40<br />
Netherlands<br />
+49 6221 – 649 34 40<br />
Other Countries<br />
+49 6221 – 649 34 40<br />
Online ordering is available 24 hours/<br />
day, 7 days per week. Our online ordering<br />
system is secure, fast, and convenient.<br />
Check out www.promocell.com for more<br />
information.<br />
Email<br />
Please send an e-mail to<br />
order@promocell.com and include the<br />
following information:<br />
Catalog number, name, size and quantity<br />
Shipping and billing address<br />
Contact person`s name and telephone<br />
number<br />
Promo<strong>Cell</strong> directly supplies to Belgium,<br />
Canada, France, Germany, Ireland,<br />
Liechtenstein, Luxembourg, Monaco,<br />
Netherlands, Switzerland, United Kingdom,<br />
and the United States.<br />
All deliveries are carried out using our<br />
recommended carrier, unless an alternative<br />
agreement has been arranged<br />
with the customer. Delivery time and<br />
shipment costs are extremely favorable.<br />
Our local sales representatives<br />
and our multilingual customer service<br />
are looking forward to serving you!<br />
For current information about Promo<strong>Cell</strong><br />
distributors see www.promocell.com or<br />
contact us via e-mail or phone.<br />
Technical Support<br />
Providing excellent technical support<br />
for our products is our top priority. Our<br />
technical service team relies on years of<br />
laboratory experience to assist you with<br />
product selection and advice, to maximize<br />
product performance.<br />
You may reach our technical support via<br />
telephone/fax (see numbers on the reverse<br />
side of the catalog) or by sending<br />
an e-mail to: tech.support@promocell.com<br />
Technical support is completed by our<br />
online Knowledge Base www.promocell.<br />
com/knowledge-base which provides<br />
reference literature, application notes,<br />
and a library of technical questions and<br />
answers related to our primary cells and<br />
media.<br />
Product Use<br />
Products are sold for research use only<br />
and are not intended for use in diagnostic<br />
procedures or for therapeutic and<br />
medical purposes.<br />
Trademarks Used in this Catalog<br />
The trademarks, logos, and service<br />
marks (collectively the “Trademarks”)<br />
displayed in this catalog, including but<br />
not limited to those contained in each<br />
of the list below are trademarks or registered<br />
trademarks of Promo<strong>Cell</strong> GmbH<br />
in Europe, the U.S. and other countries.<br />
All other brands and product names are<br />
trademarks or registered trademarks<br />
of their respective companies. Nothing<br />
contained in this catalog should be<br />
construed as granting, by implication,<br />
estoppel, or otherwise, any license or<br />
right to use any trademarks displayed<br />
in the catalog without the permission<br />
of Promo<strong>Cell</strong> GmbH or such third<br />
party that may own the trademarks<br />
displayed in the catalog. The misuse of<br />
the trademarks displayed in the catalog,<br />
or any other content in the catalog, is<br />
strictly prohibited. Please be advised that<br />
Promo<strong>Cell</strong> GmbH will aggressively enforce<br />
its intellectual property rights to<br />
the fullest extent of the law, including<br />
the seeking of criminal prosecution.<br />
Promo<strong>Cell</strong> (Promo<strong>Cell</strong> GmbH)<br />
PromoFectin (Promo<strong>Cell</strong> GmbH)<br />
PromoFluor (Promo<strong>Cell</strong> GmbH)<br />
PromoKine (Promo<strong>Cell</strong> GmbH)<br />
DetachKit (Promo<strong>Cell</strong> GmbH)<br />
SupplementPack (Promo<strong>Cell</strong> GmbH)<br />
SupplementMix (Promo<strong>Cell</strong> GmbH)<br />
<strong>Cell</strong> <strong>Culture</strong> Assistant (Promo<strong>Cell</strong> GmbH)<br />
ABI Prism ®<br />
<strong>Cell</strong> Lab Quanta SC ®<br />
Cepheid Smart Cycler ®<br />
LightCycler ®<br />
Mastercycler ®<br />
RNAlater ®
Fax Order Form<br />
Shipping Address<br />
Invoice Address (if different from shipping address)<br />
Mr. Ms. Dr. Prof.<br />
Mr.<br />
Ms. Dr. Prof.<br />
First Name<br />
First Name<br />
Last Name<br />
Last Name<br />
Company / University<br />
Company / University<br />
Department / Institute<br />
Department / Institute<br />
Street, No<br />
Street, No<br />
ZIP, City<br />
ZIP, City<br />
Country<br />
Country<br />
Phone<br />
E-mail<br />
Order Details<br />
Catalog Number Product Name Quantity<br />
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Promo<strong>Cell</strong> GmbH<br />
Sickingenstr. 63/65<br />
69126 Heidelberg<br />
Germany<br />
USA/Canada<br />
Phone: 1 – 866 – 251 – 2860 (toll free)<br />
Fax: 1 – 866 – 827 – 9219 (toll free)<br />
Deutschland<br />
Telefon: 0800 – 776 66 23 (gebührenfrei)<br />
Fax: 0800 – 100 83 06 (gebührenfrei)<br />
France<br />
Téléphone: 0800 90 93 32 (ligne verte)<br />
Téléfax: 0800 90 27 36 (ligne verte)<br />
United Kingdom<br />
Phone: 0800 – 96 03 33 (toll free)<br />
Fax: 0800 – 169 85 54 (toll free)<br />
Switzerland<br />
Phone: +49 6221 – 649 34 0<br />
Fax: +49 6221 – 649 34 40<br />
Netherlands<br />
Phone: +49 6221 – 649 34 0<br />
Fax: +49 6221 – 649 34 40<br />
Other Countries<br />
Phone: +49 6221 – 649 34 0<br />
Fax: +49 6221 – 649 34 40<br />
Email:<br />
info@promocell.com<br />
Promoting sustainable<br />
forest management<br />
www.pefc.org<br />
www.promocell.com