Contingency Plan for Hawaiian Monk Seal Unusual Mortality Events
Contingency Plan for Hawaiian Monk Seal Unusual Mortality Events
Contingency Plan for Hawaiian Monk Seal Unusual Mortality Events
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A-1<br />
Introduction<br />
HAWAIIAN MONK SEAL SPECIMEN COLLECTION PROTOCOL<br />
Jan 29 2003/RCB<br />
The following protocol describes the collection, processing, preservation, packaging, and shipment<br />
techniques <strong>for</strong> specimens collected from <strong>Hawaiian</strong> monk seals. Strict adherence to this protocol is<br />
necessary to ensure that each specimen is handled and preserved in an appropriate and standardized<br />
manner. There is an optimal chance to determine the cause and nature of disease and to assess its<br />
significance, and the resulting analyses are valid and useful to the management of this endangered species.<br />
Additional references are provided in Section IV.<br />
SPECIMEN COLLECTION: POPULATION ASSESSMENT FIELD CAMPS<br />
Specimens to be collected during population assessment work include:<br />
Scats<br />
Spews<br />
Endoparasites<br />
Skin tissue plugs<br />
Molt samples<br />
Placentas<br />
Ticks<br />
Skulls<br />
Additional specimens to be collected during assessment work include specimens collected from necropsies<br />
(see <strong>Hawaiian</strong> <strong>Monk</strong> <strong>Seal</strong> Necropsy Protocol). Strict adherence to the necropsy protocol is necessary in<br />
order to obtain the appropriate specimens from dead monk seals.<br />
I. Specimen Labeling<br />
All specimen labels must include the following in<strong>for</strong>mation:<br />
<strong>Seal</strong> ID<br />
Date collected (YYMMDD <strong>for</strong>mat)<br />
Specimen Type/ Sub-type<br />
Specimen #/sub #, Island/Atoll<br />
Include the following if there is room (container larger than cryovial):<br />
<strong>Seal</strong> size and sex (if known)<br />
Preservation method<br />
Collector initials<br />
When filling out labels, please write legibly and distinguish between "zero" and the letter "0" by drawing a<br />
line through the zero. Unless noted otherwise, all specimens should have both an inner and outer label.<br />
Inner labels<br />
1. Specimens in jars, glass vials, whirlpaks, and ziplocs should have preprinted waterproof labels<br />
inside containers in case the outer labels fall off or become illegible. Fill out the labels with pencil<br />
and be sure to position them inside containers so that labels can be read from the outside<br />
without having to remove the labels to read them.<br />
2. Specimens in cryovials (blood, serum, plasma, certain blubber samples, etc) and teflon vials<br />
should not have inner labels.
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